pycom04g11500

Hydrolyzes acetyl esters in homogalacturonan regions of pectin. In type I primary cell wall, galacturonic acid residues of pectin can be acetylated at the O-2 and O-3 positions. Decreasing the degree of acetylation of pectin gels in vitro alters their physical properties

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr4
OG0001587
Physical Location & Seq
Forward (+)
14344847 .. 14347111
2265 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom04g11500.8

Sequence Viewer

Length: 921 bp
ATGGAAAATTGGCAGTTAATTTTTCAGTGTCCGTTTGACTGCGTGAGCAGAGCACCGGAAGCAGTCAGTCAATTGATGGTGGGGCCAATGGCGAATCCCAGGCTTCGCAGCCTTCTATGGTGGCGAAGGTGGGCCAAAAAGGACTGGGGAATCGCTGCCATCGGATGTACCGTTATCGCCTTCGCTCTCACTCTCCTCTCCAACTCTTGGCGCGACGAACCAGACTCCGAAATCTCCCGCCCGTTTCTTCCCAACGCCGCCGCCTTCGATTTCGTCGACTTGACCTTGCTGCGCAACGCCAAAGATAGAGGCGCCTTTTGTTTGGACGGGACTGCCCCTGGCTACCATTTCAGGAAGGGATTCGGATCCGGCGCCAACAATTGGCTGCTTCACATTGTGGGTGGAGGTTGGTGCAATTCGATTGAATCGTGTTCTTGGCGCAAAGGTACTCTACTAGGGTCTTCTAATTACATGGATTGTCGAGTTCCCTTTTCTGGGATGTTGAGCTCTCATCCATCACAAAATCCTGAATTCTTTAACTGGAACAAAGTCAAAATCCGATACTGTGATGGCGGATCCTTGGCTGGCCACCCGGAAAATGAGTCTAAGATTGGATCAGGACCTTTTTTTAGGGGCCAGCTCATCTGGGAAGCAGTTATGGATGAACTCTTGTCAATTGGCTTGTCAAAAGTGAAAGAGGCACTTCTTTCAGGATGCTCTGCCGGTGGGTTAGCGACTCTTATACATTGTGATGACTTTCGACGTCTTCTGCCAAAGGATGCAACTGTCAAATGTCTTGCTGATGCAGGTTTTTTCCTTGATGAAGAAGATGTTCTCCAGCATCGGACGATGAGGTCTTTCTATAATGACGTTGCCATCCTTGAGGATAGCTTTGATCTTTACTATTACTCAAGGTTCTAA

Protein Analysis

307

Amino Acids

34.32

Weight (kDa)

6.09

Isoelectric Point (pI)

49.03

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 853
AatII GACGTC 1 cut(s) 766
Acc16I TGCGCA 1 cut(s) 293
Acc36I ACCTGC 1 cut(s) 797
AccB1I GGYRCC 2 cut(s) 311, 371
AccB7I CCANNNNNTGG 2 cut(s) 207, 381
AccI GTMKAC 1 cut(s) 276
AccII CGCG 1 cut(s) 213
AciI CCGC 4 cut(s) 238, 258, 261, 573
AclWI GGATC 5 cut(s) 360, 373, 570, 583, 622
AcoI YGGCCR 1 cut(s) 586
AcsI RAATTY 1 cut(s) 530
AcyI GRCGYC 3 cut(s) 312, 372, 763
AdeI CACNNNGTG 1 cut(s) 397
AfaI GTAC 2 cut(s) 169, 448
AfiI CCNNNNNNNGG 4 cut(s) 207, 381, 494, 495
AgsI TTSAA 1 cut(s) 425
AjnI CCWGG 2 cut(s) 98, 337
AluBI AGCT 3 cut(s) 507, 640, 891
AluI AGCT 3 cut(s) 507, 640, 891
Alw21I GWGCWC 2 cut(s) 55, 509
AlwI GGATC 5 cut(s) 360, 373, 570, 583, 622
AoxI GGCC 4 cut(s) 83, 132, 586, 634
ApeKI GCWGC 4 cut(s) 108, 155, 289, 385
ApoI RAATTY 1 cut(s) 530
Asp700I GAANNNNTTC 2 cut(s) 359, 831
AspLEI GCGC 5 cut(s) 213, 294, 314, 374, 441
AspS9I GGNCC 4 cut(s) 83, 132, 620, 634
AsuC2I CCSGG 1 cut(s) 593
AvaII GGWCC 1 cut(s) 620
BalI TGGCCA 1 cut(s) 588
BamHI GGATCC 2 cut(s) 365, 575
BanI GGYRCC 2 cut(s) 311, 371
BanII GRGCYC 1 cut(s) 509
BbsI GAAGAC 2 cut(s) 453, 758
Bbv12I GWGCWC 2 cut(s) 55, 509
BbvI GCAGC 4 cut(s) 120, 142, 276, 372
BccI CCATC 5 cut(s) 70, 167, 523, 563, 884
BciT130I CCWGG 2 cut(s) 100, 339
BcnI CCSGG 1 cut(s) 593
BfaI CTAG 1 cut(s) 455
BfoI RGCGCY 2 cut(s) 315, 375
BfuAI ACCTGC 1 cut(s) 797
BisI GCNGC 6 cut(s) 109, 156, 258, 261, 290, 386
BlsI GCNGC 6 cut(s) 110, 157, 259, 262, 291, 387
Bme1390I CCNGG 3 cut(s) 100, 339, 593
Bme18I GGWCC 1 cut(s) 620
BmgT120I GGNCC 4 cut(s) 83, 132, 620, 634
BmiI GGNNCC 6 cut(s) 84, 313, 367, 373, 577, 635
BmrFI CCNGG 3 cut(s) 100, 339, 593
BmrI ACTGGG 1 cut(s) 154
BmsI GCATC 4 cut(s) 704, 769, 793, 850
BmuI ACTGGG 1 cut(s) 154
BpiI GAAGAC 2 cut(s) 453, 758
BpmI CTGGAG 1 cut(s) 821
BpuEI CTTGAG 2 cut(s) 895, 902
BpuMI CCSGG 1 cut(s) 593
BsaBI GATNNNNATC 1 cut(s) 364
BsaHI GRCGYC 3 cut(s) 312, 372, 763
BsaJI CCNNGG 3 cut(s) 98, 337, 579
BsaWI WCCGGW 1 cut(s) 55
Bsc4I CCNNNNNNNGG 4 cut(s) 207, 381, 494, 495
Bse118I RCCGGY 1 cut(s) 722
Bse1I ACTGG 2 cut(s) 149, 545
Bse8I GATNNNNATC 1 cut(s) 364
BseBI CCWGG 2 cut(s) 100, 339
BseDI CCNNGG 3 cut(s) 98, 337, 579
BseGI GGATG 7 cut(s) 170, 504, 511, 667, 719, 784, 876
BseJI GATNNNNATC 1 cut(s) 364
BseLI CCNNNNNNNGG 4 cut(s) 207, 381, 494, 495
BseNI ACTGG 2 cut(s) 149, 545
BseRI GAGGAG 1 cut(s) 185
BseXI GCAGC 4 cut(s) 120, 142, 276, 372
Bsh1236I CGCG 1 cut(s) 213
BshFI GGCC 4 cut(s) 85, 134, 588, 636
BshNI GGYRCC 2 cut(s) 311, 371
BsiHKAI GWGCWC 2 cut(s) 55, 509
BsiSI CCGG 4 cut(s) 56, 369, 593, 723
BslFI GGGAC 1 cut(s) 343
BslI CCNNNNNNNGG 4 cut(s) 207, 381, 494, 495
BsmFI GGGAC 1 cut(s) 343
BsnI GGCC 4 cut(s) 85, 134, 588, 636
Bsp1286I GDGCHC 2 cut(s) 55, 509
Bsp143I GATC 4 cut(s) 365, 575, 614, 895
BspACI CCGC 4 cut(s) 238, 258, 261, 573
BspANI GGCC 4 cut(s) 85, 134, 588, 636
BspFNI CGCG 1 cut(s) 213
BspLI GGNNCC 6 cut(s) 84, 313, 367, 373, 577, 635
BspMI ACCTGC 1 cut(s) 797
BspPI GGATC 5 cut(s) 360, 373, 570, 583, 622
BspT107I GGYRCC 2 cut(s) 311, 371
BsrFI RCCGGY 1 cut(s) 722
BsrI ACTGG 2 cut(s) 149, 545
BssAI RCCGGY 1 cut(s) 722
BssECI CCNNGG 3 cut(s) 98, 337, 579
BssMI GATC 4 cut(s) 365, 575, 614, 895
BssNI GRCGYC 3 cut(s) 312, 372, 763
BssT1I CCWWGG 1 cut(s) 579
Bst2UI CCWGG 2 cut(s) 100, 339
Bst4CI ACNGT 3 cut(s) 172, 566, 787
BstACI GRCGYC 3 cut(s) 312, 372, 763
BstC8I GCNNGC 2 cut(s) 586, 638
BstDEI CTNAG 1 cut(s) 606
BstF5I GGATG 7 cut(s) 170, 504, 511, 667, 719, 784, 876
BstFNI CGCG 1 cut(s) 213
BstH2I RGCGCY 2 cut(s) 315, 375
BstHHI GCGC 5 cut(s) 213, 294, 314, 374, 441
BstKTI GATC 4 cut(s) 368, 578, 617, 898
BstMBI GATC 4 cut(s) 365, 575, 614, 895
BstMWI GCNNNNNNNGC 1 cut(s) 59
BstNI CCWGG 2 cut(s) 100, 339
BstSCI CCNGG 3 cut(s) 98, 337, 591
BstUI CGCG 1 cut(s) 213
BstV1I GCAGC 4 cut(s) 120, 142, 276, 372
BstV2I GAAGAC 2 cut(s) 453, 758
BstX2I RGATCY 2 cut(s) 365, 575
BstYI RGATCY 2 cut(s) 365, 575
BsuRI GGCC 4 cut(s) 85, 134, 588, 636
BtsCI GGATG 7 cut(s) 170, 504, 511, 667, 719, 784, 876
BtsIMutI CAGTG 1 cut(s) 32
BveI ACCTGC 1 cut(s) 797
Cac8I GCNNGC 2 cut(s) 586, 638
CfoI GCGC 5 cut(s) 213, 294, 314, 374, 441
Cfr10I RCCGGY 1 cut(s) 722
Cfr13I GGNCC 4 cut(s) 83, 132, 620, 634
Csp6I GTAC 2 cut(s) 168, 447
CviAII CATG 1 cut(s) 472
CviQI GTAC 2 cut(s) 168, 447
DdeI CTNAG 1 cut(s) 606
DinI GGCGCC 2 cut(s) 313, 373
DpnI GATC 4 cut(s) 367, 577, 616, 897
DpnII GATC 4 cut(s) 365, 575, 614, 895
DraIII CACNNNGTG 1 cut(s) 397
DrdI GACNNNNNNGTC 1 cut(s) 853
DseDI GACNNNNNNGTC 1 cut(s) 853
EaeI YGGCCR 1 cut(s) 586
EciI GGCGGA 1 cut(s) 588
Ecl136II GAGCTC 1 cut(s) 507
Eco130I CCWWGG 1 cut(s) 579
Eco24I GRGCYC 1 cut(s) 509
Eco47I GGWCC 1 cut(s) 620
Eco53kI GAGCTC 1 cut(s) 507
EcoICRI GAGCTC 1 cut(s) 507
EcoO109I RGGNCCY 1 cut(s) 620
EcoRI GAATTC 1 cut(s) 530
EcoRII CCWGG 2 cut(s) 98, 337
EcoT14I CCWWGG 1 cut(s) 579
EcoT38I GRGCYC 1 cut(s) 509
EgeI GGCGCC 2 cut(s) 313, 373
EheI GGCGCC 2 cut(s) 313, 373
ErhI CCWWGG 1 cut(s) 579
FaeI CATG 1 cut(s) 475
FaiI YATR 5 cut(s) 118, 473, 659, 743, 864
FalI AAGNNNNNCTT 2 cut(s) 687, 719
FaqI GGGAC 1 cut(s) 343
FatI CATG 1 cut(s) 471
FauI CCCGC 1 cut(s) 245
FblI GTMKAC 1 cut(s) 276
Fnu4HI GCNGC 6 cut(s) 109, 156, 258, 261, 290, 386
FokI GGATG 7 cut(s) 177, 498, 511, 674, 726, 791, 863
FriOI GRGCYC 1 cut(s) 509
Fsp4HI GCNGC 6 cut(s) 109, 156, 258, 261, 290, 386
FspBI CTAG 1 cut(s) 455
FspI TGCGCA 1 cut(s) 293
GlaI GCGC 5 cut(s) 212, 293, 313, 373, 440
GluI GCNGC 6 cut(s) 109, 156, 258, 261, 290, 386
GsuI CTGGAG 1 cut(s) 821
HaeII RGCGCY 2 cut(s) 315, 375
HaeIII GGCC 4 cut(s) 85, 134, 588, 636
HapII CCGG 4 cut(s) 56, 369, 593, 723
HhaI GCGC 5 cut(s) 213, 294, 314, 374, 441
Hin1I GRCGYC 3 cut(s) 312, 372, 763
Hin1II CATG 1 cut(s) 475
Hin6I GCGC 5 cut(s) 211, 292, 312, 372, 439
HinP1I GCGC 5 cut(s) 211, 292, 312, 372, 439
HincII GTYRAC 1 cut(s) 277
HindII GTYRAC 1 cut(s) 277
HinfI GANTC 7 cut(s) 94, 150, 224, 360, 425, 602, 736
HpaII CCGG 4 cut(s) 56, 369, 593, 723
Hpy166II GTNNAC 1 cut(s) 277
Hpy188I TCNGA 5 cut(s) 164, 229, 365, 560, 846
Hpy188III TCNNGA 4 cut(s) 352, 527, 618, 711
Hpy8I GTNNAC 1 cut(s) 277
Hpy99I CGWCG 3 cut(s) 218, 278, 765
HpyAV CCTTC 5 cut(s) 120, 122, 190, 274, 349
HpyCH4III ACNGT 3 cut(s) 172, 566, 787
HpyCH4IV ACGT 2 cut(s) 763, 870
HpyCH4V TGCA 3 cut(s) 414, 782, 806
HpyF10VI GCNNNNNNNGC 1 cut(s) 59
HpyF3I CTNAG 1 cut(s) 606
HpySE526I ACGT 2 cut(s) 763, 870
Hsp92I GRCGYC 3 cut(s) 312, 372, 763
Hsp92II CATG 1 cut(s) 475
HspAI GCGC 5 cut(s) 211, 292, 312, 372, 439
KasI GGCGCC 2 cut(s) 311, 371
Kzo9I GATC 4 cut(s) 365, 575, 614, 895
Lsp1109I GCAGC 4 cut(s) 120, 142, 276, 372
LweI GCATC 4 cut(s) 704, 769, 793, 850
MaeI CTAG 1 cut(s) 455
MaeII ACGT 2 cut(s) 763, 870
MalI GATC 4 cut(s) 367, 577, 616, 897
MboI GATC 4 cut(s) 365, 575, 614, 895
MboII GAAGA 5 cut(s) 239, 453, 758, 836, 839
MfeI CAATTG 3 cut(s) 71, 379, 675
MflI RGATCY 2 cut(s) 365, 575
MhlI GDGCHC 2 cut(s) 55, 509
MlsI TGGCCA 1 cut(s) 588
MluCI AATT 8 cut(s) 7, 18, 71, 379, 415, 466, 530, 675
MluNI TGGCCA 1 cut(s) 588
Mly113I GGCGCC 2 cut(s) 312, 372
MlyI GAGTC 3 cut(s) 218, 611, 730
MmeI TCCRAC 1 cut(s) 225
MnlI CCTC 6 cut(s) 206, 302, 398, 691, 846, 877
Mox20I TGGCCA 1 cut(s) 588
MroXI GAANNNNTTC 2 cut(s) 359, 831
MscI TGGCCA 1 cut(s) 588
MseI TTAA 2 cut(s) 17, 537
MslI CAYNNNNRTG 1 cut(s) 750
Msp20I TGGCCA 1 cut(s) 588
MspI CCGG 4 cut(s) 56, 369, 593, 723
MspR9I CCNGG 3 cut(s) 100, 339, 593
MunI CAATTG 3 cut(s) 71, 379, 675
MvaI CCWGG 2 cut(s) 100, 339
MvnI CGCG 1 cut(s) 213
MwoI GCNNNNNNNGC 1 cut(s) 59
NarI GGCGCC 2 cut(s) 312, 372
NciI CCSGG 1 cut(s) 593
NdeII GATC 4 cut(s) 365, 575, 614, 895
NlaIII CATG 1 cut(s) 475
NlaIV GGNNCC 6 cut(s) 84, 313, 367, 373, 577, 635
NsbI TGCGCA 1 cut(s) 293
PcsI WCGNNNNNNNCGW 3 cut(s) 168, 273, 425
PdmI GAANNNNTTC 2 cut(s) 359, 831
PfeI GAWTC 4 cut(s) 94, 150, 360, 425
PflMI CCANNNNNTGG 2 cut(s) 207, 381
PkrI GCNGC 6 cut(s) 110, 157, 259, 262, 291, 387
PleI GAGTC 3 cut(s) 218, 610, 730
PluTI GGCGCC 2 cut(s) 315, 375
PpsI GAGTC 3 cut(s) 218, 610, 730
PpuMI RGGWCCY 1 cut(s) 620
Psp124BI GAGCTC 1 cut(s) 509
Psp5II RGGWCCY 1 cut(s) 620
Psp6I CCWGG 2 cut(s) 98, 337
PspGI CCWGG 2 cut(s) 98, 337
PspN4I GGNNCC 6 cut(s) 84, 313, 367, 373, 577, 635
PspPI GGNCC 4 cut(s) 83, 132, 620, 634
PspPPI RGGWCCY 1 cut(s) 620
PsuI RGATCY 2 cut(s) 365, 575
RsaI GTAC 2 cut(s) 169, 448
RsaNI GTAC 2 cut(s) 168, 447
RseI CAYNNNNRTG 1 cut(s) 750
SacI GAGCTC 1 cut(s) 509
SalI GTCGAC 1 cut(s) 275
SaqAI TTAA 2 cut(s) 17, 537
SatI GCNGC 6 cut(s) 109, 156, 258, 261, 290, 386
Sau3AI GATC 4 cut(s) 365, 575, 614, 895
Sau96I GGNCC 4 cut(s) 83, 132, 620, 634
SchI GAGTC 3 cut(s) 218, 611, 730
ScrFI CCNGG 3 cut(s) 100, 339, 593
SduI GDGCHC 2 cut(s) 55, 509
SfaNI GCATC 4 cut(s) 704, 769, 793, 850
SfoI GGCGCC 2 cut(s) 313, 373
SinI GGWCC 1 cut(s) 620
SmiMI CAYNNNNRTG 1 cut(s) 750
SmlI CTYRAG 2 cut(s) 881, 910
SmoI CTYRAG 2 cut(s) 881, 910
Sse9I AATT 8 cut(s) 7, 18, 71, 379, 415, 466, 530, 675
SsiI CCGC 4 cut(s) 238, 258, 261, 573
SspDI GGCGCC 2 cut(s) 311, 371
SspMI CTAG 1 cut(s) 455
SstI GAGCTC 1 cut(s) 509
StyD4I CCNGG 3 cut(s) 98, 337, 591
StyI CCWWGG 1 cut(s) 579
TaaI ACNGT 3 cut(s) 172, 566, 787
TaiI ACGT 2 cut(s) 766, 873
TaqI TCGA 5 cut(s) 267, 276, 419, 481, 760
TasI AATT 8 cut(s) 7, 18, 71, 379, 415, 466, 530, 675
TauI GCSGC 2 cut(s) 260, 263
TfiI GAWTC 4 cut(s) 94, 150, 360, 425
Tru1I TTAA 2 cut(s) 17, 537
Tru9I TTAA 2 cut(s) 17, 537
TscAI CASTG 1 cut(s) 32
TseI GCWGC 4 cut(s) 108, 155, 289, 385
TspDTI ATGAA 2 cut(s) 678, 837
TspGWI ACGGA 1 cut(s) 21
TspRI CASTG 1 cut(s) 32
Van91I CCANNNNNTGG 2 cut(s) 207, 381
VpaK11BI GGWCC 1 cut(s) 620
XapI RAATTY 1 cut(s) 530
XmiI GTMKAC 1 cut(s) 276
XmnI GAANNNNTTC 2 cut(s) 359, 831
XspI CTAG 1 cut(s) 455
ZraI GACGTC 1 cut(s) 764
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.