Basic Information

Type: gene

Biological Identity

Species

rosa_chinensis

Chromosome

Orthogroup ID

OG0000462

Physical Location & Seq

Genomic Coordinates Reverse (-)

44844033 .. 44848611

Gene Structure 4579 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

PRQ57735

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1137 bp

ATGTTAGCCATGCCAATGCCCCCTAACCATCCTTCATCACTAAACCAAACAAAGCATGAGAATGAACGAACTCCTTTGGTTTTCGATGCTCCAGTTCTTCAATACGAGTCCAACATACCACCCCAATTCATATGGCCAGACGACGATAAGCCATGCCCCGAGCGACCAGAGCTTCATGTTCCTCCCATTGACCTGAAAGGCTTTCTCAGCGGGGATGCTTTGATCGTCTCAGAAGCAATTCGTTCAGTCGATGAGGCATGCAAGAAACATGGATTCTTTCTTATTGTCAACCATGGAGTTGATTCGCAACTCATAGCAAAAGCTCATGAATACATGGACGCGTTCTTCAGCATGAAACTCGCAGAGAAGCAAAGGGCTCAGAGAAAGGTTGGTGACAGCTGCGGATATGCTAGTAGCTTCACTGGCAGGTTCTCCTCCAAACTTCCTTGGAAAGAAACACTTTCTTTCAGATACTGCACTGATCGCCAGTGCTCAACAGTTGTCGAGGACTATTTTGTGAATGTGATGGGTGAAGATTTCACAGAATTCGGGAAGGTTTACCAAGAATATGGTGAAGCCATGAACAATCTCAGTAATGGGATAATGGAGCTACTAGGACTGAGCCTAGGAGTTGGCCAAGAGTATTTCAAAGAATTCTTCGAAGGAAACGATTCCATAATGAGGTTGAACTACTATCCAATATGCCAAAAACCAGATTTAACCCTAGGAACTGGTCCTCACTGTGATCCCACATCCTTAACGATCCTGCATCAGGACCAAGTAGGTGGCCTTGAAGTGTTTGTAGATGAAAAGTGGCACGCCATTACTCCTATCCTTGATTCCTTCGTTATCAACGTTGGTGACACATTTATGGCTCTATCAAATGGGATTTACAAGAGCTGCTTGCACAGAGCAGTTGTAAACAACAAAATCGTAAGGAAATCACTTGCTTTCTTTCTTTGCCCAAACAAAGACAAAGTGGTGACCCCACCAACAAGTTTGGTCGACACTGAAACACCAAGGTTGTATCCTGACTTCACTTGGCCTAGTCTGCTAGAGTTTACACAGAAGCACTACAGGGCCGACACAAGAACCTTGGATGCTTTCTCCGACTGGATTTCACGGAAAAGCAACTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0000902 GO:0001101 GO:0003006 GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006082 GO:0006629 GO:0006720 GO:0006721 GO:0007154 GO:0007165 GO:0007275 GO:0008150 GO:0008152 GO:0008299 GO:0008610 GO:0009058 GO:0009314 GO:0009416 GO:0009628 GO:0009648 GO:0009653 GO:0009685 GO:0009686 GO:0009719 GO:0009725 GO:0009739 GO:0009740 GO:0009755 GO:0009791 GO:0009826 GO:0009908 GO:0009987 GO:0010033 GO:0010228 GO:0010476 GO:0010817 GO:0016043 GO:0016049 GO:0016053 GO:0016101 GO:0016102 GO:0016114 GO:0016491 GO:0016705 GO:0016706 GO:0019752 GO:0022414 GO:0023052 GO:0032501 GO:0032502 GO:0032870 GO:0032989 GO:0033993 GO:0040007 GO:0042221 GO:0042445 GO:0042446 GO:0043436 GO:0044237 GO:0044238 GO:0044249 GO:0044255 GO:0044281 GO:0044283 GO:0044424 GO:0044464 GO:0045544 GO:0046394 GO:0048366 GO:0048367 GO:0048572 GO:0048573 GO:0048575 GO:0048589 GO:0048608 GO:0048731 GO:0048827 GO:0048856 GO:0048869 GO:0050789 GO:0050794 GO:0050896 GO:0051213 GO:0051716 GO:0055114 GO:0060560 GO:0061458 GO:0065007 GO:0065008 GO:0070887 GO:0071229 GO:0071310 GO:0071370 GO:0071396 GO:0071495 GO:0071704 GO:0071840 GO:0090567 GO:0099402 GO:1901576 GO:1901700 GO:1901701

KEGG Pathways

Metabolic & Signaling

ko00904 ko01100 ko01110 map00904 map01100 map01110

Pfam Domains

Protein Families

2OG-FeII_Oxy DIOX_N

Protein Analysis

378

Amino Acids

42.89

Weight (kDa)

5.41

Isoelectric Point (pI)

29.85

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DIOX_N	PF14226	60 - 160	2e-22	non-haem dioxygenase in morphine synthesis N-terminal
2OG-FeII_Oxy	PF03171	224 - 322	7.8e-33	2OG-Fe(II) oxygenase superfamily

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0000462)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G25420 AT4G25420 AT5G51810 AT5G51810
fragaria_vesca	FvH4_7g12600 FvH4_7g12610 FvH4_7g12610 FvH4_7g28670
malus_domestica	MD00G1003600.v1.1 MD00G1123700.v1.1 MD01G1192100.v1.1 MD02G1198000.v1.1
prunus_persica	Prupe.2G150700_v2.0.a1 Prupe.2G286800_v2.0.a1
pyrus_communis	pycom02g16160
rosa_chinensis	RchiOBHm_Chr1g0351591 RchiOBHm_Chr1g0351601 RchiOBHm_Chr1g0351611 RchiOBHm_Chr1g0351621 RchiOBHm_Chr1g0351631 RchiOBHm_Chr1g0353771 RchiOBHm_Chr1g0353791 RchiOBHm_Chr1g0376161
rosa_laevigata	RLG00000026625 RLG00000028255 RLG00000028258 RLG00000028394 RLG00000028395 RLG00000028396 RLG00000028397 RLG00000028399
rosa_multiflora	Rmu_sc0001144.1_g000008 Rmu_sc0001144.1_g000010 Rmu_sc0001144.1_g000018 Rmu_sc0001144.1_g000029 Rmu_sc0002300.1_g000001 Rmu_sc0002300.1_g000013 Rmu_sc0005947.1_g000014 Rmu_sc0020670.1_g000001 Rmu_ssc0000116.1_g000048
rosa_roxburghii	Rroxscaffold_4G00282340 Rroxscaffold_4G00301610 Rroxscaffold_4G00301650 Rroxscaffold_4G00303660 Rroxscaffold_4G00303670 Rroxscaffold_4G00303690 Rroxscaffold_4G00303710
rosa_rugosa	Rorug01G0218900 Rorug01G0219000.1 Rorug01G0219100 Rorug01G0219300 Rorug01G0219400 Rorug01G0219500 Rorug01G0238200 Rorug01G0238400 Rorug01G0394200
rosa_samantha	Rh1AG233600 Rh1AG234000 Rh1AG234100 Rh1AG234200 Rh1AG250000 Rh1AG250100 Rh1AG405700 Rh1BG204300 Rh1BG204500 Rh1BG204700 Rh1BG204800 Rh1BG220300 Rh1BG220600 Rh1BG369400 Rh1CG218200 Rh1CG218600 Rh1CG218700 Rh1CG218800 Rh1CG232600 Rh1CG382100 Rh1DG231200 Rh1DG231500 Rh1DG231700 Rh1DG231800 Rh1DG247200 Rh1DG247500 Rh1DG399000
rosa_wichuraiana	Rw0G011370 Rw1G020360 Rw1G020370 Rw1G020380 Rw1G020390 Rw1G021810 Rw1G021830 Rw1G035990

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	417
AccI	GTMKAC	1 cut(s)	1005
AccII	CGCG	1 cut(s)	341
AciI	CCGC	2 cut(s)	210, 402
AclI	AACGTT	1 cut(s)	855
AclWI	GGATC	2 cut(s)	740, 757
AcoI	YGGCCR	2 cut(s)	134, 634
AcsI	RAATTY	2 cut(s)	545, 653
AcuI	CTGAAG	1 cut(s)	331
AfiI	CCNNNNNNNGG	2 cut(s)	681, 772
AflIII	ACRYGT	1 cut(s)	339
AgsI	TTSAA	4 cut(s)	101, 649, 688, 794
AluBI	AGCT	6 cut(s)	172, 323, 399, 417, 610, 900
AluI	AGCT	6 cut(s)	172, 323, 399, 417, 610, 900
Alw21I	GWGCWC	1 cut(s)	494
Alw26I	GTCTC	1 cut(s)	232
AlwI	GGATC	2 cut(s)	740, 757
AlwNI	CAGNNNCTG	1 cut(s)	474
Ama87I	CYCGRG	1 cut(s)	158
AoxI	GGCC	5 cut(s)	134, 634, 787, 1043, 1080
ApeKI	GCWGC	2 cut(s)	399, 900
ApoI	RAATTY	2 cut(s)	545, 653
Asp700I	GAANNNNTTC	2 cut(s)	237, 670
AspA2I	CCTAGG	2 cut(s)	625, 724
AspS9I	GGNCC	3 cut(s)	734, 775, 1080
AsuHPI	GGTGA	5 cut(s)	404, 542, 584, 872, 994
AsuII	TTCGAA	1 cut(s)	660
AvaI	CYCGRG	1 cut(s)	158
AvaII	GGWCC	2 cut(s)	734, 775
AvrII	CCTAGG	2 cut(s)	625, 724
BalI	TGGCCA	2 cut(s)	136, 636
BanII	GRGCYC	1 cut(s)	379
BarI	GAAGNNNNNNTAC	2 cut(s)	1019, 1051
Bbv12I	GWGCWC	1 cut(s)	494
BbvI	GCAGC	2 cut(s)	386, 887
BccI	CCATC	2 cut(s)	36, 520
BcgI	CGANNNNNNTGC	2 cut(s)	340, 374
BciVI	GTATCC	1 cut(s)	1038
BcoDI	GTCTC	1 cut(s)	232
BfaI	CTAG	6 cut(s)	411, 614, 626, 725, 1047, 1055
BfmI	CTRYAG	1 cut(s)	1075
BfuAI	ACCTGC	1 cut(s)	417
BfuI	GTATCC	1 cut(s)	1038
BisI	GCNGC	2 cut(s)	400, 901
BlnI	CCTAGG	2 cut(s)	625, 724
BlsI	GCNGC	2 cut(s)	401, 902
Bme18I	GGWCC	2 cut(s)	734, 775
BmeT110I	CYCGRG	1 cut(s)	158
BmgT120I	GGNCC	3 cut(s)	734, 775, 1080
BmsI	GCATC	4 cut(s)	76, 205, 778, 1090
BpmI	CTGGAG	1 cut(s)	75
Bpu14I	TTCGAA	1 cut(s)	660
BsaJI	CCNNGG	6 cut(s)	292, 446, 625, 724, 1019, 1095
Bsc4I	CCNNNNNNNGG	2 cut(s)	681, 772
Bse1I	ACTGG	5 cut(s)	92, 427, 487, 736, 1118
BseDI	CCNNGG	6 cut(s)	292, 446, 625, 724, 1019, 1095
BseGI	GGATG	4 cut(s)	28, 220, 752, 1105
BseLI	CCNNNNNNNGG	2 cut(s)	681, 772
BseMII	CTCAG	5 cut(s)	220, 243, 392, 604, 611
BseNI	ACTGG	5 cut(s)	92, 427, 487, 736, 1118
BseRI	GAGGAG	1 cut(s)	424
BseXI	GCAGC	2 cut(s)	386, 887
BsgI	GTGCAG	1 cut(s)	460
Bsh1236I	CGCG	1 cut(s)	341
BshFI	GGCC	5 cut(s)	136, 636, 789, 1045, 1082
BsiHKAI	GWGCWC	1 cut(s)	494
BsiHKCI	CYCGRG	1 cut(s)	158
BslI	CCNNNNNNNGG	2 cut(s)	681, 772
BsmAI	GTCTC	1 cut(s)	232
BsmBI	CGTCTC	1 cut(s)	232
BsnI	GGCC	5 cut(s)	136, 636, 789, 1045, 1082
BsoBI	CYCGRG	1 cut(s)	158
Bsp119I	TTCGAA	1 cut(s)	660
Bsp1286I	GDGCHC	2 cut(s)	379, 494
Bsp143I	GATC	4 cut(s)	222, 481, 745, 762
Bsp19I	CCATGG	1 cut(s)	292
BspACI	CCGC	2 cut(s)	210, 402
BspANI	GGCC	5 cut(s)	136, 636, 789, 1045, 1082
BspCNI	CTCAG	5 cut(s)	219, 242, 391, 603, 612
BspFNI	CGCG	1 cut(s)	341
BspHI	TCATGA	1 cut(s)	325
BspMI	ACCTGC	1 cut(s)	417
BspPI	GGATC	2 cut(s)	740, 757
BspT104I	TTCGAA	1 cut(s)	660
BsrI	ACTGG	5 cut(s)	92, 427, 487, 736, 1118
BssECI	CCNNGG	6 cut(s)	292, 446, 625, 724, 1019, 1095
BssMI	GATC	4 cut(s)	222, 481, 745, 762
BssT1I	CCWWGG	6 cut(s)	292, 446, 625, 724, 1019, 1095
Bst4CI	ACNGT	2 cut(s)	499, 743
BstBI	TTCGAA	1 cut(s)	660
BstC8I	GCNNGC	3 cut(s)	259, 819, 905
BstDEI	CTNAG	5 cut(s)	206, 229, 378, 590, 620
BstDSI	CCRYGG	1 cut(s)	292
BstEII	GGTNACC	1 cut(s)	982
BstENI	CCTNNNNNAGG	1 cut(s)	770
BstF5I	GGATG	4 cut(s)	28, 220, 752, 1105
BstFNI	CGCG	1 cut(s)	341
BstKTI	GATC	4 cut(s)	225, 484, 748, 765
BstMAI	GTCTC	1 cut(s)	232
BstMBI	GATC	4 cut(s)	222, 481, 745, 762
BstMWI	GCNNNNNNNGC	5 cut(s)	169, 207, 423, 483, 1051
BstNSI	RCATGY	1 cut(s)	261
BstPI	GGTNACC	1 cut(s)	982
BstSFI	CTRYAG	1 cut(s)	1075
BstUI	CGCG	1 cut(s)	341
BstV1I	GCAGC	2 cut(s)	386, 887
BstXI	CCANNNNNNTGG	2 cut(s)	569, 785
BsuI	GTATCC	1 cut(s)	1038
BsuRI	GGCC	5 cut(s)	136, 636, 789, 1045, 1082
BtgI	CCRYGG	1 cut(s)	292
BtsCI	GGATG	4 cut(s)	28, 220, 752, 1105
BtsIMutI	CAGTG	5 cut(s)	420, 477, 494, 739, 1008
BveI	ACCTGC	1 cut(s)	417
Cac8I	GCNNGC	3 cut(s)	259, 819, 905
CaiI	CAGNNNCTG	1 cut(s)	474
CciI	TCATGA	1 cut(s)	325
Cfr13I	GGNCC	3 cut(s)	734, 775, 1080
CseI	GACGC	1 cut(s)	347
DdeI	CTNAG	5 cut(s)	206, 229, 378, 590, 620
DpnI	GATC	4 cut(s)	224, 483, 747, 764
DpnII	GATC	4 cut(s)	222, 481, 745, 762
EaeI	YGGCCR	2 cut(s)	134, 634
Eco130I	CCWWGG	6 cut(s)	292, 446, 625, 724, 1019, 1095
Eco24I	GRGCYC	1 cut(s)	379
Eco47I	GGWCC	2 cut(s)	734, 775
Eco57I	CTGAAG	1 cut(s)	331
Eco88I	CYCGRG	1 cut(s)	158
Eco91I	GGTNACC	1 cut(s)	982
EcoNI	CCTNNNNNAGG	1 cut(s)	770
EcoO65I	GGTNACC	1 cut(s)	982
EcoRI	GAATTC	2 cut(s)	545, 653
EcoT14I	CCWWGG	6 cut(s)	292, 446, 625, 724, 1019, 1095
EcoT38I	GRGCYC	1 cut(s)	379
ErhI	CCWWGG	6 cut(s)	292, 446, 625, 724, 1019, 1095
Esp3I	CGTCTC	1 cut(s)	232
FalI	AAGNNNNNCTT	4 cut(s)	444, 476, 887, 919
FauI	CCCGC	1 cut(s)	203
FauNDI	CATATG	1 cut(s)	131
FblI	GTMKAC	1 cut(s)	1005
Fnu4HI	GCNGC	2 cut(s)	400, 901
FokI	GGATG	4 cut(s)	15, 227, 739, 1112
FriOI	GRGCYC	1 cut(s)	379
Fsp4HI	GCNGC	2 cut(s)	400, 901
FspBI	CTAG	6 cut(s)	411, 614, 626, 725, 1047, 1055
GluI	GCNGC	2 cut(s)	400, 901
GsuI	CTGGAG	1 cut(s)	75
HaeIII	GGCC	5 cut(s)	136, 636, 789, 1045, 1082
HgaI	GACGC	1 cut(s)	347
HincII	GTYRAC	2 cut(s)	289, 1006
HindII	GTYRAC	2 cut(s)	289, 1006
HinfI	GANTC	5 cut(s)	107, 273, 302, 671, 839
HphI	GGTGA	5 cut(s)	404, 542, 584, 872, 994
Hpy166II	GTNNAC	5 cut(s)	289, 559, 922, 1006, 1062
Hpy188I	TCNGA	4 cut(s)	232, 381, 470, 1111
Hpy188III	TCNNGA	4 cut(s)	326, 550, 773, 1031
Hpy8I	GTNNAC	5 cut(s)	289, 559, 922, 1006, 1062
Hpy99I	CGWCG	1 cut(s)	146
HpyAV	CCTTC	4 cut(s)	42, 547, 656, 853
HpyCH4III	ACNGT	2 cut(s)	499, 743
HpyCH4IV	ACGT	1 cut(s)	855
HpyCH4V	TGCA	4 cut(s)	261, 477, 769, 907
HpyF10VI	GCNNNNNNNGC	5 cut(s)	169, 207, 423, 483, 1051
HpyF3I	CTNAG	5 cut(s)	206, 229, 378, 590, 620
HpySE526I	ACGT	1 cut(s)	855
Kzo9I	GATC	4 cut(s)	222, 481, 745, 762
LmnI	GCTCC	2 cut(s)	94, 607
Lsp1109I	GCAGC	2 cut(s)	386, 887
LweI	GCATC	4 cut(s)	76, 205, 778, 1090
MaeI	CTAG	6 cut(s)	411, 614, 626, 725, 1047, 1055
MaeII	ACGT	1 cut(s)	855
MaeIII	GTNAC	3 cut(s)	392, 860, 982
MalI	GATC	4 cut(s)	224, 483, 747, 764
MboI	GATC	4 cut(s)	222, 481, 745, 762
MboII	GAAGA	4 cut(s)	89, 337, 545, 649
MhlI	GDGCHC	2 cut(s)	379, 494
MlsI	TGGCCA	2 cut(s)	136, 636
MluCI	AATT	4 cut(s)	125, 237, 545, 653
MluI	ACGCGT	1 cut(s)	339
MluNI	TGGCCA	2 cut(s)	136, 636
MlyI	GAGTC	1 cut(s)	116
MmeI	TCCRAC	2 cut(s)	135, 1134
MnlI	CCTC	6 cut(s)	192, 247, 445, 499, 675, 747
Mox20I	TGGCCA	2 cut(s)	136, 636
MroXI	GAANNNNTTC	2 cut(s)	237, 670
MscI	TGGCCA	2 cut(s)	136, 636
MseI	TTAA	2 cut(s)	719, 758
MslI	CAYNNNNRTG	3 cut(s)	14, 60, 869
Msp20I	TGGCCA	2 cut(s)	136, 636
MspA1I	CMGCKG	2 cut(s)	210, 399
MvnI	CGCG	1 cut(s)	341
MwoI	GCNNNNNNNGC	5 cut(s)	169, 207, 423, 483, 1051
NcoI	CCATGG	1 cut(s)	292
NdeI	CATATG	1 cut(s)	131
NdeII	GATC	4 cut(s)	222, 481, 745, 762
NmuCI	GTSAC	3 cut(s)	392, 860, 982
NspI	RCATGY	1 cut(s)	261
NspV	TTCGAA	1 cut(s)	660
PaeI	GCATGC	1 cut(s)	261
PagI	TCATGA	1 cut(s)	325
PcsI	WCGNNNNNNNCGW	2 cut(s)	666, 852
PdmI	GAANNNNTTC	2 cut(s)	237, 670
PfeI	GAWTC	4 cut(s)	273, 302, 671, 839
PkrI	GCNGC	2 cut(s)	401, 902
PleI	GAGTC	1 cut(s)	115
PpsI	GAGTC	1 cut(s)	115
Psp1406I	AACGTT	1 cut(s)	855
PspEI	GGTNACC	1 cut(s)	982
PspPI	GGNCC	3 cut(s)	734, 775, 1080
PstNI	CAGNNNCTG	1 cut(s)	474
PvuII	CAGCTG	1 cut(s)	399
RseI	CAYNNNNRTG	3 cut(s)	14, 60, 869
SalI	GTCGAC	1 cut(s)	1004
SaqAI	TTAA	2 cut(s)	719, 758
SatI	GCNGC	2 cut(s)	400, 901
Sau3AI	GATC	4 cut(s)	222, 481, 745, 762
Sau96I	GGNCC	3 cut(s)	734, 775, 1080
SchI	GAGTC	1 cut(s)	116
SduI	GDGCHC	2 cut(s)	379, 494
SfaNI	GCATC	4 cut(s)	76, 205, 778, 1090
SfcI	CTRYAG	1 cut(s)	1075
SfuI	TTCGAA	1 cut(s)	660
SinI	GGWCC	2 cut(s)	734, 775
SmiMI	CAYNNNNRTG	3 cut(s)	14, 60, 869
SphI	GCATGC	1 cut(s)	261
Sse9I	AATT	4 cut(s)	125, 237, 545, 653
SsiI	CCGC	2 cut(s)	210, 402
SspMI	CTAG	6 cut(s)	411, 614, 626, 725, 1047, 1055
StyI	CCWWGG	6 cut(s)	292, 446, 625, 724, 1019, 1095
TaaI	ACNGT	2 cut(s)	499, 743
TaiI	ACGT	1 cut(s)	858
TaqI	TCGA	5 cut(s)	84, 249, 504, 660, 1005
TasI	AATT	4 cut(s)	125, 237, 545, 653
TfiI	GAWTC	4 cut(s)	273, 302, 671, 839
Tru1I	TTAA	2 cut(s)	719, 758
Tru9I	TTAA	2 cut(s)	719, 758
TscAI	CASTG	5 cut(s)	427, 484, 494, 746, 1015
TseFI	GTSAC	3 cut(s)	392, 860, 982
TseI	GCWGC	2 cut(s)	399, 900
Tsp45I	GTSAC	3 cut(s)	392, 860, 982
TspDTI	ATGAA	8 cut(s)	24, 78, 118, 164, 342, 368, 596, 822
TspRI	CASTG	5 cut(s)	427, 484, 494, 746, 1015
VpaK11BI	GGWCC	2 cut(s)	734, 775
XagI	CCTNNNNNAGG	1 cut(s)	770
XapI	RAATTY	2 cut(s)	545, 653
XceI	RCATGY	1 cut(s)	261
XmaJI	CCTAGG	2 cut(s)	625, 724
XmiI	GTMKAC	1 cut(s)	1005
XmnI	GAANNNNTTC	2 cut(s)	237, 670
XspI	CTAG	6 cut(s)	411, 614, 626, 725, 1047, 1055

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.