Rroxscaffold_6G00403990

Hydrolyzes acetyl esters in homogalacturonan regions of pectin. In type I primary cell wall, galacturonic acid residues of pectin can be acetylated at the O-2 and O-3 positions. Decreasing the degree of acetylation of pectin gels in vitro alters their physical properties

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000006
OG0001587
Physical Location & Seq
Forward (+)
26424145 .. 26428005
3861 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_6G00403990.1

Sequence Viewer

Length: 1023 bp
ATGAACCCCCGCTCGATCGGACCTGACAAGAAGCCTCTTTCAGCAGGACCACAACCCGGAAGTCCCGGGTTCCGAGGAGCCGGCTCCTCAATCGCCGCCCTCGCCTTTACCGTCATTCTCTTCCTTCTCACTCTCATCTCCAACTCTTGGCTCCCCGAGCCTGACTCCGCCGTCACCAACCCCTTCCATCCAACCACCGCCGCCGATTTGGTCGACTTGACTCTGCTGCACGACGCCGAACTTGACTCTGCGCCCATGGGAGTGCTCCCGGGGTGGGGTTGGTGCAATACAATAGAGTCATGTTCTTTTCGGAAAAGGACTCCATTAGGGTCTTCCAAGTACATGGATCGTAAGGTTCAGTTTTCCGGGATTTTGAGCTCTCAGCGGTCAGAAAATCCTGGAACAAAGTCAAAATACGGTACAGTGACGGCGCATCTTTTGCTGGCCACCCCAAAAATGAGCCGAAGGGGACAGCTTATCTGGGAAGCACCTATGGATGAACTCTTGTCAATTGGCTTGTCAAAGGCGAAACAGGCGCTTCTTTCTGCATGCTCTGCCGGCGGGTTGGCAAGTCTTATACACTGTGATGACTTTCGAGGTCTTTTACCTAGGGATTCAATTGCCAAATGTCTTGCTGATGCAGGTTATGCTCTAATCTTACTTCCTTCTGTAATCTTTACTTGTACTCCGTTGTATGATCTTTTGCTAGAAGATTTAGCAAAGAGTTTGCACAAGGATTGTGTTGCAAGAAAGGAACCAGCTAAGTGTCTCTTTCCTGAAGAAATTATTAAACACGTTAGCACTCCAGTGTTCCTTGTCAACCCAGCTTATGATTTTTGGCAGATACAACATATCTTGATACCTGAAGCAGCAGATCCGCATGGCTACTGGAAAAAGTGCAAGCTGAACATCTATAATTGCAATCCTAGCCAGATTGAAATACTGCAAGCCCTGCAAATTGTAATCAGCAGGAAATTTAGTGGGACTGACTGTTCTGGAACCCATGTAACAGATGCACTTTAA

Protein Analysis

340

Amino Acids

36.94

Weight (kDa)

7.55

Isoelectric Point (pI)

49.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PAE PF03283 89 - 133 1.7e-11 Pectinacetylesterase
PAE PF03283 152 - 326 4e-50 Pectinacetylesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 170
Acc36I ACCTGC 1 cut(s) 632
AccB7I CCANNNNNTGG 1 cut(s) 147
AccBSI CCGCTC 1 cut(s) 12
AccI GTMKAC 1 cut(s) 213
AciI CCGC 8 cut(s) 10, 96, 168, 198, 201, 385, 561, 878
AclWI GGATC 2 cut(s) 354, 869
AcoI YGGCCR 1 cut(s) 444
AcsI RAATTY 1 cut(s) 974
AcuI CTGAAG 2 cut(s) 798, 885
AcyI GRCGYC 1 cut(s) 234
AfaI GTAC 3 cut(s) 341, 421, 685
AfiI CCNNNNNNNGG 4 cut(s) 56, 147, 274, 275
AflIII ACRYGT 1 cut(s) 793
AgsI TTSAA 2 cut(s) 618, 938
AjnI CCWGG 1 cut(s) 397
AleI CACNNNNGTG 1 cut(s) 806
AloI GAACNNNNNNTCC 2 cut(s) 976, 1008
AluBI AGCT 5 cut(s) 378, 475, 761, 827, 904
AluI AGCT 5 cut(s) 378, 475, 761, 827, 904
Alw21I GWGCWC 2 cut(s) 267, 380
Alw26I GTCTC 1 cut(s) 773
AlwI GGATC 2 cut(s) 354, 869
Ama87I CYCGRG 3 cut(s) 65, 155, 268
AoxI GGCC 1 cut(s) 444
ApeKI GCWGC 2 cut(s) 226, 869
ApoI RAATTY 1 cut(s) 974
AspA2I CCTAGG 1 cut(s) 608
AspLEI GCGC 3 cut(s) 253, 433, 538
AspS9I GGNCC 2 cut(s) 20, 47
AsuC2I CCSGG 6 cut(s) 57, 66, 67, 269, 270, 367
AsuHPI GGTGA 1 cut(s) 166
AvaI CYCGRG 3 cut(s) 65, 155, 268
AvaII GGWCC 2 cut(s) 20, 47
AvrII CCTAGG 1 cut(s) 608
BalI TGGCCA 1 cut(s) 446
BanII GRGCYC 1 cut(s) 380
BbsI GAAGAC 1 cut(s) 324
Bbv12I GWGCWC 2 cut(s) 267, 380
BbvI GCAGC 2 cut(s) 213, 881
BccI CCATC 1 cut(s) 195
BceAI ACGGC 2 cut(s) 155, 444
BciT130I CCWGG 1 cut(s) 399
BcnI CCSGG 6 cut(s) 57, 66, 67, 269, 270, 367
BcoDI GTCTC 1 cut(s) 773
BfaI CTAG 3 cut(s) 609, 707, 927
BfoI RGCGCY 1 cut(s) 539
BfuAI ACCTGC 1 cut(s) 632
BisI GCNGC 4 cut(s) 96, 201, 227, 870
BlnI CCTAGG 1 cut(s) 608
BlsI GCNGC 4 cut(s) 97, 202, 228, 871
Bme1390I CCNGG 7 cut(s) 57, 66, 67, 269, 270, 367, 399
Bme18I GGWCC 2 cut(s) 20, 47
BmeT110I CYCGRG 3 cut(s) 65, 155, 268
BmgT120I GGNCC 2 cut(s) 20, 47
BmiI GGNNCC 6 cut(s) 71, 79, 85, 152, 756, 1000
BmrFI CCNGG 7 cut(s) 57, 66, 67, 269, 270, 367, 399
BmsI GCATC 3 cut(s) 442, 628, 1003
BpiI GAAGAC 1 cut(s) 324
BplI GAGNNNNNCTC 2 cut(s) 149, 181
BpmI CTGGAG 1 cut(s) 789
BpuMI CCSGG 6 cut(s) 57, 66, 67, 269, 270, 367
BsaHI GRCGYC 1 cut(s) 234
BsaJI CCNNGG 6 cut(s) 65, 73, 255, 268, 269, 608
BsaXI ACNNNNNCTCC 2 cut(s) 670, 700
Bsc4I CCNNNNNNNGG 4 cut(s) 56, 147, 274, 275
Bse118I RCCGGY 2 cut(s) 80, 557
Bse1I ACTGG 2 cut(s) 806, 893
BseBI CCWGG 1 cut(s) 399
BseDI CCNNGG 6 cut(s) 65, 73, 255, 268, 269, 608
BseGI GGATG 2 cut(s) 187, 502
BseLI CCNNNNNNNGG 4 cut(s) 56, 147, 274, 275
BseMII CTCAG 1 cut(s) 395
BseNI ACTGG 2 cut(s) 806, 893
BseRI GAGGAG 2 cut(s) 76, 90
BseXI GCAGC 2 cut(s) 213, 881
BseYI CCCAGC 1 cut(s) 823
BsgI GTGCAG 1 cut(s) 212
Bsh1285I CGRYCG 1 cut(s) 18
BshFI GGCC 1 cut(s) 446
BsiEI CGRYCG 1 cut(s) 18
BsiHKAI GWGCWC 2 cut(s) 267, 380
BsiHKCI CYCGRG 3 cut(s) 65, 155, 268
BsiSI CCGG 6 cut(s) 57, 66, 81, 269, 366, 558
BslFI GGGAC 3 cut(s) 48, 483, 997
BslI CCNNNNNNNGG 4 cut(s) 56, 147, 274, 275
BsmAI GTCTC 1 cut(s) 773
BsmFI GGGAC 3 cut(s) 48, 483, 997
BsnI GGCC 1 cut(s) 446
BsoBI CYCGRG 3 cut(s) 65, 155, 268
Bsp1286I GDGCHC 2 cut(s) 267, 380
Bsp143I GATC 4 cut(s) 15, 346, 697, 874
Bsp19I CCATGG 1 cut(s) 255
BspACI CCGC 8 cut(s) 10, 96, 168, 198, 201, 385, 561, 878
BspANI GGCC 1 cut(s) 446
BspCNI CTCAG 1 cut(s) 394
BspLI GGNNCC 6 cut(s) 71, 79, 85, 152, 756, 1000
BspMI ACCTGC 1 cut(s) 632
BspPI GGATC 2 cut(s) 354, 869
BsrBI CCGCTC 1 cut(s) 12
BsrFI RCCGGY 2 cut(s) 80, 557
BsrI ACTGG 2 cut(s) 806, 893
BssAI RCCGGY 2 cut(s) 80, 557
BssECI CCNNGG 6 cut(s) 65, 73, 255, 268, 269, 608
BssMI GATC 4 cut(s) 15, 346, 697, 874
BssNI GRCGYC 1 cut(s) 234
BssT1I CCWWGG 2 cut(s) 255, 608
Bst2UI CCWGG 1 cut(s) 399
Bst4CI ACNGT 5 cut(s) 112, 419, 424, 584, 992
Bst6I CTCTTC 1 cut(s) 125
BstACI GRCGYC 1 cut(s) 234
BstAPI GCANNNNNTGC 4 cut(s) 439, 554, 647, 952
BstC8I GCNNGC 6 cut(s) 82, 444, 550, 559, 902, 948
BstDEI CTNAG 2 cut(s) 381, 762
BstDSI CCRYGG 1 cut(s) 255
BstF5I GGATG 2 cut(s) 187, 502
BstH2I RGCGCY 1 cut(s) 539
BstHHI GCGC 3 cut(s) 253, 433, 538
BstKTI GATC 4 cut(s) 18, 349, 700, 877
BstMAI GTCTC 1 cut(s) 773
BstMBI GATC 4 cut(s) 15, 346, 697, 874
BstMCI CGRYCG 1 cut(s) 18
BstMWI GCNNNNNNNGC 9 cut(s) 101, 157, 439, 533, 554, 558, 647, 927, 952
BstNI CCWGG 1 cut(s) 399
BstNSI RCATGY 1 cut(s) 552
BstSCI CCNGG 7 cut(s) 55, 64, 65, 267, 268, 365, 397
BstV1I GCAGC 2 cut(s) 213, 881
BstV2I GAAGAC 1 cut(s) 324
BstX2I RGATCY 1 cut(s) 874
BstXI CCANNNNNNTGG 1 cut(s) 343
BstYI RGATCY 1 cut(s) 874
BsuRI GGCC 1 cut(s) 446
BtgI CCRYGG 1 cut(s) 255
BtsCI GGATG 2 cut(s) 187, 502
BtsIMutI CAGTG 3 cut(s) 429, 580, 813
BveI ACCTGC 1 cut(s) 632
Cac8I GCNNGC 6 cut(s) 82, 444, 550, 559, 902, 948
CfoI GCGC 3 cut(s) 253, 433, 538
Cfr10I RCCGGY 2 cut(s) 80, 557
Cfr13I GGNCC 2 cut(s) 20, 47
Cfr9I CCCGGG 2 cut(s) 65, 268
CseI GACGC 1 cut(s) 242
Csp6I GTAC 3 cut(s) 340, 420, 684
CviAII CATG 6 cut(s) 256, 300, 343, 549, 881, 1004
CviQI GTAC 3 cut(s) 340, 420, 684
DdeI CTNAG 2 cut(s) 381, 762
DpnI GATC 4 cut(s) 17, 348, 699, 876
DpnII GATC 4 cut(s) 15, 346, 697, 874
DrdI GACNNNNNNGTC 1 cut(s) 170
DseDI GACNNNNNNGTC 1 cut(s) 170
EaeI YGGCCR 1 cut(s) 444
Eam1104I CTCTTC 1 cut(s) 125
EarI CTCTTC 1 cut(s) 125
EciI GGCGGA 1 cut(s) 157
Ecl136II GAGCTC 1 cut(s) 378
Eco130I CCWWGG 2 cut(s) 255, 608
Eco24I GRGCYC 1 cut(s) 380
Eco47I GGWCC 2 cut(s) 20, 47
Eco53kI GAGCTC 1 cut(s) 378
Eco57I CTGAAG 2 cut(s) 798, 885
Eco88I CYCGRG 3 cut(s) 65, 155, 268
EcoICRI GAGCTC 1 cut(s) 378
EcoRII CCWGG 1 cut(s) 397
EcoT14I CCWWGG 2 cut(s) 255, 608
EcoT38I GRGCYC 1 cut(s) 380
ErhI CCWWGG 2 cut(s) 255, 608
FaeI CATG 6 cut(s) 259, 303, 346, 552, 884, 1007
FalI AAGNNNNNCTT 2 cut(s) 755, 787
FaqI GGGAC 3 cut(s) 48, 483, 997
FatI CATG 6 cut(s) 255, 299, 342, 548, 880, 1003
FauI CCCGC 2 cut(s) 17, 554
FblI GTMKAC 1 cut(s) 213
Fnu4HI GCNGC 4 cut(s) 96, 201, 227, 870
FokI GGATG 2 cut(s) 174, 509
FriOI GRGCYC 1 cut(s) 380
Fsp4HI GCNGC 4 cut(s) 96, 201, 227, 870
FspBI CTAG 3 cut(s) 609, 707, 927
GlaI GCGC 3 cut(s) 252, 432, 537
GluI GCNGC 4 cut(s) 96, 201, 227, 870
GsaI CCCAGC 1 cut(s) 827
GsuI CTGGAG 1 cut(s) 789
HaeII RGCGCY 1 cut(s) 539
HaeIII GGCC 1 cut(s) 446
HapII CCGG 6 cut(s) 57, 66, 81, 269, 366, 558
HgaI GACGC 1 cut(s) 242
HhaI GCGC 3 cut(s) 253, 433, 538
Hin1I GRCGYC 1 cut(s) 234
Hin1II CATG 6 cut(s) 259, 303, 346, 552, 884, 1007
Hin6I GCGC 3 cut(s) 251, 431, 536
HinP1I GCGC 3 cut(s) 251, 431, 536
HincII GTYRAC 2 cut(s) 214, 820
HindII GTYRAC 2 cut(s) 214, 820
HinfI GANTC 6 cut(s) 164, 220, 245, 296, 319, 614
HpaII CCGG 6 cut(s) 57, 66, 81, 269, 366, 558
HphI GGTGA 1 cut(s) 166
Hpy166II GTNNAC 2 cut(s) 214, 820
Hpy188I TCNGA 4 cut(s) 20, 74, 312, 391
Hpy188III TCNNGA 3 cut(s) 776, 856, 996
Hpy8I GTNNAC 2 cut(s) 214, 820
Hpy99I CGWCG 1 cut(s) 236
HpyAV CCTTC 4 cut(s) 134, 193, 459, 675
HpyCH4III ACNGT 5 cut(s) 112, 419, 424, 584, 992
HpyCH4IV ACGT 1 cut(s) 795
HpyF10VI GCNNNNNNNGC 9 cut(s) 101, 157, 439, 533, 554, 558, 647, 927, 952
HpyF3I CTNAG 2 cut(s) 381, 762
HpySE526I ACGT 1 cut(s) 795
Hsp92I GRCGYC 1 cut(s) 234
Hsp92II CATG 6 cut(s) 259, 303, 346, 552, 884, 1007
HspAI GCGC 3 cut(s) 251, 431, 536
KroI GCCGGC 2 cut(s) 80, 557
KroNI GCCGGC 2 cut(s) 82, 559
Kzo9I GATC 4 cut(s) 15, 346, 697, 874
LmnI GCTCC 4 cut(s) 77, 89, 156, 270
Lsp1109I GCAGC 2 cut(s) 213, 881
LweI GCATC 3 cut(s) 442, 628, 1003
MaeI CTAG 3 cut(s) 609, 707, 927
MaeII ACGT 1 cut(s) 795
MaeIII GTNAC 3 cut(s) 172, 424, 1006
MalI GATC 4 cut(s) 17, 348, 699, 876
MbiI CCGCTC 1 cut(s) 12
MboI GATC 4 cut(s) 15, 346, 697, 874
MboII GAAGA 4 cut(s) 112, 324, 722, 791
MfeI CAATTG 2 cut(s) 510, 618
MflI RGATCY 1 cut(s) 874
MhlI GDGCHC 2 cut(s) 267, 380
MlsI TGGCCA 1 cut(s) 446
MluCI AATT 6 cut(s) 510, 618, 783, 916, 957, 974
MluNI TGGCCA 1 cut(s) 446
MlyI GAGTC 5 cut(s) 158, 214, 239, 305, 313
MmeI TCCRAC 2 cut(s) 165, 215
MnlI CCTC 5 cut(s) 45, 68, 97, 110, 590
Mox20I TGGCCA 1 cut(s) 446
MroNI GCCGGC 2 cut(s) 80, 557
MscI TGGCCA 1 cut(s) 446
MseI TTAA 2 cut(s) 789, 1021
MslI CAYNNNNRTG 3 cut(s) 260, 585, 806
Msp20I TGGCCA 1 cut(s) 446
MspA1I CMGCKG 1 cut(s) 385
MspI CCGG 6 cut(s) 57, 66, 81, 269, 366, 558
MspR9I CCNGG 7 cut(s) 57, 66, 67, 269, 270, 367, 399
MunI CAATTG 2 cut(s) 510, 618
MvaI CCWGG 1 cut(s) 399
MwoI GCNNNNNNNGC 9 cut(s) 101, 157, 439, 533, 554, 558, 647, 927, 952
NaeI GCCGGC 2 cut(s) 82, 559
NciI CCSGG 6 cut(s) 57, 66, 67, 269, 270, 367
NcoI CCATGG 1 cut(s) 255
NdeII GATC 4 cut(s) 15, 346, 697, 874
NgoMIV GCCGGC 2 cut(s) 80, 557
NlaIII CATG 6 cut(s) 259, 303, 346, 552, 884, 1007
NlaIV GGNNCC 6 cut(s) 71, 79, 85, 152, 756, 1000
NmuCI GTSAC 2 cut(s) 172, 424
NspI RCATGY 1 cut(s) 552
OliI CACNNNNGTG 1 cut(s) 806
PaeI GCATGC 1 cut(s) 552
PcsI WCGNNNNNNNCGW 1 cut(s) 108
PdiI GCCGGC 2 cut(s) 82, 559
PfeI GAWTC 1 cut(s) 614
PflMI CCANNNNNTGG 1 cut(s) 147
PfoI TCCNGGA 2 cut(s) 365, 397
PkrI GCNGC 4 cut(s) 97, 202, 228, 871
Ple19I CGATCG 1 cut(s) 18
PleI GAGTC 5 cut(s) 158, 214, 239, 304, 313
PpsI GAGTC 5 cut(s) 158, 214, 239, 304, 313
Psp124BI GAGCTC 1 cut(s) 380
Psp6I CCWGG 1 cut(s) 397
PspFI CCCAGC 1 cut(s) 823
PspGI CCWGG 1 cut(s) 397
PspN4I GGNNCC 6 cut(s) 71, 79, 85, 152, 756, 1000
PspPI GGNCC 2 cut(s) 20, 47
PsuI RGATCY 1 cut(s) 874
PvuI CGATCG 1 cut(s) 18
RsaI GTAC 3 cut(s) 341, 421, 685
RsaNI GTAC 3 cut(s) 340, 420, 684
RseI CAYNNNNRTG 3 cut(s) 260, 585, 806
SacI GAGCTC 1 cut(s) 380
SalI GTCGAC 1 cut(s) 212
SaqAI TTAA 2 cut(s) 789, 1021
SatI GCNGC 4 cut(s) 96, 201, 227, 870
Sau3AI GATC 4 cut(s) 15, 346, 697, 874
Sau96I GGNCC 2 cut(s) 20, 47
SchI GAGTC 5 cut(s) 158, 214, 239, 305, 313
ScrFI CCNGG 7 cut(s) 57, 66, 67, 269, 270, 367, 399
SduI GDGCHC 2 cut(s) 267, 380
SfaNI GCATC 3 cut(s) 442, 628, 1003
SinI GGWCC 2 cut(s) 20, 47
SmaI CCCGGG 2 cut(s) 67, 270
SmiMI CAYNNNNRTG 3 cut(s) 260, 585, 806
SphI GCATGC 1 cut(s) 552
Sse9I AATT 6 cut(s) 510, 618, 783, 916, 957, 974
SsiI CCGC 8 cut(s) 10, 96, 168, 198, 201, 385, 561, 878
SspMI CTAG 3 cut(s) 609, 707, 927
SstI GAGCTC 1 cut(s) 380
StyD4I CCNGG 7 cut(s) 55, 64, 65, 267, 268, 365, 397
StyI CCWWGG 2 cut(s) 255, 608
TaaI ACNGT 5 cut(s) 112, 419, 424, 584, 992
TaiI ACGT 1 cut(s) 798
TaqI TCGA 3 cut(s) 14, 213, 595
TasI AATT 6 cut(s) 510, 618, 783, 916, 957, 974
TatI WGTACW 2 cut(s) 339, 683
TauI GCSGC 2 cut(s) 98, 203
TfiI GAWTC 1 cut(s) 614
Tru1I TTAA 2 cut(s) 789, 1021
Tru9I TTAA 2 cut(s) 789, 1021
TscAI CASTG 3 cut(s) 429, 587, 813
TseFI GTSAC 2 cut(s) 172, 424
TseI GCWGC 2 cut(s) 226, 869
Tsp45I GTSAC 2 cut(s) 172, 424
TspDTI ATGAA 2 cut(s) 17, 513
TspGWI ACGGA 1 cut(s) 678
TspMI CCCGGG 2 cut(s) 65, 268
TspRI CASTG 3 cut(s) 429, 587, 813
Van91I CCANNNNNTGG 1 cut(s) 147
VpaK11BI GGWCC 2 cut(s) 20, 47
XapI RAATTY 1 cut(s) 974
XceI RCATGY 1 cut(s) 552
XmaI CCCGGG 2 cut(s) 65, 268
XmaJI CCTAGG 1 cut(s) 608
XmiI GTMKAC 1 cut(s) 213
XspI CTAG 3 cut(s) 609, 707, 927
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.