Rorug04G0204600

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0001879
Physical Location & Seq
Reverse (-)
35700826 .. 35701337
512 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0204600.1

Sequence Viewer

Length: 297 bp
ATGCTTATGCAAATGCATTTACCTGTTTTCCTGAGATCAGTAAAGAGTTTTCAAGCTTTGTATGGGAATGCCAATATGACATGTACAAGAATTGTACCTACAGTCATCCACATCGAGCGGATCACCCGCGAGAAGGTCAACGTCGGAATCAACCCCTCCAAGGTCCTCATCACCAAGCTCAAGTTGGACAAGGACCGCAAGTCGCTGCTGGACCGCAAGGCCAAGGGAAGAGCCGCCGCCGATAAGGACAAAGGCACCAAGTTCACGGCGGAGGACATCATGCAGAACGTGGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

98

Amino Acids

11.11

Weight (kDa)

10.07

Isoelectric Point (pI)

25.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_L26 PF16906 35 - 73 2.3e-10 Ribosomal proteins L26 eukaryotic, L24P archaeal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 254
AccBSI CCGCTC 1 cut(s) 118
AccII CGCG 1 cut(s) 129
AciI CCGC 7 cut(s) 118, 127, 196, 214, 234, 237, 269
AclWI GGATC 1 cut(s) 128
AfaI GTAC 2 cut(s) 85, 96
AfiI CCNNNNNNNGG 2 cut(s) 133, 160
AflIII ACRYGT 1 cut(s) 80
AgsI TTSAA 1 cut(s) 53
AhdI GACNNNNNGTC 1 cut(s) 199
AluBI AGCT 2 cut(s) 56, 178
AluI AGCT 2 cut(s) 56, 178
AlwI GGATC 1 cut(s) 128
AoxI GGCC 1 cut(s) 219
ApeKI GCWGC 1 cut(s) 205
AspS9I GGNCC 3 cut(s) 163, 193, 211
AsuHPI GGTGA 2 cut(s) 115, 163
AvaII GGWCC 3 cut(s) 163, 193, 211
BanI GGYRCC 1 cut(s) 254
BbvI GCAGC 1 cut(s) 192
BceAI ACGGC 1 cut(s) 282
BfmI CTRYAG 1 cut(s) 99
BisI GCNGC 3 cut(s) 206, 234, 237
BlsI GCNGC 3 cut(s) 207, 235, 238
Bme18I GGWCC 3 cut(s) 163, 193, 211
BmeRI GACNNNNNGTC 1 cut(s) 199
BmgT120I GGNCC 3 cut(s) 163, 193, 211
BmiI GGNNCC 1 cut(s) 256
BpuEI CTTGAG 1 cut(s) 164
BsaJI CCNNGG 2 cut(s) 159, 222
Bsc4I CCNNNNNNNGG 2 cut(s) 133, 160
BseDI CCNNGG 2 cut(s) 159, 222
BseGI GGATG 1 cut(s) 105
BseLI CCNNNNNNNGG 2 cut(s) 133, 160
BseMII CTCAG 1 cut(s) 23
BseXI GCAGC 1 cut(s) 192
Bsh1236I CGCG 1 cut(s) 129
BshFI GGCC 1 cut(s) 221
BshNI GGYRCC 1 cut(s) 254
BslI CCNNNNNNNGG 2 cut(s) 133, 160
BsmI GAATGC 1 cut(s) 73
BsnI GGCC 1 cut(s) 221
Bsp1407I TGTACA 1 cut(s) 83
Bsp143I GATC 2 cut(s) 35, 120
BspACI CCGC 7 cut(s) 118, 127, 196, 214, 234, 237, 269
BspANI GGCC 1 cut(s) 221
BspCNI CTCAG 1 cut(s) 24
BspFNI CGCG 1 cut(s) 129
BspLI GGNNCC 1 cut(s) 256
BspPI GGATC 1 cut(s) 128
BspQI GCTCTTC 1 cut(s) 223
BspT107I GGYRCC 1 cut(s) 254
BsrBI CCGCTC 1 cut(s) 118
BsrGI TGTACA 1 cut(s) 83
BssECI CCNNGG 2 cut(s) 159, 222
BssMI GATC 2 cut(s) 35, 120
BssT1I CCWWGG 2 cut(s) 159, 222
Bst4CI ACNGT 1 cut(s) 103
Bst6I CTCTTC 1 cut(s) 223
BstAUI TGTACA 1 cut(s) 83
BstDEI CTNAG 1 cut(s) 32
BstF5I GGATG 1 cut(s) 105
BstFNI CGCG 1 cut(s) 129
BstKTI GATC 2 cut(s) 38, 123
BstMBI GATC 2 cut(s) 35, 120
BstNSI RCATGY 1 cut(s) 84
BstSFI CTRYAG 1 cut(s) 99
BstUI CGCG 1 cut(s) 129
BstV1I GCAGC 1 cut(s) 192
BsuRI GGCC 1 cut(s) 221
BtsCI GGATG 1 cut(s) 105
Cfr13I GGNCC 3 cut(s) 163, 193, 211
Csp6I GTAC 2 cut(s) 84, 95
CviAII CATG 2 cut(s) 81, 280
CviJI RGCY 4 cut(s) 56, 178, 221, 233
CviKI_1 RGCY 4 cut(s) 56, 178, 221, 233
CviQI GTAC 2 cut(s) 84, 95
DdeI CTNAG 1 cut(s) 32
DpnI GATC 2 cut(s) 37, 122
DpnII GATC 2 cut(s) 35, 120
DriI GACNNNNNGTC 1 cut(s) 199
Eam1104I CTCTTC 1 cut(s) 223
Eam1105I GACNNNNNGTC 1 cut(s) 199
EarI CTCTTC 1 cut(s) 223
EciI GGCGGA 1 cut(s) 284
Eco130I CCWWGG 2 cut(s) 159, 222
Eco47I GGWCC 3 cut(s) 163, 193, 211
EcoO109I RGGNCCY 1 cut(s) 163
EcoT14I CCWWGG 2 cut(s) 159, 222
EcoT22I ATGCAT 1 cut(s) 18
ErhI CCWWGG 2 cut(s) 159, 222
FaeI CATG 2 cut(s) 84, 283
FaiI YATR 5 cut(s) 8, 63, 77, 82, 281
FatI CATG 2 cut(s) 80, 279
FauI CCCGC 1 cut(s) 134
Fnu4HI GCNGC 3 cut(s) 206, 234, 237
FokI GGATG 1 cut(s) 92
Fsp4HI GCNGC 3 cut(s) 206, 234, 237
GluI GCNGC 3 cut(s) 206, 234, 237
HaeIII GGCC 1 cut(s) 221
Hin1II CATG 2 cut(s) 84, 283
HincII GTYRAC 1 cut(s) 139
HindII GTYRAC 1 cut(s) 139
HindIII AAGCTT 1 cut(s) 54
HinfI GANTC 1 cut(s) 147
HphI GGTGA 2 cut(s) 115, 163
Hpy166II GTNNAC 2 cut(s) 139, 264
Hpy188I TCNGA 1 cut(s) 146
Hpy188III TCNNGA 1 cut(s) 31
Hpy8I GTNNAC 2 cut(s) 139, 264
Hpy99I CGWCG 1 cut(s) 146
HpyAV CCTTC 1 cut(s) 127
HpyCH4III ACNGT 1 cut(s) 103
HpyCH4IV ACGT 2 cut(s) 141, 288
HpyCH4V TGCA 3 cut(s) 10, 16, 283
HpyF3I CTNAG 1 cut(s) 32
HpySE526I ACGT 2 cut(s) 141, 288
Hsp92II CATG 2 cut(s) 84, 283
Kzo9I GATC 2 cut(s) 35, 120
LguI GCTCTTC 1 cut(s) 223
LpnPI CCDG 3 cut(s) 36, 44, 194
Lsp1109I GCAGC 1 cut(s) 192
MaeII ACGT 2 cut(s) 141, 288
MalI GATC 2 cut(s) 37, 122
MbiI CCGCTC 1 cut(s) 118
MboI GATC 2 cut(s) 35, 120
MboII GAAGA 1 cut(s) 240
MluCI AATT 1 cut(s) 90
MmeI TCCRAC 2 cut(s) 124, 165
MnlI CCTC 3 cut(s) 166, 176, 265
Mph1103I ATGCAT 1 cut(s) 18
Mva1269I GAATGC 1 cut(s) 73
MvnI CGCG 1 cut(s) 129
NdeII GATC 2 cut(s) 35, 120
NlaIII CATG 2 cut(s) 84, 283
NlaIV GGNNCC 1 cut(s) 256
NsiI ATGCAT 1 cut(s) 18
NspI RCATGY 1 cut(s) 84
PciI ACATGT 1 cut(s) 80
PciSI GCTCTTC 1 cut(s) 223
PctI GAATGC 1 cut(s) 73
PfeI GAWTC 1 cut(s) 147
PkrI GCNGC 3 cut(s) 207, 235, 238
PpuMI RGGWCCY 1 cut(s) 163
PscI ACATGT 1 cut(s) 80
Psp5II RGGWCCY 1 cut(s) 163
PspN4I GGNNCC 1 cut(s) 256
PspPI GGNCC 3 cut(s) 163, 193, 211
PspPPI RGGWCCY 1 cut(s) 163
RsaI GTAC 2 cut(s) 85, 96
RsaNI GTAC 2 cut(s) 84, 95
SapI GCTCTTC 1 cut(s) 223
SatI GCNGC 3 cut(s) 206, 234, 237
Sau3AI GATC 2 cut(s) 35, 120
Sau96I GGNCC 3 cut(s) 163, 193, 211
SetI ASST 8 cut(s) 25, 58, 100, 138, 144, 165, 180, 291
SfcI CTRYAG 1 cut(s) 99
SinI GGWCC 3 cut(s) 163, 193, 211
SmlI CTYRAG 1 cut(s) 179
SmoI CTYRAG 1 cut(s) 179
Sse9I AATT 1 cut(s) 90
SsiI CCGC 7 cut(s) 118, 127, 196, 214, 234, 237, 269
StyI CCWWGG 2 cut(s) 159, 222
TaaI ACNGT 1 cut(s) 103
TaiI ACGT 2 cut(s) 144, 291
TaqI TCGA 1 cut(s) 114
TasI AATT 1 cut(s) 90
TatI WGTACW 1 cut(s) 83
TauI GCSGC 2 cut(s) 236, 239
TfiI GAWTC 1 cut(s) 147
TseI GCWGC 1 cut(s) 205
VpaK11BI GGWCC 3 cut(s) 163, 193, 211
XceI RCATGY 1 cut(s) 84
XcmI CCANNNNNNNNNTGG 1 cut(s) 181
Zsp2I ATGCAT 1 cut(s) 18
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.