Rh3CG151500

Hydrolyzes acetyl esters in homogalacturonan regions of pectin. In type I primary cell wall, galacturonic acid residues of pectin can be acetylated at the O-2 and O-3 positions. Decreasing the degree of acetylation of pectin gels in vitro alters their physical properties

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3C
OG0001587
Physical Location & Seq
Reverse (-)
12675047 .. 12678026
2980 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3CG151500.1

Sequence Viewer

Length: 1179 bp
ATGGCGGGGTCAACGGCGAACCTCAGACTCCGCAGCCTTGTATGGTGGAGAAAGGACTGGGCAATCGCCACCCTCGCCTTCACCGTCATCGTCTTCCTTCTCACTCTCATCTCCAACTCTTTGCGCGACGAACCAGACTCCGGCGTCACCAACCCCTTCCATCCAACCGCCGCCGCCGATTTGGTCGACTTGACTCTGCTGCACGACGCCGAACGCCGAGGCGCCCTTTGCTTAGATGGGAGTGCTCCCGGGTACCATTTCCGAAAGGGGTTCGGATCCGGAGCCAAAAATTGGTTGCTTCACATTGAGGGTGGAGGTTGGTGCAATACAATAGAGTCATGTTCTTTTCGGAAAAGGACTCCATTAGGGTCTTCCAAGTACATGGATCGTAGGGTTCAGTTTTCCGGGATTTTGAGCTCTCAGCCGTCAGAAAATCCTGACTTCTTTAACTGGAACAAAGTCAAAATACGGTACTGTGATGGCGCGTCTTTTGCTGGCCACCCCGAAAATGAGCCCAAGAATGGATCAAAGCTTTACTTTAGGGGACAGCTCATCTGGGAAGCACTTATGGATGAACTCTTGTCAATTGGCTTGTCAAAGGCGAAACAGGCACTTCTTTCTGGATGCTCTGCCGGTGGGTTGGCAACTCTTATACACTGTGATGACTTTCGAGGTCTTTTACCTAGGGATTCAACTGTCAAATGTCTTGCTGATGCAGGTTTTTTTCTCGATGAGTTATGCTCTAATCTTACTGCCTTGTGTAATCTTCACTGGTACTCCGTTGATTTAGCAAAAAGTTTGCACAAGGATTGTGTTGCAAGAAAGGAACCAGCTAAGTGTCTCTTTCCCGAAGAAATTATTAAACACGTTAGCACTCCAGTGTTCCTTGTCAACCCAGCTTATGATTTTTGGCAGATACAACATATCTTGATACCTGAAGCATCAGATCCGCATGGCTACTGGAAAAAGTGCAAGCTGAACATCTATAATTGCAATCCTAGCCAGATTGAAATACTGCAAGGTTTCCGTCGTTCTATGCTAAATGCACTGAGCGAATTCCAGAAAAATAAGGAGGGAGGAACTTTTATAAATTCATGTTTTATTCATTGCCAGACATGGTTTTCCGAGACATGGCATTCACCCAGTTCTCCACGAATAAACAATAAGGTGAGGATCTAG

Protein Analysis

392

Amino Acids

44.04

Weight (kDa)

8.44

Isoelectric Point (pI)

46.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PAE PF03283 59 - 389 3.2e-140 Pectinacetylesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1088
AasI GACNNNNNNGTC 1 cut(s) 143
Acc36I ACCTGC 1 cut(s) 707
Acc65I GGTACC 1 cut(s) 252
AccB1I GGYRCC 2 cut(s) 221, 252
AccB7I CCANNNNNTGG 1 cut(s) 291
AccI GTMKAC 1 cut(s) 186
AccII CGCG 2 cut(s) 126, 485
AccIII TCCGGA 1 cut(s) 278
AciI CCGC 6 cut(s) 5, 31, 168, 171, 174, 950
AclWI GGATC 5 cut(s) 270, 283, 393, 532, 941
AcoI YGGCCR 1 cut(s) 496
AcsI RAATTY 2 cut(s) 1055, 1090
AcuI CTGAAG 1 cut(s) 957
AcyI GRCGYC 3 cut(s) 144, 207, 222
AfaI GTAC 4 cut(s) 254, 380, 473, 776
AfiI CCNNNNNNNGG 4 cut(s) 140, 291, 521, 1131
AflIII ACRYGT 1 cut(s) 865
AgsI TTSAA 2 cut(s) 693, 1010
AleI CACNNNNGTG 1 cut(s) 878
AluBI AGCT 6 cut(s) 417, 532, 550, 833, 899, 976
AluI AGCT 6 cut(s) 417, 532, 550, 833, 899, 976
Alw21I GWGCWC 2 cut(s) 247, 419
Alw26I GTCTC 2 cut(s) 845, 1121
AlwI GGATC 5 cut(s) 270, 283, 393, 532, 941
Ama87I CYCGRG 1 cut(s) 248
Aor13HI TCCGGA 1 cut(s) 278
AoxI GGCC 1 cut(s) 496
ApeKI GCWGC 2 cut(s) 33, 199
ApoI RAATTY 2 cut(s) 1055, 1090
Asp718I GGTACC 1 cut(s) 252
AspA2I CCTAGG 1 cut(s) 683
AspLEI GCGC 3 cut(s) 126, 224, 485
AsuC2I CCSGG 3 cut(s) 249, 250, 406
AsuHPI GGTGA 3 cut(s) 73, 139, 1131
AvaI CYCGRG 1 cut(s) 248
AvrII CCTAGG 1 cut(s) 683
BalI TGGCCA 1 cut(s) 498
BamHI GGATCC 1 cut(s) 275
BanI GGYRCC 2 cut(s) 221, 252
BanII GRGCYC 2 cut(s) 419, 516
BbsI GAAGAC 2 cut(s) 85, 363
Bbv12I GWGCWC 2 cut(s) 247, 419
BbvI GCAGC 2 cut(s) 45, 186
BccI CCATC 3 cut(s) 168, 230, 473
BceAI ACGGC 2 cut(s) 30, 409
BcnI CCSGG 3 cut(s) 249, 250, 406
BcoDI GTCTC 2 cut(s) 845, 1121
BfaI CTAG 3 cut(s) 684, 999, 1177
BfoI RGCGCY 1 cut(s) 225
BfuAI ACCTGC 1 cut(s) 707
BisI GCNGC 4 cut(s) 34, 171, 174, 200
BlnI CCTAGG 1 cut(s) 683
BlsI GCNGC 4 cut(s) 35, 172, 175, 201
Bme1390I CCNGG 3 cut(s) 249, 250, 406
BmeT110I CYCGRG 1 cut(s) 248
BmiI GGNNCC 5 cut(s) 223, 254, 277, 283, 828
BmrFI CCNGG 3 cut(s) 249, 250, 406
BmrI ACTGGG 2 cut(s) 67, 1137
BmsI GCATC 3 cut(s) 614, 703, 950
BmuI ACTGGG 2 cut(s) 67, 1137
BpiI GAAGAC 2 cut(s) 85, 363
BplI GAGNNNNNCTC 2 cut(s) 725, 757
BpmI CTGGAG 1 cut(s) 861
BpuMI CCSGG 3 cut(s) 249, 250, 406
BsaHI GRCGYC 3 cut(s) 144, 207, 222
BsaJI CCNNGG 3 cut(s) 217, 248, 683
BsaWI WCCGGW 1 cut(s) 278
BsaXI ACNNNNNCTCC 2 cut(s) 761, 791
Bsc4I CCNNNNNNNGG 4 cut(s) 140, 291, 521, 1131
Bse118I RCCGGY 1 cut(s) 632
Bse1I ACTGG 6 cut(s) 62, 455, 776, 878, 965, 1143
Bse3DI GCAATG 1 cut(s) 1105
BseAI TCCGGA 1 cut(s) 278
BseDI CCNNGG 3 cut(s) 217, 248, 683
BseGI GGATG 3 cut(s) 160, 577, 629
BseLI CCNNNNNNNGG 4 cut(s) 140, 291, 521, 1131
BseMI GCAATG 1 cut(s) 1105
BseMII CTCAG 3 cut(s) 37, 434, 1040
BseNI ACTGG 6 cut(s) 62, 455, 776, 878, 965, 1143
BseXI GCAGC 2 cut(s) 45, 186
BseYI CCCAGC 1 cut(s) 895
BsgI GTGCAG 1 cut(s) 185
Bsh1236I CGCG 2 cut(s) 126, 485
BshFI GGCC 1 cut(s) 498
BshNI GGYRCC 2 cut(s) 221, 252
BsiHKAI GWGCWC 2 cut(s) 247, 419
BsiHKCI CYCGRG 1 cut(s) 248
BsiSI CCGG 5 cut(s) 141, 249, 279, 405, 633
BslFI GGGAC 1 cut(s) 558
BslI CCNNNNNNNGG 4 cut(s) 140, 291, 521, 1131
BsmAI GTCTC 2 cut(s) 845, 1121
BsmFI GGGAC 1 cut(s) 558
BsmI GAATGC 1 cut(s) 1135
BsnI GGCC 1 cut(s) 498
BsoBI CYCGRG 1 cut(s) 248
Bsp1286I GDGCHC 3 cut(s) 247, 419, 516
Bsp13I TCCGGA 1 cut(s) 278
Bsp143I GATC 5 cut(s) 275, 385, 524, 946, 1173
BspACI CCGC 6 cut(s) 5, 31, 168, 171, 174, 950
BspANI GGCC 1 cut(s) 498
BspCNI CTCAG 3 cut(s) 36, 433, 1041
BspEI TCCGGA 1 cut(s) 278
BspFNI CGCG 2 cut(s) 126, 485
BspLI GGNNCC 5 cut(s) 223, 254, 277, 283, 828
BspMI ACCTGC 1 cut(s) 707
BspPI GGATC 5 cut(s) 270, 283, 393, 532, 941
BspT107I GGYRCC 2 cut(s) 221, 252
BsrDI GCAATG 1 cut(s) 1105
BsrFI RCCGGY 1 cut(s) 632
BsrI ACTGG 6 cut(s) 62, 455, 776, 878, 965, 1143
BssAI RCCGGY 1 cut(s) 632
BssECI CCNNGG 3 cut(s) 217, 248, 683
BssMI GATC 5 cut(s) 275, 385, 524, 946, 1173
BssNI GRCGYC 3 cut(s) 144, 207, 222
BssT1I CCWWGG 1 cut(s) 683
Bst4CI ACNGT 5 cut(s) 85, 471, 476, 659, 697
BstACI GRCGYC 3 cut(s) 144, 207, 222
BstC8I GCNNGC 2 cut(s) 496, 974
BstDEI CTNAG 5 cut(s) 23, 232, 420, 834, 1049
BstF5I GGATG 3 cut(s) 160, 577, 629
BstFNI CGCG 2 cut(s) 126, 485
BstH2I RGCGCY 1 cut(s) 225
BstHHI GCGC 3 cut(s) 126, 224, 485
BstKTI GATC 5 cut(s) 278, 388, 527, 949, 1176
BstMAI GTCTC 2 cut(s) 845, 1121
BstMBI GATC 5 cut(s) 275, 385, 524, 946, 1173
BstMWI GCNNNNNNNGC 5 cut(s) 74, 228, 491, 608, 999
BstSCI CCNGG 3 cut(s) 247, 248, 404
BstUI CGCG 2 cut(s) 126, 485
BstV1I GCAGC 2 cut(s) 45, 186
BstV2I GAAGAC 2 cut(s) 85, 363
BstX2I RGATCY 3 cut(s) 275, 946, 1173
BstXI CCANNNNNNTGG 1 cut(s) 382
BstYI RGATCY 3 cut(s) 275, 946, 1173
BsuRI GGCC 1 cut(s) 498
BtsCI GGATG 3 cut(s) 160, 577, 629
BtsIMutI CAGTG 4 cut(s) 655, 769, 885, 1046
BveI ACCTGC 1 cut(s) 707
Cac8I GCNNGC 2 cut(s) 496, 974
CfoI GCGC 3 cut(s) 126, 224, 485
Cfr10I RCCGGY 1 cut(s) 632
Cfr9I CCCGGG 1 cut(s) 248
CseI GACGC 3 cut(s) 133, 215, 474
Csp6I GTAC 4 cut(s) 253, 379, 472, 775
CviAII CATG 6 cut(s) 339, 382, 953, 1095, 1116, 1131
CviQI GTAC 4 cut(s) 253, 379, 472, 775
DdeI CTNAG 5 cut(s) 23, 232, 420, 834, 1049
DinI GGCGCC 1 cut(s) 223
DpnI GATC 5 cut(s) 277, 387, 526, 948, 1175
DpnII GATC 5 cut(s) 275, 385, 524, 946, 1173
DrdI GACNNNNNNGTC 1 cut(s) 143
DseDI GACNNNNNNGTC 1 cut(s) 143
EaeI YGGCCR 1 cut(s) 496
Ecl136II GAGCTC 1 cut(s) 417
Eco130I CCWWGG 1 cut(s) 683
Eco24I GRGCYC 2 cut(s) 419, 516
Eco53kI GAGCTC 1 cut(s) 417
Eco57I CTGAAG 1 cut(s) 957
Eco88I CYCGRG 1 cut(s) 248
EcoICRI GAGCTC 1 cut(s) 417
EcoRI GAATTC 1 cut(s) 1055
EcoT14I CCWWGG 1 cut(s) 683
EcoT38I GRGCYC 2 cut(s) 419, 516
EgeI GGCGCC 1 cut(s) 223
EheI GGCGCC 1 cut(s) 223
ErhI CCWWGG 1 cut(s) 683
FaeI CATG 6 cut(s) 342, 385, 956, 1098, 1119, 1134
FalI AAGNNNNNCTT 4 cut(s) 521, 553, 827, 859
FaqI GGGAC 1 cut(s) 558
FatI CATG 6 cut(s) 338, 381, 952, 1094, 1115, 1130
FblI GTMKAC 1 cut(s) 186
Fnu4HI GCNGC 4 cut(s) 34, 171, 174, 200
FokI GGATG 3 cut(s) 147, 584, 636
FriOI GRGCYC 2 cut(s) 419, 516
Fsp4HI GCNGC 4 cut(s) 34, 171, 174, 200
FspBI CTAG 3 cut(s) 684, 999, 1177
GlaI GCGC 3 cut(s) 125, 223, 484
GluI GCNGC 4 cut(s) 34, 171, 174, 200
GsaI CCCAGC 1 cut(s) 899
GsuI CTGGAG 1 cut(s) 861
HaeII RGCGCY 1 cut(s) 225
HaeIII GGCC 1 cut(s) 498
HapII CCGG 5 cut(s) 141, 249, 279, 405, 633
HgaI GACGC 3 cut(s) 133, 215, 474
HhaI GCGC 3 cut(s) 126, 224, 485
Hin1I GRCGYC 3 cut(s) 144, 207, 222
Hin1II CATG 6 cut(s) 342, 385, 956, 1098, 1119, 1134
Hin6I GCGC 3 cut(s) 124, 222, 483
HinP1I GCGC 3 cut(s) 124, 222, 483
HincII GTYRAC 3 cut(s) 12, 187, 892
HindII GTYRAC 3 cut(s) 12, 187, 892
HindIII AAGCTT 1 cut(s) 530
HinfI GANTC 6 cut(s) 27, 137, 193, 335, 358, 689
HpaII CCGG 5 cut(s) 141, 249, 279, 405, 633
HphI GGTGA 3 cut(s) 73, 139, 1131
Hpy166II GTNNAC 3 cut(s) 12, 187, 892
Hpy188I TCNGA 7 cut(s) 26, 263, 275, 351, 430, 946, 1126
Hpy188III TCNNGA 7 cut(s) 279, 437, 621, 728, 848, 928, 1060
Hpy8I GTNNAC 3 cut(s) 12, 187, 892
Hpy99I CGWCG 3 cut(s) 131, 209, 1032
HpyAV CCTTC 3 cut(s) 88, 107, 166
HpyCH4III ACNGT 5 cut(s) 85, 471, 476, 659, 697
HpyCH4IV ACGT 1 cut(s) 867
HpyCH4V TGCA 9 cut(s) 202, 324, 716, 802, 818, 972, 993, 1018, 1046
HpyF10VI GCNNNNNNNGC 5 cut(s) 74, 228, 491, 608, 999
HpyF3I CTNAG 5 cut(s) 23, 232, 420, 834, 1049
HpySE526I ACGT 1 cut(s) 867
Hsp92I GRCGYC 3 cut(s) 144, 207, 222
Hsp92II CATG 6 cut(s) 342, 385, 956, 1098, 1119, 1134
HspAI GCGC 3 cut(s) 124, 222, 483
KasI GGCGCC 1 cut(s) 221
Kpn2I TCCGGA 1 cut(s) 278
KpnI GGTACC 1 cut(s) 256
Kzo9I GATC 5 cut(s) 275, 385, 524, 946, 1173
LmnI GCTCC 2 cut(s) 250, 281
Lsp1109I GCAGC 2 cut(s) 45, 186
LweI GCATC 3 cut(s) 614, 703, 950
MaeI CTAG 3 cut(s) 684, 999, 1177
MaeII ACGT 1 cut(s) 867
MaeIII GTNAC 1 cut(s) 145
MalI GATC 5 cut(s) 277, 387, 526, 948, 1175
MboI GATC 5 cut(s) 275, 385, 524, 946, 1173
MboII GAAGA 4 cut(s) 85, 363, 758, 863
MfeI CAATTG 1 cut(s) 585
MflI RGATCY 3 cut(s) 275, 946, 1173
MhlI GDGCHC 3 cut(s) 247, 419, 516
MlsI TGGCCA 1 cut(s) 498
MluCI AATT 6 cut(s) 289, 585, 855, 988, 1055, 1090
MluNI TGGCCA 1 cut(s) 498
Mly113I GGCGCC 1 cut(s) 222
MlyI GAGTC 5 cut(s) 21, 131, 187, 344, 352
MmeI TCCRAC 2 cut(s) 138, 188
MnlI CCTC 9 cut(s) 32, 83, 212, 301, 308, 665, 1066, 1070, 1164
Mox20I TGGCCA 1 cut(s) 498
MroI TCCGGA 1 cut(s) 278
MscI TGGCCA 1 cut(s) 498
MseI TTAA 2 cut(s) 447, 861
MslI CAYNNNNRTG 2 cut(s) 660, 878
Msp20I TGGCCA 1 cut(s) 498
MspI CCGG 5 cut(s) 141, 249, 279, 405, 633
MspR9I CCNGG 3 cut(s) 249, 250, 406
MunI CAATTG 1 cut(s) 585
Mva1269I GAATGC 1 cut(s) 1135
MvnI CGCG 2 cut(s) 126, 485
MwoI GCNNNNNNNGC 5 cut(s) 74, 228, 491, 608, 999
NarI GGCGCC 1 cut(s) 222
NciI CCSGG 3 cut(s) 249, 250, 406
NdeII GATC 5 cut(s) 275, 385, 524, 946, 1173
NlaIII CATG 6 cut(s) 342, 385, 956, 1098, 1119, 1134
NlaIV GGNNCC 5 cut(s) 223, 254, 277, 283, 828
NmeAIII GCCGAG 1 cut(s) 242
NmuCI GTSAC 1 cut(s) 145
OliI CACNNNNGTG 1 cut(s) 878
PcsI WCGNNNNNNNCGW 1 cut(s) 81
PctI GAATGC 1 cut(s) 1135
PfeI GAWTC 1 cut(s) 689
PflMI CCANNNNNTGG 1 cut(s) 291
PfoI TCCNGGA 1 cut(s) 404
PkrI GCNGC 4 cut(s) 35, 172, 175, 201
PleI GAGTC 5 cut(s) 21, 131, 187, 343, 352
PluTI GGCGCC 1 cut(s) 225
PpsI GAGTC 5 cut(s) 21, 131, 187, 343, 352
PsiI TTATAA 1 cut(s) 1088
Psp124BI GAGCTC 1 cut(s) 419
PspFI CCCAGC 1 cut(s) 895
PspN4I GGNNCC 5 cut(s) 223, 254, 277, 283, 828
PsuI RGATCY 3 cut(s) 275, 946, 1173
RsaI GTAC 4 cut(s) 254, 380, 473, 776
RsaNI GTAC 4 cut(s) 253, 379, 472, 775
RseI CAYNNNNRTG 2 cut(s) 660, 878
SacI GAGCTC 1 cut(s) 419
SalI GTCGAC 1 cut(s) 185
SaqAI TTAA 2 cut(s) 447, 861
SatI GCNGC 4 cut(s) 34, 171, 174, 200
Sau3AI GATC 5 cut(s) 275, 385, 524, 946, 1173
SchI GAGTC 5 cut(s) 21, 131, 187, 344, 352
ScrFI CCNGG 3 cut(s) 249, 250, 406
SduI GDGCHC 3 cut(s) 247, 419, 516
SfaNI GCATC 3 cut(s) 614, 703, 950
SfoI GGCGCC 1 cut(s) 223
SmaI CCCGGG 1 cut(s) 250
SmiMI CAYNNNNRTG 2 cut(s) 660, 878
Sse9I AATT 6 cut(s) 289, 585, 855, 988, 1055, 1090
SsiI CCGC 6 cut(s) 5, 31, 168, 171, 174, 950
SspDI GGCGCC 1 cut(s) 221
SspMI CTAG 3 cut(s) 684, 999, 1177
SstI GAGCTC 1 cut(s) 419
StyD4I CCNGG 3 cut(s) 247, 248, 404
StyI CCWWGG 1 cut(s) 683
TaaI ACNGT 5 cut(s) 85, 471, 476, 659, 697
TaiI ACGT 1 cut(s) 870
TaqI TCGA 3 cut(s) 186, 670, 729
TasI AATT 6 cut(s) 289, 585, 855, 988, 1055, 1090
TatI WGTACW 1 cut(s) 378
TauI GCSGC 2 cut(s) 173, 176
TfiI GAWTC 1 cut(s) 689
Tru1I TTAA 2 cut(s) 447, 861
Tru9I TTAA 2 cut(s) 447, 861
TscAI CASTG 4 cut(s) 662, 776, 885, 1053
TseFI GTSAC 1 cut(s) 145
TseI GCWGC 2 cut(s) 33, 199
Tsp45I GTSAC 1 cut(s) 145
TspDTI ATGAA 3 cut(s) 588, 1083, 1094
TspGWI ACGGA 2 cut(s) 769, 1016
TspMI CCCGGG 1 cut(s) 248
TspRI CASTG 4 cut(s) 662, 776, 885, 1053
Van91I CCANNNNNTGG 1 cut(s) 291
XapI RAATTY 2 cut(s) 1055, 1090
XmaI CCCGGG 1 cut(s) 248
XmaJI CCTAGG 1 cut(s) 683
XmiI GTMKAC 1 cut(s) 186
XspI CTAG 3 cut(s) 684, 999, 1177
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.