RchiOBHm_Chr3g0466681

Hydrolyzes acetyl esters in homogalacturonan regions of pectin. In type I primary cell wall, galacturonic acid residues of pectin can be acetylated at the O-2 and O-3 positions. Decreasing the degree of acetylation of pectin gels in vitro alters their physical properties

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
3
OG0001587
Physical Location & Seq
Reverse (-)
13086514 .. 13087580
1067 bp
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UTR
Exon/CDS
Intron
PRQ43273

Sequence Viewer

Length: 246 bp
ATGGAAAATGGATCAAAACTTTTCTTTAGGGGACAGCTCATCTGGGAAGCAATTATGGATGAACTCTTGTCAATTGGCTTGTCAAAGTCAAAACAGGCGCTTCTTTCTGGATGCTCAGCCGGCGGGTTGGCAACTCTTATACACTGTGATGACTTTCGAGGTCTTCTACCTAGGGATTCAAGGATTCAACTGTCAAATGTCATGCCGATGCAGTTGATGGAGCTAATATGGGATGCCTATCAGTAG

Protein Analysis

81

Amino Acids

9.07

Weight (kDa)

5.12

Isoelectric Point (pI)

22.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PAE PF03283 3 - 63 7e-23 Pectinacetylesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 123
AclWI GGATC 1 cut(s) 19
AgsI TTSAA 2 cut(s) 180, 188
AluBI AGCT 2 cut(s) 37, 223
AluI AGCT 2 cut(s) 37, 223
AlwI GGATC 1 cut(s) 19
AspA2I CCTAGG 1 cut(s) 170
AspLEI GCGC 1 cut(s) 100
AvrII CCTAGG 1 cut(s) 170
BbsI GAAGAC 1 cut(s) 155
BccI CCATC 1 cut(s) 211
BfaI CTAG 1 cut(s) 171
BfoI RGCGCY 1 cut(s) 101
BlnI CCTAGG 1 cut(s) 170
BlpI GCTNAGC 1 cut(s) 115
BmsI GCATC 3 cut(s) 101, 198, 223
BpiI GAAGAC 1 cut(s) 155
Bpu1102I GCTNAGC 1 cut(s) 115
BsaBI GATNNNNATC 1 cut(s) 237
BsaJI CCNNGG 1 cut(s) 170
Bse118I RCCGGY 1 cut(s) 119
Bse8I GATNNNNATC 1 cut(s) 237
BseDI CCNNGG 1 cut(s) 170
BseGI GGATG 3 cut(s) 64, 116, 238
BseJI GATNNNNATC 1 cut(s) 237
BseMII CTCAG 1 cut(s) 129
BsiSI CCGG 1 cut(s) 120
BslFI GGGAC 1 cut(s) 45
BsmFI GGGAC 1 cut(s) 45
Bsp143I GATC 1 cut(s) 11
Bsp1720I GCTNAGC 1 cut(s) 115
BspACI CCGC 1 cut(s) 123
BspCNI CTCAG 1 cut(s) 128
BspPI GGATC 1 cut(s) 19
BsrFI RCCGGY 1 cut(s) 119
BssAI RCCGGY 1 cut(s) 119
BssECI CCNNGG 1 cut(s) 170
BssMI GATC 1 cut(s) 11
BssT1I CCWWGG 1 cut(s) 170
Bst4CI ACNGT 2 cut(s) 146, 192
BstC8I GCNNGC 1 cut(s) 121
BstDEI CTNAG 1 cut(s) 115
BstF5I GGATG 3 cut(s) 64, 116, 238
BstH2I RGCGCY 1 cut(s) 101
BstHHI GCGC 1 cut(s) 100
BstKTI GATC 1 cut(s) 14
BstMBI GATC 1 cut(s) 11
BstMWI GCNNNNNNNGC 1 cut(s) 120
BstV2I GAAGAC 1 cut(s) 155
BtsCI GGATG 3 cut(s) 64, 116, 238
BtsIMutI CAGTG 1 cut(s) 142
Cac8I GCNNGC 1 cut(s) 121
CfoI GCGC 1 cut(s) 100
Cfr10I RCCGGY 1 cut(s) 119
CviAII CATG 1 cut(s) 202
CviJI RGCY 4 cut(s) 37, 78, 119, 223
CviKI_1 RGCY 4 cut(s) 37, 78, 119, 223
DdeI CTNAG 1 cut(s) 115
DpnI GATC 1 cut(s) 13
DpnII GATC 1 cut(s) 11
Eco130I CCWWGG 1 cut(s) 170
EcoT14I CCWWGG 1 cut(s) 170
ErhI CCWWGG 1 cut(s) 170
FaeI CATG 1 cut(s) 205
FaiI YATR 4 cut(s) 56, 140, 203, 229
FaqI GGGAC 1 cut(s) 45
FatI CATG 1 cut(s) 201
FauI CCCGC 1 cut(s) 116
FokI GGATG 2 cut(s) 71, 123
FspBI CTAG 1 cut(s) 171
GlaI GCGC 1 cut(s) 99
HaeII RGCGCY 1 cut(s) 101
HapII CCGG 1 cut(s) 120
HhaI GCGC 1 cut(s) 100
Hin1II CATG 1 cut(s) 205
Hin6I GCGC 1 cut(s) 98
HinP1I GCGC 1 cut(s) 98
HinfI GANTC 2 cut(s) 176, 184
HpaII CCGG 1 cut(s) 120
Hpy188III TCNNGA 1 cut(s) 108
HpyCH4III ACNGT 2 cut(s) 146, 192
HpyCH4V TGCA 1 cut(s) 211
HpyF10VI GCNNNNNNNGC 1 cut(s) 120
HpyF3I CTNAG 1 cut(s) 115
Hsp92II CATG 1 cut(s) 205
HspAI GCGC 1 cut(s) 98
KroI GCCGGC 1 cut(s) 119
KroNI GCCGGC 1 cut(s) 121
Kzo9I GATC 1 cut(s) 11
LmnI GCTCC 1 cut(s) 220
LpnPI CCDG 4 cut(s) 28, 80, 93, 133
LweI GCATC 3 cut(s) 101, 198, 223
MaeI CTAG 1 cut(s) 171
MalI GATC 1 cut(s) 13
MboI GATC 1 cut(s) 11
MboII GAAGA 1 cut(s) 155
MfeI CAATTG 1 cut(s) 72
MluCI AATT 2 cut(s) 51, 72
MnlI CCTC 1 cut(s) 152
MroNI GCCGGC 1 cut(s) 119
MslI CAYNNNNRTG 2 cut(s) 147, 206
MspI CCGG 1 cut(s) 120
MunI CAATTG 1 cut(s) 72
MwoI GCNNNNNNNGC 1 cut(s) 120
NaeI GCCGGC 1 cut(s) 121
NdeII GATC 1 cut(s) 11
NgoMIV GCCGGC 1 cut(s) 119
NlaIII CATG 1 cut(s) 205
PdiI GCCGGC 1 cut(s) 121
PfeI GAWTC 2 cut(s) 176, 184
RseI CAYNNNNRTG 2 cut(s) 147, 206
Sau3AI GATC 1 cut(s) 11
SetI ASST 4 cut(s) 39, 163, 172, 225
SfaNI GCATC 3 cut(s) 101, 198, 223
SmiMI CAYNNNNRTG 2 cut(s) 147, 206
Sse9I AATT 2 cut(s) 51, 72
SsiI CCGC 1 cut(s) 123
SspMI CTAG 1 cut(s) 171
StyI CCWWGG 1 cut(s) 170
TaaI ACNGT 2 cut(s) 146, 192
TaqI TCGA 1 cut(s) 157
TasI AATT 2 cut(s) 51, 72
TfiI GAWTC 2 cut(s) 176, 184
TscAI CASTG 1 cut(s) 149
TspDTI ATGAA 1 cut(s) 75
TspRI CASTG 1 cut(s) 149
XmaJI CCTAGG 1 cut(s) 170
XspI CTAG 1 cut(s) 171
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.