Prupe.6G254500_v2.0.a1

Hydrolyzes acetyl esters in homogalacturonan regions of pectin. In type I primary cell wall, galacturonic acid residues of pectin can be acetylated at the O-2 and O-3 positions. Decreasing the degree of acetylation of pectin gels in vitro alters their physical properties

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp06
OG0001587
Physical Location & Seq
Forward (+)
24763204 .. 24767821
4618 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.6G254500.3

Sequence Viewer

Length: 1314 bp
ATGGCGGGGTCAATGCCAAATCCTAGGCTTCGCAGCCTGCTATGGTGGAGACGGTGGGCCAAAAAGGACTGGGCAATCGCAGCCGTCGGATTTACCGTTGTTGCCTTCGCTCTCACTCTCCTCTCCAACTCTTGGCGCGATGAACCAGACTCCGATATCACCCGCCCATTTCATCCCATCACCGCCTCCGATTTGGTCGAATTAACATTGTTGCAAAACGCTAAAGCTAGAGGCGCCCTATGTTTAGATGGAAGTGCGCCTGGCTACCATTTCAAAAAGGGCTTTGGATCTGGAGCTAACAATTGGCTGCTTCACATTGAGGGTGGAGGTTGGTGCAATACAATGGAATCTTGTTCATGGCGTAAAGGGACCTCATTAGGATCTTCCAAATACATGGACCATAAAGTTCCCTTTTCTGGGATTTTAAGTTCCCACCCATCAGAAAATCCTGAGTTCTTTAACTGGAACAAAGTCAAAATCCGGTACTGTGATGGCGCATCCTTAGCTGGCCACCCAGAAAATGACCTGAAGAATGGATCAGCACTTTTCTTTAGGGGCCAGCTCATCTGGGAAGCAGTTATGGATGAACTCTTGTCAGTTGGCTTGTCAAAAGCCAAACAGGCCCTTCTTTCGGGATGCTCTGCTGGTGGGTTGGCGACTCTTATACATTGTGATGACTTTCGACGCCTTTTGCCTACGGATGCAACTGTCAAATGTCTTGCTGATGCAGGTTTTTTTCTTGATGAGAAAGATGTTCTCCAACATCGGACCATGAGAGCTTTCTATCATGATGTTGTCCTCCTTCAGGGTTTAGCAAAAAGTTTGCACCAGGATTGTGTTACAAGAATGGAACCCTCTAAGTGTCTCTTTCCTGAAGAAATTATTAAAAACATTAACACCCCAGTGTTCCTAGTCAACCCAGCTTACGATTTTTGGCAGATACAACATATCTTGATACCTGAAGCATCAGATCCACATGGCTATTGGCAAAAGTGCAAATTGAACATTTACAATTGCAATCCTAGCCAGCTTGAAATACTACAAGGGTTCCGCGGTTCGATGCTGAAGGCACTGAATGAATTCCGAAAAAATAAGGAGGGAGGAATGTTTATAAATTCATGTTTTATTCATTGCCAGACGTGGATAACTGAGACATGGCATTCACCAAGTTCTCCACGAATAAACAATAAGACAATTGCAGAGTCTGTGGGCAATTGGTACTTTAGTCGGAATGTCGTAAAGCAAATCGACTGCCCATTTCCATGCAATCCCACCTGCTATCATATGAATTTCACTGTTTTCTCACGAGGTTGA

Protein Analysis

438

Amino Acids

49.26

Weight (kDa)

8.41

Isoelectric Point (pI)

46.57

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1112
AarI CACCTGC 1 cut(s) 1283
Acc36I ACCTGC 2 cut(s) 719, 1283
AccB1I GGYRCC 1 cut(s) 233
AccB7I CCANNNNNTGG 1 cut(s) 132
AccII CGCG 2 cut(s) 138, 1053
AciI CCGC 5 cut(s) 5, 163, 183, 1051, 1053
AclWI GGATC 4 cut(s) 295, 388, 544, 965
AcoI YGGCCR 1 cut(s) 508
AcsI RAATTY 3 cut(s) 1079, 1114, 1288
AcuI CTGAAG 5 cut(s) 548, 788, 894, 981, 1085
AcyI GRCGYC 2 cut(s) 234, 685
AfaI GTAC 2 cut(s) 485, 1220
AfiI CCNNNNNNNGG 5 cut(s) 132, 416, 417, 631, 805
AgsI TTSAA 3 cut(s) 274, 1003, 1034
AjiI CACGTC 1 cut(s) 1140
AjnI CCWGG 2 cut(s) 259, 828
AleI CACNNNNGTG 1 cut(s) 902
AloI GAACNNNNNNTCC 2 cut(s) 412, 444
AluBI AGCT 7 cut(s) 227, 296, 506, 562, 779, 923, 1030
AluI AGCT 7 cut(s) 227, 296, 506, 562, 779, 923, 1030
Alw26I GTCTC 3 cut(s) 43, 869, 1145
AlwI GGATC 4 cut(s) 295, 388, 544, 965
AlwNI CAGNNNCTG 1 cut(s) 1205
AoxI GGCC 4 cut(s) 57, 508, 556, 621
ApeKI GCWGC 3 cut(s) 33, 80, 307
ApoI RAATTY 3 cut(s) 1079, 1114, 1288
Asp700I GAANNNNTTC 1 cut(s) 1079
AspA2I CCTAGG 1 cut(s) 23
AspLEI GCGC 4 cut(s) 138, 236, 259, 497
AspS9I GGNCC 6 cut(s) 57, 369, 397, 556, 622, 768
AsuHPI GGTGA 3 cut(s) 151, 172, 1155
AvaII GGWCC 3 cut(s) 369, 397, 768
AvrII CCTAGG 1 cut(s) 23
BaeI ACNNNNGTAYC 2 cut(s) 1210, 1243
BalI TGGCCA 1 cut(s) 510
BanI GGYRCC 1 cut(s) 233
BauI CACGAG 1 cut(s) 1305
BbvI GCAGC 3 cut(s) 45, 92, 294
BccI CCATC 4 cut(s) 185, 242, 445, 485
BceAI ACGGC 1 cut(s) 68
BciT130I CCWGG 2 cut(s) 261, 830
BcoDI GTCTC 3 cut(s) 43, 869, 1145
BfaI CTAG 4 cut(s) 24, 228, 911, 1023
BfoI RGCGCY 1 cut(s) 237
BfuAI ACCTGC 2 cut(s) 719, 1283
BglI GCCNNNNNGGC 1 cut(s) 620
BisI GCNGC 3 cut(s) 34, 81, 308
BlnI CCTAGG 1 cut(s) 23
BlsI GCNGC 3 cut(s) 35, 82, 309
Bme1390I CCNGG 2 cut(s) 261, 830
Bme18I GGWCC 3 cut(s) 369, 397, 768
BmgBI CACGTC 1 cut(s) 1140
BmgT120I GGNCC 6 cut(s) 57, 369, 397, 556, 622, 768
BmiI GGNNCC 5 cut(s) 235, 370, 557, 852, 1049
BmrFI CCNGG 2 cut(s) 261, 830
BmrI ACTGGG 2 cut(s) 79, 896
BmsI GCATC 6 cut(s) 506, 626, 691, 715, 974, 1050
BmuI ACTGGG 2 cut(s) 79, 896
BpmI CTGGAG 1 cut(s) 312
Bpu10I CCTNAGC 1 cut(s) 502
BsaHI GRCGYC 2 cut(s) 234, 685
BsaJI CCNNGG 2 cut(s) 23, 1051
BsaWI WCCGGW 1 cut(s) 480
Bsc4I CCNNNNNNNGG 5 cut(s) 132, 416, 417, 631, 805
Bse1I ACTGG 3 cut(s) 74, 467, 902
Bse3DI GCAATG 1 cut(s) 1129
BseBI CCWGG 2 cut(s) 261, 830
BseDI CCNNGG 2 cut(s) 23, 1051
BseGI GGATG 5 cut(s) 172, 497, 589, 641, 706
BseLI CCNNNNNNNGG 5 cut(s) 132, 416, 417, 631, 805
BseMI GCAATG 1 cut(s) 1129
BseMII CTCAG 2 cut(s) 441, 1140
BseNI ACTGG 3 cut(s) 74, 467, 902
BseRI GAGGAG 1 cut(s) 110
BseXI GCAGC 3 cut(s) 45, 92, 294
BseYI CCCAGC 1 cut(s) 919
Bsh1236I CGCG 2 cut(s) 138, 1053
BshFI GGCC 4 cut(s) 59, 510, 558, 623
BshNI GGYRCC 1 cut(s) 233
BsiSI CCGG 1 cut(s) 481
BslFI GGGAC 1 cut(s) 382
BslI CCNNNNNNNGG 5 cut(s) 132, 416, 417, 631, 805
BsmAI GTCTC 3 cut(s) 43, 869, 1145
BsmBI CGTCTC 1 cut(s) 43
BsmFI GGGAC 1 cut(s) 382
BsmI GAATGC 1 cut(s) 1159
BsnI GGCC 4 cut(s) 59, 510, 558, 623
Bsp143I GATC 4 cut(s) 287, 380, 536, 970
BspACI CCGC 5 cut(s) 5, 163, 183, 1051, 1053
BspANI GGCC 4 cut(s) 59, 510, 558, 623
BspCNI CTCAG 2 cut(s) 442, 1141
BspFNI CGCG 2 cut(s) 138, 1053
BspHI TCATGA 1 cut(s) 787
BspLI GGNNCC 5 cut(s) 235, 370, 557, 852, 1049
BspMI ACCTGC 2 cut(s) 719, 1283
BspPI GGATC 4 cut(s) 295, 388, 544, 965
BspT107I GGYRCC 1 cut(s) 233
BsrDI GCAATG 1 cut(s) 1129
BsrI ACTGG 3 cut(s) 74, 467, 902
BssECI CCNNGG 2 cut(s) 23, 1051
BssMI GATC 4 cut(s) 287, 380, 536, 970
BssNI GRCGYC 2 cut(s) 234, 685
BssSI CACGAG 1 cut(s) 1305
BssT1I CCWWGG 1 cut(s) 23
Bst2BI CACGAG 1 cut(s) 1305
Bst2UI CCWGG 2 cut(s) 261, 830
Bst4CI ACNGT 5 cut(s) 54, 97, 488, 709, 1297
BstACI GRCGYC 2 cut(s) 234, 685
BstC8I GCNNGC 4 cut(s) 38, 508, 560, 1028
BstDEI CTNAG 4 cut(s) 450, 502, 858, 1149
BstDSI CCRYGG 1 cut(s) 1051
BstENI CCTNNNNNAGG 1 cut(s) 803
BstF5I GGATG 5 cut(s) 172, 497, 589, 641, 706
BstFNI CGCG 2 cut(s) 138, 1053
BstH2I RGCGCY 1 cut(s) 237
BstHHI GCGC 4 cut(s) 138, 236, 259, 497
BstKTI GATC 4 cut(s) 290, 383, 539, 973
BstMAI GTCTC 3 cut(s) 43, 869, 1145
BstMBI GATC 4 cut(s) 287, 380, 536, 970
BstMWI GCNNNNNNNGC 5 cut(s) 80, 233, 503, 620, 1023
BstNI CCWGG 2 cut(s) 261, 830
BstSCI CCNGG 2 cut(s) 259, 828
BstUI CGCG 2 cut(s) 138, 1053
BstV1I GCAGC 3 cut(s) 45, 92, 294
BstX2I RGATCY 3 cut(s) 287, 380, 970
BstXI CCANNNNNNTGG 1 cut(s) 394
BstYI RGATCY 3 cut(s) 287, 380, 970
BsuRI GGCC 4 cut(s) 59, 510, 558, 623
BtgI CCRYGG 1 cut(s) 1051
BtgZI GCGATG 1 cut(s) 153
BtrI CACGTC 1 cut(s) 1140
BtsCI GGATG 5 cut(s) 172, 497, 589, 641, 706
BtsIMutI CAGTG 3 cut(s) 909, 1070, 1293
BveI ACCTGC 2 cut(s) 719, 1283
Cac8I GCNNGC 4 cut(s) 38, 508, 560, 1028
CaiI CAGNNNCTG 1 cut(s) 1205
CciI TCATGA 1 cut(s) 787
CfoI GCGC 4 cut(s) 138, 236, 259, 497
Cfr13I GGNCC 6 cut(s) 57, 369, 397, 556, 622, 768
Cfr42I CCGCGG 1 cut(s) 1054
CseI GACGC 1 cut(s) 693
Csp6I GTAC 2 cut(s) 484, 1219
CviAII CATG 8 cut(s) 357, 394, 772, 788, 977, 1119, 1155, 1263
CviQI GTAC 2 cut(s) 484, 1219
DdeI CTNAG 4 cut(s) 450, 502, 858, 1149
DinI GGCGCC 1 cut(s) 235
DpnI GATC 4 cut(s) 289, 382, 538, 972
DpnII GATC 4 cut(s) 287, 380, 536, 970
EaeI YGGCCR 1 cut(s) 508
Eco130I CCWWGG 1 cut(s) 23
Eco32I GATATC 1 cut(s) 157
Eco47I GGWCC 3 cut(s) 369, 397, 768
Eco57I CTGAAG 5 cut(s) 548, 788, 894, 981, 1085
EcoNI CCTNNNNNAGG 1 cut(s) 803
EcoO109I RGGNCCY 2 cut(s) 369, 622
EcoRI GAATTC 1 cut(s) 1079
EcoRII CCWGG 2 cut(s) 259, 828
EcoRV GATATC 1 cut(s) 157
EcoT14I CCWWGG 1 cut(s) 23
EgeI GGCGCC 1 cut(s) 235
EheI GGCGCC 1 cut(s) 235
ErhI CCWWGG 1 cut(s) 23
Esp3I CGTCTC 1 cut(s) 43
FaeI CATG 8 cut(s) 360, 397, 775, 791, 980, 1122, 1158, 1266
FalI AAGNNNNNCTT 2 cut(s) 851, 883
FaqI GGGAC 1 cut(s) 382
FatI CATG 8 cut(s) 356, 393, 771, 787, 976, 1118, 1154, 1262
FauI CCCGC 1 cut(s) 170
FauNDI CATATG 1 cut(s) 1284
Fnu4HI GCNGC 3 cut(s) 34, 81, 308
FokI GGATG 5 cut(s) 159, 484, 596, 648, 713
Fsp4HI GCNGC 3 cut(s) 34, 81, 308
FspBI CTAG 4 cut(s) 24, 228, 911, 1023
GlaI GCGC 4 cut(s) 137, 235, 258, 496
GluI GCNGC 3 cut(s) 34, 81, 308
GsaI CCCAGC 1 cut(s) 923
GsuI CTGGAG 1 cut(s) 312
HaeII RGCGCY 1 cut(s) 237
HaeIII GGCC 4 cut(s) 59, 510, 558, 623
HapII CCGG 1 cut(s) 481
HgaI GACGC 1 cut(s) 693
HhaI GCGC 4 cut(s) 138, 236, 259, 497
Hin1I GRCGYC 2 cut(s) 234, 685
Hin1II CATG 8 cut(s) 360, 397, 775, 791, 980, 1122, 1158, 1266
Hin6I GCGC 4 cut(s) 136, 234, 257, 495
HinP1I GCGC 4 cut(s) 136, 234, 257, 495
HincII GTYRAC 1 cut(s) 916
HindII GTYRAC 1 cut(s) 916
HinfI GANTC 4 cut(s) 149, 347, 658, 1202
HpaII CCGG 1 cut(s) 481
HphI GGTGA 3 cut(s) 151, 172, 1155
Hpy166II GTNNAC 1 cut(s) 916
Hpy188I TCNGA 8 cut(s) 89, 154, 190, 442, 768, 970, 1085, 1230
Hpy188III TCNNGA 8 cut(s) 291, 449, 633, 740, 788, 872, 952, 1305
Hpy8I GTNNAC 1 cut(s) 916
Hpy99I CGWCG 2 cut(s) 89, 687
HpyAV CCTTC 4 cut(s) 115, 635, 812, 1060
HpyCH4III ACNGT 5 cut(s) 54, 97, 488, 709, 1297
HpyCH4IV ACGT 1 cut(s) 1139
HpyCH4V TGCA 9 cut(s) 214, 336, 704, 728, 826, 996, 1017, 1199, 1266
HpyF10VI GCNNNNNNNGC 5 cut(s) 80, 233, 503, 620, 1023
HpyF3I CTNAG 4 cut(s) 450, 502, 858, 1149
HpySE526I ACGT 1 cut(s) 1139
Hsp92I GRCGYC 2 cut(s) 234, 685
Hsp92II CATG 8 cut(s) 360, 397, 775, 791, 980, 1122, 1158, 1266
HspAI GCGC 4 cut(s) 136, 234, 257, 495
KasI GGCGCC 1 cut(s) 233
KspI CCGCGG 1 cut(s) 1054
Kzo9I GATC 4 cut(s) 287, 380, 536, 970
LmnI GCTCC 1 cut(s) 293
Lsp1109I GCAGC 3 cut(s) 45, 92, 294
LweI GCATC 6 cut(s) 506, 626, 691, 715, 974, 1050
MaeI CTAG 4 cut(s) 24, 228, 911, 1023
MaeII ACGT 1 cut(s) 1139
MaeIII GTNAC 1 cut(s) 838
MalI GATC 4 cut(s) 289, 382, 538, 972
MboI GATC 4 cut(s) 287, 380, 536, 970
MboII GAAGA 3 cut(s) 375, 541, 887
MfeI CAATTG 4 cut(s) 301, 1012, 1194, 1213
MflI RGATCY 3 cut(s) 287, 380, 970
MlsI TGGCCA 1 cut(s) 510
MluNI TGGCCA 1 cut(s) 510
Mly113I GGCGCC 1 cut(s) 234
MlyI GAGTC 3 cut(s) 143, 652, 1211
MmeI TCCRAC 4 cut(s) 67, 150, 784, 1208
Mox20I TGGCCA 1 cut(s) 510
MroXI GAANNNNTTC 1 cut(s) 1079
MscI TGGCCA 1 cut(s) 510
MseI TTAA 5 cut(s) 203, 425, 459, 885, 894
MslI CAYNNNNRTG 3 cut(s) 672, 902, 1261
Msp20I TGGCCA 1 cut(s) 510
MspA1I CMGCKG 1 cut(s) 1053
MspI CCGG 1 cut(s) 481
MspR9I CCNGG 2 cut(s) 261, 830
MunI CAATTG 4 cut(s) 301, 1012, 1194, 1213
Mva1269I GAATGC 1 cut(s) 1159
MvaI CCWGG 2 cut(s) 261, 830
MvnI CGCG 2 cut(s) 138, 1053
MwoI GCNNNNNNNGC 5 cut(s) 80, 233, 503, 620, 1023
NarI GGCGCC 1 cut(s) 234
NdeI CATATG 1 cut(s) 1284
NdeII GATC 4 cut(s) 287, 380, 536, 970
NlaIII CATG 8 cut(s) 360, 397, 775, 791, 980, 1122, 1158, 1266
NlaIV GGNNCC 5 cut(s) 235, 370, 557, 852, 1049
OliI CACNNNNGTG 1 cut(s) 902
PagI TCATGA 1 cut(s) 787
PaqCI CACCTGC 1 cut(s) 1283
PcsI WCGNNNNNNNCGW 1 cut(s) 93
PctI GAATGC 1 cut(s) 1159
PdmI GAANNNNTTC 1 cut(s) 1079
PfeI GAWTC 1 cut(s) 347
PflMI CCANNNNNTGG 1 cut(s) 132
PkrI GCNGC 3 cut(s) 35, 82, 309
PleI GAGTC 3 cut(s) 143, 652, 1210
PluTI GGCGCC 1 cut(s) 237
PpsI GAGTC 3 cut(s) 143, 652, 1210
PpuMI RGGWCCY 1 cut(s) 369
PsiI TTATAA 1 cut(s) 1112
Psp5II RGGWCCY 1 cut(s) 369
Psp6I CCWGG 2 cut(s) 259, 828
PspFI CCCAGC 1 cut(s) 919
PspGI CCWGG 2 cut(s) 259, 828
PspN4I GGNNCC 5 cut(s) 235, 370, 557, 852, 1049
PspPI GGNCC 6 cut(s) 57, 369, 397, 556, 622, 768
PspPPI RGGWCCY 1 cut(s) 369
PstNI CAGNNNCTG 1 cut(s) 1205
PsuI RGATCY 3 cut(s) 287, 380, 970
RsaI GTAC 2 cut(s) 485, 1220
RsaNI GTAC 2 cut(s) 484, 1219
RseI CAYNNNNRTG 3 cut(s) 672, 902, 1261
SacII CCGCGG 1 cut(s) 1054
SaqAI TTAA 5 cut(s) 203, 425, 459, 885, 894
SatI GCNGC 3 cut(s) 34, 81, 308
Sau3AI GATC 4 cut(s) 287, 380, 536, 970
Sau96I GGNCC 6 cut(s) 57, 369, 397, 556, 622, 768
SchI GAGTC 3 cut(s) 143, 652, 1211
ScrFI CCNGG 2 cut(s) 261, 830
SfaNI GCATC 6 cut(s) 506, 626, 691, 715, 974, 1050
SfoI GGCGCC 1 cut(s) 235
Sfr303I CCGCGG 1 cut(s) 1054
SgrBI CCGCGG 1 cut(s) 1054
SinI GGWCC 3 cut(s) 369, 397, 768
SmiMI CAYNNNNRTG 3 cut(s) 672, 902, 1261
SsiI CCGC 5 cut(s) 5, 163, 183, 1051, 1053
SspDI GGCGCC 1 cut(s) 233
SspMI CTAG 4 cut(s) 24, 228, 911, 1023
StyD4I CCNGG 2 cut(s) 259, 828
StyI CCWWGG 1 cut(s) 23
TaaI ACNGT 5 cut(s) 54, 97, 488, 709, 1297
TaiI ACGT 1 cut(s) 1142
TaqI TCGA 4 cut(s) 198, 682, 1058, 1248
TfiI GAWTC 1 cut(s) 347
Tru1I TTAA 5 cut(s) 203, 425, 459, 885, 894
Tru9I TTAA 5 cut(s) 203, 425, 459, 885, 894
TscAI CASTG 3 cut(s) 909, 1077, 1300
TseI GCWGC 3 cut(s) 33, 80, 307
TspDTI ATGAA 8 cut(s) 156, 161, 345, 600, 1092, 1107, 1118, 1301
TspGWI ACGGA 1 cut(s) 713
TspRI CASTG 3 cut(s) 909, 1077, 1300
Van91I CCANNNNNTGG 1 cut(s) 132
VpaK11BI GGWCC 3 cut(s) 369, 397, 768
XagI CCTNNNNNAGG 1 cut(s) 803
XapI RAATTY 3 cut(s) 1079, 1114, 1288
XcmI CCANNNNNNNNNTGG 1 cut(s) 981
XmaJI CCTAGG 1 cut(s) 23
XmnI GAANNNNTTC 1 cut(s) 1079
XspI CTAG 4 cut(s) 24, 228, 911, 1023
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.