Rw4G022470

Serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0000660
Physical Location & Seq
Forward (+)
47860217 .. 47865730
5514 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G022470.1

Sequence Viewer

Length: 2304 bp
ATGGACACTCCGCCGCCGCCGGAGGAGCTGCTGAAGAAGATCCAGGTGCTGGAGGAGGGTCAGGAGCACCTGAAGCAGGAGATGTCCAAGCTCCGGAACAACTCCGATGCTAAGTCCGAGCACCCGAGGGCCCACTCGGTCTCGCCGCAGCGGTCCAGATTCTCGTCTTTGCCCAAGAGGAGAGTCGCCGGAGCCGGAGCCGGAGCCGACGCGGTGGGTTGGAAGAGAGGGTCTACTTCGTTTCGGCATTTTTCGCCGCTGCAGAGGGAGAGCCGCAGCCATGACCCTCCGACTGGCGGCAGCGGCCGCGCCGTCGATGATTTTAGAGGTGGAGGTGGTAGCGGGCCGTCGGCGGTGAACTTTACTGATAGGCAGTATCTGAATATATTGCAGTCCATTGGGCAGTCTGTGCATATTTTTGATCCCCATGGTCGTATAATTTACTGGAACAAAACAGCTGAAGCTATGTATGGTTATTCAGCGGCAGAGGCCCTGGGACGGGATCCCATTGAGTTCTTAACAGATCCCCAGGACCATGTTGTAGGACATAATATAATCCAAAGAGTCACCAAGGGGGAGAGCTGGACAGGGCACTTCCCTGTCAAGAACAAAAAGGGGGAGAGATTTACAGCTACTATAACGAATACTCCATTCTATGATGATGATGGCACTTTTATTGGGGTTATTTGTGTGTCAAGTGATACGAGGCCATTTCAAGAAATGAGGACGCACCCTGAACCAGATTCAAGCTTCAGTCGTACTAAAGCTACTGTTACAACTAAACTTGGTCTTGATCCTCAGCAGCCTCTGCAAACTGCAATTGCATCAAAAATTACAAATTTGGCATCCAAGATGAGCAACAAAGTCAAGTCGAAAATTCGGGCAGGAGAAAACTATATGGATCGGGAAGGTGGGAGTGTAGATAGTCATCATTCTGATCATGGTTCGGACTATGACCATCGGGAGGATGCAAATTCAAGTGGGGCTAGCACACCTAGAGGAGATATAGCCCCATCTCCTTTTGGTGTGTTTTCCCAGATGGATGAAAAGTCTCCAGGAAAACCTTCCAGAGATTCTGGCGATGAGAGTGAAGGAAAACCACCAATGCACAAGGTAATATCCTCAAAGGCAGAAGCATGGATTGGTAAGAAGGGGATAACTTGGCCATGGAAAGGAAATGAGCGAGAAGGGTCAGAGGTTAAGACCCCTCGTTTTCTTTGGCCTTGGTTGCAAAATGATCAAGAGAATGATCCAGTTCCTCAGAAGAATAATTGTGGTTTAAAACCTGACAATTACGCGACTGAAAGTAATCGACATGCAACTAATGAGGCTTCAGGATCCTGGACCTCCTCATTTAATGTTAACAGCACAAGCAGTGCCAGCAGCTGTGGAAGCACCAGCAGTACTGTGAATAAGGTAGACGTGGACACTGATTGCTTGGATTACGAAATCTTGTGGGAAGACTTGATGATTGGAGAACAGATTGGGCAAGGTTCATGTGGAACTGTATACCATGGTCTGTGGTACGGATCAGATGTTGCTGTCAAGGTATTCTCCAGGCAAGAATATTCAGAAGATGTGATACTTTCCTTCAGACAAGAGGTATCTCTTATGAAAAGGCTTCGACATCCAAATGTTCTGCTTTTCATGGGAGCTGTGACTTCTCCCGAGCGTCTTTGCATCGTAACAGAGTTCCTCCCACGTGGAAGTTTGTTTCGGTTACTACAAAGAAGCACAGCAAAACTAGACTGGAGACGACGTGTTCTTATGGCTCTGGATATAGCACGAGGCATGAATTATCTTCATCATTTCAACCCTCCAATCATCCATCGAGATCTGAAGTCTTCAAATCTCCTAGTTGATAAGAACTGGACTGTCAAGGTTGGTGATTTTGGTCTTTCACGTCTAAAGCATGAAACATTTCTTACAACCAAGACTGGGAGGGGAACGCCCCAATGGATGGCACCAGAAGTTCTACGCAATGAACCCTCAGATGAGAAGTCGGATGTATATAGCTACGGAGTGATATTGTGGGAGCTTGTTACTGAAAAGATCCCTTGGGATAATCTAAACTCAATGCAGGTGATTGGAGCTGTAGGGTTCATGAACCAAAGACTAGAGATCCCAAAAGATATTGACCCGCAATGGATTTCTTTAATTGAGAGCTGCTGGCAAAGTGATCCTGCTAGCCGGCCAACATTTCAAGAGCTGCTGGAAAAGCTTCGAGATCTGCAGAGACAATATGCTCTTCAATTCCAAGCAGCGCGGTCCGCCGCAAGTGATAGCACCCAAAAAGAACTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001666 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004712 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005789 GO:0006082 GO:0006464 GO:0006468 GO:0006629 GO:0006720 GO:0006721 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0008299 GO:0008610 GO:0009058 GO:0009628 GO:0009685 GO:0009686 GO:0009719 GO:0009723 GO:0009725 GO:0009743 GO:0009744 GO:0009746 GO:0009750 GO:0009756 GO:0009966 GO:0009968 GO:0009987 GO:0010033 GO:0010035 GO:0010038 GO:0010039 GO:0010104 GO:0010105 GO:0010182 GO:0010646 GO:0010648 GO:0010817 GO:0012505 GO:0016020 GO:0016053 GO:0016101 GO:0016102 GO:0016114 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0019752 GO:0023051 GO:0023052 GO:0023057 GO:0031984 GO:0034284 GO:0034285 GO:0036211 GO:0036293 GO:0040034 GO:0042175 GO:0042221 GO:0042445 GO:0042446 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043436 GO:0044237 GO:0044238 GO:0044249 GO:0044255 GO:0044260 GO:0044267 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044425 GO:0044432 GO:0044444 GO:0044446 GO:0044464 GO:0046394 GO:0046777 GO:0048506 GO:0048509 GO:0048510 GO:0048519 GO:0048523 GO:0048580 GO:0048583 GO:0048585 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051239 GO:0051302 GO:0051716 GO:0065007 GO:0065008 GO:0070297 GO:0070298 GO:0070482 GO:0070887 GO:0071241 GO:0071248 GO:0071281 GO:0071310 GO:0071322 GO:0071704 GO:0098827 GO:0140096 GO:1901564 GO:1901576 GO:1901700 GO:1901701 GO:1902531 GO:1902532 GO:2000026 GO:2000035 GO:2000069 GO:2000280
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PAS PAS_9 Pkinase_Tyr

Protein Analysis

767

Amino Acids

85.78

Weight (kDa)

6.66

Isoelectric Point (pI)

50.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PAS PF00989 125 - 234 1.3e-13 PAS fold
PAS_8 PF13188 125 - 178 5.9e-06 PAS domain
PAS_4 PF08448 131 - 235 7.2e-13 PAS fold
PAS_9 PF13426 139 - 234 1.1e-12 PAS domain
PAS_3 PF08447 146 - 231 9.2e-07 PAS fold
PK_Tyr_Ser-Thr PF07714 490 - 741 8.3e-68 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 492 - 739 1.4e-57 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 2071
Acc36I ACCTGC 1 cut(s) 2071
AccB1I GGYRCC 1 cut(s) 1963
AccB7I CCANNNNNTGG 2 cut(s) 49, 1960
AccI GTMKAC 3 cut(s) 233, 1419, 1509
AccII CGCG 4 cut(s) 212, 309, 1298, 2266
AccIII TCCGGA 1 cut(s) 93
AcoI YGGCCR 3 cut(s) 304, 1163, 2192
AcsI RAATTY 3 cut(s) 838, 876, 973
AcuI CTGAAG 7 cut(s) 53, 92, 480, 736, 1317, 1576, 1859
AcvI CACGTG 1 cut(s) 1703
AfaI GTAC 3 cut(s) 760, 1405, 1526
AflIII ACRYGT 1 cut(s) 1759
AgsI TTSAA 7 cut(s) 716, 747, 978, 1813, 1848, 2204, 2252
AjiI CACGTC 3 cut(s) 1423, 1760, 1904
AjnI CCWGG 6 cut(s) 42, 492, 528, 1054, 1340, 1556
AjuI GAANNNNNNNTTGG 2 cut(s) 1125, 1157
Alw21I GWGCWC 2 cut(s) 69, 123
Alw26I GTCTC 4 cut(s) 145, 1056, 1747, 2230
AlwNI CAGNNNCTG 4 cut(s) 49, 379, 808, 1386
Ama87I CYCGRG 2 cut(s) 124, 1667
Aor13HI TCCGGA 1 cut(s) 93
AoxI GGCC 8 cut(s) 129, 304, 344, 489, 707, 1163, 1220, 2192
ApaI GGGCCC 1 cut(s) 133
ApoI RAATTY 3 cut(s) 838, 876, 973
Asp700I GAANNNNTTC 3 cut(s) 1063, 1920, 2220
AspLEI GCGC 2 cut(s) 311, 2266
AspS9I GGNCC 8 cut(s) 129, 130, 153, 344, 490, 532, 1344, 2268
AsuHPI GGTGA 4 cut(s) 367, 559, 1898, 2095
AsuNHI GCTAGC 2 cut(s) 986, 2186
AvaI CYCGRG 2 cut(s) 124, 1667
AvaII GGWCC 4 cut(s) 153, 532, 1344, 2268
BaeGI GKGCMC 2 cut(s) 133, 594
BalI TGGCCA 1 cut(s) 1165
BamHI GGATCC 2 cut(s) 502, 1337
BanI GGYRCC 1 cut(s) 1963
BanII GRGCYC 1 cut(s) 133
BarI GAAGNNNNNNTAC 2 cut(s) 1566, 1598
BauI CACGAG 1 cut(s) 1785
BbrPI CACGTG 1 cut(s) 1703
BbsI GAAGAC 2 cut(s) 1467, 1836
Bbv12I GWGCWC 2 cut(s) 69, 123
BbvCI CCTCAGC 1 cut(s) 798
BccI CCATC 6 cut(s) 659, 966, 1021, 1033, 1836, 1954
BceAI ACGGC 2 cut(s) 296, 331
BciT130I CCWGG 6 cut(s) 44, 494, 530, 1056, 1342, 1558
BclI TGATCA 2 cut(s) 937, 1237
BcoDI GTCTC 4 cut(s) 145, 1056, 1747, 2230
BfaI CTAG 6 cut(s) 987, 996, 1745, 1856, 2117, 2187
BfmI CTRYAG 4 cut(s) 260, 2094, 2231, 2300
BfuAI ACCTGC 1 cut(s) 2071
BglII AGATCT 2 cut(s) 1834, 2227
BmcAI AGTACT 1 cut(s) 1405
Bme1390I CCNGG 6 cut(s) 44, 494, 530, 1056, 1342, 1558
Bme18I GGWCC 4 cut(s) 153, 532, 1344, 2268
BmeT110I CYCGRG 2 cut(s) 124, 1667
BmgBI CACGTC 3 cut(s) 1423, 1760, 1904
BmgT120I GGNCC 8 cut(s) 129, 130, 153, 344, 490, 532, 1344, 2268
BmiI GGNNCC 7 cut(s) 131, 193, 199, 205, 504, 1339, 1965
BmrFI CCNGG 6 cut(s) 44, 494, 530, 1056, 1342, 1558
BmrI ACTGGG 1 cut(s) 1947
BmsI GCATC 5 cut(s) 97, 833, 854, 958, 1689
BmtI GCTAGC 2 cut(s) 990, 2190
BmuI ACTGGG 1 cut(s) 1947
BpiI GAAGAC 2 cut(s) 1467, 1836
BpmI CTGGAG 4 cut(s) 71, 1038, 1540, 1771
Bpu10I CCTNAGC 1 cut(s) 798
BsaAI YACGTR 1 cut(s) 1703
BsaBI GATNNNNATC 1 cut(s) 927
BsaI GGTCTC 1 cut(s) 145
BsaWI WCCGGW 1 cut(s) 93
BsaXI ACNNNNNCTCC 4 cut(s) 631, 661, 1744, 1774
Bse118I RCCGGY 1 cut(s) 2190
Bse1I ACTGG 6 cut(s) 298, 449, 1253, 1754, 1874, 1942
Bse3DI GCAATG 2 cut(s) 1987, 2150
Bse8I GATNNNNATC 1 cut(s) 927
BseAI TCCGGA 1 cut(s) 93
BseBI CCWGG 6 cut(s) 44, 494, 530, 1056, 1342, 1558
BseGI GGATG 7 cut(s) 845, 973, 1048, 1627, 1824, 1965, 2011
BseJI GATNNNNATC 1 cut(s) 927
BseMI GCAATG 2 cut(s) 1987, 2150
BseMII CTCAG 3 cut(s) 812, 1274, 2004
BseNI ACTGG 6 cut(s) 298, 449, 1253, 1754, 1874, 1942
BseRI GAGGAG 5 cut(s) 38, 68, 193, 1014, 1339
BseSI GKGCMC 2 cut(s) 133, 594
BseX3I CGGCCG 1 cut(s) 304
Bsh1236I CGCG 4 cut(s) 212, 309, 1298, 2266
Bsh1285I CGRYCG 1 cut(s) 307
BshFI GGCC 8 cut(s) 131, 306, 346, 491, 709, 1165, 1222, 2194
BshNI GGYRCC 1 cut(s) 1963
BsiEI CGRYCG 1 cut(s) 307
BsiHKAI GWGCWC 2 cut(s) 69, 123
BsiHKCI CYCGRG 2 cut(s) 124, 1667
BsiSI CCGG 6 cut(s) 20, 94, 189, 195, 201, 2191
BslFI GGGAC 1 cut(s) 510
BsmAI GTCTC 4 cut(s) 145, 1056, 1747, 2230
BsmBI CGTCTC 1 cut(s) 1747
BsmFI GGGAC 1 cut(s) 510
BsnI GGCC 8 cut(s) 131, 306, 346, 491, 709, 1165, 1222, 2194
Bso31I GGTCTC 1 cut(s) 145
BsoBI CYCGRG 2 cut(s) 124, 1667
Bsp120I GGGCCC 1 cut(s) 129
Bsp1286I GDGCHC 4 cut(s) 69, 123, 133, 594
Bsp13I TCCGGA 1 cut(s) 93
Bsp19I CCATGG 3 cut(s) 427, 1166, 1513
BspANI GGCC 8 cut(s) 131, 306, 346, 491, 709, 1165, 1222, 2194
BspCNI CTCAG 3 cut(s) 811, 1273, 2003
BspEI TCCGGA 1 cut(s) 93
BspFNI CGCG 4 cut(s) 212, 309, 1298, 2266
BspHI TCATGA 1 cut(s) 2103
BspLI GGNNCC 7 cut(s) 131, 193, 199, 205, 504, 1339, 1965
BspMAI CTGCAG 2 cut(s) 264, 2235
BspMI ACCTGC 1 cut(s) 2071
BspOI GCTAGC 2 cut(s) 990, 2190
BspQI GCTCTTC 1 cut(s) 2253
BspT107I GGYRCC 1 cut(s) 1963
BspTNI GGTCTC 1 cut(s) 145
BsrDI GCAATG 2 cut(s) 1987, 2150
BsrFI RCCGGY 1 cut(s) 2190
BsrI ACTGG 6 cut(s) 298, 449, 1253, 1754, 1874, 1942
BssAI RCCGGY 1 cut(s) 2190
BssNAI GTATAC 1 cut(s) 1510
BssSI CACGAG 1 cut(s) 1785
BssT1I CCWWGG 6 cut(s) 427, 570, 1166, 1223, 1513, 2057
Bst1107I GTATAC 1 cut(s) 1510
Bst2BI CACGAG 1 cut(s) 1785
Bst2UI CCWGG 6 cut(s) 44, 494, 530, 1056, 1342, 1558
Bst4CI ACNGT 5 cut(s) 772, 1408, 1507, 1876, 2301
Bst6I CTCTTC 2 cut(s) 218, 2253
BstAPI GCANNNNNTGC 2 cut(s) 409, 808
BstBAI YACGTR 1 cut(s) 1703
BstC8I GCNNGC 6 cut(s) 344, 988, 1381, 2171, 2188, 2192
BstDEI CTNAG 4 cut(s) 111, 798, 1260, 1990
BstDSI CCRYGG 3 cut(s) 427, 1166, 1513
BstENI CCTNNNNNAGG 1 cut(s) 74
BstF5I GGATG 7 cut(s) 845, 973, 1048, 1627, 1824, 1965, 2011
BstFNI CGCG 4 cut(s) 212, 309, 1298, 2266
BstHHI GCGC 2 cut(s) 311, 2266
BstMAI GTCTC 4 cut(s) 145, 1056, 1747, 2230
BstMCI CGRYCG 1 cut(s) 307
BstNI CCWGG 6 cut(s) 44, 494, 530, 1056, 1342, 1558
BstNSI RCATGY 1 cut(s) 1319
BstSCI CCNGG 6 cut(s) 42, 492, 528, 1054, 1340, 1556
BstSFI CTRYAG 4 cut(s) 260, 2094, 2231, 2300
BstSLI GKGCMC 2 cut(s) 133, 594
BstUI CGCG 4 cut(s) 212, 309, 1298, 2266
BstV2I GAAGAC 2 cut(s) 1467, 1836
BstX2I RGATCY 8 cut(s) 39, 502, 523, 1337, 1834, 2052, 2121, 2227
BstYI RGATCY 8 cut(s) 39, 502, 523, 1337, 1834, 2052, 2121, 2227
BstZ17I GTATAC 1 cut(s) 1510
BstZI CGGCCG 1 cut(s) 304
BsuRI GGCC 8 cut(s) 131, 306, 346, 491, 709, 1165, 1222, 2194
BtgI CCRYGG 3 cut(s) 427, 1166, 1513
BtgZI GCGATG 1 cut(s) 1095
BtrI CACGTC 3 cut(s) 1423, 1760, 1904
BtsCI GGATG 7 cut(s) 845, 973, 1048, 1627, 1824, 1965, 2011
BtsI GCAGTG 1 cut(s) 1381
BtsIMutI CAGTG 2 cut(s) 1381, 1428
BveI ACCTGC 1 cut(s) 2071
Cac8I GCNNGC 6 cut(s) 344, 988, 1381, 2171, 2188, 2192
CaiI CAGNNNCTG 4 cut(s) 49, 379, 808, 1386
CciI TCATGA 1 cut(s) 2103
CciNI GCGGCCGC 1 cut(s) 304
CfoI GCGC 2 cut(s) 311, 2266
Cfr10I RCCGGY 1 cut(s) 2190
Cfr13I GGNCC 8 cut(s) 129, 130, 153, 344, 490, 532, 1344, 2268
CpoI CGGWCCG 1 cut(s) 2268
CseI GACGC 3 cut(s) 218, 736, 1661
Csp6I GTAC 3 cut(s) 759, 1404, 1525
CspI CGGWCCG 1 cut(s) 2268
CviQI GTAC 3 cut(s) 759, 1404, 1525
DdeI CTNAG 4 cut(s) 111, 798, 1260, 1990
DraI TTTAAA 1 cut(s) 1281
EaeI YGGCCR 3 cut(s) 304, 1163, 2192
EagI CGGCCG 1 cut(s) 304
Eam1104I CTCTTC 2 cut(s) 218, 2253
EarI CTCTTC 2 cut(s) 218, 2253
EciI GGCGGA 1 cut(s) 2260
EclXI CGGCCG 1 cut(s) 304
Eco130I CCWWGG 6 cut(s) 427, 570, 1166, 1223, 1513, 2057
Eco24I GRGCYC 1 cut(s) 133
Eco31I GGTCTC 1 cut(s) 145
Eco47I GGWCC 4 cut(s) 153, 532, 1344, 2268
Eco52I CGGCCG 1 cut(s) 304
Eco57I CTGAAG 7 cut(s) 53, 92, 480, 736, 1317, 1576, 1859
Eco72I CACGTG 1 cut(s) 1703
Eco88I CYCGRG 2 cut(s) 124, 1667
EcoNI CCTNNNNNAGG 1 cut(s) 74
EcoO109I RGGNCCY 2 cut(s) 129, 490
EcoRII CCWGG 6 cut(s) 42, 492, 528, 1054, 1340, 1556
EcoT14I CCWWGG 6 cut(s) 427, 570, 1166, 1223, 1513, 2057
EcoT38I GRGCYC 1 cut(s) 133
ErhI CCWWGG 6 cut(s) 427, 570, 1166, 1223, 1513, 2057
Esp3I CGTCTC 1 cut(s) 1747
FaqI GGGAC 1 cut(s) 510
FauI CCCGC 2 cut(s) 335, 2148
FbaI TGATCA 2 cut(s) 937, 1237
FblI GTMKAC 3 cut(s) 233, 1419, 1509
FokI GGATG 7 cut(s) 832, 980, 1055, 1614, 1811, 1972, 2018
FriOI GRGCYC 1 cut(s) 133
FspBI CTAG 6 cut(s) 987, 996, 1745, 1856, 2117, 2187
GlaI GCGC 2 cut(s) 310, 2265
GsuI CTGGAG 4 cut(s) 71, 1038, 1540, 1771
HaeIII GGCC 8 cut(s) 131, 306, 346, 491, 709, 1165, 1222, 2194
HapII CCGG 6 cut(s) 20, 94, 189, 195, 201, 2191
HgaI GACGC 3 cut(s) 218, 736, 1661
HhaI GCGC 2 cut(s) 311, 2266
Hin6I GCGC 2 cut(s) 309, 2264
HinP1I GCGC 2 cut(s) 309, 2264
HincII GTYRAC 1 cut(s) 1363
HindII GTYRAC 1 cut(s) 1363
HindIII AAGCTT 2 cut(s) 748, 2219
HinfI GANTC 5 cut(s) 159, 183, 564, 743, 1073
HpaI GTTAAC 1 cut(s) 1363
HpaII CCGG 6 cut(s) 20, 94, 189, 195, 201, 2191
HphI GGTGA 4 cut(s) 367, 559, 1898, 2095
Hpy166II GTNNAC 6 cut(s) 234, 358, 1363, 1420, 1426, 1510
Hpy8I GTNNAC 6 cut(s) 234, 358, 1363, 1420, 1426, 1510
Hpy99I CGWCG 4 cut(s) 212, 317, 352, 1761
HpyAV CCTTC 6 cut(s) 902, 1074, 1085, 1144, 1181, 1600
HpyCH4III ACNGT 5 cut(s) 772, 1408, 1507, 1876, 2301
HpyCH4IV ACGT 4 cut(s) 1422, 1702, 1759, 1903
HpyF3I CTNAG 4 cut(s) 111, 798, 1260, 1990
HpySE526I ACGT 4 cut(s) 1422, 1702, 1759, 1903
HspAI GCGC 2 cut(s) 309, 2264
Kpn2I TCCGGA 1 cut(s) 93
KroI GCCGGC 1 cut(s) 2190
KroNI GCCGGC 1 cut(s) 2192
Ksp22I TGATCA 2 cut(s) 937, 1237
KspAI GTTAAC 1 cut(s) 1363
LguI GCTCTTC 1 cut(s) 2253
LmnI GCTCC 9 cut(s) 25, 64, 96, 191, 197, 203, 1652, 2035, 2090
LweI GCATC 5 cut(s) 97, 833, 854, 958, 1689
MaeI CTAG 6 cut(s) 987, 996, 1745, 1856, 2117, 2187
MaeII ACGT 4 cut(s) 1422, 1702, 1759, 1903
MaeIII GTNAC 6 cut(s) 565, 772, 1657, 1684, 1719, 2041
MboII GAAGA 9 cut(s) 46, 49, 235, 1276, 1472, 1586, 1793, 1836, 2240
MfeI CAATTG 1 cut(s) 819
MflI RGATCY 8 cut(s) 39, 502, 523, 1337, 1834, 2052, 2121, 2227
MhlI GDGCHC 4 cut(s) 69, 123, 133, 594
MlsI TGGCCA 1 cut(s) 1165
MluNI TGGCCA 1 cut(s) 1165
MlyI GAGTC 2 cut(s) 192, 573
MmeI TCCRAC 3 cut(s) 200, 314, 1983
Mox20I TGGCCA 1 cut(s) 1165
MroI TCCGGA 1 cut(s) 93
MroNI GCCGGC 1 cut(s) 2190
MroXI GAANNNNTTC 3 cut(s) 1063, 1920, 2220
MscI TGGCCA 1 cut(s) 1165
MseI TTAA 6 cut(s) 518, 1200, 1280, 1356, 1362, 2156
MslI CAYNNNNRTG 1 cut(s) 1632
Msp20I TGGCCA 1 cut(s) 1165
MspA1I CMGCKG 6 cut(s) 151, 259, 303, 458, 482, 1386
MspI CCGG 6 cut(s) 20, 94, 189, 195, 201, 2191
MspR9I CCNGG 6 cut(s) 44, 494, 530, 1056, 1342, 1558
MunI CAATTG 1 cut(s) 819
MvaI CCWGG 6 cut(s) 44, 494, 530, 1056, 1342, 1558
MvnI CGCG 4 cut(s) 212, 309, 1298, 2266
NaeI GCCGGC 1 cut(s) 2192
NcoI CCATGG 3 cut(s) 427, 1166, 1513
NgoMIV GCCGGC 1 cut(s) 2190
NheI GCTAGC 2 cut(s) 986, 2186
NlaIV GGNNCC 7 cut(s) 131, 193, 199, 205, 504, 1339, 1965
NmuCI GTSAC 2 cut(s) 565, 1657
NotI GCGGCCGC 1 cut(s) 304
NspI RCATGY 1 cut(s) 1319
PagI TCATGA 1 cut(s) 2103
PaqCI CACCTGC 1 cut(s) 2071
PasI CCCWGGG 1 cut(s) 493
PciSI GCTCTTC 1 cut(s) 2253
PdiI GCCGGC 1 cut(s) 2192
PdmI GAANNNNTTC 3 cut(s) 1063, 1920, 2220
PfeI GAWTC 3 cut(s) 159, 743, 1073
PflMI CCANNNNNTGG 2 cut(s) 49, 1960
PfoI TCCNGGA 2 cut(s) 1054, 1340
PleI GAGTC 2 cut(s) 191, 572
PmaCI CACGTG 1 cut(s) 1703
PmlI CACGTG 1 cut(s) 1703
PpsI GAGTC 2 cut(s) 191, 572
Ppu21I YACGTR 1 cut(s) 1703
Psp6I CCWGG 6 cut(s) 42, 492, 528, 1054, 1340, 1556
PspCI CACGTG 1 cut(s) 1703
PspGI CCWGG 6 cut(s) 42, 492, 528, 1054, 1340, 1556
PspN4I GGNNCC 7 cut(s) 131, 193, 199, 205, 504, 1339, 1965
PspOMI GGGCCC 1 cut(s) 129
PspPI GGNCC 8 cut(s) 129, 130, 153, 344, 490, 532, 1344, 2268
PstI CTGCAG 2 cut(s) 264, 2235
PstNI CAGNNNCTG 4 cut(s) 49, 379, 808, 1386
PsuI RGATCY 8 cut(s) 39, 502, 523, 1337, 1834, 2052, 2121, 2227
PvuII CAGCTG 2 cut(s) 458, 1386
RsaI GTAC 3 cut(s) 760, 1405, 1526
RsaNI GTAC 3 cut(s) 759, 1404, 1525
RseI CAYNNNNRTG 1 cut(s) 1632
Rsr2I CGGWCCG 1 cut(s) 2268
RsrII CGGWCCG 1 cut(s) 2268
SapI GCTCTTC 1 cut(s) 2253
SaqAI TTAA 6 cut(s) 518, 1200, 1280, 1356, 1362, 2156
Sau96I GGNCC 8 cut(s) 129, 130, 153, 344, 490, 532, 1344, 2268
ScaI AGTACT 1 cut(s) 1405
SchI GAGTC 2 cut(s) 192, 573
ScrFI CCNGG 6 cut(s) 44, 494, 530, 1056, 1342, 1558
SduI GDGCHC 4 cut(s) 69, 123, 133, 594
SfaNI GCATC 5 cut(s) 97, 833, 854, 958, 1689
SfcI CTRYAG 4 cut(s) 260, 2094, 2231, 2300
SinI GGWCC 4 cut(s) 153, 532, 1344, 2268
SmiMI CAYNNNNRTG 1 cut(s) 1632
SspI AATATT 1 cut(s) 1568
SspMI CTAG 6 cut(s) 987, 996, 1745, 1856, 2117, 2187
StyD4I CCNGG 6 cut(s) 42, 492, 528, 1054, 1340, 1556
StyI CCWWGG 6 cut(s) 427, 570, 1166, 1223, 1513, 2057
TaaI ACNGT 5 cut(s) 772, 1408, 1507, 1876, 2301
TaiI ACGT 4 cut(s) 1425, 1705, 1762, 1906
TaqI TCGA 6 cut(s) 315, 872, 1312, 1624, 1831, 2224
TaqII GACCGA 1 cut(s) 127
TatI WGTACW 1 cut(s) 1403
TfiI GAWTC 3 cut(s) 159, 743, 1073
Tru1I TTAA 6 cut(s) 518, 1200, 1280, 1356, 1362, 2156
Tru9I TTAA 6 cut(s) 518, 1200, 1280, 1356, 1362, 2156
TscAI CASTG 2 cut(s) 1381, 1435
TseFI GTSAC 2 cut(s) 565, 1657
Tsp45I GTSAC 2 cut(s) 565, 1657
TspGWI ACGGA 2 cut(s) 1542, 2034
TspRI CASTG 2 cut(s) 1381, 1435
Van91I CCANNNNNTGG 2 cut(s) 49, 1960
VpaK11BI GGWCC 4 cut(s) 153, 532, 1344, 2268
XagI CCTNNNNNAGG 1 cut(s) 74
XapI RAATTY 3 cut(s) 838, 876, 973
XceI RCATGY 1 cut(s) 1319
XmiI GTMKAC 3 cut(s) 233, 1419, 1509
XmnI GAANNNNTTC 3 cut(s) 1063, 1920, 2220
XspI CTAG 6 cut(s) 987, 996, 1745, 1856, 2117, 2187
ZrmI AGTACT 1 cut(s) 1405
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.