pycom04g11590

Hydrolyzes acetyl esters in homogalacturonan regions of pectin. In type I primary cell wall, galacturonic acid residues of pectin can be acetylated at the O-2 and O-3 positions. Decreasing the degree of acetylation of pectin gels in vitro alters their physical properties

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr4
OG0001587
Physical Location & Seq
Forward (+)
14462123 .. 14466571
4449 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom04g11590.8

Sequence Viewer

Length: 1317 bp
ATGGTGGGGTCAGTGGCGAATCCCAGGCTTCGCAGCCTGCTATGGTGGCGAAGATGGGCCAAAAAGGACTGGGCAATCGCCGCCGTCGGATTTACCGTTATCGCCTTCGCTCTCACTCTCCTCTCCAACTCTTGGCGCGACGAACCAGACTCCGAAATCTCCCGCCCGTTTCTTCCCAACGCCGCCGCCTTCGATTTCGTTGACTTGACCTTGCTGCGCAACGCCAAAGATAGAGGCGCCCTTTGTTTGGACGGGACTGCCCCTGGCTACCATTTCAGGAAGGGATTCGGATCCGGCGCCAACAATTGGCTGCTTCACATTGAGGGTGGAGGTTGGTGCAATTCGATTGAATCATGTTCTTGGCGCAAAGGTACTCTACTAGGGTCTTCTAATTACATGGATCGTCGAGTTCCCTTTTTTGGGATTTTGAGCTCTCATCCATCACAAAATCCTGAATTCTTTAACTGGAACAAGGTCAAAATCAGATACTGTGATGGCGCGTCCTTCGCTGGCCACCCGGAAAATGAGCCTAAGAATGGATTAGGACCTTTTTTTAGGGGCCAGCTCATCTGGGAAGAAGTTATGGATGAACTCTTGTCAATTGGCTTGTCAAAAGTGAAACAGGCCCTTCTTTCAGGATGCTCTGCTGGTGGGTTAGCGACTCTTATACATTGTGATGACTTTCGACATCTTCTGCCTAAGGATGCAACTGTCAAATGTCTTGCTGATGCAGGTTTTTTCCTTGATGAGGAAGATGTTCTCCAGCATCGGACGATGAGGTCTTTCTATAATGATGTTGCCATCCTTCAGGGTTTATCAAAAAGTTTGAACAAGGATTGTCTTTCAAGGATGGAACCAGCTAAGTGTCTCTTTCCTGAAGAAGTTATTAAACACATTAATACCCCAGTGTTTCTTGTCAACCCAGCTTATGATTTTTGGCAGGTACAACATATCTTGATACCTGAAGCAGCAGATCCACATGACTATTGGAGAAAGTGTAAACTGAACATTCACAATTGCATTCCCAGCCAGCTTAAAACACTACATGGTTTCCGTGATTCTATGCTGAAAGCACTGACTGAATTCCAAAAAAATACGGAAGGAGGAATGTTTATAAATTCATGTTATATTCATTGCCAGACGTGGATAACCGAGACATGGCATTCACCCACTTCTCCACGAATAAACAATAAGACAATTGCAGAGTCTGTGGGCGACTGGTACTTTGGTCGAAATGCAGTAAAGCAAATTGACTGTCCATTCCCATGCAATCCCACCTGCTATCATATGAATTTCACTACTTTCTCACGAGGTTGA

Protein Analysis

439

Amino Acids

49.62

Weight (kDa)

7.86

Isoelectric Point (pI)

51.68

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1115
AarI CACCTGC 1 cut(s) 1286
AasI GACNNNNNNGTC 1 cut(s) 778
Acc16I TGCGCA 1 cut(s) 218
Acc36I ACCTGC 3 cut(s) 722, 931, 1286
AccB1I GGYRCC 2 cut(s) 236, 296
AccB7I CCANNNNNTGG 2 cut(s) 132, 306
AccII CGCG 2 cut(s) 138, 500
AciI CCGC 4 cut(s) 81, 163, 183, 186
AclWI GGATC 4 cut(s) 285, 298, 408, 968
AcoI YGGCCR 1 cut(s) 511
AcsI RAATTY 4 cut(s) 455, 1082, 1117, 1291
AcuI CTGAAG 3 cut(s) 791, 897, 984
AcyI GRCGYC 2 cut(s) 237, 297
AfaI GTAC 3 cut(s) 373, 945, 1223
AfiI CCNNNNNNNGG 8 cut(s) 132, 247, 306, 419, 420, 536, 748, 1158
AgsI TTSAA 3 cut(s) 350, 829, 846
AjiI CACGTC 1 cut(s) 1143
AjnI CCWGG 2 cut(s) 23, 262
AluBI AGCT 5 cut(s) 432, 565, 860, 926, 1033
AluI AGCT 5 cut(s) 432, 565, 860, 926, 1033
Alw21I GWGCWC 1 cut(s) 434
Alw26I GTCTC 2 cut(s) 872, 1148
AlwI GGATC 4 cut(s) 285, 298, 408, 968
AlwNI CAGNNNCTG 2 cut(s) 489, 1208
AoxI GGCC 4 cut(s) 57, 511, 559, 624
ApeKI GCWGC 4 cut(s) 33, 214, 310, 968
ApoI RAATTY 4 cut(s) 455, 1082, 1117, 1291
ArsI GACNNNNNNTTYG 4 cut(s) 1208, 1240, 1240, 1272
AseI ATTAAT 1 cut(s) 897
Asp700I GAANNNNTTC 2 cut(s) 284, 756
AspLEI GCGC 6 cut(s) 138, 219, 239, 299, 366, 500
AspS9I GGNCC 4 cut(s) 57, 545, 559, 625
AsuC2I CCSGG 1 cut(s) 518
AsuHPI GGTGA 1 cut(s) 1158
AvaII GGWCC 1 cut(s) 545
AxyI CCTNAGG 1 cut(s) 699
BaeI ACNNNNGTAYC 2 cut(s) 1213, 1246
BalI TGGCCA 1 cut(s) 513
BamHI GGATCC 1 cut(s) 290
BanI GGYRCC 2 cut(s) 236, 296
BanII GRGCYC 1 cut(s) 434
BauI CACGAG 1 cut(s) 1308
BbsI GAAGAC 1 cut(s) 378
Bbv12I GWGCWC 1 cut(s) 434
BbvI GCAGC 4 cut(s) 45, 201, 297, 980
BccI CCATC 5 cut(s) 48, 448, 488, 809, 844
BceAI ACGGC 1 cut(s) 68
BciT130I CCWGG 2 cut(s) 25, 264
BcnI CCSGG 1 cut(s) 518
BcoDI GTCTC 2 cut(s) 872, 1148
BfaI CTAG 1 cut(s) 380
BfoI RGCGCY 2 cut(s) 240, 300
BfuAI ACCTGC 3 cut(s) 722, 931, 1286
BisI GCNGC 7 cut(s) 34, 81, 183, 186, 215, 311, 969
BlsI GCNGC 7 cut(s) 35, 82, 184, 187, 216, 312, 970
Bme1390I CCNGG 3 cut(s) 25, 264, 518
Bme18I GGWCC 1 cut(s) 545
BmgBI CACGTC 1 cut(s) 1143
BmgT120I GGNCC 4 cut(s) 57, 545, 559, 625
BmiI GGNNCC 5 cut(s) 238, 292, 298, 560, 855
BmrFI CCNGG 3 cut(s) 25, 264, 518
BmrI ACTGGG 2 cut(s) 79, 899
BmsI GCATC 4 cut(s) 629, 694, 718, 775
BmuI ACTGGG 2 cut(s) 79, 899
BpiI GAAGAC 1 cut(s) 378
BpmI CTGGAG 1 cut(s) 746
BpuMI CCSGG 1 cut(s) 518
BsaBI GATNNNNATC 1 cut(s) 289
BsaHI GRCGYC 2 cut(s) 237, 297
BsaJI CCNNGG 2 cut(s) 23, 262
BsaXI ACNNNNNCTCC 2 cut(s) 982, 1012
Bsc4I CCNNNNNNNGG 8 cut(s) 132, 247, 306, 419, 420, 536, 748, 1158
Bse1I ACTGG 4 cut(s) 74, 470, 905, 1223
Bse21I CCTNAGG 1 cut(s) 699
Bse3DI GCAATG 1 cut(s) 1132
Bse8I GATNNNNATC 1 cut(s) 289
BseBI CCWGG 2 cut(s) 25, 264
BseDI CCNNGG 2 cut(s) 23, 262
BseGI GGATG 6 cut(s) 436, 592, 644, 709, 801, 855
BseJI GATNNNNATC 1 cut(s) 289
BseLI CCNNNNNNNGG 8 cut(s) 132, 247, 306, 419, 420, 536, 748, 1158
BseMI GCAATG 1 cut(s) 1132
BseNI ACTGG 4 cut(s) 74, 470, 905, 1223
BseRI GAGGAG 1 cut(s) 110
BseXI GCAGC 4 cut(s) 45, 201, 297, 980
BseYI CCCAGC 2 cut(s) 922, 1025
Bsh1236I CGCG 2 cut(s) 138, 500
BshFI GGCC 4 cut(s) 59, 513, 561, 626
BshNI GGYRCC 2 cut(s) 236, 296
BsiHKAI GWGCWC 1 cut(s) 434
BsiSI CCGG 2 cut(s) 294, 518
BslFI GGGAC 1 cut(s) 268
BslI CCNNNNNNNGG 8 cut(s) 132, 247, 306, 419, 420, 536, 748, 1158
BsmAI GTCTC 2 cut(s) 872, 1148
BsmFI GGGAC 1 cut(s) 268
BsmI GAATGC 2 cut(s) 1020, 1162
BsnI GGCC 4 cut(s) 59, 513, 561, 626
Bsp1286I GDGCHC 1 cut(s) 434
Bsp143I GATC 3 cut(s) 290, 400, 973
BspACI CCGC 4 cut(s) 81, 163, 183, 186
BspANI GGCC 4 cut(s) 59, 513, 561, 626
BspFNI CGCG 2 cut(s) 138, 500
BspLI GGNNCC 5 cut(s) 238, 292, 298, 560, 855
BspMI ACCTGC 3 cut(s) 722, 931, 1286
BspPI GGATC 4 cut(s) 285, 298, 408, 968
BspT107I GGYRCC 2 cut(s) 236, 296
BsrDI GCAATG 1 cut(s) 1132
BsrI ACTGG 4 cut(s) 74, 470, 905, 1223
BssECI CCNNGG 2 cut(s) 23, 262
BssMI GATC 3 cut(s) 290, 400, 973
BssNI GRCGYC 2 cut(s) 237, 297
BssSI CACGAG 1 cut(s) 1308
Bst2BI CACGAG 1 cut(s) 1308
Bst2UI CCWGG 2 cut(s) 25, 264
Bst4CI ACNGT 4 cut(s) 97, 491, 712, 1256
BstACI GRCGYC 2 cut(s) 237, 297
BstC8I GCNNGC 4 cut(s) 38, 511, 563, 1031
BstDEI CTNAG 3 cut(s) 531, 699, 861
BstENI CCTNNNNNAGG 1 cut(s) 746
BstF5I GGATG 6 cut(s) 436, 592, 644, 709, 801, 855
BstFNI CGCG 2 cut(s) 138, 500
BstH2I RGCGCY 2 cut(s) 240, 300
BstHHI GCGC 6 cut(s) 138, 219, 239, 299, 366, 500
BstKTI GATC 3 cut(s) 293, 403, 976
BstMAI GTCTC 2 cut(s) 872, 1148
BstMBI GATC 3 cut(s) 290, 400, 973
BstMWI GCNNNNNNNGC 4 cut(s) 46, 80, 506, 1026
BstNI CCWGG 2 cut(s) 25, 264
BstSCI CCNGG 3 cut(s) 23, 262, 516
BstUI CGCG 2 cut(s) 138, 500
BstV1I GCAGC 4 cut(s) 45, 201, 297, 980
BstV2I GAAGAC 1 cut(s) 378
BstX2I RGATCY 2 cut(s) 290, 973
BstYI RGATCY 2 cut(s) 290, 973
Bsu36I CCTNAGG 1 cut(s) 699
BsuRI GGCC 4 cut(s) 59, 513, 561, 626
BtrI CACGTC 1 cut(s) 1143
BtsCI GGATG 6 cut(s) 436, 592, 644, 709, 801, 855
BtsIMutI CAGTG 3 cut(s) 18, 912, 1073
BveI ACCTGC 3 cut(s) 722, 931, 1286
Cac8I GCNNGC 4 cut(s) 38, 511, 563, 1031
CaiI CAGNNNCTG 2 cut(s) 489, 1208
CfoI GCGC 6 cut(s) 138, 219, 239, 299, 366, 500
Cfr13I GGNCC 4 cut(s) 57, 545, 559, 625
CseI GACGC 1 cut(s) 489
Csp6I GTAC 3 cut(s) 372, 944, 1222
CviAII CATG 7 cut(s) 354, 397, 980, 1046, 1122, 1158, 1266
CviQI GTAC 3 cut(s) 372, 944, 1222
DdeI CTNAG 3 cut(s) 531, 699, 861
DinI GGCGCC 2 cut(s) 238, 298
DpnI GATC 3 cut(s) 292, 402, 975
DpnII GATC 3 cut(s) 290, 400, 973
DrdI GACNNNNNNGTC 1 cut(s) 778
DseDI GACNNNNNNGTC 1 cut(s) 778
EaeI YGGCCR 1 cut(s) 511
Ecl136II GAGCTC 1 cut(s) 432
Eco24I GRGCYC 1 cut(s) 434
Eco47I GGWCC 1 cut(s) 545
Eco53kI GAGCTC 1 cut(s) 432
Eco57I CTGAAG 3 cut(s) 791, 897, 984
Eco81I CCTNAGG 1 cut(s) 699
EcoICRI GAGCTC 1 cut(s) 432
EcoNI CCTNNNNNAGG 1 cut(s) 746
EcoO109I RGGNCCY 2 cut(s) 545, 625
EcoRI GAATTC 2 cut(s) 455, 1082
EcoRII CCWGG 2 cut(s) 23, 262
EcoT38I GRGCYC 1 cut(s) 434
EgeI GGCGCC 2 cut(s) 238, 298
EheI GGCGCC 2 cut(s) 238, 298
FaeI CATG 7 cut(s) 357, 400, 983, 1049, 1125, 1161, 1269
FalI AAGNNNNNCTT 2 cut(s) 854, 886
FaqI GGGAC 1 cut(s) 268
FatI CATG 7 cut(s) 353, 396, 979, 1045, 1121, 1157, 1265
FauI CCCGC 1 cut(s) 170
FauNDI CATATG 1 cut(s) 1287
Fnu4HI GCNGC 7 cut(s) 34, 81, 183, 186, 215, 311, 969
FokI GGATG 6 cut(s) 423, 599, 651, 716, 788, 862
FriOI GRGCYC 1 cut(s) 434
Fsp4HI GCNGC 7 cut(s) 34, 81, 183, 186, 215, 311, 969
FspBI CTAG 1 cut(s) 380
FspI TGCGCA 1 cut(s) 218
GlaI GCGC 6 cut(s) 137, 218, 238, 298, 365, 499
GluI GCNGC 7 cut(s) 34, 81, 183, 186, 215, 311, 969
GsaI CCCAGC 2 cut(s) 926, 1029
GsuI CTGGAG 1 cut(s) 746
HaeII RGCGCY 2 cut(s) 240, 300
HaeIII GGCC 4 cut(s) 59, 513, 561, 626
HapII CCGG 2 cut(s) 294, 518
HgaI GACGC 1 cut(s) 489
HhaI GCGC 6 cut(s) 138, 219, 239, 299, 366, 500
Hin1I GRCGYC 2 cut(s) 237, 297
Hin1II CATG 7 cut(s) 357, 400, 983, 1049, 1125, 1161, 1269
Hin6I GCGC 6 cut(s) 136, 217, 237, 297, 364, 498
HinP1I GCGC 6 cut(s) 136, 217, 237, 297, 364, 498
HincII GTYRAC 2 cut(s) 202, 919
HindII GTYRAC 2 cut(s) 202, 919
HinfI GANTC 7 cut(s) 19, 149, 285, 350, 661, 1058, 1205
HpaII CCGG 2 cut(s) 294, 518
HphI GGTGA 1 cut(s) 1158
Hpy166II GTNNAC 3 cut(s) 202, 919, 1001
Hpy188I TCNGA 5 cut(s) 89, 154, 290, 485, 771
Hpy188III TCNNGA 6 cut(s) 277, 452, 636, 875, 955, 1308
Hpy8I GTNNAC 3 cut(s) 202, 919, 1001
Hpy99I CGWCG 3 cut(s) 89, 143, 408
HpyAV CCTTC 7 cut(s) 115, 199, 274, 514, 638, 815, 1094
HpyCH4III ACNGT 4 cut(s) 97, 491, 712, 1256
HpyCH4IV ACGT 1 cut(s) 1142
HpyCH4V TGCA 7 cut(s) 339, 707, 731, 1020, 1202, 1238, 1269
HpyF10VI GCNNNNNNNGC 4 cut(s) 46, 80, 506, 1026
HpyF3I CTNAG 3 cut(s) 531, 699, 861
HpySE526I ACGT 1 cut(s) 1142
Hsp92I GRCGYC 2 cut(s) 237, 297
Hsp92II CATG 7 cut(s) 357, 400, 983, 1049, 1125, 1161, 1269
HspAI GCGC 6 cut(s) 136, 217, 237, 297, 364, 498
KasI GGCGCC 2 cut(s) 236, 296
Kzo9I GATC 3 cut(s) 290, 400, 973
Lsp1109I GCAGC 4 cut(s) 45, 201, 297, 980
LweI GCATC 4 cut(s) 629, 694, 718, 775
MaeI CTAG 1 cut(s) 380
MaeII ACGT 1 cut(s) 1142
MalI GATC 3 cut(s) 292, 402, 975
MboI GATC 3 cut(s) 290, 400, 973
MboII GAAGA 7 cut(s) 63, 164, 378, 587, 683, 764, 890
MfeI CAATTG 4 cut(s) 304, 600, 1015, 1197
MflI RGATCY 2 cut(s) 290, 973
MhlI GDGCHC 1 cut(s) 434
MlsI TGGCCA 1 cut(s) 513
MluNI TGGCCA 1 cut(s) 513
Mly113I GGCGCC 2 cut(s) 237, 297
MlyI GAGTC 3 cut(s) 143, 655, 1214
MmeI TCCRAC 2 cut(s) 67, 150
MnlI CCTC 8 cut(s) 131, 227, 316, 323, 742, 771, 1097, 1304
Mox20I TGGCCA 1 cut(s) 513
MroXI GAANNNNTTC 2 cut(s) 284, 756
MscI TGGCCA 1 cut(s) 513
MseI TTAA 4 cut(s) 462, 888, 897, 1035
MslI CAYNNNNRTG 2 cut(s) 675, 1264
Msp20I TGGCCA 1 cut(s) 513
MspI CCGG 2 cut(s) 294, 518
MspR9I CCNGG 3 cut(s) 25, 264, 518
MunI CAATTG 4 cut(s) 304, 600, 1015, 1197
Mva1269I GAATGC 2 cut(s) 1020, 1162
MvaI CCWGG 2 cut(s) 25, 264
MvnI CGCG 2 cut(s) 138, 500
MwoI GCNNNNNNNGC 4 cut(s) 46, 80, 506, 1026
NarI GGCGCC 2 cut(s) 237, 297
NciI CCSGG 1 cut(s) 518
NdeI CATATG 1 cut(s) 1287
NdeII GATC 3 cut(s) 290, 400, 973
NlaIII CATG 7 cut(s) 357, 400, 983, 1049, 1125, 1161, 1269
NlaIV GGNNCC 5 cut(s) 238, 292, 298, 560, 855
NsbI TGCGCA 1 cut(s) 218
PaqCI CACCTGC 1 cut(s) 1286
PcsI WCGNNNNNNNCGW 1 cut(s) 93
PctI GAATGC 2 cut(s) 1020, 1162
PdmI GAANNNNTTC 2 cut(s) 284, 756
PfeI GAWTC 4 cut(s) 19, 285, 350, 1058
PflMI CCANNNNNTGG 2 cut(s) 132, 306
PkrI GCNGC 7 cut(s) 35, 82, 184, 187, 216, 312, 970
PleI GAGTC 3 cut(s) 143, 655, 1213
PluTI GGCGCC 2 cut(s) 240, 300
PpsI GAGTC 3 cut(s) 143, 655, 1213
PpuMI RGGWCCY 1 cut(s) 545
PshBI ATTAAT 1 cut(s) 897
PsiI TTATAA 1 cut(s) 1115
Psp124BI GAGCTC 1 cut(s) 434
Psp5II RGGWCCY 1 cut(s) 545
Psp6I CCWGG 2 cut(s) 23, 262
PspFI CCCAGC 2 cut(s) 922, 1025
PspGI CCWGG 2 cut(s) 23, 262
PspN4I GGNNCC 5 cut(s) 238, 292, 298, 560, 855
PspPI GGNCC 4 cut(s) 57, 545, 559, 625
PspPPI RGGWCCY 1 cut(s) 545
PstNI CAGNNNCTG 2 cut(s) 489, 1208
PsuI RGATCY 2 cut(s) 290, 973
RsaI GTAC 3 cut(s) 373, 945, 1223
RsaNI GTAC 3 cut(s) 372, 944, 1222
RseI CAYNNNNRTG 2 cut(s) 675, 1264
SacI GAGCTC 1 cut(s) 434
SaqAI TTAA 4 cut(s) 462, 888, 897, 1035
SatI GCNGC 7 cut(s) 34, 81, 183, 186, 215, 311, 969
Sau3AI GATC 3 cut(s) 290, 400, 973
Sau96I GGNCC 4 cut(s) 57, 545, 559, 625
SchI GAGTC 3 cut(s) 143, 655, 1214
ScrFI CCNGG 3 cut(s) 25, 264, 518
SduI GDGCHC 1 cut(s) 434
SfaNI GCATC 4 cut(s) 629, 694, 718, 775
SfoI GGCGCC 2 cut(s) 238, 298
SinI GGWCC 1 cut(s) 545
SmiMI CAYNNNNRTG 2 cut(s) 675, 1264
SsiI CCGC 4 cut(s) 81, 163, 183, 186
SspDI GGCGCC 2 cut(s) 236, 296
SspMI CTAG 1 cut(s) 380
SstI GAGCTC 1 cut(s) 434
StyD4I CCNGG 3 cut(s) 23, 262, 516
TaaI ACNGT 4 cut(s) 97, 491, 712, 1256
TaiI ACGT 1 cut(s) 1145
TaqI TCGA 5 cut(s) 192, 344, 406, 685, 1231
TauI GCSGC 3 cut(s) 83, 185, 188
TfiI GAWTC 4 cut(s) 19, 285, 350, 1058
Tru1I TTAA 4 cut(s) 462, 888, 897, 1035
Tru9I TTAA 4 cut(s) 462, 888, 897, 1035
TscAI CASTG 3 cut(s) 18, 912, 1080
TseI GCWGC 4 cut(s) 33, 214, 310, 968
TspDTI ATGAA 4 cut(s) 603, 1110, 1121, 1304
TspGWI ACGGA 2 cut(s) 1043, 1112
TspRI CASTG 3 cut(s) 18, 912, 1080
Van91I CCANNNNNTGG 2 cut(s) 132, 306
VpaK11BI GGWCC 1 cut(s) 545
VspI ATTAAT 1 cut(s) 897
XagI CCTNNNNNAGG 1 cut(s) 746
XapI RAATTY 4 cut(s) 455, 1082, 1117, 1291
XcmI CCANNNNNNNNNTGG 1 cut(s) 984
XmnI GAANNNNTTC 2 cut(s) 284, 756
XspI CTAG 1 cut(s) 380
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.