RLG00000024557

Hydrolyzes acetyl esters in homogalacturonan regions of pectin. In type I primary cell wall, galacturonic acid residues of pectin can be acetylated at the O-2 and O-3 positions. Decreasing the degree of acetylation of pectin gels in vitro alters their physical properties

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0001587
Physical Location & Seq
Forward (+)
36973632 .. 36978555
4924 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000024557

Sequence Viewer

Length: 1404 bp
ATGGCGGGGTCAACGGCGAACCTCAGAGTCCGCAGCCTTGTATGGTGGAGAAAGTGGGCCAGCAAGGAATGGGCGATCGCCACCCTCGCCTTTACCTTCATTGTCTTCCTTCTCACCCTCATCTCCAACTCTTGGAGCGACGAGCCTGGCTCCGCCGTCACCAACCCCTTCGATCCAACCACCGCCGCCGATTTCGTCGACTTGACTCTGCTGCACGACGCCGAACGCAGAGGCGCCCTTTGCTTAGATGGGACTGCTCCCGGGTACCATTTCCTAAAGGGGTTCGGATCCGGAGCCAAAAATTGGCTGCTTCACATTGAGGGTGGAGGTTGGTGCAATACAATAGAATCATGTTCTCTTCGGAAAACGACTCCATTAGGGTCTTCCAAGTTCATGGATCGTAGGGTTCAGTTTTCCGGGATTTTGAGCTCTCAGCCGTCAGAAAATCCTGACTTCTTTAACTGGAACAAAGTCAAAATACGGTACTGTGATGGCGCGTCTTTTGCTGGCCACCCCAAAAATGAGCCCAAGAACGGATCAAAACTTTTCTTTAGGGGACAGCTTATCTGGGAAGCACTTCTGGATGAACTCTTGTCAATTGGCTTGTCAAAGGCGAAACAGGCACTTCTTTCTGGATGCTCTGCCGGTGGGTTGGCAACTCTTATACACTGTGATGACTTTCAAGGTCTTCTACCTAGGGATTCAACTGTCAAATGTCTTGCTGATGCAGGTTTTTTTCTTGATGAGAAAGATGTTAACCAAAACCGGACCATGAGAACTTTTTATCGTGATGTTGTGGTTCTTCAGGATTTAGCAAAAAGTTTGCACAAGGATTGTGTTGCAAGAAAGGAACCAGCTAAGTGTCTCTTTCCCGAAGAAATTATTAAACACGTCAGCACTCCAGTGTTCCTTGTCAACCCAGCTTATGATTTTTGGCAGATACAACATATCTTGATACCTGAAGCGTCAGATCCACATGGCTACTGGAAAAAGTGCAAGCTCAACATCTATAATTGCAATCCTAGCCAGATTGAAACACTGCAAGGTTTCCGTCGTTCTATGCTAAATGCACTGAACGAATTCCAGAAAAATAAGGAGGGAGGAACTTTTATAAATTCATGTTTTATTCATTGCCAGACATGGTTTTCAGAGACATGGCATTCACCCAGTTCTCCACGAATAAACATTAAGACAATTGCAGAGTCTGTGGGTGATTGGTACTTTAATCGAAATGTTGCAAAGCAAATTGACTGCCCATTTCCTTGCAATCCCACCTGTTATCATATGAATTTCACTCAATTTTCACGAGAGCGTGTAGATACTGATAACAAAGGATCACCATCAGGAAAGGAAGAGATGAACAACTCTACTTCGGAGACGTCTTCTTCATTGGTGAAGTATTGA

Protein Analysis

468

Amino Acids

52.6

Weight (kDa)

8.08

Isoelectric Point (pI)

43.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PAE PF03283 63 - 426 3.5e-162 Pectinacetylesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1112
AatII GACGTC 1 cut(s) 1382
Acc36I ACCTGC 1 cut(s) 719
Acc65I GGTACC 1 cut(s) 264
AccB1I GGYRCC 2 cut(s) 233, 264
AccB7I CCANNNNNTGG 2 cut(s) 132, 303
AccI GTMKAC 1 cut(s) 198
AccII CGCG 1 cut(s) 497
AccIII TCCGGA 1 cut(s) 290
AciI CCGC 5 cut(s) 5, 31, 153, 183, 186
AclWI GGATC 7 cut(s) 167, 282, 295, 405, 544, 965, 1342
AcoI YGGCCR 1 cut(s) 508
AcsI RAATTY 3 cut(s) 1079, 1114, 1288
AcuI CTGAAG 2 cut(s) 788, 981
AcyI GRCGYC 3 cut(s) 219, 234, 1379
AfaI GTAC 3 cut(s) 266, 485, 1220
AfiI CCNNNNNNNGG 3 cut(s) 132, 303, 533
AflIII ACRYGT 1 cut(s) 889
AgsI TTSAA 3 cut(s) 683, 705, 1034
AjiI CACGTC 1 cut(s) 892
AjnI CCWGG 1 cut(s) 145
AleI CACNNNNGTG 1 cut(s) 902
AluBI AGCT 5 cut(s) 429, 562, 857, 923, 1000
AluI AGCT 5 cut(s) 429, 562, 857, 923, 1000
Alw21I GWGCWC 1 cut(s) 431
Alw26I GTCTC 3 cut(s) 869, 1145, 1370
AlwI GGATC 7 cut(s) 167, 282, 295, 405, 544, 965, 1342
AlwNI CAGNNNCTG 1 cut(s) 1205
Ama87I CYCGRG 1 cut(s) 260
Aor13HI TCCGGA 1 cut(s) 290
AoxI GGCC 2 cut(s) 57, 508
ApeKI GCWGC 3 cut(s) 33, 211, 307
ApoI RAATTY 3 cut(s) 1079, 1114, 1288
AsiSI GCGATCGC 1 cut(s) 78
Asp700I GAANNNNTTC 2 cut(s) 576, 1079
Asp718I GGTACC 1 cut(s) 264
AspA2I CCTAGG 1 cut(s) 695
AspLEI GCGC 2 cut(s) 236, 497
AspS9I GGNCC 2 cut(s) 57, 768
AsuC2I CCSGG 3 cut(s) 261, 262, 418
AsuHPI GGTGA 5 cut(s) 106, 151, 1155, 1223, 1329
AvaI CYCGRG 1 cut(s) 260
AvaII GGWCC 1 cut(s) 768
AvrII CCTAGG 1 cut(s) 695
BalI TGGCCA 1 cut(s) 510
BamHI GGATCC 1 cut(s) 287
BanI GGYRCC 2 cut(s) 233, 264
BanII GRGCYC 2 cut(s) 431, 528
BauI CACGAG 1 cut(s) 1305
BbsI GAAGAC 4 cut(s) 97, 375, 680, 1374
Bbv12I GWGCWC 1 cut(s) 431
BbvI GCAGC 3 cut(s) 45, 198, 294
BccI CCATC 3 cut(s) 242, 485, 1348
BceAI ACGGC 3 cut(s) 30, 140, 421
BciT130I CCWGG 1 cut(s) 147
BcnI CCSGG 3 cut(s) 261, 262, 418
BcoDI GTCTC 3 cut(s) 869, 1145, 1370
BfaI CTAG 2 cut(s) 696, 1023
BfoI RGCGCY 1 cut(s) 237
BfuAI ACCTGC 1 cut(s) 719
BisI GCNGC 4 cut(s) 34, 186, 212, 308
BlnI CCTAGG 1 cut(s) 695
BlsI GCNGC 4 cut(s) 35, 187, 213, 309
Bme1390I CCNGG 4 cut(s) 147, 261, 262, 418
Bme18I GGWCC 1 cut(s) 768
BmeT110I CYCGRG 1 cut(s) 260
BmgBI CACGTC 1 cut(s) 892
BmgT120I GGNCC 2 cut(s) 57, 768
BmiI GGNNCC 6 cut(s) 151, 235, 266, 289, 295, 852
BmrFI CCNGG 4 cut(s) 147, 261, 262, 418
BmrI ACTGGG 1 cut(s) 1161
BmsI GCATC 2 cut(s) 626, 715
BmuI ACTGGG 1 cut(s) 1161
BpiI GAAGAC 4 cut(s) 97, 375, 680, 1374
BplI GAGNNNNNCTC 2 cut(s) 134, 166
BpmI CTGGAG 1 cut(s) 885
BpuMI CCSGG 3 cut(s) 261, 262, 418
BsaBI GATNNNNATC 1 cut(s) 1339
BsaHI GRCGYC 3 cut(s) 219, 234, 1379
BsaJI CCNNGG 2 cut(s) 260, 695
BsaWI WCCGGW 2 cut(s) 290, 765
Bsc4I CCNNNNNNNGG 3 cut(s) 132, 303, 533
Bse118I RCCGGY 1 cut(s) 644
Bse1I ACTGG 4 cut(s) 467, 902, 989, 1167
Bse3DI GCAATG 1 cut(s) 1129
Bse8I GATNNNNATC 1 cut(s) 1339
BseAI TCCGGA 1 cut(s) 290
BseBI CCWGG 1 cut(s) 147
BseDI CCNNGG 2 cut(s) 260, 695
BseGI GGATG 2 cut(s) 589, 641
BseJI GATNNNNATC 1 cut(s) 1339
BseLI CCNNNNNNNGG 3 cut(s) 132, 303, 533
BseMI GCAATG 1 cut(s) 1129
BseMII CTCAG 2 cut(s) 37, 446
BseNI ACTGG 4 cut(s) 467, 902, 989, 1167
BseXI GCAGC 3 cut(s) 45, 198, 294
BseYI CCCAGC 1 cut(s) 919
BsgI GTGCAG 1 cut(s) 197
Bsh1236I CGCG 1 cut(s) 497
Bsh1285I CGRYCG 1 cut(s) 78
BshFI GGCC 2 cut(s) 59, 510
BshNI GGYRCC 2 cut(s) 233, 264
BsiEI CGRYCG 1 cut(s) 78
BsiHKAI GWGCWC 1 cut(s) 431
BsiHKCI CYCGRG 1 cut(s) 260
BsiSI CCGG 5 cut(s) 261, 291, 417, 645, 766
BslFI GGGAC 2 cut(s) 265, 570
BslI CCNNNNNNNGG 3 cut(s) 132, 303, 533
BsmAI GTCTC 3 cut(s) 869, 1145, 1370
BsmBI CGTCTC 1 cut(s) 1370
BsmFI GGGAC 2 cut(s) 265, 570
BsmI GAATGC 1 cut(s) 1159
BsnI GGCC 2 cut(s) 59, 510
BsoBI CYCGRG 1 cut(s) 260
Bsp1286I GDGCHC 2 cut(s) 431, 528
Bsp13I TCCGGA 1 cut(s) 290
Bsp143I GATC 7 cut(s) 75, 172, 287, 397, 536, 970, 1334
BspACI CCGC 5 cut(s) 5, 31, 153, 183, 186
BspANI GGCC 2 cut(s) 59, 510
BspCNI CTCAG 2 cut(s) 36, 445
BspEI TCCGGA 1 cut(s) 290
BspFNI CGCG 1 cut(s) 497
BspLI GGNNCC 6 cut(s) 151, 235, 266, 289, 295, 852
BspMI ACCTGC 1 cut(s) 719
BspPI GGATC 7 cut(s) 167, 282, 295, 405, 544, 965, 1342
BspT107I GGYRCC 2 cut(s) 233, 264
BsrDI GCAATG 1 cut(s) 1129
BsrFI RCCGGY 1 cut(s) 644
BsrI ACTGG 4 cut(s) 467, 902, 989, 1167
BssAI RCCGGY 1 cut(s) 644
BssECI CCNNGG 2 cut(s) 260, 695
BssMI GATC 7 cut(s) 75, 172, 287, 397, 536, 970, 1334
BssNI GRCGYC 3 cut(s) 219, 234, 1379
BssSI CACGAG 1 cut(s) 1305
BssT1I CCWWGG 1 cut(s) 695
Bst2BI CACGAG 1 cut(s) 1305
Bst2UI CCWGG 1 cut(s) 147
Bst4CI ACNGT 4 cut(s) 483, 488, 671, 709
Bst6I CTCTTC 2 cut(s) 363, 1347
BstACI GRCGYC 3 cut(s) 219, 234, 1379
BstC8I GCNNGC 3 cut(s) 61, 508, 998
BstDEI CTNAG 4 cut(s) 23, 244, 432, 858
BstF5I GGATG 2 cut(s) 589, 641
BstFNI CGCG 1 cut(s) 497
BstH2I RGCGCY 1 cut(s) 237
BstHHI GCGC 2 cut(s) 236, 497
BstKTI GATC 7 cut(s) 78, 175, 290, 400, 539, 973, 1337
BstMAI GTCTC 3 cut(s) 869, 1145, 1370
BstMBI GATC 7 cut(s) 75, 172, 287, 397, 536, 970, 1334
BstMCI CGRYCG 1 cut(s) 78
BstMWI GCNNNNNNNGC 5 cut(s) 86, 240, 503, 620, 1023
BstNI CCWGG 1 cut(s) 147
BstSCI CCNGG 4 cut(s) 145, 259, 260, 416
BstUI CGCG 1 cut(s) 497
BstV1I GCAGC 3 cut(s) 45, 198, 294
BstV2I GAAGAC 4 cut(s) 97, 375, 680, 1374
BstX2I RGATCY 2 cut(s) 287, 970
BstXI CCANNNNNNTGG 1 cut(s) 394
BstYI RGATCY 2 cut(s) 287, 970
BsuRI GGCC 2 cut(s) 59, 510
BtrI CACGTC 1 cut(s) 892
BtsCI GGATG 2 cut(s) 589, 641
BtsI GCAGTG 1 cut(s) 1037
BtsIMutI CAGTG 4 cut(s) 667, 909, 1037, 1070
BveI ACCTGC 1 cut(s) 719
Cac8I GCNNGC 3 cut(s) 61, 508, 998
CaiI CAGNNNCTG 1 cut(s) 1205
CfoI GCGC 2 cut(s) 236, 497
Cfr10I RCCGGY 1 cut(s) 644
Cfr13I GGNCC 2 cut(s) 57, 768
Cfr9I CCCGGG 1 cut(s) 260
CseI GACGC 3 cut(s) 227, 486, 954
Csp6I GTAC 3 cut(s) 265, 484, 1219
CviAII CATG 7 cut(s) 351, 394, 772, 977, 1119, 1140, 1155
CviQI GTAC 3 cut(s) 265, 484, 1219
DdeI CTNAG 4 cut(s) 23, 244, 432, 858
DinI GGCGCC 1 cut(s) 235
DpnI GATC 7 cut(s) 77, 174, 289, 399, 538, 972, 1336
DpnII GATC 7 cut(s) 75, 172, 287, 397, 536, 970, 1334
EaeI YGGCCR 1 cut(s) 508
Eam1104I CTCTTC 2 cut(s) 363, 1347
EarI CTCTTC 2 cut(s) 363, 1347
EciI GGCGGA 1 cut(s) 142
Ecl136II GAGCTC 1 cut(s) 429
Eco130I CCWWGG 1 cut(s) 695
Eco24I GRGCYC 2 cut(s) 431, 528
Eco47I GGWCC 1 cut(s) 768
Eco53kI GAGCTC 1 cut(s) 429
Eco57I CTGAAG 2 cut(s) 788, 981
Eco88I CYCGRG 1 cut(s) 260
EcoICRI GAGCTC 1 cut(s) 429
EcoRI GAATTC 1 cut(s) 1079
EcoRII CCWGG 1 cut(s) 145
EcoT14I CCWWGG 1 cut(s) 695
EcoT38I GRGCYC 2 cut(s) 431, 528
EgeI GGCGCC 1 cut(s) 235
EheI GGCGCC 1 cut(s) 235
ErhI CCWWGG 1 cut(s) 695
Esp3I CGTCTC 1 cut(s) 1370
FaeI CATG 7 cut(s) 354, 397, 775, 980, 1122, 1143, 1158
FalI AAGNNNNNCTT 2 cut(s) 851, 883
FaqI GGGAC 2 cut(s) 265, 570
FatI CATG 7 cut(s) 350, 393, 771, 976, 1118, 1139, 1154
FauNDI CATATG 1 cut(s) 1284
FblI GTMKAC 1 cut(s) 198
Fnu4HI GCNGC 4 cut(s) 34, 186, 212, 308
FokI GGATG 2 cut(s) 596, 648
FriOI GRGCYC 2 cut(s) 431, 528
Fsp4HI GCNGC 4 cut(s) 34, 186, 212, 308
FspBI CTAG 2 cut(s) 696, 1023
GlaI GCGC 2 cut(s) 235, 496
GluI GCNGC 4 cut(s) 34, 186, 212, 308
GsaI CCCAGC 1 cut(s) 923
GsuI CTGGAG 1 cut(s) 885
HaeII RGCGCY 1 cut(s) 237
HaeIII GGCC 2 cut(s) 59, 510
HapII CCGG 5 cut(s) 261, 291, 417, 645, 766
HgaI GACGC 3 cut(s) 227, 486, 954
HhaI GCGC 2 cut(s) 236, 497
Hin1I GRCGYC 3 cut(s) 219, 234, 1379
Hin1II CATG 7 cut(s) 354, 397, 775, 980, 1122, 1143, 1158
Hin6I GCGC 2 cut(s) 234, 495
HinP1I GCGC 2 cut(s) 234, 495
HincII GTYRAC 4 cut(s) 12, 199, 757, 916
HindII GTYRAC 4 cut(s) 12, 199, 757, 916
HinfI GANTC 6 cut(s) 27, 205, 347, 370, 701, 1202
HpaI GTTAAC 1 cut(s) 757
HpaII CCGG 5 cut(s) 261, 291, 417, 645, 766
HphI GGTGA 5 cut(s) 106, 151, 1155, 1223, 1329
Hpy166II GTNNAC 4 cut(s) 12, 199, 757, 916
Hpy188I TCNGA 7 cut(s) 26, 287, 363, 442, 970, 1150, 1375
Hpy8I GTNNAC 4 cut(s) 12, 199, 757, 916
Hpy99I CGWCG 4 cut(s) 143, 200, 221, 1056
HpyAV CCTTC 3 cut(s) 106, 119, 178
HpyCH4III ACNGT 4 cut(s) 483, 488, 671, 709
HpyCH4IV ACGT 2 cut(s) 891, 1379
HpyF10VI GCNNNNNNNGC 5 cut(s) 86, 240, 503, 620, 1023
HpyF3I CTNAG 4 cut(s) 23, 244, 432, 858
HpySE526I ACGT 2 cut(s) 891, 1379
Hsp92I GRCGYC 3 cut(s) 219, 234, 1379
Hsp92II CATG 7 cut(s) 354, 397, 775, 980, 1122, 1143, 1158
HspAI GCGC 2 cut(s) 234, 495
KasI GGCGCC 1 cut(s) 233
Kpn2I TCCGGA 1 cut(s) 290
KpnI GGTACC 1 cut(s) 268
KspAI GTTAAC 1 cut(s) 757
Kzo9I GATC 7 cut(s) 75, 172, 287, 397, 536, 970, 1334
LmnI GCTCC 4 cut(s) 135, 155, 262, 293
Lsp1109I GCAGC 3 cut(s) 45, 198, 294
LweI GCATC 2 cut(s) 626, 715
MaeI CTAG 2 cut(s) 696, 1023
MaeII ACGT 2 cut(s) 891, 1379
MaeIII GTNAC 1 cut(s) 157
MalI GATC 7 cut(s) 77, 174, 289, 399, 538, 972, 1336
MboI GATC 7 cut(s) 75, 172, 287, 397, 536, 970, 1334
MboII GAAGA 9 cut(s) 97, 350, 375, 680, 794, 887, 1364, 1374, 1377
MfeI CAATTG 2 cut(s) 597, 1194
MflI RGATCY 2 cut(s) 287, 970
MhlI GDGCHC 2 cut(s) 431, 528
MlsI TGGCCA 1 cut(s) 510
MluNI TGGCCA 1 cut(s) 510
Mly113I GGCGCC 1 cut(s) 234
MlyI GAGTC 4 cut(s) 36, 199, 364, 1211
MmeI TCCRAC 2 cut(s) 150, 200
MnlI CCTC 8 cut(s) 32, 95, 128, 224, 313, 320, 1090, 1094
Mox20I TGGCCA 1 cut(s) 510
MroI TCCGGA 1 cut(s) 290
MroXI GAANNNNTTC 2 cut(s) 576, 1079
MscI TGGCCA 1 cut(s) 510
MseI TTAA 5 cut(s) 459, 756, 885, 1188, 1224
MslI CAYNNNNRTG 2 cut(s) 672, 902
Msp20I TGGCCA 1 cut(s) 510
MspI CCGG 5 cut(s) 261, 291, 417, 645, 766
MspR9I CCNGG 4 cut(s) 147, 261, 262, 418
MunI CAATTG 2 cut(s) 597, 1194
Mva1269I GAATGC 1 cut(s) 1159
MvaI CCWGG 1 cut(s) 147
MvnI CGCG 1 cut(s) 497
MwoI GCNNNNNNNGC 5 cut(s) 86, 240, 503, 620, 1023
NarI GGCGCC 1 cut(s) 234
NciI CCSGG 3 cut(s) 261, 262, 418
NdeI CATATG 1 cut(s) 1284
NdeII GATC 7 cut(s) 75, 172, 287, 397, 536, 970, 1334
NlaIII CATG 7 cut(s) 354, 397, 775, 980, 1122, 1143, 1158
NlaIV GGNNCC 6 cut(s) 151, 235, 266, 289, 295, 852
NmuCI GTSAC 1 cut(s) 157
OliI CACNNNNGTG 1 cut(s) 902
PctI GAATGC 1 cut(s) 1159
PdmI GAANNNNTTC 2 cut(s) 576, 1079
PfeI GAWTC 2 cut(s) 347, 701
PflMI CCANNNNNTGG 2 cut(s) 132, 303
PfoI TCCNGGA 1 cut(s) 416
PkrI GCNGC 4 cut(s) 35, 187, 213, 309
Ple19I CGATCG 1 cut(s) 78
PleI GAGTC 4 cut(s) 35, 199, 364, 1210
PluTI GGCGCC 1 cut(s) 237
PpsI GAGTC 4 cut(s) 35, 199, 364, 1210
PsiI TTATAA 1 cut(s) 1112
Psp124BI GAGCTC 1 cut(s) 431
Psp6I CCWGG 1 cut(s) 145
PspFI CCCAGC 1 cut(s) 919
PspGI CCWGG 1 cut(s) 145
PspN4I GGNNCC 6 cut(s) 151, 235, 266, 289, 295, 852
PspPI GGNCC 2 cut(s) 57, 768
PstNI CAGNNNCTG 1 cut(s) 1205
PsuI RGATCY 2 cut(s) 287, 970
PvuI CGATCG 1 cut(s) 78
RgaI GCGATCGC 1 cut(s) 78
RsaI GTAC 3 cut(s) 266, 485, 1220
RsaNI GTAC 3 cut(s) 265, 484, 1219
RseI CAYNNNNRTG 2 cut(s) 672, 902
SacI GAGCTC 1 cut(s) 431
SalI GTCGAC 1 cut(s) 197
SaqAI TTAA 5 cut(s) 459, 756, 885, 1188, 1224
SatI GCNGC 4 cut(s) 34, 186, 212, 308
Sau3AI GATC 7 cut(s) 75, 172, 287, 397, 536, 970, 1334
Sau96I GGNCC 2 cut(s) 57, 768
SchI GAGTC 4 cut(s) 36, 199, 364, 1211
ScrFI CCNGG 4 cut(s) 147, 261, 262, 418
SduI GDGCHC 2 cut(s) 431, 528
SfaAI GCGATCGC 1 cut(s) 78
SfaNI GCATC 2 cut(s) 626, 715
SfoI GGCGCC 1 cut(s) 235
SgfI GCGATCGC 1 cut(s) 78
SinI GGWCC 1 cut(s) 768
SmaI CCCGGG 1 cut(s) 262
SmiMI CAYNNNNRTG 2 cut(s) 672, 902
SsiI CCGC 5 cut(s) 5, 31, 153, 183, 186
SspDI GGCGCC 1 cut(s) 233
SspMI CTAG 2 cut(s) 696, 1023
SstI GAGCTC 1 cut(s) 431
StyD4I CCNGG 4 cut(s) 145, 259, 260, 416
StyI CCWWGG 1 cut(s) 695
TaaI ACNGT 4 cut(s) 483, 488, 671, 709
TaiI ACGT 2 cut(s) 894, 1382
TaqI TCGA 3 cut(s) 171, 198, 1228
TauI GCSGC 1 cut(s) 188
TfiI GAWTC 2 cut(s) 347, 701
Tru1I TTAA 5 cut(s) 459, 756, 885, 1188, 1224
Tru9I TTAA 5 cut(s) 459, 756, 885, 1188, 1224
TscAI CASTG 4 cut(s) 674, 909, 1044, 1077
TseFI GTSAC 1 cut(s) 157
TseI GCWGC 3 cut(s) 33, 211, 307
Tsp45I GTSAC 1 cut(s) 157
TspDTI ATGAA 8 cut(s) 88, 382, 600, 1107, 1118, 1301, 1373, 1377
TspGWI ACGGA 2 cut(s) 549, 1040
TspMI CCCGGG 1 cut(s) 260
TspRI CASTG 4 cut(s) 674, 909, 1044, 1077
Van91I CCANNNNNTGG 2 cut(s) 132, 303
VpaK11BI GGWCC 1 cut(s) 768
XapI RAATTY 3 cut(s) 1079, 1114, 1288
XcmI CCANNNNNNNNNTGG 1 cut(s) 981
XmaI CCCGGG 1 cut(s) 260
XmaJI CCTAGG 1 cut(s) 695
XmiI GTMKAC 1 cut(s) 198
XmnI GAANNNNTTC 2 cut(s) 576, 1079
XspI CTAG 2 cut(s) 696, 1023
ZraI GACGTC 1 cut(s) 1380
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.