pycom04g11450

Hydrolyzes acetyl esters in homogalacturonan regions of pectin. In type I primary cell wall, galacturonic acid residues of pectin can be acetylated at the O-2 and O-3 positions. Decreasing the degree of acetylation of pectin gels in vitro alters their physical properties

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr4
OG0001587
Physical Location & Seq
Forward (+)
14304327 .. 14307726
3400 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom04g11450.12

Sequence Viewer

Length: 1332 bp
ATGGTGGTGGAGAGAGTCGGGTCAAATTCTATGGCGAATATTTCTGGGCTTCGCGGCCCGCTGTGGTGGATGCAGTGGGCCAAGCGTGGCTGGGCAATCGCCGCCGTCGGATTCGCCGTCTTCGTCTTCGTCTTCGCTCTCTCTGTCGCCTCCTACTATCGGAGTTCCGAGCCGGAGTCCAACATCGCGAACTCATTTCATCGCATCACCACCTCCGATTTGATCGATCTGACATTGTTGCACAGCGCCAAAGATAGAGGCGCCCTTTGTTTAGATGGAAGTTTGCCTGGCTACCATTTTCAAAAGGGATTTGGATCCGGCGCCAAAAATTGGCTACTTTTCATTGAGGGTGGAGGTTGGTGCAACACAATAGAATCATGTATTTTGCGTAAACATATGCATTTAGGATCTTCCAAACATATGGGACGTCGAGTTCACTTTACTGGGATTTTAAGCCCTTACCCTGACCATAATCCTGGATTCTTTAACTGGAACCGAGTCAGAATACAGTATTGTGATGGTGCATTTATAGCCGGTCACCCTGATAATGAGTTGAAGAATGGAACGGAACTTTTCTTTAGGGGCCGGCTCATCTGGGAAGCAATTATGGATGAACTCTTGTCATTTGGCTTATCAAAAGCGGAACAGGCCCTTCTTTCGGGATGCTCTTCCGGTGGGTTGGCAGCTCTTATACATTGTGATGAATTTCGACATGTTTTGCCTAAGGATGCCACTGTCAAATGTCTAGGCGATGCGGGTTTTTTTCTTGATGAGAAAGATGTTCTCCACAATCCTACCGTGAGATCTCTCTTTCGTGATGTTGTCAACCTTCAGGGGTTAGCGAAAAGTTTGAACAAGGATTGTGTTGCAAGAATGGAACCAGATAGGTGTATCTTTCCTGAAGAAATTGTTAAAAACATCAAAACCCCCGTGTTCCTCGTTCAATCAGCTTATGATTTTTGGCAAATACAAAATACCTTGATACCTAAAGCATCTGATCCACATAGCTACTGGGAAAAGTGCAGACTGAACATTTATAATTGCAATTCTACCCAGGTTGAAATACTTCTAGGTTTCAGGGGATCTCTGCTGAAGGCGTTGAATGATTTCGAGAAAAATAAGGAAGGAGGAATGTTTATAAGTTCTTGCTTTATTCATTGCCAGACATTGATGACTGAGATATGGAATTCGCCGAAATCTTTAAGGATAAACAACAAGACAATTGCAGAGTCTGTGGGGGACTGGTACTTCAATCGAAGTGCAGCGAAGCTAATAGACTGCCCGTTTCCATGCAATCCGACCTGCTACAATGTGAATTTCACTCGAGGTTGA

Protein Analysis

444

Amino Acids

49.83

Weight (kDa)

8.27

Isoelectric Point (pI)

41.69

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1038, 1139
AatII GACGTC 1 cut(s) 430
Acc36I ACCTGC 1 cut(s) 1310
AccB1I GGYRCC 2 cut(s) 260, 320
AccB7I CCANNNNNTGG 1 cut(s) 330
AccII CGCG 2 cut(s) 54, 188
AciI CCGC 5 cut(s) 54, 59, 102, 641, 755
AclWI GGATC 5 cut(s) 309, 322, 415, 992, 1090
AcsI RAATTY 4 cut(s) 25, 704, 1186, 1315
AcuI CTGAAG 3 cut(s) 815, 921, 1112
AcyI GRCGYC 3 cut(s) 261, 321, 427
AfaI GTAC 1 cut(s) 1247
AfiI CCNNNNNNNGG 3 cut(s) 159, 330, 658
AflIII ACRYGT 1 cut(s) 712
AgsI TTSAA 7 cut(s) 302, 556, 853, 944, 1061, 1102, 1252
AjnI CCWGG 3 cut(s) 286, 475, 1053
AloI GAACNNNNNNTCC 2 cut(s) 417, 449
AluBI AGCT 4 cut(s) 686, 950, 1008, 1270
AluI AGCT 4 cut(s) 686, 950, 1008, 1270
AlwI GGATC 5 cut(s) 309, 322, 415, 992, 1090
AlwNI CAGNNNCTG 1 cut(s) 1232
Ama87I CYCGRG 1 cut(s) 1323
AoxI GGCC 4 cut(s) 55, 78, 583, 648
ApeKI GCWGC 2 cut(s) 683, 1262
ApoI RAATTY 4 cut(s) 25, 704, 1186, 1315
Asp700I GAANNNNTTC 2 cut(s) 1065, 1106
AspLEI GCGC 3 cut(s) 248, 263, 323
AspS9I GGNCC 4 cut(s) 56, 78, 583, 649
AsuHPI GGTGA 2 cut(s) 199, 530
AvaI CYCGRG 1 cut(s) 1323
AxyI CCTNAGG 1 cut(s) 723
BamHI GGATCC 1 cut(s) 314
BanI GGYRCC 2 cut(s) 260, 320
BbsI GAAGAC 3 cut(s) 112, 118, 124
BbvI GCAGC 2 cut(s) 695, 1274
BccI CCATC 2 cut(s) 269, 512
BceAI ACGGC 2 cut(s) 89, 101
BciT130I CCWGG 3 cut(s) 288, 477, 1055
BfaI CTAG 2 cut(s) 746, 1070
BfoI RGCGCY 3 cut(s) 249, 264, 324
BfuAI ACCTGC 1 cut(s) 1310
BglII AGATCT 1 cut(s) 803
BisI GCNGC 4 cut(s) 55, 102, 684, 1263
BlsI GCNGC 4 cut(s) 56, 103, 685, 1264
Bme1390I CCNGG 3 cut(s) 288, 477, 1055
BmeT110I CYCGRG 1 cut(s) 1323
BmgT120I GGNCC 4 cut(s) 56, 78, 583, 649
BmiI GGNNCC 6 cut(s) 262, 316, 322, 494, 584, 879
BmrFI CCNGG 3 cut(s) 288, 477, 1055
BmrI ACTGGG 2 cut(s) 453, 1021
BmsI GCATC 6 cut(s) 60, 213, 653, 718, 742, 1001
BmuI ACTGGG 2 cut(s) 453, 1021
BpiI GAAGAC 3 cut(s) 112, 118, 124
Bsa29I ATCGAT 1 cut(s) 225
BsaBI GATNNNNATC 1 cut(s) 313
BsaHI GRCGYC 3 cut(s) 261, 321, 427
BsaJI CCNNGG 1 cut(s) 1053
BsaWI WCCGGW 1 cut(s) 671
Bsc4I CCNNNNNNNGG 3 cut(s) 159, 330, 658
Bse118I RCCGGY 2 cut(s) 533, 585
Bse1I ACTGG 4 cut(s) 448, 494, 1016, 1247
Bse21I CCTNAGG 1 cut(s) 723
Bse3DI GCAATG 1 cut(s) 1156
Bse8I GATNNNNATC 1 cut(s) 313
BseBI CCWGG 3 cut(s) 288, 477, 1055
BseCI ATCGAT 1 cut(s) 225
BseDI CCNNGG 1 cut(s) 1053
BseGI GGATG 4 cut(s) 75, 616, 668, 733
BseJI GATNNNNATC 1 cut(s) 313
BseLI CCNNNNNNNGG 3 cut(s) 159, 330, 658
BseMI GCAATG 1 cut(s) 1156
BseMII CTCAG 1 cut(s) 1167
BseNI ACTGG 4 cut(s) 448, 494, 1016, 1247
BseXI GCAGC 2 cut(s) 695, 1274
BseYI CCCAGC 1 cut(s) 90
BsgI GTGCAG 2 cut(s) 1042, 1281
Bsh1236I CGCG 2 cut(s) 54, 188
BshFI GGCC 4 cut(s) 57, 80, 585, 650
BshNI GGYRCC 2 cut(s) 260, 320
BshVI ATCGAT 1 cut(s) 225
BsiHKCI CYCGRG 1 cut(s) 1323
BsiSI CCGG 5 cut(s) 173, 318, 534, 586, 672
BslFI GGGAC 2 cut(s) 438, 1253
BslI CCNNNNNNNGG 3 cut(s) 159, 330, 658
BsmFI GGGAC 2 cut(s) 438, 1253
BsnI GGCC 4 cut(s) 57, 80, 585, 650
BsoBI CYCGRG 1 cut(s) 1323
Bsp143I GATC 7 cut(s) 222, 226, 314, 407, 803, 997, 1082
Bsp68I TCGCGA 1 cut(s) 188
BspACI CCGC 5 cut(s) 54, 59, 102, 641, 755
BspANI GGCC 4 cut(s) 57, 80, 585, 650
BspCNI CTCAG 1 cut(s) 1168
BspDI ATCGAT 1 cut(s) 225
BspFNI CGCG 2 cut(s) 54, 188
BspLI GGNNCC 6 cut(s) 262, 316, 322, 494, 584, 879
BspMI ACCTGC 1 cut(s) 1310
BspPI GGATC 5 cut(s) 309, 322, 415, 992, 1090
BspQI GCTCTTC 1 cut(s) 673
BspT107I GGYRCC 2 cut(s) 260, 320
BsrDI GCAATG 1 cut(s) 1156
BsrFI RCCGGY 2 cut(s) 533, 585
BsrI ACTGG 4 cut(s) 448, 494, 1016, 1247
BssAI RCCGGY 2 cut(s) 533, 585
BssECI CCNNGG 1 cut(s) 1053
BssMI GATC 7 cut(s) 222, 226, 314, 407, 803, 997, 1082
BssNI GRCGYC 3 cut(s) 261, 321, 427
Bst2UI CCWGG 3 cut(s) 288, 477, 1055
Bst4CI ACNGT 3 cut(s) 510, 736, 799
Bst6I CTCTTC 1 cut(s) 673
BstACI GRCGYC 3 cut(s) 261, 321, 427
BstC8I GCNNGC 2 cut(s) 59, 587
BstDEI CTNAG 2 cut(s) 723, 1176
BstEII GGTNACC 1 cut(s) 536
BstF5I GGATG 4 cut(s) 75, 616, 668, 733
BstFNI CGCG 2 cut(s) 54, 188
BstH2I RGCGCY 3 cut(s) 249, 264, 324
BstHHI GCGC 3 cut(s) 248, 263, 323
BstKTI GATC 7 cut(s) 225, 229, 317, 410, 806, 1000, 1085
BstMBI GATC 7 cut(s) 222, 226, 314, 407, 803, 997, 1082
BstMWI GCNNNNNNNGC 3 cut(s) 101, 530, 647
BstNI CCWGG 3 cut(s) 288, 477, 1055
BstNSI RCATGY 1 cut(s) 716
BstPI GGTNACC 1 cut(s) 536
BstSCI CCNGG 3 cut(s) 286, 475, 1053
BstUI CGCG 2 cut(s) 54, 188
BstV1I GCAGC 2 cut(s) 695, 1274
BstV2I GAAGAC 3 cut(s) 112, 118, 124
BstX2I RGATCY 4 cut(s) 314, 407, 803, 1082
BstXI CCANNNNNNTGG 2 cut(s) 421, 476
BstYI RGATCY 4 cut(s) 314, 407, 803, 1082
Bsu15I ATCGAT 1 cut(s) 225
Bsu36I CCTNAGG 1 cut(s) 723
BsuRI GGCC 4 cut(s) 57, 80, 585, 650
BsuTUI ATCGAT 1 cut(s) 225
BtgZI GCGATG 3 cut(s) 169, 185, 765
BtsCI GGATG 4 cut(s) 75, 616, 668, 733
BtsI GCAGTG 1 cut(s) 80
BtsIMutI CAGTG 2 cut(s) 80, 732
BtuMI TCGCGA 1 cut(s) 188
BveI ACCTGC 1 cut(s) 1310
Cac8I GCNNGC 2 cut(s) 59, 587
CaiI CAGNNNCTG 1 cut(s) 1232
CfoI GCGC 3 cut(s) 248, 263, 323
Cfr10I RCCGGY 2 cut(s) 533, 585
Cfr13I GGNCC 4 cut(s) 56, 78, 583, 649
ClaI ATCGAT 1 cut(s) 225
Csp6I GTAC 1 cut(s) 1246
CviAII CATG 3 cut(s) 378, 713, 1290
CviQI GTAC 1 cut(s) 1246
DdeI CTNAG 2 cut(s) 723, 1176
DinI GGCGCC 2 cut(s) 262, 322
DpnI GATC 7 cut(s) 224, 228, 316, 409, 805, 999, 1084
DpnII GATC 7 cut(s) 222, 226, 314, 407, 803, 997, 1082
Eam1104I CTCTTC 1 cut(s) 673
EarI CTCTTC 1 cut(s) 673
Eco57I CTGAAG 3 cut(s) 815, 921, 1112
Eco81I CCTNAGG 1 cut(s) 723
Eco88I CYCGRG 1 cut(s) 1323
Eco91I GGTNACC 1 cut(s) 536
EcoO109I RGGNCCY 1 cut(s) 649
EcoO65I GGTNACC 1 cut(s) 536
EcoRI GAATTC 1 cut(s) 1186
EcoRII CCWGG 3 cut(s) 286, 475, 1053
EcoT22I ATGCAT 1 cut(s) 402
EgeI GGCGCC 2 cut(s) 262, 322
EheI GGCGCC 2 cut(s) 262, 322
FaeI CATG 3 cut(s) 381, 716, 1293
FaqI GGGAC 2 cut(s) 438, 1253
FatI CATG 3 cut(s) 377, 712, 1289
FauI CCCGC 2 cut(s) 66, 748
FauNDI CATATG 2 cut(s) 396, 420
Fnu4HI GCNGC 4 cut(s) 55, 102, 684, 1263
FokI GGATG 4 cut(s) 82, 623, 675, 740
Fsp4HI GCNGC 4 cut(s) 55, 102, 684, 1263
FspBI CTAG 2 cut(s) 746, 1070
GlaI GCGC 3 cut(s) 247, 262, 322
GluI GCNGC 4 cut(s) 55, 102, 684, 1263
GsaI CCCAGC 1 cut(s) 94
HaeII RGCGCY 3 cut(s) 249, 264, 324
HaeIII GGCC 4 cut(s) 57, 80, 585, 650
HapII CCGG 5 cut(s) 173, 318, 534, 586, 672
HhaI GCGC 3 cut(s) 248, 263, 323
Hin1I GRCGYC 3 cut(s) 261, 321, 427
Hin1II CATG 3 cut(s) 381, 716, 1293
Hin6I GCGC 3 cut(s) 246, 261, 321
HinP1I GCGC 3 cut(s) 246, 261, 321
HincII GTYRAC 1 cut(s) 826
HindII GTYRAC 1 cut(s) 826
HinfI GANTC 7 cut(s) 15, 111, 176, 374, 480, 498, 1229
HpaII CCGG 5 cut(s) 173, 318, 534, 586, 672
HphI GGTGA 2 cut(s) 199, 530
Hpy166II GTNNAC 3 cut(s) 392, 436, 826
Hpy188I TCNGA 8 cut(s) 110, 162, 169, 217, 231, 503, 997, 1299
Hpy188III TCNNGA 6 cut(s) 187, 660, 767, 815, 899, 1111
Hpy8I GTNNAC 3 cut(s) 392, 436, 826
Hpy99I CGWCG 2 cut(s) 110, 432
HpyAV CCTTC 4 cut(s) 662, 839, 1087, 1118
HpyCH4III ACNGT 3 cut(s) 510, 736, 799
HpyCH4IV ACGT 1 cut(s) 427
HpyF10VI GCNNNNNNNGC 3 cut(s) 101, 530, 647
HpyF3I CTNAG 2 cut(s) 723, 1176
HpySE526I ACGT 1 cut(s) 427
Hsp92I GRCGYC 3 cut(s) 261, 321, 427
Hsp92II CATG 3 cut(s) 381, 716, 1293
HspAI GCGC 3 cut(s) 246, 261, 321
KasI GGCGCC 2 cut(s) 260, 320
KroI GCCGGC 1 cut(s) 585
KroNI GCCGGC 1 cut(s) 587
Kzo9I GATC 7 cut(s) 222, 226, 314, 407, 803, 997, 1082
LguI GCTCTTC 1 cut(s) 673
Lsp1109I GCAGC 2 cut(s) 695, 1274
LweI GCATC 6 cut(s) 60, 213, 653, 718, 742, 1001
MaeI CTAG 2 cut(s) 746, 1070
MaeII ACGT 1 cut(s) 427
MaeIII GTNAC 1 cut(s) 536
MalI GATC 7 cut(s) 224, 228, 316, 409, 805, 999, 1084
MboI GATC 7 cut(s) 222, 226, 314, 407, 803, 997, 1082
MboII GAAGA 7 cut(s) 112, 118, 124, 402, 568, 660, 914
MfeI CAATTG 1 cut(s) 1221
MflI RGATCY 4 cut(s) 314, 407, 803, 1082
Mly113I GGCGCC 2 cut(s) 261, 321
MlyI GAGTC 4 cut(s) 24, 185, 507, 1238
MmeI TCCRAC 3 cut(s) 88, 204, 1322
MnlI CCTC 8 cut(s) 160, 223, 251, 340, 347, 947, 1121, 1319
Mph1103I ATGCAT 1 cut(s) 402
MroNI GCCGGC 1 cut(s) 585
MroXI GAANNNNTTC 2 cut(s) 1065, 1106
MseI TTAA 4 cut(s) 452, 486, 912, 1202
MslI CAYNNNNRTG 1 cut(s) 699
MspA1I CMGCKG 1 cut(s) 61
MspI CCGG 5 cut(s) 173, 318, 534, 586, 672
MspR9I CCNGG 3 cut(s) 288, 477, 1055
MunI CAATTG 1 cut(s) 1221
MvaI CCWGG 3 cut(s) 288, 477, 1055
MvnI CGCG 2 cut(s) 54, 188
MwoI GCNNNNNNNGC 3 cut(s) 101, 530, 647
NaeI GCCGGC 1 cut(s) 587
NarI GGCGCC 2 cut(s) 261, 321
NdeI CATATG 2 cut(s) 396, 420
NdeII GATC 7 cut(s) 222, 226, 314, 407, 803, 997, 1082
NgoMIV GCCGGC 1 cut(s) 585
NlaIII CATG 3 cut(s) 381, 716, 1293
NlaIV GGNNCC 6 cut(s) 262, 316, 322, 494, 584, 879
NmuCI GTSAC 1 cut(s) 536
NruI TCGCGA 1 cut(s) 188
NsiI ATGCAT 1 cut(s) 402
NspI RCATGY 1 cut(s) 716
PaeR7I CTCGAG 1 cut(s) 1323
PciI ACATGT 1 cut(s) 712
PciSI GCTCTTC 1 cut(s) 673
PcsI WCGNNNNNNNCGW 2 cut(s) 114, 120
PdiI GCCGGC 1 cut(s) 587
PdmI GAANNNNTTC 2 cut(s) 1065, 1106
PfeI GAWTC 3 cut(s) 111, 374, 480
PflMI CCANNNNNTGG 1 cut(s) 330
PfoI TCCNGGA 1 cut(s) 475
PkrI GCNGC 4 cut(s) 56, 103, 685, 1264
PleI GAGTC 4 cut(s) 23, 184, 506, 1237
PluTI GGCGCC 2 cut(s) 264, 324
PpsI GAGTC 4 cut(s) 23, 184, 506, 1237
PscI ACATGT 1 cut(s) 712
PsiI TTATAA 2 cut(s) 1038, 1139
Psp6I CCWGG 3 cut(s) 286, 475, 1053
PspEI GGTNACC 1 cut(s) 536
PspFI CCCAGC 1 cut(s) 90
PspGI CCWGG 3 cut(s) 286, 475, 1053
PspN4I GGNNCC 6 cut(s) 262, 316, 322, 494, 584, 879
PspPI GGNCC 4 cut(s) 56, 78, 583, 649
PspXI VCTCGAGB 1 cut(s) 1323
PstNI CAGNNNCTG 1 cut(s) 1232
PsuI RGATCY 4 cut(s) 314, 407, 803, 1082
RruI TCGCGA 1 cut(s) 188
RsaI GTAC 1 cut(s) 1247
RsaNI GTAC 1 cut(s) 1246
RseI CAYNNNNRTG 1 cut(s) 699
SapI GCTCTTC 1 cut(s) 673
SaqAI TTAA 4 cut(s) 452, 486, 912, 1202
SatI GCNGC 4 cut(s) 55, 102, 684, 1263
Sau3AI GATC 7 cut(s) 222, 226, 314, 407, 803, 997, 1082
Sau96I GGNCC 4 cut(s) 56, 78, 583, 649
SchI GAGTC 4 cut(s) 24, 185, 507, 1238
ScrFI CCNGG 3 cut(s) 288, 477, 1055
SfaNI GCATC 6 cut(s) 60, 213, 653, 718, 742, 1001
SfoI GGCGCC 2 cut(s) 262, 322
Sfr274I CTCGAG 1 cut(s) 1323
SlaI CTCGAG 1 cut(s) 1323
SmiMI CAYNNNNRTG 1 cut(s) 699
SmlI CTYRAG 1 cut(s) 1323
SmoI CTYRAG 1 cut(s) 1323
SsiI CCGC 5 cut(s) 54, 59, 102, 641, 755
SspDI GGCGCC 2 cut(s) 260, 320
SspI AATATT 1 cut(s) 40
SspMI CTAG 2 cut(s) 746, 1070
StyD4I CCNGG 3 cut(s) 286, 475, 1053
TaaI ACNGT 3 cut(s) 510, 736, 799
TaiI ACGT 1 cut(s) 430
TaqI TCGA 6 cut(s) 225, 430, 709, 1110, 1255, 1324
TauI GCSGC 2 cut(s) 57, 104
TfiI GAWTC 3 cut(s) 111, 374, 480
Tru1I TTAA 4 cut(s) 452, 486, 912, 1202
Tru9I TTAA 4 cut(s) 452, 486, 912, 1202
TscAI CASTG 2 cut(s) 80, 739
TseFI GTSAC 1 cut(s) 536
TseI GCWGC 2 cut(s) 683, 1262
Tsp45I GTSAC 1 cut(s) 536
TspDTI ATGAA 5 cut(s) 188, 331, 627, 717, 1145
TspGWI ACGGA 1 cut(s) 581
TspRI CASTG 2 cut(s) 80, 739
Van91I CCANNNNNTGG 1 cut(s) 330
XapI RAATTY 4 cut(s) 25, 704, 1186, 1315
XceI RCATGY 1 cut(s) 716
XcmI CCANNNNNNNNNTGG 1 cut(s) 1008
XhoI CTCGAG 1 cut(s) 1323
XmnI GAANNNNTTC 2 cut(s) 1065, 1106
XspI CTAG 2 cut(s) 746, 1070
ZraI GACGTC 1 cut(s) 428
Zsp2I ATGCAT 1 cut(s) 402
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.