Rroxscaffold_5G00367580

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0001879
Physical Location & Seq
Forward (+)
49401393 .. 49403530
2138 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00367580.1

Sequence Viewer

Length: 1038 bp
ATGTGTTCAGCAAAAGAAACTCCTGCTAATTTTGTGTTTGGCGATTCTCTAGTTGAAGTGGGGAACAACAACTATATTGTGACTCTCTCCAAGGCTGATTATCCTCCCAATGGAATCGATTTCGGAAAGCCTACCGGGAGATACACAAATGGAAGAACCATCATTGACATTATAGGTCAGACTCTTGGTTTTAAGGGGTTCACTCCTCCTTACTTAGCTCCAACAACAGCTGGACCAGTAATTCTGAGGGGTGTTAACTATGCTTCAGGTGGAGGTGGAATTCTTAATGAAACAGGAAAAATATTTGTTGGTAGAATAAACTTGGATGCACAGATAGATAACTTTGCAAATACCAGGCAAGACATTATCTCAAGAATCGGTCTTCCTGCAGCTCTAAAGTTACTTAGAAGAGCTCTGTTCTCAGTAACAATCGGCTCGAATGATTTCATCAATAACTACTTGGCGCCAGTTATATCTGAGACCGAGAGGAAGTCGATAACTCCGGAAACCTTTGTTGGCATTTTGATCGCAAGACTCAGACTACAAATTACAAGACTGTACAATTTGGGTGCAAGGAAGATGGTTGTGGTAAATGTAGGACCTATTGGGTGTATACCATATCAAAGGGAAGTAAATCAAGCTGATCCAGAGAGCTGTGTGGAATTTTCGAATCAACTAGCACGGTCATTCAATGCCAAGCTAAGGACCCTTGTCACCGAGCTAAACAAGAACCTTCCTACCGCCAATTTTGTTTATGCAGATGTTTATAGTATTGTGGAAGACATTATTGAGAACTACATATCATATGGTTTCGAGAATGCAAACTCTGCATGCTGCAGAGTAGCAGGGCGCTACGGGGGTCTAATTCCATGCGGTCCTCAGCCATCAAAAGTTTGCGTGGACCGGTCGAAGTATGTGTTTTGGGATGCTTACCATCCTTCTGATGCTTCTAATATGATCATAGCAAGGCGTCTCTTGTATGGGAGCTCCAGTGATATTTCACCCATGAATGTAGTGCAACTTCTCCAAGCTTCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

345

Amino Acids

37.7

Weight (kDa)

8.75

Isoelectric Point (pI)

31.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 11 - 324 3.8e-33 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 463
AccI GTMKAC 1 cut(s) 613
AccIII TCCGGA 1 cut(s) 502
AciI CCGC 2 cut(s) 741, 873
AclWI GGATC 1 cut(s) 638
AcsI RAATTY 2 cut(s) 279, 662
AcuI CTGAAG 1 cut(s) 249
AcyI GRCGYC 2 cut(s) 464, 970
AfaI GTAC 1 cut(s) 560
AfiI CCNNNNNNNGG 2 cut(s) 110, 702
AgeI ACCGGT 1 cut(s) 903
AgsI TTSAA 2 cut(s) 56, 691
AjnI CCWGG 1 cut(s) 353
AjuI GAANNNNNNNTTGG 2 cut(s) 498, 530
Alw21I GWGCWC 2 cut(s) 415, 989
Alw26I GTCTC 2 cut(s) 473, 977
AlwI GGATC 1 cut(s) 638
Aor13HI TCCGGA 1 cut(s) 502
ApeKI GCWGC 2 cut(s) 389, 834
ApoI RAATTY 2 cut(s) 279, 662
AsiGI ACCGGT 1 cut(s) 903
Asp700I GAANNNNTTC 1 cut(s) 443
AspLEI GCGC 2 cut(s) 466, 852
AspS9I GGNCC 5 cut(s) 233, 599, 705, 875, 901
AsuC2I CCSGG 1 cut(s) 136
AsuHPI GGTGA 2 cut(s) 706, 993
AsuII TTCGAA 1 cut(s) 668
AvaII GGWCC 5 cut(s) 233, 599, 705, 875, 901
BanI GGYRCC 1 cut(s) 463
BanII GRGCYC 2 cut(s) 415, 989
BarI GAAGNNNNNNTAC 2 cut(s) 1005, 1037
BbsI GAAGAC 2 cut(s) 374, 786
Bbv12I GWGCWC 2 cut(s) 415, 989
BbvCI CCTCAGC 1 cut(s) 879
BbvI GCAGC 2 cut(s) 401, 821
BccI CCATC 4 cut(s) 167, 574, 892, 942
BcgI CGANNNNNNTGC 2 cut(s) 508, 542
BciT130I CCWGG 1 cut(s) 355
BclI TGATCA 1 cut(s) 957
BcnI CCSGG 1 cut(s) 136
BcoDI GTCTC 2 cut(s) 473, 977
BfaI CTAG 2 cut(s) 50, 677
BfmI CTRYAG 2 cut(s) 387, 835
BfoI RGCGCY 2 cut(s) 467, 853
BisI GCNGC 2 cut(s) 390, 835
BlsI GCNGC 2 cut(s) 391, 836
Bme1390I CCNGG 2 cut(s) 136, 355
Bme18I GGWCC 5 cut(s) 233, 599, 705, 875, 901
BmgT120I GGNCC 5 cut(s) 233, 599, 705, 875, 901
BmiI GGNNCC 2 cut(s) 465, 707
BmrFI CCNGG 2 cut(s) 136, 355
BmsI GCATC 3 cut(s) 316, 916, 934
BoxI GACNNNNGTC 1 cut(s) 710
BpiI GAAGAC 2 cut(s) 374, 786
BpmI CTGGAG 1 cut(s) 973
Bpu10I CCTNAGC 2 cut(s) 701, 879
Bpu14I TTCGAA 1 cut(s) 668
BpuEI CTTGAG 1 cut(s) 355
BpuMI CCSGG 1 cut(s) 136
Bsa29I ATCGAT 1 cut(s) 117
BsaHI GRCGYC 2 cut(s) 464, 970
BsaI GGTCTC 1 cut(s) 473
BsaJI CCNNGG 1 cut(s) 90
BsaWI WCCGGW 2 cut(s) 502, 903
BsaXI ACNNNNNCTCC 2 cut(s) 976, 1006
Bsc4I CCNNNNNNNGG 2 cut(s) 110, 702
Bse118I RCCGGY 1 cut(s) 903
Bse1I ACTGG 3 cut(s) 236, 467, 990
BseAI TCCGGA 1 cut(s) 502
BseBI CCWGG 1 cut(s) 355
BseCI ATCGAT 1 cut(s) 117
BseDI CCNNGG 1 cut(s) 90
BseGI GGATG 3 cut(s) 331, 931, 934
BseLI CCNNNNNNNGG 2 cut(s) 110, 702
BseMII CTCAG 5 cut(s) 236, 435, 468, 550, 893
BseNI ACTGG 3 cut(s) 236, 467, 990
BseRI GAGGAG 1 cut(s) 195
BseXI GCAGC 2 cut(s) 401, 821
Bsh1285I CGRYCG 1 cut(s) 908
BshNI GGYRCC 1 cut(s) 463
BshTI ACCGGT 1 cut(s) 903
BshVI ATCGAT 1 cut(s) 117
BsiEI CGRYCG 1 cut(s) 908
BsiHKAI GWGCWC 2 cut(s) 415, 989
BsiSI CCGG 3 cut(s) 135, 503, 904
BslI CCNNNNNNNGG 2 cut(s) 110, 702
BsmAI GTCTC 2 cut(s) 473, 977
BsmBI CGTCTC 1 cut(s) 977
BsmI GAATGC 1 cut(s) 823
Bso31I GGTCTC 1 cut(s) 473
Bsp119I TTCGAA 1 cut(s) 668
Bsp1286I GDGCHC 2 cut(s) 415, 989
Bsp13I TCCGGA 1 cut(s) 502
Bsp1407I TGTACA 1 cut(s) 558
Bsp143I GATC 3 cut(s) 525, 643, 957
BspACI CCGC 2 cut(s) 741, 873
BspCNI CTCAG 5 cut(s) 237, 434, 469, 549, 892
BspDI ATCGAT 1 cut(s) 117
BspEI TCCGGA 1 cut(s) 502
BspLI GGNNCC 2 cut(s) 465, 707
BspMAI CTGCAG 2 cut(s) 391, 839
BspPI GGATC 1 cut(s) 638
BspQI GCTCTTC 1 cut(s) 403
BspT104I TTCGAA 1 cut(s) 668
BspT107I GGYRCC 1 cut(s) 463
BspTNI GGTCTC 1 cut(s) 473
BsrFI RCCGGY 1 cut(s) 903
BsrGI TGTACA 1 cut(s) 558
BsrI ACTGG 3 cut(s) 236, 467, 990
BssAI RCCGGY 1 cut(s) 903
BssECI CCNNGG 1 cut(s) 90
BssMI GATC 3 cut(s) 525, 643, 957
BssNAI GTATAC 1 cut(s) 614
BssNI GRCGYC 2 cut(s) 464, 970
BssT1I CCWWGG 1 cut(s) 90
Bst1107I GTATAC 1 cut(s) 614
Bst2UI CCWGG 1 cut(s) 355
Bst4CI ACNGT 2 cut(s) 558, 684
Bst6I CTCTTC 1 cut(s) 403
BstACI GRCGYC 2 cut(s) 464, 970
BstAPI GCANNNNNTGC 1 cut(s) 827
BstAUI TGTACA 1 cut(s) 558
BstBI TTCGAA 1 cut(s) 668
BstC8I GCNNGC 1 cut(s) 832
BstDEI CTNAG 8 cut(s) 214, 245, 404, 421, 477, 536, 701, 879
BstF5I GGATG 3 cut(s) 331, 931, 934
BstH2I RGCGCY 2 cut(s) 467, 853
BstHHI GCGC 2 cut(s) 466, 852
BstKTI GATC 3 cut(s) 528, 646, 960
BstMAI GTCTC 2 cut(s) 473, 977
BstMBI GATC 3 cut(s) 525, 643, 957
BstMCI CGRYCG 1 cut(s) 908
BstMWI GCNNNNNNNGC 1 cut(s) 827
BstNI CCWGG 1 cut(s) 355
BstNSI RCATGY 1 cut(s) 834
BstPAI GACNNNNGTC 1 cut(s) 710
BstSCI CCNGG 2 cut(s) 134, 353
BstSFI CTRYAG 2 cut(s) 387, 835
BstV1I GCAGC 2 cut(s) 401, 821
BstV2I GAAGAC 2 cut(s) 374, 786
BstZ17I GTATAC 1 cut(s) 614
Bsu15I ATCGAT 1 cut(s) 117
BsuTUI ATCGAT 1 cut(s) 117
BtsCI GGATG 3 cut(s) 331, 931, 934
BtsIMutI CAGTG 1 cut(s) 997
Cac8I GCNNGC 1 cut(s) 832
CfoI GCGC 2 cut(s) 466, 852
Cfr10I RCCGGY 1 cut(s) 903
Cfr13I GGNCC 5 cut(s) 233, 599, 705, 875, 901
ClaI ATCGAT 1 cut(s) 117
CseI GACGC 1 cut(s) 959
Csp6I GTAC 1 cut(s) 559
CspAI ACCGGT 1 cut(s) 903
CviAII CATG 3 cut(s) 831, 870, 1006
CviQI GTAC 1 cut(s) 559
DdeI CTNAG 8 cut(s) 214, 245, 404, 421, 477, 536, 701, 879
DinI GGCGCC 1 cut(s) 465
DpnI GATC 3 cut(s) 527, 645, 959
DpnII GATC 3 cut(s) 525, 643, 957
Eam1104I CTCTTC 1 cut(s) 403
EarI CTCTTC 1 cut(s) 403
Ecl136II GAGCTC 2 cut(s) 413, 987
Eco130I CCWWGG 1 cut(s) 90
Eco24I GRGCYC 2 cut(s) 415, 989
Eco31I GGTCTC 1 cut(s) 473
Eco47I GGWCC 5 cut(s) 233, 599, 705, 875, 901
Eco53kI GAGCTC 2 cut(s) 413, 987
Eco57I CTGAAG 1 cut(s) 249
EcoICRI GAGCTC 2 cut(s) 413, 987
EcoO109I RGGNCCY 2 cut(s) 599, 705
EcoRI GAATTC 1 cut(s) 279
EcoRII CCWGG 1 cut(s) 353
EcoT14I CCWWGG 1 cut(s) 90
EcoT38I GRGCYC 2 cut(s) 415, 989
EgeI GGCGCC 1 cut(s) 465
EheI GGCGCC 1 cut(s) 465
ErhI CCWWGG 1 cut(s) 90
Esp3I CGTCTC 1 cut(s) 977
FaeI CATG 3 cut(s) 834, 873, 1009
FatI CATG 3 cut(s) 830, 869, 1005
FauNDI CATATG 1 cut(s) 805
FbaI TGATCA 1 cut(s) 957
FblI GTMKAC 1 cut(s) 613
Fnu4HI GCNGC 2 cut(s) 390, 835
FokI GGATG 3 cut(s) 338, 921, 938
FriOI GRGCYC 2 cut(s) 415, 989
Fsp4HI GCNGC 2 cut(s) 390, 835
FspBI CTAG 2 cut(s) 50, 677
GlaI GCGC 2 cut(s) 465, 851
GluI GCNGC 2 cut(s) 390, 835
GsuI CTGGAG 1 cut(s) 973
HaeII RGCGCY 2 cut(s) 467, 853
HapII CCGG 3 cut(s) 135, 503, 904
HgaI GACGC 1 cut(s) 959
HhaI GCGC 2 cut(s) 466, 852
Hin1I GRCGYC 2 cut(s) 464, 970
Hin1II CATG 3 cut(s) 834, 873, 1009
Hin6I GCGC 2 cut(s) 464, 850
HinP1I GCGC 2 cut(s) 464, 850
HincII GTYRAC 1 cut(s) 256
HindII GTYRAC 1 cut(s) 256
HindIII AAGCTT 1 cut(s) 1029
HinfI GANTC 7 cut(s) 44, 82, 114, 181, 375, 534, 670
HpaI GTTAAC 1 cut(s) 256
HpaII CCGG 3 cut(s) 135, 503, 904
HphI GGTGA 2 cut(s) 706, 993
Hpy166II GTNNAC 4 cut(s) 201, 256, 614, 901
Hpy188I TCNGA 6 cut(s) 125, 180, 246, 478, 539, 943
Hpy188III TCNNGA 5 cut(s) 372, 503, 647, 814, 1035
Hpy8I GTNNAC 4 cut(s) 201, 256, 614, 901
HpyAV CCTTC 2 cut(s) 743, 948
HpyCH4III ACNGT 2 cut(s) 558, 684
HpyCH4V TGCA 9 cut(s) 329, 347, 389, 572, 758, 821, 830, 837, 1018
HpyF10VI GCNNNNNNNGC 1 cut(s) 827
HpyF3I CTNAG 8 cut(s) 214, 245, 404, 421, 477, 536, 701, 879
Hsp92I GRCGYC 2 cut(s) 464, 970
Hsp92II CATG 3 cut(s) 834, 873, 1009
HspAI GCGC 2 cut(s) 464, 850
KasI GGCGCC 1 cut(s) 463
Kpn2I TCCGGA 1 cut(s) 502
Ksp22I TGATCA 1 cut(s) 957
KspAI GTTAAC 1 cut(s) 256
Kzo9I GATC 3 cut(s) 525, 643, 957
LguI GCTCTTC 1 cut(s) 403
LmnI GCTCC 3 cut(s) 223, 984, 992
Lsp1109I GCAGC 2 cut(s) 401, 821
LweI GCATC 3 cut(s) 316, 916, 934
MaeI CTAG 2 cut(s) 50, 677
MaeIII GTNAC 4 cut(s) 79, 399, 424, 712
MalI GATC 3 cut(s) 527, 645, 959
MboI GATC 3 cut(s) 525, 643, 957
MboII GAAGA 5 cut(s) 165, 374, 420, 589, 791
MhlI GDGCHC 2 cut(s) 415, 989
MluCI AATT 8 cut(s) 28, 240, 279, 546, 562, 662, 745, 864
Mly113I GGCGCC 1 cut(s) 464
MlyI GAGTC 3 cut(s) 76, 175, 528
MmeI TCCRAC 1 cut(s) 245
MnlI CCTC 6 cut(s) 114, 216, 240, 266, 480, 888
MroI TCCGGA 1 cut(s) 502
MroXI GAANNNNTTC 1 cut(s) 443
MseI TTAA 3 cut(s) 192, 255, 285
MspA1I CMGCKG 1 cut(s) 230
MspI CCGG 3 cut(s) 135, 503, 904
MspR9I CCNGG 2 cut(s) 136, 355
Mva1269I GAATGC 1 cut(s) 823
MvaI CCWGG 1 cut(s) 355
MwoI GCNNNNNNNGC 1 cut(s) 827
NarI GGCGCC 1 cut(s) 464
NciI CCSGG 1 cut(s) 136
NdeI CATATG 1 cut(s) 805
NdeII GATC 3 cut(s) 525, 643, 957
NlaIII CATG 3 cut(s) 834, 873, 1009
NlaIV GGNNCC 2 cut(s) 465, 707
NmuCI GTSAC 2 cut(s) 79, 712
NspI RCATGY 1 cut(s) 834
NspV TTCGAA 1 cut(s) 668
PaeI GCATGC 1 cut(s) 834
PciSI GCTCTTC 1 cut(s) 403
PctI GAATGC 1 cut(s) 823
PdmI GAANNNNTTC 1 cut(s) 443
PfeI GAWTC 4 cut(s) 44, 114, 375, 670
PinAI ACCGGT 1 cut(s) 903
PkrI GCNGC 2 cut(s) 391, 836
PleI GAGTC 3 cut(s) 76, 175, 528
PluTI GGCGCC 1 cut(s) 467
PpsI GAGTC 3 cut(s) 76, 175, 528
PpuMI RGGWCCY 2 cut(s) 599, 705
PshAI GACNNNNGTC 1 cut(s) 710
Psp124BI GAGCTC 2 cut(s) 415, 989
Psp5II RGGWCCY 2 cut(s) 599, 705
Psp6I CCWGG 1 cut(s) 353
PspGI CCWGG 1 cut(s) 353
PspN4I GGNNCC 2 cut(s) 465, 707
PspPI GGNCC 5 cut(s) 233, 599, 705, 875, 901
PspPPI RGGWCCY 2 cut(s) 599, 705
PstI CTGCAG 2 cut(s) 391, 839
PvuII CAGCTG 1 cut(s) 230
RsaI GTAC 1 cut(s) 560
RsaNI GTAC 1 cut(s) 559
SacI GAGCTC 2 cut(s) 415, 989
SapI GCTCTTC 1 cut(s) 403
SaqAI TTAA 3 cut(s) 192, 255, 285
SatI GCNGC 2 cut(s) 390, 835
Sau3AI GATC 3 cut(s) 525, 643, 957
Sau96I GGNCC 5 cut(s) 233, 599, 705, 875, 901
SchI GAGTC 3 cut(s) 76, 175, 528
ScrFI CCNGG 2 cut(s) 136, 355
SduI GDGCHC 2 cut(s) 415, 989
SfaNI GCATC 3 cut(s) 316, 916, 934
SfcI CTRYAG 2 cut(s) 387, 835
SfoI GGCGCC 1 cut(s) 465
SfuI TTCGAA 1 cut(s) 668
SinI GGWCC 5 cut(s) 233, 599, 705, 875, 901
SmlI CTYRAG 1 cut(s) 370
SmoI CTYRAG 1 cut(s) 370
SphI GCATGC 1 cut(s) 834
Sse9I AATT 8 cut(s) 28, 240, 279, 546, 562, 662, 745, 864
SsiI CCGC 2 cut(s) 741, 873
SspDI GGCGCC 1 cut(s) 463
SspI AATATT 1 cut(s) 303
SspMI CTAG 2 cut(s) 50, 677
SstI GAGCTC 2 cut(s) 415, 989
StyD4I CCNGG 2 cut(s) 134, 353
StyI CCWWGG 1 cut(s) 90
TaaI ACNGT 2 cut(s) 558, 684
TaqI TCGA 6 cut(s) 117, 437, 494, 668, 813, 908
TaqII GACCGA 2 cut(s) 368, 497
TasI AATT 8 cut(s) 28, 240, 279, 546, 562, 662, 745, 864
TatI WGTACW 1 cut(s) 558
TfiI GAWTC 4 cut(s) 44, 114, 375, 670
Tru1I TTAA 3 cut(s) 192, 255, 285
Tru9I TTAA 3 cut(s) 192, 255, 285
TscAI CASTG 1 cut(s) 997
TseFI GTSAC 2 cut(s) 79, 712
TseI GCWGC 2 cut(s) 389, 834
Tsp45I GTSAC 2 cut(s) 79, 712
TspDTI ATGAA 3 cut(s) 303, 436, 1022
TspRI CASTG 1 cut(s) 997
VpaK11BI GGWCC 5 cut(s) 233, 599, 705, 875, 901
XapI RAATTY 2 cut(s) 279, 662
XceI RCATGY 1 cut(s) 834
XmiI GTMKAC 1 cut(s) 613
XmnI GAANNNNTTC 1 cut(s) 443
XspI CTAG 2 cut(s) 50, 677
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.