Prupe.6G254300_v2.0.a1

Hydrolyzes acetyl esters in homogalacturonan regions of pectin. In type I primary cell wall, galacturonic acid residues of pectin can be acetylated at the O-2 and O-3 positions. Decreasing the degree of acetylation of pectin gels in vitro alters their physical properties

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp06
OG0001587
Physical Location & Seq
Forward (+)
24743372 .. 24747954
4583 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.6G254300.3

Sequence Viewer

Length: 1221 bp
ATGGCGGTGGAGAATTTGGCTTCAAATTCAATGGCGAATATTTCTTGGCTTCACGGCCTGCTATGGTGGAGAAACTGGGGCAAAAGAGAATGGGCAATATCAGCCATCGGATTTGCCGTCTTTGTCTTCGCTCTCGCTTTCGTCTCCTACTCTTGGATCTCTGATCCAGACTCCAACCTCAGCTCCTCATTCCATCGAATCACCGCCACCGATTTGGTCGACTTAACCTTGTTGCACAACGCCACACGTAGAGGCGCAGTTTGCTTAGATGGGAGTTTGCCTGGCTACCATTATCAAAAGGGCTTTGGTTCTGGCGCCAATAGTTGGGTGCTTTACATTGAGGGTGGAGGTTGGTGCAATACAATAGAATCGTGTTTTTGGCGTAGAGAGATCCATTTAGGGTCTTCCAAACATATGGGACATCGAGTTCACTTTACTGGGATTTTAAGCCCTCACCAAGCACAAAATCCTGAATTCTTTAACTGGAACAGAGTCAAAGTGCAGTATTGTGATGGTGCATCTTTAGCCGGCCACCCGGATAAGGAGTTGAGGAATGGAACACGTCTTTTCTTTAGGGGCCAGCTCATCTGGGAAGCACTTATGGATAAGTTCTTCTCACTTGGCCTGTCGAAGGCGAAACAGGCCCTTCTTGCAGGATGCTCCGCTGGTGGATTGGCGGCTCTTATACATTGTGATGAATTTCGTGGTCTTCTGCCTAAGAATGGTTTAAGAAAAAGTTTACACAAGGATTGTATTGCAAGAAAGGAACCAGCTGAGTGTCTCTTTCCTAAAGAAATTATTAAAAACATTGCCACCCCAGTGTTCCTCCTCCACTCAGCTTATGATTTTTGGCAGATACAAAATATCTTGATACCTGAAGGATCAGATCTGTATCACTACTGGCAAAAGTGCAGACTGGACATTCATAATTGCAATGCTACCCAGGTTGAAATACTGAAAGGTTACCGCAGATCTCTTTTGAAGGCGCTAAAAGAATTCCAGAAAAATAAGGAAGGAGGAATGTTTATAAGTTCATGCTTTATTCATTGCCAGGCATGGATGACTGAGACATGGCATGCTCCTACTTCTCCACGAATAAACAATAAGACAATTGCAGAGTCCGTTGGCGATTGGTACTTTAATCGAAATGCATCAAAGAAAATTGACTGCCCGTTTCTATGCAATCCTACCTGCTATCATGTGAATTTCACACACGGTTGA

Protein Analysis

407

Amino Acids

46.14

Weight (kDa)

8.89

Isoelectric Point (pI)

42.74

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1028
Acc36I ACCTGC 1 cut(s) 1199
AccB1I GGYRCC 1 cut(s) 314
AccB7I CCANNNNNTGG 1 cut(s) 324
AccI GTMKAC 1 cut(s) 219
AciI CCGC 5 cut(s) 5, 204, 663, 677, 967
AclWI GGATC 4 cut(s) 158, 164, 385, 889
AcoI YGGCCR 1 cut(s) 529
AcsI RAATTY 6 cut(s) 13, 25, 473, 698, 995, 1204
AcuI CTGAAG 1 cut(s) 897
AcyI GRCGYC 1 cut(s) 315
AfaI GTAC 1 cut(s) 1136
AfiI CCNNNNNNNGG 5 cut(s) 153, 324, 541, 631, 722
AflIII ACRYGT 2 cut(s) 245, 560
AgsI TTSAA 4 cut(s) 24, 30, 950, 982
AjiI CACGTC 1 cut(s) 563
AjnI CCWGG 3 cut(s) 280, 942, 1050
AleI CACNNNNGTG 1 cut(s) 818
AloI GAACNNNNNNTCC 2 cut(s) 411, 443
AluBI AGCT 4 cut(s) 183, 583, 773, 839
AluI AGCT 4 cut(s) 183, 583, 773, 839
Alw26I GTCTC 3 cut(s) 148, 785, 1061
AlwI GGATC 4 cut(s) 158, 164, 385, 889
AoxI GGCC 5 cut(s) 55, 529, 577, 622, 642
ApoI RAATTY 6 cut(s) 13, 25, 473, 698, 995, 1204
AspLEI GCGC 3 cut(s) 257, 317, 988
AspS9I GGNCC 2 cut(s) 577, 643
AsuC2I CCSGG 1 cut(s) 536
AsuHPI GGTGA 2 cut(s) 193, 446
BanI GGYRCC 1 cut(s) 314
BbsI GAAGAC 3 cut(s) 118, 396, 701
BbvCI CCTCAGC 1 cut(s) 179
BccI CCATC 4 cut(s) 113, 201, 263, 506
BceAI ACGGC 2 cut(s) 70, 101
BciT130I CCWGG 3 cut(s) 282, 944, 1052
BcnI CCSGG 1 cut(s) 536
BcoDI GTCTC 3 cut(s) 148, 785, 1061
BfoI RGCGCY 2 cut(s) 318, 989
BfuAI ACCTGC 1 cut(s) 1199
BglII AGATCT 2 cut(s) 886, 971
BisI GCNGC 1 cut(s) 678
BlsI GCNGC 1 cut(s) 679
Bme1390I CCNGG 4 cut(s) 282, 536, 944, 1052
BmgBI CACGTC 1 cut(s) 563
BmgT120I GGNCC 2 cut(s) 577, 643
BmiI GGNNCC 3 cut(s) 316, 578, 768
BmrFI CCNGG 4 cut(s) 282, 536, 944, 1052
BmrI ACTGGG 3 cut(s) 85, 447, 812
BmsI GCATC 3 cut(s) 527, 647, 1160
BmuI ACTGGG 3 cut(s) 85, 447, 812
BpiI GAAGAC 3 cut(s) 118, 396, 701
Bpu10I CCTNAGC 1 cut(s) 179
BpuMI CCSGG 1 cut(s) 536
BsaAI YACGTR 1 cut(s) 248
BsaBI GATNNNNATC 1 cut(s) 891
BsaHI GRCGYC 1 cut(s) 315
BsaJI CCNNGG 1 cut(s) 942
BsaXI ACNNNNNCTCC 2 cut(s) 167, 197
Bsc4I CCNNNNNNNGG 5 cut(s) 153, 324, 541, 631, 722
Bse118I RCCGGY 1 cut(s) 527
Bse1I ACTGG 6 cut(s) 80, 442, 488, 818, 905, 921
Bse3DI GCAATG 3 cut(s) 807, 940, 1045
Bse8I GATNNNNATC 1 cut(s) 891
BseBI CCWGG 3 cut(s) 282, 944, 1052
BseDI CCNNGG 1 cut(s) 942
BseGI GGATG 2 cut(s) 662, 1065
BseJI GATNNNNATC 1 cut(s) 891
BseLI CCNNNNNNNGG 5 cut(s) 153, 324, 541, 631, 722
BseMI GCAATG 3 cut(s) 807, 940, 1045
BseMII CTCAG 4 cut(s) 193, 765, 849, 1056
BseNI ACTGG 6 cut(s) 80, 442, 488, 818, 905, 921
BseRI GAGGAG 2 cut(s) 175, 818
BsgI GTGCAG 2 cut(s) 521, 931
BshFI GGCC 5 cut(s) 57, 531, 579, 624, 644
BshNI GGYRCC 1 cut(s) 314
BsiSI CCGG 2 cut(s) 528, 536
BslFI GGGAC 1 cut(s) 432
BslI CCNNNNNNNGG 5 cut(s) 153, 324, 541, 631, 722
BsmAI GTCTC 3 cut(s) 148, 785, 1061
BsmBI CGTCTC 1 cut(s) 148
BsmFI GGGAC 1 cut(s) 432
BsnI GGCC 5 cut(s) 57, 531, 579, 624, 644
Bsp143I GATC 6 cut(s) 156, 163, 390, 881, 886, 971
BspACI CCGC 5 cut(s) 5, 204, 663, 677, 967
BspANI GGCC 5 cut(s) 57, 531, 579, 624, 644
BspCNI CTCAG 4 cut(s) 192, 766, 848, 1057
BspLI GGNNCC 3 cut(s) 316, 578, 768
BspMI ACCTGC 1 cut(s) 1199
BspPI GGATC 4 cut(s) 158, 164, 385, 889
BspT107I GGYRCC 1 cut(s) 314
BsrDI GCAATG 3 cut(s) 807, 940, 1045
BsrFI RCCGGY 1 cut(s) 527
BsrI ACTGG 6 cut(s) 80, 442, 488, 818, 905, 921
BssAI RCCGGY 1 cut(s) 527
BssECI CCNNGG 1 cut(s) 942
BssMI GATC 6 cut(s) 156, 163, 390, 881, 886, 971
BssNI GRCGYC 1 cut(s) 315
Bst2UI CCWGG 3 cut(s) 282, 944, 1052
Bst4CI ACNGT 1 cut(s) 1217
BstACI GRCGYC 1 cut(s) 315
BstBAI YACGTR 1 cut(s) 248
BstC8I GCNNGC 4 cut(s) 59, 529, 581, 1077
BstDEI CTNAG 6 cut(s) 179, 265, 717, 774, 835, 1065
BstEII GGTNACC 1 cut(s) 962
BstENI CCTNNNNNAGG 1 cut(s) 629
BstF5I GGATG 2 cut(s) 662, 1065
BstH2I RGCGCY 2 cut(s) 318, 989
BstHHI GCGC 3 cut(s) 257, 317, 988
BstKTI GATC 6 cut(s) 159, 166, 393, 884, 889, 974
BstMAI GTCTC 3 cut(s) 148, 785, 1061
BstMBI GATC 6 cut(s) 156, 163, 390, 881, 886, 971
BstMWI GCNNNNNNNGC 5 cut(s) 101, 261, 524, 641, 650
BstNI CCWGG 3 cut(s) 282, 944, 1052
BstNSI RCATGY 1 cut(s) 1079
BstPI GGTNACC 1 cut(s) 962
BstSCI CCNGG 4 cut(s) 280, 534, 942, 1050
BstV2I GAAGAC 3 cut(s) 118, 396, 701
BstX2I RGATCY 4 cut(s) 156, 390, 886, 971
BstXI CCANNNNNNTGG 2 cut(s) 214, 415
BstYI RGATCY 4 cut(s) 156, 390, 886, 971
BsuRI GGCC 5 cut(s) 57, 531, 579, 624, 644
BtrI CACGTC 1 cut(s) 563
BtsCI GGATG 2 cut(s) 662, 1065
BtsIMutI CAGTG 1 cut(s) 825
BveI ACCTGC 1 cut(s) 1199
Cac8I GCNNGC 4 cut(s) 59, 529, 581, 1077
CfoI GCGC 3 cut(s) 257, 317, 988
Cfr10I RCCGGY 1 cut(s) 527
Cfr13I GGNCC 2 cut(s) 577, 643
Csp6I GTAC 1 cut(s) 1135
CviAII CATG 5 cut(s) 1035, 1056, 1071, 1076, 1199
CviQI GTAC 1 cut(s) 1135
DdeI CTNAG 6 cut(s) 179, 265, 717, 774, 835, 1065
DinI GGCGCC 1 cut(s) 316
DpnI GATC 6 cut(s) 158, 165, 392, 883, 888, 973
DpnII GATC 6 cut(s) 156, 163, 390, 881, 886, 971
EaeI YGGCCR 1 cut(s) 529
Eco57I CTGAAG 1 cut(s) 897
Eco91I GGTNACC 1 cut(s) 962
EcoNI CCTNNNNNAGG 1 cut(s) 629
EcoO109I RGGNCCY 1 cut(s) 643
EcoO65I GGTNACC 1 cut(s) 962
EcoRI GAATTC 2 cut(s) 473, 995
EcoRII CCWGG 3 cut(s) 280, 942, 1050
EcoT22I ATGCAT 1 cut(s) 1153
EgeI GGCGCC 1 cut(s) 316
EheI GGCGCC 1 cut(s) 316
Esp3I CGTCTC 1 cut(s) 148
FaeI CATG 5 cut(s) 1038, 1059, 1074, 1079, 1202
FaqI GGGAC 1 cut(s) 432
FatI CATG 5 cut(s) 1034, 1055, 1070, 1075, 1198
FauNDI CATATG 1 cut(s) 414
FblI GTMKAC 1 cut(s) 219
Fnu4HI GCNGC 1 cut(s) 678
FokI GGATG 2 cut(s) 669, 1072
Fsp4HI GCNGC 1 cut(s) 678
GlaI GCGC 3 cut(s) 256, 316, 987
GluI GCNGC 1 cut(s) 678
HaeII RGCGCY 2 cut(s) 318, 989
HaeIII GGCC 5 cut(s) 57, 531, 579, 624, 644
HapII CCGG 2 cut(s) 528, 536
HhaI GCGC 3 cut(s) 257, 317, 988
Hin1I GRCGYC 1 cut(s) 315
Hin1II CATG 5 cut(s) 1038, 1059, 1074, 1079, 1202
Hin6I GCGC 3 cut(s) 255, 315, 986
HinP1I GCGC 3 cut(s) 255, 315, 986
HincII GTYRAC 1 cut(s) 220
HindII GTYRAC 1 cut(s) 220
HinfI GANTC 5 cut(s) 170, 198, 368, 492, 1118
HpaII CCGG 2 cut(s) 528, 536
HphI GGTGA 2 cut(s) 193, 446
Hpy166II GTNNAC 3 cut(s) 220, 430, 740
Hpy188I TCNGA 3 cut(s) 110, 163, 886
Hpy188III TCNNGA 4 cut(s) 167, 470, 868, 1000
Hpy8I GTNNAC 3 cut(s) 220, 430, 740
HpyAV CCTTC 5 cut(s) 625, 656, 872, 976, 1007
HpyCH4III ACNGT 1 cut(s) 1217
HpyCH4IV ACGT 2 cut(s) 247, 562
HpyF10VI GCNNNNNNNGC 5 cut(s) 101, 261, 524, 641, 650
HpyF3I CTNAG 6 cut(s) 179, 265, 717, 774, 835, 1065
HpySE526I ACGT 2 cut(s) 247, 562
Hsp92I GRCGYC 1 cut(s) 315
Hsp92II CATG 5 cut(s) 1038, 1059, 1074, 1079, 1202
HspAI GCGC 3 cut(s) 255, 315, 986
KasI GGCGCC 1 cut(s) 314
KroI GCCGGC 1 cut(s) 527
KroNI GCCGGC 1 cut(s) 529
Kzo9I GATC 6 cut(s) 156, 163, 390, 881, 886, 971
LmnI GCTCC 3 cut(s) 188, 665, 1084
LweI GCATC 3 cut(s) 527, 647, 1160
MaeII ACGT 2 cut(s) 247, 562
MaeIII GTNAC 1 cut(s) 962
MalI GATC 6 cut(s) 158, 165, 392, 883, 888, 973
MboI GATC 6 cut(s) 156, 163, 390, 881, 886, 971
MboII GAAGA 4 cut(s) 118, 396, 604, 701
MfeI CAATTG 1 cut(s) 1110
MflI RGATCY 4 cut(s) 156, 390, 886, 971
Mly113I GGCGCC 1 cut(s) 315
MlyI GAGTC 3 cut(s) 164, 501, 1127
MmeI TCCRAC 1 cut(s) 198
Mph1103I ATGCAT 1 cut(s) 1153
MroNI GCCGGC 1 cut(s) 527
MseI TTAA 6 cut(s) 224, 446, 480, 728, 801, 1140
MslI CAYNNNNRTG 2 cut(s) 693, 818
MspA1I CMGCKG 2 cut(s) 665, 773
MspI CCGG 2 cut(s) 528, 536
MspR9I CCNGG 4 cut(s) 282, 536, 944, 1052
MunI CAATTG 1 cut(s) 1110
MvaI CCWGG 3 cut(s) 282, 944, 1052
MwoI GCNNNNNNNGC 5 cut(s) 101, 261, 524, 641, 650
NaeI GCCGGC 1 cut(s) 529
NarI GGCGCC 1 cut(s) 315
NciI CCSGG 1 cut(s) 536
NdeI CATATG 1 cut(s) 414
NdeII GATC 6 cut(s) 156, 163, 390, 881, 886, 971
NgoMIV GCCGGC 1 cut(s) 527
NlaIII CATG 5 cut(s) 1038, 1059, 1074, 1079, 1202
NlaIV GGNNCC 3 cut(s) 316, 578, 768
NsiI ATGCAT 1 cut(s) 1153
NspI RCATGY 1 cut(s) 1079
OliI CACNNNNGTG 1 cut(s) 818
PaeI GCATGC 1 cut(s) 1079
PcsI WCGNNNNNNNCGW 1 cut(s) 114
PdiI GCCGGC 1 cut(s) 529
PfeI GAWTC 2 cut(s) 198, 368
PflMI CCANNNNNTGG 1 cut(s) 324
PkrI GCNGC 1 cut(s) 679
PleI GAGTC 3 cut(s) 164, 500, 1126
PluTI GGCGCC 1 cut(s) 318
PpsI GAGTC 3 cut(s) 164, 500, 1126
Ppu21I YACGTR 1 cut(s) 248
PsiI TTATAA 1 cut(s) 1028
Psp6I CCWGG 3 cut(s) 280, 942, 1050
PspEI GGTNACC 1 cut(s) 962
PspGI CCWGG 3 cut(s) 280, 942, 1050
PspN4I GGNNCC 3 cut(s) 316, 578, 768
PspPI GGNCC 2 cut(s) 577, 643
PsuI RGATCY 4 cut(s) 156, 390, 886, 971
PvuII CAGCTG 1 cut(s) 773
RsaI GTAC 1 cut(s) 1136
RsaNI GTAC 1 cut(s) 1135
RseI CAYNNNNRTG 2 cut(s) 693, 818
SalI GTCGAC 1 cut(s) 218
SaqAI TTAA 6 cut(s) 224, 446, 480, 728, 801, 1140
SatI GCNGC 1 cut(s) 678
Sau3AI GATC 6 cut(s) 156, 163, 390, 881, 886, 971
Sau96I GGNCC 2 cut(s) 577, 643
SchI GAGTC 3 cut(s) 164, 501, 1127
ScrFI CCNGG 4 cut(s) 282, 536, 944, 1052
SfaNI GCATC 3 cut(s) 527, 647, 1160
SfoI GGCGCC 1 cut(s) 316
SmiMI CAYNNNNRTG 2 cut(s) 693, 818
SphI GCATGC 1 cut(s) 1079
SsiI CCGC 5 cut(s) 5, 204, 663, 677, 967
SspDI GGCGCC 1 cut(s) 314
SspI AATATT 1 cut(s) 40
StyD4I CCNGG 4 cut(s) 280, 534, 942, 1050
TaaI ACNGT 1 cut(s) 1217
TaiI ACGT 2 cut(s) 250, 565
TaqI TCGA 5 cut(s) 196, 219, 424, 629, 1144
TauI GCSGC 1 cut(s) 680
TfiI GAWTC 2 cut(s) 198, 368
Tru1I TTAA 6 cut(s) 224, 446, 480, 728, 801, 1140
Tru9I TTAA 6 cut(s) 224, 446, 480, 728, 801, 1140
TscAI CASTG 1 cut(s) 825
TspDTI ATGAA 4 cut(s) 711, 914, 1023, 1034
TspGWI ACGGA 1 cut(s) 1111
TspRI CASTG 1 cut(s) 825
Van91I CCANNNNNTGG 1 cut(s) 324
XagI CCTNNNNNAGG 1 cut(s) 629
XapI RAATTY 6 cut(s) 13, 25, 473, 698, 995, 1204
XceI RCATGY 1 cut(s) 1079
XmiI GTMKAC 1 cut(s) 219
Zsp2I ATGCAT 1 cut(s) 1153
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.