Basic Information

Type: gene

Biological Identity

Species

arabidopsis_thaliana

Chromosome

Orthogroup ID

OG0000462

Physical Location & Seq

Genomic Coordinates Reverse (-)

12990851 .. 12992585

Gene Structure 1735 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

AT4G25420.2

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1134 bp

ATGGCCGTAAGTTTCGTAACAACATCTCCTGAGGAAGAAGACAAACCGAAGCTAGGCCTTGGAAATATTCAAACTCCGTTAATCTTCAACCCTTCAATGCTTAACCTTCAAGCCAATATCCCAAACCAATTCATCTGGCCTGACGACGAAAAACCTTCCATCAACGTTCTCGAGCTTGATGTTCCTCTCATCGACCTTCAAAACCTTCTCTCTGATCCATCCTCCACTTTAGATGCTTCGAGACTGATCTCTGAGGCCTGTAAGAAGCACGGTTTCTTCCTCGTGGTCAATCACGGCATCAGCGAGGAGCTTATTTCAGACGCTCATGAATACACGAGCCGCTTCTTTGATATGCCTCTCTCCGAAAAACAGAGGGTTCTTAGAAAATCCGGTGAGAGTGTTGGCTACGCAAGCAGTTTCACCGGACGCTTCTCCACCAAGCTTCCATGGAAGGAGACCCTTTCTTTCCGGTTTTGCGACGACATGAGCCGCTCAAAATCCGTTCAAGATTACTTCTGCGATGCGTTGGGACATGGGTTTCAGCCATTTGGGAAGGTGTATCAAGAGTATTGTGAAGCAATGAGTTCTCTATCACTGAAGATCATGGAGCTTCTGGGGCTAAGTTTAGGCGTAAAACGGGACTACTTTAGAGAGTTTTTCGAAGAAAACGATTCAATAATGAGACTGAATTACTACCCTCCATGTATAAAACCAGATCTCACACTAGGAACAGGACCTCATTGTGATCCAACATCTCTTACCATCCTTCACCAAGACCATGTTAATGGCCTTCAAGTCTTTGTGGAAAATCAATGGCGCTCCATTCGTCCCAACCCCAAGGCCTTTGTGGTCAATATCGGCGATACTTTCATGGCTCTATCGAACGATAGATACAAGAGCTGCTTGCACCGGGCGGTGGTGAACAGCGAGAGCGAGAGGAAATCACTTGCATTCTTCTTGTGTCCGAAAAAAGACAGAGTAGTGACGCCACCGAGAGAGCTTTTGGACAGCATCACATCAAGAAGATACCCTGACTTCACATGGTCTATGTTCCTTGAGTTCACTCAGAAACATTATAGAGCAGACATGAACACTCTCCAAGCCTTTTCAGATTGGCTCACCAAACCCATCTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0000902 GO:0001101 GO:0003006 GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006082 GO:0006629 GO:0006720 GO:0006721 GO:0007154 GO:0007165 GO:0007275 GO:0008150 GO:0008152 GO:0008299 GO:0008610 GO:0009058 GO:0009314 GO:0009416 GO:0009628 GO:0009639 GO:0009648 GO:0009653 GO:0009685 GO:0009686 GO:0009719 GO:0009725 GO:0009739 GO:0009740 GO:0009755 GO:0009791 GO:0009826 GO:0009908 GO:0009987 GO:0010033 GO:0010228 GO:0010476 GO:0010817 GO:0016043 GO:0016049 GO:0016053 GO:0016101 GO:0016102 GO:0016114 GO:0016491 GO:0016705 GO:0016706 GO:0019752 GO:0022414 GO:0023052 GO:0032501 GO:0032502 GO:0032870 GO:0032989 GO:0033993 GO:0040007 GO:0042221 GO:0042445 GO:0042446 GO:0043436 GO:0044237 GO:0044238 GO:0044249 GO:0044255 GO:0044281 GO:0044283 GO:0044424 GO:0044464 GO:0045544 GO:0046394 GO:0048366 GO:0048367 GO:0048572 GO:0048573 GO:0048575 GO:0048589 GO:0048608 GO:0048731 GO:0048827 GO:0048856 GO:0048869 GO:0050789 GO:0050794 GO:0050896 GO:0051213 GO:0051716 GO:0055114 GO:0060560 GO:0061458 GO:0065007 GO:0065008 GO:0070887 GO:0071229 GO:0071310 GO:0071370 GO:0071396 GO:0071495 GO:0071704 GO:0071840 GO:0080167 GO:0090567 GO:0099402 GO:1901576 GO:1901700 GO:1901701

KEGG Pathways

Metabolic & Signaling

ko00904 ko01100 ko01110 map00904 map01100 map01110

Pfam Domains

Protein Families

2OG-FeII_Oxy DIOX_N

Protein Analysis

377

Amino Acids

43.22

Weight (kDa)

5.77

Isoelectric Point (pI)

44.22

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DIOX_N	PF14226	61 - 159	3.3e-26	non-haem dioxygenase in morphine synthesis N-terminal
2OG-FeII_Oxy	PF03171	224 - 293	2.3e-23	2OG-Fe(II) oxygenase superfamily

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0000462)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G25420 AT4G25420 AT5G51810 AT5G51810
fragaria_vesca	FvH4_7g12600 FvH4_7g12610 FvH4_7g12610 FvH4_7g28670
malus_domestica	MD00G1003600.v1.1 MD00G1123700.v1.1 MD01G1192100.v1.1 MD02G1198000.v1.1
prunus_persica	Prupe.2G150700_v2.0.a1 Prupe.2G286800_v2.0.a1
pyrus_communis	pycom02g16160
rosa_chinensis	RchiOBHm_Chr1g0351591 RchiOBHm_Chr1g0351601 RchiOBHm_Chr1g0351611 RchiOBHm_Chr1g0351621 RchiOBHm_Chr1g0351631 RchiOBHm_Chr1g0353771 RchiOBHm_Chr1g0353791 RchiOBHm_Chr1g0376161
rosa_laevigata	RLG00000026625 RLG00000028255 RLG00000028258 RLG00000028394 RLG00000028395 RLG00000028396 RLG00000028397 RLG00000028399
rosa_multiflora	Rmu_sc0001144.1_g000008 Rmu_sc0001144.1_g000010 Rmu_sc0001144.1_g000018 Rmu_sc0001144.1_g000029 Rmu_sc0002300.1_g000001 Rmu_sc0002300.1_g000013 Rmu_sc0005947.1_g000014 Rmu_sc0020670.1_g000001 Rmu_ssc0000116.1_g000048
rosa_roxburghii	Rroxscaffold_4G00282340 Rroxscaffold_4G00301610 Rroxscaffold_4G00301650 Rroxscaffold_4G00303660 Rroxscaffold_4G00303670 Rroxscaffold_4G00303690 Rroxscaffold_4G00303710
rosa_rugosa	Rorug01G0218900 Rorug01G0219000.1 Rorug01G0219100 Rorug01G0219300 Rorug01G0219400 Rorug01G0219500 Rorug01G0238200 Rorug01G0238400 Rorug01G0394200
rosa_samantha	Rh1AG233600 Rh1AG234000 Rh1AG234100 Rh1AG234200 Rh1AG250000 Rh1AG250100 Rh1AG405700 Rh1BG204300 Rh1BG204500 Rh1BG204700 Rh1BG204800 Rh1BG220300 Rh1BG220600 Rh1BG369400 Rh1CG218200 Rh1CG218600 Rh1CG218700 Rh1CG218800 Rh1CG232600 Rh1CG382100 Rh1DG231200 Rh1DG231500 Rh1DG231700 Rh1DG231800 Rh1DG247200 Rh1DG247500 Rh1DG399000
rosa_wichuraiana	Rw0G011370 Rw1G020360 Rw1G020370 Rw1G020380 Rw1G020390 Rw1G021810 Rw1G021830 Rw1G035990

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccBSI	CCGCTC	1 cut(s)	492
AciI	CCGC	3 cut(s)	340, 490, 914
AclI	AACGTT	1 cut(s)	165
AclWI	GGATC	2 cut(s)	209, 740
AcoI	YGGCCR	1 cut(s)	3
AcuI	CTGAAG	1 cut(s)	617
AcyI	GRCGYC	1 cut(s)	986
AfiI	CCNNNNNNNGG	2 cut(s)	53, 916
AgsI	TTSAA	8 cut(s)	71, 88, 96, 110, 200, 506, 675, 794
AluBI	AGCT	7 cut(s)	52, 175, 310, 442, 610, 900, 1000
AluI	AGCT	7 cut(s)	52, 175, 310, 442, 610, 900, 1000
Alw26I	GTCTC	3 cut(s)	235, 449, 676
AlwI	GGATC	2 cut(s)	209, 740
Ama87I	CYCGRG	1 cut(s)	170
AoxI	GGCC	6 cut(s)	3, 55, 137, 255, 787, 840
ApeKI	GCWGC	1 cut(s)	900
AspLEI	GCGC	1 cut(s)	819
AspS9I	GGNCC	1 cut(s)	734
AsuC2I	CCSGG	1 cut(s)	911
AsuHPI	GGTGA	5 cut(s)	404, 412, 761, 931, 1111
AsuII	TTCGAA	1 cut(s)	660
AvaI	CYCGRG	1 cut(s)	170
AvaII	GGWCC	1 cut(s)	734
AxyI	CCTNAGG	1 cut(s)	30
BauI	CACGAG	2 cut(s)	281, 334
BbsI	GAAGAC	1 cut(s)	45
BbvI	GCAGC	1 cut(s)	887
BccI	CCATC	3 cut(s)	167, 226, 770
BceAI	ACGGC	1 cut(s)	310
BcnI	CCSGG	1 cut(s)	911
BcoDI	GTCTC	3 cut(s)	235, 449, 676
BfaI	CTAG	2 cut(s)	53, 725
BfoI	RGCGCY	1 cut(s)	820
BglII	AGATCT	1 cut(s)	715
BisI	GCNGC	3 cut(s)	340, 490, 901
BlsI	GCNGC	3 cut(s)	341, 491, 902
Bme1390I	CCNGG	1 cut(s)	911
Bme18I	GGWCC	1 cut(s)	734
BmeT110I	CYCGRG	1 cut(s)	170
BmgT120I	GGNCC	1 cut(s)	734
BmrFI	CCNGG	1 cut(s)	911
BmsI	GCATC	4 cut(s)	223, 306, 511, 1020
BpiI	GAAGAC	1 cut(s)	45
Bpu14I	TTCGAA	1 cut(s)	660
BpuEI	CTTGAG	1 cut(s)	1076
BpuMI	CCSGG	1 cut(s)	911
BsaHI	GRCGYC	1 cut(s)	986
BsaI	GGTCTC	1 cut(s)	449
BsaJI	CCNNGG	3 cut(s)	58, 446, 837
BsaWI	WCCGGW	3 cut(s)	389, 422, 468
BsaXI	ACNNNNNCTCC	2 cut(s)	10, 40
Bsc4I	CCNNNNNNNGG	2 cut(s)	53, 916
Bse21I	CCTNAGG	1 cut(s)	30
Bse3DI	GCAATG	1 cut(s)	585
BseDI	CCNNGG	3 cut(s)	58, 446, 837
BseGI	GGATG	2 cut(s)	218, 762
BseLI	CCNNNNNNNGG	2 cut(s)	53, 916
BseMI	GCAATG	1 cut(s)	585
BseMII	CTCAG	3 cut(s)	21, 243, 1079
BseRI	GAGGAG	1 cut(s)	320
BseXI	GCAGC	1 cut(s)	887
BshFI	GGCC	6 cut(s)	5, 57, 139, 257, 789, 842
BsiHKCI	CYCGRG	1 cut(s)	170
BsiSI	CCGG	4 cut(s)	390, 423, 469, 910
BslFI	GGGAC	3 cut(s)	543, 653, 813
BslI	CCNNNNNNNGG	2 cut(s)	53, 916
BsmAI	GTCTC	3 cut(s)	235, 449, 676
BsmFI	GGGAC	3 cut(s)	543, 653, 813
BsmI	GAATGC	1 cut(s)	950
BsnI	GGCC	6 cut(s)	5, 57, 139, 257, 789, 842
Bso31I	GGTCTC	1 cut(s)	449
BsoBI	CYCGRG	1 cut(s)	170
Bsp119I	TTCGAA	1 cut(s)	660
Bsp143I	GATC	5 cut(s)	214, 246, 600, 715, 745
Bsp19I	CCATGG	1 cut(s)	446
BspACI	CCGC	3 cut(s)	340, 490, 914
BspANI	GGCC	6 cut(s)	5, 57, 139, 257, 789, 842
BspCNI	CTCAG	3 cut(s)	22, 244, 1078
BspHI	TCATGA	1 cut(s)	325
BspPI	GGATC	2 cut(s)	209, 740
BspT104I	TTCGAA	1 cut(s)	660
BspTNI	GGTCTC	1 cut(s)	449
BsrBI	CCGCTC	1 cut(s)	492
BsrDI	GCAATG	1 cut(s)	585
BssECI	CCNNGG	3 cut(s)	58, 446, 837
BssMI	GATC	5 cut(s)	214, 246, 600, 715, 745
BssNI	GRCGYC	1 cut(s)	986
BssSI	CACGAG	2 cut(s)	281, 334
BssT1I	CCWWGG	3 cut(s)	58, 446, 837
Bst2BI	CACGAG	2 cut(s)	281, 334
Bst4CI	ACNGT	1 cut(s)	272
BstACI	GRCGYC	1 cut(s)	986
BstBI	TTCGAA	1 cut(s)	660
BstC8I	GCNNGC	2 cut(s)	412, 905
BstDEI	CTNAG	5 cut(s)	30, 252, 380, 620, 1065
BstDSI	CCRYGG	1 cut(s)	446
BstF5I	GGATG	2 cut(s)	218, 762
BstH2I	RGCGCY	1 cut(s)	820
BstHHI	GCGC	1 cut(s)	819
BstKTI	GATC	5 cut(s)	217, 249, 603, 718, 748
BstMAI	GTCTC	3 cut(s)	235, 449, 676
BstMBI	GATC	5 cut(s)	214, 246, 600, 715, 745
BstMWI	GCNNNNNNNGC	2 cut(s)	411, 616
BstSCI	CCNGG	1 cut(s)	909
BstV1I	GCAGC	1 cut(s)	887
BstV2I	GAAGAC	1 cut(s)	45
BstX2I	RGATCY	1 cut(s)	715
BstXI	CCANNNNNNTGG	1 cut(s)	785
BstYI	RGATCY	1 cut(s)	715
Bsu36I	CCTNAGG	1 cut(s)	30
BsuRI	GGCC	6 cut(s)	5, 57, 139, 257, 789, 842
BtgI	CCRYGG	1 cut(s)	446
BtgZI	GCGATG	1 cut(s)	534
BtsCI	GGATG	2 cut(s)	218, 762
BtsIMutI	CAGTG	1 cut(s)	593
Cac8I	GCNNGC	2 cut(s)	412, 905
CciI	TCATGA	1 cut(s)	325
CfoI	GCGC	1 cut(s)	819
Cfr13I	GGNCC	1 cut(s)	734
CseI	GACGC	3 cut(s)	329, 435, 994
DdeI	CTNAG	5 cut(s)	30, 252, 380, 620, 1065
DpnI	GATC	5 cut(s)	216, 248, 602, 717, 747
DpnII	GATC	5 cut(s)	214, 246, 600, 715, 745
EaeI	YGGCCR	1 cut(s)	3
Eco130I	CCWWGG	3 cut(s)	58, 446, 837
Eco147I	AGGCCT	3 cut(s)	57, 257, 842
Eco31I	GGTCTC	1 cut(s)	449
Eco47I	GGWCC	1 cut(s)	734
Eco57I	CTGAAG	1 cut(s)	617
Eco81I	CCTNAGG	1 cut(s)	30
Eco88I	CYCGRG	1 cut(s)	170
EcoO109I	RGGNCCY	1 cut(s)	734
EcoT14I	CCWWGG	3 cut(s)	58, 446, 837
ErhI	CCWWGG	3 cut(s)	58, 446, 837
FalI	AAGNNNNNCTT	2 cut(s)	887, 919
FaqI	GGGAC	3 cut(s)	543, 653, 813
Fnu4HI	GCNGC	3 cut(s)	340, 490, 901
FokI	GGATG	2 cut(s)	205, 749
Fsp4HI	GCNGC	3 cut(s)	340, 490, 901
FspBI	CTAG	2 cut(s)	53, 725
GlaI	GCGC	1 cut(s)	818
GluI	GCNGC	3 cut(s)	340, 490, 901
HaeII	RGCGCY	1 cut(s)	820
HaeIII	GGCC	6 cut(s)	5, 57, 139, 257, 789, 842
HapII	CCGG	4 cut(s)	390, 423, 469, 910
HgaI	GACGC	3 cut(s)	329, 435, 994
HhaI	GCGC	1 cut(s)	819
Hin1I	GRCGYC	1 cut(s)	986
Hin6I	GCGC	1 cut(s)	817
HinP1I	GCGC	1 cut(s)	817
HindIII	AAGCTT	1 cut(s)	440
HinfI	GANTC	1 cut(s)	671
HpaII	CCGG	4 cut(s)	390, 423, 469, 910
HphI	GGTGA	5 cut(s)	404, 412, 761, 931, 1111
Hpy166II	GTNNAC	2 cut(s)	922, 1062
Hpy188I	TCNGA	7 cut(s)	214, 253, 319, 364, 966, 1068, 1111
Hpy188III	TCNNGA	7 cut(s)	29, 170, 240, 326, 506, 563, 1020
Hpy8I	GTNNAC	2 cut(s)	922, 1062
Hpy99I	CGWCG	2 cut(s)	149, 482
HpyAV	CCTTC	9 cut(s)	102, 116, 165, 206, 215, 445, 547, 776, 800
HpyCH4III	ACNGT	1 cut(s)	272
HpyCH4IV	ACGT	1 cut(s)	165
HpyCH4V	TGCA	2 cut(s)	907, 950
HpyF10VI	GCNNNNNNNGC	2 cut(s)	411, 616
HpyF3I	CTNAG	5 cut(s)	30, 252, 380, 620, 1065
HpySE526I	ACGT	1 cut(s)	165
Hsp92I	GRCGYC	1 cut(s)	986
HspAI	GCGC	1 cut(s)	817
Kzo9I	GATC	5 cut(s)	214, 246, 600, 715, 745
LmnI	GCTCC	3 cut(s)	307, 607, 824
Lsp1109I	GCAGC	1 cut(s)	887
LweI	GCATC	4 cut(s)	223, 306, 511, 1020
MaeI	CTAG	2 cut(s)	53, 725
MaeII	ACGT	1 cut(s)	165
MaeIII	GTNAC	2 cut(s)	16, 982
MalI	GATC	5 cut(s)	216, 248, 602, 717, 747
MbiI	CCGCTC	1 cut(s)	492
MboI	GATC	5 cut(s)	214, 246, 600, 715, 745
MboII	GAAGA	8 cut(s)	47, 50, 76, 268, 610, 674, 946, 1035
MflI	RGATCY	1 cut(s)	715
MluCI	AATT	2 cut(s)	128, 688
MmeI	TCCRAC	1 cut(s)	773
MseI	TTAA	3 cut(s)	80, 102, 783
MslI	CAYNNNNRTG	1 cut(s)	783
MspI	CCGG	4 cut(s)	390, 423, 469, 910
MspR9I	CCNGG	1 cut(s)	911
Mva1269I	GAATGC	1 cut(s)	950
MwoI	GCNNNNNNNGC	2 cut(s)	411, 616
NciI	CCSGG	1 cut(s)	911
NcoI	CCATGG	1 cut(s)	446
NdeII	GATC	5 cut(s)	214, 246, 600, 715, 745
NmuCI	GTSAC	1 cut(s)	982
NspV	TTCGAA	1 cut(s)	660
PaeR7I	CTCGAG	1 cut(s)	170
PagI	TCATGA	1 cut(s)	325
PceI	AGGCCT	3 cut(s)	57, 257, 842
PcsI	WCGNNNNNNNCGW	2 cut(s)	300, 666
PctI	GAATGC	1 cut(s)	950
PfeI	GAWTC	1 cut(s)	671
PkrI	GCNGC	3 cut(s)	341, 491, 902
PpuMI	RGGWCCY	1 cut(s)	734
Psp1406I	AACGTT	1 cut(s)	165
Psp5II	RGGWCCY	1 cut(s)	734
PspPI	GGNCC	1 cut(s)	734
PspPPI	RGGWCCY	1 cut(s)	734
PsrI	GAACNNNNNNTAC	2 cut(s)	875, 907
PsuI	RGATCY	1 cut(s)	715
RseI	CAYNNNNRTG	1 cut(s)	783
SaqAI	TTAA	3 cut(s)	80, 102, 783
SatI	GCNGC	3 cut(s)	340, 490, 901
Sau3AI	GATC	5 cut(s)	214, 246, 600, 715, 745
Sau96I	GGNCC	1 cut(s)	734
ScrFI	CCNGG	1 cut(s)	911
SfaNI	GCATC	4 cut(s)	223, 306, 511, 1020
Sfr274I	CTCGAG	1 cut(s)	170
SfuI	TTCGAA	1 cut(s)	660
SinI	GGWCC	1 cut(s)	734
SlaI	CTCGAG	1 cut(s)	170
SmiMI	CAYNNNNRTG	1 cut(s)	783
SmlI	CTYRAG	2 cut(s)	170, 1055
SmoI	CTYRAG	2 cut(s)	170, 1055
Sse9I	AATT	2 cut(s)	128, 688
SseBI	AGGCCT	3 cut(s)	57, 257, 842
SsiI	CCGC	3 cut(s)	340, 490, 914
SspI	AATATT	1 cut(s)	67
SspMI	CTAG	2 cut(s)	53, 725
StuI	AGGCCT	3 cut(s)	57, 257, 842
StyD4I	CCNGG	1 cut(s)	909
StyI	CCWWGG	3 cut(s)	58, 446, 837
TaaI	ACNGT	1 cut(s)	272
TaiI	ACGT	1 cut(s)	168
TaqI	TCGA	5 cut(s)	171, 192, 239, 660, 881
TasI	AATT	2 cut(s)	128, 688
TauI	GCSGC	2 cut(s)	342, 492
TfiI	GAWTC	1 cut(s)	671
Tru1I	TTAA	3 cut(s)	80, 102, 783
Tru9I	TTAA	3 cut(s)	80, 102, 783
TscAI	CASTG	1 cut(s)	600
TseFI	GTSAC	1 cut(s)	982
TseI	GCWGC	1 cut(s)	900
Tsp45I	GTSAC	1 cut(s)	982
TspDTI	ATGAA	4 cut(s)	121, 342, 859, 1103
TspGWI	ACGGA	2 cut(s)	66, 490
TspRI	CASTG	1 cut(s)	600
VpaK11BI	GGWCC	1 cut(s)	734
XcmI	CCANNNNNNNNNTGG	1 cut(s)	844
XhoI	CTCGAG	1 cut(s)	170
XspI	CTAG	2 cut(s)	53, 725

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.