Belongs to the iron ascorbate-dependent oxidoreductase family
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AasI | GACNNNNNNGTC | 1 cut(s) | 830 |
| Acc36I | ACCTGC | 1 cut(s) | 408 |
| AccII | CGCG | 1 cut(s) | 1110 |
| AciI | CCGC | 2 cut(s) | 771, 1108 |
| AclWI | GGATC | 2 cut(s) | 725, 768 |
| AcsI | RAATTY | 1 cut(s) | 1088 |
| AcuI | CTGAAG | 1 cut(s) | 66 |
| AcyI | GRCGYC | 1 cut(s) | 1131 |
| AfaI | GTAC | 3 cut(s) | 95, 323, 1058 |
| AfiI | CCNNNNNNNGG | 4 cut(s) | 484, 757, 1025, 1119 |
| AgsI | TTSAA | 3 cut(s) | 634, 673, 779 |
| AjnI | CCWGG | 3 cut(s) | 1049, 1070, 1125 |
| AloI | GAACNNNNNNTCC | 2 cut(s) | 560, 592 |
| AluBI | AGCT | 9 cut(s) | 163, 170, 314, 408, 595, 820, 864, 905, 930 |
| AluI | AGCT | 9 cut(s) | 163, 170, 314, 408, 595, 820, 864, 905, 930 |
| Alw21I | GWGCWC | 2 cut(s) | 390, 907 |
| AlwI | GGATC | 2 cut(s) | 725, 768 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 930 |
| AoxI | GGCC | 3 cut(s) | 125, 719, 1073 |
| ApeKI | GCWGC | 1 cut(s) | 930 |
| ApoI | RAATTY | 1 cut(s) | 1088 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 320, 352 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 655 |
| AspA2I | CCTAGG | 1 cut(s) | 610 |
| AspLEI | GCGC | 1 cut(s) | 1112 |
| AspS9I | GGNCC | 3 cut(s) | 126, 719, 1122 |
| AsuC2I | CCSGG | 1 cut(s) | 718 |
| AsuHPI | GGTGA | 5 cut(s) | 395, 527, 961, 1024, 1060 |
| AsuII | TTCGAA | 1 cut(s) | 645 |
| AvaII | GGWCC | 1 cut(s) | 1122 |
| AvrII | CCTAGG | 1 cut(s) | 610 |
| BanII | GRGCYC | 2 cut(s) | 370, 907 |
| BarI | GAAGNNNNNNTAC | 2 cut(s) | 1049, 1081 |
| BauI | CACGAG | 1 cut(s) | 134 |
| Bbv12I | GWGCWC | 2 cut(s) | 390, 907 |
| BbvI | GCAGC | 1 cut(s) | 917 |
| BccI | CCATC | 1 cut(s) | 505 |
| BciT130I | CCWGG | 3 cut(s) | 1051, 1072, 1127 |
| BciVI | GTATCC | 1 cut(s) | 199 |
| BcnI | CCSGG | 1 cut(s) | 718 |
| BfaI | CTAG | 3 cut(s) | 611, 710, 1085 |
| BfuAI | ACCTGC | 1 cut(s) | 408 |
| BfuI | GTATCC | 1 cut(s) | 199 |
| BisI | GCNGC | 1 cut(s) | 931 |
| BlnI | CCTAGG | 1 cut(s) | 610 |
| BlsI | GCNGC | 1 cut(s) | 932 |
| Bme1390I | CCNGG | 4 cut(s) | 718, 1051, 1072, 1127 |
| Bme18I | GGWCC | 1 cut(s) | 1122 |
| BmgT120I | GGNCC | 3 cut(s) | 126, 719, 1122 |
| BmiI | GGNNCC | 2 cut(s) | 715, 1124 |
| BmrFI | CCNGG | 4 cut(s) | 718, 1051, 1072, 1127 |
| BmsI | GCATC | 2 cut(s) | 67, 550 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 958, 990 |
| BpmI | CTGGAG | 1 cut(s) | 562 |
| Bpu10I | CCTNAGC | 1 cut(s) | 816 |
| Bpu14I | TTCGAA | 1 cut(s) | 645 |
| BpuMI | CCSGG | 1 cut(s) | 718 |
| BsaAI | YACGTR | 1 cut(s) | 210 |
| BsaHI | GRCGYC | 1 cut(s) | 1131 |
| BsaJI | CCNNGG | 6 cut(s) | 283, 437, 483, 610, 1049, 1125 |
| BsaXI | ACNNNNNCTCC | 6 cut(s) | 314, 344, 560, 590, 1121, 1151 |
| Bsc4I | CCNNNNNNNGG | 4 cut(s) | 484, 757, 1025, 1119 |
| Bse1I | ACTGG | 4 cut(s) | 402, 478, 579, 1148 |
| BseBI | CCWGG | 3 cut(s) | 1051, 1072, 1127 |
| BseDI | CCNNGG | 6 cut(s) | 283, 437, 483, 610, 1049, 1125 |
| BseGI | GGATG | 1 cut(s) | 737 |
| BseLI | CCNNNNNNNGG | 4 cut(s) | 484, 757, 1025, 1119 |
| BseMII | CTCAG | 3 cut(s) | 104, 213, 383 |
| BseNI | ACTGG | 4 cut(s) | 402, 478, 579, 1148 |
| BseRI | GAGGAG | 2 cut(s) | 162, 415 |
| BseXI | GCAGC | 1 cut(s) | 917 |
| Bsh1236I | CGCG | 1 cut(s) | 1110 |
| BshFI | GGCC | 3 cut(s) | 127, 721, 1075 |
| BsiHKAI | GWGCWC | 2 cut(s) | 390, 907 |
| BsiSI | CCGG | 1 cut(s) | 717 |
| BslFI | GGGAC | 1 cut(s) | 978 |
| BslI | CCNNNNNNNGG | 4 cut(s) | 484, 757, 1025, 1119 |
| BsmFI | GGGAC | 1 cut(s) | 978 |
| BsnI | GGCC | 3 cut(s) | 127, 721, 1075 |
| Bsp119I | TTCGAA | 1 cut(s) | 645 |
| Bsp1286I | GDGCHC | 3 cut(s) | 370, 390, 907 |
| Bsp143I | GATC | 3 cut(s) | 660, 730, 760 |
| Bsp19I | CCATGG | 2 cut(s) | 283, 437 |
| BspACI | CCGC | 2 cut(s) | 771, 1108 |
| BspANI | GGCC | 3 cut(s) | 127, 721, 1075 |
| BspCNI | CTCAG | 3 cut(s) | 103, 212, 382 |
| BspFNI | CGCG | 1 cut(s) | 1110 |
| BspHI | TCATGA | 2 cut(s) | 316, 663 |
| BspLI | GGNNCC | 2 cut(s) | 715, 1124 |
| BspMI | ACCTGC | 1 cut(s) | 408 |
| BspPI | GGATC | 2 cut(s) | 725, 768 |
| BspT104I | TTCGAA | 1 cut(s) | 645 |
| BsrI | ACTGG | 4 cut(s) | 402, 478, 579, 1148 |
| BssECI | CCNNGG | 6 cut(s) | 283, 437, 483, 610, 1049, 1125 |
| BssMI | GATC | 3 cut(s) | 660, 730, 760 |
| BssNI | GRCGYC | 1 cut(s) | 1131 |
| BssSI | CACGAG | 1 cut(s) | 134 |
| BssT1I | CCWWGG | 3 cut(s) | 283, 437, 610 |
| Bst2BI | CACGAG | 1 cut(s) | 134 |
| Bst2UI | CCWGG | 3 cut(s) | 1051, 1072, 1127 |
| Bst4CI | ACNGT | 3 cut(s) | 392, 728, 964 |
| BstACI | GRCGYC | 1 cut(s) | 1131 |
| BstBAI | YACGTR | 1 cut(s) | 210 |
| BstBI | TTCGAA | 1 cut(s) | 645 |
| BstC8I | GCNNGC | 2 cut(s) | 250, 935 |
| BstDEI | CTNAG | 4 cut(s) | 90, 199, 369, 816 |
| BstDSI | CCRYGG | 2 cut(s) | 283, 437 |
| BstEII | GGTNACC | 1 cut(s) | 1012 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 755 |
| BstF5I | GGATG | 1 cut(s) | 737 |
| BstFNI | CGCG | 1 cut(s) | 1110 |
| BstHHI | GCGC | 1 cut(s) | 1112 |
| BstKTI | GATC | 3 cut(s) | 663, 733, 763 |
| BstMBI | GATC | 3 cut(s) | 660, 730, 760 |
| BstMWI | GCNNNNNNNGC | 3 cut(s) | 86, 352, 414 |
| BstNI | CCWGG | 3 cut(s) | 1051, 1072, 1127 |
| BstNSI | RCATGY | 1 cut(s) | 252 |
| BstPI | GGTNACC | 1 cut(s) | 1012 |
| BstSCI | CCNGG | 4 cut(s) | 716, 1049, 1070, 1125 |
| BstUI | CGCG | 1 cut(s) | 1110 |
| BstV1I | GCAGC | 1 cut(s) | 917 |
| BsuI | GTATCC | 1 cut(s) | 199 |
| BsuRI | GGCC | 3 cut(s) | 127, 721, 1075 |
| BtgI | CCRYGG | 2 cut(s) | 283, 437 |
| BtsCI | GGATG | 1 cut(s) | 737 |
| BtsI | GCAGTG | 1 cut(s) | 951 |
| BtsIMutI | CAGTG | 4 cut(s) | 388, 586, 724, 951 |
| BveI | ACCTGC | 1 cut(s) | 408 |
| Cac8I | GCNNGC | 2 cut(s) | 250, 935 |
| CaiI | CAGNNNCTG | 1 cut(s) | 930 |
| CciI | TCATGA | 2 cut(s) | 316, 663 |
| CfoI | GCGC | 1 cut(s) | 1112 |
| Cfr13I | GGNCC | 3 cut(s) | 126, 719, 1122 |
| CseI | GACGC | 2 cut(s) | 833, 1139 |
| Csp6I | GTAC | 3 cut(s) | 94, 322, 1057 |
| CviAII | CATG | 8 cut(s) | 249, 260, 284, 317, 325, 438, 565, 664 |
| CviQI | GTAC | 3 cut(s) | 94, 322, 1057 |
| DdeI | CTNAG | 4 cut(s) | 90, 199, 369, 816 |
| DpnI | GATC | 3 cut(s) | 662, 732, 762 |
| DpnII | GATC | 3 cut(s) | 660, 730, 760 |
| DraI | TTTAAA | 1 cut(s) | 897 |
| DrdI | GACNNNNNNGTC | 1 cut(s) | 830 |
| DseDI | GACNNNNNNGTC | 1 cut(s) | 830 |
| EciI | GGCGGA | 1 cut(s) | 786 |
| Ecl136II | GAGCTC | 1 cut(s) | 905 |
| Eco130I | CCWWGG | 3 cut(s) | 283, 437, 610 |
| Eco24I | GRGCYC | 2 cut(s) | 370, 907 |
| Eco47I | GGWCC | 1 cut(s) | 1122 |
| Eco53kI | GAGCTC | 1 cut(s) | 905 |
| Eco57I | CTGAAG | 1 cut(s) | 66 |
| Eco91I | GGTNACC | 1 cut(s) | 1012 |
| EcoICRI | GAGCTC | 1 cut(s) | 905 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 755 |
| EcoO109I | RGGNCCY | 1 cut(s) | 1122 |
| EcoO65I | GGTNACC | 1 cut(s) | 1012 |
| EcoRII | CCWGG | 3 cut(s) | 1049, 1070, 1125 |
| EcoT14I | CCWWGG | 3 cut(s) | 283, 437, 610 |
| EcoT38I | GRGCYC | 2 cut(s) | 370, 907 |
| ErhI | CCWWGG | 3 cut(s) | 283, 437, 610 |
| FaeI | CATG | 8 cut(s) | 252, 263, 287, 320, 328, 441, 568, 667 |
| FaqI | GGGAC | 1 cut(s) | 978 |
| FatI | CATG | 8 cut(s) | 248, 259, 283, 316, 324, 437, 564, 663 |
| FauNDI | CATATG | 1 cut(s) | 122 |
| Fnu4HI | GCNGC | 1 cut(s) | 931 |
| FokI | GGATG | 1 cut(s) | 724 |
| FriOI | GRGCYC | 2 cut(s) | 370, 907 |
| Fsp4HI | GCNGC | 1 cut(s) | 931 |
| FspBI | CTAG | 3 cut(s) | 611, 710, 1085 |
| GlaI | GCGC | 1 cut(s) | 1111 |
| GluI | GCNGC | 1 cut(s) | 931 |
| GsuI | CTGGAG | 1 cut(s) | 562 |
| HaeIII | GGCC | 3 cut(s) | 127, 721, 1075 |
| HapII | CCGG | 1 cut(s) | 717 |
| HgaI | GACGC | 2 cut(s) | 833, 1139 |
| HhaI | GCGC | 1 cut(s) | 1112 |
| Hin1I | GRCGYC | 1 cut(s) | 1131 |
| Hin1II | CATG | 8 cut(s) | 252, 263, 287, 320, 328, 441, 568, 667 |
| Hin6I | GCGC | 1 cut(s) | 1110 |
| HinP1I | GCGC | 1 cut(s) | 1110 |
| HincII | GTYRAC | 3 cut(s) | 181, 280, 838 |
| HindII | GTYRAC | 3 cut(s) | 181, 280, 838 |
| HindIII | AAGCTT | 1 cut(s) | 818 |
| HinfI | GANTC | 4 cut(s) | 152, 329, 656, 1037 |
| HpaI | GTTAAC | 1 cut(s) | 280 |
| HpaII | CCGG | 1 cut(s) | 717 |
| HphI | GGTGA | 5 cut(s) | 395, 527, 961, 1024, 1060 |
| Hpy166II | GTNNAC | 6 cut(s) | 181, 280, 505, 544, 838, 952 |
| Hpy188I | TCNGA | 6 cut(s) | 151, 372, 461, 527, 602, 1141 |
| Hpy188III | TCNNGA | 4 cut(s) | 317, 535, 664, 758 |
| Hpy8I | GTNNAC | 6 cut(s) | 181, 280, 505, 544, 838, 952 |
| Hpy99I | CGWCG | 1 cut(s) | 836 |
| HpyAV | CCTTC | 6 cut(s) | 343, 532, 641, 761, 991, 1144 |
| HpyCH4III | ACNGT | 3 cut(s) | 392, 728, 964 |
| HpyCH4IV | ACGT | 2 cut(s) | 209, 507 |
| HpyCH4V | TGCA | 5 cut(s) | 227, 252, 298, 346, 937 |
| HpyF10VI | GCNNNNNNNGC | 3 cut(s) | 86, 352, 414 |
| HpyF3I | CTNAG | 4 cut(s) | 90, 199, 369, 816 |
| HpySE526I | ACGT | 2 cut(s) | 209, 507 |
| Hsp92I | GRCGYC | 1 cut(s) | 1131 |
| Hsp92II | CATG | 8 cut(s) | 252, 263, 287, 320, 328, 441, 568, 667 |
| HspAI | GCGC | 1 cut(s) | 1110 |
| KspAI | GTTAAC | 1 cut(s) | 280 |
| Kzo9I | GATC | 3 cut(s) | 660, 730, 760 |
| LmnI | GCTCC | 4 cut(s) | 152, 175, 592, 927 |
| Lsp1109I | GCAGC | 1 cut(s) | 917 |
| LweI | GCATC | 2 cut(s) | 67, 550 |
| MaeI | CTAG | 3 cut(s) | 611, 710, 1085 |
| MaeII | ACGT | 2 cut(s) | 209, 507 |
| MaeIII | GTNAC | 2 cut(s) | 808, 1012 |
| MalI | GATC | 3 cut(s) | 662, 732, 762 |
| MboI | GATC | 3 cut(s) | 660, 730, 760 |
| MboII | GAAGA | 4 cut(s) | 68, 530, 634, 976 |
| MhlI | GDGCHC | 3 cut(s) | 370, 390, 907 |
| MluCI | AATT | 3 cut(s) | 450, 878, 1088 |
| MlyI | GAGTC | 2 cut(s) | 161, 323 |
| MmeI | TCCRAC | 2 cut(s) | 598, 1164 |
| MroXI | GAANNNNTTC | 1 cut(s) | 655 |
| MseI | TTAA | 4 cut(s) | 221, 279, 743, 896 |
| MspI | CCGG | 1 cut(s) | 717 |
| MspR9I | CCNGG | 4 cut(s) | 718, 1051, 1072, 1127 |
| MvaI | CCWGG | 3 cut(s) | 1051, 1072, 1127 |
| MvnI | CGCG | 1 cut(s) | 1110 |
| MwoI | GCNNNNNNNGC | 3 cut(s) | 86, 352, 414 |
| NciI | CCSGG | 1 cut(s) | 718 |
| NcoI | CCATGG | 2 cut(s) | 283, 437 |
| NdeI | CATATG | 1 cut(s) | 122 |
| NdeII | GATC | 3 cut(s) | 660, 730, 760 |
| NlaIII | CATG | 8 cut(s) | 252, 263, 287, 320, 328, 441, 568, 667 |
| NlaIV | GGNNCC | 2 cut(s) | 715, 1124 |
| NmuCI | GTSAC | 1 cut(s) | 1012 |
| NspI | RCATGY | 1 cut(s) | 252 |
| NspV | TTCGAA | 1 cut(s) | 645 |
| PaeI | GCATGC | 1 cut(s) | 252 |
| PagI | TCATGA | 2 cut(s) | 316, 663 |
| PcsI | WCGNNNNNNNCGW | 2 cut(s) | 831, 1137 |
| PdmI | GAANNNNTTC | 1 cut(s) | 655 |
| PfeI | GAWTC | 2 cut(s) | 656, 1037 |
| PkrI | GCNGC | 1 cut(s) | 932 |
| PleI | GAGTC | 2 cut(s) | 160, 323 |
| PpsI | GAGTC | 2 cut(s) | 160, 323 |
| Ppu21I | YACGTR | 1 cut(s) | 210 |
| PpuMI | RGGWCCY | 1 cut(s) | 1122 |
| Psp124BI | GAGCTC | 1 cut(s) | 907 |
| Psp5II | RGGWCCY | 1 cut(s) | 1122 |
| Psp6I | CCWGG | 3 cut(s) | 1049, 1070, 1125 |
| PspEI | GGTNACC | 1 cut(s) | 1012 |
| PspGI | CCWGG | 3 cut(s) | 1049, 1070, 1125 |
| PspN4I | GGNNCC | 2 cut(s) | 715, 1124 |
| PspPI | GGNCC | 3 cut(s) | 126, 719, 1122 |
| PspPPI | RGGWCCY | 1 cut(s) | 1122 |
| PstNI | CAGNNNCTG | 1 cut(s) | 930 |
| RsaI | GTAC | 3 cut(s) | 95, 323, 1058 |
| RsaNI | GTAC | 3 cut(s) | 94, 322, 1057 |
| SacI | GAGCTC | 1 cut(s) | 907 |
| SaqAI | TTAA | 4 cut(s) | 221, 279, 743, 896 |
| SatI | GCNGC | 1 cut(s) | 931 |
| Sau3AI | GATC | 3 cut(s) | 660, 730, 760 |
| Sau96I | GGNCC | 3 cut(s) | 126, 719, 1122 |
| SchI | GAGTC | 2 cut(s) | 161, 323 |
| ScrFI | CCNGG | 4 cut(s) | 718, 1051, 1072, 1127 |
| SduI | GDGCHC | 3 cut(s) | 370, 390, 907 |
| SfaNI | GCATC | 2 cut(s) | 67, 550 |
| SfuI | TTCGAA | 1 cut(s) | 645 |
| SinI | GGWCC | 1 cut(s) | 1122 |
| SphI | GCATGC | 1 cut(s) | 252 |
| Sse9I | AATT | 3 cut(s) | 450, 878, 1088 |
| SsiI | CCGC | 2 cut(s) | 771, 1108 |
| SspI | AATATT | 1 cut(s) | 554 |
| SspMI | CTAG | 3 cut(s) | 611, 710, 1085 |
| SstI | GAGCTC | 1 cut(s) | 907 |
| StyD4I | CCNGG | 4 cut(s) | 716, 1049, 1070, 1125 |
| StyI | CCWWGG | 3 cut(s) | 283, 437, 610 |
| TaaI | ACNGT | 3 cut(s) | 392, 728, 964 |
| TaiI | ACGT | 2 cut(s) | 212, 510 |
| TaqI | TCGA | 4 cut(s) | 240, 645, 659, 1035 |
| TaqII | GACCGA | 1 cut(s) | 475 |
| TasI | AATT | 3 cut(s) | 450, 878, 1088 |
| TatI | WGTACW | 1 cut(s) | 321 |
| TfiI | GAWTC | 2 cut(s) | 656, 1037 |
| Tru1I | TTAA | 4 cut(s) | 221, 279, 743, 896 |
| Tru9I | TTAA | 4 cut(s) | 221, 279, 743, 896 |
| TscAI | CASTG | 4 cut(s) | 395, 586, 731, 951 |
| TseFI | GTSAC | 1 cut(s) | 1012 |
| TseI | GCWGC | 1 cut(s) | 930 |
| Tsp45I | GTSAC | 1 cut(s) | 1012 |
| TspDTI | ATGAA | 7 cut(s) | 47, 51, 69, 109, 581, 743, 863 |
| TspGWI | ACGGA | 1 cut(s) | 796 |
| TspRI | CASTG | 4 cut(s) | 395, 586, 731, 951 |
| VpaK11BI | GGWCC | 1 cut(s) | 1122 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 755 |
| XapI | RAATTY | 1 cut(s) | 1088 |
| XceI | RCATGY | 1 cut(s) | 252 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 586 |
| XmaJI | CCTAGG | 1 cut(s) | 610 |
| XmnI | GAANNNNTTC | 1 cut(s) | 655 |
| XspI | CTAG | 3 cut(s) | 611, 710, 1085 |