Rh4AG260100

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4A
OG0001879
Physical Location & Seq
Forward (+)
57614983 .. 57642580
27598 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4AG260100.1

Sequence Viewer

Length: 888 bp
ATGTGTTTGGCAAAAGAAACTCCTGCTAATTTTGTGTTTGGGGATTCTCTGGTTGAAGTGGGGAACAACAACTACATTCCCTCACTCTCCAAGGCTGATTATCCTCCCAATGGAATCGATTTCGGAAAGCCTACCGGGCGATACACAAATGCAAGAACCGTCATTGACATTATAGGTCAGAGTGCTCTGGGTTTGAAGGAGTTCACTCCTCCTTACTTAGCTCCCACAACAGCTGGACCAGTAATTCTGAGGGGTGTCAACTATGCTTCAGGTGGAGGTGGAATTCTTAATGAAACAGGAAAAATATATGTTGGTAGAATAAACATGGATGCACAGATAGATAATTTTGCAAATACCAGACAAGACATTATCTCAAGCATTGGTCTTCCTGCAGCAGTAGAGCTACTTAGAAGAGCTCTCTTCTCAGTAACAATCGGCTCAAACGATTTCCTCAGTAACTACTTGGTGCCAGTTATAACTGAGGTTAAGAGGAAGTTGATACCACCGGAAACTTTTGTTGGCATTTTGATTGCAAGATTCAGACTACAACTTACAAGACTGTACAATTTGGGTGCAAGGAAGATGGTTGTGATAAATTTAGGACCTATTGGGTGTATACCCTTTGAGAGGGAAGTAAATCTGGTAGCTGATCCAGATAAGTGTGCTGAATTTCCAAATCAATTAGCACGGTCATTCAATGCCAAGCTAAGGACCCTTGTCACCGAGCTAAACAAGAATCTTCCCGGGGCCAAATTTGTGTATGGAAATGTTCATAGTATTGTGGAAGACATCATTGAGAACTACATATCATATGGTGAGACTTGGTGCTTTTCCCCACAATCAGGAATTTTTTTGGCTTCCACTTCTCTGGAGCATCCGGAAGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

295

Amino Acids

32.27

Weight (kDa)

6.21

Isoelectric Point (pI)

33.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 11 - 263 1.6e-21 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 476
AccB1I GGYRCC 1 cut(s) 466
AccI GTMKAC 1 cut(s) 616
AccIII TCCGGA 1 cut(s) 877
AclWI GGATC 1 cut(s) 644
AcsI RAATTY 5 cut(s) 282, 595, 668, 752, 846
AcuI CTGAAG 1 cut(s) 252
AfaI GTAC 1 cut(s) 563
AfiI CCNNNNNNNGG 4 cut(s) 110, 627, 708, 842
AgsI TTSAA 3 cut(s) 56, 196, 697
AjuI GAANNNNNNNTTGG 2 cut(s) 501, 533
AluBI AGCT 7 cut(s) 221, 233, 403, 416, 647, 706, 727
AluI AGCT 7 cut(s) 221, 233, 403, 416, 647, 706, 727
Alw21I GWGCWC 2 cut(s) 187, 418
Alw26I GTCTC 1 cut(s) 812
AlwI GGATC 1 cut(s) 644
Ama87I CYCGRG 1 cut(s) 743
Aor13HI TCCGGA 1 cut(s) 877
AoxI GGCC 1 cut(s) 747
ApeKI GCWGC 1 cut(s) 392
ApoI RAATTY 5 cut(s) 282, 595, 668, 752, 846
Asp700I GAANNNNTTC 1 cut(s) 200
AspS9I GGNCC 4 cut(s) 236, 602, 711, 747
AsuC2I CCSGG 3 cut(s) 136, 744, 745
AsuHPI GGTGA 2 cut(s) 712, 827
AvaI CYCGRG 1 cut(s) 743
AvaII GGWCC 3 cut(s) 236, 602, 711
BanI GGYRCC 1 cut(s) 466
BanII GRGCYC 1 cut(s) 418
BbsI GAAGAC 2 cut(s) 377, 792
Bbv12I GWGCWC 2 cut(s) 187, 418
BbvI GCAGC 1 cut(s) 404
BccI CCATC 1 cut(s) 577
BcnI CCSGG 3 cut(s) 136, 744, 745
BcoDI GTCTC 1 cut(s) 812
BfmI CTRYAG 1 cut(s) 390
BglI GCCNNNNNGGC 1 cut(s) 136
BisI GCNGC 1 cut(s) 393
BlsI GCNGC 1 cut(s) 394
Bme1390I CCNGG 3 cut(s) 136, 744, 745
Bme18I GGWCC 3 cut(s) 236, 602, 711
BmeT110I CYCGRG 1 cut(s) 743
BmgT120I GGNCC 4 cut(s) 236, 602, 711, 747
BmiI GGNNCC 3 cut(s) 468, 713, 748
BmrFI CCNGG 3 cut(s) 136, 744, 745
BmsI GCATC 2 cut(s) 319, 883
BoxI GACNNNNGTC 1 cut(s) 716
BpiI GAAGAC 2 cut(s) 377, 792
Bpu10I CCTNAGC 1 cut(s) 707
BpuEI CTTGAG 1 cut(s) 358
BpuMI CCSGG 3 cut(s) 136, 744, 745
Bsa29I ATCGAT 1 cut(s) 117
BsaJI CCNNGG 3 cut(s) 90, 743, 744
BsaWI WCCGGW 2 cut(s) 505, 877
Bsc4I CCNNNNNNNGG 4 cut(s) 110, 627, 708, 842
Bse1I ACTGG 2 cut(s) 239, 470
BseAI TCCGGA 1 cut(s) 877
BseCI ATCGAT 1 cut(s) 117
BseDI CCNNGG 3 cut(s) 90, 743, 744
BseGI GGATG 2 cut(s) 334, 874
BseLI CCNNNNNNNGG 4 cut(s) 110, 627, 708, 842
BseMII CTCAG 4 cut(s) 239, 438, 466, 471
BseNI ACTGG 2 cut(s) 239, 470
BseRI GAGGAG 1 cut(s) 198
BseXI GCAGC 1 cut(s) 404
BshFI GGCC 1 cut(s) 749
BshNI GGYRCC 1 cut(s) 466
BshVI ATCGAT 1 cut(s) 117
BsiHKAI GWGCWC 2 cut(s) 187, 418
BsiHKCI CYCGRG 1 cut(s) 743
BsiSI CCGG 4 cut(s) 135, 506, 744, 878
BslI CCNNNNNNNGG 4 cut(s) 110, 627, 708, 842
BsmAI GTCTC 1 cut(s) 812
BsnI GGCC 1 cut(s) 749
BsoBI CYCGRG 1 cut(s) 743
Bsp1286I GDGCHC 2 cut(s) 187, 418
Bsp13I TCCGGA 1 cut(s) 877
Bsp1407I TGTACA 1 cut(s) 561
Bsp143I GATC 1 cut(s) 649
BspANI GGCC 1 cut(s) 749
BspCNI CTCAG 4 cut(s) 240, 437, 465, 472
BspDI ATCGAT 1 cut(s) 117
BspEI TCCGGA 1 cut(s) 877
BspLI GGNNCC 3 cut(s) 468, 713, 748
BspMAI CTGCAG 1 cut(s) 394
BspPI GGATC 1 cut(s) 644
BspQI GCTCTTC 1 cut(s) 406
BspT107I GGYRCC 1 cut(s) 466
BsrGI TGTACA 1 cut(s) 561
BsrI ACTGG 2 cut(s) 239, 470
BssECI CCNNGG 3 cut(s) 90, 743, 744
BssMI GATC 1 cut(s) 649
BssNAI GTATAC 1 cut(s) 617
BssT1I CCWWGG 1 cut(s) 90
Bst1107I GTATAC 1 cut(s) 617
Bst4CI ACNGT 3 cut(s) 160, 561, 690
Bst6I CTCTTC 2 cut(s) 406, 425
BstAUI TGTACA 1 cut(s) 561
BstDEI CTNAG 7 cut(s) 217, 248, 407, 424, 452, 480, 707
BstENI CCTNNNNNAGG 1 cut(s) 625
BstF5I GGATG 2 cut(s) 334, 874
BstKTI GATC 1 cut(s) 652
BstMAI GTCTC 1 cut(s) 812
BstMBI GATC 1 cut(s) 649
BstMWI GCNNNNNNNGC 1 cut(s) 136
BstPAI GACNNNNGTC 1 cut(s) 716
BstSCI CCNGG 3 cut(s) 134, 742, 743
BstSFI CTRYAG 1 cut(s) 390
BstV1I GCAGC 1 cut(s) 404
BstV2I GAAGAC 2 cut(s) 377, 792
BstXI CCANNNNNNTGG 1 cut(s) 868
BstZ17I GTATAC 1 cut(s) 617
Bsu15I ATCGAT 1 cut(s) 117
BsuRI GGCC 1 cut(s) 749
BsuTUI ATCGAT 1 cut(s) 117
BtsCI GGATG 2 cut(s) 334, 874
Cfr13I GGNCC 4 cut(s) 236, 602, 711, 747
Cfr9I CCCGGG 1 cut(s) 743
ClaI ATCGAT 1 cut(s) 117
Csp6I GTAC 1 cut(s) 562
CviAII CATG 1 cut(s) 325
CviQI GTAC 1 cut(s) 562
DdeI CTNAG 7 cut(s) 217, 248, 407, 424, 452, 480, 707
DpnI GATC 1 cut(s) 651
DpnII GATC 1 cut(s) 649
Eam1104I CTCTTC 2 cut(s) 406, 425
EarI CTCTTC 2 cut(s) 406, 425
Ecl136II GAGCTC 1 cut(s) 416
Eco130I CCWWGG 1 cut(s) 90
Eco24I GRGCYC 1 cut(s) 418
Eco47I GGWCC 3 cut(s) 236, 602, 711
Eco53kI GAGCTC 1 cut(s) 416
Eco57I CTGAAG 1 cut(s) 252
Eco88I CYCGRG 1 cut(s) 743
EcoICRI GAGCTC 1 cut(s) 416
EcoNI CCTNNNNNAGG 1 cut(s) 625
EcoO109I RGGNCCY 2 cut(s) 602, 711
EcoRI GAATTC 1 cut(s) 282
EcoT14I CCWWGG 1 cut(s) 90
EcoT38I GRGCYC 1 cut(s) 418
ErhI CCWWGG 1 cut(s) 90
FaeI CATG 1 cut(s) 328
FatI CATG 1 cut(s) 324
FauNDI CATATG 1 cut(s) 811
FblI GTMKAC 1 cut(s) 616
Fnu4HI GCNGC 1 cut(s) 393
FokI GGATG 2 cut(s) 341, 861
FriOI GRGCYC 1 cut(s) 418
Fsp4HI GCNGC 1 cut(s) 393
GluI GCNGC 1 cut(s) 393
HaeIII GGCC 1 cut(s) 749
HapII CCGG 4 cut(s) 135, 506, 744, 878
Hin1II CATG 1 cut(s) 328
HincII GTYRAC 1 cut(s) 259
HindII GTYRAC 1 cut(s) 259
HinfI GANTC 4 cut(s) 44, 114, 537, 736
HpaII CCGG 4 cut(s) 135, 506, 744, 878
HphI GGTGA 2 cut(s) 712, 827
Hpy166II GTNNAC 3 cut(s) 204, 259, 617
Hpy188I TCNGA 4 cut(s) 125, 180, 249, 542
Hpy188III TCNNGA 4 cut(s) 653, 843, 869, 878
Hpy8I GTNNAC 3 cut(s) 204, 259, 617
HpyAV CCTTC 1 cut(s) 190
HpyCH4III ACNGT 3 cut(s) 160, 561, 690
HpyCH4V TGCA 6 cut(s) 152, 332, 350, 392, 533, 575
HpyF10VI GCNNNNNNNGC 1 cut(s) 136
HpyF3I CTNAG 7 cut(s) 217, 248, 407, 424, 452, 480, 707
Hsp92II CATG 1 cut(s) 328
Kpn2I TCCGGA 1 cut(s) 877
Kzo9I GATC 1 cut(s) 649
LguI GCTCTTC 1 cut(s) 406
LmnI GCTCC 2 cut(s) 226, 871
Lsp1109I GCAGC 1 cut(s) 404
LweI GCATC 2 cut(s) 319, 883
MaeIII GTNAC 3 cut(s) 427, 455, 718
MalI GATC 1 cut(s) 651
MboI GATC 1 cut(s) 649
MboII GAAGA 6 cut(s) 377, 412, 423, 592, 731, 797
MhlI GDGCHC 2 cut(s) 187, 418
MnlI CCTC 9 cut(s) 91, 114, 219, 243, 269, 461, 475, 483, 621
MroI TCCGGA 1 cut(s) 877
MroXI GAANNNNTTC 1 cut(s) 200
MseI TTAA 2 cut(s) 288, 486
MspA1I CMGCKG 1 cut(s) 233
MspI CCGG 4 cut(s) 135, 506, 744, 878
MspR9I CCNGG 3 cut(s) 136, 744, 745
MwoI GCNNNNNNNGC 1 cut(s) 136
NciI CCSGG 3 cut(s) 136, 744, 745
NdeI CATATG 1 cut(s) 811
NdeII GATC 1 cut(s) 649
NlaIII CATG 1 cut(s) 328
NlaIV GGNNCC 3 cut(s) 468, 713, 748
NmuCI GTSAC 1 cut(s) 718
PciSI GCTCTTC 1 cut(s) 406
PcsI WCGNNNNNNNCGW 1 cut(s) 441
PdmI GAANNNNTTC 1 cut(s) 200
PfeI GAWTC 4 cut(s) 44, 114, 537, 736
PkrI GCNGC 1 cut(s) 394
PpuMI RGGWCCY 2 cut(s) 602, 711
PshAI GACNNNNGTC 1 cut(s) 716
PsiI TTATAA 1 cut(s) 476
Psp124BI GAGCTC 1 cut(s) 418
Psp5II RGGWCCY 2 cut(s) 602, 711
PspN4I GGNNCC 3 cut(s) 468, 713, 748
PspPI GGNCC 4 cut(s) 236, 602, 711, 747
PspPPI RGGWCCY 2 cut(s) 602, 711
PsrI GAACNNNNNNTAC 2 cut(s) 56, 88
PstI CTGCAG 1 cut(s) 394
PvuII CAGCTG 1 cut(s) 233
RsaI GTAC 1 cut(s) 563
RsaNI GTAC 1 cut(s) 562
SacI GAGCTC 1 cut(s) 418
SapI GCTCTTC 1 cut(s) 406
SaqAI TTAA 2 cut(s) 288, 486
SatI GCNGC 1 cut(s) 393
Sau3AI GATC 1 cut(s) 649
Sau96I GGNCC 4 cut(s) 236, 602, 711, 747
ScrFI CCNGG 3 cut(s) 136, 744, 745
SduI GDGCHC 2 cut(s) 187, 418
SfaNI GCATC 2 cut(s) 319, 883
SfcI CTRYAG 1 cut(s) 390
SinI GGWCC 3 cut(s) 236, 602, 711
SmaI CCCGGG 1 cut(s) 745
SmlI CTYRAG 1 cut(s) 373
SmoI CTYRAG 1 cut(s) 373
SstI GAGCTC 1 cut(s) 418
StyD4I CCNGG 3 cut(s) 134, 742, 743
StyI CCWWGG 1 cut(s) 90
TaaI ACNGT 3 cut(s) 160, 561, 690
TaqI TCGA 1 cut(s) 117
TatI WGTACW 1 cut(s) 561
TfiI GAWTC 4 cut(s) 44, 114, 537, 736
Tru1I TTAA 2 cut(s) 288, 486
Tru9I TTAA 2 cut(s) 288, 486
TseFI GTSAC 1 cut(s) 718
TseI GCWGC 1 cut(s) 392
Tsp45I GTSAC 1 cut(s) 718
TspDTI ATGAA 2 cut(s) 306, 761
TspMI CCCGGG 1 cut(s) 743
VpaK11BI GGWCC 3 cut(s) 236, 602, 711
XagI CCTNNNNNAGG 1 cut(s) 625
XapI RAATTY 5 cut(s) 282, 595, 668, 752, 846
XmaI CCCGGG 1 cut(s) 743
XmiI GTMKAC 1 cut(s) 616
XmnI GAANNNNTTC 1 cut(s) 200
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.