FvH4_4g19851

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0001879
Physical Location & Seq
Forward (+)
23357045 .. 23358773
1729 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g19851.t1

Sequence Viewer

Length: 828 bp
ATGTATACTATACTTTATGGCCGGGCTGTGGCTCCTTTCAAGCTTCATCTGCAGAATAGTATTGATTTAGAGAAATCTGGGCGCGACCCTTTTACGTACAAGACTCTGGGTTTTAAGGGGTCCACTCCTCCTTACTTAGCTCCCACAACATCTGGGCCAGTAATTTTGACGGGTGTCAACTATGCTTCAGGTGGAGGTGGAATTCTTAATGAAACAGGAAAAGTTGGTAGAATAAACTTGGATGCACAGATAGATAACTTCTCAAATACCAGGGAAGACATTATGTCAACCATTGGTGTTCCTGCAGCTCTAAAGCTACTTGAAGGAGCTCTCTTCTCAGTAACAATTGGCTCGAATGATTTCCTCAATAACTACTTGGCGCCAGTAATTTCCGAGGCTGAGAGGAAGTTGATACCTCCGGAAACCTTTGTTGGCATCTTGATTGCAAGACTCGGACTACAAATTACAAGAGTCTACAATTTGGGTGCCAGGAAGATTGTTGTGGCTAATGTAGGACCAATTGGGTGTGTACCCTATCAAAGGGACATAAATAATGTTCCTGCTCCAGATAACAGTGCTGAATTTTCGAATCAATTAGCCCGGTCATACAATGCCAAGCTGAAAATCCTTGTCGCCGCTCTAAACGATCTTCCCGGAGCCAAATTTGTGTATGCAGATGTTTATAGTATTGTAGAAGACATCATTGAGAACTACATATCATATGGTTTTGAGAATGCAAACTCGGCATGCTGCAGAGTAGCAGGGCGTTACGGGGGTCTAATTCCATGCGGTCCTCAGTTCTCAATGGTTTGCGAGGAGCGGTCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

276

Amino Acids

29.65

Weight (kDa)

6.6

Isoelectric Point (pI)

30.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 53 - 255 5.8e-16 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 379, 485
AccBSI CCGCTC 2 cut(s) 638, 820
AccI GTMKAC 2 cut(s) 5, 474
AccII CGCG 1 cut(s) 84
AccIII TCCGGA 1 cut(s) 418
AciI CCGC 3 cut(s) 636, 789, 820
AcoI YGGCCR 1 cut(s) 19
AcsI RAATTY 3 cut(s) 201, 581, 662
AcuI CTGAAG 1 cut(s) 171
AcyI GRCGYC 1 cut(s) 380
AfaI GTAC 2 cut(s) 98, 531
AfiI CCNNNNNNNGG 2 cut(s) 28, 540
AgsI TTSAA 2 cut(s) 40, 323
AhdI GACNNNNNGTC 1 cut(s) 283
AjnI CCWGG 2 cut(s) 269, 488
AjuI GAANNNNNNNTTGG 2 cut(s) 414, 446
AluBI AGCT 6 cut(s) 43, 140, 308, 316, 329, 619
AluI AGCT 6 cut(s) 43, 140, 308, 316, 329, 619
Alw21I GWGCWC 1 cut(s) 331
Aor13HI TCCGGA 1 cut(s) 418
AoxI GGCC 2 cut(s) 19, 155
ApeKI GCWGC 2 cut(s) 305, 750
ApoI RAATTY 3 cut(s) 201, 581, 662
Asp700I GAANNNNTTC 1 cut(s) 359
AspLEI GCGC 2 cut(s) 84, 382
AspS9I GGNCC 5 cut(s) 120, 155, 515, 791, 822
AsuC2I CCSGG 3 cut(s) 23, 601, 654
AsuII TTCGAA 1 cut(s) 587
AvaII GGWCC 4 cut(s) 120, 515, 791, 822
BanI GGYRCC 2 cut(s) 379, 485
BanII GRGCYC 1 cut(s) 331
BbsI GAAGAC 2 cut(s) 282, 702
Bbv12I GWGCWC 1 cut(s) 331
BbvI GCAGC 2 cut(s) 317, 737
BciT130I CCWGG 2 cut(s) 271, 490
BcnI CCSGG 3 cut(s) 23, 601, 654
BfmI CTRYAG 3 cut(s) 50, 303, 751
BfoI RGCGCY 1 cut(s) 383
BisI GCNGC 3 cut(s) 306, 636, 751
BlsI GCNGC 3 cut(s) 307, 637, 752
Bme1390I CCNGG 5 cut(s) 23, 271, 490, 601, 654
Bme18I GGWCC 4 cut(s) 120, 515, 791, 822
BmeRI GACNNNNNGTC 1 cut(s) 283
BmgT120I GGNCC 5 cut(s) 120, 155, 515, 791, 822
BmiI GGNNCC 5 cut(s) 33, 121, 381, 487, 658
BmrFI CCNGG 5 cut(s) 23, 271, 490, 601, 654
BmsI GCATC 2 cut(s) 232, 444
BoxI GACNNNNGTC 1 cut(s) 173
BpiI GAAGAC 2 cut(s) 282, 702
BpmI CTGGAG 1 cut(s) 549
Bpu14I TTCGAA 1 cut(s) 587
BpuMI CCSGG 3 cut(s) 23, 601, 654
BsaAI YACGTR 1 cut(s) 96
BsaHI GRCGYC 1 cut(s) 380
BsaJI CCNNGG 2 cut(s) 270, 393
BsaWI WCCGGW 1 cut(s) 418
Bsc4I CCNNNNNNNGG 2 cut(s) 28, 540
Bse1I ACTGG 2 cut(s) 158, 383
BseAI TCCGGA 1 cut(s) 418
BseBI CCWGG 2 cut(s) 271, 490
BseDI CCNNGG 2 cut(s) 270, 393
BseGI GGATG 1 cut(s) 247
BseLI CCNNNNNNNGG 2 cut(s) 28, 540
BseMII CTCAG 3 cut(s) 351, 390, 809
BseNI ACTGG 2 cut(s) 158, 383
BseRI GAGGAG 1 cut(s) 117
BseXI GCAGC 2 cut(s) 317, 737
Bsh1236I CGCG 1 cut(s) 84
BshFI GGCC 2 cut(s) 21, 157
BshNI GGYRCC 2 cut(s) 379, 485
BsiHKAI GWGCWC 1 cut(s) 331
BsiSI CCGG 4 cut(s) 22, 419, 601, 654
BslFI GGGAC 1 cut(s) 557
BslI CCNNNNNNNGG 2 cut(s) 28, 540
BsmFI GGGAC 1 cut(s) 557
BsmI GAATGC 1 cut(s) 739
BsnI GGCC 2 cut(s) 21, 157
Bsp119I TTCGAA 1 cut(s) 587
Bsp1286I GDGCHC 1 cut(s) 331
Bsp13I TCCGGA 1 cut(s) 418
Bsp143I GATC 1 cut(s) 646
BspACI CCGC 3 cut(s) 636, 789, 820
BspANI GGCC 2 cut(s) 21, 157
BspCNI CTCAG 3 cut(s) 350, 391, 808
BspEI TCCGGA 1 cut(s) 418
BspFNI CGCG 1 cut(s) 84
BspLI GGNNCC 5 cut(s) 33, 121, 381, 487, 658
BspMAI CTGCAG 3 cut(s) 54, 307, 755
BspT104I TTCGAA 1 cut(s) 587
BspT107I GGYRCC 2 cut(s) 379, 485
BsrBI CCGCTC 2 cut(s) 638, 820
BsrI ACTGG 2 cut(s) 158, 383
BssECI CCNNGG 2 cut(s) 270, 393
BssMI GATC 1 cut(s) 646
BssNAI GTATAC 1 cut(s) 6
BssNI GRCGYC 1 cut(s) 380
Bst1107I GTATAC 1 cut(s) 6
Bst2UI CCWGG 2 cut(s) 271, 490
Bst4CI ACNGT 1 cut(s) 575
Bst6I CTCTTC 1 cut(s) 338
BstACI GRCGYC 1 cut(s) 380
BstBAI YACGTR 1 cut(s) 96
BstBI TTCGAA 1 cut(s) 587
BstC8I GCNNGC 1 cut(s) 748
BstDEI CTNAG 4 cut(s) 136, 337, 399, 795
BstENI CCTNNNNNAGG 1 cut(s) 538
BstF5I GGATG 1 cut(s) 247
BstFNI CGCG 1 cut(s) 84
BstH2I RGCGCY 1 cut(s) 383
BstHHI GCGC 2 cut(s) 84, 382
BstKTI GATC 1 cut(s) 649
BstMBI GATC 1 cut(s) 646
BstMWI GCNNNNNNNGC 2 cut(s) 49, 743
BstNI CCWGG 2 cut(s) 271, 490
BstNSI RCATGY 1 cut(s) 750
BstPAI GACNNNNGTC 1 cut(s) 173
BstSCI CCNGG 5 cut(s) 21, 269, 488, 599, 652
BstSFI CTRYAG 3 cut(s) 50, 303, 751
BstSNI TACGTA 1 cut(s) 96
BstUI CGCG 1 cut(s) 84
BstV1I GCAGC 2 cut(s) 317, 737
BstV2I GAAGAC 2 cut(s) 282, 702
BstZ17I GTATAC 1 cut(s) 6
BsuRI GGCC 2 cut(s) 21, 157
BtsCI GGATG 1 cut(s) 247
BtsIMutI CAGTG 1 cut(s) 580
Cac8I GCNNGC 1 cut(s) 748
CfoI GCGC 2 cut(s) 84, 382
Cfr13I GGNCC 5 cut(s) 120, 155, 515, 791, 822
Csp6I GTAC 2 cut(s) 97, 530
CviAII CATG 2 cut(s) 747, 786
CviQI GTAC 2 cut(s) 97, 530
DdeI CTNAG 4 cut(s) 136, 337, 399, 795
DinI GGCGCC 1 cut(s) 381
DpnI GATC 1 cut(s) 648
DpnII GATC 1 cut(s) 646
DriI GACNNNNNGTC 1 cut(s) 283
EaeI YGGCCR 1 cut(s) 19
Eam1104I CTCTTC 1 cut(s) 338
Eam1105I GACNNNNNGTC 1 cut(s) 283
EarI CTCTTC 1 cut(s) 338
Ecl136II GAGCTC 1 cut(s) 329
Eco105I TACGTA 1 cut(s) 96
Eco24I GRGCYC 1 cut(s) 331
Eco47I GGWCC 4 cut(s) 120, 515, 791, 822
Eco53kI GAGCTC 1 cut(s) 329
Eco57I CTGAAG 1 cut(s) 171
EcoICRI GAGCTC 1 cut(s) 329
EcoNI CCTNNNNNAGG 1 cut(s) 538
EcoRI GAATTC 1 cut(s) 201
EcoRII CCWGG 2 cut(s) 269, 488
EcoT38I GRGCYC 1 cut(s) 331
EgeI GGCGCC 1 cut(s) 381
EheI GGCGCC 1 cut(s) 381
FaeI CATG 2 cut(s) 750, 789
FaqI GGGAC 1 cut(s) 557
FatI CATG 2 cut(s) 746, 785
FauNDI CATATG 1 cut(s) 721
FblI GTMKAC 2 cut(s) 5, 474
Fnu4HI GCNGC 3 cut(s) 306, 636, 751
FokI GGATG 1 cut(s) 254
FriOI GRGCYC 1 cut(s) 331
Fsp4HI GCNGC 3 cut(s) 306, 636, 751
GlaI GCGC 2 cut(s) 83, 381
GluI GCNGC 3 cut(s) 306, 636, 751
GsuI CTGGAG 1 cut(s) 549
HaeII RGCGCY 1 cut(s) 383
HaeIII GGCC 2 cut(s) 21, 157
HapII CCGG 4 cut(s) 22, 419, 601, 654
HhaI GCGC 2 cut(s) 84, 382
Hin1I GRCGYC 1 cut(s) 380
Hin1II CATG 2 cut(s) 750, 789
Hin6I GCGC 2 cut(s) 82, 380
HinP1I GCGC 2 cut(s) 82, 380
HincII GTYRAC 2 cut(s) 178, 288
HindII GTYRAC 2 cut(s) 178, 288
HindIII AAGCTT 1 cut(s) 41
HinfI GANTC 4 cut(s) 103, 450, 471, 589
HpaII CCGG 4 cut(s) 22, 419, 601, 654
Hpy166II GTNNAC 6 cut(s) 6, 123, 178, 288, 475, 530
Hpy188I TCNGA 2 cut(s) 394, 455
Hpy188III TCNNGA 3 cut(s) 419, 439, 566
Hpy8I GTNNAC 6 cut(s) 6, 123, 178, 288, 475, 530
HpyAV CCTTC 1 cut(s) 317
HpyCH4III ACNGT 1 cut(s) 575
HpyCH4IV ACGT 1 cut(s) 95
HpyCH4V TGCA 7 cut(s) 52, 245, 305, 446, 674, 737, 753
HpyF10VI GCNNNNNNNGC 2 cut(s) 49, 743
HpyF3I CTNAG 4 cut(s) 136, 337, 399, 795
HpySE526I ACGT 1 cut(s) 95
Hsp92I GRCGYC 1 cut(s) 380
Hsp92II CATG 2 cut(s) 750, 789
HspAI GCGC 2 cut(s) 82, 380
KasI GGCGCC 1 cut(s) 379
Kpn2I TCCGGA 1 cut(s) 418
Kzo9I GATC 1 cut(s) 646
LmnI GCTCC 6 cut(s) 37, 145, 326, 568, 656, 817
Lsp1109I GCAGC 2 cut(s) 317, 737
LweI GCATC 2 cut(s) 232, 444
MaeII ACGT 1 cut(s) 95
MaeIII GTNAC 2 cut(s) 340, 767
MalI GATC 1 cut(s) 648
MbiI CCGCTC 2 cut(s) 638, 820
MboI GATC 1 cut(s) 646
MboII GAAGA 5 cut(s) 287, 325, 505, 641, 707
MfeI CAATTG 2 cut(s) 345, 519
MhlI GDGCHC 1 cut(s) 331
Mly113I GGCGCC 1 cut(s) 380
MlyI GAGTC 3 cut(s) 97, 444, 480
MnlI CCTC 8 cut(s) 138, 188, 374, 388, 396, 426, 804, 808
MroI TCCGGA 1 cut(s) 418
MroXI GAANNNNTTC 1 cut(s) 359
MseI TTAA 2 cut(s) 114, 207
MspI CCGG 4 cut(s) 22, 419, 601, 654
MspR9I CCNGG 5 cut(s) 23, 271, 490, 601, 654
MunI CAATTG 2 cut(s) 345, 519
Mva1269I GAATGC 1 cut(s) 739
MvaI CCWGG 2 cut(s) 271, 490
MvnI CGCG 1 cut(s) 84
MwoI GCNNNNNNNGC 2 cut(s) 49, 743
NarI GGCGCC 1 cut(s) 380
NciI CCSGG 3 cut(s) 23, 601, 654
NdeI CATATG 1 cut(s) 721
NdeII GATC 1 cut(s) 646
NlaIII CATG 2 cut(s) 750, 789
NlaIV GGNNCC 5 cut(s) 33, 121, 381, 487, 658
NmeAIII GCCGAG 1 cut(s) 722
NspI RCATGY 1 cut(s) 750
NspV TTCGAA 1 cut(s) 587
PaeI GCATGC 1 cut(s) 750
PctI GAATGC 1 cut(s) 739
PdmI GAANNNNTTC 1 cut(s) 359
PfeI GAWTC 1 cut(s) 589
PfoI TCCNGGA 1 cut(s) 652
PkrI GCNGC 3 cut(s) 307, 637, 752
PleI GAGTC 3 cut(s) 97, 444, 479
PluTI GGCGCC 1 cut(s) 383
PpsI GAGTC 3 cut(s) 97, 444, 479
Ppu21I YACGTR 1 cut(s) 96
PshAI GACNNNNGTC 1 cut(s) 173
Psp124BI GAGCTC 1 cut(s) 331
Psp6I CCWGG 2 cut(s) 269, 488
PspGI CCWGG 2 cut(s) 269, 488
PspN4I GGNNCC 5 cut(s) 33, 121, 381, 487, 658
PspPI GGNCC 5 cut(s) 120, 155, 515, 791, 822
PstI CTGCAG 3 cut(s) 54, 307, 755
RsaI GTAC 2 cut(s) 98, 531
RsaNI GTAC 2 cut(s) 97, 530
SacI GAGCTC 1 cut(s) 331
SaqAI TTAA 2 cut(s) 114, 207
SatI GCNGC 3 cut(s) 306, 636, 751
Sau3AI GATC 1 cut(s) 646
Sau96I GGNCC 5 cut(s) 120, 155, 515, 791, 822
SchI GAGTC 3 cut(s) 97, 444, 480
ScrFI CCNGG 5 cut(s) 23, 271, 490, 601, 654
SduI GDGCHC 1 cut(s) 331
SfaNI GCATC 2 cut(s) 232, 444
SfcI CTRYAG 3 cut(s) 50, 303, 751
SfoI GGCGCC 1 cut(s) 381
SfuI TTCGAA 1 cut(s) 587
SinI GGWCC 4 cut(s) 120, 515, 791, 822
SnaBI TACGTA 1 cut(s) 96
SphI GCATGC 1 cut(s) 750
SsiI CCGC 3 cut(s) 636, 789, 820
SspDI GGCGCC 1 cut(s) 379
SstI GAGCTC 1 cut(s) 331
StyD4I CCNGG 5 cut(s) 21, 269, 488, 599, 652
TaaI ACNGT 1 cut(s) 575
TaiI ACGT 1 cut(s) 98
TaqI TCGA 2 cut(s) 353, 587
TauI GCSGC 1 cut(s) 638
TfiI GAWTC 1 cut(s) 589
Tru1I TTAA 2 cut(s) 114, 207
Tru9I TTAA 2 cut(s) 114, 207
TscAI CASTG 1 cut(s) 580
TseI GCWGC 2 cut(s) 305, 750
TspDTI ATGAA 2 cut(s) 35, 225
TspRI CASTG 1 cut(s) 580
VpaK11BI GGWCC 4 cut(s) 120, 515, 791, 822
XagI CCTNNNNNAGG 1 cut(s) 538
XapI RAATTY 3 cut(s) 201, 581, 662
XceI RCATGY 1 cut(s) 750
XmiI GTMKAC 2 cut(s) 5, 474
XmnI GAANNNNTTC 1 cut(s) 359
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.