AT4G32551

LisH

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0000670
Physical Location & Seq
Forward (+)
15707425 .. 15713662
6238 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G32551.1

Sequence Viewer

Length: 2796 bp
ATGTCTCAGACCAACTGGGAAGCTGATAAAATGTTGGATGTCTATATCCATGATTATCTGGTTAAAAGAGATTTGAAAGCTACAGCTCAGGCTTTCCAAGCTGAAGGAAAAGTGTCATCGGATCCAGTTGCTATTGACGCACCTGGTGGATTTCTGTTTGAGTGGTGGTCTGTCTTCTGGGATATATTTATTGCTAGGACCAATGAGAAGCATTCCGAGGTTGCTGCATCTTACATAGAGACACAGATGATCAAAGCACGAGAACAGCAATTGCAGCAATCTCAACACCCACAAGTTTCACAGCAGCAGCAGCAACAACAACAACAGCAGATTCAAATGCAACAACTCTTGTTGCAACGTGCACAGCAACAGCAGCAGCAGCAACAACAACAACATCATCATCATCAGCAACAGCAACAGCAACAGCAGCAGCAACAGCAACAGCAGCAGCAGCAGCAACAACAACATCAAAACCAACCACCTTCTCAACAGCAGCAGCAGCAATCAACGCCTCAACACCAACAGCAGCCTACACCACAGCAGCAGCCTCAGAGAAGAGACGGGTCCCACCTTGCAAATGGATCAGCAAATGGACTCGTTGGTAATAACAGTGAACCAGTTATGAGGCAAAATCCTGGGTCTGGGAGTTCCTTAGCTAGTAAAGCATATGAGGAGCGTGTAAAAATGCCTACTCAGAGGGAGTCCTTAGATGAGGCTGCTATGAAGCGATTTGGGGACAATGTTGGACAACTGTTGGACCCAAGTCATGCATCAATATTGAAATCTGCTGCAGCCTCTGGACAGCCTGCAGGGCAAGTGTTACACAGTACAAGTGGTGGTATGTCTCCACAGGTTCAAACTCGAAATCAGCAACTTCCTGGGTCTGCAGTGGATATTAAAAGTGAGATCAATCCCGTGCTCACTCCCAGAACTGCTGTTCCAGAAGGATCGTTGATTGGAATTCCTGGTTCAAATCAAGGCAGCAACAACCTTACACTTAAAGGGTGGCCACTCACTGGATTTGATCAGCTTCGTTCTGGTCTTCTTCAGCAGCAAAAACCATTTATGCAGTCTCAGTCTTTTCACCAACTCAACATGCTAACTCCTCAGCATCAGCAACAGCTCATGCTGGCTCAACAGAATCTAAATTCACAGTCCGTCAGTGAAGAGAACAGAAGACTGAAAATGCTATTGAACAACCGGAGCATGACCCTAGGAAAGGATGGCCTTGGGAGTTCTGTAGGGGATGTGTTACCAAATGTCGGATCGTCATTACAACCTGGTGGCTCTCTTTTGCCTCGTGGAGACACCGATATGCTACTTAAGTTAAAGATGGCTCTGTTACAGCAGCAGCAGCAAAATCAACAGCAGGGTGGTGGGAATCCACCACAGCCACAGCCGCAGCCACAACCACTTAATCAGCTTGCCCTTACAAATCCGCAACCACAGAGTTCAAATCACAGTATTCATCAACAAGAGAAATTGGGAGGTGGTGGTAGTATTACGATGGATGGTAGCATTTCAAATTCTTTTCGAGGAAACGAACAGGTATTGAAGAATCAGAGTGGGAGGAAAAGAAAGCAACCGGTTTCGTCTTCTGGACCAGCTAATAGTTCAGGAACAGCTAACACTGCGGGACCATCACCAAGCTCAGCACCTTCTACACCTTCAACTCATACTCCTGGAGATGTGATCTCTATGCCTAATCTGCCGCACAGTGGTGGTTCCTCCAAATCAATGATGATGTTTGGCACTGAAGGAACTGGGACTCTTACATCTCCATCAAATCAGTTGGCTGATATGGATCGATTTGTGGAAGATGGATCACTTGACGACAATGTTGAGTCTTTTTTATCCCAGGAGGATGGTGATCAACGGGACGCTGTTACACGGTGTATGGATGTTAGTAAAGGTTTTACGTTTACTGAAGTGAATTCAGTACGTGCGAGCACAACCAAAGTTACCTGTTGCCACTTCTCATCGGATGGAAAAATGCTTGCTAGTGCTGGACATGACAAAAAGGCTGTACTATGGTACACAGACACTATGAAGCCAAAGACGACCCTAGAGGAGCACACAGCTATGATAACAGATATCCGTTTTAGTCCTAGCCAGCTTCGCCTTGCGACTTCTTCGTTTGACAAGACTGTCAGGGTTTGGGATGCTGATAATAAAGGTTATTCCCTTCGTACTTTCATGGGACATTCTTCTATGGTTACATCACTTGACTTCCATCCCATCAAGGATGATCTTATATGTTCCTGTGACAATGATAACGAAATAAGGTACTGGAGTATCAACAATGGAAGTTGCACAAGAGTGTACAAGGGTGGTTCCACCCAGATAAGGTTCCAACCACGTGTTGGAAAGTATCTTGCGGCTTCATCAGCAAATCTTGTCAATGTACTAGATGTAGAAACACAAGCTATTCGACACTCTTTACAGGGGCATGCTAATCCGATAAACTCGGTTTGCTGGGATCCCTCTGGTGACTTCCTGGCATCTGTTAGTGAAGATATGGTAAAAGTATGGACGCTGGGGACAGGTAGTGAAGGAGAATGTGTTCATGAGCTAAGCTGCAACGGGAACAAATTCCAGTCATGTGTTTTCCATCCGGCTTACCCCTCGCTGCTCGTAATTGGTTGCTACCAGTCTTTAGAACTATGGAACATGTCAGAGAACAAGACGATGACATTACCAGCTCACGAAGGGCTAATTACATCTTTGGCTGTTTCAACTGCAACTGGATTGGTTGCATCAGCTAGTCATGATAAGCTAGTGAAACTGTGGAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0001101 GO:0001558 GO:0001666 GO:0003002 GO:0003006 GO:0005975 GO:0005976 GO:0006355 GO:0006950 GO:0006974 GO:0006979 GO:0007275 GO:0007389 GO:0008150 GO:0008152 GO:0009414 GO:0009415 GO:0009605 GO:0009607 GO:0009611 GO:0009617 GO:0009620 GO:0009624 GO:0009628 GO:0009636 GO:0009719 GO:0009725 GO:0009733 GO:0009791 GO:0009798 GO:0009845 GO:0009888 GO:0009889 GO:0009890 GO:0009892 GO:0009909 GO:0009943 GO:0009944 GO:0009955 GO:0009987 GO:0010033 GO:0010035 GO:0010038 GO:0010073 GO:0010154 GO:0010191 GO:0010214 GO:0010243 GO:0010272 GO:0010393 GO:0010468 GO:0010556 GO:0010558 GO:0010605 GO:0010629 GO:0014070 GO:0019219 GO:0019222 GO:0022414 GO:0022603 GO:0022604 GO:0030307 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0032501 GO:0032502 GO:0032504 GO:0033554 GO:0036293 GO:0040008 GO:0042221 GO:0042493 GO:0043170 GO:0043207 GO:0044237 GO:0044238 GO:0045892 GO:0045927 GO:0045934 GO:0045995 GO:0046677 GO:0046898 GO:0048316 GO:0048359 GO:0048507 GO:0048509 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048580 GO:0048608 GO:0048609 GO:0048638 GO:0048639 GO:0048731 GO:0048831 GO:0048856 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051094 GO:0051128 GO:0051130 GO:0051171 GO:0051172 GO:0051239 GO:0051252 GO:0051253 GO:0051510 GO:0051512 GO:0051704 GO:0051707 GO:0051716 GO:0060255 GO:0060992 GO:0061458 GO:0065001 GO:0065007 GO:0070482 GO:0071216 GO:0071217 GO:0071496 GO:0071704 GO:0080001 GO:0080090 GO:0090351 GO:0097305 GO:1901654 GO:1901698 GO:1901700 GO:1902074 GO:1902183 GO:1902679 GO:1903506 GO:1903507 GO:2000024 GO:2000026 GO:2000112 GO:2000113 GO:2000241 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LisH WD40

Protein Analysis

931

Amino Acids

102.23

Weight (kDa)

6.57

Isoelectric Point (pI)

57.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LisH PF08513 10 - 36 7e-07 LisH
WD40_CDC20-Fz PF24807 627 - 705 1.3e-07 CDC20/Fizzy WD40 domain
Beta-prop_THOC3 PF25174 634 - 703 7.2e-09 THOC3 beta-propeller domain
Beta-prop_EIPR1 PF23609 638 - 764 3.5e-06 EIPR1 beta-propeller
WD40_MABP1-WDR62_2nd PF24782 640 - 846 1.5e-14 MABP1/WDR62 second WD40 domain
Beta-prop_TEP1_2nd PF25047 640 - 682 6.1e-06 TEP-1 second beta-propeller
Beta-prop_WDR3_1st PF25173 647 - 766 1.1e-21 WDR3 first beta-propeller domain
WD40_Gbeta PF25391 647 - 758 1.2e-09 G protein beta WD-40 repeat protein
Beta-prop_CAF1B_HIR1 PF24105 647 - 772 4.9e-08 CAF1B/HIR1 beta-propeller domain
Beta-prop_EML_2 PF23414 648 - 770 1.4e-17 Echinoderm microtubule-associated protein second beta-propeller
WD40_WDHD1_1st PF24817 648 - 725 2.4e-12 WDHD1 first WD40 domain
Beta-prop_WDR5 PF25175 649 - 777 4.3e-29 WDR5 beta-propeller domain
Beta-prop_WDR36-Utp21_2nd PF25168 650 - 751 4e-09 WDR36/Utp21 second beta-propeller domain
WD40_Prp19 PF24814 650 - 847 1.7e-31 Prp19 WD40 domain
Beta-prop_EML PF23409 652 - 772 9.5e-06 Echinoderm microtubule-associated protein first beta-propeller
Beta-prop_WDR3_2nd PF25172 652 - 766 1.8e-13 WDR3 second beta-propeller domain
EIF3I PF24805 653 - 723 6.5e-07 EIF3I
Beta-prop_TEP1_2nd PF25047 681 - 777 1.8e-07 TEP-1 second beta-propeller
WD40 PF00400 684 - 721 2.1e-08 WD domain, G-beta repeat
WD40_CDC20-Fz PF24807 686 - 844 1.1e-17 CDC20/Fizzy WD40 domain
Beta-prop_THOC3 PF25174 692 - 931 7.3e-38 THOC3 beta-propeller domain
Beta-prop_WDR3_1st PF25173 730 - 890 4.9e-17 WDR3 first beta-propeller domain
WDR55 PF24796 736 - 897 4e-06 WDR55
Beta-prop_Aladin PF25460 737 - 845 8.1e-06 Aladin seven-bladed propeller
WD40_Gbeta PF25391 749 - 930 8e-07 G protein beta WD-40 repeat protein
Beta-prop_WDR36-Utp21_2nd PF25168 752 - 850 4.7e-07 WDR36/Utp21 second beta-propeller domain
Beta-prop_WDR5 PF25175 778 - 930 3.4e-25 WDR5 beta-propeller domain
WD40_WDHD1_1st PF24817 780 - 931 8.5e-14 WDHD1 first WD40 domain
WD40_Prp19 PF24814 780 - 930 8.5e-16 Prp19 WD40 domain
Beta-prop_WDR36-Utp21_1st PF25171 791 - 925 1.1e-06 WDR36/Utp21 first beta-propeller
Beta-prop_WDR3_2nd PF25172 792 - 931 1.5e-08 WDR3 second beta-propeller domain
WD40_CDC20-Fz PF24807 793 - 930 1.6e-07 CDC20/Fizzy WD40 domain
Beta-prop_EIPR1 PF23609 794 - 904 3.3e-06 EIPR1 beta-propeller
WD40 PF00400 811 - 844 5.3e-07 WD domain, G-beta repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000670)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G32551 AT4G32551
fragaria_vesca FvH4_1g04170 FvH4_1g04170 FvH4_1g04170 FvH4_1g04170 FvH4_1g04170 FvH4_1g04170 FvH4_1g04170 FvH4_1g04170 FvH4_2g29940 FvH4_2g29940 FvH4_2g29940 FvH4_2g29940 FvH4_2g29940 FvH4_2g29940 FvH4_2g29940 FvH4_2g29940
malus_domestica MD02G1040200.v1.1 MD08G1139100.v1.1 MD15G1117100.v1.1 MD15G1145100.v1.1
prunus_persica Prupe.1G468400_v2.0.a1 Prupe.1G468400_v2.0.a1 Prupe.1G468400_v2.0.a1 Prupe.1G468400_v2.0.a1 Prupe.1G468400_v2.0.a1 Prupe.1G468400_v2.0.a1 Prupe.1G468400_v2.0.a1 Prupe.1G468400_v2.0.a1 Prupe.1G468400_v2.0.a1 Prupe.1G468400_v2.0.a1 Prupe.1G468400_v2.0.a1 Prupe.1G468400_v2.0.a1 Prupe.1G468400_v2.0.a1 Prupe.1G468400_v2.0.a1 Prupe.1G468400_v2.0.a1 Prupe.1G468400_v2.0.a1 Prupe.1G468400_v2.0.a1 Prupe.7G235500_v2.0.a1 Prupe.7G235500_v2.0.a1 Prupe.7G235500_v2.0.a1 Prupe.7G235500_v2.0.a1
pyrus_communis pycom02g03350 pycom08g11750 pycom15g10550 pycom15g16250
rosa_chinensis RchiOBHm_Chr2g0089671 RchiOBHm_Chr3g0465211 RchiOBHm_Chr6g0312941
rosa_laevigata RLG00000010283 RLG00000016050
rosa_multiflora Rmu_sc0000665.1_g000023 Rmu_sc0008049.1_g000043 Rmu_sc0008049.1_g000044
rosa_roxburghii Rroxscaffold_2G00151660 Rroxscaffold_7G00156310
rosa_rugosa Rorug02G0002500 Rorug02G0002500 Rorug06G0400700 Rorug06G0400700 Rorug06G0400700 Rorug06G0400700 Rorug06G0400800.1 Rorug06G0400900.1
rosa_samantha Rh2AG047900 Rh2BG046500 Rh2CG048500 Rh2DG047900 Rh6AG517700 Rh6BG529400 Rh6CG533900 Rh6DG520500
rosa_wichuraiana Rw2G004260 Rw5G017770 Rw6G045010

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 2147
AccB7I CCANNNNNTGG 2 cut(s) 1016, 2363
AciI CCGC 5 cut(s) 1400, 1439, 1634, 1712, 2378
AclWI GGATC 9 cut(s) 116, 129, 589, 955, 1273, 1812, 1831, 2474, 2487
AcoI YGGCCR 1 cut(s) 1007
AcsI RAATTY 5 cut(s) 960, 1147, 1525, 1933, 2591
AcuI CTGAAG 4 cut(s) 123, 1031, 1776, 1947
AcvI CACGTG 1 cut(s) 2360
AdeI CACNNNGTG 1 cut(s) 146
AfaI GTAC 8 cut(s) 829, 1941, 2028, 2036, 2191, 2288, 2324, 2406
AfiI CCNNNNNNNGG 7 cut(s) 641, 1016, 1219, 1262, 1718, 2346, 2363
AflII CTTAAG 1 cut(s) 1322
AflIII ACRYGT 2 cut(s) 2359, 2670
AgeI ACCGGT 1 cut(s) 1585
AjnI CCWGG 8 cut(s) 142, 634, 877, 964, 1279, 1681, 1857, 2496
AleI CACNNNNGTG 2 cut(s) 1719, 2318
AloI GAACNNNNNNTCC 4 cut(s) 922, 954, 2547, 2579
Alw21I GWGCWC 4 cut(s) 364, 921, 1952, 2076
Alw26I GTCTC 6 cut(s) 9, 233, 552, 849, 1077, 1299
Alw44I GTGCAC 1 cut(s) 360
AlwI GGATC 9 cut(s) 116, 129, 589, 955, 1273, 1812, 1831, 2474, 2487
AlwNI CAGNNNCTG 1 cut(s) 797
AoxI GGCC 2 cut(s) 1007, 1225
ApaLI GTGCAC 1 cut(s) 360
ApoI RAATTY 5 cut(s) 960, 1147, 1525, 1933, 2591
AsiGI ACCGGT 1 cut(s) 1585
Asp700I GAANNNNTTC 2 cut(s) 2562, 2591
AspA2I CCTAGG 1 cut(s) 1213
AspS9I GGNCC 5 cut(s) 198, 564, 757, 1601, 1637
AsuHPI GGTGA 4 cut(s) 1076, 1635, 1880, 2501
AvaII GGWCC 5 cut(s) 198, 564, 757, 1601, 1637
AvrII CCTAGG 1 cut(s) 1213
BaeGI GKGCMC 1 cut(s) 364
BaeI ACNNNNGTAYC 4 cut(s) 2278, 2278, 2311, 2311
BalI TGGCCA 1 cut(s) 1009
BamHI GGATCC 2 cut(s) 121, 2479
BauI CACGAG 2 cut(s) 258, 1299
BbrPI CACGTG 1 cut(s) 2360
BbsI GAAGAC 4 cut(s) 166, 1034, 1183, 1587
Bbv12I GWGCWC 4 cut(s) 364, 921, 1952, 2076
BbvCI CCTCAGC 1 cut(s) 1107
BcgI CGANNNNNNTGC 2 cut(s) 206, 240
BciT130I CCWGG 8 cut(s) 144, 636, 879, 966, 1281, 1683, 1859, 2498
BclI TGATCA 3 cut(s) 249, 1024, 1870
BcoDI GTCTC 6 cut(s) 9, 233, 552, 849, 1077, 1299
BfaI CTAG 9 cut(s) 195, 657, 1214, 2001, 2066, 2109, 2408, 2763, 2777
BfmI CTRYAG 5 cut(s) 81, 789, 807, 885, 1239
BfrI CTTAAG 1 cut(s) 1322
BglI GCCNNNNNGGC 1 cut(s) 811
BlnI CCTAGG 1 cut(s) 1213
BlpI GCTNAGC 2 cut(s) 1651, 2573
Bme1390I CCNGG 8 cut(s) 144, 636, 879, 966, 1281, 1683, 1859, 2498
Bme18I GGWCC 5 cut(s) 198, 564, 757, 1601, 1637
BmgT120I GGNCC 5 cut(s) 198, 564, 757, 1601, 1637
BmiI GGNNCC 9 cut(s) 123, 565, 566, 759, 1638, 1726, 2335, 2351, 2481
BmrFI CCNGG 8 cut(s) 144, 636, 879, 966, 1281, 1683, 1859, 2498
BmrI ACTGGG 2 cut(s) 25, 1773
BmsI GCATC 6 cut(s) 236, 779, 1120, 2152, 2510, 2765
BmuI ACTGGG 2 cut(s) 25, 1773
BoxI GACNNNNGTC 1 cut(s) 762
BpiI GAAGAC 4 cut(s) 166, 1034, 1183, 1587
BpmI CTGGAG 2 cut(s) 1704, 2311
Bpu10I CCTNAGC 3 cut(s) 87, 652, 1107
Bpu1102I GCTNAGC 2 cut(s) 1651, 2573
Bsa29I ATCGAT 1 cut(s) 1807
BsaAI YACGTR 2 cut(s) 1943, 2360
BsaBI GATNNNNATC 2 cut(s) 1803, 1869
BsaJI CCNNGG 6 cut(s) 216, 635, 878, 1213, 1228, 1857
BsaWI WCCGGW 2 cut(s) 1200, 1585
BsaXI ACNNNNNCTCC 4 cut(s) 1663, 1693, 2547, 2577
Bsc4I CCNNNNNNNGG 7 cut(s) 641, 1016, 1219, 1262, 1718, 2346, 2363
Bse118I RCCGGY 1 cut(s) 1585
Bse1I ACTGG 9 cut(s) 20, 125, 617, 1021, 1768, 2294, 2596, 2650, 2749
Bse8I GATNNNNATC 2 cut(s) 1803, 1869
BseBI CCWGG 8 cut(s) 144, 636, 879, 966, 1281, 1683, 1859, 2498
BseCI ATCGAT 1 cut(s) 1807
BseDI CCNNGG 6 cut(s) 216, 635, 878, 1213, 1228, 1857
BseJI GATNNNNATC 2 cut(s) 1803, 1869
BseLI CCNNNNNNNGG 7 cut(s) 641, 1016, 1219, 1262, 1718, 2346, 2363
BseMII CTCAG 7 cut(s) 20, 101, 563, 707, 1088, 1121, 1665
BseNI ACTGG 9 cut(s) 20, 125, 617, 1021, 1768, 2294, 2596, 2650, 2749
BseRI GAGGAG 3 cut(s) 686, 1095, 2084
BseSI GKGCMC 1 cut(s) 364
BseYI CCCAGC 2 cut(s) 2475, 2536
BshFI GGCC 2 cut(s) 1009, 1227
BshTI ACCGGT 1 cut(s) 1585
BshVI ATCGAT 1 cut(s) 1807
BsiHKAI GWGCWC 4 cut(s) 364, 921, 1952, 2076
BsiSI CCGG 3 cut(s) 1201, 1586, 2615
BslFI GGGAC 7 cut(s) 550, 749, 1650, 1780, 1892, 2214, 2554
BslI CCNNNNNNNGG 7 cut(s) 641, 1016, 1219, 1262, 1718, 2346, 2363
BsmAI GTCTC 6 cut(s) 9, 233, 552, 849, 1077, 1299
BsmBI CGTCTC 1 cut(s) 552
BsmFI GGGAC 7 cut(s) 550, 749, 1650, 1780, 1892, 2214, 2554
BsmI GAATGC 1 cut(s) 211
BsnI GGCC 2 cut(s) 1009, 1227
Bsp1286I GDGCHC 4 cut(s) 364, 921, 1952, 2076
Bsp1407I TGTACA 1 cut(s) 2322
Bsp1720I GCTNAGC 2 cut(s) 1651, 2573
BspACI CCGC 5 cut(s) 1400, 1439, 1634, 1712, 2378
BspANI GGCC 2 cut(s) 1009, 1227
BspCNI CTCAG 7 cut(s) 19, 100, 562, 706, 1087, 1120, 1664
BspDI ATCGAT 1 cut(s) 1807
BspHI TCATGA 2 cut(s) 2566, 2767
BspLI GGNNCC 9 cut(s) 123, 565, 566, 759, 1638, 1726, 2335, 2351, 2481
BspMAI CTGCAG 3 cut(s) 793, 811, 889
BspPI GGATC 9 cut(s) 116, 129, 589, 955, 1273, 1812, 1831, 2474, 2487
BspTI CTTAAG 1 cut(s) 1322
BsrFI RCCGGY 1 cut(s) 1585
BsrGI TGTACA 1 cut(s) 2322
BsrI ACTGG 9 cut(s) 20, 125, 617, 1021, 1768, 2294, 2596, 2650, 2749
BssAI RCCGGY 1 cut(s) 1585
BssECI CCNNGG 6 cut(s) 216, 635, 878, 1213, 1228, 1857
BssSI CACGAG 2 cut(s) 258, 1299
BssT1I CCWWGG 2 cut(s) 1213, 1228
Bst2BI CACGAG 2 cut(s) 258, 1299
Bst2UI CCWGG 8 cut(s) 144, 636, 879, 966, 1281, 1683, 1859, 2498
Bst4CI ACNGT 9 cut(s) 611, 753, 827, 1155, 1463, 1718, 1893, 2149, 2787
Bst6I CTCTTC 2 cut(s) 550, 1161
BstAFI CTTAAG 1 cut(s) 1322
BstAUI TGTACA 1 cut(s) 2322
BstBAI YACGTR 2 cut(s) 1943, 2360
BstC8I GCNNGC 7 cut(s) 807, 1131, 1425, 1948, 1998, 2114, 2451
BstENI CCTNNNNNAGG 1 cut(s) 1217
BstMAI GTCTC 6 cut(s) 9, 233, 552, 849, 1077, 1299
BstNI CCWGG 8 cut(s) 144, 636, 879, 966, 1281, 1683, 1859, 2498
BstNSI RCATGY 3 cut(s) 1099, 2453, 2674
BstPAI GACNNNNGTC 1 cut(s) 762
BstSCI CCNGG 8 cut(s) 142, 634, 877, 964, 1279, 1681, 1857, 2496
BstSFI CTRYAG 5 cut(s) 81, 789, 807, 885, 1239
BstSLI GKGCMC 1 cut(s) 364
BstV2I GAAGAC 4 cut(s) 166, 1034, 1183, 1587
BstX2I RGATCY 2 cut(s) 121, 2479
BstXI CCANNNNNNTGG 1 cut(s) 1865
BstYI RGATCY 2 cut(s) 121, 2479
Bsu15I ATCGAT 1 cut(s) 1807
BsuRI GGCC 2 cut(s) 1009, 1227
BsuTUI ATCGAT 1 cut(s) 1807
BtsI GCAGTG 2 cut(s) 894, 1629
BtsIMutI CAGTG 7 cut(s) 616, 894, 1014, 1168, 1629, 1723, 1752
Cac8I GCNNGC 7 cut(s) 807, 1131, 1425, 1948, 1998, 2114, 2451
CaiI CAGNNNCTG 1 cut(s) 797
CciI TCATGA 2 cut(s) 2566, 2767
Cfr10I RCCGGY 1 cut(s) 1585
Cfr13I GGNCC 5 cut(s) 198, 564, 757, 1601, 1637
ClaI ATCGAT 1 cut(s) 1807
CseI GACGC 3 cut(s) 146, 1889, 2542
CsiI ACCWGGT 2 cut(s) 142, 1279
Csp6I GTAC 8 cut(s) 828, 1940, 2027, 2035, 2190, 2287, 2323, 2405
CspAI ACCGGT 1 cut(s) 1585
CviQI GTAC 8 cut(s) 828, 1940, 2027, 2035, 2190, 2287, 2323, 2405
DraIII CACNNNGTG 1 cut(s) 146
DrdI GACNNNNNNGTC 1 cut(s) 2147
DseDI GACNNNNNNGTC 1 cut(s) 2147
EaeI YGGCCR 1 cut(s) 1007
Eam1104I CTCTTC 2 cut(s) 550, 1161
EarI CTCTTC 2 cut(s) 550, 1161
Eco130I CCWWGG 2 cut(s) 1213, 1228
Eco32I GATATC 1 cut(s) 2095
Eco47I GGWCC 5 cut(s) 198, 564, 757, 1601, 1637
Eco57I CTGAAG 4 cut(s) 123, 1031, 1776, 1947
Eco72I CACGTG 1 cut(s) 2360
EcoNI CCTNNNNNAGG 1 cut(s) 1217
EcoO109I RGGNCCY 1 cut(s) 564
EcoRI GAATTC 2 cut(s) 960, 1933
EcoRII CCWGG 8 cut(s) 142, 634, 877, 964, 1279, 1681, 1857, 2496
EcoRV GATATC 1 cut(s) 2095
EcoT14I CCWWGG 2 cut(s) 1213, 1228
EcoT22I ATGCAT 1 cut(s) 772
ErhI CCWWGG 2 cut(s) 1213, 1228
Esp3I CGTCTC 1 cut(s) 552
FaqI GGGAC 7 cut(s) 550, 749, 1650, 1780, 1892, 2214, 2554
FauI CCCGC 1 cut(s) 1627
FauNDI CATATG 1 cut(s) 667
FbaI TGATCA 3 cut(s) 249, 1024, 1870
FspBI CTAG 9 cut(s) 195, 657, 1214, 2001, 2066, 2109, 2408, 2763, 2777
GsaI CCCAGC 2 cut(s) 2479, 2540
GsuI CTGGAG 2 cut(s) 1704, 2311
HaeIII GGCC 2 cut(s) 1009, 1227
HapII CCGG 3 cut(s) 1201, 1586, 2615
HgaI GACGC 3 cut(s) 146, 1889, 2542
HinfI GANTC 8 cut(s) 331, 594, 701, 1141, 1381, 1558, 1768, 1844
HpaII CCGG 3 cut(s) 1201, 1586, 2615
HphI GGTGA 4 cut(s) 1076, 1635, 1880, 2501
Hpy166II GTNNAC 5 cut(s) 362, 614, 1923, 2037, 2323
Hpy188III TCNNGA 7 cut(s) 798, 941, 1599, 1617, 2567, 2705, 2768
Hpy8I GTNNAC 5 cut(s) 362, 614, 1923, 2037, 2323
HpyAV CCTTC 9 cut(s) 98, 492, 938, 1668, 1677, 1751, 2195, 2546, 2702
HpyCH4III ACNGT 9 cut(s) 611, 753, 827, 1155, 1463, 1718, 1893, 2149, 2787
HpyCH4IV ACGT 4 cut(s) 358, 1919, 1942, 2359
HpySE526I ACGT 4 cut(s) 358, 1919, 1942, 2359
KflI GGGWCCC 1 cut(s) 564
Ksp22I TGATCA 3 cut(s) 249, 1024, 1870
LmnI GCTCC 3 cut(s) 673, 1203, 2071
LweI GCATC 6 cut(s) 236, 779, 1120, 2152, 2510, 2765
MabI ACCWGGT 2 cut(s) 142, 1279
MaeI CTAG 9 cut(s) 195, 657, 1214, 2001, 2066, 2109, 2408, 2763, 2777
MaeII ACGT 4 cut(s) 358, 1919, 1942, 2359
MaeIII GTNAC 8 cut(s) 819, 1251, 1341, 1885, 1960, 2215, 2264, 2489
MfeI CAATTG 1 cut(s) 269
MflI RGATCY 2 cut(s) 121, 2479
MhlI GDGCHC 4 cut(s) 364, 921, 1952, 2076
MlsI TGGCCA 1 cut(s) 1009
MluCI AATT 9 cut(s) 269, 960, 1147, 1481, 1525, 1933, 2591, 2637, 2715
MluNI TGGCCA 1 cut(s) 1009
MlyI GAGTC 4 cut(s) 588, 710, 1762, 1853
MmeI TCCRAC 6 cut(s) 15, 724, 735, 1243, 2344, 2377
Mox20I TGGCCA 1 cut(s) 1009
Mph1103I ATGCAT 1 cut(s) 772
MroXI GAANNNNTTC 2 cut(s) 2562, 2591
MscI TGGCCA 1 cut(s) 1009
MseI TTAA 6 cut(s) 63, 897, 999, 1323, 1328, 1416
MslI CAYNNNNRTG 4 cut(s) 1313, 1719, 2081, 2318
Msp20I TGGCCA 1 cut(s) 1009
MspCI CTTAAG 1 cut(s) 1322
MspI CCGG 3 cut(s) 1201, 1586, 2615
MspR9I CCNGG 8 cut(s) 144, 636, 879, 966, 1281, 1683, 1859, 2498
MunI CAATTG 1 cut(s) 269
Mva1269I GAATGC 1 cut(s) 211
MvaI CCWGG 8 cut(s) 144, 636, 879, 966, 1281, 1683, 1859, 2498
NdeI CATATG 1 cut(s) 667
NlaIV GGNNCC 9 cut(s) 123, 565, 566, 759, 1638, 1726, 2335, 2351, 2481
NmuCI GTSAC 2 cut(s) 2264, 2489
NsiI ATGCAT 1 cut(s) 772
NspI RCATGY 3 cut(s) 1099, 2453, 2674
OliI CACNNNNGTG 2 cut(s) 1719, 2318
PaeI GCATGC 1 cut(s) 2453
PagI TCATGA 2 cut(s) 2566, 2767
PciI ACATGT 1 cut(s) 2670
PctI GAATGC 1 cut(s) 211
PdmI GAANNNNTTC 2 cut(s) 2562, 2591
PfeI GAWTC 4 cut(s) 331, 1141, 1381, 1558
PflMI CCANNNNNTGG 2 cut(s) 1016, 2363
PfoI TCCNGGA 1 cut(s) 1681
PinAI ACCGGT 1 cut(s) 1585
PleI GAGTC 4 cut(s) 588, 709, 1762, 1852
PmaCI CACGTG 1 cut(s) 2360
PmlI CACGTG 1 cut(s) 2360
PpsI GAGTC 4 cut(s) 588, 709, 1762, 1852
Ppu21I YACGTR 2 cut(s) 1943, 2360
PpuMI RGGWCCY 1 cut(s) 564
PscI ACATGT 1 cut(s) 2670
PshAI GACNNNNGTC 1 cut(s) 762
Psp5II RGGWCCY 1 cut(s) 564
Psp6I CCWGG 8 cut(s) 142, 634, 877, 964, 1279, 1681, 1857, 2496
PspCI CACGTG 1 cut(s) 2360
PspFI CCCAGC 2 cut(s) 2475, 2536
PspGI CCWGG 8 cut(s) 142, 634, 877, 964, 1279, 1681, 1857, 2496
PspN4I GGNNCC 9 cut(s) 123, 565, 566, 759, 1638, 1726, 2335, 2351, 2481
PspPI GGNCC 5 cut(s) 198, 564, 757, 1601, 1637
PspPPI RGGWCCY 1 cut(s) 564
PstI CTGCAG 3 cut(s) 793, 811, 889
PstNI CAGNNNCTG 1 cut(s) 797
PsuI RGATCY 2 cut(s) 121, 2479
RsaI GTAC 8 cut(s) 829, 1941, 2028, 2036, 2191, 2288, 2324, 2406
RsaNI GTAC 8 cut(s) 828, 1940, 2027, 2035, 2190, 2287, 2323, 2405
RseI CAYNNNNRTG 4 cut(s) 1313, 1719, 2081, 2318
SaqAI TTAA 6 cut(s) 63, 897, 999, 1323, 1328, 1416
Sau96I GGNCC 5 cut(s) 198, 564, 757, 1601, 1637
SbfI CCTGCAGG 1 cut(s) 811
SchI GAGTC 4 cut(s) 588, 710, 1762, 1853
ScrFI CCNGG 8 cut(s) 144, 636, 879, 966, 1281, 1683, 1859, 2498
SdaI CCTGCAGG 1 cut(s) 811
SduI GDGCHC 4 cut(s) 364, 921, 1952, 2076
SexAI ACCWGGT 2 cut(s) 142, 1279
SfaNI GCATC 6 cut(s) 236, 779, 1120, 2152, 2510, 2765
SfcI CTRYAG 5 cut(s) 81, 789, 807, 885, 1239
SinI GGWCC 5 cut(s) 198, 564, 757, 1601, 1637
SmiMI CAYNNNNRTG 4 cut(s) 1313, 1719, 2081, 2318
SmlI CTYRAG 1 cut(s) 1322
SmoI CTYRAG 1 cut(s) 1322
SphI GCATGC 1 cut(s) 2453
Sse8387I CCTGCAGG 1 cut(s) 811
Sse9I AATT 9 cut(s) 269, 960, 1147, 1481, 1525, 1933, 2591, 2637, 2715
SsiI CCGC 5 cut(s) 1400, 1439, 1634, 1712, 2378
SspI AATATT 1 cut(s) 777
SspMI CTAG 9 cut(s) 195, 657, 1214, 2001, 2066, 2109, 2408, 2763, 2777
StyD4I CCNGG 8 cut(s) 142, 634, 877, 964, 1279, 1681, 1857, 2496
StyI CCWWGG 2 cut(s) 1213, 1228
TaaI ACNGT 9 cut(s) 611, 753, 827, 1155, 1463, 1718, 1893, 2149, 2787
TaiI ACGT 4 cut(s) 361, 1922, 1945, 2362
TaqI TCGA 4 cut(s) 862, 1534, 1807, 2431
TasI AATT 9 cut(s) 269, 960, 1147, 1481, 1525, 1933, 2591, 2637, 2715
TatI WGTACW 4 cut(s) 827, 2026, 2322, 2404
TauI GCSGC 3 cut(s) 1402, 1714, 2381
TfiI GAWTC 4 cut(s) 331, 1141, 1381, 1558
Tru1I TTAA 6 cut(s) 63, 897, 999, 1323, 1328, 1416
Tru9I TTAA 6 cut(s) 63, 897, 999, 1323, 1328, 1416
TscAI CASTG 7 cut(s) 616, 894, 1021, 1168, 1636, 1723, 1759
TseFI GTSAC 2 cut(s) 2264, 2489
Tsp45I GTSAC 2 cut(s) 2264, 2489
TspDTI ATGAA 6 cut(s) 737, 1457, 2063, 2185, 2373, 2555
TspGWI ACGGA 2 cut(s) 1147, 2087
TspRI CASTG 7 cut(s) 616, 894, 1021, 1168, 1636, 1723, 1759
Van91I CCANNNNNTGG 2 cut(s) 1016, 2363
Vha464I CTTAAG 1 cut(s) 1322
VneI GTGCAC 1 cut(s) 360
VpaK11BI GGWCC 5 cut(s) 198, 564, 757, 1601, 1637
XagI CCTNNNNNAGG 1 cut(s) 1217
XapI RAATTY 5 cut(s) 960, 1147, 1525, 1933, 2591
XceI RCATGY 3 cut(s) 1099, 2453, 2674
XcmI CCANNNNNNNNNTGG 2 cut(s) 575, 2360
XmaJI CCTAGG 1 cut(s) 1213
XmnI GAANNNNTTC 2 cut(s) 2562, 2591
XspI CTAG 9 cut(s) 195, 657, 1214, 2001, 2066, 2109, 2408, 2763, 2777
Zsp2I ATGCAT 1 cut(s) 772
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.