RLG00000007369

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr2
OG0001879
Physical Location & Seq
Reverse (-)
16176755 .. 16177861
1107 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000007369

Sequence Viewer

Length: 744 bp
ATGCTTCAGATTGGTAGAATAAACTTGGATGCACAGATAGATAACTTTGCAAATACCAGAGAAGACATTATCTCAAGCATTGGTCGTCCTGCAGCAGTAGAGCTACTTAGAAGAGCTCTCTTCTCGGTAACAATCGGGTCAAACGATTTCCTCAATAACTACTTGTTGCCAGTTATAACTGAGTTTAAGAGGAAGTTGATACCTCCGGAAACCTTTGTTGGCATTTTGATTGCAAGATTCAGACTACAACTCACAAGACTGTACTATTTGGGTGCAAGGAAGATGGTTGTAGTAGGTGTAGGACCTCTTGGGTGTATACCCTATCAAAGGGATATAAATATTGTTGCTGATCCAGATAGCTGTGTTGAACTTACAAATCAACTAGCGCGGTCATTCAACAGCAAGCTACAGACCCTCGTCACCGAACTAAACACAAATCTAACAGGGGCCAAATTTGTTTATGCGAATGTTTATGGTATCTTCCACGACATCATTGAGAACTACAAATCATATGGTTTTGAGAATGAAAACTCCGCGTGCTGCAGATCTGGTGGGCGCTATGGGGGTCTAGGCTCATGCGGTCCTCAGCCATCAGAAGTTTGTGAGGACCGGTCTAAGTATGTGTTTTGGGACGCTTACCATCCTTCTGAGGCTTGTAATATGATCATAGCAGGGCGTCTCTTGTATGGGAACTCCAGCGATATTTCACCCATGAATATTGTGCAACTCCTCCAATCTTCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

248

Amino Acids

27.51

Weight (kDa)

6.72

Isoelectric Point (pI)

48.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 37 - 224 1.9e-22 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 176
AccI GTMKAC 1 cut(s) 316
AccII CGCG 2 cut(s) 388, 536
AccIII TCCGGA 1 cut(s) 205
AciI CCGC 3 cut(s) 388, 534, 579
AclWI GGATC 1 cut(s) 344
AcsI RAATTY 1 cut(s) 452
AcyI GRCGYC 1 cut(s) 676
AfaI GTAC 1 cut(s) 263
AfiI CCNNNNNNNGG 1 cut(s) 327
AgeI ACCGGT 1 cut(s) 609
AgsI TTSAA 2 cut(s) 368, 397
AjuI GAANNNNNNNTTGG 2 cut(s) 201, 233
AluBI AGCT 4 cut(s) 103, 116, 360, 406
AluI AGCT 4 cut(s) 103, 116, 360, 406
Alw21I GWGCWC 1 cut(s) 118
Alw26I GTCTC 1 cut(s) 683
AlwI GGATC 1 cut(s) 344
Aor13HI TCCGGA 1 cut(s) 205
AoxI GGCC 1 cut(s) 447
ApeKI GCWGC 2 cut(s) 92, 540
ApoI RAATTY 1 cut(s) 452
AsiGI ACCGGT 1 cut(s) 609
AspLEI GCGC 2 cut(s) 388, 558
AspS9I GGNCC 4 cut(s) 302, 447, 581, 607
AsuHPI GGTGA 2 cut(s) 412, 699
AvaII GGWCC 3 cut(s) 302, 581, 607
BanII GRGCYC 1 cut(s) 118
BbsI GAAGAC 1 cut(s) 69
Bbv12I GWGCWC 1 cut(s) 118
BbvCI CCTCAGC 1 cut(s) 585
BbvI GCAGC 2 cut(s) 104, 527
BccI CCATC 3 cut(s) 277, 598, 648
BclI TGATCA 1 cut(s) 663
BcoDI GTCTC 1 cut(s) 683
BfaI CTAG 2 cut(s) 383, 569
BfmI CTRYAG 3 cut(s) 90, 407, 541
BfoI RGCGCY 1 cut(s) 559
BglII AGATCT 1 cut(s) 545
BisI GCNGC 2 cut(s) 93, 541
BlsI GCNGC 2 cut(s) 94, 542
Bme18I GGWCC 3 cut(s) 302, 581, 607
BmgT120I GGNCC 4 cut(s) 302, 447, 581, 607
BmiI GGNNCC 1 cut(s) 448
BmsI GCATC 1 cut(s) 19
BoxI GACNNNNGTC 1 cut(s) 416
BpiI GAAGAC 1 cut(s) 69
BpmI CTGGAG 1 cut(s) 679
Bpu10I CCTNAGC 1 cut(s) 585
BpuEI CTTGAG 1 cut(s) 58
BsaHI GRCGYC 1 cut(s) 676
BsaWI WCCGGW 2 cut(s) 205, 609
Bsc4I CCNNNNNNNGG 1 cut(s) 327
Bse118I RCCGGY 1 cut(s) 609
Bse1I ACTGG 1 cut(s) 170
BseAI TCCGGA 1 cut(s) 205
BseGI GGATG 2 cut(s) 34, 640
BseLI CCNNNNNNNGG 1 cut(s) 327
BseMII CTCAG 3 cut(s) 171, 599, 639
BseNI ACTGG 1 cut(s) 170
BseRI GAGGAG 1 cut(s) 719
BseXI GCAGC 2 cut(s) 104, 527
Bsh1236I CGCG 2 cut(s) 388, 536
BshFI GGCC 1 cut(s) 449
BshTI ACCGGT 1 cut(s) 609
BsiHKAI GWGCWC 1 cut(s) 118
BsiSI CCGG 2 cut(s) 206, 610
BslFI GGGAC 1 cut(s) 644
BslI CCNNNNNNNGG 1 cut(s) 327
BsmAI GTCTC 1 cut(s) 683
BsmBI CGTCTC 1 cut(s) 683
BsmFI GGGAC 1 cut(s) 644
BsnI GGCC 1 cut(s) 449
Bsp1286I GDGCHC 1 cut(s) 118
Bsp13I TCCGGA 1 cut(s) 205
Bsp143I GATC 3 cut(s) 349, 545, 663
BspACI CCGC 3 cut(s) 388, 534, 579
BspANI GGCC 1 cut(s) 449
BspCNI CTCAG 3 cut(s) 172, 598, 640
BspEI TCCGGA 1 cut(s) 205
BspFNI CGCG 2 cut(s) 388, 536
BspLI GGNNCC 1 cut(s) 448
BspMAI CTGCAG 2 cut(s) 94, 545
BspPI GGATC 1 cut(s) 344
BspQI GCTCTTC 1 cut(s) 106
BsrFI RCCGGY 1 cut(s) 609
BsrI ACTGG 1 cut(s) 170
BssAI RCCGGY 1 cut(s) 609
BssMI GATC 3 cut(s) 349, 545, 663
BssNAI GTATAC 1 cut(s) 317
BssNI GRCGYC 1 cut(s) 676
Bst1107I GTATAC 1 cut(s) 317
Bst4CI ACNGT 1 cut(s) 261
Bst6I CTCTTC 2 cut(s) 106, 125
BstACI GRCGYC 1 cut(s) 676
BstC8I GCNNGC 2 cut(s) 404, 538
BstDEI CTNAG 5 cut(s) 107, 180, 585, 615, 648
BstENI CCTNNNNNAGG 1 cut(s) 325
BstF5I GGATG 2 cut(s) 34, 640
BstFNI CGCG 2 cut(s) 388, 536
BstH2I RGCGCY 1 cut(s) 559
BstHHI GCGC 2 cut(s) 388, 558
BstKTI GATC 3 cut(s) 352, 548, 666
BstMAI GTCTC 1 cut(s) 683
BstMBI GATC 3 cut(s) 349, 545, 663
BstPAI GACNNNNGTC 1 cut(s) 416
BstSFI CTRYAG 3 cut(s) 90, 407, 541
BstUI CGCG 2 cut(s) 388, 536
BstV1I GCAGC 2 cut(s) 104, 527
BstV2I GAAGAC 1 cut(s) 69
BstX2I RGATCY 1 cut(s) 545
BstYI RGATCY 1 cut(s) 545
BstZ17I GTATAC 1 cut(s) 317
BsuRI GGCC 1 cut(s) 449
BtsCI GGATG 2 cut(s) 34, 640
Cac8I GCNNGC 2 cut(s) 404, 538
CfoI GCGC 2 cut(s) 388, 558
Cfr10I RCCGGY 1 cut(s) 609
Cfr13I GGNCC 4 cut(s) 302, 447, 581, 607
CseI GACGC 2 cut(s) 641, 665
Csp6I GTAC 1 cut(s) 262
CspAI ACCGGT 1 cut(s) 609
CviAII CATG 2 cut(s) 576, 712
CviJI RGCY 8 cut(s) 103, 116, 360, 406, 449, 573, 589, 653
CviKI_1 RGCY 8 cut(s) 103, 116, 360, 406, 449, 573, 589, 653
CviQI GTAC 1 cut(s) 262
DdeI CTNAG 5 cut(s) 107, 180, 585, 615, 648
DpnI GATC 3 cut(s) 351, 547, 665
DpnII GATC 3 cut(s) 349, 545, 663
Eam1104I CTCTTC 2 cut(s) 106, 125
EarI CTCTTC 2 cut(s) 106, 125
Ecl136II GAGCTC 1 cut(s) 116
Eco24I GRGCYC 1 cut(s) 118
Eco47I GGWCC 3 cut(s) 302, 581, 607
Eco53kI GAGCTC 1 cut(s) 116
EcoICRI GAGCTC 1 cut(s) 116
EcoNI CCTNNNNNAGG 1 cut(s) 325
EcoO109I RGGNCCY 1 cut(s) 302
EcoT38I GRGCYC 1 cut(s) 118
Esp3I CGTCTC 1 cut(s) 683
FaeI CATG 2 cut(s) 579, 715
FaqI GGGAC 1 cut(s) 644
FatI CATG 2 cut(s) 575, 711
FauNDI CATATG 1 cut(s) 511
FbaI TGATCA 1 cut(s) 663
FblI GTMKAC 1 cut(s) 316
Fnu4HI GCNGC 2 cut(s) 93, 541
FokI GGATG 2 cut(s) 41, 627
FriOI GRGCYC 1 cut(s) 118
Fsp4HI GCNGC 2 cut(s) 93, 541
FspBI CTAG 2 cut(s) 383, 569
GlaI GCGC 2 cut(s) 387, 557
GluI GCNGC 2 cut(s) 93, 541
GsuI CTGGAG 1 cut(s) 679
HaeII RGCGCY 1 cut(s) 559
HaeIII GGCC 1 cut(s) 449
HapII CCGG 2 cut(s) 206, 610
HgaI GACGC 2 cut(s) 641, 665
HhaI GCGC 2 cut(s) 388, 558
Hin1I GRCGYC 1 cut(s) 676
Hin1II CATG 2 cut(s) 579, 715
Hin6I GCGC 2 cut(s) 386, 556
HinP1I GCGC 2 cut(s) 386, 556
HinfI GANTC 1 cut(s) 237
HpaII CCGG 2 cut(s) 206, 610
HphI GGTGA 2 cut(s) 412, 699
Hpy166II GTNNAC 1 cut(s) 317
Hpy188I TCNGA 4 cut(s) 9, 242, 595, 649
Hpy188III TCNNGA 3 cut(s) 206, 353, 741
Hpy8I GTNNAC 1 cut(s) 317
HpyAV CCTTC 1 cut(s) 654
HpyCH4III ACNGT 1 cut(s) 261
HpyCH4V TGCA 7 cut(s) 32, 50, 92, 233, 275, 543, 724
HpyF3I CTNAG 5 cut(s) 107, 180, 585, 615, 648
Hsp92I GRCGYC 1 cut(s) 676
Hsp92II CATG 2 cut(s) 579, 715
HspAI GCGC 2 cut(s) 386, 556
Kpn2I TCCGGA 1 cut(s) 205
Ksp22I TGATCA 1 cut(s) 663
Kzo9I GATC 3 cut(s) 349, 545, 663
LguI GCTCTTC 1 cut(s) 106
Lsp1109I GCAGC 2 cut(s) 104, 527
LweI GCATC 1 cut(s) 19
MaeI CTAG 2 cut(s) 383, 569
MaeIII GTNAC 2 cut(s) 127, 418
MalI GATC 3 cut(s) 351, 547, 665
MboI GATC 3 cut(s) 349, 545, 663
MboII GAAGA 6 cut(s) 74, 112, 123, 292, 472, 729
MflI RGATCY 1 cut(s) 545
MhlI GDGCHC 1 cut(s) 118
MluCI AATT 1 cut(s) 452
MnlI CCTC 9 cut(s) 161, 183, 213, 315, 425, 594, 598, 643, 740
MroI TCCGGA 1 cut(s) 205
MseI TTAA 1 cut(s) 186
MspI CCGG 2 cut(s) 206, 610
MvnI CGCG 2 cut(s) 388, 536
NdeI CATATG 1 cut(s) 511
NdeII GATC 3 cut(s) 349, 545, 663
NlaIII CATG 2 cut(s) 579, 715
NlaIV GGNNCC 1 cut(s) 448
NmuCI GTSAC 1 cut(s) 418
PciSI GCTCTTC 1 cut(s) 106
PcsI WCGNNNNNNNCGW 1 cut(s) 141
PfeI GAWTC 1 cut(s) 237
PinAI ACCGGT 1 cut(s) 609
PkrI GCNGC 2 cut(s) 94, 542
PpuMI RGGWCCY 1 cut(s) 302
PshAI GACNNNNGTC 1 cut(s) 416
PsiI TTATAA 1 cut(s) 176
Psp124BI GAGCTC 1 cut(s) 118
Psp5II RGGWCCY 1 cut(s) 302
PspN4I GGNNCC 1 cut(s) 448
PspPI GGNCC 4 cut(s) 302, 447, 581, 607
PspPPI RGGWCCY 1 cut(s) 302
PstI CTGCAG 2 cut(s) 94, 545
PsuI RGATCY 1 cut(s) 545
RsaI GTAC 1 cut(s) 263
RsaNI GTAC 1 cut(s) 262
SacI GAGCTC 1 cut(s) 118
SapI GCTCTTC 1 cut(s) 106
SaqAI TTAA 1 cut(s) 186
SatI GCNGC 2 cut(s) 93, 541
Sau3AI GATC 3 cut(s) 349, 545, 663
Sau96I GGNCC 4 cut(s) 302, 447, 581, 607
SduI GDGCHC 1 cut(s) 118
SetI ASST 8 cut(s) 105, 118, 205, 215, 298, 307, 362, 408
SfaNI GCATC 1 cut(s) 19
SfcI CTRYAG 3 cut(s) 90, 407, 541
SinI GGWCC 3 cut(s) 302, 581, 607
SmlI CTYRAG 1 cut(s) 73
SmoI CTYRAG 1 cut(s) 73
Sse9I AATT 1 cut(s) 452
SsiI CCGC 3 cut(s) 388, 534, 579
SspI AATATT 2 cut(s) 340, 718
SspMI CTAG 2 cut(s) 383, 569
SstI GAGCTC 1 cut(s) 118
TaaI ACNGT 1 cut(s) 261
TasI AATT 1 cut(s) 452
TatI WGTACW 1 cut(s) 261
TfiI GAWTC 1 cut(s) 237
Tru1I TTAA 1 cut(s) 186
Tru9I TTAA 1 cut(s) 186
TseFI GTSAC 1 cut(s) 418
TseI GCWGC 2 cut(s) 92, 540
Tsp45I GTSAC 1 cut(s) 418
TspDTI ATGAA 2 cut(s) 540, 728
VpaK11BI GGWCC 3 cut(s) 302, 581, 607
XagI CCTNNNNNAGG 1 cut(s) 325
XapI RAATTY 1 cut(s) 452
XmiI GTMKAC 1 cut(s) 316
XspI CTAG 2 cut(s) 383, 569
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.