AT3G09410

Hydrolyzes acetyl esters in homogalacturonan regions of pectin. In type I primary cell wall, galacturonic acid residues of pectin can be acetylated at the O-2 and O-3 positions. Decreasing the degree of acetylation of pectin gels in vitro alters their physical properties

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0001587
Physical Location & Seq
Reverse (-)
2897638 .. 2901055
3418 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G09410.3

Sequence Viewer

Length: 1284 bp
ATGGCGATTCCAAGGTTTAGCTCTCTGTTACGGTGTAGAAAATGGGCTAAAAGTGACTGGCTTGTCGCTTCTATAGGATGCGTATTGATCGTTTTTTTCCTCTCTTTCTTCTTCGATCCCACTTCAGACTCTGTCCCCTCCGTTGACCGATCTCGGCCAATCATATCTCCTAGCGATCTGGTCAAGCTGAAGTTGTCGAGTGTAGCTAAAGAGAGAGGAGCTTTTTGTTTGGATGGAAGCTTGCCTGGGTACCACTTTCATGAGGGTTCTGGATCTGGCTCCCAAAGCTGGCTTGTTCATTTGGAGGGTGGAGGCTGGTGCAACACAGTTGCGTCATGTTCTGCTCGAGCATTGACTAAATTAGGTTCTTCCAACTACTTTGAACAAGAAGTGGCCTTTCAAGGTGTTTTAAGTAGTGACCCATCTCAAAATCCTGAATTCTTTAACTGGAACAAGGTTGCGATAAGGTACTGTGATGGTGCTTCTTTTTCTGGACGCCCTGAAGCTGAATTCAAGAATGGCACACGGCTTTTCTTCCGGGGCCAGCTTATTTGGGAGGCTATTATTGATGAACTTTTGTCAATGGGCATGTCAGATGCCAAACAGGCCATACTTACAGGATGTTCTGCCGGTGGCTTGGCAAGTCTTATACATTGTGACTACTTTCGAGATCATCTTCCAAAAGATGCAGCTGTCAAATGTGTTTCTGATGGAGGCTACTTTCTCAACGTGCCTGATGTCCTTGGAAACCCAACAATGAGGTCTTTCTATCATGATGTTGTTAATTTGCAGGGCGTAGAGAAGAGCTTGGATCAGAAATGTGTAGCCAAAACGGAGCCATCTAAGTGTATGTTTCCTCAAGAATTCCTGAAAAACATAAGAACTCCGGTATTTCTTGTCAATCCAGCCTACGATTTTTGGCAGATTCAACATGTCTTGGTACCAACTTCTGCGGATCCAGATAAAAGTTGGGCAAAGTGCAGACTCAATATTAAGGAATGTGATGCCGAACAGATTAAAGTTCTACACGGTTTTCGCAGTTCTATGATGACTGCAATTGGGGAATTTCATCAGAACAAAGATGGTGGGATGTTCATAGATTCCTGCTATGCTCATTGCCAGACAGTCATGTCCGTGACATGGCACTCCCTGACCTCACCAAGAATCGAAAATAAGACAATTGCAGAGTCTGTAGGTGACTGGTACTTCAACAGAAAACCGGTGAAGCTAATCGATTGTCCATACCCGTGCAATCCTTCTTGTTACAACATGAATTTCACATGA

Protein Analysis

427

Amino Acids

47.64

Weight (kDa)

6.81

Isoelectric Point (pI)

42.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PAE PF03283 59 - 392 9.6e-143 Pectinacetylesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 62, 1129
Acc65I GGTACC 2 cut(s) 249, 940
AccB1I GGYRCC 2 cut(s) 249, 940
AciI CCGC 1 cut(s) 953
AclWI GGATC 5 cut(s) 110, 280, 819, 950, 963
AcoI YGGCCR 1 cut(s) 155
AcsI RAATTY 5 cut(s) 437, 509, 863, 1064, 1273
AcuI CTGAAG 3 cut(s) 108, 209, 522
AcyI GRCGYC 1 cut(s) 496
AfaI GTAC 4 cut(s) 251, 470, 942, 1205
AfiI CCNNNNNNNGG 2 cut(s) 288, 1140
AflIII ACRYGT 1 cut(s) 931
AgeI ACCGGT 1 cut(s) 1219
AgsI TTSAA 5 cut(s) 383, 401, 514, 929, 1210
AjnI CCWGG 1 cut(s) 244
AlwI GGATC 5 cut(s) 110, 280, 819, 950, 963
AlwNI CAGNNNCTG 2 cut(s) 131, 1190
Ama87I CYCGRG 1 cut(s) 345
AoxI GGCC 4 cut(s) 155, 393, 541, 606
ApeKI GCWGC 1 cut(s) 689
ApoI RAATTY 5 cut(s) 437, 509, 863, 1064, 1273
AsiGI ACCGGT 1 cut(s) 1219
Asp718I GGTACC 2 cut(s) 249, 940
AspS9I GGNCC 1 cut(s) 541
AsuC2I CCSGG 1 cut(s) 539
AsuHPI GGTGA 3 cut(s) 1149, 1208, 1234
AvaI CYCGRG 1 cut(s) 345
BamHI GGATCC 1 cut(s) 955
BanI GGYRCC 2 cut(s) 249, 940
BbvI GCAGC 1 cut(s) 701
BccI CCATC 6 cut(s) 227, 430, 470, 704, 847, 1076
BceAI ACGGC 1 cut(s) 542
BciT130I CCWGG 1 cut(s) 246
BcnI CCSGG 1 cut(s) 539
BfaI CTAG 1 cut(s) 171
BfmI CTRYAG 2 cut(s) 72, 1191
BglI GCCNNNNNGGC 1 cut(s) 605
BisI GCNGC 1 cut(s) 690
BlsI GCNGC 1 cut(s) 691
Bme1390I CCNGG 2 cut(s) 246, 539
BmeT110I CYCGRG 1 cut(s) 345
BmgT120I GGNCC 1 cut(s) 541
BmiI GGNNCC 6 cut(s) 251, 280, 542, 837, 942, 957
BmrFI CCNGG 2 cut(s) 246, 539
BmsI GCATC 4 cut(s) 68, 586, 676, 994
BpuEI CTTGAG 1 cut(s) 843
BpuMI CCSGG 1 cut(s) 539
Bsa29I ATCGAT 1 cut(s) 1233
BsaHI GRCGYC 1 cut(s) 496
BsaJI CCNNGG 4 cut(s) 11, 245, 538, 742
BsaWI WCCGGW 2 cut(s) 886, 1219
Bsc4I CCNNNNNNNGG 2 cut(s) 288, 1140
Bse118I RCCGGY 2 cut(s) 629, 1219
Bse1I ACTGG 3 cut(s) 62, 452, 1205
Bse3DI GCAATG 1 cut(s) 1114
BseBI CCWGG 1 cut(s) 246
BseCI ATCGAT 1 cut(s) 1233
BseDI CCNNGG 4 cut(s) 11, 245, 538, 742
BseGI GGATG 4 cut(s) 83, 238, 626, 1095
BseLI CCNNNNNNNGG 2 cut(s) 288, 1140
BseMI GCAATG 1 cut(s) 1114
BseNI ACTGG 3 cut(s) 62, 452, 1205
BseRI GAGGAG 1 cut(s) 231
BseXI GCAGC 1 cut(s) 701
BsgI GTGCAG 1 cut(s) 1000
BshFI GGCC 4 cut(s) 157, 395, 543, 608
BshNI GGYRCC 2 cut(s) 249, 940
BshTI ACCGGT 1 cut(s) 1219
BshVI ATCGAT 1 cut(s) 1233
BsiHKCI CYCGRG 1 cut(s) 345
BsiSI CCGG 4 cut(s) 538, 630, 887, 1220
BslFI GGGAC 1 cut(s) 119
BslI CCNNNNNNNGG 2 cut(s) 288, 1140
BsmFI GGGAC 1 cut(s) 119
BsnI GGCC 4 cut(s) 157, 395, 543, 608
BsoBI CYCGRG 1 cut(s) 345
Bsp143I GATC 8 cut(s) 87, 115, 149, 175, 272, 670, 811, 955
BspACI CCGC 1 cut(s) 953
BspANI GGCC 4 cut(s) 157, 395, 543, 608
BspDI ATCGAT 1 cut(s) 1233
BspHI TCATGA 2 cut(s) 259, 772
BspLI GGNNCC 6 cut(s) 251, 280, 542, 837, 942, 957
BspPI GGATC 5 cut(s) 110, 280, 819, 950, 963
BspQI GCTCTTC 1 cut(s) 797
BspT107I GGYRCC 2 cut(s) 249, 940
BsrDI GCAATG 1 cut(s) 1114
BsrFI RCCGGY 2 cut(s) 629, 1219
BsrI ACTGG 3 cut(s) 62, 452, 1205
BssAI RCCGGY 2 cut(s) 629, 1219
BssECI CCNNGG 4 cut(s) 11, 245, 538, 742
BssMI GATC 8 cut(s) 87, 115, 149, 175, 272, 670, 811, 955
BssNI GRCGYC 1 cut(s) 496
BssT1I CCWWGG 2 cut(s) 11, 742
Bst2UI CCWGG 1 cut(s) 246
Bst4CI ACNGT 5 cut(s) 33, 328, 473, 1031, 1126
Bst6I CTCTTC 1 cut(s) 797
BstACI GRCGYC 1 cut(s) 496
BstC8I GCNNGC 3 cut(s) 242, 290, 545
BstDEI CTNAG 1 cut(s) 843
BstF5I GGATG 4 cut(s) 83, 238, 626, 1095
BstKTI GATC 8 cut(s) 90, 118, 152, 178, 275, 673, 814, 958
BstMBI GATC 8 cut(s) 87, 115, 149, 175, 272, 670, 811, 955
BstMWI GCNNNNNNNGC 2 cut(s) 285, 605
BstNI CCWGG 1 cut(s) 246
BstNSI RCATGY 2 cut(s) 592, 935
BstSCI CCNGG 2 cut(s) 244, 537
BstSFI CTRYAG 2 cut(s) 72, 1191
BstV1I GCAGC 1 cut(s) 701
BstX2I RGATCY 2 cut(s) 272, 955
BstYI RGATCY 2 cut(s) 272, 955
Bsu15I ATCGAT 1 cut(s) 1233
BsuRI GGCC 4 cut(s) 157, 395, 543, 608
BsuTUI ATCGAT 1 cut(s) 1233
BtsCI GGATG 4 cut(s) 83, 238, 626, 1095
Cac8I GCNNGC 3 cut(s) 242, 290, 545
CaiI CAGNNNCTG 2 cut(s) 131, 1190
CciI TCATGA 2 cut(s) 259, 772
Cfr10I RCCGGY 2 cut(s) 629, 1219
Cfr13I GGNCC 1 cut(s) 541
ClaI ATCGAT 1 cut(s) 1233
CseI GACGC 2 cut(s) 321, 504
Csp6I GTAC 4 cut(s) 250, 469, 941, 1204
CspAI ACCGGT 1 cut(s) 1219
CspCI CAANNNNNGTGG 2 cut(s) 1066, 1101
CviAII CATG 9 cut(s) 260, 336, 589, 773, 932, 1129, 1140, 1270, 1281
CviQI GTAC 4 cut(s) 250, 469, 941, 1204
DdeI CTNAG 1 cut(s) 843
DpnI GATC 8 cut(s) 89, 117, 151, 177, 274, 672, 813, 957
DpnII GATC 8 cut(s) 87, 115, 149, 175, 272, 670, 811, 955
DrdI GACNNNNNNGTC 2 cut(s) 62, 1129
DseDI GACNNNNNNGTC 2 cut(s) 62, 1129
EaeI YGGCCR 1 cut(s) 155
Eam1104I CTCTTC 1 cut(s) 797
EarI CTCTTC 1 cut(s) 797
Eco130I CCWWGG 2 cut(s) 11, 742
Eco57I CTGAAG 3 cut(s) 108, 209, 522
Eco88I CYCGRG 1 cut(s) 345
EcoRI GAATTC 3 cut(s) 437, 509, 863
EcoRII CCWGG 1 cut(s) 244
EcoT14I CCWWGG 2 cut(s) 11, 742
ErhI CCWWGG 2 cut(s) 11, 742
FaeI CATG 9 cut(s) 263, 339, 592, 776, 935, 1132, 1143, 1273, 1284
FaqI GGGAC 1 cut(s) 119
FatI CATG 9 cut(s) 259, 335, 588, 772, 931, 1128, 1139, 1269, 1280
Fnu4HI GCNGC 1 cut(s) 690
FokI GGATG 4 cut(s) 90, 245, 633, 1102
Fsp4HI GCNGC 1 cut(s) 690
FspBI CTAG 1 cut(s) 171
GluI GCNGC 1 cut(s) 690
HaeIII GGCC 4 cut(s) 157, 395, 543, 608
HapII CCGG 4 cut(s) 538, 630, 887, 1220
HgaI GACGC 2 cut(s) 321, 504
Hin1I GRCGYC 1 cut(s) 496
Hin1II CATG 9 cut(s) 263, 339, 592, 776, 935, 1132, 1143, 1273, 1284
HincII GTYRAC 1 cut(s) 145
HindII GTYRAC 1 cut(s) 145
HindIII AAGCTT 1 cut(s) 238
HinfI GANTC 7 cut(s) 7, 128, 925, 984, 1100, 1164, 1187
HpaII CCGG 4 cut(s) 538, 630, 887, 1220
HphI GGTGA 3 cut(s) 1149, 1208, 1234
Hpy166II GTNNAC 1 cut(s) 145
Hpy188I TCNGA 5 cut(s) 127, 595, 709, 816, 1074
Hpy8I GTNNAC 1 cut(s) 145
HpyAV CCTTC 1 cut(s) 1266
HpyCH4III ACNGT 5 cut(s) 33, 328, 473, 1031, 1126
HpyCH4IV ACGT 1 cut(s) 729
HpyCH4V TGCA 7 cut(s) 321, 689, 790, 981, 1055, 1184, 1251
HpyF10VI GCNNNNNNNGC 2 cut(s) 285, 605
HpyF3I CTNAG 1 cut(s) 843
HpySE526I ACGT 1 cut(s) 729
Hsp92I GRCGYC 1 cut(s) 496
Hsp92II CATG 9 cut(s) 263, 339, 592, 776, 935, 1132, 1143, 1273, 1284
KpnI GGTACC 2 cut(s) 253, 944
Kzo9I GATC 8 cut(s) 87, 115, 149, 175, 272, 670, 811, 955
LguI GCTCTTC 1 cut(s) 797
LmnI GCTCC 3 cut(s) 218, 284, 835
Lsp1109I GCAGC 1 cut(s) 701
LweI GCATC 4 cut(s) 68, 586, 676, 994
MaeI CTAG 1 cut(s) 171
MaeII ACGT 1 cut(s) 729
MaeIII GTNAC 7 cut(s) 27, 53, 416, 656, 1135, 1196, 1262
MalI GATC 8 cut(s) 89, 117, 151, 177, 274, 672, 813, 957
MboI GATC 8 cut(s) 87, 115, 149, 175, 272, 670, 811, 955
MboII GAAGA 6 cut(s) 100, 103, 360, 526, 668, 814
MfeI CAATTG 2 cut(s) 1056, 1179
MflI RGATCY 2 cut(s) 272, 955
MluCI AATT 9 cut(s) 359, 437, 509, 784, 863, 1056, 1064, 1179, 1273
MlyI GAGTC 3 cut(s) 122, 978, 1196
MmeI TCCRAC 1 cut(s) 396
MseI TTAA 5 cut(s) 410, 444, 783, 993, 1017
MslI CAYNNNNRTG 4 cut(s) 258, 844, 1133, 1246
MspA1I CMGCKG 1 cut(s) 692
MspI CCGG 4 cut(s) 538, 630, 887, 1220
MspR9I CCNGG 2 cut(s) 246, 539
MunI CAATTG 2 cut(s) 1056, 1179
MvaI CCWGG 1 cut(s) 246
MwoI GCNNNNNNNGC 2 cut(s) 285, 605
NciI CCSGG 1 cut(s) 539
NdeII GATC 8 cut(s) 87, 115, 149, 175, 272, 670, 811, 955
NlaIII CATG 9 cut(s) 263, 339, 592, 776, 935, 1132, 1143, 1273, 1284
NlaIV GGNNCC 6 cut(s) 251, 280, 542, 837, 942, 957
NmeAIII GCCGAG 1 cut(s) 133
NmuCI GTSAC 5 cut(s) 53, 416, 656, 1135, 1196
NspI RCATGY 2 cut(s) 592, 935
PaeR7I CTCGAG 1 cut(s) 345
PagI TCATGA 2 cut(s) 259, 772
PciI ACATGT 1 cut(s) 931
PciSI GCTCTTC 1 cut(s) 797
PfeI GAWTC 4 cut(s) 7, 925, 1100, 1164
PflFI GACNNNGTC 1 cut(s) 131
PinAI ACCGGT 1 cut(s) 1219
PkrI GCNGC 1 cut(s) 691
PleI GAGTC 3 cut(s) 122, 978, 1195
PpsI GAGTC 3 cut(s) 122, 978, 1195
PscI ACATGT 1 cut(s) 931
Psp6I CCWGG 1 cut(s) 244
PspGI CCWGG 1 cut(s) 244
PspN4I GGNNCC 6 cut(s) 251, 280, 542, 837, 942, 957
PspPI GGNCC 1 cut(s) 541
PspXI VCTCGAGB 1 cut(s) 345
PstNI CAGNNNCTG 2 cut(s) 131, 1190
PsuI RGATCY 2 cut(s) 272, 955
PsyI GACNNNGTC 1 cut(s) 131
PvuII CAGCTG 1 cut(s) 692
RsaI GTAC 4 cut(s) 251, 470, 942, 1205
RsaNI GTAC 4 cut(s) 250, 469, 941, 1204
RseI CAYNNNNRTG 4 cut(s) 258, 844, 1133, 1246
SapI GCTCTTC 1 cut(s) 797
SaqAI TTAA 5 cut(s) 410, 444, 783, 993, 1017
SatI GCNGC 1 cut(s) 690
Sau3AI GATC 8 cut(s) 87, 115, 149, 175, 272, 670, 811, 955
Sau96I GGNCC 1 cut(s) 541
SchI GAGTC 3 cut(s) 122, 978, 1196
ScrFI CCNGG 2 cut(s) 246, 539
SfaNI GCATC 4 cut(s) 68, 586, 676, 994
SfcI CTRYAG 2 cut(s) 72, 1191
Sfr274I CTCGAG 1 cut(s) 345
SlaI CTCGAG 1 cut(s) 345
SmiMI CAYNNNNRTG 4 cut(s) 258, 844, 1133, 1246
SmlI CTYRAG 2 cut(s) 345, 858
SmoI CTYRAG 2 cut(s) 345, 858
Sse9I AATT 9 cut(s) 359, 437, 509, 784, 863, 1056, 1064, 1179, 1273
SsiI CCGC 1 cut(s) 953
SspI AATATT 1 cut(s) 991
SspMI CTAG 1 cut(s) 171
StyD4I CCNGG 2 cut(s) 244, 537
StyI CCWWGG 2 cut(s) 11, 742
TaaI ACNGT 5 cut(s) 33, 328, 473, 1031, 1126
TaiI ACGT 1 cut(s) 732
TaqI TCGA 6 cut(s) 114, 197, 346, 667, 1167, 1233
TaqII GACCGA 1 cut(s) 162
TasI AATT 9 cut(s) 359, 437, 509, 784, 863, 1056, 1064, 1179, 1273
TfiI GAWTC 4 cut(s) 7, 925, 1100, 1164
Tru1I TTAA 5 cut(s) 410, 444, 783, 993, 1017
Tru9I TTAA 5 cut(s) 410, 444, 783, 993, 1017
TseFI GTSAC 5 cut(s) 53, 416, 656, 1135, 1196
TseI GCWGC 1 cut(s) 689
Tsp45I GTSAC 5 cut(s) 53, 416, 656, 1135, 1196
TspDTI ATGAA 5 cut(s) 248, 287, 585, 1058, 1084
TspGWI ACGGA 3 cut(s) 130, 848, 1123
Tth111I GACNNNGTC 1 cut(s) 131
XapI RAATTY 5 cut(s) 437, 509, 863, 1064, 1273
XceI RCATGY 2 cut(s) 592, 935
XcmI CCANNNNNNNNNTGG 1 cut(s) 966
XhoI CTCGAG 1 cut(s) 345
XspI CTAG 1 cut(s) 171
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.