Rroxscaffold_6G00414210

Hydrolyzes acetyl esters in homogalacturonan regions of pectin. In type I primary cell wall, galacturonic acid residues of pectin can be acetylated at the O-2 and O-3 positions. Decreasing the degree of acetylation of pectin gels in vitro alters their physical properties

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000006
OG0001587
Physical Location & Seq
Forward (+)
36491004 .. 36495040
4037 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_6G00414210.1

Sequence Viewer

Length: 1314 bp
ATGGCGGGGTCAACGGCGAACCTCAGACTCCGCAGCCTTGTATGGTGGAGAAAGTGGGCCAGAAAGGACTGGGCGATCTCCACCCTCGCCTTTACCTTCATTGTCTTCCTTCTCACCCTCATCTCCAACTCTTGGCGCGACGAGCCTGACTCCGGCTTCACCAACCCCTTCGATCCAACCACCGCCGCCGATTTGGTCGACTTGACTCTGCTGCACGACGCCGAACGCAGAGGCGCCCTTTGCTTAGATGGGAGTGCTCCCGGGTACCATTTCCGAAAGGGGTTCGGATCCGGAGCCAAAAATTGGCTGCTTCACATTGAGGGTGGAGGTTGGTGCAATACAATAGAATCATGTTCTTTTCGGAAAAGGACTCCATTAGGGTCTTCCAAGTACATGGATCGTAGAGTTCCGTTTTCCGGGATTTTGAGCTCTCACCCGTCAGAAAATCCTGACTTCTTTAACTGGAACAAAGTCAAAATACGGTACTGTGATGGCGCGTCTTTTGCTGGCCACCCTGAAAATGAGCCCAAGAATGGATCAAAACTTTTCTTTAGGGGACAGCTTATCTGGGAAGCACTCATGGATGAACTCTTGTCAATTGGCTTGTCAAAGGCGAAACAGGCACTTCTTTCTGGATGCTCTGCTGGTGGGTTGGCAACTCTTATACACTGTGATGACTTTCGAGGTCTTTTACCTAGGGATTCAACTGTCAAATGTCTTGCTGATGCAGGTTTTTTTCTCGATGAGAAAGATGTTAACCGAAACCGGACCATGAGAACTTTTTATCGTGATGTTGTGGTTCTTCAGGATTTAGCAAAAAGTTTGCACAAGGATTGTGTTGCGAGAAAGGAACCAGCTAAGTGTCTCTTTCCCGAAGAAATTATTAAACACGTTAGCACTCCAGTGTTCCTTGTCAACCCAGCTTATGATTTTTGGCAGATACAACATATCTTGATACCTGAAGCATCAGATCCGCATGGCTACTGGAAAAAGTGCAAGCTCAACATCTATAATTGCAATCCTAGCCAGATTGAAACACTGCAAGGTTTCCGTCGTTCTATGCTAAATGCACTGAACGAATTCCAGAAAAATAAGGAGGGAGGAACTTTTATAAATTCATGTTTTATTCATTGCCAGACATGGTTTTCAGAGACATGGCAGTCACCCAGTTCTCCACGAATAAACAATAAGACAATTGCAGAGTCTGTGGGTGATTGGTACTTTAATCGAAATGTTGCAAAGCAAATTGACTGCTCATTTCCATGCAATCCCACCTGTTATCATATGAATTTCACTCAATTTTCACGAGGTTGA

Protein Analysis

437

Amino Acids

49.68

Weight (kDa)

8.7

Isoelectric Point (pI)

46.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PAE PF03283 63 - 426 2.2e-164 Pectinacetylesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1112
AasI GACNNNNNNGTC 1 cut(s) 1159
Acc36I ACCTGC 1 cut(s) 719
Acc65I GGTACC 1 cut(s) 264
AccB1I GGYRCC 2 cut(s) 233, 264
AccB7I CCANNNNNTGG 2 cut(s) 132, 303
AccI GTMKAC 1 cut(s) 198
AccII CGCG 2 cut(s) 138, 497
AccIII TCCGGA 1 cut(s) 290
AciI CCGC 5 cut(s) 5, 31, 183, 186, 974
AclWI GGATC 6 cut(s) 167, 282, 295, 405, 544, 965
AcoI YGGCCR 1 cut(s) 508
AcsI RAATTY 3 cut(s) 1079, 1114, 1288
AcuI CTGAAG 2 cut(s) 788, 981
AcyI GRCGYC 2 cut(s) 219, 234
AfaI GTAC 4 cut(s) 266, 392, 485, 1220
AfiI CCNNNNNNNGG 5 cut(s) 132, 152, 303, 416, 533
AflIII ACRYGT 1 cut(s) 889
AgsI TTSAA 2 cut(s) 705, 1034
AleI CACNNNNGTG 1 cut(s) 902
AluBI AGCT 5 cut(s) 429, 562, 857, 923, 1000
AluI AGCT 5 cut(s) 429, 562, 857, 923, 1000
Alw21I GWGCWC 2 cut(s) 259, 431
Alw26I GTCTC 2 cut(s) 869, 1145
AlwI GGATC 6 cut(s) 167, 282, 295, 405, 544, 965
AlwNI CAGNNNCTG 1 cut(s) 1205
Ama87I CYCGRG 1 cut(s) 260
Aor13HI TCCGGA 1 cut(s) 290
AoxI GGCC 2 cut(s) 57, 508
ApeKI GCWGC 3 cut(s) 33, 211, 307
ApoI RAATTY 3 cut(s) 1079, 1114, 1288
Asp700I GAANNNNTTC 1 cut(s) 1079
Asp718I GGTACC 1 cut(s) 264
AspA2I CCTAGG 1 cut(s) 695
AspLEI GCGC 3 cut(s) 138, 236, 497
AspS9I GGNCC 2 cut(s) 57, 768
AsuC2I CCSGG 3 cut(s) 261, 262, 418
AsuHPI GGTGA 5 cut(s) 106, 151, 425, 1155, 1223
AvaI CYCGRG 1 cut(s) 260
AvaII GGWCC 1 cut(s) 768
AvrII CCTAGG 1 cut(s) 695
BalI TGGCCA 1 cut(s) 510
BamHI GGATCC 1 cut(s) 287
BanI GGYRCC 2 cut(s) 233, 264
BanII GRGCYC 2 cut(s) 431, 528
BauI CACGAG 1 cut(s) 1305
BbsI GAAGAC 2 cut(s) 97, 375
Bbv12I GWGCWC 2 cut(s) 259, 431
BbvI GCAGC 3 cut(s) 45, 198, 294
BccI CCATC 2 cut(s) 242, 485
BceAI ACGGC 1 cut(s) 30
BcnI CCSGG 3 cut(s) 261, 262, 418
BcoDI GTCTC 2 cut(s) 869, 1145
BfaI CTAG 2 cut(s) 696, 1023
BfoI RGCGCY 1 cut(s) 237
BfuAI ACCTGC 1 cut(s) 719
BisI GCNGC 4 cut(s) 34, 186, 212, 308
BlnI CCTAGG 1 cut(s) 695
BlsI GCNGC 4 cut(s) 35, 187, 213, 309
Bme1390I CCNGG 3 cut(s) 261, 262, 418
Bme18I GGWCC 1 cut(s) 768
BmeT110I CYCGRG 1 cut(s) 260
BmgT120I GGNCC 2 cut(s) 57, 768
BmiI GGNNCC 5 cut(s) 235, 266, 289, 295, 852
BmrFI CCNGG 3 cut(s) 261, 262, 418
BmrI ACTGGG 2 cut(s) 79, 1161
BmsI GCATC 3 cut(s) 626, 715, 974
BmuI ACTGGG 2 cut(s) 79, 1161
BpiI GAAGAC 2 cut(s) 97, 375
BplI GAGNNNNNCTC 2 cut(s) 134, 166
BpmI CTGGAG 1 cut(s) 885
BpuMI CCSGG 3 cut(s) 261, 262, 418
BsaHI GRCGYC 2 cut(s) 219, 234
BsaJI CCNNGG 2 cut(s) 260, 695
BsaWI WCCGGW 2 cut(s) 290, 765
Bsc4I CCNNNNNNNGG 5 cut(s) 132, 152, 303, 416, 533
Bse1I ACTGG 5 cut(s) 74, 467, 902, 989, 1167
Bse3DI GCAATG 1 cut(s) 1129
BseAI TCCGGA 1 cut(s) 290
BseDI CCNNGG 2 cut(s) 260, 695
BseGI GGATG 2 cut(s) 589, 641
BseLI CCNNNNNNNGG 5 cut(s) 132, 152, 303, 416, 533
BseMI GCAATG 1 cut(s) 1129
BseMII CTCAG 1 cut(s) 37
BseNI ACTGG 5 cut(s) 74, 467, 902, 989, 1167
BseXI GCAGC 3 cut(s) 45, 198, 294
BseYI CCCAGC 1 cut(s) 919
BsgI GTGCAG 1 cut(s) 197
Bsh1236I CGCG 2 cut(s) 138, 497
BshFI GGCC 2 cut(s) 59, 510
BshNI GGYRCC 2 cut(s) 233, 264
BsiHKAI GWGCWC 2 cut(s) 259, 431
BsiHKCI CYCGRG 1 cut(s) 260
BsiSI CCGG 5 cut(s) 153, 261, 291, 417, 766
BslFI GGGAC 1 cut(s) 570
BslI CCNNNNNNNGG 5 cut(s) 132, 152, 303, 416, 533
BsmAI GTCTC 2 cut(s) 869, 1145
BsmFI GGGAC 1 cut(s) 570
BsnI GGCC 2 cut(s) 59, 510
BsoBI CYCGRG 1 cut(s) 260
Bsp1286I GDGCHC 3 cut(s) 259, 431, 528
Bsp13I TCCGGA 1 cut(s) 290
Bsp143I GATC 6 cut(s) 75, 172, 287, 397, 536, 970
BspACI CCGC 5 cut(s) 5, 31, 183, 186, 974
BspANI GGCC 2 cut(s) 59, 510
BspCNI CTCAG 1 cut(s) 36
BspEI TCCGGA 1 cut(s) 290
BspFNI CGCG 2 cut(s) 138, 497
BspLI GGNNCC 5 cut(s) 235, 266, 289, 295, 852
BspMI ACCTGC 1 cut(s) 719
BspPI GGATC 6 cut(s) 167, 282, 295, 405, 544, 965
BspT107I GGYRCC 2 cut(s) 233, 264
BsrDI GCAATG 1 cut(s) 1129
BsrI ACTGG 5 cut(s) 74, 467, 902, 989, 1167
BssECI CCNNGG 2 cut(s) 260, 695
BssMI GATC 6 cut(s) 75, 172, 287, 397, 536, 970
BssNI GRCGYC 2 cut(s) 219, 234
BssSI CACGAG 1 cut(s) 1305
BssT1I CCWWGG 1 cut(s) 695
Bst2BI CACGAG 1 cut(s) 1305
Bst4CI ACNGT 4 cut(s) 483, 488, 671, 709
BstACI GRCGYC 2 cut(s) 219, 234
BstC8I GCNNGC 2 cut(s) 508, 998
BstDEI CTNAG 3 cut(s) 23, 244, 858
BstF5I GGATG 2 cut(s) 589, 641
BstFNI CGCG 2 cut(s) 138, 497
BstH2I RGCGCY 1 cut(s) 237
BstHHI GCGC 3 cut(s) 138, 236, 497
BstKTI GATC 6 cut(s) 78, 175, 290, 400, 539, 973
BstMAI GTCTC 2 cut(s) 869, 1145
BstMBI GATC 6 cut(s) 75, 172, 287, 397, 536, 970
BstMWI GCNNNNNNNGC 5 cut(s) 142, 240, 503, 620, 1023
BstSCI CCNGG 3 cut(s) 259, 260, 416
BstUI CGCG 2 cut(s) 138, 497
BstV1I GCAGC 3 cut(s) 45, 198, 294
BstV2I GAAGAC 2 cut(s) 97, 375
BstX2I RGATCY 2 cut(s) 287, 970
BstXI CCANNNNNNTGG 1 cut(s) 394
BstYI RGATCY 2 cut(s) 287, 970
BsuRI GGCC 2 cut(s) 59, 510
BtsCI GGATG 2 cut(s) 589, 641
BtsI GCAGTG 1 cut(s) 1037
BtsIMutI CAGTG 4 cut(s) 667, 909, 1037, 1070
BveI ACCTGC 1 cut(s) 719
Cac8I GCNNGC 2 cut(s) 508, 998
CaiI CAGNNNCTG 1 cut(s) 1205
CfoI GCGC 3 cut(s) 138, 236, 497
Cfr13I GGNCC 2 cut(s) 57, 768
Cfr9I CCCGGG 1 cut(s) 260
CseI GACGC 2 cut(s) 227, 486
Csp6I GTAC 4 cut(s) 265, 391, 484, 1219
CviAII CATG 9 cut(s) 351, 394, 580, 772, 977, 1119, 1140, 1155, 1263
CviQI GTAC 4 cut(s) 265, 391, 484, 1219
DdeI CTNAG 3 cut(s) 23, 244, 858
DinI GGCGCC 1 cut(s) 235
DpnI GATC 6 cut(s) 77, 174, 289, 399, 538, 972
DpnII GATC 6 cut(s) 75, 172, 287, 397, 536, 970
DrdI GACNNNNNNGTC 1 cut(s) 1159
DseDI GACNNNNNNGTC 1 cut(s) 1159
EaeI YGGCCR 1 cut(s) 508
Ecl136II GAGCTC 1 cut(s) 429
Eco130I CCWWGG 1 cut(s) 695
Eco24I GRGCYC 2 cut(s) 431, 528
Eco47I GGWCC 1 cut(s) 768
Eco53kI GAGCTC 1 cut(s) 429
Eco57I CTGAAG 2 cut(s) 788, 981
Eco88I CYCGRG 1 cut(s) 260
EcoICRI GAGCTC 1 cut(s) 429
EcoRI GAATTC 1 cut(s) 1079
EcoT14I CCWWGG 1 cut(s) 695
EcoT38I GRGCYC 2 cut(s) 431, 528
EgeI GGCGCC 1 cut(s) 235
EheI GGCGCC 1 cut(s) 235
ErhI CCWWGG 1 cut(s) 695
FaeI CATG 9 cut(s) 354, 397, 583, 775, 980, 1122, 1143, 1158, 1266
FalI AAGNNNNNCTT 2 cut(s) 851, 883
FaqI GGGAC 1 cut(s) 570
FatI CATG 9 cut(s) 350, 393, 579, 771, 976, 1118, 1139, 1154, 1262
FauNDI CATATG 1 cut(s) 1284
FblI GTMKAC 1 cut(s) 198
Fnu4HI GCNGC 4 cut(s) 34, 186, 212, 308
FokI GGATG 2 cut(s) 596, 648
FriOI GRGCYC 2 cut(s) 431, 528
Fsp4HI GCNGC 4 cut(s) 34, 186, 212, 308
FspBI CTAG 2 cut(s) 696, 1023
GlaI GCGC 3 cut(s) 137, 235, 496
GluI GCNGC 4 cut(s) 34, 186, 212, 308
GsaI CCCAGC 1 cut(s) 923
GsuI CTGGAG 1 cut(s) 885
HaeII RGCGCY 1 cut(s) 237
HaeIII GGCC 2 cut(s) 59, 510
HapII CCGG 5 cut(s) 153, 261, 291, 417, 766
HgaI GACGC 2 cut(s) 227, 486
HhaI GCGC 3 cut(s) 138, 236, 497
Hin1I GRCGYC 2 cut(s) 219, 234
Hin1II CATG 9 cut(s) 354, 397, 583, 775, 980, 1122, 1143, 1158, 1266
Hin6I GCGC 3 cut(s) 136, 234, 495
HinP1I GCGC 3 cut(s) 136, 234, 495
HincII GTYRAC 4 cut(s) 12, 199, 757, 916
HindII GTYRAC 4 cut(s) 12, 199, 757, 916
HinfI GANTC 7 cut(s) 27, 149, 205, 347, 370, 701, 1202
HpaI GTTAAC 1 cut(s) 757
HpaII CCGG 5 cut(s) 153, 261, 291, 417, 766
HphI GGTGA 5 cut(s) 106, 151, 425, 1155, 1223
Hpy166II GTNNAC 4 cut(s) 12, 199, 757, 916
Hpy188I TCNGA 7 cut(s) 26, 275, 287, 363, 442, 970, 1150
Hpy8I GTNNAC 4 cut(s) 12, 199, 757, 916
Hpy99I CGWCG 3 cut(s) 143, 221, 1056
HpyAV CCTTC 3 cut(s) 106, 119, 178
HpyCH4III ACNGT 4 cut(s) 483, 488, 671, 709
HpyCH4IV ACGT 1 cut(s) 891
HpyF10VI GCNNNNNNNGC 5 cut(s) 142, 240, 503, 620, 1023
HpyF3I CTNAG 3 cut(s) 23, 244, 858
HpySE526I ACGT 1 cut(s) 891
Hsp92I GRCGYC 2 cut(s) 219, 234
Hsp92II CATG 9 cut(s) 354, 397, 583, 775, 980, 1122, 1143, 1158, 1266
HspAI GCGC 3 cut(s) 136, 234, 495
KasI GGCGCC 1 cut(s) 233
Kpn2I TCCGGA 1 cut(s) 290
KpnI GGTACC 1 cut(s) 268
KspAI GTTAAC 1 cut(s) 757
Kzo9I GATC 6 cut(s) 75, 172, 287, 397, 536, 970
LmnI GCTCC 2 cut(s) 262, 293
Lsp1109I GCAGC 3 cut(s) 45, 198, 294
LweI GCATC 3 cut(s) 626, 715, 974
MaeI CTAG 2 cut(s) 696, 1023
MaeII ACGT 1 cut(s) 891
MaeIII GTNAC 1 cut(s) 1161
MalI GATC 6 cut(s) 77, 174, 289, 399, 538, 972
MboI GATC 6 cut(s) 75, 172, 287, 397, 536, 970
MboII GAAGA 4 cut(s) 97, 375, 794, 887
MfeI CAATTG 2 cut(s) 597, 1194
MflI RGATCY 2 cut(s) 287, 970
MhlI GDGCHC 3 cut(s) 259, 431, 528
MlsI TGGCCA 1 cut(s) 510
MluNI TGGCCA 1 cut(s) 510
Mly113I GGCGCC 1 cut(s) 234
MlyI GAGTC 5 cut(s) 21, 143, 199, 364, 1211
MmeI TCCRAC 2 cut(s) 150, 200
Mox20I TGGCCA 1 cut(s) 510
MroI TCCGGA 1 cut(s) 290
MroXI GAANNNNTTC 1 cut(s) 1079
MscI TGGCCA 1 cut(s) 510
MseI TTAA 4 cut(s) 459, 756, 885, 1224
MslI CAYNNNNRTG 3 cut(s) 672, 902, 1261
Msp20I TGGCCA 1 cut(s) 510
MspI CCGG 5 cut(s) 153, 261, 291, 417, 766
MspR9I CCNGG 3 cut(s) 261, 262, 418
MunI CAATTG 2 cut(s) 597, 1194
MvnI CGCG 2 cut(s) 138, 497
MwoI GCNNNNNNNGC 5 cut(s) 142, 240, 503, 620, 1023
NarI GGCGCC 1 cut(s) 234
NciI CCSGG 3 cut(s) 261, 262, 418
NdeI CATATG 1 cut(s) 1284
NdeII GATC 6 cut(s) 75, 172, 287, 397, 536, 970
NlaIII CATG 9 cut(s) 354, 397, 583, 775, 980, 1122, 1143, 1158, 1266
NlaIV GGNNCC 5 cut(s) 235, 266, 289, 295, 852
NmuCI GTSAC 1 cut(s) 1161
OliI CACNNNNGTG 1 cut(s) 902
PdmI GAANNNNTTC 1 cut(s) 1079
PfeI GAWTC 2 cut(s) 347, 701
PflMI CCANNNNNTGG 2 cut(s) 132, 303
PfoI TCCNGGA 1 cut(s) 416
PkrI GCNGC 4 cut(s) 35, 187, 213, 309
PleI GAGTC 5 cut(s) 21, 143, 199, 364, 1210
PluTI GGCGCC 1 cut(s) 237
PpsI GAGTC 5 cut(s) 21, 143, 199, 364, 1210
PsiI TTATAA 1 cut(s) 1112
Psp124BI GAGCTC 1 cut(s) 431
PspFI CCCAGC 1 cut(s) 919
PspN4I GGNNCC 5 cut(s) 235, 266, 289, 295, 852
PspPI GGNCC 2 cut(s) 57, 768
PstNI CAGNNNCTG 1 cut(s) 1205
PsuI RGATCY 2 cut(s) 287, 970
RsaI GTAC 4 cut(s) 266, 392, 485, 1220
RsaNI GTAC 4 cut(s) 265, 391, 484, 1219
RseI CAYNNNNRTG 3 cut(s) 672, 902, 1261
SacI GAGCTC 1 cut(s) 431
SalI GTCGAC 1 cut(s) 197
SaqAI TTAA 4 cut(s) 459, 756, 885, 1224
SatI GCNGC 4 cut(s) 34, 186, 212, 308
Sau3AI GATC 6 cut(s) 75, 172, 287, 397, 536, 970
Sau96I GGNCC 2 cut(s) 57, 768
SchI GAGTC 5 cut(s) 21, 143, 199, 364, 1211
ScrFI CCNGG 3 cut(s) 261, 262, 418
SduI GDGCHC 3 cut(s) 259, 431, 528
SfaNI GCATC 3 cut(s) 626, 715, 974
SfoI GGCGCC 1 cut(s) 235
SinI GGWCC 1 cut(s) 768
SmaI CCCGGG 1 cut(s) 262
SmiMI CAYNNNNRTG 3 cut(s) 672, 902, 1261
SsiI CCGC 5 cut(s) 5, 31, 183, 186, 974
SspDI GGCGCC 1 cut(s) 233
SspMI CTAG 2 cut(s) 696, 1023
SstI GAGCTC 1 cut(s) 431
StyD4I CCNGG 3 cut(s) 259, 260, 416
StyI CCWWGG 1 cut(s) 695
TaaI ACNGT 4 cut(s) 483, 488, 671, 709
TaiI ACGT 1 cut(s) 894
TaqI TCGA 5 cut(s) 171, 198, 682, 741, 1228
TatI WGTACW 1 cut(s) 390
TauI GCSGC 1 cut(s) 188
TfiI GAWTC 2 cut(s) 347, 701
Tru1I TTAA 4 cut(s) 459, 756, 885, 1224
Tru9I TTAA 4 cut(s) 459, 756, 885, 1224
TscAI CASTG 4 cut(s) 674, 909, 1044, 1077
TseFI GTSAC 1 cut(s) 1161
TseI GCWGC 3 cut(s) 33, 211, 307
Tsp45I GTSAC 1 cut(s) 1161
TspDTI ATGAA 5 cut(s) 88, 600, 1107, 1118, 1301
TspGWI ACGGA 2 cut(s) 399, 1040
TspMI CCCGGG 1 cut(s) 260
TspRI CASTG 4 cut(s) 674, 909, 1044, 1077
Van91I CCANNNNNTGG 2 cut(s) 132, 303
VpaK11BI GGWCC 1 cut(s) 768
XapI RAATTY 3 cut(s) 1079, 1114, 1288
XmaI CCCGGG 1 cut(s) 260
XmaJI CCTAGG 1 cut(s) 695
XmiI GTMKAC 1 cut(s) 198
XmnI GAANNNNTTC 1 cut(s) 1079
XspI CTAG 2 cut(s) 696, 1023
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.