AT3G09405

Hydrolyzes acetyl esters in homogalacturonan regions of pectin. In type I primary cell wall, galacturonic acid residues of pectin can be acetylated at the O-2 and O-3 positions. Decreasing the degree of acetylation of pectin gels in vitro alters their physical properties

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0001587
Physical Location & Seq
Reverse (-)
2894798 .. 2897674
2877 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G09405.1

Sequence Viewer

Length: 1230 bp
ATGGTGATTCGCTCTCTGCTACAATGCAGAACATGGTCTAAGAGTGACTGGCTTCTCGCTTCAATCGGAATAGTCTTGATCGTTTATTCCTTTTCTTTGTCCTTCAATTCAACTTCAGACTCTATCCCCTCCGTTGACCGTAGCGATCTAGTTAAACTGAAGCTGTCGAGTAAAGCTAAAGAGAGAGGAGCTTTTTGCTTAGATGGAAGCTTACCTGGTTATCACTTTCATAAGGGTTCAGGATCTGGCTCCAATAGCTGGCTTCTTTATTTAGAGGGTGGAGGCGGGTGCCGTACAATTGAGTCATGTTCTGCTAGAGCAATGACTAGGTTAGGTTCTTCCAACTTCTTTGAACATGAAGTGCCTTTTTTTGGCGTTTTAAGCAGTGACCCATCTCAAAACCCTGACTTCTTTAACTGGAACAGAGTTATGATACGGTACTGTGATGGTGCTTGTTTTTCTGGACACCCTGAAGCTGAATTCAAGAATGAAACACGGCTTTTCTTCCGCGGCCAGCTCATTTGGGAGGCTATCATGGATGAATTGTTGTCGATGGGCATGTCACATGCGAAACGTGCCATGCTCACAGGATGTTCTGCTGGTGGCTTGTCTACTCTAATACATTGTGACTACTTTCGAGACCATCTGCCTAAAGATGCAACTGTTAAATGTGTTTCCGATGGAGGCTATATTCTTAATGTGCTTGATGTCCTTGGAAACCCAACAATGGGATCTTTCTTTCATGATGTTGTTACCCTGCAGAGCGTAGATAAGAGCTTGGATCAGAATTGTGTTGCCAAAATGGAACCATCTAAGTGTATGTTTCCTCAAGAATCTCTTAAAAACATAAGAACTCCGGTATTTCTTGTCAACACAGCCTACGATTATTGGCAGATTCAAAACGGCCTGGTACCAGATTCTCCGGATCTAGATGAAAGATGGAAAATTTGTAGACTCAATATTCAGGAATGTGATGCCGCACAAATGAAAGTTCTGCATGGTTTCCGCAGTTCCCTGATCGATGCGATAGGAGAATTTCATGTGAACAAAGAGGGTGGGATGTTTATAAATTCGTGCAATTCTCATTGCCAGATAAGAGAGTCATGGCATTCCGCAACCTCAACAAGAATCGAAAATAAGACGATTGCAGAGTCTGTAGGTGATTGGTACTTCAACCGAAAACCGGTGAAGCTAATCGATTGTCCTTATCCGTGCAATGCCTCTTGTTGA

Protein Analysis

409

Amino Acids

45.75

Weight (kDa)

6.31

Isoelectric Point (pI)

41.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PAE PF03283 48 - 409 1.1e-146 Pectinacetylesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1067
Acc65I GGTACC 1 cut(s) 910
AccB1I GGYRCC 2 cut(s) 288, 910
AccB7I CCANNNNNTGG 1 cut(s) 258
AccI GTMKAC 2 cut(s) 611, 952
AccII CGCG 1 cut(s) 510
AccIII TCCGGA 1 cut(s) 922
AciI CCGC 6 cut(s) 285, 508, 510, 978, 1006, 1113
AclWI GGATC 4 cut(s) 250, 739, 789, 933
AcoI YGGCCR 1 cut(s) 511
AcsI RAATTY 4 cut(s) 479, 945, 1034, 1069
AcuI CTGAAG 3 cut(s) 99, 179, 492
AfaI GTAC 4 cut(s) 295, 440, 912, 1169
AfiI CCNNNNNNNGG 5 cut(s) 258, 371, 727, 728, 1183
AgeI ACCGGT 1 cut(s) 1183
AgsI TTSAA 7 cut(s) 63, 106, 111, 353, 484, 899, 1174
AjnI CCWGG 2 cut(s) 214, 906
AluBI AGCT 9 cut(s) 163, 176, 191, 210, 258, 476, 517, 777, 1192
AluI AGCT 9 cut(s) 163, 176, 191, 210, 258, 476, 517, 777, 1192
Alw26I GTCTC 1 cut(s) 633
AlwI GGATC 4 cut(s) 250, 739, 789, 933
AlwNI CAGNNNCTG 2 cut(s) 245, 1154
Aor13HI TCCGGA 1 cut(s) 922
AoxI GGCC 2 cut(s) 511, 904
ApoI RAATTY 4 cut(s) 479, 945, 1034, 1069
AsiGI ACCGGT 1 cut(s) 1183
Asp718I GGTACC 1 cut(s) 910
AsuHPI GGTGA 3 cut(s) 16, 1172, 1198
BanI GGYRCC 2 cut(s) 288, 910
BccI CCATC 8 cut(s) 197, 400, 440, 547, 651, 674, 817, 933
BceAI ACGGC 3 cut(s) 276, 512, 919
BciT130I CCWGG 2 cut(s) 216, 908
BcoDI GTCTC 1 cut(s) 633
BfaI CTAG 4 cut(s) 149, 315, 327, 929
BfmI CTRYAG 2 cut(s) 758, 1155
BisI GCNGC 2 cut(s) 511, 978
BlsI GCNGC 2 cut(s) 512, 979
Bme1390I CCNGG 2 cut(s) 216, 908
BmiI GGNNCC 4 cut(s) 250, 290, 807, 912
BmrFI CCNGG 2 cut(s) 216, 908
BmsI GCATC 3 cut(s) 646, 964, 1012
BpuEI CTTGAG 1 cut(s) 813
Bsa29I ATCGAT 2 cut(s) 1020, 1197
BsaI GGTCTC 1 cut(s) 633
BsaJI CCNNGG 2 cut(s) 508, 712
BsaWI WCCGGW 3 cut(s) 856, 922, 1183
Bsc4I CCNNNNNNNGG 5 cut(s) 258, 371, 727, 728, 1183
Bse118I RCCGGY 1 cut(s) 1183
Bse1I ACTGG 2 cut(s) 53, 422
Bse3DI GCAATG 3 cut(s) 327, 1084, 1222
BseAI TCCGGA 1 cut(s) 922
BseBI CCWGG 2 cut(s) 216, 908
BseCI ATCGAT 2 cut(s) 1020, 1197
BseDI CCNNGG 2 cut(s) 508, 712
BseGI GGATG 3 cut(s) 544, 596, 1065
BseLI CCNNNNNNNGG 5 cut(s) 258, 371, 727, 728, 1183
BseMI GCAATG 3 cut(s) 327, 1084, 1222
BseNI ACTGG 2 cut(s) 53, 422
BseRI GAGGAG 1 cut(s) 201
Bsh1236I CGCG 1 cut(s) 510
BshFI GGCC 2 cut(s) 513, 906
BshNI GGYRCC 2 cut(s) 288, 910
BshTI ACCGGT 1 cut(s) 1183
BshVI ATCGAT 2 cut(s) 1020, 1197
BsiSI CCGG 3 cut(s) 857, 923, 1184
BslI CCNNNNNNNGG 5 cut(s) 258, 371, 727, 728, 1183
BsmAI GTCTC 1 cut(s) 633
BsmI GAATGC 1 cut(s) 1108
BsnI GGCC 2 cut(s) 513, 906
Bso31I GGTCTC 1 cut(s) 633
Bsp13I TCCGGA 1 cut(s) 922
Bsp143I GATC 7 cut(s) 78, 145, 242, 731, 781, 925, 1017
BspACI CCGC 6 cut(s) 285, 508, 510, 978, 1006, 1113
BspANI GGCC 2 cut(s) 513, 906
BspDI ATCGAT 2 cut(s) 1020, 1197
BspEI TCCGGA 1 cut(s) 922
BspFNI CGCG 1 cut(s) 510
BspHI TCATGA 1 cut(s) 742
BspLI GGNNCC 4 cut(s) 250, 290, 807, 912
BspMAI CTGCAG 1 cut(s) 762
BspPI GGATC 4 cut(s) 250, 739, 789, 933
BspT107I GGYRCC 2 cut(s) 288, 910
BspTNI GGTCTC 1 cut(s) 633
BsrDI GCAATG 3 cut(s) 327, 1084, 1222
BsrFI RCCGGY 1 cut(s) 1183
BsrI ACTGG 2 cut(s) 53, 422
BssAI RCCGGY 1 cut(s) 1183
BssECI CCNNGG 2 cut(s) 508, 712
BssMI GATC 7 cut(s) 78, 145, 242, 731, 781, 925, 1017
BssT1I CCWWGG 1 cut(s) 712
Bst2UI CCWGG 2 cut(s) 216, 908
Bst4CI ACNGT 4 cut(s) 140, 438, 443, 664
BstC8I GCNNGC 2 cut(s) 260, 515
BstDEI CTNAG 3 cut(s) 39, 199, 813
BstDSI CCRYGG 1 cut(s) 508
BstF5I GGATG 3 cut(s) 544, 596, 1065
BstFNI CGCG 1 cut(s) 510
BstKTI GATC 7 cut(s) 81, 148, 245, 734, 784, 928, 1020
BstMAI GTCTC 1 cut(s) 633
BstMBI GATC 7 cut(s) 78, 145, 242, 731, 781, 925, 1017
BstMWI GCNNNNNNNGC 3 cut(s) 255, 381, 575
BstNI CCWGG 2 cut(s) 216, 908
BstNSI RCATGY 2 cut(s) 562, 569
BstSCI CCNGG 2 cut(s) 214, 906
BstSFI CTRYAG 2 cut(s) 758, 1155
BstUI CGCG 1 cut(s) 510
BstX2I RGATCY 3 cut(s) 242, 731, 925
BstYI RGATCY 3 cut(s) 242, 731, 925
Bsu15I ATCGAT 2 cut(s) 1020, 1197
BsuRI GGCC 2 cut(s) 513, 906
BsuTUI ATCGAT 2 cut(s) 1020, 1197
BtgI CCRYGG 1 cut(s) 508
BtsCI GGATG 3 cut(s) 544, 596, 1065
BtsI GCAGTG 1 cut(s) 391
BtsIMutI CAGTG 1 cut(s) 391
Cac8I GCNNGC 2 cut(s) 260, 515
CaiI CAGNNNCTG 2 cut(s) 245, 1154
CciI TCATGA 1 cut(s) 742
Cfr10I RCCGGY 1 cut(s) 1183
Cfr42I CCGCGG 1 cut(s) 511
ClaI ATCGAT 2 cut(s) 1020, 1197
CsiI ACCWGGT 1 cut(s) 214
Csp6I GTAC 4 cut(s) 294, 439, 911, 1168
CspAI ACCGGT 1 cut(s) 1183
CspCI CAANNNNNGTGG 2 cut(s) 1036, 1071
CviQI GTAC 4 cut(s) 294, 439, 911, 1168
DdeI CTNAG 3 cut(s) 39, 199, 813
DpnI GATC 7 cut(s) 80, 147, 244, 733, 783, 927, 1019
DpnII GATC 7 cut(s) 78, 145, 242, 731, 781, 925, 1017
EaeI YGGCCR 1 cut(s) 511
Eco130I CCWWGG 1 cut(s) 712
Eco31I GGTCTC 1 cut(s) 633
Eco57I CTGAAG 3 cut(s) 99, 179, 492
EcoRI GAATTC 1 cut(s) 479
EcoRII CCWGG 2 cut(s) 214, 906
EcoT14I CCWWGG 1 cut(s) 712
ErhI CCWWGG 1 cut(s) 712
FalI AAGNNNNNCTT 2 cut(s) 822, 854
FauI CCCGC 1 cut(s) 278
FblI GTMKAC 2 cut(s) 611, 952
Fnu4HI GCNGC 2 cut(s) 511, 978
FokI GGATG 3 cut(s) 551, 603, 1072
Fsp4HI GCNGC 2 cut(s) 511, 978
FspBI CTAG 4 cut(s) 149, 315, 327, 929
GluI GCNGC 2 cut(s) 511, 978
HaeIII GGCC 2 cut(s) 513, 906
HapII CCGG 3 cut(s) 857, 923, 1184
HincII GTYRAC 2 cut(s) 136, 871
HindII GTYRAC 2 cut(s) 136, 871
HindIII AAGCTT 1 cut(s) 208
HpaII CCGG 3 cut(s) 857, 923, 1184
HphI GGTGA 3 cut(s) 16, 1172, 1198
Hpy166II GTNNAC 5 cut(s) 136, 612, 871, 953, 1045
Hpy188I TCNGA 4 cut(s) 68, 118, 679, 786
Hpy8I GTNNAC 5 cut(s) 136, 612, 871, 953, 1045
HpyAV CCTTC 1 cut(s) 112
HpyCH4III ACNGT 4 cut(s) 140, 438, 443, 664
HpyCH4IV ACGT 1 cut(s) 574
HpyCH4V TGCA 7 cut(s) 27, 659, 760, 997, 1077, 1148, 1215
HpyF10VI GCNNNNNNNGC 3 cut(s) 255, 381, 575
HpyF3I CTNAG 3 cut(s) 39, 199, 813
HpySE526I ACGT 1 cut(s) 574
Kpn2I TCCGGA 1 cut(s) 922
KpnI GGTACC 1 cut(s) 914
KspI CCGCGG 1 cut(s) 511
Kzo9I GATC 7 cut(s) 78, 145, 242, 731, 781, 925, 1017
LmnI GCTCC 2 cut(s) 188, 254
LweI GCATC 3 cut(s) 646, 964, 1012
MabI ACCWGGT 1 cut(s) 214
MaeI CTAG 4 cut(s) 149, 315, 327, 929
MaeII ACGT 1 cut(s) 574
MaeIII GTNAC 5 cut(s) 44, 386, 561, 626, 751
MalI GATC 7 cut(s) 80, 147, 244, 733, 783, 927, 1019
MboI GATC 7 cut(s) 78, 145, 242, 731, 781, 925, 1017
MboII GAAGA 2 cut(s) 330, 496
MfeI CAATTG 1 cut(s) 297
MflI RGATCY 3 cut(s) 242, 731, 925
MluCI AATT 9 cut(s) 106, 297, 479, 542, 787, 945, 1034, 1069, 1078
MlyI GAGTC 5 cut(s) 113, 311, 948, 1109, 1160
MmeI TCCRAC 1 cut(s) 366
MnlI CCTC 9 cut(s) 139, 179, 268, 275, 520, 677, 837, 1045, 1129
MroI TCCGGA 1 cut(s) 922
MseI TTAA 6 cut(s) 153, 380, 414, 666, 696, 840
MslI CAYNNNNRTG 1 cut(s) 814
MspA1I CMGCKG 1 cut(s) 510
MspI CCGG 3 cut(s) 857, 923, 1184
MspR9I CCNGG 2 cut(s) 216, 908
MunI CAATTG 1 cut(s) 297
Mva1269I GAATGC 1 cut(s) 1108
MvaI CCWGG 2 cut(s) 216, 908
MvnI CGCG 1 cut(s) 510
MwoI GCNNNNNNNGC 3 cut(s) 255, 381, 575
NdeII GATC 7 cut(s) 78, 145, 242, 731, 781, 925, 1017
NlaIV GGNNCC 4 cut(s) 250, 290, 807, 912
NmuCI GTSAC 4 cut(s) 44, 386, 561, 626
NspI RCATGY 2 cut(s) 562, 569
PagI TCATGA 1 cut(s) 742
PctI GAATGC 1 cut(s) 1108
PfeI GAWTC 5 cut(s) 7, 833, 895, 917, 1128
PflMI CCANNNNNTGG 1 cut(s) 258
PinAI ACCGGT 1 cut(s) 1183
PkrI GCNGC 2 cut(s) 512, 979
PleI GAGTC 5 cut(s) 113, 310, 948, 1108, 1159
PpsI GAGTC 5 cut(s) 113, 310, 948, 1108, 1159
PsiI TTATAA 1 cut(s) 1067
Psp6I CCWGG 2 cut(s) 214, 906
PspGI CCWGG 2 cut(s) 214, 906
PspN4I GGNNCC 4 cut(s) 250, 290, 807, 912
PstI CTGCAG 1 cut(s) 762
PstNI CAGNNNCTG 2 cut(s) 245, 1154
PsuI RGATCY 3 cut(s) 242, 731, 925
RsaI GTAC 4 cut(s) 295, 440, 912, 1169
RsaNI GTAC 4 cut(s) 294, 439, 911, 1168
RseI CAYNNNNRTG 1 cut(s) 814
SacII CCGCGG 1 cut(s) 511
SaqAI TTAA 6 cut(s) 153, 380, 414, 666, 696, 840
SatI GCNGC 2 cut(s) 511, 978
Sau3AI GATC 7 cut(s) 78, 145, 242, 731, 781, 925, 1017
SchI GAGTC 5 cut(s) 113, 311, 948, 1109, 1160
ScrFI CCNGG 2 cut(s) 216, 908
SexAI ACCWGGT 1 cut(s) 214
SfaNI GCATC 3 cut(s) 646, 964, 1012
SfcI CTRYAG 2 cut(s) 758, 1155
Sfr303I CCGCGG 1 cut(s) 511
SgrBI CCGCGG 1 cut(s) 511
SmiMI CAYNNNNRTG 1 cut(s) 814
SmlI CTYRAG 1 cut(s) 828
SmoI CTYRAG 1 cut(s) 828
Sse9I AATT 9 cut(s) 106, 297, 479, 542, 787, 945, 1034, 1069, 1078
SsiI CCGC 6 cut(s) 285, 508, 510, 978, 1006, 1113
SspI AATATT 1 cut(s) 961
SspMI CTAG 4 cut(s) 149, 315, 327, 929
StyD4I CCNGG 2 cut(s) 214, 906
StyI CCWWGG 1 cut(s) 712
TaaI ACNGT 4 cut(s) 140, 438, 443, 664
TaiI ACGT 1 cut(s) 577
TaqI TCGA 6 cut(s) 167, 551, 637, 1020, 1131, 1197
TasI AATT 9 cut(s) 106, 297, 479, 542, 787, 945, 1034, 1069, 1078
TauI GCSGC 2 cut(s) 513, 980
TfiI GAWTC 5 cut(s) 7, 833, 895, 917, 1128
Tru1I TTAA 6 cut(s) 153, 380, 414, 666, 696, 840
Tru9I TTAA 6 cut(s) 153, 380, 414, 666, 696, 840
TscAI CASTG 1 cut(s) 391
TseFI GTSAC 4 cut(s) 44, 386, 561, 626
Tsp45I GTSAC 4 cut(s) 44, 386, 561, 626
TspDTI ATGAA 8 cut(s) 218, 372, 504, 555, 731, 948, 1001, 1028
TspGWI ACGGA 2 cut(s) 121, 1200
TspRI CASTG 1 cut(s) 391
Van91I CCANNNNNTGG 1 cut(s) 258
XapI RAATTY 4 cut(s) 479, 945, 1034, 1069
XbaI TCTAGA 1 cut(s) 928
XceI RCATGY 2 cut(s) 562, 569
XmiI GTMKAC 2 cut(s) 611, 952
XspI CTAG 4 cut(s) 149, 315, 327, 929
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.