Rh4CG277300

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4C
OG0001879
Physical Location & Seq
Forward (+)
53355591 .. 53356489
899 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4CG277300.1

Sequence Viewer

Length: 573 bp
ATGTGTTCAGCAAAAGAAACTCCTGCTAATTTTGTGTTTGTCGATTCTCTAGTTGAAGTGGGGAACAACAACTACATTGTGACTCTCTCCAAGGCTGATTATCCTCCCAATGGAATCGATTTCGGAAAGCCTACCGGGAGATACACAAATGGAAGAACCATCATTGACATTATAGGCCAGACTCTTGGTTTTAAGGGGTTCACTCCTCCTTACTTAGCTCCCACAACAGCTGGACCAGTAATTCTGAGCGGTGTTAACTATGCTTCAGGTGGAGGTGGAATTCTTAATGAAACAGGAAAAATATTTGTTGGTAGAATAAACTTGGAAGCACAGACAGATAACTTTGCAAATACCAGGCAAGATATTATCTCAAGAATTGGTCTTCATGCAGCTATAAAGCTACTTAGAAGTGCTCTGTTCTCACTAACAATCGGCTCGAATGATTCCATCAATAACTACTTGGCGCCAGTTATATCTGAGGCTGAGAGGAAGTCGATACCTCCGGAAACCTTTGTTGGCATTTTGATCGCAAGACTCAGACTACAAATTACAGTAGACATTTTTCATTCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

190

Amino Acids

20.4

Weight (kDa)

7.84

Isoelectric Point (pI)

22.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 15 - 178 2.2e-06 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 463
AccBSI CCGCTC 1 cut(s) 249
AccI GTMKAC 1 cut(s) 555
AccIII TCCGGA 1 cut(s) 502
AciI CCGC 1 cut(s) 249
AcsI RAATTY 1 cut(s) 279
AcuI CTGAAG 1 cut(s) 249
AcyI GRCGYC 1 cut(s) 464
AfiI CCNNNNNNNGG 1 cut(s) 110
AgsI TTSAA 1 cut(s) 56
AjnI CCWGG 1 cut(s) 353
AjuI GAANNNNNNNTTGG 2 cut(s) 498, 530
AluBI AGCT 4 cut(s) 218, 230, 392, 400
AluI AGCT 4 cut(s) 218, 230, 392, 400
Alw21I GWGCWC 1 cut(s) 415
Aor13HI TCCGGA 1 cut(s) 502
AoxI GGCC 1 cut(s) 175
ApeKI GCWGC 1 cut(s) 389
ApoI RAATTY 1 cut(s) 279
AspLEI GCGC 1 cut(s) 466
AspS9I GGNCC 1 cut(s) 233
AsuC2I CCSGG 1 cut(s) 136
AvaII GGWCC 1 cut(s) 233
BanI GGYRCC 1 cut(s) 463
BbsI GAAGAC 1 cut(s) 374
Bbv12I GWGCWC 1 cut(s) 415
BbvI GCAGC 1 cut(s) 401
BccI CCATC 2 cut(s) 167, 455
BcgI CGANNNNNNTGC 2 cut(s) 508, 542
BciT130I CCWGG 1 cut(s) 355
BcnI CCSGG 1 cut(s) 136
BfaI CTAG 1 cut(s) 50
BfoI RGCGCY 1 cut(s) 467
BisI GCNGC 1 cut(s) 390
BlsI GCNGC 1 cut(s) 391
Bme1390I CCNGG 2 cut(s) 136, 355
Bme18I GGWCC 1 cut(s) 233
BmgT120I GGNCC 1 cut(s) 233
BmiI GGNNCC 1 cut(s) 465
BmrFI CCNGG 2 cut(s) 136, 355
BpiI GAAGAC 1 cut(s) 374
BpuEI CTTGAG 1 cut(s) 355
BpuMI CCSGG 1 cut(s) 136
Bsa29I ATCGAT 1 cut(s) 117
BsaHI GRCGYC 1 cut(s) 464
BsaJI CCNNGG 1 cut(s) 90
BsaWI WCCGGW 1 cut(s) 502
Bsc4I CCNNNNNNNGG 1 cut(s) 110
Bse1I ACTGG 2 cut(s) 236, 467
BseAI TCCGGA 1 cut(s) 502
BseBI CCWGG 1 cut(s) 355
BseCI ATCGAT 1 cut(s) 117
BseDI CCNNGG 1 cut(s) 90
BseLI CCNNNNNNNGG 1 cut(s) 110
BseMII CTCAG 4 cut(s) 236, 468, 474, 550
BseNI ACTGG 2 cut(s) 236, 467
BseRI GAGGAG 1 cut(s) 195
BseXI GCAGC 1 cut(s) 401
BshFI GGCC 1 cut(s) 177
BshNI GGYRCC 1 cut(s) 463
BshVI ATCGAT 1 cut(s) 117
BsiHKAI GWGCWC 1 cut(s) 415
BsiSI CCGG 2 cut(s) 135, 503
BslI CCNNNNNNNGG 1 cut(s) 110
BsnI GGCC 1 cut(s) 177
Bsp1286I GDGCHC 1 cut(s) 415
Bsp13I TCCGGA 1 cut(s) 502
Bsp143I GATC 1 cut(s) 525
BspACI CCGC 1 cut(s) 249
BspANI GGCC 1 cut(s) 177
BspCNI CTCAG 4 cut(s) 237, 469, 475, 549
BspDI ATCGAT 1 cut(s) 117
BspEI TCCGGA 1 cut(s) 502
BspLI GGNNCC 1 cut(s) 465
BspT107I GGYRCC 1 cut(s) 463
BsrBI CCGCTC 1 cut(s) 249
BsrI ACTGG 2 cut(s) 236, 467
BssECI CCNNGG 1 cut(s) 90
BssMI GATC 1 cut(s) 525
BssNI GRCGYC 1 cut(s) 464
BssT1I CCWWGG 1 cut(s) 90
Bst2UI CCWGG 1 cut(s) 355
Bst4CI ACNGT 1 cut(s) 553
BstACI GRCGYC 1 cut(s) 464
BstDEI CTNAG 6 cut(s) 214, 245, 404, 477, 483, 536
BstH2I RGCGCY 1 cut(s) 467
BstHHI GCGC 1 cut(s) 466
BstKTI GATC 1 cut(s) 528
BstMBI GATC 1 cut(s) 525
BstNI CCWGG 1 cut(s) 355
BstSCI CCNGG 2 cut(s) 134, 353
BstV1I GCAGC 1 cut(s) 401
BstV2I GAAGAC 1 cut(s) 374
BstXI CCANNNNNNTGG 1 cut(s) 185
Bsu15I ATCGAT 1 cut(s) 117
BsuRI GGCC 1 cut(s) 177
BsuTUI ATCGAT 1 cut(s) 117
CfoI GCGC 1 cut(s) 466
Cfr13I GGNCC 1 cut(s) 233
ClaI ATCGAT 1 cut(s) 117
CviAII CATG 1 cut(s) 386
CviJI RGCY 9 cut(s) 95, 130, 177, 218, 230, 392, 400, 435, 482
CviKI_1 RGCY 9 cut(s) 95, 130, 177, 218, 230, 392, 400, 435, 482
DdeI CTNAG 6 cut(s) 214, 245, 404, 477, 483, 536
DinI GGCGCC 1 cut(s) 465
DpnI GATC 1 cut(s) 527
DpnII GATC 1 cut(s) 525
Eco130I CCWWGG 1 cut(s) 90
Eco47I GGWCC 1 cut(s) 233
Eco57I CTGAAG 1 cut(s) 249
EcoRI GAATTC 1 cut(s) 279
EcoRII CCWGG 1 cut(s) 353
EcoT14I CCWWGG 1 cut(s) 90
EgeI GGCGCC 1 cut(s) 465
EheI GGCGCC 1 cut(s) 465
ErhI CCWWGG 1 cut(s) 90
FaeI CATG 1 cut(s) 389
FaiI YATR 6 cut(s) 173, 261, 387, 395, 473, 571
FatI CATG 1 cut(s) 385
FblI GTMKAC 1 cut(s) 555
Fnu4HI GCNGC 1 cut(s) 390
Fsp4HI GCNGC 1 cut(s) 390
FspBI CTAG 1 cut(s) 50
GlaI GCGC 1 cut(s) 465
GluI GCNGC 1 cut(s) 390
HaeII RGCGCY 1 cut(s) 467
HaeIII GGCC 1 cut(s) 177
HapII CCGG 2 cut(s) 135, 503
HhaI GCGC 1 cut(s) 466
Hin1I GRCGYC 1 cut(s) 464
Hin1II CATG 1 cut(s) 389
Hin6I GCGC 1 cut(s) 464
HinP1I GCGC 1 cut(s) 464
HincII GTYRAC 1 cut(s) 256
HindII GTYRAC 1 cut(s) 256
HinfI GANTC 6 cut(s) 44, 82, 114, 181, 443, 534
HpaI GTTAAC 1 cut(s) 256
HpaII CCGG 2 cut(s) 135, 503
Hpy166II GTNNAC 3 cut(s) 201, 256, 556
Hpy188I TCNGA 4 cut(s) 125, 246, 478, 539
Hpy188III TCNNGA 2 cut(s) 372, 503
Hpy8I GTNNAC 3 cut(s) 201, 256, 556
HpyCH4III ACNGT 1 cut(s) 553
HpyCH4V TGCA 2 cut(s) 347, 389
HpyF3I CTNAG 6 cut(s) 214, 245, 404, 477, 483, 536
Hsp92I GRCGYC 1 cut(s) 464
Hsp92II CATG 1 cut(s) 389
HspAI GCGC 1 cut(s) 464
KasI GGCGCC 1 cut(s) 463
Kpn2I TCCGGA 1 cut(s) 502
KspAI GTTAAC 1 cut(s) 256
Kzo9I GATC 1 cut(s) 525
LmnI GCTCC 1 cut(s) 223
Lsp1109I GCAGC 1 cut(s) 401
MaeI CTAG 1 cut(s) 50
MaeIII GTNAC 1 cut(s) 79
MalI GATC 1 cut(s) 527
MbiI CCGCTC 1 cut(s) 249
MboI GATC 1 cut(s) 525
MboII GAAGA 2 cut(s) 165, 374
MhlI GDGCHC 1 cut(s) 415
MluCI AATT 5 cut(s) 28, 240, 279, 375, 546
Mly113I GGCGCC 1 cut(s) 464
MlyI GAGTC 3 cut(s) 76, 175, 528
MnlI CCTC 6 cut(s) 114, 216, 266, 472, 480, 510
MroI TCCGGA 1 cut(s) 502
MseI TTAA 3 cut(s) 192, 255, 285
MspA1I CMGCKG 1 cut(s) 230
MspI CCGG 2 cut(s) 135, 503
MspR9I CCNGG 2 cut(s) 136, 355
MvaI CCWGG 1 cut(s) 355
NarI GGCGCC 1 cut(s) 464
NciI CCSGG 1 cut(s) 136
NdeII GATC 1 cut(s) 525
NlaIII CATG 1 cut(s) 389
NlaIV GGNNCC 1 cut(s) 465
NmuCI GTSAC 1 cut(s) 79
PfeI GAWTC 3 cut(s) 44, 114, 443
PkrI GCNGC 1 cut(s) 391
PleI GAGTC 3 cut(s) 76, 175, 528
PluTI GGCGCC 1 cut(s) 467
PpsI GAGTC 3 cut(s) 76, 175, 528
Psp6I CCWGG 1 cut(s) 353
PspGI CCWGG 1 cut(s) 353
PspN4I GGNNCC 1 cut(s) 465
PspPI GGNCC 1 cut(s) 233
PsrI GAACNNNNNNTAC 2 cut(s) 56, 88
PvuII CAGCTG 1 cut(s) 230
SaqAI TTAA 3 cut(s) 192, 255, 285
SatI GCNGC 1 cut(s) 390
Sau3AI GATC 1 cut(s) 525
Sau96I GGNCC 1 cut(s) 233
SchI GAGTC 3 cut(s) 76, 175, 528
ScrFI CCNGG 2 cut(s) 136, 355
SduI GDGCHC 1 cut(s) 415
SetI ASST 8 cut(s) 220, 232, 271, 277, 394, 402, 502, 512
SfoI GGCGCC 1 cut(s) 465
SinI GGWCC 1 cut(s) 233
SmlI CTYRAG 1 cut(s) 370
SmoI CTYRAG 1 cut(s) 370
Sse9I AATT 5 cut(s) 28, 240, 279, 375, 546
SsiI CCGC 1 cut(s) 249
SspDI GGCGCC 1 cut(s) 463
SspI AATATT 1 cut(s) 303
SspMI CTAG 1 cut(s) 50
StyD4I CCNGG 2 cut(s) 134, 353
StyI CCWWGG 1 cut(s) 90
TaaI ACNGT 1 cut(s) 553
TaqI TCGA 4 cut(s) 42, 117, 437, 494
TasI AATT 5 cut(s) 28, 240, 279, 375, 546
TfiI GAWTC 3 cut(s) 44, 114, 443
Tru1I TTAA 3 cut(s) 192, 255, 285
Tru9I TTAA 3 cut(s) 192, 255, 285
TseFI GTSAC 1 cut(s) 79
TseI GCWGC 1 cut(s) 389
Tsp45I GTSAC 1 cut(s) 79
TspDTI ATGAA 4 cut(s) 303, 374, 554, 558
VpaK11BI GGWCC 1 cut(s) 233
XapI RAATTY 1 cut(s) 279
XmiI GTMKAC 1 cut(s) 555
XspI CTAG 1 cut(s) 50
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.