Rh4DG262200

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0001879
Physical Location & Seq
Forward (+)
47998117 .. 47998675
559 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4DG262200.1

Sequence Viewer

Length: 477 bp
ATGGGTGGCATATTTATAGATGGAAAATTCATTCTTAGCATAATTTTTGCACTGTGGAGAATGTGTTTGGCAAAAGAATCTCTTGCTAATTTTGTGTTTGGGGATTCTCTGGTTGAAGTGGGGAACAACAACTACATTCCCTCACTCTCCAAGGCTGATTATCCTCCCAATGGAATCGATTTCGGAAAGCCTACCGGGCGATACACAAATGCAAGAACCGTCATTGACATTATAGGTCAGAGTGCTCTGGGTTTGAAGGAGTTCACTCCTCCTTACTTAGCTCCCACAACAGCTGGACCAGTAATTCTGAGGGGTGTTAACTATGCTTCAGGTGGAGGTGGAATTCTTAATGAAACAGGAAAAATATATGTAAGGGTTCTTCTGCTTTTCACTCGCTTTCGTTTGATCATATTAAATGTATACATTGAGCTAATGATATATAAATCATATGTAACTTTTGGTAGGTTGGTAGAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

158

Amino Acids

17.39

Weight (kDa)

9.24

Isoelectric Point (pI)

25.49

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 420
AcsI RAATTY 2 cut(s) 26, 342
AcuI CTGAAG 1 cut(s) 312
AfiI CCNNNNNNNGG 1 cut(s) 170
AgsI TTSAA 2 cut(s) 116, 256
AluBI AGCT 3 cut(s) 281, 293, 430
AluI AGCT 3 cut(s) 281, 293, 430
Alw21I GWGCWC 1 cut(s) 247
ApoI RAATTY 2 cut(s) 26, 342
Asp700I GAANNNNTTC 1 cut(s) 260
AspS9I GGNCC 1 cut(s) 296
AsuC2I CCSGG 1 cut(s) 196
AvaII GGWCC 1 cut(s) 296
BarI GAAGNNNNNNTAC 2 cut(s) 363, 395
Bbv12I GWGCWC 1 cut(s) 247
BccI CCATC 1 cut(s) 14
BclI TGATCA 1 cut(s) 405
BcnI CCSGG 1 cut(s) 196
BglI GCCNNNNNGGC 1 cut(s) 196
Bme1390I CCNGG 1 cut(s) 196
Bme18I GGWCC 1 cut(s) 296
BmgT120I GGNCC 1 cut(s) 296
BmrFI CCNGG 1 cut(s) 196
BpuMI CCSGG 1 cut(s) 196
Bsa29I ATCGAT 1 cut(s) 177
BsaJI CCNNGG 1 cut(s) 150
BsaXI ACNNNNNCTCC 2 cut(s) 49, 79
Bsc4I CCNNNNNNNGG 1 cut(s) 170
Bse1I ACTGG 1 cut(s) 299
BseCI ATCGAT 1 cut(s) 177
BseDI CCNNGG 1 cut(s) 150
BseLI CCNNNNNNNGG 1 cut(s) 170
BseMII CTCAG 1 cut(s) 299
BseNI ACTGG 1 cut(s) 299
BseRI GAGGAG 1 cut(s) 258
BshVI ATCGAT 1 cut(s) 177
BsiHKAI GWGCWC 1 cut(s) 247
BsiSI CCGG 1 cut(s) 195
BslI CCNNNNNNNGG 1 cut(s) 170
Bsp1286I GDGCHC 1 cut(s) 247
Bsp143I GATC 1 cut(s) 405
BspCNI CTCAG 1 cut(s) 300
BspDI ATCGAT 1 cut(s) 177
BsrI ACTGG 1 cut(s) 299
BssECI CCNNGG 1 cut(s) 150
BssMI GATC 1 cut(s) 405
BssNAI GTATAC 1 cut(s) 421
BssT1I CCWWGG 1 cut(s) 150
Bst1107I GTATAC 1 cut(s) 421
Bst4CI ACNGT 2 cut(s) 54, 220
BstDEI CTNAG 3 cut(s) 35, 277, 308
BstKTI GATC 1 cut(s) 408
BstMBI GATC 1 cut(s) 405
BstMWI GCNNNNNNNGC 1 cut(s) 196
BstSCI CCNGG 1 cut(s) 194
BstZ17I GTATAC 1 cut(s) 421
Bsu15I ATCGAT 1 cut(s) 177
BsuTUI ATCGAT 1 cut(s) 177
BtsIMutI CAGTG 1 cut(s) 50
Cfr13I GGNCC 1 cut(s) 296
ClaI ATCGAT 1 cut(s) 177
CviJI RGCY 5 cut(s) 155, 190, 281, 293, 430
CviKI_1 RGCY 5 cut(s) 155, 190, 281, 293, 430
DdeI CTNAG 3 cut(s) 35, 277, 308
DpnI GATC 1 cut(s) 407
DpnII GATC 1 cut(s) 405
Eco130I CCWWGG 1 cut(s) 150
Eco47I GGWCC 1 cut(s) 296
Eco57I CTGAAG 1 cut(s) 312
EcoRI GAATTC 1 cut(s) 342
EcoT14I CCWWGG 1 cut(s) 150
ErhI CCWWGG 1 cut(s) 150
FalI AAGNNNNNCTT 2 cut(s) 66, 98
FauNDI CATATG 1 cut(s) 448
FbaI TGATCA 1 cut(s) 405
FblI GTMKAC 1 cut(s) 420
HapII CCGG 1 cut(s) 195
HincII GTYRAC 1 cut(s) 319
HindII GTYRAC 1 cut(s) 319
HinfI GANTC 3 cut(s) 77, 104, 174
HpaI GTTAAC 1 cut(s) 319
HpaII CCGG 1 cut(s) 195
Hpy166II GTNNAC 3 cut(s) 264, 319, 421
Hpy188I TCNGA 3 cut(s) 185, 240, 309
Hpy8I GTNNAC 3 cut(s) 264, 319, 421
HpyAV CCTTC 1 cut(s) 250
HpyCH4III ACNGT 2 cut(s) 54, 220
HpyCH4V TGCA 2 cut(s) 50, 212
HpyF10VI GCNNNNNNNGC 1 cut(s) 196
HpyF3I CTNAG 3 cut(s) 35, 277, 308
Ksp22I TGATCA 1 cut(s) 405
KspAI GTTAAC 1 cut(s) 319
Kzo9I GATC 1 cut(s) 405
LmnI GCTCC 1 cut(s) 286
LpnPI CCDG 7 cut(s) 95, 208, 233, 279, 312, 315, 342
MaeIII GTNAC 1 cut(s) 451
MalI GATC 1 cut(s) 407
MboI GATC 1 cut(s) 405
MboII GAAGA 1 cut(s) 371
MhlI GDGCHC 1 cut(s) 247
MluCI AATT 5 cut(s) 26, 42, 88, 303, 342
MnlI CCTC 5 cut(s) 151, 174, 279, 303, 329
MroXI GAANNNNTTC 1 cut(s) 260
MseI TTAA 3 cut(s) 318, 348, 413
MspA1I CMGCKG 1 cut(s) 293
MspI CCGG 1 cut(s) 195
MspR9I CCNGG 1 cut(s) 196
MwoI GCNNNNNNNGC 1 cut(s) 196
NciI CCSGG 1 cut(s) 196
NdeI CATATG 1 cut(s) 448
NdeII GATC 1 cut(s) 405
PdmI GAANNNNTTC 1 cut(s) 260
PfeI GAWTC 3 cut(s) 77, 104, 174
PspPI GGNCC 1 cut(s) 296
PsrI GAACNNNNNNTAC 2 cut(s) 116, 148
PvuII CAGCTG 1 cut(s) 293
SaqAI TTAA 3 cut(s) 318, 348, 413
Sau3AI GATC 1 cut(s) 405
Sau96I GGNCC 1 cut(s) 296
ScrFI CCNGG 1 cut(s) 196
SduI GDGCHC 1 cut(s) 247
SetI ASST 7 cut(s) 238, 283, 295, 334, 340, 432, 467
SinI GGWCC 1 cut(s) 296
Sse9I AATT 5 cut(s) 26, 42, 88, 303, 342
StyD4I CCNGG 1 cut(s) 194
StyI CCWWGG 1 cut(s) 150
TaaI ACNGT 2 cut(s) 54, 220
TaqI TCGA 1 cut(s) 177
TasI AATT 5 cut(s) 26, 42, 88, 303, 342
TfiI GAWTC 3 cut(s) 77, 104, 174
Tru1I TTAA 3 cut(s) 318, 348, 413
Tru9I TTAA 3 cut(s) 318, 348, 413
TscAI CASTG 1 cut(s) 57
TspDTI ATGAA 2 cut(s) 19, 366
TspRI CASTG 1 cut(s) 57
VpaK11BI GGWCC 1 cut(s) 296
XapI RAATTY 2 cut(s) 26, 342
XmiI GTMKAC 1 cut(s) 420
XmnI GAANNNNTTC 1 cut(s) 260
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.