Rh4AG261800

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4A
OG0001879
Physical Location & Seq
Forward (+)
57773306 .. 57774475
1170 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4AG261800.1

Sequence Viewer

Length: 819 bp
ATGTGTTCAGCAAAAGAAACTCCTGCTAATTTTGTGTTTGGCGATTCTCTAGTTGAAGTGGGGAACAACAACTATATTGTGACTCTCTCCAAGGCTGATTATCCTCCCAATGGAATCGATTTTGGAAAGCCTACCGGGAGATACACAAATGGAAGAACCATCATTGACATTATAGGTCAGACTCTTGGTTTTAAGGGGTTCACTCCTCCTTACTTAGCTCCCACAACAGCTGGACCAGTAATTCTGAGTGGTGTTAACTATGCTTCAGGTGGAGGTGGAATTCTTAATGAAACAGGAAAAATATTTGTTGGTAGAATAAACTTGGATGCACAGATAGATAACTTTGCAAATACCAGGCAAGACATTATCTCAAGAATCGATCTTCCTGCAGCTCTAAAGTTACTTAGAAGAGCTCTGTTCTCAGTAACAATCGGCTCGAATGATTTCATCAATAACTACTTGGCGCCAATTATATCTGAGACGGAGAGGAAGTCGATACCTCCGGAAACCTTTGTTGGCATTTTGATCGCAAGACTCAGACTACAAATTACAAGACTGTACAATTTGGGTGCAAGGAAGATGGTTGTGGTAAATGTAGGACCTATTGGGTGTATACCTTATCAGAGGGAAGTAAATCAAGCTGATCCAGAGAGCTGTGTGGAATTTTCGAATCAACTAGCACGGTCATTCAATGCCAAGCTCAGGACCCTTGTCACCGAGCTAAACAAGAACCTTCCTACGGCCAATTTTGTTTATGCAGATGTTTATAGTATTGTGGAAGACATTATTGAGAACTACATATCATATGGTGAGACTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

272

Amino Acids

29.76

Weight (kDa)

6.38

Isoelectric Point (pI)

26.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 11 - 261 5.9e-25 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 463
AccI GTMKAC 1 cut(s) 613
AccIII TCCGGA 1 cut(s) 502
AclWI GGATC 1 cut(s) 638
AcoI YGGCCR 1 cut(s) 741
AcsI RAATTY 2 cut(s) 279, 662
AcuI CTGAAG 1 cut(s) 249
AcyI GRCGYC 1 cut(s) 464
AfaI GTAC 1 cut(s) 560
AfiI CCNNNNNNNGG 3 cut(s) 110, 702, 739
AgsI TTSAA 2 cut(s) 56, 691
AjnI CCWGG 1 cut(s) 353
AjuI GAANNNNNNNTTGG 2 cut(s) 498, 530
AluBI AGCT 8 cut(s) 218, 230, 392, 413, 641, 654, 700, 721
AluI AGCT 8 cut(s) 218, 230, 392, 413, 641, 654, 700, 721
Alw21I GWGCWC 1 cut(s) 415
Alw26I GTCTC 2 cut(s) 473, 806
AlwI GGATC 1 cut(s) 638
Aor13HI TCCGGA 1 cut(s) 502
AoxI GGCC 1 cut(s) 741
ApeKI GCWGC 1 cut(s) 389
ApoI RAATTY 2 cut(s) 279, 662
Asp700I GAANNNNTTC 1 cut(s) 443
AspLEI GCGC 1 cut(s) 466
AspS9I GGNCC 3 cut(s) 233, 599, 705
AsuC2I CCSGG 1 cut(s) 136
AsuHPI GGTGA 1 cut(s) 706
AsuII TTCGAA 1 cut(s) 668
AvaII GGWCC 3 cut(s) 233, 599, 705
BanI GGYRCC 1 cut(s) 463
BanII GRGCYC 1 cut(s) 415
BbsI GAAGAC 1 cut(s) 786
Bbv12I GWGCWC 1 cut(s) 415
BbvI GCAGC 1 cut(s) 401
BccI CCATC 2 cut(s) 167, 574
BceAI ACGGC 1 cut(s) 756
BcgI CGANNNNNNTGC 4 cut(s) 368, 402, 508, 542
BciT130I CCWGG 1 cut(s) 355
BcnI CCSGG 1 cut(s) 136
BcoDI GTCTC 2 cut(s) 473, 806
BfaI CTAG 2 cut(s) 50, 677
BfmI CTRYAG 1 cut(s) 387
BfoI RGCGCY 1 cut(s) 467
BisI GCNGC 1 cut(s) 390
BlsI GCNGC 1 cut(s) 391
Bme1390I CCNGG 2 cut(s) 136, 355
Bme18I GGWCC 3 cut(s) 233, 599, 705
BmgT120I GGNCC 3 cut(s) 233, 599, 705
BmiI GGNNCC 2 cut(s) 465, 707
BmrFI CCNGG 2 cut(s) 136, 355
BmsI GCATC 1 cut(s) 316
BoxI GACNNNNGTC 1 cut(s) 710
BpiI GAAGAC 1 cut(s) 786
Bpu10I CCTNAGC 1 cut(s) 701
Bpu14I TTCGAA 1 cut(s) 668
BpuEI CTTGAG 1 cut(s) 355
BpuMI CCSGG 1 cut(s) 136
Bsa29I ATCGAT 2 cut(s) 117, 378
BsaHI GRCGYC 1 cut(s) 464
BsaJI CCNNGG 1 cut(s) 90
BsaWI WCCGGW 1 cut(s) 502
BsaXI ACNNNNNCTCC 2 cut(s) 476, 506
Bsc4I CCNNNNNNNGG 3 cut(s) 110, 702, 739
Bse1I ACTGG 1 cut(s) 236
BseAI TCCGGA 1 cut(s) 502
BseBI CCWGG 1 cut(s) 355
BseCI ATCGAT 2 cut(s) 117, 378
BseDI CCNNGG 1 cut(s) 90
BseGI GGATG 1 cut(s) 331
BseLI CCNNNNNNNGG 3 cut(s) 110, 702, 739
BseMII CTCAG 5 cut(s) 236, 435, 468, 550, 715
BseNI ACTGG 1 cut(s) 236
BseRI GAGGAG 1 cut(s) 195
BseXI GCAGC 1 cut(s) 401
BshFI GGCC 1 cut(s) 743
BshNI GGYRCC 1 cut(s) 463
BshVI ATCGAT 2 cut(s) 117, 378
BsiHKAI GWGCWC 1 cut(s) 415
BsiSI CCGG 2 cut(s) 135, 503
BslI CCNNNNNNNGG 3 cut(s) 110, 702, 739
BsmAI GTCTC 2 cut(s) 473, 806
BsmBI CGTCTC 1 cut(s) 473
BsnI GGCC 1 cut(s) 743
Bsp119I TTCGAA 1 cut(s) 668
Bsp1286I GDGCHC 1 cut(s) 415
Bsp13I TCCGGA 1 cut(s) 502
Bsp1407I TGTACA 1 cut(s) 558
Bsp143I GATC 3 cut(s) 379, 525, 643
BspANI GGCC 1 cut(s) 743
BspCNI CTCAG 5 cut(s) 237, 434, 469, 549, 714
BspDI ATCGAT 2 cut(s) 117, 378
BspEI TCCGGA 1 cut(s) 502
BspLI GGNNCC 2 cut(s) 465, 707
BspMAI CTGCAG 1 cut(s) 391
BspPI GGATC 1 cut(s) 638
BspQI GCTCTTC 1 cut(s) 403
BspT104I TTCGAA 1 cut(s) 668
BspT107I GGYRCC 1 cut(s) 463
BsrGI TGTACA 1 cut(s) 558
BsrI ACTGG 1 cut(s) 236
BssECI CCNNGG 1 cut(s) 90
BssMI GATC 3 cut(s) 379, 525, 643
BssNAI GTATAC 1 cut(s) 614
BssNI GRCGYC 1 cut(s) 464
BssT1I CCWWGG 1 cut(s) 90
Bst1107I GTATAC 1 cut(s) 614
Bst2UI CCWGG 1 cut(s) 355
Bst4CI ACNGT 2 cut(s) 558, 684
Bst6I CTCTTC 1 cut(s) 403
BstACI GRCGYC 1 cut(s) 464
BstAUI TGTACA 1 cut(s) 558
BstBI TTCGAA 1 cut(s) 668
BstDEI CTNAG 8 cut(s) 214, 245, 404, 421, 477, 536, 701, 816
BstF5I GGATG 1 cut(s) 331
BstH2I RGCGCY 1 cut(s) 467
BstHHI GCGC 1 cut(s) 466
BstKTI GATC 3 cut(s) 382, 528, 646
BstMAI GTCTC 2 cut(s) 473, 806
BstMBI GATC 3 cut(s) 379, 525, 643
BstNI CCWGG 1 cut(s) 355
BstPAI GACNNNNGTC 1 cut(s) 710
BstSCI CCNGG 2 cut(s) 134, 353
BstSFI CTRYAG 1 cut(s) 387
BstV1I GCAGC 1 cut(s) 401
BstV2I GAAGAC 1 cut(s) 786
BstZ17I GTATAC 1 cut(s) 614
Bsu15I ATCGAT 2 cut(s) 117, 378
BsuRI GGCC 1 cut(s) 743
BsuTUI ATCGAT 2 cut(s) 117, 378
BtsCI GGATG 1 cut(s) 331
CfoI GCGC 1 cut(s) 466
Cfr13I GGNCC 3 cut(s) 233, 599, 705
ClaI ATCGAT 2 cut(s) 117, 378
Csp6I GTAC 1 cut(s) 559
CviQI GTAC 1 cut(s) 559
DdeI CTNAG 8 cut(s) 214, 245, 404, 421, 477, 536, 701, 816
DinI GGCGCC 1 cut(s) 465
DpnI GATC 3 cut(s) 381, 527, 645
DpnII GATC 3 cut(s) 379, 525, 643
EaeI YGGCCR 1 cut(s) 741
Eam1104I CTCTTC 1 cut(s) 403
EarI CTCTTC 1 cut(s) 403
Ecl136II GAGCTC 1 cut(s) 413
Eco130I CCWWGG 1 cut(s) 90
Eco24I GRGCYC 1 cut(s) 415
Eco47I GGWCC 3 cut(s) 233, 599, 705
Eco53kI GAGCTC 1 cut(s) 413
Eco57I CTGAAG 1 cut(s) 249
EcoICRI GAGCTC 1 cut(s) 413
EcoO109I RGGNCCY 2 cut(s) 599, 705
EcoRI GAATTC 1 cut(s) 279
EcoRII CCWGG 1 cut(s) 353
EcoT14I CCWWGG 1 cut(s) 90
EcoT38I GRGCYC 1 cut(s) 415
EgeI GGCGCC 1 cut(s) 465
EheI GGCGCC 1 cut(s) 465
ErhI CCWWGG 1 cut(s) 90
Esp3I CGTCTC 1 cut(s) 473
FauNDI CATATG 1 cut(s) 805
FblI GTMKAC 1 cut(s) 613
Fnu4HI GCNGC 1 cut(s) 390
FokI GGATG 1 cut(s) 338
FriOI GRGCYC 1 cut(s) 415
Fsp4HI GCNGC 1 cut(s) 390
FspBI CTAG 2 cut(s) 50, 677
GlaI GCGC 1 cut(s) 465
GluI GCNGC 1 cut(s) 390
HaeII RGCGCY 1 cut(s) 467
HaeIII GGCC 1 cut(s) 743
HapII CCGG 2 cut(s) 135, 503
HhaI GCGC 1 cut(s) 466
Hin1I GRCGYC 1 cut(s) 464
Hin6I GCGC 1 cut(s) 464
HinP1I GCGC 1 cut(s) 464
HincII GTYRAC 1 cut(s) 256
HindII GTYRAC 1 cut(s) 256
HinfI GANTC 7 cut(s) 44, 82, 114, 181, 375, 534, 670
HpaI GTTAAC 1 cut(s) 256
HpaII CCGG 2 cut(s) 135, 503
HphI GGTGA 1 cut(s) 706
Hpy166II GTNNAC 3 cut(s) 201, 256, 614
Hpy188I TCNGA 5 cut(s) 180, 246, 478, 539, 624
Hpy188III TCNNGA 4 cut(s) 372, 503, 647, 703
Hpy8I GTNNAC 3 cut(s) 201, 256, 614
HpyAV CCTTC 1 cut(s) 743
HpyCH4III ACNGT 2 cut(s) 558, 684
HpyCH4V TGCA 5 cut(s) 329, 347, 389, 572, 758
HpyF3I CTNAG 8 cut(s) 214, 245, 404, 421, 477, 536, 701, 816
Hsp92I GRCGYC 1 cut(s) 464
HspAI GCGC 1 cut(s) 464
KasI GGCGCC 1 cut(s) 463
Kpn2I TCCGGA 1 cut(s) 502
KspAI GTTAAC 1 cut(s) 256
Kzo9I GATC 3 cut(s) 379, 525, 643
LguI GCTCTTC 1 cut(s) 403
LmnI GCTCC 1 cut(s) 223
Lsp1109I GCAGC 1 cut(s) 401
LweI GCATC 1 cut(s) 316
MaeI CTAG 2 cut(s) 50, 677
MaeIII GTNAC 4 cut(s) 79, 399, 424, 712
MalI GATC 3 cut(s) 381, 527, 645
MboI GATC 3 cut(s) 379, 525, 643
MboII GAAGA 5 cut(s) 165, 374, 420, 589, 791
MhlI GDGCHC 1 cut(s) 415
MluCI AATT 8 cut(s) 28, 240, 279, 468, 546, 562, 662, 745
Mly113I GGCGCC 1 cut(s) 464
MlyI GAGTC 3 cut(s) 76, 175, 528
MnlI CCTC 6 cut(s) 114, 216, 266, 480, 510, 618
MroI TCCGGA 1 cut(s) 502
MroXI GAANNNNTTC 1 cut(s) 443
MseI TTAA 3 cut(s) 192, 255, 285
MspA1I CMGCKG 1 cut(s) 230
MspI CCGG 2 cut(s) 135, 503
MspR9I CCNGG 2 cut(s) 136, 355
MvaI CCWGG 1 cut(s) 355
NarI GGCGCC 1 cut(s) 464
NciI CCSGG 1 cut(s) 136
NdeI CATATG 1 cut(s) 805
NdeII GATC 3 cut(s) 379, 525, 643
NlaIV GGNNCC 2 cut(s) 465, 707
NmuCI GTSAC 2 cut(s) 79, 712
NspV TTCGAA 1 cut(s) 668
PciSI GCTCTTC 1 cut(s) 403
PdmI GAANNNNTTC 1 cut(s) 443
PfeI GAWTC 4 cut(s) 44, 114, 375, 670
PkrI GCNGC 1 cut(s) 391
PleI GAGTC 3 cut(s) 76, 175, 528
PluTI GGCGCC 1 cut(s) 467
PpsI GAGTC 3 cut(s) 76, 175, 528
PpuMI RGGWCCY 2 cut(s) 599, 705
PshAI GACNNNNGTC 1 cut(s) 710
Psp124BI GAGCTC 1 cut(s) 415
Psp5II RGGWCCY 2 cut(s) 599, 705
Psp6I CCWGG 1 cut(s) 353
PspGI CCWGG 1 cut(s) 353
PspN4I GGNNCC 2 cut(s) 465, 707
PspPI GGNCC 3 cut(s) 233, 599, 705
PspPPI RGGWCCY 2 cut(s) 599, 705
PstI CTGCAG 1 cut(s) 391
PvuII CAGCTG 1 cut(s) 230
RsaI GTAC 1 cut(s) 560
RsaNI GTAC 1 cut(s) 559
SacI GAGCTC 1 cut(s) 415
SapI GCTCTTC 1 cut(s) 403
SaqAI TTAA 3 cut(s) 192, 255, 285
SatI GCNGC 1 cut(s) 390
Sau3AI GATC 3 cut(s) 379, 525, 643
Sau96I GGNCC 3 cut(s) 233, 599, 705
SchI GAGTC 3 cut(s) 76, 175, 528
ScrFI CCNGG 2 cut(s) 136, 355
SduI GDGCHC 1 cut(s) 415
SfaNI GCATC 1 cut(s) 316
SfcI CTRYAG 1 cut(s) 387
SfoI GGCGCC 1 cut(s) 465
SfuI TTCGAA 1 cut(s) 668
SinI GGWCC 3 cut(s) 233, 599, 705
SmlI CTYRAG 1 cut(s) 370
SmoI CTYRAG 1 cut(s) 370
Sse9I AATT 8 cut(s) 28, 240, 279, 468, 546, 562, 662, 745
SspDI GGCGCC 1 cut(s) 463
SspI AATATT 1 cut(s) 303
SspMI CTAG 2 cut(s) 50, 677
SstI GAGCTC 1 cut(s) 415
StyD4I CCNGG 2 cut(s) 134, 353
StyI CCWWGG 1 cut(s) 90
TaaI ACNGT 2 cut(s) 558, 684
TaqI TCGA 5 cut(s) 117, 378, 437, 494, 668
TasI AATT 8 cut(s) 28, 240, 279, 468, 546, 562, 662, 745
TatI WGTACW 1 cut(s) 558
TfiI GAWTC 4 cut(s) 44, 114, 375, 670
Tru1I TTAA 3 cut(s) 192, 255, 285
Tru9I TTAA 3 cut(s) 192, 255, 285
TseFI GTSAC 2 cut(s) 79, 712
TseI GCWGC 1 cut(s) 389
Tsp45I GTSAC 2 cut(s) 79, 712
TspDTI ATGAA 2 cut(s) 303, 436
TspGWI ACGGA 1 cut(s) 497
VpaK11BI GGWCC 3 cut(s) 233, 599, 705
XapI RAATTY 2 cut(s) 279, 662
XmiI GTMKAC 1 cut(s) 613
XmnI GAANNNNTTC 1 cut(s) 443
XspI CTAG 2 cut(s) 50, 677
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.