RLG00000007351

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr2
OG0001879
Physical Location & Seq
Reverse (-)
16068392 .. 16070995
2604 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000007351

Sequence Viewer

Length: 972 bp
ATGCCTGCAAATCTGCAACTGCAAGAAAGTATACTACTCTCAGCTTTAGGGCCTATAAATGTAGATATTGACAAGCACAAGTTTCTCTACAAAGACCTAAAAAGTATAGGTCAGACTCTTGGTTTTAAGGGGTTCACTCCTCCATACTTAGCTCCCACAACAGCTGGACCAGTAATTCTGAGGGGTGTTAACTATGCTTCAGGTGGAGGTGGAATTCTTAATGAAACAGGAAAAATATTTGTTGGTAGAATAAACTTGGATGCACAGATAGATAACTTTGCAAATACCAGGCAAGACATTATCTCAAGAATCGGTCTTCCTGCCGCTCTAAAGTTACTTAGAAGAGCTCTGTTCTCAGTAACAATCGGCTCGAATGATTTCATCAATAACTACTTGGCGCCAGTTATATCTGAGACCGAGAGGAAGTTGATACCTCCGGAAACCTTTGTTGGCATTTTGATCGCAAGACTCAGACTACAAATTACGAGACTGTACAATTTGGGTGCAAGGAAGATGGTTGTGGTAAATGTAGGACCTATTGGGTGTATACCTTATCAGAGGGAAGTAAATCAAGCTGATCCAGAGAGCTGTGCGGAATTTTCGAATCAACTAGCACGGTCATTCAATGCCAAGCTAAGGACCCTTGTCACCGAGCTAAACAAGAACCTTCCTACGGCCAATTTTGTTTATGCAGATGTTTATAGTATTTTCGAAGAAATTATTGAGAACTACATATCATATGGTTTCGAGAATGCAAACTCTGCATGCTGCAGAGTAGCAGGGCGCTATGGGGGTCTAATTCCATGCGGTCCTCAGCCATCAAAAGTTTGCGTGGACCGGTCAAAGTATGTGTTTTGGGATGCTTACCATCCTTCTGATGCTTCTAATATGATCATAGCATGGCGTCTCTTGTATGGGAACTCCAGTGATATTTCACCCATGAATGTAGTGCAACTTCTCCAAGCTTCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

324

Amino Acids

35.58

Weight (kDa)

8.81

Isoelectric Point (pI)

40.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 54 - 300 7.8e-26 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 397
AccBSI CCGCTC 1 cut(s) 326
AccI GTMKAC 2 cut(s) 31, 547
AccIII TCCGGA 1 cut(s) 436
AciI CCGC 3 cut(s) 324, 593, 807
AclWI GGATC 1 cut(s) 572
AcoI YGGCCR 1 cut(s) 675
AcsI RAATTY 2 cut(s) 213, 596
AcuI CTGAAG 1 cut(s) 183
AcyI GRCGYC 2 cut(s) 398, 904
AfaI GTAC 1 cut(s) 494
AfiI CCNNNNNNNGG 2 cut(s) 636, 673
AgeI ACCGGT 1 cut(s) 837
AgsI TTSAA 1 cut(s) 625
AjnI CCWGG 1 cut(s) 287
AjuI GAANNNNNNNTTGG 2 cut(s) 432, 464
AluBI AGCT 9 cut(s) 44, 152, 164, 347, 575, 588, 634, 655, 965
AluI AGCT 9 cut(s) 44, 152, 164, 347, 575, 588, 634, 655, 965
Alw21I GWGCWC 1 cut(s) 349
Alw26I GTCTC 3 cut(s) 407, 481, 911
AlwI GGATC 1 cut(s) 572
Aor13HI TCCGGA 1 cut(s) 436
AoxI GGCC 2 cut(s) 50, 675
ApeKI GCWGC 1 cut(s) 768
ApoI RAATTY 2 cut(s) 213, 596
AsiGI ACCGGT 1 cut(s) 837
Asp700I GAANNNNTTC 1 cut(s) 377
AspLEI GCGC 2 cut(s) 400, 786
AspS9I GGNCC 6 cut(s) 50, 167, 533, 639, 809, 835
AsuHPI GGTGA 2 cut(s) 640, 927
AsuII TTCGAA 2 cut(s) 602, 711
AvaII GGWCC 5 cut(s) 167, 533, 639, 809, 835
BanI GGYRCC 1 cut(s) 397
BanII GRGCYC 1 cut(s) 349
BarI GAAGNNNNNNTAC 2 cut(s) 939, 971
BbsI GAAGAC 1 cut(s) 308
Bbv12I GWGCWC 1 cut(s) 349
BbvCI CCTCAGC 1 cut(s) 813
BbvI GCAGC 1 cut(s) 755
BccI CCATC 3 cut(s) 508, 826, 876
BceAI ACGGC 1 cut(s) 690
BcgI CGANNNNNNTGC 2 cut(s) 442, 476
BciT130I CCWGG 1 cut(s) 289
BclI TGATCA 1 cut(s) 891
BcoDI GTCTC 3 cut(s) 407, 481, 911
BfaI CTAG 1 cut(s) 611
BfmI CTRYAG 1 cut(s) 769
BfoI RGCGCY 2 cut(s) 401, 787
BisI GCNGC 2 cut(s) 324, 769
BlsI GCNGC 2 cut(s) 325, 770
Bme1390I CCNGG 1 cut(s) 289
Bme18I GGWCC 5 cut(s) 167, 533, 639, 809, 835
BmgT120I GGNCC 6 cut(s) 50, 167, 533, 639, 809, 835
BmiI GGNNCC 2 cut(s) 399, 641
BmrFI CCNGG 1 cut(s) 289
BmsI GCATC 3 cut(s) 250, 850, 868
BoxI GACNNNNGTC 1 cut(s) 644
BpiI GAAGAC 1 cut(s) 308
BpmI CTGGAG 1 cut(s) 907
Bpu10I CCTNAGC 2 cut(s) 635, 813
Bpu14I TTCGAA 2 cut(s) 602, 711
BpuEI CTTGAG 1 cut(s) 289
BsaHI GRCGYC 2 cut(s) 398, 904
BsaI GGTCTC 1 cut(s) 407
BsaWI WCCGGW 2 cut(s) 436, 837
Bsc4I CCNNNNNNNGG 2 cut(s) 636, 673
Bse118I RCCGGY 1 cut(s) 837
Bse1I ACTGG 3 cut(s) 170, 401, 924
BseAI TCCGGA 1 cut(s) 436
BseBI CCWGG 1 cut(s) 289
BseGI GGATG 3 cut(s) 265, 865, 868
BseLI CCNNNNNNNGG 2 cut(s) 636, 673
BseMII CTCAG 6 cut(s) 54, 170, 369, 402, 484, 827
BseNI ACTGG 3 cut(s) 170, 401, 924
BseRI GAGGAG 1 cut(s) 129
BseXI GCAGC 1 cut(s) 755
BshFI GGCC 2 cut(s) 52, 677
BshNI GGYRCC 1 cut(s) 397
BshTI ACCGGT 1 cut(s) 837
BsiHKAI GWGCWC 1 cut(s) 349
BsiSI CCGG 2 cut(s) 437, 838
BslI CCNNNNNNNGG 2 cut(s) 636, 673
BsmAI GTCTC 3 cut(s) 407, 481, 911
BsmBI CGTCTC 1 cut(s) 911
BsmI GAATGC 1 cut(s) 757
BsnI GGCC 2 cut(s) 52, 677
Bso31I GGTCTC 1 cut(s) 407
Bsp119I TTCGAA 2 cut(s) 602, 711
Bsp1286I GDGCHC 1 cut(s) 349
Bsp13I TCCGGA 1 cut(s) 436
Bsp1407I TGTACA 1 cut(s) 492
Bsp143I GATC 3 cut(s) 459, 577, 891
BspACI CCGC 3 cut(s) 324, 593, 807
BspANI GGCC 2 cut(s) 52, 677
BspCNI CTCAG 6 cut(s) 53, 171, 368, 403, 483, 826
BspEI TCCGGA 1 cut(s) 436
BspLI GGNNCC 2 cut(s) 399, 641
BspMAI CTGCAG 1 cut(s) 773
BspPI GGATC 1 cut(s) 572
BspQI GCTCTTC 1 cut(s) 337
BspT104I TTCGAA 2 cut(s) 602, 711
BspT107I GGYRCC 1 cut(s) 397
BspTNI GGTCTC 1 cut(s) 407
BsrBI CCGCTC 1 cut(s) 326
BsrFI RCCGGY 1 cut(s) 837
BsrGI TGTACA 1 cut(s) 492
BsrI ACTGG 3 cut(s) 170, 401, 924
BssAI RCCGGY 1 cut(s) 837
BssMI GATC 3 cut(s) 459, 577, 891
BssNAI GTATAC 2 cut(s) 32, 548
BssNI GRCGYC 2 cut(s) 398, 904
Bst1107I GTATAC 2 cut(s) 32, 548
Bst2UI CCWGG 1 cut(s) 289
Bst4CI ACNGT 2 cut(s) 492, 618
Bst6I CTCTTC 1 cut(s) 337
BstACI GRCGYC 2 cut(s) 398, 904
BstAPI GCANNNNNTGC 1 cut(s) 761
BstAUI TGTACA 1 cut(s) 492
BstBI TTCGAA 2 cut(s) 602, 711
BstC8I GCNNGC 2 cut(s) 6, 766
BstDEI CTNAG 9 cut(s) 40, 148, 179, 338, 355, 411, 470, 635, 813
BstF5I GGATG 3 cut(s) 265, 865, 868
BstH2I RGCGCY 2 cut(s) 401, 787
BstHHI GCGC 2 cut(s) 400, 786
BstKTI GATC 3 cut(s) 462, 580, 894
BstMAI GTCTC 3 cut(s) 407, 481, 911
BstMBI GATC 3 cut(s) 459, 577, 891
BstMWI GCNNNNNNNGC 1 cut(s) 761
BstNI CCWGG 1 cut(s) 289
BstNSI RCATGY 1 cut(s) 768
BstPAI GACNNNNGTC 1 cut(s) 644
BstSCI CCNGG 1 cut(s) 287
BstSFI CTRYAG 1 cut(s) 769
BstV1I GCAGC 1 cut(s) 755
BstV2I GAAGAC 1 cut(s) 308
BstZ17I GTATAC 2 cut(s) 32, 548
BsuRI GGCC 2 cut(s) 52, 677
BtsCI GGATG 3 cut(s) 265, 865, 868
BtsIMutI CAGTG 1 cut(s) 931
Cac8I GCNNGC 2 cut(s) 6, 766
CfoI GCGC 2 cut(s) 400, 786
Cfr10I RCCGGY 1 cut(s) 837
Cfr13I GGNCC 6 cut(s) 50, 167, 533, 639, 809, 835
CseI GACGC 1 cut(s) 893
Csp6I GTAC 1 cut(s) 493
CspAI ACCGGT 1 cut(s) 837
CviAII CATG 4 cut(s) 765, 804, 900, 940
CviQI GTAC 1 cut(s) 493
DdeI CTNAG 9 cut(s) 40, 148, 179, 338, 355, 411, 470, 635, 813
DinI GGCGCC 1 cut(s) 399
DpnI GATC 3 cut(s) 461, 579, 893
DpnII GATC 3 cut(s) 459, 577, 891
EaeI YGGCCR 1 cut(s) 675
Eam1104I CTCTTC 1 cut(s) 337
EarI CTCTTC 1 cut(s) 337
Ecl136II GAGCTC 1 cut(s) 347
Eco24I GRGCYC 1 cut(s) 349
Eco31I GGTCTC 1 cut(s) 407
Eco47I GGWCC 5 cut(s) 167, 533, 639, 809, 835
Eco53kI GAGCTC 1 cut(s) 347
Eco57I CTGAAG 1 cut(s) 183
EcoICRI GAGCTC 1 cut(s) 347
EcoO109I RGGNCCY 3 cut(s) 50, 533, 639
EcoRI GAATTC 1 cut(s) 213
EcoRII CCWGG 1 cut(s) 287
EcoT38I GRGCYC 1 cut(s) 349
EgeI GGCGCC 1 cut(s) 399
EheI GGCGCC 1 cut(s) 399
Esp3I CGTCTC 1 cut(s) 911
FaeI CATG 4 cut(s) 768, 807, 903, 943
FatI CATG 4 cut(s) 764, 803, 899, 939
FauNDI CATATG 1 cut(s) 739
FbaI TGATCA 1 cut(s) 891
FblI GTMKAC 2 cut(s) 31, 547
Fnu4HI GCNGC 2 cut(s) 324, 769
FokI GGATG 3 cut(s) 272, 855, 872
FriOI GRGCYC 1 cut(s) 349
Fsp4HI GCNGC 2 cut(s) 324, 769
FspBI CTAG 1 cut(s) 611
GlaI GCGC 2 cut(s) 399, 785
GluI GCNGC 2 cut(s) 324, 769
GsuI CTGGAG 1 cut(s) 907
HaeII RGCGCY 2 cut(s) 401, 787
HaeIII GGCC 2 cut(s) 52, 677
HapII CCGG 2 cut(s) 437, 838
HgaI GACGC 1 cut(s) 893
HhaI GCGC 2 cut(s) 400, 786
Hin1I GRCGYC 2 cut(s) 398, 904
Hin1II CATG 4 cut(s) 768, 807, 903, 943
Hin6I GCGC 2 cut(s) 398, 784
HinP1I GCGC 2 cut(s) 398, 784
HincII GTYRAC 1 cut(s) 190
HindII GTYRAC 1 cut(s) 190
HindIII AAGCTT 1 cut(s) 963
HinfI GANTC 4 cut(s) 115, 309, 468, 604
HpaI GTTAAC 1 cut(s) 190
HpaII CCGG 2 cut(s) 437, 838
HphI GGTGA 2 cut(s) 640, 927
Hpy166II GTNNAC 5 cut(s) 32, 135, 190, 548, 835
Hpy188I TCNGA 6 cut(s) 114, 180, 412, 473, 558, 877
Hpy188III TCNNGA 5 cut(s) 306, 437, 581, 748, 969
Hpy8I GTNNAC 5 cut(s) 32, 135, 190, 548, 835
HpyAV CCTTC 2 cut(s) 677, 882
HpyCH4III ACNGT 2 cut(s) 492, 618
HpyF10VI GCNNNNNNNGC 1 cut(s) 761
HpyF3I CTNAG 9 cut(s) 40, 148, 179, 338, 355, 411, 470, 635, 813
Hsp92I GRCGYC 2 cut(s) 398, 904
Hsp92II CATG 4 cut(s) 768, 807, 903, 943
HspAI GCGC 2 cut(s) 398, 784
KasI GGCGCC 1 cut(s) 397
Kpn2I TCCGGA 1 cut(s) 436
Ksp22I TGATCA 1 cut(s) 891
KspAI GTTAAC 1 cut(s) 190
Kzo9I GATC 3 cut(s) 459, 577, 891
LguI GCTCTTC 1 cut(s) 337
LmnI GCTCC 1 cut(s) 157
Lsp1109I GCAGC 1 cut(s) 755
LweI GCATC 3 cut(s) 250, 850, 868
MaeI CTAG 1 cut(s) 611
MaeIII GTNAC 3 cut(s) 333, 358, 646
MalI GATC 3 cut(s) 461, 579, 893
MbiI CCGCTC 1 cut(s) 326
MboI GATC 3 cut(s) 459, 577, 891
MboII GAAGA 4 cut(s) 308, 354, 523, 725
MhlI GDGCHC 1 cut(s) 349
MluCI AATT 8 cut(s) 174, 213, 480, 496, 596, 679, 717, 798
Mly113I GGCGCC 1 cut(s) 398
MlyI GAGTC 2 cut(s) 109, 462
MnlI CCTC 7 cut(s) 150, 174, 200, 414, 444, 552, 822
MroI TCCGGA 1 cut(s) 436
MroXI GAANNNNTTC 1 cut(s) 377
MseI TTAA 3 cut(s) 126, 189, 219
MspA1I CMGCKG 1 cut(s) 164
MspI CCGG 2 cut(s) 437, 838
MspR9I CCNGG 1 cut(s) 289
Mva1269I GAATGC 1 cut(s) 757
MvaI CCWGG 1 cut(s) 289
MwoI GCNNNNNNNGC 1 cut(s) 761
NarI GGCGCC 1 cut(s) 398
NdeI CATATG 1 cut(s) 739
NdeII GATC 3 cut(s) 459, 577, 891
NlaIII CATG 4 cut(s) 768, 807, 903, 943
NlaIV GGNNCC 2 cut(s) 399, 641
NmuCI GTSAC 1 cut(s) 646
NspI RCATGY 1 cut(s) 768
NspV TTCGAA 2 cut(s) 602, 711
PaeI GCATGC 1 cut(s) 768
PciSI GCTCTTC 1 cut(s) 337
PctI GAATGC 1 cut(s) 757
PdmI GAANNNNTTC 1 cut(s) 377
PfeI GAWTC 2 cut(s) 309, 604
PinAI ACCGGT 1 cut(s) 837
PkrI GCNGC 2 cut(s) 325, 770
PleI GAGTC 2 cut(s) 109, 462
PluTI GGCGCC 1 cut(s) 401
PpsI GAGTC 2 cut(s) 109, 462
PpuMI RGGWCCY 2 cut(s) 533, 639
PshAI GACNNNNGTC 1 cut(s) 644
Psp124BI GAGCTC 1 cut(s) 349
Psp5II RGGWCCY 2 cut(s) 533, 639
Psp6I CCWGG 1 cut(s) 287
PspGI CCWGG 1 cut(s) 287
PspN4I GGNNCC 2 cut(s) 399, 641
PspPI GGNCC 6 cut(s) 50, 167, 533, 639, 809, 835
PspPPI RGGWCCY 2 cut(s) 533, 639
PstI CTGCAG 1 cut(s) 773
PvuII CAGCTG 1 cut(s) 164
RsaI GTAC 1 cut(s) 494
RsaNI GTAC 1 cut(s) 493
SacI GAGCTC 1 cut(s) 349
SapI GCTCTTC 1 cut(s) 337
SaqAI TTAA 3 cut(s) 126, 189, 219
SatI GCNGC 2 cut(s) 324, 769
Sau3AI GATC 3 cut(s) 459, 577, 891
Sau96I GGNCC 6 cut(s) 50, 167, 533, 639, 809, 835
SchI GAGTC 2 cut(s) 109, 462
ScrFI CCNGG 1 cut(s) 289
SduI GDGCHC 1 cut(s) 349
SfaNI GCATC 3 cut(s) 250, 850, 868
SfcI CTRYAG 1 cut(s) 769
SfoI GGCGCC 1 cut(s) 399
SfuI TTCGAA 2 cut(s) 602, 711
SinI GGWCC 5 cut(s) 167, 533, 639, 809, 835
SmlI CTYRAG 1 cut(s) 304
SmoI CTYRAG 1 cut(s) 304
SphI GCATGC 1 cut(s) 768
Sse9I AATT 8 cut(s) 174, 213, 480, 496, 596, 679, 717, 798
SsiI CCGC 3 cut(s) 324, 593, 807
SspDI GGCGCC 1 cut(s) 397
SspI AATATT 1 cut(s) 237
SspMI CTAG 1 cut(s) 611
SstI GAGCTC 1 cut(s) 349
StyD4I CCNGG 1 cut(s) 287
TaaI ACNGT 2 cut(s) 492, 618
TaqI TCGA 4 cut(s) 371, 602, 711, 747
TaqII GACCGA 2 cut(s) 302, 431
TasI AATT 8 cut(s) 174, 213, 480, 496, 596, 679, 717, 798
TatI WGTACW 1 cut(s) 492
TauI GCSGC 1 cut(s) 326
TfiI GAWTC 2 cut(s) 309, 604
Tru1I TTAA 3 cut(s) 126, 189, 219
Tru9I TTAA 3 cut(s) 126, 189, 219
TscAI CASTG 1 cut(s) 931
TseFI GTSAC 1 cut(s) 646
TseI GCWGC 1 cut(s) 768
Tsp45I GTSAC 1 cut(s) 646
TspDTI ATGAA 3 cut(s) 237, 370, 956
TspRI CASTG 1 cut(s) 931
VpaK11BI GGWCC 5 cut(s) 167, 533, 639, 809, 835
XapI RAATTY 2 cut(s) 213, 596
XceI RCATGY 1 cut(s) 768
XmiI GTMKAC 2 cut(s) 31, 547
XmnI GAANNNNTTC 1 cut(s) 377
XspI CTAG 1 cut(s) 611
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.