AT4G16230

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0001879
Physical Location & Seq
Forward (+)
9185298 .. 9187170
1873 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G16230.1

Sequence Viewer

Length: 756 bp
ATGTCGTTGCTTGTATTTCTTTGCCAAATTATAGTATTATCGGTCTTGTTCTTCTCCGAGGTGTGCCTTGCCGGTAAAAAAATTCCAGCAAATTTTGTGTTCGGAGATTCATTGGTGGATGCCGGAAACAACAACTACTTAGCCACATTGTCAAAGGCTAATTATGTTCCTAATGGGATCGATTTTGGATCACCTACTGGTCGATTCACTAACGGAAGAACAATCGTCGATATCGTATATCAAGCATTAGGTTCCGACGAGTTAACTCCACCATACTTAGCACCAACAACAAGCGGATCTCTCATTCTCAATGGCGTTAATTATGCTTCGGGCGGAAGTGGAATTCTCAATTCCACCGGAAAATTATTTGGCGAACGTATAAATGTGGATGCACAACTAGACAACTTTGCAACGACAAGACAAGACATCATTTCTTGGATCGGTGAATCCGAAGCCGCTAAATTATTCCGGTCAGCAATTTTCTCGGTTACGACAGGTTCTAATGATCTAATCAACAATTATTTCACTCCGGTCATATCAACCCTTCAACGGAAAGTAGTAGCTCCAGAAGTCTTTGTTGATACAATGATCTCAAAATTTCGATTACAACTCACCAGATTGTACCAATTGGGTGCGAGGAAAATCGTGGTGATTAATATTGGTCCCATCGGTTGTATACCATTCGAGAGAGAGTCTGATCCAGCCGCTGGAAATAACTGTTTGGCCGAACCTAATGAGGTTGGCACAAATGTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

251

Amino Acids

27.0

Weight (kDa)

5.05

Isoelectric Point (pI)

20.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 32 - 240 2.2e-17 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 707
AccI GTMKAC 1 cut(s) 676
AciI CCGC 4 cut(s) 294, 333, 456, 705
AclWI GGATC 5 cut(s) 185, 196, 304, 446, 692
AcoI YGGCCR 1 cut(s) 723
AcsI RAATTY 4 cut(s) 81, 91, 342, 596
AfaI GTAC 1 cut(s) 623
AfiI CCNNNNNNNGG 2 cut(s) 549, 707
AgsI TTSAA 1 cut(s) 548
AluBI AGCT 1 cut(s) 563
AluI AGCT 1 cut(s) 563
AlwI GGATC 5 cut(s) 185, 196, 304, 446, 692
AlwNI CAGNNNCTG 1 cut(s) 707
AoxI GGCC 1 cut(s) 723
ApoI RAATTY 4 cut(s) 81, 91, 342, 596
AseI ATTAAT 1 cut(s) 654
AspS9I GGNCC 1 cut(s) 662
AsuHPI GGTGA 4 cut(s) 183, 455, 604, 661
AvaII GGWCC 1 cut(s) 662
BccI CCATC 1 cut(s) 674
BcgI CGANNNNNNTGC 2 cut(s) 465, 499
BfaI CTAG 1 cut(s) 398
BisI GCNGC 2 cut(s) 456, 705
BlsI GCNGC 2 cut(s) 457, 706
Bme18I GGWCC 1 cut(s) 662
BmgT120I GGNCC 1 cut(s) 662
BmiI GGNNCC 2 cut(s) 253, 664
BmsI GCATC 2 cut(s) 109, 379
BpmI CTGGAG 1 cut(s) 549
Bsa29I ATCGAT 1 cut(s) 180
BsaJI CCNNGG 1 cut(s) 57
BsaWI WCCGGW 3 cut(s) 356, 468, 529
Bsc4I CCNNNNNNNGG 2 cut(s) 549, 707
Bse118I RCCGGY 1 cut(s) 71
Bse1I ACTGG 1 cut(s) 202
BseCI ATCGAT 1 cut(s) 180
BseDI CCNNGG 1 cut(s) 57
BseGI GGATG 2 cut(s) 124, 394
BseLI CCNNNNNNNGG 2 cut(s) 549, 707
BseNI ACTGG 1 cut(s) 202
BshFI GGCC 1 cut(s) 725
BshVI ATCGAT 1 cut(s) 180
BsiSI CCGG 5 cut(s) 72, 123, 357, 469, 530
BslFI GGGAC 1 cut(s) 648
BslI CCNNNNNNNGG 2 cut(s) 549, 707
BsmFI GGGAC 1 cut(s) 648
BsnI GGCC 1 cut(s) 725
Bsp143I GATC 7 cut(s) 177, 188, 296, 438, 505, 588, 697
BspACI CCGC 4 cut(s) 294, 333, 456, 705
BspANI GGCC 1 cut(s) 725
BspDI ATCGAT 1 cut(s) 180
BspLI GGNNCC 2 cut(s) 253, 664
BspPI GGATC 5 cut(s) 185, 196, 304, 446, 692
BsrFI RCCGGY 1 cut(s) 71
BsrI ACTGG 1 cut(s) 202
BssAI RCCGGY 1 cut(s) 71
BssECI CCNNGG 1 cut(s) 57
BssMI GATC 7 cut(s) 177, 188, 296, 438, 505, 588, 697
BssNAI GTATAC 1 cut(s) 677
Bst1107I GTATAC 1 cut(s) 677
Bst4CI ACNGT 1 cut(s) 719
BstDEI CTNAG 2 cut(s) 139, 277
BstF5I GGATG 2 cut(s) 124, 394
BstKTI GATC 7 cut(s) 180, 191, 299, 441, 508, 591, 700
BstMBI GATC 7 cut(s) 177, 188, 296, 438, 505, 588, 697
BstX2I RGATCY 1 cut(s) 296
BstYI RGATCY 1 cut(s) 296
BstZ17I GTATAC 1 cut(s) 677
Bsu15I ATCGAT 1 cut(s) 180
BsuRI GGCC 1 cut(s) 725
BsuTUI ATCGAT 1 cut(s) 180
BtsCI GGATG 2 cut(s) 124, 394
CaiI CAGNNNCTG 1 cut(s) 707
Cfr10I RCCGGY 1 cut(s) 71
Cfr13I GGNCC 1 cut(s) 662
ClaI ATCGAT 1 cut(s) 180
Csp6I GTAC 1 cut(s) 622
CviJI RGCY 6 cut(s) 143, 158, 455, 563, 704, 725
CviKI_1 RGCY 6 cut(s) 143, 158, 455, 563, 704, 725
CviQI GTAC 1 cut(s) 622
DdeI CTNAG 2 cut(s) 139, 277
DpnI GATC 7 cut(s) 179, 190, 298, 440, 507, 590, 699
DpnII GATC 7 cut(s) 177, 188, 296, 438, 505, 588, 697
EaeI YGGCCR 1 cut(s) 723
EciI GGCGGA 1 cut(s) 348
Eco32I GATATC 1 cut(s) 232
Eco47I GGWCC 1 cut(s) 662
EcoRI GAATTC 1 cut(s) 342
EcoRV GATATC 1 cut(s) 232
FaiI YATR 9 cut(s) 32, 165, 238, 274, 324, 380, 536, 677, 754
FaqI GGGAC 1 cut(s) 648
FblI GTMKAC 1 cut(s) 676
Fnu4HI GCNGC 2 cut(s) 456, 705
FokI GGATG 2 cut(s) 131, 401
Fsp4HI GCNGC 2 cut(s) 456, 705
FspBI CTAG 1 cut(s) 398
GluI GCNGC 2 cut(s) 456, 705
GsuI CTGGAG 1 cut(s) 549
HaeIII GGCC 1 cut(s) 725
HapII CCGG 5 cut(s) 72, 123, 357, 469, 530
HincII GTYRAC 1 cut(s) 264
HindII GTYRAC 1 cut(s) 264
HinfI GANTC 4 cut(s) 107, 204, 446, 692
HpaI GTTAAC 1 cut(s) 264
HpaII CCGG 5 cut(s) 72, 123, 357, 469, 530
HphI GGTGA 4 cut(s) 183, 455, 604, 661
Hpy166II GTNNAC 2 cut(s) 264, 677
Hpy188I TCNGA 5 cut(s) 58, 104, 256, 451, 697
Hpy188III TCNNGA 2 cut(s) 566, 685
Hpy8I GTNNAC 2 cut(s) 264, 677
Hpy99I CGWCG 2 cut(s) 230, 260
HpyAV CCTTC 1 cut(s) 554
HpyCH4III ACNGT 1 cut(s) 719
HpyCH4IV ACGT 1 cut(s) 376
HpyCH4V TGCA 2 cut(s) 392, 410
HpyF3I CTNAG 2 cut(s) 139, 277
HpySE526I ACGT 1 cut(s) 376
KspAI GTTAAC 1 cut(s) 264
Kzo9I GATC 7 cut(s) 177, 188, 296, 438, 505, 588, 697
LmnI GCTCC 1 cut(s) 568
LweI GCATC 2 cut(s) 109, 379
MaeI CTAG 1 cut(s) 398
MaeII ACGT 1 cut(s) 376
MaeIII GTNAC 1 cut(s) 487
MalI GATC 7 cut(s) 179, 190, 298, 440, 507, 590, 699
MboI GATC 7 cut(s) 177, 188, 296, 438, 505, 588, 697
MboII GAAGA 2 cut(s) 43, 228
MfeI CAATTG 1 cut(s) 626
MflI RGATCY 1 cut(s) 296
MlyI GAGTC 1 cut(s) 701
MmeI TCCRAC 1 cut(s) 279
MnlI CCTC 3 cut(s) 52, 630, 730
MseI TTAA 3 cut(s) 263, 318, 654
MspA1I CMGCKG 1 cut(s) 707
MspI CCGG 5 cut(s) 72, 123, 357, 469, 530
MunI CAATTG 1 cut(s) 626
NdeII GATC 7 cut(s) 177, 188, 296, 438, 505, 588, 697
NlaIV GGNNCC 2 cut(s) 253, 664
PcsI WCGNNNNNNNCGW 2 cut(s) 231, 447
PfeI GAWTC 3 cut(s) 107, 204, 446
PflMI CCANNNNNTGG 1 cut(s) 707
PkrI GCNGC 2 cut(s) 457, 706
PleI GAGTC 1 cut(s) 700
PpsI GAGTC 1 cut(s) 700
PshBI ATTAAT 1 cut(s) 654
PspN4I GGNNCC 2 cut(s) 253, 664
PspPI GGNCC 1 cut(s) 662
PstNI CAGNNNCTG 1 cut(s) 707
PsuI RGATCY 1 cut(s) 296
RsaI GTAC 1 cut(s) 623
RsaNI GTAC 1 cut(s) 622
SaqAI TTAA 3 cut(s) 263, 318, 654
SatI GCNGC 2 cut(s) 456, 705
Sau3AI GATC 7 cut(s) 177, 188, 296, 438, 505, 588, 697
Sau96I GGNCC 1 cut(s) 662
SchI GAGTC 1 cut(s) 701
SetI ASST 8 cut(s) 63, 196, 253, 379, 499, 565, 733, 741
SfaNI GCATC 2 cut(s) 109, 379
SinI GGWCC 1 cut(s) 662
SsiI CCGC 4 cut(s) 294, 333, 456, 705
SspI AATATT 1 cut(s) 658
SspMI CTAG 1 cut(s) 398
TaaI ACNGT 1 cut(s) 719
TaiI ACGT 1 cut(s) 379
TaqI TCGA 5 cut(s) 180, 202, 228, 601, 684
TaqII GACCGA 1 cut(s) 31
TauI GCSGC 2 cut(s) 458, 707
TfiI GAWTC 3 cut(s) 107, 204, 446
Tru1I TTAA 3 cut(s) 263, 318, 654
Tru9I TTAA 3 cut(s) 263, 318, 654
TspDTI ATGAA 1 cut(s) 99
TspGWI ACGGA 2 cut(s) 228, 565
Van91I CCANNNNNTGG 1 cut(s) 707
VpaK11BI GGWCC 1 cut(s) 662
VspI ATTAAT 1 cut(s) 654
XapI RAATTY 4 cut(s) 81, 91, 342, 596
XmiI GTMKAC 1 cut(s) 676
XspI CTAG 1 cut(s) 398
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.