Rroxscaffold_5G00367420

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0001879
Physical Location & Seq
Forward (+)
49269092 .. 49272190
3099 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00367420.1

Sequence Viewer

Length: 1281 bp
ATGGTTGCAAGAGTGGAAGGCAAAGATGAGATCCGGCCGAATGAGAATTGGCGTGCGAAGCTCGATCTTGACCGCGAGGGCGAGATGTTCGATTTGCAACACGATGTTCGACAGATTCGGCGAGAAGATAGAGCTTGGGTGTCCCGAAAATTTGAGATACCCTTTTGCAAAACCTTTATAATGGGGTACCTGTTTTCAGAAGGAAAATTCGTTCTTGGCATTATTTTTGCACTTTGGAGAATGTGTTCAGCAAAAGAAATTCCTACTAATTTTGTGTTTGGCGATTCTCTAGTTGAAGTGGGGAACAACAACTATATTGTGACTCTCTCCAAGGCTGATCATCCTCCCTATGGAATTGATTTCGGAAAATCTACCGGGAGATACACAAATGGAAGAACCATCATTGATATTGCGGGTCAGACTCTGGGTTTAAGGGAGTTCACTCCTCCTTACTTGGCTCCCACAACAGCTGGACCAGTAATTCTGAGGGGTGTCAACTATGCTTCAGGTGGAGGTGGAATTCTTAACGAAACAGGAAAATTATTTATTGGTAGAATAAACTTGGATGCACAGATAGATAACTTTGCAAATACCAGAGAAGACATTATCTCAAGCATTGGTCGTCCTGCAGCAGTAGAGCTACTTAGAAGAGCTCTCTTCTCGGTAACAATCGGGTCCAACGATTTCCTCAATAACTACTTGTTGCCAGTTATAACTGAGTTTAAGAGGAAGTTGATACCTCCGGAAACCTTTGTTGGCATTTTGATTGCAAGATTCAGACTACAACTCACGAGACTGTACAATTTGGGTGCAAGGAAGATGGTTGTAGTAGGTGTAGGACCTCTTGGGTGTATACCCTATCAAAGGGATATAAATATTGTTGCTGATCCAGATAGCTGTGTTGAACTTACAAATCAACTAGCGCGGTCATTCAACAGCAAGCTACAGACCCTCGTCACCGAACTAAACACAAATCTAACAGGGGCCAAATTTGTTTATGCGAATGTTTATGGTATCTTCCACGACATCATTGAGAACTACAAATCATATGGTTTTGAGAATGCAAACTCCGCGTGCTGTGGATTTGGTGGGCGCTATGGGGGTCTAGGCTCATGCGGTCCTCAGCCATCAGAAGTTTGTGAGGACCGGTCTAAATATGTGTTTTGGGACGCTTACCATCCTTCTGAGGCTTGTAATATGATCATAGCAGGGCGTCTCTTGTATGGGAACTCCAGCGATATCTCACCCATGAATATTGTGCAACTCCTCGAACCTTCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

426

Amino Acids

47.47

Weight (kDa)

6.9

Isoelectric Point (pI)

34.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 91 - 403 2.9e-30 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 179, 713
Acc65I GGTACC 1 cut(s) 186
AccB1I GGYRCC 1 cut(s) 186
AccI GTMKAC 1 cut(s) 853
AccII CGCG 3 cut(s) 75, 925, 1073
AccIII TCCGGA 1 cut(s) 742
AciI CCGC 5 cut(s) 73, 413, 925, 1071, 1116
AclWI GGATC 2 cut(s) 25, 881
AcoI YGGCCR 1 cut(s) 35
AcsI RAATTY 5 cut(s) 149, 206, 258, 519, 989
AcuI CTGAAG 1 cut(s) 489
AcyI GRCGYC 1 cut(s) 1213
AfaI GTAC 2 cut(s) 188, 800
AfiI CCNNNNNNNGG 2 cut(s) 350, 864
AgeI ACCGGT 1 cut(s) 1146
AgsI TTSAA 3 cut(s) 296, 905, 934
AjuI GAANNNNNNNTTGG 2 cut(s) 738, 770
AloI GAACNNNNNNTCC 4 cut(s) 195, 227, 229, 261
AluBI AGCT 7 cut(s) 61, 134, 470, 640, 653, 897, 943
AluI AGCT 7 cut(s) 61, 134, 470, 640, 653, 897, 943
Alw21I GWGCWC 1 cut(s) 655
Alw26I GTCTC 2 cut(s) 787, 1220
AlwI GGATC 2 cut(s) 25, 881
AlwNI CAGNNNCTG 1 cut(s) 424
Aor13HI TCCGGA 1 cut(s) 742
AoxI GGCC 2 cut(s) 35, 984
ApeKI GCWGC 1 cut(s) 629
ApoI RAATTY 5 cut(s) 149, 206, 258, 519, 989
AsiGI ACCGGT 1 cut(s) 1146
Asp700I GAANNNNTTC 1 cut(s) 244
Asp718I GGTACC 1 cut(s) 186
AspLEI GCGC 2 cut(s) 925, 1095
AspS9I GGNCC 6 cut(s) 473, 675, 839, 984, 1118, 1144
AsuC2I CCSGG 1 cut(s) 376
AsuHPI GGTGA 2 cut(s) 949, 1236
AvaII GGWCC 5 cut(s) 473, 675, 839, 1118, 1144
BanI GGYRCC 1 cut(s) 186
BanII GRGCYC 1 cut(s) 655
BauI CACGAG 1 cut(s) 790
BbsI GAAGAC 1 cut(s) 606
Bbv12I GWGCWC 1 cut(s) 655
BbvCI CCTCAGC 1 cut(s) 1122
BbvI GCAGC 1 cut(s) 641
BccI CCATC 4 cut(s) 407, 814, 1135, 1185
BclI TGATCA 2 cut(s) 337, 1200
BcnI CCSGG 1 cut(s) 376
BcoDI GTCTC 2 cut(s) 787, 1220
BfaI CTAG 3 cut(s) 290, 920, 1106
BfmI CTRYAG 2 cut(s) 627, 944
BfoI RGCGCY 1 cut(s) 1096
BisI GCNGC 1 cut(s) 630
BlsI GCNGC 1 cut(s) 631
Bme1390I CCNGG 1 cut(s) 376
Bme18I GGWCC 5 cut(s) 473, 675, 839, 1118, 1144
BmgT120I GGNCC 6 cut(s) 473, 675, 839, 984, 1118, 1144
BmiI GGNNCC 4 cut(s) 188, 459, 676, 985
BmrFI CCNGG 1 cut(s) 376
BmsI GCATC 1 cut(s) 556
BoxI GACNNNNGTC 1 cut(s) 953
BpiI GAAGAC 1 cut(s) 606
BpmI CTGGAG 1 cut(s) 1216
Bpu10I CCTNAGC 1 cut(s) 1122
BpuEI CTTGAG 1 cut(s) 595
BpuMI CCSGG 1 cut(s) 376
BsaHI GRCGYC 1 cut(s) 1213
BsaJI CCNNGG 1 cut(s) 330
BsaWI WCCGGW 2 cut(s) 742, 1146
BsaXI ACNNNNNCTCC 2 cut(s) 229, 259
Bsc4I CCNNNNNNNGG 2 cut(s) 350, 864
Bse118I RCCGGY 1 cut(s) 1146
Bse1I ACTGG 2 cut(s) 476, 707
BseAI TCCGGA 1 cut(s) 742
BseDI CCNNGG 1 cut(s) 330
BseGI GGATG 3 cut(s) 340, 571, 1177
BseLI CCNNNNNNNGG 2 cut(s) 350, 864
BseMII CTCAG 4 cut(s) 476, 708, 1136, 1176
BseNI ACTGG 2 cut(s) 476, 707
BseRI GAGGAG 2 cut(s) 435, 1256
BseX3I CGGCCG 1 cut(s) 35
BseXI GCAGC 1 cut(s) 641
Bsh1236I CGCG 3 cut(s) 75, 925, 1073
Bsh1285I CGRYCG 1 cut(s) 38
BshFI GGCC 2 cut(s) 37, 986
BshNI GGYRCC 1 cut(s) 186
BshTI ACCGGT 1 cut(s) 1146
BsiEI CGRYCG 1 cut(s) 38
BsiHKAI GWGCWC 1 cut(s) 655
BsiSI CCGG 4 cut(s) 34, 375, 743, 1147
BslFI GGGAC 2 cut(s) 127, 1181
BslI CCNNNNNNNGG 2 cut(s) 350, 864
BsmAI GTCTC 2 cut(s) 787, 1220
BsmBI CGTCTC 1 cut(s) 1220
BsmFI GGGAC 2 cut(s) 127, 1181
BsmI GAATGC 1 cut(s) 1066
BsnI GGCC 2 cut(s) 37, 986
Bsp1286I GDGCHC 1 cut(s) 655
Bsp13I TCCGGA 1 cut(s) 742
Bsp1407I TGTACA 1 cut(s) 798
Bsp143I GATC 5 cut(s) 30, 64, 337, 886, 1200
BspACI CCGC 5 cut(s) 73, 413, 925, 1071, 1116
BspANI GGCC 2 cut(s) 37, 986
BspCNI CTCAG 4 cut(s) 477, 709, 1135, 1177
BspEI TCCGGA 1 cut(s) 742
BspFNI CGCG 3 cut(s) 75, 925, 1073
BspLI GGNNCC 4 cut(s) 188, 459, 676, 985
BspMAI CTGCAG 1 cut(s) 631
BspPI GGATC 2 cut(s) 25, 881
BspQI GCTCTTC 1 cut(s) 643
BspT107I GGYRCC 1 cut(s) 186
BsrFI RCCGGY 1 cut(s) 1146
BsrGI TGTACA 1 cut(s) 798
BsrI ACTGG 2 cut(s) 476, 707
BssAI RCCGGY 1 cut(s) 1146
BssECI CCNNGG 1 cut(s) 330
BssMI GATC 5 cut(s) 30, 64, 337, 886, 1200
BssNAI GTATAC 1 cut(s) 854
BssNI GRCGYC 1 cut(s) 1213
BssSI CACGAG 1 cut(s) 790
BssT1I CCWWGG 1 cut(s) 330
Bst1107I GTATAC 1 cut(s) 854
Bst2BI CACGAG 1 cut(s) 790
Bst4CI ACNGT 1 cut(s) 798
Bst6I CTCTTC 2 cut(s) 643, 662
BstACI GRCGYC 1 cut(s) 1213
BstAUI TGTACA 1 cut(s) 798
BstC8I GCNNGC 3 cut(s) 54, 941, 1075
BstDEI CTNAG 5 cut(s) 485, 644, 717, 1122, 1185
BstENI CCTNNNNNAGG 1 cut(s) 862
BstF5I GGATG 3 cut(s) 340, 571, 1177
BstFNI CGCG 3 cut(s) 75, 925, 1073
BstH2I RGCGCY 1 cut(s) 1096
BstHHI GCGC 2 cut(s) 925, 1095
BstKTI GATC 5 cut(s) 33, 67, 340, 889, 1203
BstMAI GTCTC 2 cut(s) 787, 1220
BstMBI GATC 5 cut(s) 30, 64, 337, 886, 1200
BstMCI CGRYCG 1 cut(s) 38
BstMWI GCNNNNNNNGC 2 cut(s) 58, 1070
BstPAI GACNNNNGTC 1 cut(s) 953
BstSCI CCNGG 1 cut(s) 374
BstSFI CTRYAG 2 cut(s) 627, 944
BstUI CGCG 3 cut(s) 75, 925, 1073
BstV1I GCAGC 1 cut(s) 641
BstV2I GAAGAC 1 cut(s) 606
BstX2I RGATCY 1 cut(s) 30
BstYI RGATCY 1 cut(s) 30
BstZ17I GTATAC 1 cut(s) 854
BstZI CGGCCG 1 cut(s) 35
BsuRI GGCC 2 cut(s) 37, 986
BtsCI GGATG 3 cut(s) 340, 571, 1177
Cac8I GCNNGC 3 cut(s) 54, 941, 1075
CaiI CAGNNNCTG 1 cut(s) 424
CfoI GCGC 2 cut(s) 925, 1095
Cfr10I RCCGGY 1 cut(s) 1146
Cfr13I GGNCC 6 cut(s) 473, 675, 839, 984, 1118, 1144
CseI GACGC 2 cut(s) 1178, 1202
Csp6I GTAC 2 cut(s) 187, 799
CspAI ACCGGT 1 cut(s) 1146
CviAII CATG 2 cut(s) 1113, 1249
CviQI GTAC 2 cut(s) 187, 799
DdeI CTNAG 5 cut(s) 485, 644, 717, 1122, 1185
DpnI GATC 5 cut(s) 32, 66, 339, 888, 1202
DpnII GATC 5 cut(s) 30, 64, 337, 886, 1200
EaeI YGGCCR 1 cut(s) 35
EagI CGGCCG 1 cut(s) 35
Eam1104I CTCTTC 2 cut(s) 643, 662
EarI CTCTTC 2 cut(s) 643, 662
Ecl136II GAGCTC 1 cut(s) 653
EclXI CGGCCG 1 cut(s) 35
Eco130I CCWWGG 1 cut(s) 330
Eco24I GRGCYC 1 cut(s) 655
Eco32I GATATC 1 cut(s) 1240
Eco47I GGWCC 5 cut(s) 473, 675, 839, 1118, 1144
Eco52I CGGCCG 1 cut(s) 35
Eco53kI GAGCTC 1 cut(s) 653
Eco57I CTGAAG 1 cut(s) 489
EcoICRI GAGCTC 1 cut(s) 653
EcoNI CCTNNNNNAGG 1 cut(s) 862
EcoO109I RGGNCCY 1 cut(s) 839
EcoRI GAATTC 1 cut(s) 519
EcoRV GATATC 1 cut(s) 1240
EcoT14I CCWWGG 1 cut(s) 330
EcoT38I GRGCYC 1 cut(s) 655
ErhI CCWWGG 1 cut(s) 330
Esp3I CGTCTC 1 cut(s) 1220
FaeI CATG 2 cut(s) 1116, 1252
FaqI GGGAC 2 cut(s) 127, 1181
FatI CATG 2 cut(s) 1112, 1248
FauI CCCGC 1 cut(s) 406
FauNDI CATATG 1 cut(s) 1048
FbaI TGATCA 2 cut(s) 337, 1200
FblI GTMKAC 1 cut(s) 853
Fnu4HI GCNGC 1 cut(s) 630
FokI GGATG 3 cut(s) 327, 578, 1164
FriOI GRGCYC 1 cut(s) 655
Fsp4HI GCNGC 1 cut(s) 630
FspBI CTAG 3 cut(s) 290, 920, 1106
GlaI GCGC 2 cut(s) 924, 1094
GluI GCNGC 1 cut(s) 630
GsuI CTGGAG 1 cut(s) 1216
HaeII RGCGCY 1 cut(s) 1096
HaeIII GGCC 2 cut(s) 37, 986
HapII CCGG 4 cut(s) 34, 375, 743, 1147
HgaI GACGC 2 cut(s) 1178, 1202
HhaI GCGC 2 cut(s) 925, 1095
Hin1I GRCGYC 1 cut(s) 1213
Hin1II CATG 2 cut(s) 1116, 1252
Hin6I GCGC 2 cut(s) 923, 1093
HinP1I GCGC 2 cut(s) 923, 1093
HincII GTYRAC 1 cut(s) 496
HindII GTYRAC 1 cut(s) 496
HinfI GANTC 5 cut(s) 115, 284, 322, 421, 774
HpaII CCGG 4 cut(s) 34, 375, 743, 1147
HphI GGTGA 2 cut(s) 949, 1236
Hpy166II GTNNAC 3 cut(s) 441, 496, 854
Hpy188I TCNGA 7 cut(s) 199, 365, 420, 486, 779, 1132, 1186
Hpy188III TCNNGA 6 cut(s) 68, 144, 743, 790, 890, 1278
Hpy8I GTNNAC 3 cut(s) 441, 496, 854
HpyAV CCTTC 3 cut(s) 11, 194, 1191
HpyCH4III ACNGT 1 cut(s) 798
HpyF10VI GCNNNNNNNGC 2 cut(s) 58, 1070
HpyF3I CTNAG 5 cut(s) 485, 644, 717, 1122, 1185
Hsp92I GRCGYC 1 cut(s) 1213
Hsp92II CATG 2 cut(s) 1116, 1252
HspAI GCGC 2 cut(s) 923, 1093
Kpn2I TCCGGA 1 cut(s) 742
KpnI GGTACC 1 cut(s) 190
Ksp22I TGATCA 2 cut(s) 337, 1200
Kzo9I GATC 5 cut(s) 30, 64, 337, 886, 1200
LguI GCTCTTC 1 cut(s) 643
LmnI GCTCC 1 cut(s) 463
Lsp1109I GCAGC 1 cut(s) 641
LweI GCATC 1 cut(s) 556
MaeI CTAG 3 cut(s) 290, 920, 1106
MaeIII GTNAC 3 cut(s) 319, 664, 955
MalI GATC 5 cut(s) 32, 66, 339, 888, 1202
MboI GATC 5 cut(s) 30, 64, 337, 886, 1200
MboII GAAGA 7 cut(s) 137, 405, 611, 649, 660, 829, 1009
MflI RGATCY 1 cut(s) 30
MhlI GDGCHC 1 cut(s) 655
MlyI GAGTC 2 cut(s) 316, 415
MmeI TCCRAC 1 cut(s) 702
MroI TCCGGA 1 cut(s) 742
MroXI GAANNNNTTC 1 cut(s) 244
MseI TTAA 3 cut(s) 431, 525, 723
MspA1I CMGCKG 1 cut(s) 470
MspI CCGG 4 cut(s) 34, 375, 743, 1147
MspR9I CCNGG 1 cut(s) 376
Mva1269I GAATGC 1 cut(s) 1066
MvnI CGCG 3 cut(s) 75, 925, 1073
MwoI GCNNNNNNNGC 2 cut(s) 58, 1070
NciI CCSGG 1 cut(s) 376
NdeI CATATG 1 cut(s) 1048
NdeII GATC 5 cut(s) 30, 64, 337, 886, 1200
NlaIII CATG 2 cut(s) 1116, 1252
NlaIV GGNNCC 4 cut(s) 188, 459, 676, 985
NmuCI GTSAC 2 cut(s) 319, 955
PciSI GCTCTTC 1 cut(s) 643
PcsI WCGNNNNNNNCGW 1 cut(s) 678
PctI GAATGC 1 cut(s) 1066
PdmI GAANNNNTTC 1 cut(s) 244
PfeI GAWTC 3 cut(s) 115, 284, 774
PinAI ACCGGT 1 cut(s) 1146
PkrI GCNGC 1 cut(s) 631
PleI GAGTC 2 cut(s) 316, 415
PpsI GAGTC 2 cut(s) 316, 415
PpuMI RGGWCCY 1 cut(s) 839
PshAI GACNNNNGTC 1 cut(s) 953
PsiI TTATAA 2 cut(s) 179, 713
Psp124BI GAGCTC 1 cut(s) 655
Psp5II RGGWCCY 1 cut(s) 839
PspN4I GGNNCC 4 cut(s) 188, 459, 676, 985
PspPI GGNCC 6 cut(s) 473, 675, 839, 984, 1118, 1144
PspPPI RGGWCCY 1 cut(s) 839
PstI CTGCAG 1 cut(s) 631
PstNI CAGNNNCTG 1 cut(s) 424
PsuI RGATCY 1 cut(s) 30
PvuII CAGCTG 1 cut(s) 470
RsaI GTAC 2 cut(s) 188, 800
RsaNI GTAC 2 cut(s) 187, 799
SacI GAGCTC 1 cut(s) 655
SapI GCTCTTC 1 cut(s) 643
SaqAI TTAA 3 cut(s) 431, 525, 723
SatI GCNGC 1 cut(s) 630
Sau3AI GATC 5 cut(s) 30, 64, 337, 886, 1200
Sau96I GGNCC 6 cut(s) 473, 675, 839, 984, 1118, 1144
SchI GAGTC 2 cut(s) 316, 415
ScrFI CCNGG 1 cut(s) 376
SduI GDGCHC 1 cut(s) 655
SfaNI GCATC 1 cut(s) 556
SfcI CTRYAG 2 cut(s) 627, 944
SinI GGWCC 5 cut(s) 473, 675, 839, 1118, 1144
SmlI CTYRAG 1 cut(s) 610
SmoI CTYRAG 1 cut(s) 610
SsiI CCGC 5 cut(s) 73, 413, 925, 1071, 1116
SspI AATATT 2 cut(s) 877, 1255
SspMI CTAG 3 cut(s) 290, 920, 1106
SstI GAGCTC 1 cut(s) 655
StyD4I CCNGG 1 cut(s) 374
StyI CCWWGG 1 cut(s) 330
TaaI ACNGT 1 cut(s) 798
TaqI TCGA 4 cut(s) 63, 90, 109, 1269
TatI WGTACW 1 cut(s) 798
TfiI GAWTC 3 cut(s) 115, 284, 774
Tru1I TTAA 3 cut(s) 431, 525, 723
Tru9I TTAA 3 cut(s) 431, 525, 723
TseFI GTSAC 2 cut(s) 319, 955
TseI GCWGC 1 cut(s) 629
Tsp45I GTSAC 2 cut(s) 319, 955
TspDTI ATGAA 1 cut(s) 1265
VpaK11BI GGWCC 5 cut(s) 473, 675, 839, 1118, 1144
XagI CCTNNNNNAGG 1 cut(s) 862
XapI RAATTY 5 cut(s) 149, 206, 258, 519, 989
XmiI GTMKAC 1 cut(s) 853
XmnI GAANNNNTTC 1 cut(s) 244
XspI CTAG 3 cut(s) 290, 920, 1106
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.