Belongs to the iron ascorbate-dependent oxidoreductase family
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AasI | GACNNNNNNGTC | 1 cut(s) | 38 |
| Acc36I | ACCTGC | 1 cut(s) | 363 |
| AccI | GTMKAC | 1 cut(s) | 195 |
| AccIII | TCCGGA | 1 cut(s) | 250 |
| AciI | CCGC | 1 cut(s) | 583 |
| AclWI | GGATC | 5 cut(s) | 155, 242, 616, 686, 729 |
| AcoI | YGGCCR | 2 cut(s) | 80, 129 |
| AcsI | RAATTY | 1 cut(s) | 1004 |
| AcuI | CTGAAG | 1 cut(s) | 104 |
| AdeI | CACNNNGTG | 1 cut(s) | 410 |
| AfaI | GTAC | 4 cut(s) | 50, 506, 590, 974 |
| AfiI | CCNNNNNNNGG | 3 cut(s) | 718, 782, 1102 |
| AgsI | TTSAA | 2 cut(s) | 595, 740 |
| AjnI | CCWGG | 2 cut(s) | 97, 781 |
| AleI | CACNNNNGTG | 1 cut(s) | 701 |
| AluBI | AGCT | 9 cut(s) | 256, 269, 304, 363, 388, 556, 570, 846, 860 |
| AluI | AGCT | 9 cut(s) | 256, 269, 304, 363, 388, 556, 570, 846, 860 |
| Alw21I | GWGCWC | 3 cut(s) | 258, 440, 1040 |
| Alw26I | GTCTC | 2 cut(s) | 333, 395 |
| AlwI | GGATC | 5 cut(s) | 155, 242, 616, 686, 729 |
| Aor13HI | TCCGGA | 1 cut(s) | 250 |
| AoxI | GGCC | 4 cut(s) | 80, 129, 733, 989 |
| ApeKI | GCWGC | 3 cut(s) | 260, 360, 846 |
| ApoI | RAATTY | 1 cut(s) | 1004 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 42 |
| AspA2I | CCTAGG | 1 cut(s) | 965 |
| AspS9I | GGNCC | 3 cut(s) | 115, 680, 733 |
| AsuHPI | GGTGA | 4 cut(s) | 358, 488, 538, 818 |
| AvaII | GGWCC | 2 cut(s) | 115, 680 |
| AvrII | CCTAGG | 1 cut(s) | 965 |
| AxyI | CCTNAGG | 1 cut(s) | 626 |
| BanII | GRGCYC | 2 cut(s) | 258, 325 |
| BbsI | GAAGAC | 1 cut(s) | 280 |
| Bbv12I | GWGCWC | 3 cut(s) | 258, 440, 1040 |
| BbvI | GCAGC | 3 cut(s) | 247, 372, 833 |
| BccI | CCATC | 2 cut(s) | 140, 172 |
| BceAI | ACGGC | 1 cut(s) | 160 |
| BciT130I | CCWGG | 2 cut(s) | 99, 783 |
| BclI | TGATCA | 1 cut(s) | 948 |
| BcoDI | GTCTC | 2 cut(s) | 333, 395 |
| BfaI | CTAG | 3 cut(s) | 357, 966, 1001 |
| BfmI | CTRYAG | 1 cut(s) | 21 |
| BfuAI | ACCTGC | 1 cut(s) | 363 |
| BisI | GCNGC | 3 cut(s) | 261, 361, 847 |
| BlnI | CCTAGG | 1 cut(s) | 965 |
| BlsI | GCNGC | 3 cut(s) | 262, 362, 848 |
| BmcAI | AGTACT | 1 cut(s) | 590 |
| Bme1390I | CCNGG | 2 cut(s) | 99, 783 |
| Bme18I | GGWCC | 2 cut(s) | 115, 680 |
| BmgT120I | GGNCC | 3 cut(s) | 115, 680, 733 |
| BmiI | GGNNCC | 3 cut(s) | 103, 126, 734 |
| BmrFI | CCNGG | 2 cut(s) | 99, 783 |
| BmrI | ACTGGG | 2 cut(s) | 569, 680 |
| BmsI | GCATC | 1 cut(s) | 1036 |
| BmuI | ACTGGG | 2 cut(s) | 569, 680 |
| BpiI | GAAGAC | 1 cut(s) | 280 |
| Bpu10I | CCTNAGC | 1 cut(s) | 6 |
| BpuEI | CTTGAG | 1 cut(s) | 1054 |
| BsaJI | CCNNGG | 4 cut(s) | 238, 392, 965, 1096 |
| BsaWI | WCCGGW | 1 cut(s) | 250 |
| BsaXI | ACNNNNNCTCC | 4 cut(s) | 757, 787, 1098, 1128 |
| Bsc4I | CCNNNNNNNGG | 3 cut(s) | 718, 782, 1102 |
| Bse118I | RCCGGY | 1 cut(s) | 368 |
| Bse1I | ACTGG | 5 cut(s) | 433, 564, 675, 682, 1064 |
| Bse21I | CCTNAGG | 1 cut(s) | 626 |
| Bse3DI | GCAATG | 2 cut(s) | 547, 835 |
| BseAI | TCCGGA | 1 cut(s) | 250 |
| BseBI | CCWGG | 2 cut(s) | 99, 783 |
| BseDI | CCNNGG | 4 cut(s) | 238, 392, 965, 1096 |
| BseGI | GGATG | 1 cut(s) | 60 |
| BseLI | CCNNNNNNNGG | 3 cut(s) | 718, 782, 1102 |
| BseMI | GCAATG | 2 cut(s) | 547, 835 |
| BseMII | CTCAG | 4 cut(s) | 166, 338, 417, 557 |
| BseNI | ACTGG | 5 cut(s) | 433, 564, 675, 682, 1064 |
| BseXI | GCAGC | 3 cut(s) | 247, 372, 833 |
| BsgI | GTGCAG | 2 cut(s) | 210, 918 |
| BshFI | GGCC | 4 cut(s) | 82, 131, 735, 991 |
| BsiHKAI | GWGCWC | 3 cut(s) | 258, 440, 1040 |
| BsiSI | CCGG | 5 cut(s) | 83, 113, 128, 251, 369 |
| BslFI | GGGAC | 1 cut(s) | 576 |
| BslI | CCNNNNNNNGG | 3 cut(s) | 718, 782, 1102 |
| BsmAI | GTCTC | 2 cut(s) | 333, 395 |
| BsmFI | GGGAC | 1 cut(s) | 576 |
| BsnI | GGCC | 4 cut(s) | 82, 131, 735, 991 |
| Bsp1286I | GDGCHC | 4 cut(s) | 258, 325, 440, 1040 |
| Bsp13I | TCCGGA | 1 cut(s) | 250 |
| Bsp143I | GATC | 6 cut(s) | 160, 247, 621, 691, 721, 948 |
| Bsp19I | CCATGG | 1 cut(s) | 392 |
| BspACI | CCGC | 1 cut(s) | 583 |
| BspANI | GGCC | 4 cut(s) | 82, 131, 735, 991 |
| BspCNI | CTCAG | 4 cut(s) | 165, 337, 418, 558 |
| BspEI | TCCGGA | 1 cut(s) | 250 |
| BspHI | TCATGA | 1 cut(s) | 271 |
| BspLI | GGNNCC | 3 cut(s) | 103, 126, 734 |
| BspMI | ACCTGC | 1 cut(s) | 363 |
| BspPI | GGATC | 5 cut(s) | 155, 242, 616, 686, 729 |
| BsrDI | GCAATG | 2 cut(s) | 547, 835 |
| BsrFI | RCCGGY | 1 cut(s) | 368 |
| BsrI | ACTGG | 5 cut(s) | 433, 564, 675, 682, 1064 |
| BssAI | RCCGGY | 1 cut(s) | 368 |
| BssECI | CCNNGG | 4 cut(s) | 238, 392, 965, 1096 |
| BssMI | GATC | 6 cut(s) | 160, 247, 621, 691, 721, 948 |
| BssT1I | CCWWGG | 3 cut(s) | 392, 965, 1096 |
| Bst2UI | CCWGG | 2 cut(s) | 99, 783 |
| Bst4CI | ACNGT | 2 cut(s) | 344, 769 |
| BstC8I | GCNNGC | 3 cut(s) | 205, 258, 851 |
| BstDEI | CTNAG | 7 cut(s) | 6, 152, 324, 407, 426, 566, 626 |
| BstDSI | CCRYGG | 2 cut(s) | 238, 392 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 716 |
| BstF5I | GGATG | 1 cut(s) | 60 |
| BstKTI | GATC | 6 cut(s) | 163, 250, 624, 694, 724, 951 |
| BstMAI | GTCTC | 2 cut(s) | 333, 395 |
| BstMBI | GATC | 6 cut(s) | 160, 247, 621, 691, 721, 948 |
| BstMWI | GCNNNNNNNGC | 3 cut(s) | 266, 369, 997 |
| BstNI | CCWGG | 2 cut(s) | 99, 783 |
| BstNSI | RCATGY | 1 cut(s) | 207 |
| BstSCI | CCNGG | 2 cut(s) | 97, 781 |
| BstSFI | CTRYAG | 1 cut(s) | 21 |
| BstV1I | GCAGC | 3 cut(s) | 247, 372, 833 |
| BstV2I | GAAGAC | 1 cut(s) | 280 |
| Bsu36I | CCTNAGG | 1 cut(s) | 626 |
| BsuRI | GGCC | 4 cut(s) | 82, 131, 735, 991 |
| BtgI | CCRYGG | 2 cut(s) | 238, 392 |
| BtsCI | GGATG | 1 cut(s) | 60 |
| BtsIMutI | CAGTG | 5 cut(s) | 160, 440, 668, 765, 958 |
| BveI | ACCTGC | 1 cut(s) | 363 |
| Cac8I | GCNNGC | 3 cut(s) | 205, 258, 851 |
| CciI | TCATGA | 1 cut(s) | 271 |
| Cfr10I | RCCGGY | 1 cut(s) | 368 |
| Cfr13I | GGNCC | 3 cut(s) | 115, 680, 733 |
| Csp6I | GTAC | 4 cut(s) | 49, 505, 589, 973 |
| CviAII | CATG | 4 cut(s) | 204, 215, 272, 393 |
| CviQI | GTAC | 4 cut(s) | 49, 505, 589, 973 |
| DdeI | CTNAG | 7 cut(s) | 6, 152, 324, 407, 426, 566, 626 |
| DpnI | GATC | 6 cut(s) | 162, 249, 623, 693, 723, 950 |
| DpnII | GATC | 6 cut(s) | 160, 247, 621, 691, 721, 948 |
| DraIII | CACNNNGTG | 1 cut(s) | 410 |
| DrdI | GACNNNNNNGTC | 1 cut(s) | 38 |
| DseDI | GACNNNNNNGTC | 1 cut(s) | 38 |
| EaeI | YGGCCR | 2 cut(s) | 80, 129 |
| Ecl136II | GAGCTC | 1 cut(s) | 256 |
| Eco130I | CCWWGG | 3 cut(s) | 392, 965, 1096 |
| Eco24I | GRGCYC | 2 cut(s) | 258, 325 |
| Eco47I | GGWCC | 2 cut(s) | 115, 680 |
| Eco53kI | GAGCTC | 1 cut(s) | 256 |
| Eco57I | CTGAAG | 1 cut(s) | 104 |
| Eco81I | CCTNAGG | 1 cut(s) | 626 |
| EcoICRI | GAGCTC | 1 cut(s) | 256 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 716 |
| EcoO109I | RGGNCCY | 1 cut(s) | 733 |
| EcoRII | CCWGG | 2 cut(s) | 97, 781 |
| EcoT14I | CCWWGG | 3 cut(s) | 392, 965, 1096 |
| EcoT38I | GRGCYC | 2 cut(s) | 258, 325 |
| ErhI | CCWWGG | 3 cut(s) | 392, 965, 1096 |
| FaeI | CATG | 4 cut(s) | 207, 218, 275, 396 |
| FaiI | YATR | 6 cut(s) | 205, 216, 273, 394, 818, 855 |
| FalI | AAGNNNNNCTT | 4 cut(s) | 390, 422, 1094, 1126 |
| FaqI | GGGAC | 1 cut(s) | 576 |
| FatI | CATG | 4 cut(s) | 203, 214, 271, 392 |
| FauI | CCCGC | 1 cut(s) | 576 |
| FbaI | TGATCA | 1 cut(s) | 948 |
| FblI | GTMKAC | 1 cut(s) | 195 |
| Fnu4HI | GCNGC | 3 cut(s) | 261, 361, 847 |
| FokI | GGATG | 1 cut(s) | 47 |
| FriOI | GRGCYC | 2 cut(s) | 258, 325 |
| Fsp4HI | GCNGC | 3 cut(s) | 261, 361, 847 |
| FspBI | CTAG | 3 cut(s) | 357, 966, 1001 |
| GluI | GCNGC | 3 cut(s) | 261, 361, 847 |
| HaeIII | GGCC | 4 cut(s) | 82, 131, 735, 991 |
| HapII | CCGG | 5 cut(s) | 83, 113, 128, 251, 369 |
| Hin1II | CATG | 4 cut(s) | 207, 218, 275, 396 |
| HincII | GTYRAC | 1 cut(s) | 196 |
| HindII | GTYRAC | 1 cut(s) | 196 |
| HindIII | AAGCTT | 1 cut(s) | 386 |
| HinfI | GANTC | 4 cut(s) | 179, 184, 219, 1107 |
| HpaII | CCGG | 5 cut(s) | 83, 113, 128, 251, 369 |
| HphI | GGTGA | 4 cut(s) | 358, 488, 538, 818 |
| Hpy166II | GTNNAC | 4 cut(s) | 196, 235, 529, 1057 |
| Hpy188I | TCNGA | 6 cut(s) | 106, 310, 327, 427, 488, 520 |
| Hpy188III | TCNNGA | 4 cut(s) | 251, 272, 689, 719 |
| Hpy8I | GTNNAC | 4 cut(s) | 196, 235, 529, 1057 |
| Hpy99I | CGWCG | 3 cut(s) | 92, 200, 452 |
| HpyAV | CCTTC | 4 cut(s) | 75, 128, 325, 722 |
| HpyCH4III | ACNGT | 2 cut(s) | 344, 769 |
| HpyCH4V | TGCA | 7 cut(s) | 176, 191, 207, 828, 849, 881, 935 |
| HpyF10VI | GCNNNNNNNGC | 3 cut(s) | 266, 369, 997 |
| HpyF3I | CTNAG | 7 cut(s) | 6, 152, 324, 407, 426, 566, 626 |
| Hsp92II | CATG | 4 cut(s) | 207, 218, 275, 396 |
| Kpn2I | TCCGGA | 1 cut(s) | 250 |
| Ksp22I | TGATCA | 1 cut(s) | 948 |
| Kzo9I | GATC | 6 cut(s) | 160, 247, 621, 691, 721, 948 |
| LmnI | GCTCC | 4 cut(s) | 107, 130, 253, 553 |
| Lsp1109I | GCAGC | 3 cut(s) | 247, 372, 833 |
| LweI | GCATC | 1 cut(s) | 1036 |
| MaeI | CTAG | 3 cut(s) | 357, 966, 1001 |
| MaeIII | GTNAC | 3 cut(s) | 350, 703, 769 |
| MalI | GATC | 6 cut(s) | 162, 249, 623, 693, 723, 950 |
| MboI | GATC | 6 cut(s) | 160, 247, 621, 691, 721, 948 |
| MboII | GAAGA | 3 cut(s) | 214, 280, 491 |
| MhlI | GDGCHC | 4 cut(s) | 258, 325, 440, 1040 |
| MluCI | AATT | 1 cut(s) | 1004 |
| MlyI | GAGTC | 2 cut(s) | 188, 1116 |
| MmeI | TCCRAC | 3 cut(s) | 81, 129, 316 |
| MnlI | CCTC | 8 cut(s) | 149, 161, 193, 236, 291, 321, 693, 1066 |
| MroI | TCCGGA | 1 cut(s) | 250 |
| MroXI | GAANNNNTTC | 1 cut(s) | 42 |
| MseI | TTAA | 5 cut(s) | 378, 665, 1065, 1116, 1126 |
| MslI | CAYNNNNRTG | 1 cut(s) | 701 |
| MspI | CCGG | 5 cut(s) | 83, 113, 128, 251, 369 |
| MspR9I | CCNGG | 2 cut(s) | 99, 783 |
| MvaI | CCWGG | 2 cut(s) | 99, 783 |
| MwoI | GCNNNNNNNGC | 3 cut(s) | 266, 369, 997 |
| NcoI | CCATGG | 1 cut(s) | 392 |
| NdeII | GATC | 6 cut(s) | 160, 247, 621, 691, 721, 948 |
| NlaIII | CATG | 4 cut(s) | 207, 218, 275, 396 |
| NlaIV | GGNNCC | 3 cut(s) | 103, 126, 734 |
| NmuCI | GTSAC | 1 cut(s) | 703 |
| NspI | RCATGY | 1 cut(s) | 207 |
| OliI | CACNNNNGTG | 1 cut(s) | 701 |
| PaeI | GCATGC | 1 cut(s) | 207 |
| PagI | TCATGA | 1 cut(s) | 271 |
| PdmI | GAANNNNTTC | 1 cut(s) | 42 |
| PfeI | GAWTC | 2 cut(s) | 184, 219 |
| PkrI | GCNGC | 3 cut(s) | 262, 362, 848 |
| PleI | GAGTC | 2 cut(s) | 187, 1115 |
| PpsI | GAGTC | 2 cut(s) | 187, 1115 |
| Psp124BI | GAGCTC | 1 cut(s) | 258 |
| Psp6I | CCWGG | 2 cut(s) | 97, 781 |
| PspGI | CCWGG | 2 cut(s) | 97, 781 |
| PspN4I | GGNNCC | 3 cut(s) | 103, 126, 734 |
| PspPI | GGNCC | 3 cut(s) | 115, 680, 733 |
| RsaI | GTAC | 4 cut(s) | 50, 506, 590, 974 |
| RsaNI | GTAC | 4 cut(s) | 49, 505, 589, 973 |
| RseI | CAYNNNNRTG | 1 cut(s) | 701 |
| SacI | GAGCTC | 1 cut(s) | 258 |
| SalI | GTCGAC | 1 cut(s) | 194 |
| SaqAI | TTAA | 5 cut(s) | 378, 665, 1065, 1116, 1126 |
| SatI | GCNGC | 3 cut(s) | 261, 361, 847 |
| Sau3AI | GATC | 6 cut(s) | 160, 247, 621, 691, 721, 948 |
| Sau96I | GGNCC | 3 cut(s) | 115, 680, 733 |
| ScaI | AGTACT | 1 cut(s) | 590 |
| SchI | GAGTC | 2 cut(s) | 188, 1116 |
| ScrFI | CCNGG | 2 cut(s) | 99, 783 |
| SduI | GDGCHC | 4 cut(s) | 258, 325, 440, 1040 |
| SfaNI | GCATC | 1 cut(s) | 1036 |
| SfcI | CTRYAG | 1 cut(s) | 21 |
| SinI | GGWCC | 2 cut(s) | 115, 680 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 701 |
| SmlI | CTYRAG | 1 cut(s) | 1033 |
| SmoI | CTYRAG | 1 cut(s) | 1033 |
| SphI | GCATGC | 1 cut(s) | 207 |
| Sse9I | AATT | 1 cut(s) | 1004 |
| SsiI | CCGC | 1 cut(s) | 583 |
| SspI | AATATT | 1 cut(s) | 602 |
| SspMI | CTAG | 3 cut(s) | 357, 966, 1001 |
| SstI | GAGCTC | 1 cut(s) | 258 |
| StyD4I | CCNGG | 2 cut(s) | 97, 781 |
| StyI | CCWWGG | 3 cut(s) | 392, 965, 1096 |
| TaaI | ACNGT | 2 cut(s) | 344, 769 |
| TaqI | TCGA | 6 cut(s) | 30, 135, 182, 195, 450, 750 |
| TasI | AATT | 1 cut(s) | 1004 |
| TatI | WGTACW | 1 cut(s) | 588 |
| TfiI | GAWTC | 2 cut(s) | 184, 219 |
| Tru1I | TTAA | 5 cut(s) | 378, 665, 1065, 1116, 1126 |
| Tru9I | TTAA | 5 cut(s) | 378, 665, 1065, 1116, 1126 |
| TscAI | CASTG | 5 cut(s) | 160, 440, 675, 772, 958 |
| TseFI | GTSAC | 1 cut(s) | 703 |
| TseI | GCWGC | 3 cut(s) | 260, 360, 846 |
| Tsp45I | GTSAC | 1 cut(s) | 703 |
| TspDTI | ATGAA | 3 cut(s) | 64, 411, 704 |
| TspGWI | ACGGA | 1 cut(s) | 255 |
| TspRI | CASTG | 5 cut(s) | 160, 440, 675, 772, 958 |
| VpaK11BI | GGWCC | 2 cut(s) | 115, 680 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 716 |
| XapI | RAATTY | 1 cut(s) | 1004 |
| XceI | RCATGY | 1 cut(s) | 207 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 984 |
| XmaJI | CCTAGG | 1 cut(s) | 965 |
| XmiI | GTMKAC | 1 cut(s) | 195 |
| XmnI | GAANNNNTTC | 1 cut(s) | 42 |
| XspI | CTAG | 3 cut(s) | 357, 966, 1001 |
| ZrmI | AGTACT | 1 cut(s) | 590 |