Hydrolyzes acetyl esters in homogalacturonan regions of pectin. In type I primary cell wall, galacturonic acid residues of pectin can be acetylated at the O-2 and O-3 positions. Decreasing the degree of acetylation of pectin gels in vitro alters their physical properties
Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 1 cut(s) | 1364 |
| AarI | CACCTGC | 1 cut(s) | 1535 |
| AasI | GACNNNNNNGTC | 1 cut(s) | 1027 |
| Acc16I | TGCGCA | 1 cut(s) | 356 |
| Acc36I | ACCTGC | 3 cut(s) | 971, 1180, 1535 |
| AccB1I | GGYRCC | 2 cut(s) | 374, 545 |
| AccB7I | CCANNNNNTGG | 2 cut(s) | 270, 555 |
| AccII | CGCG | 1 cut(s) | 749 |
| AciI | CCGC | 6 cut(s) | 301, 321, 324, 460, 501, 1018 |
| AclWI | GGATC | 4 cut(s) | 534, 547, 657, 1217 |
| AcoI | YGGCCR | 1 cut(s) | 760 |
| AcsI | RAATTY | 4 cut(s) | 704, 1331, 1366, 1540 |
| AcuI | CTGAAG | 3 cut(s) | 1040, 1146, 1233 |
| AcyI | GRCGYC | 2 cut(s) | 375, 546 |
| AfaI | GTAC | 4 cut(s) | 382, 622, 1194, 1472 |
| AfiI | CCNNNNNNNGG | 7 cut(s) | 270, 555, 668, 669, 785, 997, 1407 |
| AgsI | TTSAA | 3 cut(s) | 599, 1078, 1095 |
| AjiI | CACGTC | 1 cut(s) | 1392 |
| AjnI | CCWGG | 2 cut(s) | 161, 511 |
| AluBI | AGCT | 6 cut(s) | 53, 681, 814, 1109, 1175, 1282 |
| AluI | AGCT | 6 cut(s) | 53, 681, 814, 1109, 1175, 1282 |
| Alw21I | GWGCWC | 2 cut(s) | 118, 683 |
| Alw26I | GTCTC | 2 cut(s) | 1121, 1397 |
| AlwI | GGATC | 4 cut(s) | 534, 547, 657, 1217 |
| AlwNI | CAGNNNCTG | 2 cut(s) | 738, 1457 |
| AoxI | GGCC | 4 cut(s) | 195, 760, 808, 873 |
| ApeKI | GCWGC | 5 cut(s) | 171, 352, 559, 1015, 1217 |
| ApoI | RAATTY | 4 cut(s) | 704, 1331, 1366, 1540 |
| ArsI | GACNNNNNNTTYG | 4 cut(s) | 1457, 1489, 1489, 1521 |
| AseI | ATTAAT | 1 cut(s) | 1146 |
| Asp700I | GAANNNNTTC | 2 cut(s) | 533, 1005 |
| AspLEI | GCGC | 6 cut(s) | 276, 357, 377, 548, 615, 749 |
| AspS9I | GGNCC | 4 cut(s) | 195, 794, 808, 874 |
| AsuC2I | CCSGG | 1 cut(s) | 767 |
| AsuHPI | GGTGA | 1 cut(s) | 1407 |
| AvaII | GGWCC | 1 cut(s) | 794 |
| AxyI | CCTNAGG | 1 cut(s) | 948 |
| BaeI | ACNNNNGTAYC | 2 cut(s) | 1462, 1495 |
| BalI | TGGCCA | 1 cut(s) | 762 |
| BamHI | GGATCC | 1 cut(s) | 539 |
| BanI | GGYRCC | 2 cut(s) | 374, 545 |
| BanII | GRGCYC | 1 cut(s) | 683 |
| BauI | CACGAG | 1 cut(s) | 1557 |
| BbsI | GAAGAC | 1 cut(s) | 627 |
| Bbv12I | GWGCWC | 2 cut(s) | 118, 683 |
| BbvI | GCAGC | 5 cut(s) | 183, 339, 546, 1027, 1229 |
| BccI | CCATC | 7 cut(s) | 133, 186, 399, 697, 737, 1058, 1093 |
| BceAI | ACGGC | 1 cut(s) | 203 |
| BciT130I | CCWGG | 2 cut(s) | 163, 513 |
| BcnI | CCSGG | 1 cut(s) | 767 |
| BcoDI | GTCTC | 2 cut(s) | 1121, 1397 |
| BfaI | CTAG | 1 cut(s) | 629 |
| BfoI | RGCGCY | 2 cut(s) | 378, 549 |
| BfuAI | ACCTGC | 3 cut(s) | 971, 1180, 1535 |
| BisI | GCNGC | 7 cut(s) | 172, 321, 324, 353, 560, 1016, 1218 |
| BlsI | GCNGC | 7 cut(s) | 173, 322, 325, 354, 561, 1017, 1219 |
| Bme1390I | CCNGG | 3 cut(s) | 163, 513, 767 |
| Bme18I | GGWCC | 1 cut(s) | 794 |
| BmgBI | CACGTC | 1 cut(s) | 1392 |
| BmgT120I | GGNCC | 4 cut(s) | 195, 794, 808, 874 |
| BmiI | GGNNCC | 5 cut(s) | 376, 541, 547, 809, 1104 |
| BmrFI | CCNGG | 3 cut(s) | 163, 513, 767 |
| BmrI | ACTGGG | 2 cut(s) | 217, 1148 |
| BmsI | GCATC | 3 cut(s) | 878, 943, 967 |
| BmuI | ACTGGG | 2 cut(s) | 217, 1148 |
| BpiI | GAAGAC | 1 cut(s) | 627 |
| BpmI | CTGGAG | 1 cut(s) | 995 |
| BpuMI | CCSGG | 1 cut(s) | 767 |
| BsaBI | GATNNNNATC | 1 cut(s) | 538 |
| BsaHI | GRCGYC | 2 cut(s) | 375, 546 |
| BsaJI | CCNNGG | 2 cut(s) | 161, 511 |
| BsaWI | WCCGGW | 1 cut(s) | 118 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 1231, 1261 |
| Bsc4I | CCNNNNNNNGG | 7 cut(s) | 270, 555, 668, 669, 785, 997, 1407 |
| Bse1I | ACTGG | 5 cut(s) | 77, 212, 719, 1154, 1472 |
| Bse21I | CCTNAGG | 1 cut(s) | 948 |
| Bse3DI | GCAATG | 1 cut(s) | 1381 |
| Bse8I | GATNNNNATC | 1 cut(s) | 538 |
| BseBI | CCWGG | 2 cut(s) | 163, 513 |
| BseDI | CCNNGG | 2 cut(s) | 161, 511 |
| BseGI | GGATG | 7 cut(s) | 391, 685, 841, 893, 958, 1050, 1104 |
| BseJI | GATNNNNATC | 1 cut(s) | 538 |
| BseLI | CCNNNNNNNGG | 7 cut(s) | 270, 555, 668, 669, 785, 997, 1407 |
| BseMI | GCAATG | 1 cut(s) | 1381 |
| BseNI | ACTGG | 5 cut(s) | 77, 212, 719, 1154, 1472 |
| BseRI | GAGGAG | 1 cut(s) | 248 |
| BseXI | GCAGC | 5 cut(s) | 183, 339, 546, 1027, 1229 |
| BseYI | CCCAGC | 2 cut(s) | 1171, 1274 |
| Bsh1236I | CGCG | 1 cut(s) | 749 |
| BshFI | GGCC | 4 cut(s) | 197, 762, 810, 875 |
| BshNI | GGYRCC | 2 cut(s) | 374, 545 |
| BsiHKAI | GWGCWC | 2 cut(s) | 118, 683 |
| BsiSI | CCGG | 3 cut(s) | 119, 543, 767 |
| BslFI | GGGAC | 1 cut(s) | 517 |
| BslI | CCNNNNNNNGG | 7 cut(s) | 270, 555, 668, 669, 785, 997, 1407 |
| BsmAI | GTCTC | 2 cut(s) | 1121, 1397 |
| BsmFI | GGGAC | 1 cut(s) | 517 |
| BsmI | GAATGC | 1 cut(s) | 1411 |
| BsnI | GGCC | 4 cut(s) | 197, 762, 810, 875 |
| Bsp1286I | GDGCHC | 2 cut(s) | 118, 683 |
| Bsp143I | GATC | 3 cut(s) | 539, 649, 1222 |
| BspACI | CCGC | 6 cut(s) | 301, 321, 324, 460, 501, 1018 |
| BspANI | GGCC | 4 cut(s) | 197, 762, 810, 875 |
| BspFNI | CGCG | 1 cut(s) | 749 |
| BspLI | GGNNCC | 5 cut(s) | 376, 541, 547, 809, 1104 |
| BspMI | ACCTGC | 3 cut(s) | 971, 1180, 1535 |
| BspPI | GGATC | 4 cut(s) | 534, 547, 657, 1217 |
| BspT107I | GGYRCC | 2 cut(s) | 374, 545 |
| BsrDI | GCAATG | 1 cut(s) | 1381 |
| BsrI | ACTGG | 5 cut(s) | 77, 212, 719, 1154, 1472 |
| BssECI | CCNNGG | 2 cut(s) | 161, 511 |
| BssMI | GATC | 3 cut(s) | 539, 649, 1222 |
| BssNI | GRCGYC | 2 cut(s) | 375, 546 |
| BssSI | CACGAG | 1 cut(s) | 1557 |
| Bst2BI | CACGAG | 1 cut(s) | 1557 |
| Bst2UI | CCWGG | 2 cut(s) | 163, 513 |
| Bst4CI | ACNGT | 4 cut(s) | 235, 740, 961, 1505 |
| BstACI | GRCGYC | 2 cut(s) | 375, 546 |
| BstC8I | GCNNGC | 4 cut(s) | 176, 760, 812, 1280 |
| BstDEI | CTNAG | 3 cut(s) | 780, 948, 1110 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 995 |
| BstF5I | GGATG | 7 cut(s) | 391, 685, 841, 893, 958, 1050, 1104 |
| BstFNI | CGCG | 1 cut(s) | 749 |
| BstH2I | RGCGCY | 2 cut(s) | 378, 549 |
| BstHHI | GCGC | 6 cut(s) | 276, 357, 377, 548, 615, 749 |
| BstKTI | GATC | 3 cut(s) | 542, 652, 1225 |
| BstMAI | GTCTC | 2 cut(s) | 1121, 1397 |
| BstMBI | GATC | 3 cut(s) | 539, 649, 1222 |
| BstMWI | GCNNNNNNNGC | 4 cut(s) | 122, 184, 755, 1275 |
| BstNI | CCWGG | 2 cut(s) | 163, 513 |
| BstSCI | CCNGG | 3 cut(s) | 161, 511, 765 |
| BstUI | CGCG | 1 cut(s) | 749 |
| BstV1I | GCAGC | 5 cut(s) | 183, 339, 546, 1027, 1229 |
| BstV2I | GAAGAC | 1 cut(s) | 627 |
| BstX2I | RGATCY | 2 cut(s) | 539, 1222 |
| BstYI | RGATCY | 2 cut(s) | 539, 1222 |
| Bsu36I | CCTNAGG | 1 cut(s) | 948 |
| BsuRI | GGCC | 4 cut(s) | 197, 762, 810, 875 |
| BtrI | CACGTC | 1 cut(s) | 1392 |
| BtsCI | GGATG | 7 cut(s) | 391, 685, 841, 893, 958, 1050, 1104 |
| BtsIMutI | CAGTG | 4 cut(s) | 95, 156, 1161, 1322 |
| BveI | ACCTGC | 3 cut(s) | 971, 1180, 1535 |
| Cac8I | GCNNGC | 4 cut(s) | 176, 760, 812, 1280 |
| CaiI | CAGNNNCTG | 2 cut(s) | 738, 1457 |
| CfoI | GCGC | 6 cut(s) | 276, 357, 377, 548, 615, 749 |
| Cfr13I | GGNCC | 4 cut(s) | 195, 794, 808, 874 |
| CseI | GACGC | 1 cut(s) | 738 |
| Csp6I | GTAC | 4 cut(s) | 381, 621, 1193, 1471 |
| CviAII | CATG | 6 cut(s) | 646, 1229, 1295, 1371, 1407, 1515 |
| CviQI | GTAC | 4 cut(s) | 381, 621, 1193, 1471 |
| DdeI | CTNAG | 3 cut(s) | 780, 948, 1110 |
| DinI | GGCGCC | 2 cut(s) | 376, 547 |
| DpnI | GATC | 3 cut(s) | 541, 651, 1224 |
| DpnII | GATC | 3 cut(s) | 539, 649, 1222 |
| DrdI | GACNNNNNNGTC | 1 cut(s) | 1027 |
| DseDI | GACNNNNNNGTC | 1 cut(s) | 1027 |
| EaeI | YGGCCR | 1 cut(s) | 760 |
| Ecl136II | GAGCTC | 1 cut(s) | 681 |
| Eco24I | GRGCYC | 1 cut(s) | 683 |
| Eco47I | GGWCC | 1 cut(s) | 794 |
| Eco53kI | GAGCTC | 1 cut(s) | 681 |
| Eco57I | CTGAAG | 3 cut(s) | 1040, 1146, 1233 |
| Eco81I | CCTNAGG | 1 cut(s) | 948 |
| EcoICRI | GAGCTC | 1 cut(s) | 681 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 995 |
| EcoO109I | RGGNCCY | 2 cut(s) | 794, 874 |
| EcoRI | GAATTC | 2 cut(s) | 704, 1331 |
| EcoRII | CCWGG | 2 cut(s) | 161, 511 |
| EcoT38I | GRGCYC | 1 cut(s) | 683 |
| EgeI | GGCGCC | 2 cut(s) | 376, 547 |
| EheI | GGCGCC | 2 cut(s) | 376, 547 |
| FaeI | CATG | 6 cut(s) | 649, 1232, 1298, 1374, 1410, 1518 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 1103, 1135 |
| FaqI | GGGAC | 1 cut(s) | 517 |
| FatI | CATG | 6 cut(s) | 645, 1228, 1294, 1370, 1406, 1514 |
| FauI | CCCGC | 3 cut(s) | 308, 467, 494 |
| FauNDI | CATATG | 1 cut(s) | 1536 |
| Fnu4HI | GCNGC | 7 cut(s) | 172, 321, 324, 353, 560, 1016, 1218 |
| FokI | GGATG | 7 cut(s) | 378, 672, 848, 900, 965, 1037, 1111 |
| FriOI | GRGCYC | 1 cut(s) | 683 |
| Fsp4HI | GCNGC | 7 cut(s) | 172, 321, 324, 353, 560, 1016, 1218 |
| FspBI | CTAG | 1 cut(s) | 629 |
| FspI | TGCGCA | 1 cut(s) | 356 |
| GlaI | GCGC | 6 cut(s) | 275, 356, 376, 547, 614, 748 |
| GluI | GCNGC | 7 cut(s) | 172, 321, 324, 353, 560, 1016, 1218 |
| GsaI | CCCAGC | 2 cut(s) | 1175, 1278 |
| GsuI | CTGGAG | 1 cut(s) | 995 |
| HaeII | RGCGCY | 2 cut(s) | 378, 549 |
| HaeIII | GGCC | 4 cut(s) | 197, 762, 810, 875 |
| HapII | CCGG | 3 cut(s) | 119, 543, 767 |
| HgaI | GACGC | 1 cut(s) | 738 |
| HhaI | GCGC | 6 cut(s) | 276, 357, 377, 548, 615, 749 |
| Hin1I | GRCGYC | 2 cut(s) | 375, 546 |
| Hin1II | CATG | 6 cut(s) | 649, 1232, 1298, 1374, 1410, 1518 |
| Hin6I | GCGC | 6 cut(s) | 274, 355, 375, 546, 613, 747 |
| HinP1I | GCGC | 6 cut(s) | 274, 355, 375, 546, 613, 747 |
| HincII | GTYRAC | 2 cut(s) | 340, 1168 |
| HindII | GTYRAC | 2 cut(s) | 340, 1168 |
| HindIII | AAGCTT | 1 cut(s) | 51 |
| HinfI | GANTC | 7 cut(s) | 157, 287, 534, 599, 910, 1307, 1454 |
| HpaII | CCGG | 3 cut(s) | 119, 543, 767 |
| HphI | GGTGA | 1 cut(s) | 1407 |
| Hpy166II | GTNNAC | 3 cut(s) | 340, 1168, 1250 |
| Hpy188I | TCNGA | 4 cut(s) | 227, 292, 539, 734 |
| Hpy188III | TCNNGA | 6 cut(s) | 526, 701, 885, 1124, 1204, 1557 |
| Hpy8I | GTNNAC | 3 cut(s) | 340, 1168, 1250 |
| Hpy99I | CGWCG | 3 cut(s) | 224, 227, 657 |
| HpyAV | CCTTC | 7 cut(s) | 253, 337, 523, 763, 887, 1064, 1343 |
| HpyCH4III | ACNGT | 4 cut(s) | 235, 740, 961, 1505 |
| HpyCH4IV | ACGT | 1 cut(s) | 1391 |
| HpyCH4V | TGCA | 7 cut(s) | 588, 956, 980, 1269, 1451, 1487, 1518 |
| HpyF10VI | GCNNNNNNNGC | 4 cut(s) | 122, 184, 755, 1275 |
| HpyF3I | CTNAG | 3 cut(s) | 780, 948, 1110 |
| HpySE526I | ACGT | 1 cut(s) | 1391 |
| Hsp92I | GRCGYC | 2 cut(s) | 375, 546 |
| Hsp92II | CATG | 6 cut(s) | 649, 1232, 1298, 1374, 1410, 1518 |
| HspAI | GCGC | 6 cut(s) | 274, 355, 375, 546, 613, 747 |
| KasI | GGCGCC | 2 cut(s) | 374, 545 |
| Kzo9I | GATC | 3 cut(s) | 539, 649, 1222 |
| Lsp1109I | GCAGC | 5 cut(s) | 183, 339, 546, 1027, 1229 |
| LweI | GCATC | 3 cut(s) | 878, 943, 967 |
| MaeI | CTAG | 1 cut(s) | 629 |
| MaeII | ACGT | 1 cut(s) | 1391 |
| MalI | GATC | 3 cut(s) | 541, 651, 1224 |
| MboI | GATC | 3 cut(s) | 539, 649, 1222 |
| MboII | GAAGA | 6 cut(s) | 201, 302, 627, 932, 1013, 1139 |
| MfeI | CAATTG | 5 cut(s) | 134, 553, 849, 1264, 1446 |
| MflI | RGATCY | 2 cut(s) | 539, 1222 |
| MhlI | GDGCHC | 2 cut(s) | 118, 683 |
| MlsI | TGGCCA | 1 cut(s) | 762 |
| MluNI | TGGCCA | 1 cut(s) | 762 |
| Mly113I | GGCGCC | 2 cut(s) | 375, 546 |
| MlyI | GAGTC | 3 cut(s) | 281, 904, 1463 |
| MmeI | TCCRAC | 2 cut(s) | 205, 288 |
| Mox20I | TGGCCA | 1 cut(s) | 762 |
| MroXI | GAANNNNTTC | 2 cut(s) | 533, 1005 |
| MscI | TGGCCA | 1 cut(s) | 762 |
| MseI | TTAA | 7 cut(s) | 6, 12, 63, 711, 1137, 1146, 1284 |
| MslI | CAYNNNNRTG | 2 cut(s) | 924, 1513 |
| Msp20I | TGGCCA | 1 cut(s) | 762 |
| MspA1I | CMGCKG | 1 cut(s) | 1018 |
| MspI | CCGG | 3 cut(s) | 119, 543, 767 |
| MspR9I | CCNGG | 3 cut(s) | 163, 513, 767 |
| MunI | CAATTG | 5 cut(s) | 134, 553, 849, 1264, 1446 |
| Mva1269I | GAATGC | 1 cut(s) | 1411 |
| MvaI | CCWGG | 2 cut(s) | 163, 513 |
| MvnI | CGCG | 1 cut(s) | 749 |
| MwoI | GCNNNNNNNGC | 4 cut(s) | 122, 184, 755, 1275 |
| NarI | GGCGCC | 2 cut(s) | 375, 546 |
| NciI | CCSGG | 1 cut(s) | 767 |
| NdeI | CATATG | 1 cut(s) | 1536 |
| NdeII | GATC | 3 cut(s) | 539, 649, 1222 |
| NlaIII | CATG | 6 cut(s) | 649, 1232, 1298, 1374, 1410, 1518 |
| NlaIV | GGNNCC | 5 cut(s) | 376, 541, 547, 809, 1104 |
| NsbI | TGCGCA | 1 cut(s) | 356 |
| PaqCI | CACCTGC | 1 cut(s) | 1535 |
| PcsI | WCGNNNNNNNCGW | 2 cut(s) | 231, 599 |
| PctI | GAATGC | 1 cut(s) | 1411 |
| PdmI | GAANNNNTTC | 2 cut(s) | 533, 1005 |
| PfeI | GAWTC | 4 cut(s) | 157, 534, 599, 1307 |
| PflMI | CCANNNNNTGG | 2 cut(s) | 270, 555 |
| PkrI | GCNGC | 7 cut(s) | 173, 322, 325, 354, 561, 1017, 1219 |
| PleI | GAGTC | 3 cut(s) | 281, 904, 1462 |
| PluTI | GGCGCC | 2 cut(s) | 378, 549 |
| PpsI | GAGTC | 3 cut(s) | 281, 904, 1462 |
| PpuMI | RGGWCCY | 1 cut(s) | 794 |
| PshBI | ATTAAT | 1 cut(s) | 1146 |
| PsiI | TTATAA | 1 cut(s) | 1364 |
| Psp124BI | GAGCTC | 1 cut(s) | 683 |
| Psp5II | RGGWCCY | 1 cut(s) | 794 |
| Psp6I | CCWGG | 2 cut(s) | 161, 511 |
| PspFI | CCCAGC | 2 cut(s) | 1171, 1274 |
| PspGI | CCWGG | 2 cut(s) | 161, 511 |
| PspN4I | GGNNCC | 5 cut(s) | 376, 541, 547, 809, 1104 |
| PspPI | GGNCC | 4 cut(s) | 195, 794, 808, 874 |
| PspPPI | RGGWCCY | 1 cut(s) | 794 |
| PstNI | CAGNNNCTG | 2 cut(s) | 738, 1457 |
| PsuI | RGATCY | 2 cut(s) | 539, 1222 |
| RsaI | GTAC | 4 cut(s) | 382, 622, 1194, 1472 |
| RsaNI | GTAC | 4 cut(s) | 381, 621, 1193, 1471 |
| RseI | CAYNNNNRTG | 2 cut(s) | 924, 1513 |
| SacI | GAGCTC | 1 cut(s) | 683 |
| SaqAI | TTAA | 7 cut(s) | 6, 12, 63, 711, 1137, 1146, 1284 |
| SatI | GCNGC | 7 cut(s) | 172, 321, 324, 353, 560, 1016, 1218 |
| Sau3AI | GATC | 3 cut(s) | 539, 649, 1222 |
| Sau96I | GGNCC | 4 cut(s) | 195, 794, 808, 874 |
| SchI | GAGTC | 3 cut(s) | 281, 904, 1463 |
| ScrFI | CCNGG | 3 cut(s) | 163, 513, 767 |
| SduI | GDGCHC | 2 cut(s) | 118, 683 |
| SfaNI | GCATC | 3 cut(s) | 878, 943, 967 |
| SfoI | GGCGCC | 2 cut(s) | 376, 547 |
| SinI | GGWCC | 1 cut(s) | 794 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 924, 1513 |
| SsiI | CCGC | 6 cut(s) | 301, 321, 324, 460, 501, 1018 |
| SspDI | GGCGCC | 2 cut(s) | 374, 545 |
| SspMI | CTAG | 1 cut(s) | 629 |
| SstI | GAGCTC | 1 cut(s) | 683 |
| StyD4I | CCNGG | 3 cut(s) | 161, 511, 765 |
| TaaI | ACNGT | 4 cut(s) | 235, 740, 961, 1505 |
| TaiI | ACGT | 1 cut(s) | 1394 |
| TaqI | TCGA | 5 cut(s) | 330, 593, 655, 934, 1480 |
| TauI | GCSGC | 2 cut(s) | 323, 326 |
| TfiI | GAWTC | 4 cut(s) | 157, 534, 599, 1307 |
| Tru1I | TTAA | 7 cut(s) | 6, 12, 63, 711, 1137, 1146, 1284 |
| Tru9I | TTAA | 7 cut(s) | 6, 12, 63, 711, 1137, 1146, 1284 |
| TscAI | CASTG | 4 cut(s) | 95, 156, 1161, 1329 |
| TseI | GCWGC | 5 cut(s) | 171, 352, 559, 1015, 1217 |
| TspDTI | ATGAA | 5 cut(s) | 81, 852, 1359, 1370, 1553 |
| TspGWI | ACGGA | 3 cut(s) | 84, 1292, 1361 |
| TspRI | CASTG | 4 cut(s) | 95, 156, 1161, 1329 |
| Van91I | CCANNNNNTGG | 2 cut(s) | 270, 555 |
| VpaK11BI | GGWCC | 1 cut(s) | 794 |
| VspI | ATTAAT | 1 cut(s) | 1146 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 995 |
| XapI | RAATTY | 4 cut(s) | 704, 1331, 1366, 1540 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 1233 |
| XmnI | GAANNNNTTC | 2 cut(s) | 533, 1005 |
| XspI | CTAG | 1 cut(s) | 629 |