RchiOBHm_Chr4g0425441

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0001879
Physical Location & Seq
Forward (+)
51023914 .. 51024759
846 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ39459

Sequence Viewer

Length: 561 bp
ATGGAGCCAGCTATAAAGCTACTTAGAAGTGCTCTGTTCTCAGTAACAATCGGCTCGAATGATTTCATCAATAACTACTTGGCGCCAGTTATATCTGAGGCTGAGAGGAAACTCAGACTACAAATTACAGACCTATTTTGGGAAGTAAATCAAGCTGATCCAGAGAGCTGTTCGGAATTTTCGAATCAACTAGCCCAGTCATTCAATGGCAAGCTAAGGATCCTTGTCCCCGAGCTAAACAAGAACCTTCCTGGAGTCAATTTTGTGTATGCAGATATTCATAGTATTGTGGAAGACATTATTGAGACTACATATCATATGGGGAGACTTGGTTTCGAGAATACAAACCCTGCATGCTGCAGAGTAGCAGGGCGCTACGGAGGTCTAATTCCATGCGGTCCTCAGCCATCAAAAGTTTGCGTGGACCGGTCGAAGTATATGTTTTGGGATCCTTACCATCCTTCTGATGCTTCTAATACGATCATAGCAAGGCGTCTCTTGTATGGGAACTCCAGTGATATTTCACCCATGAATGTACTGCAACTCCTCCAAGCTTCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

186

Amino Acids

20.81

Weight (kDa)

5.6

Isoelectric Point (pI)

50.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 11 - 166 1.9e-13 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 82
AciI CCGC 1 cut(s) 396
AclWI GGATC 5 cut(s) 152, 214, 227, 443, 456
AcsI RAATTY 1 cut(s) 176
AcyI GRCGYC 2 cut(s) 83, 493
AfaI GTAC 1 cut(s) 537
AfiI CCNNNNNNNGG 1 cut(s) 139
AgeI ACCGGT 1 cut(s) 426
AgsI TTSAA 1 cut(s) 205
AjnI CCWGG 1 cut(s) 250
AluBI AGCT 7 cut(s) 11, 19, 155, 168, 214, 235, 554
AluI AGCT 7 cut(s) 11, 19, 155, 168, 214, 235, 554
Alw21I GWGCWC 1 cut(s) 34
Alw26I GTCTC 3 cut(s) 299, 319, 500
AlwI GGATC 5 cut(s) 152, 214, 227, 443, 456
Ama87I CYCGRG 1 cut(s) 230
ApeKI GCWGC 1 cut(s) 357
ApoI RAATTY 1 cut(s) 176
AsiGI ACCGGT 1 cut(s) 426
Asp700I GAANNNNTTC 1 cut(s) 62
AspLEI GCGC 2 cut(s) 85, 375
AspS9I GGNCC 2 cut(s) 398, 424
AsuHPI GGTGA 1 cut(s) 516
AsuII TTCGAA 1 cut(s) 182
AvaI CYCGRG 1 cut(s) 230
AvaII GGWCC 2 cut(s) 398, 424
BamHI GGATCC 2 cut(s) 219, 448
BanI GGYRCC 1 cut(s) 82
BbsI GAAGAC 1 cut(s) 300
Bbv12I GWGCWC 1 cut(s) 34
BbvCI CCTCAGC 1 cut(s) 402
BbvI GCAGC 1 cut(s) 344
BccI CCATC 2 cut(s) 415, 465
BciT130I CCWGG 1 cut(s) 252
BcoDI GTCTC 3 cut(s) 299, 319, 500
BfaI CTAG 1 cut(s) 191
BfmI CTRYAG 1 cut(s) 358
BfoI RGCGCY 2 cut(s) 86, 376
BisI GCNGC 1 cut(s) 358
BlsI GCNGC 1 cut(s) 359
Bme1390I CCNGG 1 cut(s) 252
Bme18I GGWCC 2 cut(s) 398, 424
BmeT110I CYCGRG 1 cut(s) 230
BmgT120I GGNCC 2 cut(s) 398, 424
BmiI GGNNCC 4 cut(s) 6, 84, 221, 450
BmrFI CCNGG 1 cut(s) 252
BmrI ACTGGG 1 cut(s) 190
BmsI GCATC 1 cut(s) 457
BmuI ACTGGG 1 cut(s) 190
BpiI GAAGAC 1 cut(s) 300
BpmI CTGGAG 2 cut(s) 273, 496
Bpu10I CCTNAGC 2 cut(s) 215, 402
Bpu14I TTCGAA 1 cut(s) 182
BsaHI GRCGYC 2 cut(s) 83, 493
BsaWI WCCGGW 1 cut(s) 426
BsaXI ACNNNNNCTCC 4 cut(s) 316, 346, 528, 558
Bsc4I CCNNNNNNNGG 1 cut(s) 139
Bse118I RCCGGY 1 cut(s) 426
Bse1I ACTGG 3 cut(s) 86, 196, 513
BseBI CCWGG 1 cut(s) 252
BseGI GGATG 1 cut(s) 457
BseLI CCNNNNNNNGG 1 cut(s) 139
BseMII CTCAG 5 cut(s) 54, 87, 93, 127, 416
BseNI ACTGG 3 cut(s) 86, 196, 513
BseRI GAGGAG 1 cut(s) 536
BseXI GCAGC 1 cut(s) 344
Bsh1285I CGRYCG 1 cut(s) 431
BshNI GGYRCC 1 cut(s) 82
BshTI ACCGGT 1 cut(s) 426
BsiEI CGRYCG 1 cut(s) 431
BsiHKAI GWGCWC 1 cut(s) 34
BsiHKCI CYCGRG 1 cut(s) 230
BsiSI CCGG 1 cut(s) 427
BslFI GGGAC 1 cut(s) 212
BslI CCNNNNNNNGG 1 cut(s) 139
BsmAI GTCTC 3 cut(s) 299, 319, 500
BsmBI CGTCTC 1 cut(s) 500
BsmFI GGGAC 1 cut(s) 212
BsoBI CYCGRG 1 cut(s) 230
Bsp119I TTCGAA 1 cut(s) 182
Bsp1286I GDGCHC 1 cut(s) 34
Bsp143I GATC 4 cut(s) 157, 219, 448, 480
BspACI CCGC 1 cut(s) 396
BspCNI CTCAG 5 cut(s) 53, 88, 94, 126, 415
BspLI GGNNCC 4 cut(s) 6, 84, 221, 450
BspMAI CTGCAG 1 cut(s) 362
BspPI GGATC 5 cut(s) 152, 214, 227, 443, 456
BspT104I TTCGAA 1 cut(s) 182
BspT107I GGYRCC 1 cut(s) 82
BsrFI RCCGGY 1 cut(s) 426
BsrI ACTGG 3 cut(s) 86, 196, 513
BssAI RCCGGY 1 cut(s) 426
BssMI GATC 4 cut(s) 157, 219, 448, 480
BssNI GRCGYC 2 cut(s) 83, 493
Bst2UI CCWGG 1 cut(s) 252
BstACI GRCGYC 2 cut(s) 83, 493
BstBI TTCGAA 1 cut(s) 182
BstC8I GCNNGC 3 cut(s) 9, 212, 355
BstDEI CTNAG 7 cut(s) 23, 40, 96, 102, 113, 215, 402
BstF5I GGATG 1 cut(s) 457
BstH2I RGCGCY 2 cut(s) 86, 376
BstHHI GCGC 2 cut(s) 85, 375
BstKTI GATC 4 cut(s) 160, 222, 451, 483
BstMAI GTCTC 3 cut(s) 299, 319, 500
BstMBI GATC 4 cut(s) 157, 219, 448, 480
BstMCI CGRYCG 1 cut(s) 431
BstNI CCWGG 1 cut(s) 252
BstNSI RCATGY 1 cut(s) 357
BstSCI CCNGG 1 cut(s) 250
BstSFI CTRYAG 1 cut(s) 358
BstV1I GCAGC 1 cut(s) 344
BstV2I GAAGAC 1 cut(s) 300
BstX2I RGATCY 2 cut(s) 219, 448
BstYI RGATCY 2 cut(s) 219, 448
BtsCI GGATG 1 cut(s) 457
BtsIMutI CAGTG 1 cut(s) 520
Cac8I GCNNGC 3 cut(s) 9, 212, 355
CfoI GCGC 2 cut(s) 85, 375
Cfr10I RCCGGY 1 cut(s) 426
Cfr13I GGNCC 2 cut(s) 398, 424
CseI GACGC 1 cut(s) 482
Csp6I GTAC 1 cut(s) 536
CspAI ACCGGT 1 cut(s) 426
CviAII CATG 3 cut(s) 354, 393, 529
CviQI GTAC 1 cut(s) 536
DdeI CTNAG 7 cut(s) 23, 40, 96, 102, 113, 215, 402
DinI GGCGCC 1 cut(s) 84
DpnI GATC 4 cut(s) 159, 221, 450, 482
DpnII GATC 4 cut(s) 157, 219, 448, 480
Eco47I GGWCC 2 cut(s) 398, 424
Eco88I CYCGRG 1 cut(s) 230
EcoRII CCWGG 1 cut(s) 250
EgeI GGCGCC 1 cut(s) 84
EheI GGCGCC 1 cut(s) 84
Esp3I CGTCTC 1 cut(s) 500
FaeI CATG 3 cut(s) 357, 396, 532
FaqI GGGAC 1 cut(s) 212
FatI CATG 3 cut(s) 353, 392, 528
FauNDI CATATG 1 cut(s) 318
Fnu4HI GCNGC 1 cut(s) 358
FokI GGATG 1 cut(s) 444
Fsp4HI GCNGC 1 cut(s) 358
FspBI CTAG 1 cut(s) 191
GlaI GCGC 2 cut(s) 84, 374
GluI GCNGC 1 cut(s) 358
GsuI CTGGAG 2 cut(s) 273, 496
HaeII RGCGCY 2 cut(s) 86, 376
HapII CCGG 1 cut(s) 427
HgaI GACGC 1 cut(s) 482
HhaI GCGC 2 cut(s) 85, 375
Hin1I GRCGYC 2 cut(s) 83, 493
Hin1II CATG 3 cut(s) 357, 396, 532
Hin6I GCGC 2 cut(s) 83, 373
HinP1I GCGC 2 cut(s) 83, 373
HindIII AAGCTT 1 cut(s) 552
HinfI GANTC 2 cut(s) 184, 255
HpaII CCGG 1 cut(s) 427
HphI GGTGA 1 cut(s) 516
Hpy166II GTNNAC 1 cut(s) 424
Hpy188I TCNGA 4 cut(s) 97, 116, 175, 466
Hpy188III TCNNGA 3 cut(s) 161, 337, 558
Hpy8I GTNNAC 1 cut(s) 424
HpyAV CCTTC 2 cut(s) 257, 471
HpyCH4V TGCA 4 cut(s) 272, 353, 360, 541
HpyF3I CTNAG 7 cut(s) 23, 40, 96, 102, 113, 215, 402
Hsp92I GRCGYC 2 cut(s) 83, 493
Hsp92II CATG 3 cut(s) 357, 396, 532
HspAI GCGC 2 cut(s) 83, 373
KasI GGCGCC 1 cut(s) 82
Kzo9I GATC 4 cut(s) 157, 219, 448, 480
LmnI GCTCC 1 cut(s) 4
Lsp1109I GCAGC 1 cut(s) 344
LweI GCATC 1 cut(s) 457
MaeI CTAG 1 cut(s) 191
MaeIII GTNAC 1 cut(s) 43
MalI GATC 4 cut(s) 159, 221, 450, 482
MboI GATC 4 cut(s) 157, 219, 448, 480
MboII GAAGA 1 cut(s) 305
MflI RGATCY 2 cut(s) 219, 448
MhlI GDGCHC 1 cut(s) 34
MluCI AATT 4 cut(s) 123, 176, 259, 387
Mly113I GGCGCC 1 cut(s) 83
MlyI GAGTC 1 cut(s) 264
MnlI CCTC 5 cut(s) 91, 99, 374, 411, 557
MroXI GAANNNNTTC 1 cut(s) 62
MspI CCGG 1 cut(s) 427
MspR9I CCNGG 1 cut(s) 252
MvaI CCWGG 1 cut(s) 252
NarI GGCGCC 1 cut(s) 83
NdeI CATATG 1 cut(s) 318
NdeII GATC 4 cut(s) 157, 219, 448, 480
NlaIII CATG 3 cut(s) 357, 396, 532
NlaIV GGNNCC 4 cut(s) 6, 84, 221, 450
NspI RCATGY 1 cut(s) 357
NspV TTCGAA 1 cut(s) 182
PaeI GCATGC 1 cut(s) 357
PcsI WCGNNNNNNNCGW 1 cut(s) 179
PdmI GAANNNNTTC 1 cut(s) 62
PfeI GAWTC 1 cut(s) 184
PfoI TCCNGGA 1 cut(s) 250
PinAI ACCGGT 1 cut(s) 426
PkrI GCNGC 1 cut(s) 359
PleI GAGTC 1 cut(s) 263
PluTI GGCGCC 1 cut(s) 86
PpsI GAGTC 1 cut(s) 263
Psp6I CCWGG 1 cut(s) 250
PspGI CCWGG 1 cut(s) 250
PspN4I GGNNCC 4 cut(s) 6, 84, 221, 450
PspPI GGNCC 2 cut(s) 398, 424
PstI CTGCAG 1 cut(s) 362
PsuI RGATCY 2 cut(s) 219, 448
RsaI GTAC 1 cut(s) 537
RsaNI GTAC 1 cut(s) 536
SatI GCNGC 1 cut(s) 358
Sau3AI GATC 4 cut(s) 157, 219, 448, 480
Sau96I GGNCC 2 cut(s) 398, 424
SchI GAGTC 1 cut(s) 264
ScrFI CCNGG 1 cut(s) 252
SduI GDGCHC 1 cut(s) 34
SfaNI GCATC 1 cut(s) 457
SfcI CTRYAG 1 cut(s) 358
SfoI GGCGCC 1 cut(s) 84
SfuI TTCGAA 1 cut(s) 182
SinI GGWCC 2 cut(s) 398, 424
SphI GCATGC 1 cut(s) 357
Sse9I AATT 4 cut(s) 123, 176, 259, 387
SsiI CCGC 1 cut(s) 396
SspDI GGCGCC 1 cut(s) 82
SspMI CTAG 1 cut(s) 191
StyD4I CCNGG 1 cut(s) 250
TaqI TCGA 4 cut(s) 56, 182, 336, 431
TasI AATT 4 cut(s) 123, 176, 259, 387
TatI WGTACW 1 cut(s) 535
TfiI GAWTC 1 cut(s) 184
TscAI CASTG 1 cut(s) 520
TseI GCWGC 1 cut(s) 357
TspDTI ATGAA 3 cut(s) 55, 269, 545
TspGWI ACGGA 1 cut(s) 393
TspRI CASTG 1 cut(s) 520
VpaK11BI GGWCC 2 cut(s) 398, 424
XapI RAATTY 1 cut(s) 176
XceI RCATGY 1 cut(s) 357
XcmI CCANNNNNNNNNTGG 1 cut(s) 203
XmnI GAANNNNTTC 1 cut(s) 62
XspI CTAG 1 cut(s) 191
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.