FvH4_4g19872

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0001879
Physical Location & Seq
Forward (+)
23365785 .. 23369946
4162 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g19872.t1

Sequence Viewer

Length: 720 bp
ATGGATGCACAGATAGATTATTTTGCCAATACCAGACAAGACATTATCTCAAGCATTGGTCTTCCTGCAGCAGTAGAGCTACTTAGAAGATCTCTCTTCTCGGTAGAAATCGGCTCAAACGATTTCCTCAGTAACTACTTGGTGCCGGTTATAACTGAGGTTAAGAGGAAGTTGATACCTCCGGAAACCTTTGTTGGCATTTTGATTGCAAGATTCCGACTACAACTGACGAGACTGTATAAATTGGGTGCAAGGAAGATGGTTGTGATAAATATAGGACCTATTGGGTGCATACCATTTGAGAGGGAAGTAAATCTGGTAGCTGATCCAGATCTGTGTGCTGAATTTCCAAATCAATTAGCTCGATTATTCAACGCCAAGCTAAGGACCCTTGTCACCGAGCTAAACAAGAATCTTCCCGGGGCCAGATTTGTGTATGCAAATGCTTATAGTATTGTGGAAGACATAATTGACAACTATATATCATATGGTTTCGAGAATAGAAACTCTGCGTGCTGCAGTACAGTAGGGCGCTACGGGGGTCTAATCGCATGCGGTCCTCAACCCACAATGGTGTGCGAGGACCGATCGAAGTATGTGTTCTGGGATCCTTACCATCCTTCTGATGCTTCAAACATGATCATAGCAAGGCGTCTCTTGTATGGGAACTCCAGTGATATTTCACCCATGAATGTAGTGCAACTCCTCCAAGCTTCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

240

Amino Acids

26.77

Weight (kDa)

7.54

Isoelectric Point (pI)

46.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 29 - 218 7.6e-21 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 152
AccB1I GGYRCC 1 cut(s) 142
AccIII TCCGGA 1 cut(s) 181
AciI CCGC 1 cut(s) 555
AclWI GGATC 3 cut(s) 320, 602, 615
AcsI RAATTY 1 cut(s) 344
AcyI GRCGYC 1 cut(s) 652
AfaI GTAC 1 cut(s) 523
AfiI CCNNNNNNNGG 1 cut(s) 384
AgsI TTSAA 2 cut(s) 373, 633
AjuI GAANNNNNNNTTGG 2 cut(s) 177, 209
AleI CACNNNNGTG 1 cut(s) 572
AluBI AGCT 6 cut(s) 79, 323, 362, 382, 403, 713
AluI AGCT 6 cut(s) 79, 323, 362, 382, 403, 713
Alw26I GTCTC 2 cut(s) 226, 659
AlwI GGATC 3 cut(s) 320, 602, 615
Ama87I CYCGRG 1 cut(s) 419
Aor13HI TCCGGA 1 cut(s) 181
AoxI GGCC 1 cut(s) 423
ApeKI GCWGC 2 cut(s) 68, 516
ApoI RAATTY 1 cut(s) 344
AspLEI GCGC 1 cut(s) 534
AspS9I GGNCC 5 cut(s) 278, 387, 423, 557, 583
AsuC2I CCSGG 2 cut(s) 420, 421
AsuHPI GGTGA 2 cut(s) 388, 675
AvaI CYCGRG 1 cut(s) 419
AvaII GGWCC 4 cut(s) 278, 387, 557, 583
BamHI GGATCC 1 cut(s) 607
BanI GGYRCC 1 cut(s) 142
BbsI GAAGAC 2 cut(s) 53, 468
BbvI GCAGC 2 cut(s) 80, 503
BccI CCATC 2 cut(s) 253, 624
BclI TGATCA 1 cut(s) 639
BcnI CCSGG 2 cut(s) 420, 421
BcoDI GTCTC 2 cut(s) 226, 659
BfmI CTRYAG 2 cut(s) 66, 517
BfoI RGCGCY 1 cut(s) 535
BglII AGATCT 2 cut(s) 89, 331
BisI GCNGC 2 cut(s) 69, 517
BlsI GCNGC 2 cut(s) 70, 518
Bme1390I CCNGG 2 cut(s) 420, 421
Bme18I GGWCC 4 cut(s) 278, 387, 557, 583
BmeT110I CYCGRG 1 cut(s) 419
BmgT120I GGNCC 5 cut(s) 278, 387, 423, 557, 583
BmiI GGNNCC 4 cut(s) 144, 389, 424, 609
BmrFI CCNGG 2 cut(s) 420, 421
BmsI GCATC 1 cut(s) 616
BoxI GACNNNNGTC 1 cut(s) 392
BpiI GAAGAC 2 cut(s) 53, 468
BpmI CTGGAG 1 cut(s) 655
Bpu10I CCTNAGC 1 cut(s) 383
BpuEI CTTGAG 1 cut(s) 34
BpuMI CCSGG 2 cut(s) 420, 421
BsaBI GATNNNNATC 1 cut(s) 330
BsaHI GRCGYC 1 cut(s) 652
BsaJI CCNNGG 2 cut(s) 419, 420
BsaWI WCCGGW 1 cut(s) 181
Bsc4I CCNNNNNNNGG 1 cut(s) 384
Bse118I RCCGGY 1 cut(s) 145
Bse1I ACTGG 1 cut(s) 672
Bse8I GATNNNNATC 1 cut(s) 330
BseAI TCCGGA 1 cut(s) 181
BseDI CCNNGG 2 cut(s) 419, 420
BseGI GGATG 2 cut(s) 10, 616
BseJI GATNNNNATC 1 cut(s) 330
BseLI CCNNNNNNNGG 1 cut(s) 384
BseMII CTCAG 2 cut(s) 142, 147
BseNI ACTGG 1 cut(s) 672
BseRI GAGGAG 1 cut(s) 695
BseXI GCAGC 2 cut(s) 80, 503
Bsh1285I CGRYCG 1 cut(s) 590
BshFI GGCC 1 cut(s) 425
BshNI GGYRCC 1 cut(s) 142
BsiEI CGRYCG 1 cut(s) 590
BsiHKCI CYCGRG 1 cut(s) 419
BsiSI CCGG 3 cut(s) 146, 182, 420
BslI CCNNNNNNNGG 1 cut(s) 384
BsmAI GTCTC 2 cut(s) 226, 659
BsmBI CGTCTC 1 cut(s) 659
BsnI GGCC 1 cut(s) 425
BsoBI CYCGRG 1 cut(s) 419
Bsp13I TCCGGA 1 cut(s) 181
Bsp143I GATC 6 cut(s) 89, 325, 331, 587, 607, 639
BspACI CCGC 1 cut(s) 555
BspANI GGCC 1 cut(s) 425
BspCNI CTCAG 2 cut(s) 141, 148
BspEI TCCGGA 1 cut(s) 181
BspLI GGNNCC 4 cut(s) 144, 389, 424, 609
BspMAI CTGCAG 2 cut(s) 70, 521
BspPI GGATC 3 cut(s) 320, 602, 615
BspT107I GGYRCC 1 cut(s) 142
BsrFI RCCGGY 1 cut(s) 145
BsrI ACTGG 1 cut(s) 672
BssAI RCCGGY 1 cut(s) 145
BssECI CCNNGG 2 cut(s) 419, 420
BssMI GATC 6 cut(s) 89, 325, 331, 587, 607, 639
BssNI GRCGYC 1 cut(s) 652
Bst4CI ACNGT 2 cut(s) 237, 526
Bst6I CTCTTC 1 cut(s) 101
BstACI GRCGYC 1 cut(s) 652
BstC8I GCNNGC 2 cut(s) 514, 553
BstDEI CTNAG 4 cut(s) 83, 128, 156, 383
BstF5I GGATG 2 cut(s) 10, 616
BstH2I RGCGCY 1 cut(s) 535
BstHHI GCGC 1 cut(s) 534
BstKTI GATC 6 cut(s) 92, 328, 334, 590, 610, 642
BstMAI GTCTC 2 cut(s) 226, 659
BstMBI GATC 6 cut(s) 89, 325, 331, 587, 607, 639
BstMCI CGRYCG 1 cut(s) 590
BstNSI RCATGY 1 cut(s) 555
BstPAI GACNNNNGTC 1 cut(s) 392
BstSCI CCNGG 2 cut(s) 418, 419
BstSFI CTRYAG 2 cut(s) 66, 517
BstV1I GCAGC 2 cut(s) 80, 503
BstV2I GAAGAC 2 cut(s) 53, 468
BstX2I RGATCY 3 cut(s) 89, 331, 607
BstYI RGATCY 3 cut(s) 89, 331, 607
BsuRI GGCC 1 cut(s) 425
BtsCI GGATG 2 cut(s) 10, 616
BtsIMutI CAGTG 1 cut(s) 679
Cac8I GCNNGC 2 cut(s) 514, 553
CfoI GCGC 1 cut(s) 534
Cfr10I RCCGGY 1 cut(s) 145
Cfr13I GGNCC 5 cut(s) 278, 387, 423, 557, 583
Cfr9I CCCGGG 1 cut(s) 419
CseI GACGC 1 cut(s) 641
Csp6I GTAC 1 cut(s) 522
CviAII CATG 3 cut(s) 552, 637, 688
CviJI RGCY 8 cut(s) 79, 114, 323, 362, 382, 403, 425, 713
CviKI_1 RGCY 8 cut(s) 79, 114, 323, 362, 382, 403, 425, 713
CviQI GTAC 1 cut(s) 522
DdeI CTNAG 4 cut(s) 83, 128, 156, 383
DpnI GATC 6 cut(s) 91, 327, 333, 589, 609, 641
DpnII GATC 6 cut(s) 89, 325, 331, 587, 607, 639
Eam1104I CTCTTC 1 cut(s) 101
EarI CTCTTC 1 cut(s) 101
Eco47I GGWCC 4 cut(s) 278, 387, 557, 583
Eco88I CYCGRG 1 cut(s) 419
EcoO109I RGGNCCY 2 cut(s) 278, 387
Esp3I CGTCTC 1 cut(s) 659
FaeI CATG 3 cut(s) 555, 640, 691
FatI CATG 3 cut(s) 551, 636, 687
FauNDI CATATG 1 cut(s) 487
FbaI TGATCA 1 cut(s) 639
Fnu4HI GCNGC 2 cut(s) 69, 517
FokI GGATG 2 cut(s) 17, 603
Fsp4HI GCNGC 2 cut(s) 69, 517
GlaI GCGC 1 cut(s) 533
GluI GCNGC 2 cut(s) 69, 517
GsuI CTGGAG 1 cut(s) 655
HaeII RGCGCY 1 cut(s) 535
HaeIII GGCC 1 cut(s) 425
HapII CCGG 3 cut(s) 146, 182, 420
HgaI GACGC 1 cut(s) 641
HhaI GCGC 1 cut(s) 534
Hin1I GRCGYC 1 cut(s) 652
Hin1II CATG 3 cut(s) 555, 640, 691
Hin6I GCGC 1 cut(s) 532
HinP1I GCGC 1 cut(s) 532
HindIII AAGCTT 1 cut(s) 711
HinfI GANTC 2 cut(s) 213, 412
HpaII CCGG 3 cut(s) 146, 182, 420
HphI GGTGA 2 cut(s) 388, 675
Hpy188I TCNGA 2 cut(s) 218, 625
Hpy188III TCNNGA 4 cut(s) 182, 329, 496, 717
HpyAV CCTTC 1 cut(s) 630
HpyCH4III ACNGT 2 cut(s) 237, 526
HpyCH4V TGCA 8 cut(s) 8, 68, 209, 251, 291, 440, 519, 700
HpyF3I CTNAG 4 cut(s) 83, 128, 156, 383
Hsp92I GRCGYC 1 cut(s) 652
Hsp92II CATG 3 cut(s) 555, 640, 691
HspAI GCGC 1 cut(s) 532
Kpn2I TCCGGA 1 cut(s) 181
Ksp22I TGATCA 1 cut(s) 639
Kzo9I GATC 6 cut(s) 89, 325, 331, 587, 607, 639
Lsp1109I GCAGC 2 cut(s) 80, 503
LweI GCATC 1 cut(s) 616
MaeIII GTNAC 2 cut(s) 131, 394
MalI GATC 6 cut(s) 91, 327, 333, 589, 609, 641
MboI GATC 6 cut(s) 89, 325, 331, 587, 607, 639
MboII GAAGA 6 cut(s) 53, 88, 99, 268, 407, 473
MflI RGATCY 3 cut(s) 89, 331, 607
MluCI AATT 4 cut(s) 242, 344, 356, 468
MmeI TCCRAC 1 cut(s) 241
MnlI CCTC 8 cut(s) 137, 151, 159, 189, 297, 570, 574, 716
MroI TCCGGA 1 cut(s) 181
MseI TTAA 1 cut(s) 162
MslI CAYNNNNRTG 1 cut(s) 572
MspI CCGG 3 cut(s) 146, 182, 420
MspR9I CCNGG 2 cut(s) 420, 421
NciI CCSGG 2 cut(s) 420, 421
NdeI CATATG 1 cut(s) 487
NdeII GATC 6 cut(s) 89, 325, 331, 587, 607, 639
NlaIII CATG 3 cut(s) 555, 640, 691
NlaIV GGNNCC 4 cut(s) 144, 389, 424, 609
NmuCI GTSAC 1 cut(s) 394
NspI RCATGY 1 cut(s) 555
OliI CACNNNNGTG 1 cut(s) 572
PaeI GCATGC 1 cut(s) 555
PcsI WCGNNNNNNNCGW 1 cut(s) 117
PfeI GAWTC 2 cut(s) 213, 412
PkrI GCNGC 2 cut(s) 70, 518
Ple19I CGATCG 1 cut(s) 590
PpuMI RGGWCCY 2 cut(s) 278, 387
PshAI GACNNNNGTC 1 cut(s) 392
PsiI TTATAA 1 cut(s) 152
Psp5II RGGWCCY 2 cut(s) 278, 387
PspN4I GGNNCC 4 cut(s) 144, 389, 424, 609
PspPI GGNCC 5 cut(s) 278, 387, 423, 557, 583
PspPPI RGGWCCY 2 cut(s) 278, 387
PstI CTGCAG 2 cut(s) 70, 521
PsuI RGATCY 3 cut(s) 89, 331, 607
PvuI CGATCG 1 cut(s) 590
RsaI GTAC 1 cut(s) 523
RsaNI GTAC 1 cut(s) 522
RseI CAYNNNNRTG 1 cut(s) 572
SaqAI TTAA 1 cut(s) 162
SatI GCNGC 2 cut(s) 69, 517
Sau3AI GATC 6 cut(s) 89, 325, 331, 587, 607, 639
Sau96I GGNCC 5 cut(s) 278, 387, 423, 557, 583
ScrFI CCNGG 2 cut(s) 420, 421
SfaNI GCATC 1 cut(s) 616
SfcI CTRYAG 2 cut(s) 66, 517
SinI GGWCC 4 cut(s) 278, 387, 557, 583
SmaI CCCGGG 1 cut(s) 421
SmiMI CAYNNNNRTG 1 cut(s) 572
SmlI CTYRAG 1 cut(s) 49
SmoI CTYRAG 1 cut(s) 49
SphI GCATGC 1 cut(s) 555
Sse9I AATT 4 cut(s) 242, 344, 356, 468
SsiI CCGC 1 cut(s) 555
StyD4I CCNGG 2 cut(s) 418, 419
TaaI ACNGT 2 cut(s) 237, 526
TaqI TCGA 3 cut(s) 364, 495, 590
TaqII GACCGA 1 cut(s) 600
TasI AATT 4 cut(s) 242, 344, 356, 468
TatI WGTACW 1 cut(s) 521
TfiI GAWTC 2 cut(s) 213, 412
Tru1I TTAA 1 cut(s) 162
Tru9I TTAA 1 cut(s) 162
TscAI CASTG 1 cut(s) 679
TseFI GTSAC 1 cut(s) 394
TseI GCWGC 2 cut(s) 68, 516
Tsp45I GTSAC 1 cut(s) 394
TspDTI ATGAA 1 cut(s) 704
TspMI CCCGGG 1 cut(s) 419
TspRI CASTG 1 cut(s) 679
VpaK11BI GGWCC 4 cut(s) 278, 387, 557, 583
XapI RAATTY 1 cut(s) 344
XceI RCATGY 1 cut(s) 555
XmaI CCCGGG 1 cut(s) 419
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.