pycom12g13590

Hydrolyzes acetyl esters in homogalacturonan regions of pectin. In type I primary cell wall, galacturonic acid residues of pectin can be acetylated at the O-2 and O-3 positions. Decreasing the degree of acetylation of pectin gels in vitro alters their physical properties

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0001587
Physical Location & Seq
Forward (+)
16074683 .. 16078316
3634 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom12g13590.9

Sequence Viewer

Length: 1185 bp
ATGGTGGGGTCAATGGCGAATCCCAGGCTTCGCAGCCTGCCATGGTGGCGGAGGTGGACCAAAAAGGACTGGGCAATCGCCGCCGTCGGATTTACCGTTATCGCCTTCGCTCTCACTCTCCTCTCCAGCTCTAGGCGCGACAAGCTAGACTCCGGAATCTCCAGCCCGTTTCTTCCCAACGCCGCCGCCTCCGACTTGGTCGAGTTGACCTTGCTCAAAAACGCTAAGGATAGAGGCGCCCTTTGCTTGGATGGGACTGCCCCTGGCTACCATTTCAGGAAGGGTTTCGGATCCGGCGCCAACAATTGGTTGCTTCACATTGAGGGTGGAGGTTGGTGCAATTCGACTGAATCGTGTTCTTGGCGCAAAGGTACTCCATTAGGGTCTTCTAAGTACATGGATCGTCGAGTTTCCTTTTCCGGGATTTTGAGCTCTCATCCATCGCAAAATCCTGAGTTCTTTAACTGGAATAAAGTCAGGATCCGATACTGTGATGGGGCGTCCTTGGCTGGCCACCCGGAAAATGAGTCTAAGAATGGATCGGAACTTTTCTTTAGGGGCCAACTCATCTGGGAAGCAGTTATCGATGAACTCTTGTCAATTGGCATGTCAAAAGTGAAACAGGCCCTTCTTTCAGGATGCTCTGCCGGTGGGTTAGCAACTCTTATACATTGTGATGACTTTCGAGGTCTTCTGCCTAAAGATGCAACTGTCAAATGTCTTGCTGATGGAGGTTTTTTCCTTGATGAGGAAGATATTCTCCGGCATCGGACTATGAGGTCTTTATATAACGATGTTGCCATCCTTCAGGGTTTAGCAAAAAGTTTGCACAAGGATTGTATTTCGAAGATGGAACCAGCTAAGTGTCTTTTTCCTGAAGAAATTATTAAACACAGTAACACCCCACTGTTCCTTGTCAACTCAGCTTACGATTTTTGGCAGGTACAACATGTCTTGATACCTGAAGCAGCAGATCCACATGGCTACTGGAAAAAGTGCAAACTGAACATTCACAATTGCAATTCCAACCAGCTTGAAATTCTACAAGAGCGTGCTCCTGTTCCTGTAGACATTGAGAAGGAAGGTGTTGATCAGACCCGGAAAGAAAATAGAGACAAGGTGTCGAGAAACAGGAAAAACAGTTTATACTTGTCATCGTGTTACAAGTTCGATGTTCTCTTGTAA

Protein Analysis

395

Amino Acids

44.11

Weight (kDa)

8.73

Isoelectric Point (pI)

49.84

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 778
Acc36I ACCTGC 1 cut(s) 931
AccB1I GGYRCC 2 cut(s) 236, 296
AccB7I CCANNNNNTGG 1 cut(s) 306
AccI GTMKAC 1 cut(s) 1068
AccII CGCG 1 cut(s) 138
AccIII TCCGGA 1 cut(s) 152
AciI CCGC 4 cut(s) 49, 81, 183, 186
AclWI GGATC 7 cut(s) 285, 298, 408, 475, 488, 547, 968
AcoI YGGCCR 1 cut(s) 511
AcsI RAATTY 1 cut(s) 1038
AcuI CTGAAG 3 cut(s) 791, 897, 984
AcyI GRCGYC 3 cut(s) 237, 297, 500
AfaI GTAC 3 cut(s) 373, 395, 945
AfiI CCNNNNNNNGG 5 cut(s) 132, 247, 306, 420, 748
AflIII ACRYGT 1 cut(s) 949
AgsI TTSAA 1 cut(s) 1037
AhdI GACNNNNNGTC 1 cut(s) 1120
AjnI CCWGG 2 cut(s) 23, 262
AluBI AGCT 6 cut(s) 129, 145, 432, 860, 926, 1033
AluI AGCT 6 cut(s) 129, 145, 432, 860, 926, 1033
Alw21I GWGCWC 2 cut(s) 434, 1057
Alw26I GTCTC 1 cut(s) 1107
AlwI GGATC 7 cut(s) 285, 298, 408, 475, 488, 547, 968
Aor13HI TCCGGA 1 cut(s) 152
AoxI GGCC 3 cut(s) 511, 559, 624
ApeKI GCWGC 2 cut(s) 33, 968
ApoI RAATTY 1 cut(s) 1038
Asp700I GAANNNNTTC 2 cut(s) 284, 756
AspLEI GCGC 4 cut(s) 138, 239, 299, 366
AspS9I GGNCC 3 cut(s) 57, 559, 625
AsuC2I CCSGG 3 cut(s) 421, 518, 1099
AsuII TTCGAA 1 cut(s) 845
AvaII GGWCC 1 cut(s) 57
BalI TGGCCA 1 cut(s) 513
BamHI GGATCC 2 cut(s) 290, 480
BanI GGYRCC 2 cut(s) 236, 296
BanII GRGCYC 1 cut(s) 434
BbsI GAAGAC 2 cut(s) 378, 683
Bbv12I GWGCWC 2 cut(s) 434, 1057
BbvI GCAGC 2 cut(s) 45, 980
BccI CCATC 6 cut(s) 245, 448, 488, 722, 809, 844
BceAI ACGGC 1 cut(s) 68
BciT130I CCWGG 2 cut(s) 25, 264
BclI TGATCA 1 cut(s) 1090
BcnI CCSGG 3 cut(s) 421, 518, 1099
BcoDI GTCTC 1 cut(s) 1107
BfaI CTAG 2 cut(s) 132, 146
BfmI CTRYAG 1 cut(s) 1065
BfoI RGCGCY 2 cut(s) 240, 300
BfuAI ACCTGC 1 cut(s) 931
BglI GCCNNNNNGGC 1 cut(s) 46
BisI GCNGC 5 cut(s) 34, 81, 183, 186, 969
BlsI GCNGC 5 cut(s) 35, 82, 184, 187, 970
Bme1390I CCNGG 5 cut(s) 25, 264, 421, 518, 1099
Bme18I GGWCC 1 cut(s) 57
BmeRI GACNNNNNGTC 1 cut(s) 1120
BmgT120I GGNCC 3 cut(s) 57, 559, 625
BmiI GGNNCC 6 cut(s) 238, 292, 298, 482, 560, 855
BmrFI CCNGG 5 cut(s) 25, 264, 421, 518, 1099
BmrI ACTGGG 1 cut(s) 79
BmsI GCATC 3 cut(s) 629, 694, 775
BmuI ACTGGG 1 cut(s) 79
BpiI GAAGAC 2 cut(s) 378, 683
BpmI CTGGAG 2 cut(s) 109, 145
Bpu10I CCTNAGC 1 cut(s) 225
Bpu14I TTCGAA 1 cut(s) 845
BpuMI CCSGG 3 cut(s) 421, 518, 1099
Bsa29I ATCGAT 1 cut(s) 585
BsaHI GRCGYC 3 cut(s) 237, 297, 500
BsaJI CCNNGG 4 cut(s) 23, 41, 262, 504
BsaWI WCCGGW 1 cut(s) 152
Bsc4I CCNNNNNNNGG 5 cut(s) 132, 247, 306, 420, 748
Bse118I RCCGGY 1 cut(s) 647
Bse1I ACTGG 3 cut(s) 74, 470, 992
BseAI TCCGGA 1 cut(s) 152
BseBI CCWGG 2 cut(s) 25, 264
BseCI ATCGAT 1 cut(s) 585
BseDI CCNNGG 4 cut(s) 23, 41, 262, 504
BseGI GGATG 4 cut(s) 256, 436, 644, 801
BseLI CCNNNNNNNGG 5 cut(s) 132, 247, 306, 420, 748
BseMII CTCAG 2 cut(s) 444, 936
BseNI ACTGG 3 cut(s) 74, 470, 992
BseRI GAGGAG 1 cut(s) 110
BseXI GCAGC 2 cut(s) 45, 980
Bsh1236I CGCG 1 cut(s) 138
BshFI GGCC 3 cut(s) 513, 561, 626
BshNI GGYRCC 2 cut(s) 236, 296
BshVI ATCGAT 1 cut(s) 585
BsiHKAI GWGCWC 2 cut(s) 434, 1057
BsiSI CCGG 7 cut(s) 153, 294, 420, 518, 648, 763, 1099
BslFI GGGAC 1 cut(s) 268
BslI CCNNNNNNNGG 5 cut(s) 132, 247, 306, 420, 748
BsmAI GTCTC 1 cut(s) 1107
BsmFI GGGAC 1 cut(s) 268
BsnI GGCC 3 cut(s) 513, 561, 626
Bsp119I TTCGAA 1 cut(s) 845
Bsp1286I GDGCHC 2 cut(s) 434, 1057
Bsp13I TCCGGA 1 cut(s) 152
Bsp143I GATC 6 cut(s) 290, 400, 480, 539, 973, 1090
Bsp19I CCATGG 1 cut(s) 41
BspACI CCGC 4 cut(s) 49, 81, 183, 186
BspANI GGCC 3 cut(s) 513, 561, 626
BspCNI CTCAG 2 cut(s) 445, 935
BspDI ATCGAT 1 cut(s) 585
BspEI TCCGGA 1 cut(s) 152
BspFNI CGCG 1 cut(s) 138
BspLI GGNNCC 6 cut(s) 238, 292, 298, 482, 560, 855
BspMI ACCTGC 1 cut(s) 931
BspPI GGATC 7 cut(s) 285, 298, 408, 475, 488, 547, 968
BspT104I TTCGAA 1 cut(s) 845
BspT107I GGYRCC 2 cut(s) 236, 296
BsrFI RCCGGY 1 cut(s) 647
BsrI ACTGG 3 cut(s) 74, 470, 992
BssAI RCCGGY 1 cut(s) 647
BssECI CCNNGG 4 cut(s) 23, 41, 262, 504
BssMI GATC 6 cut(s) 290, 400, 480, 539, 973, 1090
BssNI GRCGYC 3 cut(s) 237, 297, 500
BssT1I CCWWGG 2 cut(s) 41, 504
Bst2UI CCWGG 2 cut(s) 25, 264
Bst4CI ACNGT 6 cut(s) 97, 491, 712, 896, 909, 1142
BstACI GRCGYC 3 cut(s) 237, 297, 500
BstBI TTCGAA 1 cut(s) 845
BstC8I GCNNGC 3 cut(s) 38, 511, 1053
BstDEI CTNAG 6 cut(s) 225, 390, 453, 531, 861, 922
BstDSI CCRYGG 1 cut(s) 41
BstENI CCTNNNNNAGG 1 cut(s) 746
BstF5I GGATG 4 cut(s) 256, 436, 644, 801
BstFNI CGCG 1 cut(s) 138
BstH2I RGCGCY 2 cut(s) 240, 300
BstHHI GCGC 4 cut(s) 138, 239, 299, 366
BstKTI GATC 6 cut(s) 293, 403, 483, 542, 976, 1093
BstMAI GTCTC 1 cut(s) 1107
BstMBI GATC 6 cut(s) 290, 400, 480, 539, 973, 1090
BstMWI GCNNNNNNNGC 6 cut(s) 46, 80, 135, 142, 243, 506
BstNI CCWGG 2 cut(s) 25, 264
BstNSI RCATGY 2 cut(s) 610, 953
BstSCI CCNGG 5 cut(s) 23, 262, 419, 516, 1097
BstSFI CTRYAG 1 cut(s) 1065
BstUI CGCG 1 cut(s) 138
BstV1I GCAGC 2 cut(s) 45, 980
BstV2I GAAGAC 2 cut(s) 378, 683
BstX2I RGATCY 3 cut(s) 290, 480, 973
BstYI RGATCY 3 cut(s) 290, 480, 973
Bsu15I ATCGAT 1 cut(s) 585
BsuRI GGCC 3 cut(s) 513, 561, 626
BsuTUI ATCGAT 1 cut(s) 585
BtgI CCRYGG 1 cut(s) 41
BtgZI GCGATG 1 cut(s) 426
BtsCI GGATG 4 cut(s) 256, 436, 644, 801
BtsIMutI CAGTG 1 cut(s) 905
BveI ACCTGC 1 cut(s) 931
Cac8I GCNNGC 3 cut(s) 38, 511, 1053
CfoI GCGC 4 cut(s) 138, 239, 299, 366
Cfr10I RCCGGY 1 cut(s) 647
Cfr13I GGNCC 3 cut(s) 57, 559, 625
ClaI ATCGAT 1 cut(s) 585
CseI GACGC 1 cut(s) 489
Csp6I GTAC 3 cut(s) 372, 394, 944
CviAII CATG 5 cut(s) 42, 397, 607, 950, 980
CviQI GTAC 3 cut(s) 372, 394, 944
DdeI CTNAG 6 cut(s) 225, 390, 453, 531, 861, 922
DinI GGCGCC 2 cut(s) 238, 298
DpnI GATC 6 cut(s) 292, 402, 482, 541, 975, 1092
DpnII GATC 6 cut(s) 290, 400, 480, 539, 973, 1090
DrdI GACNNNNNNGTC 1 cut(s) 778
DriI GACNNNNNGTC 1 cut(s) 1120
DseDI GACNNNNNNGTC 1 cut(s) 778
EaeI YGGCCR 1 cut(s) 511
Eam1105I GACNNNNNGTC 1 cut(s) 1120
EciI GGCGGA 1 cut(s) 64
Ecl136II GAGCTC 1 cut(s) 432
Eco130I CCWWGG 2 cut(s) 41, 504
Eco24I GRGCYC 1 cut(s) 434
Eco47I GGWCC 1 cut(s) 57
Eco53kI GAGCTC 1 cut(s) 432
Eco57I CTGAAG 3 cut(s) 791, 897, 984
EcoICRI GAGCTC 1 cut(s) 432
EcoNI CCTNNNNNAGG 1 cut(s) 746
EcoO109I RGGNCCY 1 cut(s) 625
EcoRII CCWGG 2 cut(s) 23, 262
EcoT14I CCWWGG 2 cut(s) 41, 504
EcoT38I GRGCYC 1 cut(s) 434
EgeI GGCGCC 2 cut(s) 238, 298
EheI GGCGCC 2 cut(s) 238, 298
ErhI CCWWGG 2 cut(s) 41, 504
FaeI CATG 5 cut(s) 45, 400, 610, 953, 983
FaqI GGGAC 1 cut(s) 268
FatI CATG 5 cut(s) 41, 396, 606, 949, 979
FbaI TGATCA 1 cut(s) 1090
FblI GTMKAC 1 cut(s) 1068
Fnu4HI GCNGC 5 cut(s) 34, 81, 183, 186, 969
FokI GGATG 4 cut(s) 263, 423, 651, 788
FriOI GRGCYC 1 cut(s) 434
Fsp4HI GCNGC 5 cut(s) 34, 81, 183, 186, 969
FspBI CTAG 2 cut(s) 132, 146
GlaI GCGC 4 cut(s) 137, 238, 298, 365
GluI GCNGC 5 cut(s) 34, 81, 183, 186, 969
GsuI CTGGAG 2 cut(s) 109, 145
HaeII RGCGCY 2 cut(s) 240, 300
HaeIII GGCC 3 cut(s) 513, 561, 626
HapII CCGG 7 cut(s) 153, 294, 420, 518, 648, 763, 1099
HgaI GACGC 1 cut(s) 489
HhaI GCGC 4 cut(s) 138, 239, 299, 366
Hin1I GRCGYC 3 cut(s) 237, 297, 500
Hin1II CATG 5 cut(s) 45, 400, 610, 953, 983
Hin6I GCGC 4 cut(s) 136, 237, 297, 364
HinP1I GCGC 4 cut(s) 136, 237, 297, 364
HincII GTYRAC 2 cut(s) 207, 919
HindII GTYRAC 2 cut(s) 207, 919
HinfI GANTC 5 cut(s) 19, 149, 156, 350, 527
HpaII CCGG 7 cut(s) 153, 294, 420, 518, 648, 763, 1099
Hpy166II GTNNAC 4 cut(s) 57, 207, 919, 1069
Hpy188I TCNGA 7 cut(s) 89, 193, 290, 485, 544, 771, 1095
Hpy188III TCNNGA 8 cut(s) 153, 277, 452, 478, 636, 875, 955, 1125
Hpy8I GTNNAC 4 cut(s) 57, 207, 919, 1069
Hpy99I CGWCG 2 cut(s) 89, 408
HpyAV CCTTC 6 cut(s) 115, 274, 638, 815, 1072, 1076
HpyCH4III ACNGT 6 cut(s) 97, 491, 712, 896, 909, 1142
HpyCH4V TGCA 5 cut(s) 339, 707, 829, 999, 1020
HpyF10VI GCNNNNNNNGC 6 cut(s) 46, 80, 135, 142, 243, 506
HpyF3I CTNAG 6 cut(s) 225, 390, 453, 531, 861, 922
Hsp92I GRCGYC 3 cut(s) 237, 297, 500
Hsp92II CATG 5 cut(s) 45, 400, 610, 953, 983
HspAI GCGC 4 cut(s) 136, 237, 297, 364
KasI GGCGCC 2 cut(s) 236, 296
Kpn2I TCCGGA 1 cut(s) 152
Ksp22I TGATCA 1 cut(s) 1090
Kzo9I GATC 6 cut(s) 290, 400, 480, 539, 973, 1090
LmnI GCTCC 1 cut(s) 1060
Lsp1109I GCAGC 2 cut(s) 45, 980
LweI GCATC 3 cut(s) 629, 694, 775
MaeI CTAG 2 cut(s) 132, 146
MaeIII GTNAC 2 cut(s) 896, 1160
MalI GATC 6 cut(s) 292, 402, 482, 541, 975, 1092
MboI GATC 6 cut(s) 290, 400, 480, 539, 973, 1090
MboII GAAGA 6 cut(s) 164, 378, 683, 764, 859, 890
MfeI CAATTG 3 cut(s) 304, 600, 1015
MflI RGATCY 3 cut(s) 290, 480, 973
MhlI GDGCHC 2 cut(s) 434, 1057
MlsI TGGCCA 1 cut(s) 513
MluCI AATT 7 cut(s) 304, 340, 600, 882, 1015, 1021, 1038
MluNI TGGCCA 1 cut(s) 513
Mly113I GGCGCC 2 cut(s) 237, 297
MlyI GAGTC 2 cut(s) 143, 536
MmeI TCCRAC 3 cut(s) 67, 216, 1050
Mox20I TGGCCA 1 cut(s) 513
MroI TCCGGA 1 cut(s) 152
MroXI GAANNNNTTC 2 cut(s) 284, 756
MscI TGGCCA 1 cut(s) 513
MseI TTAA 2 cut(s) 462, 888
MslI CAYNNNNRTG 1 cut(s) 675
Msp20I TGGCCA 1 cut(s) 513
MspI CCGG 7 cut(s) 153, 294, 420, 518, 648, 763, 1099
MspR9I CCNGG 5 cut(s) 25, 264, 421, 518, 1099
MunI CAATTG 3 cut(s) 304, 600, 1015
MvaI CCWGG 2 cut(s) 25, 264
MvnI CGCG 1 cut(s) 138
MwoI GCNNNNNNNGC 6 cut(s) 46, 80, 135, 142, 243, 506
NarI GGCGCC 2 cut(s) 237, 297
NciI CCSGG 3 cut(s) 421, 518, 1099
NcoI CCATGG 1 cut(s) 41
NdeII GATC 6 cut(s) 290, 400, 480, 539, 973, 1090
NlaIII CATG 5 cut(s) 45, 400, 610, 953, 983
NlaIV GGNNCC 6 cut(s) 238, 292, 298, 482, 560, 855
NspI RCATGY 2 cut(s) 610, 953
NspV TTCGAA 1 cut(s) 845
PciI ACATGT 1 cut(s) 949
PcsI WCGNNNNNNNCGW 2 cut(s) 93, 350
PdmI GAANNNNTTC 2 cut(s) 284, 756
PfeI GAWTC 3 cut(s) 19, 156, 350
PflFI GACNNNGTC 1 cut(s) 197
PflMI CCANNNNNTGG 1 cut(s) 306
PfoI TCCNGGA 1 cut(s) 419
PkrI GCNGC 5 cut(s) 35, 82, 184, 187, 970
PleI GAGTC 2 cut(s) 143, 535
PluTI GGCGCC 2 cut(s) 240, 300
PpsI GAGTC 2 cut(s) 143, 535
PscI ACATGT 1 cut(s) 949
Psp124BI GAGCTC 1 cut(s) 434
Psp6I CCWGG 2 cut(s) 23, 262
PspGI CCWGG 2 cut(s) 23, 262
PspN4I GGNNCC 6 cut(s) 238, 292, 298, 482, 560, 855
PspPI GGNCC 3 cut(s) 57, 559, 625
PsuI RGATCY 3 cut(s) 290, 480, 973
PsyI GACNNNGTC 1 cut(s) 197
RsaI GTAC 3 cut(s) 373, 395, 945
RsaNI GTAC 3 cut(s) 372, 394, 944
RseI CAYNNNNRTG 1 cut(s) 675
SacI GAGCTC 1 cut(s) 434
SaqAI TTAA 2 cut(s) 462, 888
SatI GCNGC 5 cut(s) 34, 81, 183, 186, 969
Sau3AI GATC 6 cut(s) 290, 400, 480, 539, 973, 1090
Sau96I GGNCC 3 cut(s) 57, 559, 625
SchI GAGTC 2 cut(s) 143, 536
ScrFI CCNGG 5 cut(s) 25, 264, 421, 518, 1099
SduI GDGCHC 2 cut(s) 434, 1057
SfaNI GCATC 3 cut(s) 629, 694, 775
SfcI CTRYAG 1 cut(s) 1065
SfoI GGCGCC 2 cut(s) 238, 298
SfuI TTCGAA 1 cut(s) 845
SinI GGWCC 1 cut(s) 57
SmiMI CAYNNNNRTG 1 cut(s) 675
Sse9I AATT 7 cut(s) 304, 340, 600, 882, 1015, 1021, 1038
SsiI CCGC 4 cut(s) 49, 81, 183, 186
SspDI GGCGCC 2 cut(s) 236, 296
SspMI CTAG 2 cut(s) 132, 146
SstI GAGCTC 1 cut(s) 434
StyD4I CCNGG 5 cut(s) 23, 262, 419, 516, 1097
StyI CCWWGG 2 cut(s) 41, 504
TaaI ACNGT 6 cut(s) 97, 491, 712, 896, 909, 1142
TaqI TCGA 8 cut(s) 201, 344, 406, 585, 685, 845, 1124, 1170
TasI AATT 7 cut(s) 304, 340, 600, 882, 1015, 1021, 1038
TatI WGTACW 1 cut(s) 393
TauI GCSGC 3 cut(s) 83, 185, 188
TfiI GAWTC 3 cut(s) 19, 156, 350
Tru1I TTAA 2 cut(s) 462, 888
Tru9I TTAA 2 cut(s) 462, 888
TscAI CASTG 1 cut(s) 912
TseI GCWGC 2 cut(s) 33, 968
TspDTI ATGAA 1 cut(s) 603
TspRI CASTG 1 cut(s) 912
Tth111I GACNNNGTC 1 cut(s) 197
Van91I CCANNNNNTGG 1 cut(s) 306
VpaK11BI GGWCC 1 cut(s) 57
XagI CCTNNNNNAGG 1 cut(s) 746
XapI RAATTY 1 cut(s) 1038
XceI RCATGY 2 cut(s) 610, 953
XcmI CCANNNNNNNNNTGG 1 cut(s) 984
XmiI GTMKAC 1 cut(s) 1068
XmnI GAANNNNTTC 2 cut(s) 284, 756
XspI CTAG 2 cut(s) 132, 146
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.