Rorug03G0083500

Hydrolyzes acetyl esters in homogalacturonan regions of pectin. In type I primary cell wall, galacturonic acid residues of pectin can be acetylated at the O-2 and O-3 positions. Decreasing the degree of acetylation of pectin gels in vitro alters their physical properties

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000003
OG0001587
Physical Location & Seq
Reverse (-)
6482604 .. 6483338
735 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug03G0083500.1

Sequence Viewer

Length: 735 bp
ATGATTCTTCCTTTTCATAAAAACAACCACAACCCAATCAGAATTTTGATTCCCACCCAAGATTGGCCTTCACTTTTCACCCACAGGGCATCTTTGTTTTTTCTCCTATTAATCTTGGCTCTCTCCTCCTCCTTCCCCATTGAAGCCATGAAGGTCCATCCAATCCCCAAGAGGCGCAACATCTCCATCCAGTACCATTCAAGAAGCCCACTCTCTGAAGCCCAGGCCCTCCTCGGTCTCACCCACAAGAAGCTCCGCCGCCTCCCCCATGTCTTCAGCCGCGTCCTCGAGCTCCCTTTCCGCTCCGACGCCGACGTCCTCATCGAGGAGAACCCCGACTGCTTCCACTTCGTCGTCAAAACCGAGAGCTTCGGCCACGACGTCAGGGCCCACACCGTCGAAATCCACCCCGGGGTGACCAAGATTGTCGTCAGGGAAAGAGGGTCCGCGGCGGAGCTGACGTTGGACGAGCTGGAGTTGGACATGTGGAGGTTCCGCCTGCCGGAGTCGACCCGGACGGAGCTGGCCAGGGCGGTTTTTGTCGACGGGGAGCTCATTGTGACGGTGCAGAAGATGGAGGGGATGGAGAATTCGGAGGGCGGAGATGGTGGGGAGGTTTGGGGCGGTGGTGGTGGGAATGGGAATGAGGGTTCAGAGGAGGTATGGGAAATCGGCTTGTGCTTGTACATTTCAGCTGCAAATCTGGGTTTTCAGATTTTGATATTTTGGGGCTGA

Protein Analysis

244

Amino Acids

27.48

Weight (kDa)

5.88

Isoelectric Point (pI)

51.72

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 314
AatII GACGTC 2 cut(s) 318, 384
AccBSI CCGCTC 1 cut(s) 303
AccI GTMKAC 2 cut(s) 509, 543
AccII CGCG 2 cut(s) 282, 449
AcoI YGGCCR 2 cut(s) 373, 525
AcsI RAATTY 2 cut(s) 42, 589
AcuI CTGAAG 2 cut(s) 237, 259
AcyI GRCGYC 3 cut(s) 309, 315, 381
AfaI GTAC 2 cut(s) 194, 686
AfiI CCNNNNNNNGG 4 cut(s) 63, 325, 412, 502
AflIII ACRYGT 1 cut(s) 483
AgsI TTSAA 2 cut(s) 143, 201
AjnI CCWGG 2 cut(s) 222, 527
AloI GAACNNNNNNTCC 2 cut(s) 634, 666
AluBI AGCT 8 cut(s) 253, 292, 369, 457, 472, 523, 553, 695
AluI AGCT 8 cut(s) 253, 292, 369, 457, 472, 523, 553, 695
Alw21I GWGCWC 2 cut(s) 294, 555
Alw26I GTCTC 1 cut(s) 242
Ama87I CYCGRG 2 cut(s) 287, 410
AoxI GGCC 5 cut(s) 65, 225, 373, 387, 525
ApaI GGGCCC 1 cut(s) 391
ApeKI GCWGC 1 cut(s) 695
ApoI RAATTY 2 cut(s) 42, 589
AseI ATTAAT 1 cut(s) 110
AspLEI GCGC 1 cut(s) 177
AspS9I GGNCC 5 cut(s) 154, 226, 387, 388, 444
AsuC2I CCSGG 3 cut(s) 411, 412, 514
AsuHPI GGTGA 3 cut(s) 70, 232, 427
AvaI CYCGRG 2 cut(s) 287, 410
AvaII GGWCC 2 cut(s) 154, 444
BaeGI GKGCMC 1 cut(s) 391
BalI TGGCCA 1 cut(s) 527
BanII GRGCYC 3 cut(s) 294, 391, 555
BbsI GAAGAC 1 cut(s) 265
Bbv12I GWGCWC 2 cut(s) 294, 555
BbvI GCAGC 1 cut(s) 682
BccI CCATC 5 cut(s) 165, 194, 568, 577, 599
BciT130I CCWGG 2 cut(s) 224, 529
BcnI CCSGG 3 cut(s) 411, 412, 514
BcoDI GTCTC 1 cut(s) 242
BisI GCNGC 4 cut(s) 259, 280, 450, 696
BlsI GCNGC 4 cut(s) 260, 281, 451, 697
Bme1390I CCNGG 5 cut(s) 224, 411, 412, 514, 529
Bme18I GGWCC 2 cut(s) 154, 444
BmeT110I CYCGRG 2 cut(s) 287, 410
BmgT120I GGNCC 5 cut(s) 154, 226, 387, 388, 444
BmiI GGNNCC 3 cut(s) 389, 445, 494
BmrFI CCNGG 5 cut(s) 224, 411, 412, 514, 529
BmsI GCATC 1 cut(s) 98
BpiI GAAGAC 1 cut(s) 265
BpmI CTGGAG 1 cut(s) 494
BpuMI CCSGG 3 cut(s) 411, 412, 514
BsaHI GRCGYC 3 cut(s) 309, 315, 381
BsaI GGTCTC 1 cut(s) 242
BsaJI CCNNGG 7 cut(s) 222, 232, 409, 410, 411, 447, 528
BsaXI ACNNNNNCTCC 2 cut(s) 446, 476
Bsc4I CCNNNNNNNGG 4 cut(s) 63, 325, 412, 502
Bse1I ACTGG 1 cut(s) 190
BseBI CCWGG 2 cut(s) 224, 529
BseDI CCNNGG 7 cut(s) 222, 232, 409, 410, 411, 447, 528
BseGI GGATG 3 cut(s) 157, 186, 588
BseLI CCNNNNNNNGG 4 cut(s) 63, 325, 412, 502
BseNI ACTGG 1 cut(s) 190
BseRI GAGGAG 5 cut(s) 115, 118, 221, 341, 671
BseSI GKGCMC 1 cut(s) 391
BseXI GCAGC 1 cut(s) 682
BsgI GTGCAG 1 cut(s) 587
Bsh1236I CGCG 2 cut(s) 282, 449
BshFI GGCC 5 cut(s) 67, 227, 375, 389, 527
BsiHKAI GWGCWC 2 cut(s) 294, 555
BsiHKCI CYCGRG 2 cut(s) 287, 410
BsiSI CCGG 3 cut(s) 411, 503, 514
BslI CCNNNNNNNGG 4 cut(s) 63, 325, 412, 502
BsmAI GTCTC 1 cut(s) 242
BsnI GGCC 5 cut(s) 67, 227, 375, 389, 527
Bso31I GGTCTC 1 cut(s) 242
BsoBI CYCGRG 2 cut(s) 287, 410
Bsp120I GGGCCC 1 cut(s) 387
Bsp1286I GDGCHC 3 cut(s) 294, 391, 555
Bsp1407I TGTACA 1 cut(s) 684
BspANI GGCC 5 cut(s) 67, 227, 375, 389, 527
BspFNI CGCG 2 cut(s) 282, 449
BspLI GGNNCC 3 cut(s) 389, 445, 494
BspTNI GGTCTC 1 cut(s) 242
BsrBI CCGCTC 1 cut(s) 303
BsrGI TGTACA 1 cut(s) 684
BsrI ACTGG 1 cut(s) 190
BssECI CCNNGG 7 cut(s) 222, 232, 409, 410, 411, 447, 528
BssNI GRCGYC 3 cut(s) 309, 315, 381
Bst2UI CCWGG 2 cut(s) 224, 529
Bst4CI ACNGT 2 cut(s) 397, 565
BstACI GRCGYC 3 cut(s) 309, 315, 381
BstAUI TGTACA 1 cut(s) 684
BstC8I GCNNGC 2 cut(s) 500, 525
BstDSI CCRYGG 1 cut(s) 447
BstEII GGTNACC 1 cut(s) 415
BstENI CCTNNNNNAGG 1 cut(s) 323
BstF5I GGATG 3 cut(s) 157, 186, 588
BstFNI CGCG 2 cut(s) 282, 449
BstHHI GCGC 1 cut(s) 177
BstMAI GTCTC 1 cut(s) 242
BstNI CCWGG 2 cut(s) 224, 529
BstNSI RCATGY 1 cut(s) 487
BstPI GGTNACC 1 cut(s) 415
BstSCI CCNGG 5 cut(s) 222, 409, 410, 512, 527
BstSLI GKGCMC 1 cut(s) 391
BstUI CGCG 2 cut(s) 282, 449
BstV1I GCAGC 1 cut(s) 682
BstV2I GAAGAC 1 cut(s) 265
BsuRI GGCC 5 cut(s) 67, 227, 375, 389, 527
BtgI CCRYGG 1 cut(s) 447
BtsCI GGATG 3 cut(s) 157, 186, 588
Cac8I GCNNGC 2 cut(s) 500, 525
CfoI GCGC 1 cut(s) 177
Cfr13I GGNCC 5 cut(s) 154, 226, 387, 388, 444
Cfr42I CCGCGG 1 cut(s) 450
Cfr9I CCCGGG 1 cut(s) 410
CseI GACGC 2 cut(s) 271, 317
Csp6I GTAC 2 cut(s) 193, 685
CviAII CATG 3 cut(s) 148, 269, 484
CviQI GTAC 2 cut(s) 193, 685
DrdI GACNNNNNNGTC 1 cut(s) 314
DseDI GACNNNNNNGTC 1 cut(s) 314
EaeI YGGCCR 2 cut(s) 373, 525
EciI GGCGGA 4 cut(s) 245, 467, 485, 615
Ecl136II GAGCTC 2 cut(s) 292, 553
Eco24I GRGCYC 3 cut(s) 294, 391, 555
Eco31I GGTCTC 1 cut(s) 242
Eco47I GGWCC 2 cut(s) 154, 444
Eco53kI GAGCTC 2 cut(s) 292, 553
Eco57I CTGAAG 2 cut(s) 237, 259
Eco88I CYCGRG 2 cut(s) 287, 410
Eco91I GGTNACC 1 cut(s) 415
EcoICRI GAGCTC 2 cut(s) 292, 553
EcoNI CCTNNNNNAGG 1 cut(s) 323
EcoO109I RGGNCCY 2 cut(s) 226, 387
EcoO65I GGTNACC 1 cut(s) 415
EcoRI GAATTC 1 cut(s) 589
EcoRII CCWGG 2 cut(s) 222, 527
EcoT38I GRGCYC 3 cut(s) 294, 391, 555
FaeI CATG 3 cut(s) 151, 272, 487
FaiI YATR 5 cut(s) 18, 149, 270, 485, 664
FatI CATG 3 cut(s) 147, 268, 483
FblI GTMKAC 2 cut(s) 509, 543
Fnu4HI GCNGC 4 cut(s) 259, 280, 450, 696
FokI GGATG 3 cut(s) 144, 173, 595
FriOI GRGCYC 3 cut(s) 294, 391, 555
Fsp4HI GCNGC 4 cut(s) 259, 280, 450, 696
GlaI GCGC 1 cut(s) 176
GluI GCNGC 4 cut(s) 259, 280, 450, 696
GsuI CTGGAG 1 cut(s) 494
HaeIII GGCC 5 cut(s) 67, 227, 375, 389, 527
HapII CCGG 3 cut(s) 411, 503, 514
HgaI GACGC 2 cut(s) 271, 317
HhaI GCGC 1 cut(s) 177
Hin1I GRCGYC 3 cut(s) 309, 315, 381
Hin1II CATG 3 cut(s) 151, 272, 487
Hin6I GCGC 1 cut(s) 175
HinP1I GCGC 1 cut(s) 175
HincII GTYRAC 2 cut(s) 510, 544
HindII GTYRAC 2 cut(s) 510, 544
HinfI GANTC 3 cut(s) 4, 49, 506
HpaII CCGG 3 cut(s) 411, 503, 514
HphI GGTGA 3 cut(s) 70, 232, 427
Hpy166II GTNNAC 2 cut(s) 510, 544
Hpy188I TCNGA 6 cut(s) 41, 217, 307, 595, 655, 714
Hpy188III TCNNGA 1 cut(s) 201
Hpy8I GTNNAC 2 cut(s) 510, 544
Hpy99I CGWCG 6 cut(s) 311, 317, 356, 383, 401, 548
HpyAV CCTTC 3 cut(s) 78, 142, 145
HpyCH4III ACNGT 2 cut(s) 397, 565
HpyCH4IV ACGT 3 cut(s) 315, 381, 461
HpyCH4V TGCA 2 cut(s) 568, 698
HpySE526I ACGT 3 cut(s) 315, 381, 461
Hsp92I GRCGYC 3 cut(s) 309, 315, 381
Hsp92II CATG 3 cut(s) 151, 272, 487
HspAI GCGC 1 cut(s) 175
KspI CCGCGG 1 cut(s) 450
LmnI GCTCC 6 cut(s) 258, 297, 308, 454, 520, 550
Lsp1109I GCAGC 1 cut(s) 682
LweI GCATC 1 cut(s) 98
MaeII ACGT 3 cut(s) 315, 381, 461
MaeIII GTNAC 2 cut(s) 415, 559
MbiI CCGCTC 1 cut(s) 303
MboII GAAGA 2 cut(s) 265, 583
MhlI GDGCHC 3 cut(s) 294, 391, 555
MlsI TGGCCA 1 cut(s) 527
MluCI AATT 2 cut(s) 42, 589
MluNI TGGCCA 1 cut(s) 527
MlyI GAGTC 1 cut(s) 515
MmeI TCCRAC 3 cut(s) 330, 444, 459
Mox20I TGGCCA 1 cut(s) 527
MscI TGGCCA 1 cut(s) 527
MseI TTAA 1 cut(s) 110
Msp20I TGGCCA 1 cut(s) 527
MspA1I CMGCKG 2 cut(s) 449, 695
MspI CCGG 3 cut(s) 411, 503, 514
MspR9I CCNGG 5 cut(s) 224, 411, 412, 514, 529
MvaI CCWGG 2 cut(s) 224, 529
MvnI CGCG 2 cut(s) 282, 449
NciI CCSGG 3 cut(s) 411, 412, 514
NlaIII CATG 3 cut(s) 151, 272, 487
NlaIV GGNNCC 3 cut(s) 389, 445, 494
NmuCI GTSAC 2 cut(s) 415, 559
NspI RCATGY 1 cut(s) 487
PaeR7I CTCGAG 1 cut(s) 287
PciI ACATGT 1 cut(s) 483
PcsI WCGNNNNNNNCGW 3 cut(s) 321, 360, 378
PfeI GAWTC 2 cut(s) 4, 49
PkrI GCNGC 4 cut(s) 260, 281, 451, 697
PleI GAGTC 1 cut(s) 514
PpsI GAGTC 1 cut(s) 514
PscI ACATGT 1 cut(s) 483
PshBI ATTAAT 1 cut(s) 110
Psp124BI GAGCTC 2 cut(s) 294, 555
Psp6I CCWGG 2 cut(s) 222, 527
PspEI GGTNACC 1 cut(s) 415
PspGI CCWGG 2 cut(s) 222, 527
PspN4I GGNNCC 3 cut(s) 389, 445, 494
PspOMI GGGCCC 1 cut(s) 387
PspPI GGNCC 5 cut(s) 154, 226, 387, 388, 444
PspXI VCTCGAGB 1 cut(s) 287
PvuII CAGCTG 1 cut(s) 695
RsaI GTAC 2 cut(s) 194, 686
RsaNI GTAC 2 cut(s) 193, 685
SacI GAGCTC 2 cut(s) 294, 555
SacII CCGCGG 1 cut(s) 450
SalI GTCGAC 2 cut(s) 508, 542
SaqAI TTAA 1 cut(s) 110
SatI GCNGC 4 cut(s) 259, 280, 450, 696
Sau96I GGNCC 5 cut(s) 154, 226, 387, 388, 444
SchI GAGTC 1 cut(s) 515
ScrFI CCNGG 5 cut(s) 224, 411, 412, 514, 529
SduI GDGCHC 3 cut(s) 294, 391, 555
SfaNI GCATC 1 cut(s) 98
Sfr274I CTCGAG 1 cut(s) 287
Sfr303I CCGCGG 1 cut(s) 450
SgrBI CCGCGG 1 cut(s) 450
SinI GGWCC 2 cut(s) 154, 444
SlaI CTCGAG 1 cut(s) 287
SmaI CCCGGG 1 cut(s) 412
SmlI CTYRAG 1 cut(s) 287
SmoI CTYRAG 1 cut(s) 287
Sse9I AATT 2 cut(s) 42, 589
SstI GAGCTC 2 cut(s) 294, 555
StyD4I CCNGG 5 cut(s) 222, 409, 410, 512, 527
TaaI ACNGT 2 cut(s) 397, 565
TaiI ACGT 3 cut(s) 318, 384, 464
TaqI TCGA 5 cut(s) 288, 324, 399, 509, 543
TaqII GACCGA 1 cut(s) 224
TasI AATT 2 cut(s) 42, 589
TatI WGTACW 1 cut(s) 684
TauI GCSGC 3 cut(s) 261, 282, 452
TfiI GAWTC 2 cut(s) 4, 49
Tru1I TTAA 1 cut(s) 110
Tru9I TTAA 1 cut(s) 110
TseFI GTSAC 2 cut(s) 415, 559
TseI GCWGC 1 cut(s) 695
Tsp45I GTSAC 2 cut(s) 415, 559
TspDTI ATGAA 2 cut(s) 5, 164
TspGWI ACGGA 1 cut(s) 533
TspMI CCCGGG 1 cut(s) 410
VpaK11BI GGWCC 2 cut(s) 154, 444
VspI ATTAAT 1 cut(s) 110
XagI CCTNNNNNAGG 1 cut(s) 323
XapI RAATTY 2 cut(s) 42, 589
XceI RCATGY 1 cut(s) 487
XhoI CTCGAG 1 cut(s) 287
XmaI CCCGGG 1 cut(s) 410
XmiI GTMKAC 2 cut(s) 509, 543
ZraI GACGTC 2 cut(s) 316, 382
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.