Rw3G012320

Hydrolyzes acetyl esters in homogalacturonan regions of pectin. In type I primary cell wall, galacturonic acid residues of pectin can be acetylated at the O-2 and O-3 positions. Decreasing the degree of acetylation of pectin gels in vitro alters their physical properties

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr3
OG0001587
Physical Location & Seq
Reverse (-)
12150392 .. 12153889
3498 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw3G012320.1

Sequence Viewer

Length: 1314 bp
ATGGCGGGGTCAACGGCGAACCTCGGACTCCGCAGCCTTGTATGGTGGAGAAAGTGGGCCAGAAAGGACTGGGCAATCGCCACCCTCGCCTTCACCGTCATCGTCTTCCTTCTCACTCTCATCTCCAACTCTTTGCCCGACGAGCCAGACTCCGGCGTCACCAACCCCTTCCATCCAACCACCGCCGCCGATTTGGTCGACTTGACTCTGCTGCACGACGCCGAACGCAGAGGCGCCCTTTGCTTAGATGGGAGTGCTCCCGGGTACCATTTCCGAAAGGGGTTCGGATCCGGAGCCAAAAATTGGCTGCTTCACATTGAGGGTGGAGGTTGGTGCAATACAATAGAGTCATGTTCTTTTCGGAAAAGGACTCCATTAGGGTCTTCCAAGTACATGGATCGTAGGGTTCAGTTTTCCGGGATTTTGAGCTCTCAGCCGTCAGAAAATCCTGACTTCTTTAACTGGAACAAAGTCAAAATACGGTACTGTGATGGCGCGTCTTTTGCTGGCCACCCCGAAAATGAGCCCAAGAATGGATCAAAGCTTTACTTTAGGGGACAGCTCATCTGGGAAGCACTTATGGATGAACTCTTGTCAATTGGCTTGTCAAAGGCAAAACAGGCACTTCTTTCTGGATGCTCTGCTGGTGGGTTGGCAACTCTTATACACTGTGATGACTTTCGAGGTCTTTTACCTAGGGATTCAACTGTCAAATGTCTTGCTGATGCAGGTTTTTTTCTCGATGAGAAAGATGTTAACCAAAACTGGACCATGCGAACTTTTTATCGTGATGTTGTGGTTCTTCAGGATTTAGCAAAAAGTTTGCACAAGGATTGTGTTGCAAGAAAGGAACCAGCTAAGTGTCTCTTTCCCGAAGAGATTATTAAACACGTTAGCACTCCAGTGTTCCTTGTCAACCCAGCTTATGATTTTTGGCAGATACAACATATCTTGATACCTGAAGCATCAGATCCGCATGGCTACTGGAAAAAGTGCAAGCTGAACATCTATAATTGCAATCCTAGCCAGATTGAAATACTGCAAGGTTTCCGTCGTTCTATGCTAAATGCATTGAGCGAATTCCAGAAAAATAAGGAGGGAGGAACTTTTATAAATTCATGTTTTATTCATTGCCAGACATGGTTTTCCGAGACATGGCATTCACCCAGTTCTCCACGAATAAACAATAAGACAATTGCAGAGTCTGTGGGTGATTGGTACTTTAATCGAAATGTTGCAAAGCAAATTGACTGCCCATTTCCATGCAATCCCACCTGTTATCATATGAATTTCACTCAATTTTCGCGAGGTTGA

Protein Analysis

437

Amino Acids

49.4

Weight (kDa)

8.4

Isoelectric Point (pI)

45.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PAE PF03283 63 - 426 1.7e-165 Pectinacetylesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1112
AasI GACNNNNNNGTC 1 cut(s) 155
Acc36I ACCTGC 1 cut(s) 719
Acc65I GGTACC 1 cut(s) 264
AccB1I GGYRCC 2 cut(s) 233, 264
AccB7I CCANNNNNTGG 1 cut(s) 303
AccI GTMKAC 1 cut(s) 198
AccII CGCG 2 cut(s) 497, 1306
AccIII TCCGGA 1 cut(s) 290
AciI CCGC 5 cut(s) 5, 31, 183, 186, 974
AclWI GGATC 5 cut(s) 282, 295, 405, 544, 965
AcoI YGGCCR 1 cut(s) 508
AcsI RAATTY 3 cut(s) 1079, 1114, 1288
AcuI CTGAAG 2 cut(s) 788, 981
AcyI GRCGYC 3 cut(s) 156, 219, 234
AfaI GTAC 4 cut(s) 266, 392, 485, 1220
AfiI CCNNNNNNNGG 4 cut(s) 152, 303, 533, 1155
AflIII ACRYGT 1 cut(s) 889
AgsI TTSAA 2 cut(s) 705, 1034
AleI CACNNNNGTG 1 cut(s) 902
AluBI AGCT 6 cut(s) 429, 544, 562, 857, 923, 1000
AluI AGCT 6 cut(s) 429, 544, 562, 857, 923, 1000
Alw21I GWGCWC 2 cut(s) 259, 431
Alw26I GTCTC 2 cut(s) 869, 1145
AlwI GGATC 5 cut(s) 282, 295, 405, 544, 965
AlwNI CAGNNNCTG 1 cut(s) 1205
Ama87I CYCGRG 1 cut(s) 260
Aor13HI TCCGGA 1 cut(s) 290
AoxI GGCC 2 cut(s) 57, 508
ApeKI GCWGC 3 cut(s) 33, 211, 307
ApoI RAATTY 3 cut(s) 1079, 1114, 1288
Asp718I GGTACC 1 cut(s) 264
AspA2I CCTAGG 1 cut(s) 695
AspLEI GCGC 2 cut(s) 236, 497
AspS9I GGNCC 2 cut(s) 57, 768
AsuC2I CCSGG 3 cut(s) 261, 262, 418
AsuHPI GGTGA 4 cut(s) 85, 151, 1155, 1223
AvaI CYCGRG 1 cut(s) 260
AvaII GGWCC 1 cut(s) 768
AvrII CCTAGG 1 cut(s) 695
BalI TGGCCA 1 cut(s) 510
BamHI GGATCC 1 cut(s) 287
BanI GGYRCC 2 cut(s) 233, 264
BanII GRGCYC 2 cut(s) 431, 528
BbsI GAAGAC 2 cut(s) 97, 375
Bbv12I GWGCWC 2 cut(s) 259, 431
BbvI GCAGC 3 cut(s) 45, 198, 294
BccI CCATC 3 cut(s) 180, 242, 485
BceAI ACGGC 2 cut(s) 30, 421
BcnI CCSGG 3 cut(s) 261, 262, 418
BcoDI GTCTC 2 cut(s) 869, 1145
BfaI CTAG 2 cut(s) 696, 1023
BfoI RGCGCY 1 cut(s) 237
BfuAI ACCTGC 1 cut(s) 719
BisI GCNGC 4 cut(s) 34, 186, 212, 308
BlnI CCTAGG 1 cut(s) 695
BlsI GCNGC 4 cut(s) 35, 187, 213, 309
Bme1390I CCNGG 3 cut(s) 261, 262, 418
Bme18I GGWCC 1 cut(s) 768
BmeT110I CYCGRG 1 cut(s) 260
BmgT120I GGNCC 2 cut(s) 57, 768
BmiI GGNNCC 5 cut(s) 235, 266, 289, 295, 852
BmrFI CCNGG 3 cut(s) 261, 262, 418
BmrI ACTGGG 2 cut(s) 79, 1161
BmsI GCATC 3 cut(s) 626, 715, 974
BmuI ACTGGG 2 cut(s) 79, 1161
BpiI GAAGAC 2 cut(s) 97, 375
BplI GAGNNNNNCTC 2 cut(s) 134, 166
BpmI CTGGAG 1 cut(s) 885
BpuMI CCSGG 3 cut(s) 261, 262, 418
BsaHI GRCGYC 3 cut(s) 156, 219, 234
BsaJI CCNNGG 3 cut(s) 22, 260, 695
BsaWI WCCGGW 1 cut(s) 290
Bsc4I CCNNNNNNNGG 4 cut(s) 152, 303, 533, 1155
Bse1I ACTGG 6 cut(s) 74, 467, 770, 902, 989, 1167
Bse3DI GCAATG 1 cut(s) 1129
BseAI TCCGGA 1 cut(s) 290
BseDI CCNNGG 3 cut(s) 22, 260, 695
BseGI GGATG 3 cut(s) 172, 589, 641
BseLI CCNNNNNNNGG 4 cut(s) 152, 303, 533, 1155
BseMI GCAATG 1 cut(s) 1129
BseMII CTCAG 1 cut(s) 446
BseNI ACTGG 6 cut(s) 74, 467, 770, 902, 989, 1167
BseXI GCAGC 3 cut(s) 45, 198, 294
BseYI CCCAGC 1 cut(s) 919
BsgI GTGCAG 1 cut(s) 197
Bsh1236I CGCG 2 cut(s) 497, 1306
BshFI GGCC 2 cut(s) 59, 510
BshNI GGYRCC 2 cut(s) 233, 264
BsiHKAI GWGCWC 2 cut(s) 259, 431
BsiHKCI CYCGRG 1 cut(s) 260
BsiSI CCGG 4 cut(s) 153, 261, 291, 417
BslFI GGGAC 1 cut(s) 570
BslI CCNNNNNNNGG 4 cut(s) 152, 303, 533, 1155
BsmAI GTCTC 2 cut(s) 869, 1145
BsmFI GGGAC 1 cut(s) 570
BsmI GAATGC 1 cut(s) 1159
BsnI GGCC 2 cut(s) 59, 510
BsoBI CYCGRG 1 cut(s) 260
Bsp1286I GDGCHC 3 cut(s) 259, 431, 528
Bsp13I TCCGGA 1 cut(s) 290
Bsp143I GATC 4 cut(s) 287, 397, 536, 970
Bsp68I TCGCGA 1 cut(s) 1306
BspACI CCGC 5 cut(s) 5, 31, 183, 186, 974
BspANI GGCC 2 cut(s) 59, 510
BspCNI CTCAG 1 cut(s) 445
BspEI TCCGGA 1 cut(s) 290
BspFNI CGCG 2 cut(s) 497, 1306
BspLI GGNNCC 5 cut(s) 235, 266, 289, 295, 852
BspMI ACCTGC 1 cut(s) 719
BspPI GGATC 5 cut(s) 282, 295, 405, 544, 965
BspT107I GGYRCC 2 cut(s) 233, 264
BsrDI GCAATG 1 cut(s) 1129
BsrI ACTGG 6 cut(s) 74, 467, 770, 902, 989, 1167
BssECI CCNNGG 3 cut(s) 22, 260, 695
BssMI GATC 4 cut(s) 287, 397, 536, 970
BssNI GRCGYC 3 cut(s) 156, 219, 234
BssT1I CCWWGG 1 cut(s) 695
Bst4CI ACNGT 5 cut(s) 97, 483, 488, 671, 709
Bst6I CTCTTC 1 cut(s) 870
BstACI GRCGYC 3 cut(s) 156, 219, 234
BstC8I GCNNGC 2 cut(s) 508, 998
BstDEI CTNAG 3 cut(s) 244, 432, 858
BstF5I GGATG 3 cut(s) 172, 589, 641
BstFNI CGCG 2 cut(s) 497, 1306
BstH2I RGCGCY 1 cut(s) 237
BstHHI GCGC 2 cut(s) 236, 497
BstKTI GATC 4 cut(s) 290, 400, 539, 973
BstMAI GTCTC 2 cut(s) 869, 1145
BstMBI GATC 4 cut(s) 287, 397, 536, 970
BstMWI GCNNNNNNNGC 6 cut(s) 86, 142, 240, 503, 620, 1023
BstSCI CCNGG 3 cut(s) 259, 260, 416
BstUI CGCG 2 cut(s) 497, 1306
BstV1I GCAGC 3 cut(s) 45, 198, 294
BstV2I GAAGAC 2 cut(s) 97, 375
BstX2I RGATCY 2 cut(s) 287, 970
BstXI CCANNNNNNTGG 1 cut(s) 394
BstYI RGATCY 2 cut(s) 287, 970
BsuRI GGCC 2 cut(s) 59, 510
BtsCI GGATG 3 cut(s) 172, 589, 641
BtsIMutI CAGTG 2 cut(s) 667, 909
BtuMI TCGCGA 1 cut(s) 1306
BveI ACCTGC 1 cut(s) 719
Cac8I GCNNGC 2 cut(s) 508, 998
CaiI CAGNNNCTG 1 cut(s) 1205
CfoI GCGC 2 cut(s) 236, 497
Cfr13I GGNCC 2 cut(s) 57, 768
Cfr9I CCCGGG 1 cut(s) 260
CseI GACGC 3 cut(s) 145, 227, 486
Csp6I GTAC 4 cut(s) 265, 391, 484, 1219
CviAII CATG 8 cut(s) 351, 394, 772, 977, 1119, 1140, 1155, 1263
CviQI GTAC 4 cut(s) 265, 391, 484, 1219
DdeI CTNAG 3 cut(s) 244, 432, 858
DinI GGCGCC 1 cut(s) 235
DpnI GATC 4 cut(s) 289, 399, 538, 972
DpnII GATC 4 cut(s) 287, 397, 536, 970
DrdI GACNNNNNNGTC 1 cut(s) 155
DseDI GACNNNNNNGTC 1 cut(s) 155
EaeI YGGCCR 1 cut(s) 508
Eam1104I CTCTTC 1 cut(s) 870
EarI CTCTTC 1 cut(s) 870
Ecl136II GAGCTC 1 cut(s) 429
Eco130I CCWWGG 1 cut(s) 695
Eco24I GRGCYC 2 cut(s) 431, 528
Eco47I GGWCC 1 cut(s) 768
Eco53kI GAGCTC 1 cut(s) 429
Eco57I CTGAAG 2 cut(s) 788, 981
Eco88I CYCGRG 1 cut(s) 260
EcoICRI GAGCTC 1 cut(s) 429
EcoRI GAATTC 1 cut(s) 1079
EcoT14I CCWWGG 1 cut(s) 695
EcoT22I ATGCAT 1 cut(s) 1072
EcoT38I GRGCYC 2 cut(s) 431, 528
EgeI GGCGCC 1 cut(s) 235
EheI GGCGCC 1 cut(s) 235
ErhI CCWWGG 1 cut(s) 695
FaeI CATG 8 cut(s) 354, 397, 775, 980, 1122, 1143, 1158, 1266
FalI AAGNNNNNCTT 4 cut(s) 533, 565, 851, 883
FaqI GGGAC 1 cut(s) 570
FatI CATG 8 cut(s) 350, 393, 771, 976, 1118, 1139, 1154, 1262
FauNDI CATATG 1 cut(s) 1284
FblI GTMKAC 1 cut(s) 198
Fnu4HI GCNGC 4 cut(s) 34, 186, 212, 308
FokI GGATG 3 cut(s) 159, 596, 648
FriOI GRGCYC 2 cut(s) 431, 528
Fsp4HI GCNGC 4 cut(s) 34, 186, 212, 308
FspBI CTAG 2 cut(s) 696, 1023
GlaI GCGC 2 cut(s) 235, 496
GluI GCNGC 4 cut(s) 34, 186, 212, 308
GsaI CCCAGC 1 cut(s) 923
GsuI CTGGAG 1 cut(s) 885
HaeII RGCGCY 1 cut(s) 237
HaeIII GGCC 2 cut(s) 59, 510
HapII CCGG 4 cut(s) 153, 261, 291, 417
HgaI GACGC 3 cut(s) 145, 227, 486
HhaI GCGC 2 cut(s) 236, 497
Hin1I GRCGYC 3 cut(s) 156, 219, 234
Hin1II CATG 8 cut(s) 354, 397, 775, 980, 1122, 1143, 1158, 1266
Hin6I GCGC 2 cut(s) 234, 495
HinP1I GCGC 2 cut(s) 234, 495
HincII GTYRAC 4 cut(s) 12, 199, 757, 916
HindII GTYRAC 4 cut(s) 12, 199, 757, 916
HindIII AAGCTT 1 cut(s) 542
HinfI GANTC 7 cut(s) 27, 149, 205, 347, 370, 701, 1202
HpaI GTTAAC 1 cut(s) 757
HpaII CCGG 4 cut(s) 153, 261, 291, 417
HphI GGTGA 4 cut(s) 85, 151, 1155, 1223
Hpy166II GTNNAC 4 cut(s) 12, 199, 757, 916
Hpy188I TCNGA 7 cut(s) 26, 275, 287, 363, 442, 970, 1150
Hpy8I GTNNAC 4 cut(s) 12, 199, 757, 916
Hpy99I CGWCG 3 cut(s) 143, 221, 1056
HpyAV CCTTC 3 cut(s) 100, 119, 178
HpyCH4III ACNGT 5 cut(s) 97, 483, 488, 671, 709
HpyCH4IV ACGT 1 cut(s) 891
HpyF10VI GCNNNNNNNGC 6 cut(s) 86, 142, 240, 503, 620, 1023
HpyF3I CTNAG 3 cut(s) 244, 432, 858
HpySE526I ACGT 1 cut(s) 891
Hsp92I GRCGYC 3 cut(s) 156, 219, 234
Hsp92II CATG 8 cut(s) 354, 397, 775, 980, 1122, 1143, 1158, 1266
HspAI GCGC 2 cut(s) 234, 495
KasI GGCGCC 1 cut(s) 233
Kpn2I TCCGGA 1 cut(s) 290
KpnI GGTACC 1 cut(s) 268
KspAI GTTAAC 1 cut(s) 757
Kzo9I GATC 4 cut(s) 287, 397, 536, 970
LmnI GCTCC 2 cut(s) 262, 293
Lsp1109I GCAGC 3 cut(s) 45, 198, 294
LweI GCATC 3 cut(s) 626, 715, 974
MaeI CTAG 2 cut(s) 696, 1023
MaeII ACGT 1 cut(s) 891
MaeIII GTNAC 1 cut(s) 157
MalI GATC 4 cut(s) 289, 399, 538, 972
MboI GATC 4 cut(s) 287, 397, 536, 970
MboII GAAGA 4 cut(s) 97, 375, 794, 887
MfeI CAATTG 2 cut(s) 597, 1194
MflI RGATCY 2 cut(s) 287, 970
MhlI GDGCHC 3 cut(s) 259, 431, 528
MlsI TGGCCA 1 cut(s) 510
MluCI AATT 9 cut(s) 301, 597, 1012, 1079, 1114, 1194, 1245, 1288, 1298
MluNI TGGCCA 1 cut(s) 510
Mly113I GGCGCC 1 cut(s) 234
MlyI GAGTC 6 cut(s) 21, 143, 199, 356, 364, 1211
MmeI TCCRAC 2 cut(s) 150, 200
MnlI CCTC 9 cut(s) 32, 95, 224, 313, 320, 677, 1090, 1094, 1301
Mox20I TGGCCA 1 cut(s) 510
Mph1103I ATGCAT 1 cut(s) 1072
MroI TCCGGA 1 cut(s) 290
MscI TGGCCA 1 cut(s) 510
MseI TTAA 4 cut(s) 459, 756, 885, 1224
MslI CAYNNNNRTG 3 cut(s) 672, 902, 1261
Msp20I TGGCCA 1 cut(s) 510
MspI CCGG 4 cut(s) 153, 261, 291, 417
MspR9I CCNGG 3 cut(s) 261, 262, 418
MunI CAATTG 2 cut(s) 597, 1194
Mva1269I GAATGC 1 cut(s) 1159
MvnI CGCG 2 cut(s) 497, 1306
MwoI GCNNNNNNNGC 6 cut(s) 86, 142, 240, 503, 620, 1023
NarI GGCGCC 1 cut(s) 234
NciI CCSGG 3 cut(s) 261, 262, 418
NdeI CATATG 1 cut(s) 1284
NdeII GATC 4 cut(s) 287, 397, 536, 970
NlaIII CATG 8 cut(s) 354, 397, 775, 980, 1122, 1143, 1158, 1266
NlaIV GGNNCC 5 cut(s) 235, 266, 289, 295, 852
NmuCI GTSAC 1 cut(s) 157
NruI TCGCGA 1 cut(s) 1306
NsiI ATGCAT 1 cut(s) 1072
OliI CACNNNNGTG 1 cut(s) 902
PcsI WCGNNNNNNNCGW 1 cut(s) 93
PctI GAATGC 1 cut(s) 1159
PfeI GAWTC 1 cut(s) 701
PflMI CCANNNNNTGG 1 cut(s) 303
PfoI TCCNGGA 1 cut(s) 416
PkrI GCNGC 4 cut(s) 35, 187, 213, 309
PleI GAGTC 6 cut(s) 21, 143, 199, 355, 364, 1210
PluTI GGCGCC 1 cut(s) 237
PpsI GAGTC 6 cut(s) 21, 143, 199, 355, 364, 1210
PsiI TTATAA 1 cut(s) 1112
Psp124BI GAGCTC 1 cut(s) 431
PspFI CCCAGC 1 cut(s) 919
PspN4I GGNNCC 5 cut(s) 235, 266, 289, 295, 852
PspPI GGNCC 2 cut(s) 57, 768
PstNI CAGNNNCTG 1 cut(s) 1205
PsuI RGATCY 2 cut(s) 287, 970
RruI TCGCGA 1 cut(s) 1306
RsaI GTAC 4 cut(s) 266, 392, 485, 1220
RsaNI GTAC 4 cut(s) 265, 391, 484, 1219
RseI CAYNNNNRTG 3 cut(s) 672, 902, 1261
SacI GAGCTC 1 cut(s) 431
SalI GTCGAC 1 cut(s) 197
SaqAI TTAA 4 cut(s) 459, 756, 885, 1224
SatI GCNGC 4 cut(s) 34, 186, 212, 308
Sau3AI GATC 4 cut(s) 287, 397, 536, 970
Sau96I GGNCC 2 cut(s) 57, 768
SchI GAGTC 6 cut(s) 21, 143, 199, 356, 364, 1211
ScrFI CCNGG 3 cut(s) 261, 262, 418
SduI GDGCHC 3 cut(s) 259, 431, 528
SfaNI GCATC 3 cut(s) 626, 715, 974
SfoI GGCGCC 1 cut(s) 235
SinI GGWCC 1 cut(s) 768
SmaI CCCGGG 1 cut(s) 262
SmiMI CAYNNNNRTG 3 cut(s) 672, 902, 1261
Sse9I AATT 9 cut(s) 301, 597, 1012, 1079, 1114, 1194, 1245, 1288, 1298
SsiI CCGC 5 cut(s) 5, 31, 183, 186, 974
SspDI GGCGCC 1 cut(s) 233
SspMI CTAG 2 cut(s) 696, 1023
SstI GAGCTC 1 cut(s) 431
StyD4I CCNGG 3 cut(s) 259, 260, 416
StyI CCWWGG 1 cut(s) 695
TaaI ACNGT 5 cut(s) 97, 483, 488, 671, 709
TaiI ACGT 1 cut(s) 894
TaqI TCGA 4 cut(s) 198, 682, 741, 1228
TasI AATT 9 cut(s) 301, 597, 1012, 1079, 1114, 1194, 1245, 1288, 1298
TatI WGTACW 1 cut(s) 390
TauI GCSGC 1 cut(s) 188
TfiI GAWTC 1 cut(s) 701
Tru1I TTAA 4 cut(s) 459, 756, 885, 1224
Tru9I TTAA 4 cut(s) 459, 756, 885, 1224
TscAI CASTG 2 cut(s) 674, 909
TseFI GTSAC 1 cut(s) 157
TseI GCWGC 3 cut(s) 33, 211, 307
Tsp45I GTSAC 1 cut(s) 157
TspDTI ATGAA 4 cut(s) 600, 1107, 1118, 1301
TspGWI ACGGA 1 cut(s) 1040
TspMI CCCGGG 1 cut(s) 260
TspRI CASTG 2 cut(s) 674, 909
Van91I CCANNNNNTGG 1 cut(s) 303
VpaK11BI GGWCC 1 cut(s) 768
XapI RAATTY 3 cut(s) 1079, 1114, 1288
XmaI CCCGGG 1 cut(s) 260
XmaJI CCTAGG 1 cut(s) 695
XmiI GTMKAC 1 cut(s) 198
XspI CTAG 2 cut(s) 696, 1023
Zsp2I ATGCAT 1 cut(s) 1072
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.