Rh6AG044200

Hydrolyzes acetyl esters in homogalacturonan regions of pectin. In type I primary cell wall, galacturonic acid residues of pectin can be acetylated at the O-2 and O-3 positions. Decreasing the degree of acetylation of pectin gels in vitro alters their physical properties

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6A
OG0001587
Physical Location & Seq
Forward (+)
6460243 .. 6465970
5728 bp
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UTR
Exon/CDS
Intron
Rh6AG044200.1

Sequence Viewer

Length: 579 bp
ATGGATGAGTTGATGTTCAAGGGAATGCAGAATGCTAACAAGGCTCTTCTTTCTGGATGTTCCACTGGGGTTCTGGCATCCATGCTACACTGTGATGTGTTCTGGGACTTGTTTCCATCCAGTACCACAGTGAAATGTTCCTTGATGCTCAGCCTTTTCTCTTTCTGCAGTCAGCATGCCTTCCACTACGACTGCTACCAAAACGGCTATGCGGCTGTGAACCACTTTAATCTGGTGTGTCCATTGTGTTGCCAAAAGATTTGCGATGGCAACATCCACATATACATGTTACTTGACACGGGTCTATCCGAGGACCTTCAGGCTTTTCAGCTTCCGACCCACCCTCCCACTCCACCCTCTCTACCACAAAACTTCATTCCACTGCCGGAGGATCCCATCATTCCACCAGAGTTCCCGGGCTTTGAACCAGACGATGAACCCAACCCCATCCTTGAGTTCACCGGCTTTGAAATAGGGGACGAAGCCAACCCCAACCCTAATCCCAATCTGGTTCAAGAACCCAACCCGAACCCGCCTCAACAATCAATACCCGCCCAACAAGCCAACTCAGCCCAATAA

Protein Analysis

192

Amino Acids

21.19

Weight (kDa)

4.2

Isoelectric Point (pI)

49.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PAE PF03283 1 - 48 2.3e-14 Pectinacetylesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 3 cut(s) 212, 533, 552
AclWI GGATC 2 cut(s) 386, 399
AcuI CTGAAG 1 cut(s) 302
AfaI GTAC 1 cut(s) 124
AflIII ACRYGT 1 cut(s) 285
AgsI TTSAA 4 cut(s) 19, 425, 470, 515
AluBI AGCT 1 cut(s) 331
AluI AGCT 1 cut(s) 331
AlwI GGATC 2 cut(s) 386, 399
Ama87I CYCGRG 1 cut(s) 415
AspS9I GGNCC 1 cut(s) 313
AsuC2I CCSGG 2 cut(s) 416, 417
AsuHPI GGTGA 1 cut(s) 451
AvaI CYCGRG 1 cut(s) 415
AvaII GGWCC 1 cut(s) 313
BamHI GGATCC 1 cut(s) 391
BccI CCATC 4 cut(s) 124, 260, 404, 455
BceAI ACGGC 1 cut(s) 220
BcnI CCSGG 2 cut(s) 416, 417
BfmI CTRYAG 1 cut(s) 166
BisI GCNGC 1 cut(s) 213
BlpI GCTNAGC 1 cut(s) 149
BlsI GCNGC 1 cut(s) 214
Bme1390I CCNGG 2 cut(s) 416, 417
Bme18I GGWCC 1 cut(s) 313
BmeT110I CYCGRG 1 cut(s) 415
BmgT120I GGNCC 1 cut(s) 313
BmiI GGNNCC 1 cut(s) 393
BmrFI CCNGG 2 cut(s) 416, 417
BmrI ACTGGG 1 cut(s) 75
BmsI GCATC 2 cut(s) 86, 135
BmuI ACTGGG 1 cut(s) 75
BoxI GACNNNNGTC 1 cut(s) 300
Bpu1102I GCTNAGC 1 cut(s) 149
BpuEI CTTGAG 1 cut(s) 473
BpuMI CCSGG 2 cut(s) 416, 417
BsaJI CCNNGG 2 cut(s) 309, 415
BsaXI ACNNNNNCTCC 2 cut(s) 328, 358
Bse118I RCCGGY 1 cut(s) 461
Bse1I ACTGG 2 cut(s) 70, 120
BseDI CCNNGG 2 cut(s) 309, 415
BseGI GGATG 6 cut(s) 10, 62, 77, 116, 273, 447
BseMII CTCAG 1 cut(s) 163
BseNI ACTGG 2 cut(s) 70, 120
BsiHKCI CYCGRG 1 cut(s) 415
BsiSI CCGG 3 cut(s) 386, 416, 462
BslFI GGGAC 2 cut(s) 119, 491
BsmFI GGGAC 2 cut(s) 119, 491
BsmI GAATGC 2 cut(s) 30, 37
BsoBI CYCGRG 1 cut(s) 415
Bsp143I GATC 1 cut(s) 391
Bsp1720I GCTNAGC 1 cut(s) 149
BspACI CCGC 3 cut(s) 212, 533, 552
BspCNI CTCAG 1 cut(s) 162
BspLI GGNNCC 1 cut(s) 393
BspMAI CTGCAG 1 cut(s) 170
BspPI GGATC 2 cut(s) 386, 399
BspQI GCTCTTC 1 cut(s) 51
BsrFI RCCGGY 1 cut(s) 461
BsrI ACTGG 2 cut(s) 70, 120
BssAI RCCGGY 1 cut(s) 461
BssECI CCNNGG 2 cut(s) 309, 415
BssMI GATC 1 cut(s) 391
Bst4CI ACNGT 2 cut(s) 92, 130
Bst6I CTCTTC 1 cut(s) 51
BstC8I GCNNGC 1 cut(s) 177
BstDEI CTNAG 2 cut(s) 149, 568
BstF5I GGATG 6 cut(s) 10, 62, 77, 116, 273, 447
BstKTI GATC 1 cut(s) 394
BstMBI GATC 1 cut(s) 391
BstMWI GCNNNNNNNGC 3 cut(s) 41, 560, 569
BstNSI RCATGY 2 cut(s) 179, 289
BstPAI GACNNNNGTC 1 cut(s) 300
BstSCI CCNGG 2 cut(s) 414, 415
BstSFI CTRYAG 1 cut(s) 166
BstX2I RGATCY 1 cut(s) 391
BstYI RGATCY 1 cut(s) 391
BtgZI GCGATG 1 cut(s) 279
BtsCI GGATG 6 cut(s) 10, 62, 77, 116, 273, 447
BtsI GCAGTG 1 cut(s) 380
BtsIMutI CAGTG 4 cut(s) 63, 88, 135, 380
Cac8I GCNNGC 1 cut(s) 177
Cfr10I RCCGGY 1 cut(s) 461
Cfr13I GGNCC 1 cut(s) 313
Cfr9I CCCGGG 1 cut(s) 415
Csp6I GTAC 1 cut(s) 123
CviAII CATG 3 cut(s) 82, 176, 286
CviQI GTAC 1 cut(s) 123
DdeI CTNAG 2 cut(s) 149, 568
DpnI GATC 1 cut(s) 393
DpnII GATC 1 cut(s) 391
Eam1104I CTCTTC 1 cut(s) 51
EarI CTCTTC 1 cut(s) 51
Eco47I GGWCC 1 cut(s) 313
Eco57I CTGAAG 1 cut(s) 302
Eco88I CYCGRG 1 cut(s) 415
EcoO109I RGGNCCY 1 cut(s) 313
FaeI CATG 3 cut(s) 85, 179, 289
FaiI YATR 6 cut(s) 83, 177, 210, 281, 283, 287
FaqI GGGAC 2 cut(s) 119, 491
FatI CATG 3 cut(s) 81, 175, 285
FauI CCCGC 2 cut(s) 540, 559
Fnu4HI GCNGC 1 cut(s) 213
FokI GGATG 6 cut(s) 17, 64, 69, 103, 260, 434
Fsp4HI GCNGC 1 cut(s) 213
GluI GCNGC 1 cut(s) 213
HapII CCGG 3 cut(s) 386, 416, 462
Hin1II CATG 3 cut(s) 85, 179, 289
HpaII CCGG 3 cut(s) 386, 416, 462
HphI GGTGA 1 cut(s) 451
Hpy166II GTNNAC 2 cut(s) 220, 459
Hpy188I TCNGA 2 cut(s) 310, 336
Hpy188III TCNNGA 2 cut(s) 54, 515
Hpy8I GTNNAC 2 cut(s) 220, 459
HpyAV CCTTC 2 cut(s) 190, 326
HpyCH4III ACNGT 2 cut(s) 92, 130
HpyCH4V TGCA 2 cut(s) 28, 168
HpyF10VI GCNNNNNNNGC 3 cut(s) 41, 560, 569
HpyF3I CTNAG 2 cut(s) 149, 568
Hsp92II CATG 3 cut(s) 85, 179, 289
Kzo9I GATC 1 cut(s) 391
LguI GCTCTTC 1 cut(s) 51
LweI GCATC 2 cut(s) 86, 135
MaeIII GTNAC 1 cut(s) 288
MalI GATC 1 cut(s) 393
MboI GATC 1 cut(s) 391
MboII GAAGA 1 cut(s) 38
MflI RGATCY 1 cut(s) 391
MmeI TCCRAC 1 cut(s) 359
MnlI CCTC 5 cut(s) 304, 354, 367, 382, 546
MseI TTAA 1 cut(s) 228
MslI CAYNNNNRTG 2 cut(s) 93, 284
MspI CCGG 3 cut(s) 386, 416, 462
MspR9I CCNGG 2 cut(s) 416, 417
Mva1269I GAATGC 2 cut(s) 30, 37
MwoI GCNNNNNNNGC 3 cut(s) 41, 560, 569
NciI CCSGG 2 cut(s) 416, 417
NdeII GATC 1 cut(s) 391
NlaIII CATG 3 cut(s) 85, 179, 289
NlaIV GGNNCC 1 cut(s) 393
NspI RCATGY 2 cut(s) 179, 289
PaeI GCATGC 1 cut(s) 179
PciI ACATGT 1 cut(s) 285
PciSI GCTCTTC 1 cut(s) 51
PctI GAATGC 2 cut(s) 30, 37
PkrI GCNGC 1 cut(s) 214
PpuMI RGGWCCY 1 cut(s) 313
PscI ACATGT 1 cut(s) 285
PshAI GACNNNNGTC 1 cut(s) 300
Psp5II RGGWCCY 1 cut(s) 313
PspN4I GGNNCC 1 cut(s) 393
PspPI GGNCC 1 cut(s) 313
PspPPI RGGWCCY 1 cut(s) 313
PstI CTGCAG 1 cut(s) 170
PsuI RGATCY 1 cut(s) 391
RsaI GTAC 1 cut(s) 124
RsaNI GTAC 1 cut(s) 123
RseI CAYNNNNRTG 2 cut(s) 93, 284
SapI GCTCTTC 1 cut(s) 51
SaqAI TTAA 1 cut(s) 228
SatI GCNGC 1 cut(s) 213
Sau3AI GATC 1 cut(s) 391
Sau96I GGNCC 1 cut(s) 313
ScrFI CCNGG 2 cut(s) 416, 417
SetI ASST 2 cut(s) 318, 333
SfaNI GCATC 2 cut(s) 86, 135
SfcI CTRYAG 1 cut(s) 166
SinI GGWCC 1 cut(s) 313
SmaI CCCGGG 1 cut(s) 417
SmiMI CAYNNNNRTG 2 cut(s) 93, 284
SmlI CTYRAG 1 cut(s) 452
SmoI CTYRAG 1 cut(s) 452
SphI GCATGC 1 cut(s) 179
SsiI CCGC 3 cut(s) 212, 533, 552
StyD4I CCNGG 2 cut(s) 414, 415
TaaI ACNGT 2 cut(s) 92, 130
TauI GCSGC 1 cut(s) 215
Tru1I TTAA 1 cut(s) 228
Tru9I TTAA 1 cut(s) 228
TscAI CASTG 4 cut(s) 70, 95, 135, 387
TspDTI ATGAA 2 cut(s) 364, 450
TspMI CCCGGG 1 cut(s) 415
TspRI CASTG 4 cut(s) 70, 95, 135, 387
VpaK11BI GGWCC 1 cut(s) 313
XceI RCATGY 2 cut(s) 179, 289
XcmI CCANNNNNNNNNTGG 1 cut(s) 70
XmaI CCCGGG 1 cut(s) 415
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.