FvH4_4g19871

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0001879
Physical Location & Seq
Forward (+)
23365330 .. 23372135
6806 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g19871.t1

Sequence Viewer

Length: 1239 bp
ATGCTCTCAATAACGTGTTCGATATTTTATCTACATAAACAGGCCGGGCTGTTACTTCTTTCAAGCTTCAAGCTTCATCTGAAAACTAGTATTGGTTTTGAGAATTCTGAGCTACTACTCTTTTACAAGGTCTTTGTAATGGGTGGCCTATTGTCGGATAGAAAATTCATTCTTGGCATTATTTTTGCACTGTGGGGAATATGTTCAGCAACAGCAACTCCTGCTAATTTTGTGTTTGGAGATTCCCTAGTTGAAGTAGGGAACAACAACTATATTGTGACACTCTCCAAGGCTAATTATTATCCCAATGGAATCGATTTCGGAAAGCCTACCGGGAGATACACCAATGGAAGAACCATCATTGACATTATAGATCAGAGTCAGGGTTTCAAGGGGTTCACTCCTCCTTACTTAGCTCCCACAACAGCTGGATCAGTAATTCTGAGGGGTGTCAACTATGCTTCAGGTGGAGCTGGAATTCTTAATGAAACAGGAAAAATATTTGGTGGCAGAATTAACATGGATGCACAGATAGATAACTTTGCGAATACCAGGCAAGACATTATCTCAGGCATTGGTGTTCCTGCAGCTCTAAAGCTACTTAACCGAGCTCTCTTCTCAGTAACAATTGGCTCGAATGATTTCATCAATAACTACTTAGCACCAATTATTTCTGAGGCTGAGAGGAAGTCGATACCCCCGGAAACCTTTGTTGGCATTTTGATTGCAAGACTCAGACTACAAATTACAAGACTATACAATTTGGGTGCAAAGAAGATTGTTATGGTAAATGTCGGACCTATTGGCTGTATACCCTATCAGAGGGAAGTGAATCGGGTAGCTGATCCAGATAGCTGTGCTGCATTTTCGAATCAATTAGCACGTTCATTCAACGCCAAGCTAAGAACCCTTGTCACCGAGCTAAACAAGAATCTTCCCGGGGCCAAATTTGTGTATGCAGATGTTTATAGCATTGTAGAAGACATCATTGACAACTACAAATCATATGGTTTCCAGAATACAAACTCTGCATGCTGCAGAGTTGCAGGGCGCTACGGGGGTTTAACTCCATGCGGTCCTCAGCCATCGAAAGTTTGCGTGAACCGATCCAAGTATGTGTTTTGGGACGCTTACCATCCTTCTGATGCTTCAAATGTGATCATAGCAAGGCGTTTCTTGTATGGGAACTCCAGTGATATTTCACCCATGAATGTAGTGCAACTCCTCCAAGCTTCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

413

Amino Acids

45.02

Weight (kDa)

9.26

Isoelectric Point (pI)

26.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 77 - 391 3.6e-33 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 811
AciI CCGC 1 cut(s) 1074
AclWI GGATC 3 cut(s) 439, 839, 1101
AcsI RAATTY 4 cut(s) 103, 164, 477, 947
AcuI CTGAAG 1 cut(s) 447
AfiI CCNNNNNNNGG 2 cut(s) 154, 822
AflIII ACRYGT 1 cut(s) 14
AgsI TTSAA 6 cut(s) 63, 70, 254, 391, 892, 1152
AhlI ACTAGT 1 cut(s) 86
AjnI CCWGG 1 cut(s) 551
AjuI GAANNNNNNNTTGG 2 cut(s) 696, 728
Alw21I GWGCWC 1 cut(s) 613
AlwI GGATC 3 cut(s) 439, 839, 1101
Ama87I CYCGRG 1 cut(s) 938
AoxI GGCC 3 cut(s) 42, 145, 942
ApeKI GCWGC 3 cut(s) 587, 860, 1035
ApoI RAATTY 4 cut(s) 103, 164, 477, 947
Asp700I GAANNNNTTC 2 cut(s) 202, 641
AspLEI GCGC 1 cut(s) 1053
AspS9I GGNCC 3 cut(s) 797, 942, 1076
AsuC2I CCSGG 5 cut(s) 46, 334, 701, 939, 940
AsuHPI GGTGA 2 cut(s) 907, 1194
AsuII TTCGAA 1 cut(s) 869
AvaI CYCGRG 1 cut(s) 938
AvaII GGWCC 2 cut(s) 797, 1076
BanII GRGCYC 1 cut(s) 613
BbsI GAAGAC 1 cut(s) 987
Bbv12I GWGCWC 1 cut(s) 613
BbvCI CCTCAGC 1 cut(s) 1080
BbvI GCAGC 3 cut(s) 599, 847, 1022
BccI CCATC 3 cut(s) 365, 1093, 1143
BciT130I CCWGG 1 cut(s) 553
BclI TGATCA 1 cut(s) 1158
BcnI CCSGG 5 cut(s) 46, 334, 701, 939, 940
BcuI ACTAGT 1 cut(s) 86
BfaI CTAG 2 cut(s) 87, 248
BfmI CTRYAG 2 cut(s) 585, 1036
BfoI RGCGCY 1 cut(s) 1054
BisI GCNGC 3 cut(s) 588, 861, 1036
BlsI GCNGC 3 cut(s) 589, 862, 1037
Bme1390I CCNGG 6 cut(s) 46, 334, 553, 701, 939, 940
Bme18I GGWCC 2 cut(s) 797, 1076
BmeT110I CYCGRG 1 cut(s) 938
BmgT120I GGNCC 3 cut(s) 797, 942, 1076
BmiI GGNNCC 1 cut(s) 943
BmrFI CCNGG 6 cut(s) 46, 334, 553, 701, 939, 940
BmsI GCATC 2 cut(s) 514, 1135
BpiI GAAGAC 1 cut(s) 987
BpmI CTGGAG 1 cut(s) 1174
Bpu10I CCTNAGC 1 cut(s) 1080
Bpu14I TTCGAA 1 cut(s) 869
BpuMI CCSGG 5 cut(s) 46, 334, 701, 939, 940
Bsa29I ATCGAT 1 cut(s) 315
BsaJI CCNNGG 4 cut(s) 288, 699, 938, 939
BsaXI ACNNNNNCTCC 2 cut(s) 202, 232
Bsc4I CCNNNNNNNGG 2 cut(s) 154, 822
Bse1I ACTGG 1 cut(s) 1191
BseBI CCWGG 1 cut(s) 553
BseCI ATCGAT 1 cut(s) 315
BseDI CCNNGG 4 cut(s) 288, 699, 938, 939
BseGI GGATG 2 cut(s) 529, 1135
BseLI CCNNNNNNNGG 2 cut(s) 154, 822
BseMII CTCAG 8 cut(s) 99, 434, 582, 633, 666, 672, 748, 1094
BseNI ACTGG 1 cut(s) 1191
BseRI GAGGAG 2 cut(s) 393, 1214
BseXI GCAGC 3 cut(s) 599, 847, 1022
BshFI GGCC 3 cut(s) 44, 147, 944
BshVI ATCGAT 1 cut(s) 315
BsiHKAI GWGCWC 1 cut(s) 613
BsiHKCI CYCGRG 1 cut(s) 938
BsiSI CCGG 4 cut(s) 45, 333, 701, 939
BslFI GGGAC 1 cut(s) 1139
BslI CCNNNNNNNGG 2 cut(s) 154, 822
BsmFI GGGAC 1 cut(s) 1139
BsnI GGCC 3 cut(s) 44, 147, 944
BsoBI CYCGRG 1 cut(s) 938
Bsp119I TTCGAA 1 cut(s) 869
Bsp1286I GDGCHC 1 cut(s) 613
Bsp143I GATC 5 cut(s) 373, 431, 844, 1106, 1158
BspACI CCGC 1 cut(s) 1074
BspANI GGCC 3 cut(s) 44, 147, 944
BspCNI CTCAG 8 cut(s) 100, 435, 581, 632, 667, 673, 747, 1093
BspDI ATCGAT 1 cut(s) 315
BspLI GGNNCC 1 cut(s) 943
BspMAI CTGCAG 2 cut(s) 589, 1040
BspPI GGATC 3 cut(s) 439, 839, 1101
BspT104I TTCGAA 1 cut(s) 869
BsrI ACTGG 1 cut(s) 1191
BssECI CCNNGG 4 cut(s) 288, 699, 938, 939
BssMI GATC 5 cut(s) 373, 431, 844, 1106, 1158
BssNAI GTATAC 1 cut(s) 812
BssT1I CCWWGG 1 cut(s) 288
Bst1107I GTATAC 1 cut(s) 812
Bst2UI CCWGG 1 cut(s) 553
Bst4CI ACNGT 1 cut(s) 192
Bst6I CTCTTC 1 cut(s) 620
BstAPI GCANNNNNTGC 1 cut(s) 221
BstBI TTCGAA 1 cut(s) 869
BstC8I GCNNGC 1 cut(s) 1033
BstENI CCTNNNNNAGG 1 cut(s) 820
BstF5I GGATG 2 cut(s) 529, 1135
BstH2I RGCGCY 1 cut(s) 1054
BstHHI GCGC 1 cut(s) 1053
BstKTI GATC 5 cut(s) 376, 434, 847, 1109, 1161
BstMBI GATC 5 cut(s) 373, 431, 844, 1106, 1158
BstMWI GCNNNNNNNGC 1 cut(s) 221
BstNI CCWGG 1 cut(s) 553
BstNSI RCATGY 1 cut(s) 1035
BstSCI CCNGG 6 cut(s) 44, 332, 551, 699, 937, 938
BstSFI CTRYAG 2 cut(s) 585, 1036
BstV1I GCAGC 3 cut(s) 599, 847, 1022
BstV2I GAAGAC 1 cut(s) 987
BstZ17I GTATAC 1 cut(s) 812
Bsu15I ATCGAT 1 cut(s) 315
BsuRI GGCC 3 cut(s) 44, 147, 944
BsuTUI ATCGAT 1 cut(s) 315
BtsCI GGATG 2 cut(s) 529, 1135
BtsIMutI CAGTG 2 cut(s) 188, 1198
Cac8I GCNNGC 1 cut(s) 1033
CfoI GCGC 1 cut(s) 1053
Cfr13I GGNCC 3 cut(s) 797, 942, 1076
Cfr9I CCCGGG 1 cut(s) 938
ClaI ATCGAT 1 cut(s) 315
CseI GACGC 1 cut(s) 1136
CviAII CATG 4 cut(s) 520, 1032, 1071, 1207
DpnI GATC 5 cut(s) 375, 433, 846, 1108, 1160
DpnII GATC 5 cut(s) 373, 431, 844, 1106, 1158
Eam1104I CTCTTC 1 cut(s) 620
EarI CTCTTC 1 cut(s) 620
Ecl136II GAGCTC 1 cut(s) 611
Eco130I CCWWGG 1 cut(s) 288
Eco24I GRGCYC 1 cut(s) 613
Eco47I GGWCC 2 cut(s) 797, 1076
Eco53kI GAGCTC 1 cut(s) 611
Eco57I CTGAAG 1 cut(s) 447
Eco88I CYCGRG 1 cut(s) 938
EcoICRI GAGCTC 1 cut(s) 611
EcoNI CCTNNNNNAGG 1 cut(s) 820
EcoRI GAATTC 2 cut(s) 103, 477
EcoRII CCWGG 1 cut(s) 551
EcoT14I CCWWGG 1 cut(s) 288
EcoT38I GRGCYC 1 cut(s) 613
ErhI CCWWGG 1 cut(s) 288
FaeI CATG 4 cut(s) 523, 1035, 1074, 1210
FaqI GGGAC 1 cut(s) 1139
FatI CATG 4 cut(s) 519, 1031, 1070, 1206
FauNDI CATATG 1 cut(s) 1006
FbaI TGATCA 1 cut(s) 1158
FblI GTMKAC 1 cut(s) 811
Fnu4HI GCNGC 3 cut(s) 588, 861, 1036
FokI GGATG 2 cut(s) 536, 1122
FriOI GRGCYC 1 cut(s) 613
Fsp4HI GCNGC 3 cut(s) 588, 861, 1036
FspBI CTAG 2 cut(s) 87, 248
GlaI GCGC 1 cut(s) 1052
GluI GCNGC 3 cut(s) 588, 861, 1036
GsuI CTGGAG 1 cut(s) 1174
HaeII RGCGCY 1 cut(s) 1054
HaeIII GGCC 3 cut(s) 44, 147, 944
HapII CCGG 4 cut(s) 45, 333, 701, 939
HgaI GACGC 1 cut(s) 1136
HhaI GCGC 1 cut(s) 1053
Hin1II CATG 4 cut(s) 523, 1035, 1074, 1210
Hin6I GCGC 1 cut(s) 1051
HinP1I GCGC 1 cut(s) 1051
HincII GTYRAC 1 cut(s) 454
HindII GTYRAC 1 cut(s) 454
HindIII AAGCTT 3 cut(s) 64, 71, 1230
HinfI GANTC 7 cut(s) 242, 312, 379, 732, 832, 871, 931
HpaII CCGG 4 cut(s) 45, 333, 701, 939
HphI GGTGA 2 cut(s) 907, 1194
Hpy166II GTNNAC 4 cut(s) 399, 454, 812, 1102
Hpy188III TCNNGA 3 cut(s) 848, 1015, 1236
Hpy8I GTNNAC 4 cut(s) 399, 454, 812, 1102
HpyAV CCTTC 1 cut(s) 1149
HpyCH4III ACNGT 1 cut(s) 192
HpyCH4IV ACGT 2 cut(s) 14, 883
HpyF10VI GCNNNNNNNGC 1 cut(s) 221
HpySE526I ACGT 2 cut(s) 14, 883
Hsp92II CATG 4 cut(s) 523, 1035, 1074, 1210
HspAI GCGC 1 cut(s) 1051
Ksp22I TGATCA 1 cut(s) 1158
Kzo9I GATC 5 cut(s) 373, 431, 844, 1106, 1158
LmnI GCTCC 2 cut(s) 421, 470
Lsp1109I GCAGC 3 cut(s) 599, 847, 1022
LweI GCATC 2 cut(s) 514, 1135
MaeI CTAG 2 cut(s) 87, 248
MaeII ACGT 2 cut(s) 14, 883
MaeIII GTNAC 4 cut(s) 51, 277, 622, 913
MalI GATC 5 cut(s) 375, 433, 846, 1108, 1160
MboI GATC 5 cut(s) 373, 431, 844, 1106, 1158
MboII GAAGA 5 cut(s) 363, 607, 787, 926, 992
MfeI CAATTG 1 cut(s) 627
MhlI GDGCHC 1 cut(s) 613
MlyI GAGTC 2 cut(s) 388, 726
MmeI TCCRAC 2 cut(s) 135, 775
MnlI CCTC 7 cut(s) 414, 438, 670, 678, 816, 1089, 1235
MroXI GAANNNNTTC 2 cut(s) 202, 641
MseI TTAA 4 cut(s) 483, 516, 603, 1064
MspA1I CMGCKG 1 cut(s) 428
MspI CCGG 4 cut(s) 45, 333, 701, 939
MspR9I CCNGG 6 cut(s) 46, 334, 553, 701, 939, 940
MunI CAATTG 1 cut(s) 627
MvaI CCWGG 1 cut(s) 553
MwoI GCNNNNNNNGC 1 cut(s) 221
NciI CCSGG 5 cut(s) 46, 334, 701, 939, 940
NdeI CATATG 1 cut(s) 1006
NdeII GATC 5 cut(s) 373, 431, 844, 1106, 1158
NlaIII CATG 4 cut(s) 523, 1035, 1074, 1210
NlaIV GGNNCC 1 cut(s) 943
NmuCI GTSAC 2 cut(s) 277, 913
NspI RCATGY 1 cut(s) 1035
NspV TTCGAA 1 cut(s) 869
PaeI GCATGC 1 cut(s) 1035
PdmI GAANNNNTTC 2 cut(s) 202, 641
PfeI GAWTC 5 cut(s) 242, 312, 832, 871, 931
PkrI GCNGC 3 cut(s) 589, 862, 1037
PleI GAGTC 2 cut(s) 387, 726
PpsI GAGTC 2 cut(s) 387, 726
Psp124BI GAGCTC 1 cut(s) 613
Psp6I CCWGG 1 cut(s) 551
PspGI CCWGG 1 cut(s) 551
PspN4I GGNNCC 1 cut(s) 943
PspPI GGNCC 3 cut(s) 797, 942, 1076
PstI CTGCAG 2 cut(s) 589, 1040
PvuII CAGCTG 1 cut(s) 428
SacI GAGCTC 1 cut(s) 613
SaqAI TTAA 4 cut(s) 483, 516, 603, 1064
SatI GCNGC 3 cut(s) 588, 861, 1036
Sau3AI GATC 5 cut(s) 373, 431, 844, 1106, 1158
Sau96I GGNCC 3 cut(s) 797, 942, 1076
SchI GAGTC 2 cut(s) 388, 726
ScrFI CCNGG 6 cut(s) 46, 334, 553, 701, 939, 940
SduI GDGCHC 1 cut(s) 613
SfaNI GCATC 2 cut(s) 514, 1135
SfcI CTRYAG 2 cut(s) 585, 1036
SfuI TTCGAA 1 cut(s) 869
SinI GGWCC 2 cut(s) 797, 1076
SmaI CCCGGG 1 cut(s) 940
SpeI ACTAGT 1 cut(s) 86
SphI GCATGC 1 cut(s) 1035
SsiI CCGC 1 cut(s) 1074
SspI AATATT 1 cut(s) 501
SspMI CTAG 2 cut(s) 87, 248
SstI GAGCTC 1 cut(s) 613
StyD4I CCNGG 6 cut(s) 44, 332, 551, 699, 937, 938
StyI CCWWGG 1 cut(s) 288
TaaI ACNGT 1 cut(s) 192
TaiI ACGT 2 cut(s) 17, 886
TaqI TCGA 6 cut(s) 20, 315, 635, 692, 869, 1088
TfiI GAWTC 5 cut(s) 242, 312, 832, 871, 931
Tru1I TTAA 4 cut(s) 483, 516, 603, 1064
Tru9I TTAA 4 cut(s) 483, 516, 603, 1064
TscAI CASTG 2 cut(s) 195, 1198
TseFI GTSAC 2 cut(s) 277, 913
TseI GCWGC 3 cut(s) 587, 860, 1035
Tsp45I GTSAC 2 cut(s) 277, 913
TspDTI ATGAA 6 cut(s) 65, 157, 501, 634, 876, 1223
TspMI CCCGGG 1 cut(s) 938
TspRI CASTG 2 cut(s) 195, 1198
VpaK11BI GGWCC 2 cut(s) 797, 1076
XagI CCTNNNNNAGG 1 cut(s) 820
XapI RAATTY 4 cut(s) 103, 164, 477, 947
XceI RCATGY 1 cut(s) 1035
XmaI CCCGGG 1 cut(s) 938
XmiI GTMKAC 1 cut(s) 811
XmnI GAANNNNTTC 2 cut(s) 202, 641
XspI CTAG 2 cut(s) 87, 248
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.