MD04G1127600.v1.1

Hydrolyzes acetyl esters in homogalacturonan regions of pectin. In type I primary cell wall, galacturonic acid residues of pectin can be acetylated at the O-2 and O-3 positions. Decreasing the degree of acetylation of pectin gels in vitro alters their physical properties

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0001587
Physical Location & Seq
Forward (+)
21343592 .. 21347434
3843 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1127600.v1.1.491

Sequence Viewer

Length: 1326 bp
ATGGTGGTGGAGAAAGTCGGGTCAAATTCTATGGCAAATATTTCTGGGCTTCGCGGCCCGCTGTGGTGGATGCAGTGGGCCAAGCGTGGCTGGGCAATCGCCGCAGTCGGATTCGCCGTCTTCGTCTTCGCTCTCTCTGTCGCTTCCTACTATCGGAGTTCCGAGCCGGAGTCCAACACCGCGAACGCATTTCATCGCATCACCACCTCCGATTTGATAGACCTGACATTGTTGCACAGCGCCAAAGATAGAGGCGCCCTTTGTTTAGATGGAAGTTTGCCTGGCTACCATTTTCAAAAGGGATTTGGATCCGGCGCCAAAAATTGGCTACTTTTCATTGAGGGTGGAGGTTGGTGCAACACAATAGAATCGTGCATTTTGCGTAAACATATGCATTTAGGATCTTCCAAACATATGGGACGTCGAGTTCACTTTACTGGGATTTTAAGCCCTTACCCTGACCATAATCCTGGATTCTTTAACTGGAACCGAGTCAGAATACAGTATTGTGATGGCGCATTTATAGCCGGTCACCCGGATAACGAGTTGAAGAATGGAACGGAACTTTTCTTTAGGGGCCGGCTCATCTGGGAAGCAATTATGGATGAACTCTCGTCATTTGGCTTATCAAAAGCGGAACAGGCCCTTCTTTCAGGATGCTCTTCCGGTGGGTTGGCAGCTCTTATACATTGTGATGAATTTCGACATCTTTTGCCTAAGGATGCCACTGTCAAATGTCTAGGCGATGCGAGTTTTTTTCTTGATGAGAAAGATGTTCTCCACAATCCTACCATGAGATCTCTCTTTCGTGATGTTGTCAACCTTCAGGGGTTAGCGAAAAGTTTGAACAAGGATTGTGTTGCGAGAATGGAACCAGAAAGGTGTATCTTTCCTGAAGAAATGGTTAAAAACATCAAAACCCCTGTGTTCCATGTTCAATCAGCTTATGATTTTTGGCAAATACAAAATACCTTGATACCTAAAGCATCCGATCCACATAGCCACTGGCAAAAGTGCAGACTGAACATTTATAATTGCAATTCTACCCAGGTTGAAATACTACTAGGTTTCCGGGGATCTCTGCTGAAGGCGCTGAATGAATTCGAGAAAAATAAGGGAGGAGGAATGTTTATAAGTTCTTGCTTTATTCATTGCCAGACATTGATGACTGAGATATGGCATTCGCCGAAATCTTTAAGGATAAACAACAAGACAATTGCGGAGTCTGTGGGGGACTGGTACTTCAATCGAAGTGCAGCGAAGCTAATAGACTGCCCGTTTCCATGCAATCCGACCTGCTACAATGTGAATTTCACTCGAGGTTGA

Protein Analysis

442

Amino Acids

49.58

Weight (kDa)

8.52

Isoelectric Point (pI)

41.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PAE PF03283 71 - 433 3.1e-150 Pectinacetylesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1032, 1133
AatII GACGTC 1 cut(s) 424
Acc36I ACCTGC 1 cut(s) 1304
AccB1I GGYRCC 2 cut(s) 254, 314
AccB7I CCANNNNNTGG 1 cut(s) 324
AccII CGCG 2 cut(s) 54, 182
AciI CCGC 6 cut(s) 54, 59, 102, 180, 635, 1220
AclWI GGATC 5 cut(s) 303, 316, 409, 986, 1084
AcsI RAATTY 4 cut(s) 25, 698, 1100, 1309
AcuI CTGAAG 3 cut(s) 809, 915, 1106
AcyI GRCGYC 3 cut(s) 255, 315, 421
AfaI GTAC 1 cut(s) 1241
AfiI CCNNNNNNNGG 2 cut(s) 153, 324
AgsI TTSAA 6 cut(s) 296, 550, 847, 938, 1055, 1246
AjnI CCWGG 3 cut(s) 280, 469, 1047
AloI GAACNNNNNNTCC 2 cut(s) 411, 443
AluBI AGCT 3 cut(s) 680, 944, 1264
AluI AGCT 3 cut(s) 680, 944, 1264
AlwI GGATC 5 cut(s) 303, 316, 409, 986, 1084
Ama87I CYCGRG 1 cut(s) 1317
AoxI GGCC 4 cut(s) 55, 78, 577, 642
ApeKI GCWGC 2 cut(s) 677, 1256
ApoI RAATTY 4 cut(s) 25, 698, 1100, 1309
Asp700I GAANNNNTTC 1 cut(s) 1100
AspLEI GCGC 5 cut(s) 242, 257, 317, 518, 1093
AspS9I GGNCC 4 cut(s) 56, 78, 577, 643
AsuC2I CCSGG 2 cut(s) 536, 1073
AsuHPI GGTGA 2 cut(s) 193, 524
AvaI CYCGRG 1 cut(s) 1317
AxyI CCTNAGG 1 cut(s) 717
BamHI GGATCC 1 cut(s) 308
BanI GGYRCC 2 cut(s) 254, 314
BbsI GAAGAC 2 cut(s) 112, 118
BbvI GCAGC 2 cut(s) 689, 1268
BccI CCATC 2 cut(s) 263, 506
BceAI ACGGC 1 cut(s) 101
BciT130I CCWGG 3 cut(s) 282, 471, 1049
BcnI CCSGG 2 cut(s) 536, 1073
BfaI CTAG 2 cut(s) 740, 1064
BfoI RGCGCY 4 cut(s) 243, 258, 318, 1094
BfuAI ACCTGC 1 cut(s) 1304
BglII AGATCT 1 cut(s) 797
BisI GCNGC 4 cut(s) 55, 102, 678, 1257
BlsI GCNGC 4 cut(s) 56, 103, 679, 1258
Bme1390I CCNGG 5 cut(s) 282, 471, 536, 1049, 1073
BmeT110I CYCGRG 1 cut(s) 1317
BmgT120I GGNCC 4 cut(s) 56, 78, 577, 643
BmiI GGNNCC 6 cut(s) 256, 310, 316, 488, 578, 873
BmrFI CCNGG 5 cut(s) 282, 471, 536, 1049, 1073
BmrI ACTGGG 1 cut(s) 447
BmsI GCATC 6 cut(s) 60, 207, 647, 712, 736, 995
BmuI ACTGGG 1 cut(s) 447
BpiI GAAGAC 2 cut(s) 112, 118
BpuMI CCSGG 2 cut(s) 536, 1073
BsaBI GATNNNNATC 1 cut(s) 307
BsaHI GRCGYC 3 cut(s) 255, 315, 421
BsaJI CCNNGG 2 cut(s) 1047, 1072
BsaWI WCCGGW 1 cut(s) 665
Bsc4I CCNNNNNNNGG 2 cut(s) 153, 324
Bse118I RCCGGY 2 cut(s) 527, 579
Bse1I ACTGG 4 cut(s) 442, 488, 1010, 1241
Bse21I CCTNAGG 1 cut(s) 717
Bse3DI GCAATG 1 cut(s) 1150
Bse8I GATNNNNATC 1 cut(s) 307
BseBI CCWGG 3 cut(s) 282, 471, 1049
BseDI CCNNGG 2 cut(s) 1047, 1072
BseGI GGATG 5 cut(s) 75, 610, 662, 727, 986
BseJI GATNNNNATC 1 cut(s) 307
BseLI CCNNNNNNNGG 2 cut(s) 153, 324
BseMI GCAATG 1 cut(s) 1150
BseMII CTCAG 1 cut(s) 1161
BseNI ACTGG 4 cut(s) 442, 488, 1010, 1241
BseRI GAGGAG 1 cut(s) 1134
BseXI GCAGC 2 cut(s) 689, 1268
BseYI CCCAGC 1 cut(s) 90
BsgI GTGCAG 2 cut(s) 1036, 1275
Bsh1236I CGCG 2 cut(s) 54, 182
BshFI GGCC 4 cut(s) 57, 80, 579, 644
BshNI GGYRCC 2 cut(s) 254, 314
BsiHKCI CYCGRG 1 cut(s) 1317
BsiSI CCGG 7 cut(s) 167, 312, 528, 536, 580, 666, 1072
BslFI GGGAC 2 cut(s) 432, 1247
BslI CCNNNNNNNGG 2 cut(s) 153, 324
BsmFI GGGAC 2 cut(s) 432, 1247
BsmI GAATGC 1 cut(s) 1180
BsnI GGCC 4 cut(s) 57, 80, 579, 644
BsoBI CYCGRG 1 cut(s) 1317
Bsp143I GATC 5 cut(s) 308, 401, 797, 991, 1076
BspACI CCGC 6 cut(s) 54, 59, 102, 180, 635, 1220
BspANI GGCC 4 cut(s) 57, 80, 579, 644
BspCNI CTCAG 1 cut(s) 1162
BspFNI CGCG 2 cut(s) 54, 182
BspLI GGNNCC 6 cut(s) 256, 310, 316, 488, 578, 873
BspMI ACCTGC 1 cut(s) 1304
BspPI GGATC 5 cut(s) 303, 316, 409, 986, 1084
BspQI GCTCTTC 1 cut(s) 667
BspT107I GGYRCC 2 cut(s) 254, 314
BsrDI GCAATG 1 cut(s) 1150
BsrFI RCCGGY 2 cut(s) 527, 579
BsrI ACTGG 4 cut(s) 442, 488, 1010, 1241
BssAI RCCGGY 2 cut(s) 527, 579
BssECI CCNNGG 2 cut(s) 1047, 1072
BssMI GATC 5 cut(s) 308, 401, 797, 991, 1076
BssNI GRCGYC 3 cut(s) 255, 315, 421
Bst2UI CCWGG 3 cut(s) 282, 471, 1049
Bst4CI ACNGT 2 cut(s) 504, 730
Bst6I CTCTTC 1 cut(s) 667
BstACI GRCGYC 3 cut(s) 255, 315, 421
BstC8I GCNNGC 2 cut(s) 59, 581
BstDEI CTNAG 2 cut(s) 717, 1170
BstEII GGTNACC 1 cut(s) 530
BstF5I GGATG 5 cut(s) 75, 610, 662, 727, 986
BstFNI CGCG 2 cut(s) 54, 182
BstH2I RGCGCY 4 cut(s) 243, 258, 318, 1094
BstHHI GCGC 5 cut(s) 242, 257, 317, 518, 1093
BstKTI GATC 5 cut(s) 311, 404, 800, 994, 1079
BstMBI GATC 5 cut(s) 308, 401, 797, 991, 1076
BstMWI GCNNNNNNNGC 4 cut(s) 101, 524, 641, 1090
BstNI CCWGG 3 cut(s) 282, 471, 1049
BstPI GGTNACC 1 cut(s) 530
BstSCI CCNGG 5 cut(s) 280, 469, 534, 1047, 1071
BstUI CGCG 2 cut(s) 54, 182
BstV1I GCAGC 2 cut(s) 689, 1268
BstV2I GAAGAC 2 cut(s) 112, 118
BstX2I RGATCY 4 cut(s) 308, 401, 797, 1076
BstXI CCANNNNNNTGG 2 cut(s) 415, 470
BstYI RGATCY 4 cut(s) 308, 401, 797, 1076
Bsu36I CCTNAGG 1 cut(s) 717
BsuRI GGCC 4 cut(s) 57, 80, 579, 644
BtgZI GCGATG 2 cut(s) 179, 759
BtsCI GGATG 5 cut(s) 75, 610, 662, 727, 986
BtsI GCAGTG 1 cut(s) 80
BtsIMutI CAGTG 3 cut(s) 80, 726, 1003
BveI ACCTGC 1 cut(s) 1304
Cac8I GCNNGC 2 cut(s) 59, 581
CfoI GCGC 5 cut(s) 242, 257, 317, 518, 1093
Cfr10I RCCGGY 2 cut(s) 527, 579
Cfr13I GGNCC 4 cut(s) 56, 78, 577, 643
Csp6I GTAC 1 cut(s) 1240
CviAII CATG 3 cut(s) 793, 932, 1284
CviQI GTAC 1 cut(s) 1240
DdeI CTNAG 2 cut(s) 717, 1170
DinI GGCGCC 2 cut(s) 256, 316
DpnI GATC 5 cut(s) 310, 403, 799, 993, 1078
DpnII GATC 5 cut(s) 308, 401, 797, 991, 1076
Eam1104I CTCTTC 1 cut(s) 667
EarI CTCTTC 1 cut(s) 667
Eco57I CTGAAG 3 cut(s) 809, 915, 1106
Eco81I CCTNAGG 1 cut(s) 717
Eco88I CYCGRG 1 cut(s) 1317
Eco91I GGTNACC 1 cut(s) 530
EcoO109I RGGNCCY 1 cut(s) 643
EcoO65I GGTNACC 1 cut(s) 530
EcoRI GAATTC 1 cut(s) 1100
EcoRII CCWGG 3 cut(s) 280, 469, 1047
EcoT22I ATGCAT 1 cut(s) 396
EgeI GGCGCC 2 cut(s) 256, 316
EheI GGCGCC 2 cut(s) 256, 316
FaeI CATG 3 cut(s) 796, 935, 1287
FaqI GGGAC 2 cut(s) 432, 1247
FatI CATG 3 cut(s) 792, 931, 1283
FauI CCCGC 1 cut(s) 66
FauNDI CATATG 2 cut(s) 390, 414
Fnu4HI GCNGC 4 cut(s) 55, 102, 678, 1257
FokI GGATG 5 cut(s) 82, 617, 669, 734, 973
Fsp4HI GCNGC 4 cut(s) 55, 102, 678, 1257
FspBI CTAG 2 cut(s) 740, 1064
GlaI GCGC 5 cut(s) 241, 256, 316, 517, 1092
GluI GCNGC 4 cut(s) 55, 102, 678, 1257
GsaI CCCAGC 1 cut(s) 94
HaeII RGCGCY 4 cut(s) 243, 258, 318, 1094
HaeIII GGCC 4 cut(s) 57, 80, 579, 644
HapII CCGG 7 cut(s) 167, 312, 528, 536, 580, 666, 1072
HhaI GCGC 5 cut(s) 242, 257, 317, 518, 1093
Hin1I GRCGYC 3 cut(s) 255, 315, 421
Hin1II CATG 3 cut(s) 796, 935, 1287
Hin6I GCGC 5 cut(s) 240, 255, 315, 516, 1091
HinP1I GCGC 5 cut(s) 240, 255, 315, 516, 1091
HincII GTYRAC 1 cut(s) 820
HindII GTYRAC 1 cut(s) 820
HinfI GANTC 6 cut(s) 111, 170, 368, 474, 492, 1223
HpaII CCGG 7 cut(s) 167, 312, 528, 536, 580, 666, 1072
HphI GGTGA 2 cut(s) 193, 524
Hpy166II GTNNAC 3 cut(s) 386, 430, 820
Hpy188I TCNGA 7 cut(s) 110, 156, 163, 211, 497, 991, 1293
Hpy188III TCNNGA 5 cut(s) 654, 761, 809, 893, 1105
Hpy8I GTNNAC 3 cut(s) 386, 430, 820
Hpy99I CGWCG 1 cut(s) 426
HpyAV CCTTC 3 cut(s) 656, 833, 1081
HpyCH4III ACNGT 2 cut(s) 504, 730
HpyCH4IV ACGT 1 cut(s) 421
HpyCH4V TGCA 9 cut(s) 73, 235, 357, 375, 394, 1017, 1038, 1256, 1287
HpyF10VI GCNNNNNNNGC 4 cut(s) 101, 524, 641, 1090
HpyF3I CTNAG 2 cut(s) 717, 1170
HpySE526I ACGT 1 cut(s) 421
Hsp92I GRCGYC 3 cut(s) 255, 315, 421
Hsp92II CATG 3 cut(s) 796, 935, 1287
HspAI GCGC 5 cut(s) 240, 255, 315, 516, 1091
KasI GGCGCC 2 cut(s) 254, 314
KroI GCCGGC 1 cut(s) 579
KroNI GCCGGC 1 cut(s) 581
Kzo9I GATC 5 cut(s) 308, 401, 797, 991, 1076
LguI GCTCTTC 1 cut(s) 667
Lsp1109I GCAGC 2 cut(s) 689, 1268
LweI GCATC 6 cut(s) 60, 207, 647, 712, 736, 995
MaeI CTAG 2 cut(s) 740, 1064
MaeII ACGT 1 cut(s) 421
MaeIII GTNAC 1 cut(s) 530
MalI GATC 5 cut(s) 310, 403, 799, 993, 1078
MboI GATC 5 cut(s) 308, 401, 797, 991, 1076
MboII GAAGA 6 cut(s) 112, 118, 396, 562, 654, 908
MfeI CAATTG 1 cut(s) 1215
MflI RGATCY 4 cut(s) 308, 401, 797, 1076
MluCI AATT 9 cut(s) 25, 322, 597, 698, 1033, 1039, 1100, 1215, 1309
Mly113I GGCGCC 2 cut(s) 255, 315
MlyI GAGTC 3 cut(s) 179, 501, 1232
MmeI TCCRAC 3 cut(s) 88, 198, 1316
MnlI CCTC 7 cut(s) 217, 245, 334, 341, 1112, 1115, 1313
Mph1103I ATGCAT 1 cut(s) 396
MroNI GCCGGC 1 cut(s) 579
MroXI GAANNNNTTC 1 cut(s) 1100
MseI TTAA 4 cut(s) 446, 480, 906, 1196
MslI CAYNNNNRTG 1 cut(s) 693
MspA1I CMGCKG 1 cut(s) 61
MspI CCGG 7 cut(s) 167, 312, 528, 536, 580, 666, 1072
MspR9I CCNGG 5 cut(s) 282, 471, 536, 1049, 1073
MunI CAATTG 1 cut(s) 1215
Mva1269I GAATGC 1 cut(s) 1180
MvaI CCWGG 3 cut(s) 282, 471, 1049
MvnI CGCG 2 cut(s) 54, 182
MwoI GCNNNNNNNGC 4 cut(s) 101, 524, 641, 1090
NaeI GCCGGC 1 cut(s) 581
NarI GGCGCC 2 cut(s) 255, 315
NciI CCSGG 2 cut(s) 536, 1073
NdeI CATATG 2 cut(s) 390, 414
NdeII GATC 5 cut(s) 308, 401, 797, 991, 1076
NgoMIV GCCGGC 1 cut(s) 579
NlaIII CATG 3 cut(s) 796, 935, 1287
NlaIV GGNNCC 6 cut(s) 256, 310, 316, 488, 578, 873
NmuCI GTSAC 1 cut(s) 530
NsiI ATGCAT 1 cut(s) 396
PaeR7I CTCGAG 1 cut(s) 1317
PciSI GCTCTTC 1 cut(s) 667
PcsI WCGNNNNNNNCGW 2 cut(s) 114, 120
PctI GAATGC 1 cut(s) 1180
PdiI GCCGGC 1 cut(s) 581
PdmI GAANNNNTTC 1 cut(s) 1100
PfeI GAWTC 3 cut(s) 111, 368, 474
PflMI CCANNNNNTGG 1 cut(s) 324
PfoI TCCNGGA 1 cut(s) 469
PkrI GCNGC 4 cut(s) 56, 103, 679, 1258
PleI GAGTC 3 cut(s) 178, 500, 1231
PluTI GGCGCC 2 cut(s) 258, 318
PpsI GAGTC 3 cut(s) 178, 500, 1231
PsiI TTATAA 2 cut(s) 1032, 1133
Psp6I CCWGG 3 cut(s) 280, 469, 1047
PspEI GGTNACC 1 cut(s) 530
PspFI CCCAGC 1 cut(s) 90
PspGI CCWGG 3 cut(s) 280, 469, 1047
PspN4I GGNNCC 6 cut(s) 256, 310, 316, 488, 578, 873
PspPI GGNCC 4 cut(s) 56, 78, 577, 643
PspXI VCTCGAGB 1 cut(s) 1317
PsuI RGATCY 4 cut(s) 308, 401, 797, 1076
RsaI GTAC 1 cut(s) 1241
RsaNI GTAC 1 cut(s) 1240
RseI CAYNNNNRTG 1 cut(s) 693
SapI GCTCTTC 1 cut(s) 667
SaqAI TTAA 4 cut(s) 446, 480, 906, 1196
SatI GCNGC 4 cut(s) 55, 102, 678, 1257
Sau3AI GATC 5 cut(s) 308, 401, 797, 991, 1076
Sau96I GGNCC 4 cut(s) 56, 78, 577, 643
SchI GAGTC 3 cut(s) 179, 501, 1232
ScrFI CCNGG 5 cut(s) 282, 471, 536, 1049, 1073
SfaNI GCATC 6 cut(s) 60, 207, 647, 712, 736, 995
SfoI GGCGCC 2 cut(s) 256, 316
Sfr274I CTCGAG 1 cut(s) 1317
SlaI CTCGAG 1 cut(s) 1317
SmiMI CAYNNNNRTG 1 cut(s) 693
SmlI CTYRAG 1 cut(s) 1317
SmoI CTYRAG 1 cut(s) 1317
Sse9I AATT 9 cut(s) 25, 322, 597, 698, 1033, 1039, 1100, 1215, 1309
SsiI CCGC 6 cut(s) 54, 59, 102, 180, 635, 1220
SspDI GGCGCC 2 cut(s) 254, 314
SspI AATATT 1 cut(s) 40
SspMI CTAG 2 cut(s) 740, 1064
StyD4I CCNGG 5 cut(s) 280, 469, 534, 1047, 1071
TaaI ACNGT 2 cut(s) 504, 730
TaiI ACGT 1 cut(s) 424
TaqI TCGA 5 cut(s) 424, 703, 1104, 1249, 1318
TasI AATT 9 cut(s) 25, 322, 597, 698, 1033, 1039, 1100, 1215, 1309
TauI GCSGC 2 cut(s) 57, 104
TfiI GAWTC 3 cut(s) 111, 368, 474
Tru1I TTAA 4 cut(s) 446, 480, 906, 1196
Tru9I TTAA 4 cut(s) 446, 480, 906, 1196
TscAI CASTG 3 cut(s) 80, 733, 1010
TseFI GTSAC 1 cut(s) 530
TseI GCWGC 2 cut(s) 677, 1256
Tsp45I GTSAC 1 cut(s) 530
TspDTI ATGAA 6 cut(s) 182, 325, 621, 711, 1113, 1139
TspGWI ACGGA 1 cut(s) 575
TspRI CASTG 3 cut(s) 80, 733, 1010
Van91I CCANNNNNTGG 1 cut(s) 324
XapI RAATTY 4 cut(s) 25, 698, 1100, 1309
XcmI CCANNNNNNNNNTGG 1 cut(s) 1002
XhoI CTCGAG 1 cut(s) 1317
XmnI GAANNNNTTC 1 cut(s) 1100
XspI CTAG 2 cut(s) 740, 1064
ZraI GACGTC 1 cut(s) 422
Zsp2I ATGCAT 1 cut(s) 396
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.