FvH4_6g13810

Hydrolyzes acetyl esters in homogalacturonan regions of pectin. In type I primary cell wall, galacturonic acid residues of pectin can be acetylated at the O-2 and O-3 positions. Decreasing the degree of acetylation of pectin gels in vitro alters their physical properties

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0001587
Physical Location & Seq
Reverse (-)
8432138 .. 8436347
4210 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g13810.t8

Sequence Viewer

Length: 1308 bp
ATGGCGGGGTCAACGGCGAACCTCAGACTCCGCAGCAGCCTTGTGTGGTGGAGAAAGTGGGCCAGGAAGGACTGGGCAATCGCCACCGTGGCCTTCACCGTCATTCTCTTCCTCTTTCTCACTCTCATCTCCAACTCCTCTGACTCCGGCGTCACCAACCCCTCCCATCCAATCACCTCCGCCGATTTAGTTGACCTGACTCTGCTGCACGACGCCGAACGAAGAGGCGCCCTTTGCTTAGATGGGAGTGCTCCGGGATATCATTTCCGAAAGGGATTTGGATCCGGAGCGGAGAATTGGCTGCTTCACATTGAGGGTGGAGGTTGGTGCAATACAATGAAATCATGTTCTTTGCGGAAATGGACTCCGTTAGGGTCTTCCAAGTACATGGATCGTAGAGTTCCGTTTTCAGGGATTTTGAGCTCTCATTCTTCAGAAAATCCTGACTTCTTTAACTGGAACAAAGTGAAGATACGGTACTGTGATGGCGCTTCTTTTGCTGGCCACCCGGCAAATGAGCCCAAGAATGGATCAAAGCTTTTCTTTAGGGGACAGCTTATCTGGGAAGCACTGATGGATGAATTCTCGTCAATTGGCTTGTCAAAGGCGAAACAGGCGCTTCTTTCTGGATGCTCTGCTGGTGGGTTGGCAACTCTTATACACTGTGATGACTTTCGAGGTCTTCTACCTAGGGATTCAACTGTCAAGTGTCTTGCTGATGCAGGTTTTTTTCTTGATGAGAAAGATGTTAACCAAAACCGGACCATGAGAAATTTTTATCGTGATGTTGTGGTCCTTCAGGATGTAGCAAAAAGTTTGCACAAGGATTGTGTTGCAAAGAAGGAACCAGCTAAGTGCCTCTTTCCTGAAGAAATTATTAAACACGTTAGCACTCCTCTGTTCCTTGTCAACCCAGCTTATGATTTTTGGCAGATTCAACATATCTTGATACCTGAAGCATCAGACCCGTATGGCTACTGGAAAAGGTGCAAGCTGAACATCCATAATTGCAATCCTAGCCAGATTGAGATACTTCAAGGTTTCCGTCGTTCTATGCTAAATGCACTGAGCGAATTCCAGAAAAATAAGGATGGAGGAATGTTTATAAATTCATGTTTTATTCATTGCCAGACATGGTTTACCGAGACATGGCATTCACCCAGTTCTCCGCGAATAAACAATAAGACAATTGCAGAGTCTGTGGGTGATTGGTACTTTAATAGAAATGTTGCAAAGCAAATTGACTGCCCATTTCCATGCAATCCCACCTGTTATCATATGAATTTCACTCAATTTTCACGAGGTTGA

Protein Analysis

436

Amino Acids

49.16

Weight (kDa)

8.78

Isoelectric Point (pI)

47.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PAE PF03283 61 - 290 1.7e-99 Pectinacetylesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1106
AasI GACNNNNNNGTC 1 cut(s) 149
Acc36I ACCTGC 1 cut(s) 713
AccB1I GGYRCC 1 cut(s) 227
AccBSI CCGCTC 1 cut(s) 290
AccII CGCG 1 cut(s) 1171
AccIII TCCGGA 1 cut(s) 284
AciI CCGC 6 cut(s) 5, 31, 180, 290, 355, 1169
AclWI GGATC 4 cut(s) 276, 289, 399, 538
AcoI YGGCCR 1 cut(s) 502
AcsI RAATTY 5 cut(s) 581, 772, 1073, 1108, 1282
AcuI CTGAAG 4 cut(s) 417, 782, 888, 975
AcyI GRCGYC 3 cut(s) 150, 213, 228
AfaI GTAC 3 cut(s) 386, 479, 1214
AfiI CCNNNNNNNGG 3 cut(s) 410, 527, 1149
AflIII ACRYGT 1 cut(s) 883
AgsI TTSAA 3 cut(s) 699, 938, 1037
AjnI CCWGG 1 cut(s) 62
AluBI AGCT 6 cut(s) 423, 538, 556, 851, 917, 994
AluI AGCT 6 cut(s) 423, 538, 556, 851, 917, 994
Alw21I GWGCWC 2 cut(s) 253, 425
Alw26I GTCTC 1 cut(s) 1139
AlwI GGATC 4 cut(s) 276, 289, 399, 538
AlwNI CAGNNNCTG 1 cut(s) 1199
Aor13HI TCCGGA 1 cut(s) 284
AoxI GGCC 3 cut(s) 60, 90, 502
ApeKI GCWGC 4 cut(s) 33, 36, 205, 301
ApoI RAATTY 5 cut(s) 581, 772, 1073, 1108, 1282
AspA2I CCTAGG 1 cut(s) 689
AspLEI GCGC 3 cut(s) 230, 491, 619
AspS9I GGNCC 3 cut(s) 60, 762, 793
AsuC2I CCSGG 2 cut(s) 255, 509
AsuHPI GGTGA 5 cut(s) 88, 145, 166, 1149, 1217
AvaII GGWCC 2 cut(s) 762, 793
AvrII CCTAGG 1 cut(s) 689
BalI TGGCCA 1 cut(s) 504
BamHI GGATCC 1 cut(s) 281
BanI GGYRCC 1 cut(s) 227
BanII GRGCYC 2 cut(s) 425, 522
BarI GAAGNNNNNNTAC 2 cut(s) 461, 493
BauI CACGAG 1 cut(s) 1299
BbsI GAAGAC 2 cut(s) 369, 674
Bbv12I GWGCWC 2 cut(s) 253, 425
BbvI GCAGC 4 cut(s) 45, 48, 192, 288
BccI CCATC 5 cut(s) 174, 236, 479, 568, 1085
BceAI ACGGC 1 cut(s) 30
BciT130I CCWGG 1 cut(s) 64
BcnI CCSGG 2 cut(s) 255, 509
BcoDI GTCTC 1 cut(s) 1139
BfaI CTAG 2 cut(s) 690, 1017
BfoI RGCGCY 3 cut(s) 231, 492, 620
BfuAI ACCTGC 1 cut(s) 713
BglI GCCNNNNNGGC 1 cut(s) 89
BisI GCNGC 4 cut(s) 34, 37, 206, 302
BlnI CCTAGG 1 cut(s) 689
BlsI GCNGC 4 cut(s) 35, 38, 207, 303
Bme1390I CCNGG 3 cut(s) 64, 255, 509
Bme18I GGWCC 2 cut(s) 762, 793
BmgT120I GGNCC 3 cut(s) 60, 762, 793
BmiI GGNNCC 3 cut(s) 229, 283, 846
BmrFI CCNGG 3 cut(s) 64, 255, 509
BmrI ACTGGG 2 cut(s) 82, 1155
BmsI GCATC 3 cut(s) 620, 709, 968
BmuI ACTGGG 2 cut(s) 82, 1155
BpiI GAAGAC 2 cut(s) 369, 674
BpuMI CCSGG 2 cut(s) 255, 509
BsaBI GATNNNNATC 1 cut(s) 280
BsaHI GRCGYC 3 cut(s) 150, 213, 228
BsaJI CCNNGG 2 cut(s) 87, 689
BsaWI WCCGGW 2 cut(s) 284, 759
Bsc4I CCNNNNNNNGG 3 cut(s) 410, 527, 1149
Bse1I ACTGG 4 cut(s) 77, 461, 983, 1161
Bse3DI GCAATG 1 cut(s) 1123
Bse8I GATNNNNATC 1 cut(s) 280
BseAI TCCGGA 1 cut(s) 284
BseBI CCWGG 1 cut(s) 64
BseDI CCNNGG 2 cut(s) 87, 689
BseGI GGATG 6 cut(s) 166, 583, 635, 808, 999, 1096
BseJI GATNNNNATC 1 cut(s) 280
BseLI CCNNNNNNNGG 3 cut(s) 410, 527, 1149
BseMI GCAATG 1 cut(s) 1123
BseMII CTCAG 2 cut(s) 37, 1058
BseNI ACTGG 4 cut(s) 77, 461, 983, 1161
BseRI GAGGAG 2 cut(s) 127, 885
BseXI GCAGC 4 cut(s) 45, 48, 192, 288
BseYI CCCAGC 1 cut(s) 913
BsgI GTGCAG 1 cut(s) 191
Bsh1236I CGCG 1 cut(s) 1171
BshFI GGCC 3 cut(s) 62, 92, 504
BshNI GGYRCC 1 cut(s) 227
BsiHKAI GWGCWC 2 cut(s) 253, 425
BsiSI CCGG 5 cut(s) 147, 254, 285, 509, 760
BslFI GGGAC 1 cut(s) 564
BslI CCNNNNNNNGG 3 cut(s) 410, 527, 1149
BsmAI GTCTC 1 cut(s) 1139
BsmFI GGGAC 1 cut(s) 564
BsmI GAATGC 1 cut(s) 1153
BsnI GGCC 3 cut(s) 62, 92, 504
Bsp1286I GDGCHC 3 cut(s) 253, 425, 522
Bsp13I TCCGGA 1 cut(s) 284
Bsp143I GATC 3 cut(s) 281, 391, 530
BspACI CCGC 6 cut(s) 5, 31, 180, 290, 355, 1169
BspANI GGCC 3 cut(s) 62, 92, 504
BspCNI CTCAG 2 cut(s) 36, 1059
BspEI TCCGGA 1 cut(s) 284
BspFNI CGCG 1 cut(s) 1171
BspLI GGNNCC 3 cut(s) 229, 283, 846
BspMI ACCTGC 1 cut(s) 713
BspPI GGATC 4 cut(s) 276, 289, 399, 538
BspT107I GGYRCC 1 cut(s) 227
BsrBI CCGCTC 1 cut(s) 290
BsrDI GCAATG 1 cut(s) 1123
BsrI ACTGG 4 cut(s) 77, 461, 983, 1161
BssECI CCNNGG 2 cut(s) 87, 689
BssMI GATC 3 cut(s) 281, 391, 530
BssNI GRCGYC 3 cut(s) 150, 213, 228
BssSI CACGAG 1 cut(s) 1299
BssT1I CCWWGG 1 cut(s) 689
Bst2BI CACGAG 1 cut(s) 1299
Bst2UI CCWGG 1 cut(s) 64
Bst4CI ACNGT 6 cut(s) 88, 100, 477, 482, 665, 703
Bst6I CTCTTC 2 cut(s) 113, 217
BstACI GRCGYC 3 cut(s) 150, 213, 228
BstC8I GCNNGC 2 cut(s) 502, 992
BstDEI CTNAG 4 cut(s) 23, 238, 852, 1067
BstDSI CCRYGG 1 cut(s) 87
BstF5I GGATG 6 cut(s) 166, 583, 635, 808, 999, 1096
BstFNI CGCG 1 cut(s) 1171
BstH2I RGCGCY 3 cut(s) 231, 492, 620
BstHHI GCGC 3 cut(s) 230, 491, 619
BstKTI GATC 3 cut(s) 284, 394, 533
BstMAI GTCTC 1 cut(s) 1139
BstMBI GATC 3 cut(s) 281, 391, 530
BstMWI GCNNNNNNNGC 5 cut(s) 89, 234, 497, 614, 1017
BstNI CCWGG 1 cut(s) 64
BstSCI CCNGG 3 cut(s) 62, 253, 507
BstUI CGCG 1 cut(s) 1171
BstV1I GCAGC 4 cut(s) 45, 48, 192, 288
BstV2I GAAGAC 2 cut(s) 369, 674
BstX2I RGATCY 1 cut(s) 281
BstXI CCANNNNNNTGG 1 cut(s) 388
BstYI RGATCY 1 cut(s) 281
BsuRI GGCC 3 cut(s) 62, 92, 504
BtgI CCRYGG 1 cut(s) 87
BtsCI GGATG 6 cut(s) 166, 583, 635, 808, 999, 1096
BtsIMutI CAGTG 3 cut(s) 569, 661, 1064
BveI ACCTGC 1 cut(s) 713
Cac8I GCNNGC 2 cut(s) 502, 992
CaiI CAGNNNCTG 1 cut(s) 1199
CfoI GCGC 3 cut(s) 230, 491, 619
Cfr13I GGNCC 3 cut(s) 60, 762, 793
CseI GACGC 2 cut(s) 139, 221
Csp6I GTAC 3 cut(s) 385, 478, 1213
CviAII CATG 7 cut(s) 345, 388, 766, 1113, 1134, 1149, 1257
CviQI GTAC 3 cut(s) 385, 478, 1213
DdeI CTNAG 4 cut(s) 23, 238, 852, 1067
DinI GGCGCC 1 cut(s) 229
DpnI GATC 3 cut(s) 283, 393, 532
DpnII GATC 3 cut(s) 281, 391, 530
DrdI GACNNNNNNGTC 1 cut(s) 149
DseDI GACNNNNNNGTC 1 cut(s) 149
EaeI YGGCCR 1 cut(s) 502
Eam1104I CTCTTC 2 cut(s) 113, 217
EarI CTCTTC 2 cut(s) 113, 217
EciI GGCGGA 1 cut(s) 169
Ecl136II GAGCTC 1 cut(s) 423
Eco130I CCWWGG 1 cut(s) 689
Eco24I GRGCYC 2 cut(s) 425, 522
Eco32I GATATC 1 cut(s) 260
Eco47I GGWCC 2 cut(s) 762, 793
Eco53kI GAGCTC 1 cut(s) 423
Eco57I CTGAAG 4 cut(s) 417, 782, 888, 975
EcoICRI GAGCTC 1 cut(s) 423
EcoRI GAATTC 2 cut(s) 581, 1073
EcoRII CCWGG 1 cut(s) 62
EcoRV GATATC 1 cut(s) 260
EcoT14I CCWWGG 1 cut(s) 689
EcoT38I GRGCYC 2 cut(s) 425, 522
EgeI GGCGCC 1 cut(s) 229
EheI GGCGCC 1 cut(s) 229
ErhI CCWWGG 1 cut(s) 689
FaeI CATG 7 cut(s) 348, 391, 769, 1116, 1137, 1152, 1260
FalI AAGNNNNNCTT 4 cut(s) 527, 559, 845, 877
FaqI GGGAC 1 cut(s) 564
FatI CATG 7 cut(s) 344, 387, 765, 1112, 1133, 1148, 1256
FauNDI CATATG 1 cut(s) 1278
Fnu4HI GCNGC 4 cut(s) 34, 37, 206, 302
FokI GGATG 6 cut(s) 153, 590, 642, 815, 986, 1103
FriOI GRGCYC 2 cut(s) 425, 522
Fsp4HI GCNGC 4 cut(s) 34, 37, 206, 302
FspBI CTAG 2 cut(s) 690, 1017
GlaI GCGC 3 cut(s) 229, 490, 618
GluI GCNGC 4 cut(s) 34, 37, 206, 302
GsaI CCCAGC 1 cut(s) 917
HaeII RGCGCY 3 cut(s) 231, 492, 620
HaeIII GGCC 3 cut(s) 62, 92, 504
HapII CCGG 5 cut(s) 147, 254, 285, 509, 760
HgaI GACGC 2 cut(s) 139, 221
HhaI GCGC 3 cut(s) 230, 491, 619
Hin1I GRCGYC 3 cut(s) 150, 213, 228
Hin1II CATG 7 cut(s) 348, 391, 769, 1116, 1137, 1152, 1260
Hin6I GCGC 3 cut(s) 228, 489, 617
HinP1I GCGC 3 cut(s) 228, 489, 617
HincII GTYRAC 4 cut(s) 12, 193, 751, 910
HindII GTYRAC 4 cut(s) 12, 193, 751, 910
HindIII AAGCTT 1 cut(s) 536
HinfI GANTC 7 cut(s) 27, 143, 199, 364, 695, 934, 1196
HpaI GTTAAC 1 cut(s) 751
HpaII CCGG 5 cut(s) 147, 254, 285, 509, 760
HphI GGTGA 5 cut(s) 88, 145, 166, 1149, 1217
Hpy166II GTNNAC 5 cut(s) 12, 193, 751, 910, 1140
Hpy188I TCNGA 5 cut(s) 26, 142, 269, 436, 964
Hpy8I GTNNAC 5 cut(s) 12, 193, 751, 910, 1140
Hpy99I CGWCG 2 cut(s) 215, 1050
HpyAV CCTTC 4 cut(s) 61, 103, 806, 835
HpyCH4III ACNGT 6 cut(s) 88, 100, 477, 482, 665, 703
HpyCH4IV ACGT 1 cut(s) 885
HpyF10VI GCNNNNNNNGC 5 cut(s) 89, 234, 497, 614, 1017
HpyF3I CTNAG 4 cut(s) 23, 238, 852, 1067
HpySE526I ACGT 1 cut(s) 885
Hsp92I GRCGYC 3 cut(s) 150, 213, 228
Hsp92II CATG 7 cut(s) 348, 391, 769, 1116, 1137, 1152, 1260
HspAI GCGC 3 cut(s) 228, 489, 617
KasI GGCGCC 1 cut(s) 227
Kpn2I TCCGGA 1 cut(s) 284
KspAI GTTAAC 1 cut(s) 751
Kzo9I GATC 3 cut(s) 281, 391, 530
LmnI GCTCC 2 cut(s) 256, 287
Lsp1109I GCAGC 4 cut(s) 45, 48, 192, 288
LweI GCATC 3 cut(s) 620, 709, 968
MaeI CTAG 2 cut(s) 690, 1017
MaeII ACGT 1 cut(s) 885
MaeIII GTNAC 1 cut(s) 151
MalI GATC 3 cut(s) 283, 393, 532
MbiI CCGCTC 1 cut(s) 290
MboI GATC 3 cut(s) 281, 391, 530
MboII GAAGA 7 cut(s) 100, 234, 369, 423, 481, 674, 881
MfeI CAATTG 2 cut(s) 591, 1188
MflI RGATCY 1 cut(s) 281
MhlI GDGCHC 3 cut(s) 253, 425, 522
MlsI TGGCCA 1 cut(s) 504
MluNI TGGCCA 1 cut(s) 504
Mly113I GGCGCC 1 cut(s) 228
MlyI GAGTC 5 cut(s) 21, 137, 193, 358, 1205
MmeI TCCRAC 1 cut(s) 156
Mox20I TGGCCA 1 cut(s) 504
MroI TCCGGA 1 cut(s) 284
MscI TGGCCA 1 cut(s) 504
MseI TTAA 4 cut(s) 453, 750, 879, 1218
MslI CAYNNNNRTG 2 cut(s) 666, 1255
Msp20I TGGCCA 1 cut(s) 504
MspI CCGG 5 cut(s) 147, 254, 285, 509, 760
MspR9I CCNGG 3 cut(s) 64, 255, 509
MunI CAATTG 2 cut(s) 591, 1188
Mva1269I GAATGC 1 cut(s) 1153
MvaI CCWGG 1 cut(s) 64
MvnI CGCG 1 cut(s) 1171
MwoI GCNNNNNNNGC 5 cut(s) 89, 234, 497, 614, 1017
NarI GGCGCC 1 cut(s) 228
NciI CCSGG 2 cut(s) 255, 509
NdeI CATATG 1 cut(s) 1278
NdeII GATC 3 cut(s) 281, 391, 530
NlaIII CATG 7 cut(s) 348, 391, 769, 1116, 1137, 1152, 1260
NlaIV GGNNCC 3 cut(s) 229, 283, 846
NmuCI GTSAC 1 cut(s) 151
PctI GAATGC 1 cut(s) 1153
PfeI GAWTC 2 cut(s) 695, 934
PfoI TCCNGGA 1 cut(s) 253
PkrI GCNGC 4 cut(s) 35, 38, 207, 303
PleI GAGTC 5 cut(s) 21, 137, 193, 358, 1204
PluTI GGCGCC 1 cut(s) 231
PpsI GAGTC 5 cut(s) 21, 137, 193, 358, 1204
PsiI TTATAA 1 cut(s) 1106
Psp124BI GAGCTC 1 cut(s) 425
Psp6I CCWGG 1 cut(s) 62
PspFI CCCAGC 1 cut(s) 913
PspGI CCWGG 1 cut(s) 62
PspN4I GGNNCC 3 cut(s) 229, 283, 846
PspPI GGNCC 3 cut(s) 60, 762, 793
PstNI CAGNNNCTG 1 cut(s) 1199
PsuI RGATCY 1 cut(s) 281
RsaI GTAC 3 cut(s) 386, 479, 1214
RsaNI GTAC 3 cut(s) 385, 478, 1213
RseI CAYNNNNRTG 2 cut(s) 666, 1255
SacI GAGCTC 1 cut(s) 425
SaqAI TTAA 4 cut(s) 453, 750, 879, 1218
SatI GCNGC 4 cut(s) 34, 37, 206, 302
Sau3AI GATC 3 cut(s) 281, 391, 530
Sau96I GGNCC 3 cut(s) 60, 762, 793
SchI GAGTC 5 cut(s) 21, 137, 193, 358, 1205
ScrFI CCNGG 3 cut(s) 64, 255, 509
SduI GDGCHC 3 cut(s) 253, 425, 522
SfaNI GCATC 3 cut(s) 620, 709, 968
SfoI GGCGCC 1 cut(s) 229
SinI GGWCC 2 cut(s) 762, 793
SmiMI CAYNNNNRTG 2 cut(s) 666, 1255
SsiI CCGC 6 cut(s) 5, 31, 180, 290, 355, 1169
SspDI GGCGCC 1 cut(s) 227
SspMI CTAG 2 cut(s) 690, 1017
SstI GAGCTC 1 cut(s) 425
StyD4I CCNGG 3 cut(s) 62, 253, 507
StyI CCWWGG 1 cut(s) 689
TaaI ACNGT 6 cut(s) 88, 100, 477, 482, 665, 703
TaiI ACGT 1 cut(s) 888
TaqI TCGA 1 cut(s) 676
TatI WGTACW 1 cut(s) 384
TfiI GAWTC 2 cut(s) 695, 934
Tru1I TTAA 4 cut(s) 453, 750, 879, 1218
Tru9I TTAA 4 cut(s) 453, 750, 879, 1218
TscAI CASTG 3 cut(s) 576, 668, 1071
TseFI GTSAC 1 cut(s) 151
TseI GCWGC 4 cut(s) 33, 36, 205, 301
Tsp45I GTSAC 1 cut(s) 151
TspDTI ATGAA 5 cut(s) 353, 594, 1101, 1112, 1295
TspGWI ACGGA 3 cut(s) 357, 393, 1034
TspRI CASTG 3 cut(s) 576, 668, 1071
VpaK11BI GGWCC 2 cut(s) 762, 793
XapI RAATTY 5 cut(s) 581, 772, 1073, 1108, 1282
XmaJI CCTAGG 1 cut(s) 689
XspI CTAG 2 cut(s) 690, 1017
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.