Rh3DG152300

Hydrolyzes acetyl esters in homogalacturonan regions of pectin. In type I primary cell wall, galacturonic acid residues of pectin can be acetylated at the O-2 and O-3 positions. Decreasing the degree of acetylation of pectin gels in vitro alters their physical properties

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3D
OG0001587
Physical Location & Seq
Reverse (-)
13122251 .. 13126990
4740 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3DG152300.1

Sequence Viewer

Length: 789 bp
ATGGCGGGGTCAACGGCGAACCTCAGACTCCGCAGCCTTGTATGGTGGAGAAAGGACTGGGCAATCGCCACCCTCGCCTTCACCGTCATCGTCTTCCTTCTCACTCTCATCTCCAACTCTTTGCGCGACGAACCAGACTCCGGCGTCACCAACCCCTTCCATCCAACCGCCGCCGCCGATTTGGTCGACTTGACTCTGCTGCACGACGCCGAACGCCGAGGCGCCCTTTGCTTAGATGGGAGTGCTCCCGGGTACCATTTCCGAAAGGGGTTCGGATCCGGAGCCAAAAATTGGTTGCTTCACATTGAGGGTGGAGGTTGGTGCAATACAATAGAGTCATGTTCTTTTCGGAAAAGGACTCCATTAGGGTCTTCCAAGTACATGGATCGTAGGGTTCAGTTTTCCGGGATTTTGAGCTCTCAGCCGTCAGAAAATCCTGACTTCTTTAACTGGAACAAAGTCAAAATACGGTACTGTGATGGCGCGTCTTTTGCTGGCCACCCCGAAAATGAGCCCAAGAATGGATCAAAGCTTTACTTTAGGGGACAGCTCATCTGGGAAGCACTTATGGATGAACTCTTGTCAATTGGCTTGTCAAAGGCGAAACAGGCACTTCTTTCTGGATGCTCTGCCGGTGGGTTGGCAACTCTTATACACTGTGATGACTTTCGAGGTCTTTTACCTAGGGATTCAACTGTCAAATGTCTTGCTGATGCAGGATCACCATCAAGAAAGGAAGAGATGAACAACTCTCCTTCGGAGACGTCTTCTTCATTGGTGGAGTATTGA

Protein Analysis

262

Amino Acids

28.78

Weight (kDa)

7.61

Isoelectric Point (pI)

45.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PAE PF03283 59 - 241 1.1e-88 Pectinacetylesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 143
AatII GACGTC 1 cut(s) 767
Acc65I GGTACC 1 cut(s) 252
AccB1I GGYRCC 2 cut(s) 221, 252
AccB7I CCANNNNNTGG 1 cut(s) 291
AccI GTMKAC 1 cut(s) 186
AccII CGCG 2 cut(s) 126, 485
AccIII TCCGGA 1 cut(s) 278
AciI CCGC 5 cut(s) 5, 31, 168, 171, 174
AclWI GGATC 5 cut(s) 270, 283, 393, 532, 727
AcoI YGGCCR 1 cut(s) 496
AcyI GRCGYC 4 cut(s) 144, 207, 222, 764
AfaI GTAC 3 cut(s) 254, 380, 473
AfiI CCNNNNNNNGG 3 cut(s) 140, 291, 521
AgsI TTSAA 1 cut(s) 693
AluBI AGCT 3 cut(s) 417, 532, 550
AluI AGCT 3 cut(s) 417, 532, 550
Alw21I GWGCWC 2 cut(s) 247, 419
Alw26I GTCTC 1 cut(s) 755
AlwI GGATC 5 cut(s) 270, 283, 393, 532, 727
Ama87I CYCGRG 1 cut(s) 248
Aor13HI TCCGGA 1 cut(s) 278
AoxI GGCC 1 cut(s) 496
ApeKI GCWGC 2 cut(s) 33, 199
Asp718I GGTACC 1 cut(s) 252
AspA2I CCTAGG 1 cut(s) 683
AspLEI GCGC 3 cut(s) 126, 224, 485
AsuC2I CCSGG 3 cut(s) 249, 250, 406
AsuHPI GGTGA 3 cut(s) 73, 139, 714
AvaI CYCGRG 1 cut(s) 248
AvrII CCTAGG 1 cut(s) 683
BalI TGGCCA 1 cut(s) 498
BamHI GGATCC 1 cut(s) 275
BanI GGYRCC 2 cut(s) 221, 252
BanII GRGCYC 2 cut(s) 419, 516
BbsI GAAGAC 3 cut(s) 85, 363, 759
Bbv12I GWGCWC 2 cut(s) 247, 419
BbvI GCAGC 2 cut(s) 45, 186
BccI CCATC 4 cut(s) 168, 230, 473, 733
BceAI ACGGC 2 cut(s) 30, 409
BcnI CCSGG 3 cut(s) 249, 250, 406
BcoDI GTCTC 1 cut(s) 755
BfaI CTAG 1 cut(s) 684
BfoI RGCGCY 1 cut(s) 225
BisI GCNGC 4 cut(s) 34, 171, 174, 200
BlnI CCTAGG 1 cut(s) 683
BlsI GCNGC 4 cut(s) 35, 172, 175, 201
Bme1390I CCNGG 3 cut(s) 249, 250, 406
BmeT110I CYCGRG 1 cut(s) 248
BmiI GGNNCC 4 cut(s) 223, 254, 277, 283
BmrFI CCNGG 3 cut(s) 249, 250, 406
BmrI ACTGGG 1 cut(s) 67
BmsI GCATC 2 cut(s) 614, 703
BmuI ACTGGG 1 cut(s) 67
BpiI GAAGAC 3 cut(s) 85, 363, 759
BpuMI CCSGG 3 cut(s) 249, 250, 406
BsaBI GATNNNNATC 1 cut(s) 724
BsaHI GRCGYC 4 cut(s) 144, 207, 222, 764
BsaJI CCNNGG 3 cut(s) 217, 248, 683
BsaWI WCCGGW 1 cut(s) 278
Bsc4I CCNNNNNNNGG 3 cut(s) 140, 291, 521
Bse118I RCCGGY 1 cut(s) 632
Bse1I ACTGG 2 cut(s) 62, 455
Bse8I GATNNNNATC 1 cut(s) 724
BseAI TCCGGA 1 cut(s) 278
BseDI CCNNGG 3 cut(s) 217, 248, 683
BseGI GGATG 3 cut(s) 160, 577, 629
BseJI GATNNNNATC 1 cut(s) 724
BseLI CCNNNNNNNGG 3 cut(s) 140, 291, 521
BseMII CTCAG 2 cut(s) 37, 434
BseNI ACTGG 2 cut(s) 62, 455
BseXI GCAGC 2 cut(s) 45, 186
BsgI GTGCAG 1 cut(s) 185
Bsh1236I CGCG 2 cut(s) 126, 485
BshFI GGCC 1 cut(s) 498
BshNI GGYRCC 2 cut(s) 221, 252
BsiHKAI GWGCWC 2 cut(s) 247, 419
BsiHKCI CYCGRG 1 cut(s) 248
BsiSI CCGG 5 cut(s) 141, 249, 279, 405, 633
BslFI GGGAC 1 cut(s) 558
BslI CCNNNNNNNGG 3 cut(s) 140, 291, 521
BsmAI GTCTC 1 cut(s) 755
BsmBI CGTCTC 1 cut(s) 755
BsmFI GGGAC 1 cut(s) 558
BsnI GGCC 1 cut(s) 498
BsoBI CYCGRG 1 cut(s) 248
Bsp1286I GDGCHC 3 cut(s) 247, 419, 516
Bsp13I TCCGGA 1 cut(s) 278
Bsp143I GATC 4 cut(s) 275, 385, 524, 719
BspACI CCGC 5 cut(s) 5, 31, 168, 171, 174
BspANI GGCC 1 cut(s) 498
BspCNI CTCAG 2 cut(s) 36, 433
BspEI TCCGGA 1 cut(s) 278
BspFNI CGCG 2 cut(s) 126, 485
BspLI GGNNCC 4 cut(s) 223, 254, 277, 283
BspPI GGATC 5 cut(s) 270, 283, 393, 532, 727
BspT107I GGYRCC 2 cut(s) 221, 252
BsrFI RCCGGY 1 cut(s) 632
BsrI ACTGG 2 cut(s) 62, 455
BssAI RCCGGY 1 cut(s) 632
BssECI CCNNGG 3 cut(s) 217, 248, 683
BssMI GATC 4 cut(s) 275, 385, 524, 719
BssNI GRCGYC 4 cut(s) 144, 207, 222, 764
BssT1I CCWWGG 1 cut(s) 683
Bst4CI ACNGT 5 cut(s) 85, 471, 476, 659, 697
Bst6I CTCTTC 1 cut(s) 732
BstACI GRCGYC 4 cut(s) 144, 207, 222, 764
BstC8I GCNNGC 1 cut(s) 496
BstDEI CTNAG 3 cut(s) 23, 232, 420
BstF5I GGATG 3 cut(s) 160, 577, 629
BstFNI CGCG 2 cut(s) 126, 485
BstH2I RGCGCY 1 cut(s) 225
BstHHI GCGC 3 cut(s) 126, 224, 485
BstKTI GATC 4 cut(s) 278, 388, 527, 722
BstMAI GTCTC 1 cut(s) 755
BstMBI GATC 4 cut(s) 275, 385, 524, 719
BstMWI GCNNNNNNNGC 4 cut(s) 74, 228, 491, 608
BstSCI CCNGG 3 cut(s) 247, 248, 404
BstUI CGCG 2 cut(s) 126, 485
BstV1I GCAGC 2 cut(s) 45, 186
BstV2I GAAGAC 3 cut(s) 85, 363, 759
BstX2I RGATCY 1 cut(s) 275
BstXI CCANNNNNNTGG 1 cut(s) 382
BstYI RGATCY 1 cut(s) 275
BsuRI GGCC 1 cut(s) 498
BtsCI GGATG 3 cut(s) 160, 577, 629
BtsIMutI CAGTG 1 cut(s) 655
Cac8I GCNNGC 1 cut(s) 496
CfoI GCGC 3 cut(s) 126, 224, 485
Cfr10I RCCGGY 1 cut(s) 632
Cfr9I CCCGGG 1 cut(s) 248
CseI GACGC 3 cut(s) 133, 215, 474
Csp6I GTAC 3 cut(s) 253, 379, 472
CviAII CATG 2 cut(s) 339, 382
CviJI RGCY 9 cut(s) 36, 284, 417, 424, 498, 514, 532, 550, 591
CviKI_1 RGCY 9 cut(s) 36, 284, 417, 424, 498, 514, 532, 550, 591
CviQI GTAC 3 cut(s) 253, 379, 472
DdeI CTNAG 3 cut(s) 23, 232, 420
DinI GGCGCC 1 cut(s) 223
DpnI GATC 4 cut(s) 277, 387, 526, 721
DpnII GATC 4 cut(s) 275, 385, 524, 719
DrdI GACNNNNNNGTC 1 cut(s) 143
DseDI GACNNNNNNGTC 1 cut(s) 143
EaeI YGGCCR 1 cut(s) 496
Eam1104I CTCTTC 1 cut(s) 732
EarI CTCTTC 1 cut(s) 732
Ecl136II GAGCTC 1 cut(s) 417
Eco130I CCWWGG 1 cut(s) 683
Eco24I GRGCYC 2 cut(s) 419, 516
Eco53kI GAGCTC 1 cut(s) 417
Eco88I CYCGRG 1 cut(s) 248
EcoICRI GAGCTC 1 cut(s) 417
EcoT14I CCWWGG 1 cut(s) 683
EcoT38I GRGCYC 2 cut(s) 419, 516
EgeI GGCGCC 1 cut(s) 223
EheI GGCGCC 1 cut(s) 223
ErhI CCWWGG 1 cut(s) 683
Esp3I CGTCTC 1 cut(s) 755
FaeI CATG 2 cut(s) 342, 385
FaiI YATR 5 cut(s) 43, 340, 383, 569, 653
FalI AAGNNNNNCTT 2 cut(s) 521, 553
FaqI GGGAC 1 cut(s) 558
FatI CATG 2 cut(s) 338, 381
FblI GTMKAC 1 cut(s) 186
Fnu4HI GCNGC 4 cut(s) 34, 171, 174, 200
FokI GGATG 3 cut(s) 147, 584, 636
FriOI GRGCYC 2 cut(s) 419, 516
Fsp4HI GCNGC 4 cut(s) 34, 171, 174, 200
FspBI CTAG 1 cut(s) 684
GlaI GCGC 3 cut(s) 125, 223, 484
GluI GCNGC 4 cut(s) 34, 171, 174, 200
HaeII RGCGCY 1 cut(s) 225
HaeIII GGCC 1 cut(s) 498
HapII CCGG 5 cut(s) 141, 249, 279, 405, 633
HgaI GACGC 3 cut(s) 133, 215, 474
HhaI GCGC 3 cut(s) 126, 224, 485
Hin1I GRCGYC 4 cut(s) 144, 207, 222, 764
Hin1II CATG 2 cut(s) 342, 385
Hin6I GCGC 3 cut(s) 124, 222, 483
HinP1I GCGC 3 cut(s) 124, 222, 483
HincII GTYRAC 2 cut(s) 12, 187
HindII GTYRAC 2 cut(s) 12, 187
HindIII AAGCTT 1 cut(s) 530
HinfI GANTC 6 cut(s) 27, 137, 193, 335, 358, 689
HpaII CCGG 5 cut(s) 141, 249, 279, 405, 633
HphI GGTGA 3 cut(s) 73, 139, 714
Hpy166II GTNNAC 2 cut(s) 12, 187
Hpy188I TCNGA 6 cut(s) 26, 263, 275, 351, 430, 760
Hpy188III TCNNGA 4 cut(s) 279, 437, 621, 729
Hpy8I GTNNAC 2 cut(s) 12, 187
Hpy99I CGWCG 2 cut(s) 131, 209
HpyAV CCTTC 4 cut(s) 88, 107, 166, 765
HpyCH4III ACNGT 5 cut(s) 85, 471, 476, 659, 697
HpyCH4IV ACGT 1 cut(s) 764
HpyCH4V TGCA 3 cut(s) 202, 324, 716
HpyF10VI GCNNNNNNNGC 4 cut(s) 74, 228, 491, 608
HpyF3I CTNAG 3 cut(s) 23, 232, 420
HpySE526I ACGT 1 cut(s) 764
Hsp92I GRCGYC 4 cut(s) 144, 207, 222, 764
Hsp92II CATG 2 cut(s) 342, 385
HspAI GCGC 3 cut(s) 124, 222, 483
KasI GGCGCC 1 cut(s) 221
Kpn2I TCCGGA 1 cut(s) 278
KpnI GGTACC 1 cut(s) 256
Kzo9I GATC 4 cut(s) 275, 385, 524, 719
LmnI GCTCC 2 cut(s) 250, 281
Lsp1109I GCAGC 2 cut(s) 45, 186
LweI GCATC 2 cut(s) 614, 703
MaeI CTAG 1 cut(s) 684
MaeII ACGT 1 cut(s) 764
MaeIII GTNAC 1 cut(s) 145
MalI GATC 4 cut(s) 277, 387, 526, 721
MboI GATC 4 cut(s) 275, 385, 524, 719
MboII GAAGA 5 cut(s) 85, 363, 749, 759, 762
MfeI CAATTG 1 cut(s) 585
MflI RGATCY 1 cut(s) 275
MhlI GDGCHC 3 cut(s) 247, 419, 516
MlsI TGGCCA 1 cut(s) 498
MluCI AATT 2 cut(s) 289, 585
MluNI TGGCCA 1 cut(s) 498
Mly113I GGCGCC 1 cut(s) 222
MlyI GAGTC 5 cut(s) 21, 131, 187, 344, 352
MmeI TCCRAC 2 cut(s) 138, 188
MnlI CCTC 6 cut(s) 32, 83, 212, 301, 308, 665
Mox20I TGGCCA 1 cut(s) 498
MroI TCCGGA 1 cut(s) 278
MscI TGGCCA 1 cut(s) 498
MseI TTAA 1 cut(s) 447
MslI CAYNNNNRTG 1 cut(s) 660
Msp20I TGGCCA 1 cut(s) 498
MspI CCGG 5 cut(s) 141, 249, 279, 405, 633
MspR9I CCNGG 3 cut(s) 249, 250, 406
MunI CAATTG 1 cut(s) 585
MvnI CGCG 2 cut(s) 126, 485
MwoI GCNNNNNNNGC 4 cut(s) 74, 228, 491, 608
NarI GGCGCC 1 cut(s) 222
NciI CCSGG 3 cut(s) 249, 250, 406
NdeII GATC 4 cut(s) 275, 385, 524, 719
NlaIII CATG 2 cut(s) 342, 385
NlaIV GGNNCC 4 cut(s) 223, 254, 277, 283
NmeAIII GCCGAG 1 cut(s) 242
NmuCI GTSAC 1 cut(s) 145
PcsI WCGNNNNNNNCGW 1 cut(s) 81
PfeI GAWTC 1 cut(s) 689
PflMI CCANNNNNTGG 1 cut(s) 291
PfoI TCCNGGA 1 cut(s) 404
PkrI GCNGC 4 cut(s) 35, 172, 175, 201
PleI GAGTC 5 cut(s) 21, 131, 187, 343, 352
PluTI GGCGCC 1 cut(s) 225
PpsI GAGTC 5 cut(s) 21, 131, 187, 343, 352
Psp124BI GAGCTC 1 cut(s) 419
PspN4I GGNNCC 4 cut(s) 223, 254, 277, 283
PsuI RGATCY 1 cut(s) 275
RsaI GTAC 3 cut(s) 254, 380, 473
RsaNI GTAC 3 cut(s) 253, 379, 472
RseI CAYNNNNRTG 1 cut(s) 660
SacI GAGCTC 1 cut(s) 419
SalI GTCGAC 1 cut(s) 185
SaqAI TTAA 1 cut(s) 447
SatI GCNGC 4 cut(s) 34, 171, 174, 200
Sau3AI GATC 4 cut(s) 275, 385, 524, 719
SchI GAGTC 5 cut(s) 21, 131, 187, 344, 352
ScrFI CCNGG 3 cut(s) 249, 250, 406
SduI GDGCHC 3 cut(s) 247, 419, 516
SetI ASST 8 cut(s) 24, 319, 419, 534, 552, 676, 685, 767
SfaNI GCATC 2 cut(s) 614, 703
SfoI GGCGCC 1 cut(s) 223
SmaI CCCGGG 1 cut(s) 250
SmiMI CAYNNNNRTG 1 cut(s) 660
Sse9I AATT 2 cut(s) 289, 585
SsiI CCGC 5 cut(s) 5, 31, 168, 171, 174
SspDI GGCGCC 1 cut(s) 221
SspMI CTAG 1 cut(s) 684
SstI GAGCTC 1 cut(s) 419
StyD4I CCNGG 3 cut(s) 247, 248, 404
StyI CCWWGG 1 cut(s) 683
TaaI ACNGT 5 cut(s) 85, 471, 476, 659, 697
TaiI ACGT 1 cut(s) 767
TaqI TCGA 2 cut(s) 186, 670
TasI AATT 2 cut(s) 289, 585
TatI WGTACW 1 cut(s) 378
TauI GCSGC 2 cut(s) 173, 176
TfiI GAWTC 1 cut(s) 689
Tru1I TTAA 1 cut(s) 447
Tru9I TTAA 1 cut(s) 447
TscAI CASTG 1 cut(s) 662
TseFI GTSAC 1 cut(s) 145
TseI GCWGC 2 cut(s) 33, 199
Tsp45I GTSAC 1 cut(s) 145
TspDTI ATGAA 3 cut(s) 588, 758, 762
TspMI CCCGGG 1 cut(s) 248
TspRI CASTG 1 cut(s) 662
Van91I CCANNNNNTGG 1 cut(s) 291
XmaI CCCGGG 1 cut(s) 248
XmaJI CCTAGG 1 cut(s) 683
XmiI GTMKAC 1 cut(s) 186
XspI CTAG 1 cut(s) 684
ZraI GACGTC 1 cut(s) 765
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.