Rh3AG130200

Hydrolyzes acetyl esters in homogalacturonan regions of pectin. In type I primary cell wall, galacturonic acid residues of pectin can be acetylated at the O-2 and O-3 positions. Decreasing the degree of acetylation of pectin gels in vitro alters their physical properties

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3A
OG0001587
Physical Location & Seq
Reverse (-)
11267788 .. 11284192
16405 bp
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UTR
Exon/CDS
Intron
Rh3AG130200.1

Sequence Viewer

Length: 315 bp
ATGGATGAACTCTTGTCAATTGGCTTGTCAAAGTCAAAACAGGCGCTTCTTTCTGGATGCTCAGCCGGCGGGTTGGCAACTCTTATACACTGTGATGACTTTCGAGGTCTTCTACCTAGGGATTCAAGGATTCAACTGTCAAATGTCATGCCGATGCAGGATTTAGCAAAAAGTTTGCACAAGGATTCTGTTGCAAGAAAGGAACCAGCTAAGGCAAGGCAGCTTGTAAAGCTTCTCCTACAAATGTCTGGCATTTCATTTTTACTAGATTTAGACCCTAGATTGATTGCCACACAGACTTCTTTCCATTTGAGG

Protein Analysis

105

Amino Acids

11.54

Weight (kDa)

9.44

Isoelectric Point (pI)

39.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PAE PF03283 1 - 45 3.4e-14 Pectinacetylesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 69
AgsI TTSAA 2 cut(s) 126, 134
AluBI AGCT 3 cut(s) 209, 223, 232
AluI AGCT 3 cut(s) 209, 223, 232
ApeKI GCWGC 1 cut(s) 220
AspA2I CCTAGG 1 cut(s) 116
AspLEI GCGC 1 cut(s) 46
AvrII CCTAGG 1 cut(s) 116
BbsI GAAGAC 1 cut(s) 101
BbvI GCAGC 1 cut(s) 232
BfaI CTAG 3 cut(s) 117, 266, 279
BfoI RGCGCY 1 cut(s) 47
BisI GCNGC 1 cut(s) 221
BlnI CCTAGG 1 cut(s) 116
BlpI GCTNAGC 1 cut(s) 61
BlsI GCNGC 1 cut(s) 222
BmiI GGNNCC 1 cut(s) 204
BmsI GCATC 2 cut(s) 47, 144
BpiI GAAGAC 1 cut(s) 101
Bpu10I CCTNAGC 1 cut(s) 210
Bpu1102I GCTNAGC 1 cut(s) 61
BsaJI CCNNGG 1 cut(s) 116
Bse118I RCCGGY 1 cut(s) 65
BseDI CCNNGG 1 cut(s) 116
BseGI GGATG 2 cut(s) 10, 62
BseMII CTCAG 1 cut(s) 75
BseXI GCAGC 1 cut(s) 232
BsiSI CCGG 1 cut(s) 66
Bsp1720I GCTNAGC 1 cut(s) 61
BspACI CCGC 1 cut(s) 69
BspCNI CTCAG 1 cut(s) 74
BspLI GGNNCC 1 cut(s) 204
BsrFI RCCGGY 1 cut(s) 65
BssAI RCCGGY 1 cut(s) 65
BssECI CCNNGG 1 cut(s) 116
BssT1I CCWWGG 1 cut(s) 116
Bst4CI ACNGT 2 cut(s) 92, 138
BstC8I GCNNGC 1 cut(s) 67
BstDEI CTNAG 2 cut(s) 61, 210
BstF5I GGATG 2 cut(s) 10, 62
BstH2I RGCGCY 1 cut(s) 47
BstHHI GCGC 1 cut(s) 46
BstMWI GCNNNNNNNGC 2 cut(s) 66, 229
BstV1I GCAGC 1 cut(s) 232
BstV2I GAAGAC 1 cut(s) 101
BtsCI GGATG 2 cut(s) 10, 62
BtsIMutI CAGTG 1 cut(s) 88
Cac8I GCNNGC 1 cut(s) 67
CfoI GCGC 1 cut(s) 46
Cfr10I RCCGGY 1 cut(s) 65
CviAII CATG 1 cut(s) 148
CviJI RGCY 5 cut(s) 24, 65, 209, 223, 232
CviKI_1 RGCY 5 cut(s) 24, 65, 209, 223, 232
DdeI CTNAG 2 cut(s) 61, 210
Eco130I CCWWGG 1 cut(s) 116
EcoT14I CCWWGG 1 cut(s) 116
ErhI CCWWGG 1 cut(s) 116
FaeI CATG 1 cut(s) 151
FaiI YATR 2 cut(s) 86, 149
FatI CATG 1 cut(s) 147
FauI CCCGC 1 cut(s) 62
Fnu4HI GCNGC 1 cut(s) 221
FokI GGATG 2 cut(s) 17, 69
Fsp4HI GCNGC 1 cut(s) 221
FspBI CTAG 3 cut(s) 117, 266, 279
GlaI GCGC 1 cut(s) 45
GluI GCNGC 1 cut(s) 221
HaeII RGCGCY 1 cut(s) 47
HapII CCGG 1 cut(s) 66
HhaI GCGC 1 cut(s) 46
Hin1II CATG 1 cut(s) 151
Hin6I GCGC 1 cut(s) 44
HinP1I GCGC 1 cut(s) 44
HindIII AAGCTT 1 cut(s) 230
HinfI GANTC 3 cut(s) 122, 130, 185
HpaII CCGG 1 cut(s) 66
Hpy188III TCNNGA 1 cut(s) 54
HpyCH4III ACNGT 2 cut(s) 92, 138
HpyCH4V TGCA 3 cut(s) 157, 178, 194
HpyF10VI GCNNNNNNNGC 2 cut(s) 66, 229
HpyF3I CTNAG 2 cut(s) 61, 210
Hsp92II CATG 1 cut(s) 151
HspAI GCGC 1 cut(s) 44
KroI GCCGGC 1 cut(s) 65
KroNI GCCGGC 1 cut(s) 67
LpnPI CCDG 6 cut(s) 26, 39, 79, 143, 219, 234
Lsp1109I GCAGC 1 cut(s) 232
LweI GCATC 2 cut(s) 47, 144
MaeI CTAG 3 cut(s) 117, 266, 279
MboII GAAGA 1 cut(s) 101
MfeI CAATTG 1 cut(s) 18
MluCI AATT 1 cut(s) 18
MnlI CCTC 2 cut(s) 98, 306
MroNI GCCGGC 1 cut(s) 65
MslI CAYNNNNRTG 2 cut(s) 93, 152
MspI CCGG 1 cut(s) 66
MunI CAATTG 1 cut(s) 18
MwoI GCNNNNNNNGC 2 cut(s) 66, 229
NaeI GCCGGC 1 cut(s) 67
NgoMIV GCCGGC 1 cut(s) 65
NlaIII CATG 1 cut(s) 151
NlaIV GGNNCC 1 cut(s) 204
PdiI GCCGGC 1 cut(s) 67
PfeI GAWTC 3 cut(s) 122, 130, 185
PkrI GCNGC 1 cut(s) 222
PspN4I GGNNCC 1 cut(s) 204
RseI CAYNNNNRTG 2 cut(s) 93, 152
SatI GCNGC 1 cut(s) 221
SetI ASST 5 cut(s) 109, 118, 211, 225, 234
SfaNI GCATC 2 cut(s) 47, 144
SmiMI CAYNNNNRTG 2 cut(s) 93, 152
Sse9I AATT 1 cut(s) 18
SsiI CCGC 1 cut(s) 69
SspMI CTAG 3 cut(s) 117, 266, 279
StyI CCWWGG 1 cut(s) 116
TaaI ACNGT 2 cut(s) 92, 138
TaqI TCGA 1 cut(s) 103
TasI AATT 1 cut(s) 18
TfiI GAWTC 3 cut(s) 122, 130, 185
TscAI CASTG 1 cut(s) 95
TseI GCWGC 1 cut(s) 220
TspDTI ATGAA 2 cut(s) 21, 246
TspRI CASTG 1 cut(s) 95
XmaJI CCTAGG 1 cut(s) 116
XspI CTAG 3 cut(s) 117, 266, 279
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.