MD02G1040200.v1.1

Transcriptional corepressor LEUNIG-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0000670
Physical Location & Seq
Forward (+)
3269715 .. 3276515
6801 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1040200.v1.1.491

Sequence Viewer

Length: 2583 bp
ATGTCTCAGAGCCAGACCAATTGGGAAGCTGATAAAATGTTGGATGTGTATATCTATGATTACCTTATGAAGAGAAAATTACATGCTTCTGCAAAGGCATTTCAAGATGAAGGAAAAGTTTCCACGGATCCTGTAGCTATTGATGCACCTGGTGGCTTTCTTTTCGAATGGTGGTCTGTCTTTTGGGACATATTCATTGCTAGGACGAATGAGAAGCACTCAGAAGCAGCTGCATCTTACATTGAGACTCAAGTGATAAAGGCGAGGGAGCAGCAGCAGCAGCAGAAGCCTCAGCTGCAAGATCAAATGCAAATGCAGCAGCTTCTATTACAAAGGCATGCTCAGCAGCAACAGCAGCAGCGACAACAACAACAACAACAGCAGCAGCCACAGCAGCAACAGCAACAGCAGCAGCAGCGTCGAGATGGGACCCAGCTTCATAATGGCACTGCCAATGATTCTCTTTTGAGGCAGAACCCTGCAACTGCAAATTCTATGGCAACAAAAATGTACGAGGAGAGATTAAAGCTTCCACCACAGAGAGATGCTATAGATGATGCAGCTATCAAGCAAAGGTTAGGCGATAATATGAGTCAGCTTTTGGATCCCAATCATGCGTCGATGATGAAAGCAGCCACAGCAGGTGGCCAGCCTCCTGGTCAAATGCTGCATGGTACACCTGGAGGTGTGTTGGGGAATCTTCAACAACCTCACAGTCGGAGTCAGCAACTTCCTGGTTCTTCACAGGACATAAAGAGTGAGGTGATGAACCCCAGAGCTGTTGCTCCAGAAGGATCATTGATTGGCGCTCATGGATCAAATCAAGGCAATAACAATTTGACTCTGAAAGGATGGCCTTTAACGGGATTTGATCGGCTTCGATCTGGGATTCTTCAGCAGCAAAATTCTTTGATGCAGTCCCCACAACCCTATAATCAACTTCTGCAACAGCAGCAACTTATGCTAGCACAACAAAATTTGGCTTCCCCATCTTCCAATGATTTGGACAATAGGCGGATGAAAATGCTTCTCAACAATCGAAATATGGTTCTTGGGAAGGATGGTCAATTAAGTTCTGTCGATGTACCTAATGTTGGATCACCAGCGCAAGTTGGTTGCCCGGTCTTGCCTCGTGGAGATGCTGACATGCTAATGAAGTTACAGCAACAGCAGATGCAAAGCAACAATCAACAGCAGCAGCCATATTCGCAGCATCCGCTTTCAGGTCAGCATTCTCAGAATTCAAGCCAACACCTTCAGCAGCATGAAAAAATTATGGGTTCTGGCAGCATGGCGCCAGATGGTAGCATGCCTAACACCTTACAAGGGAATGATCAGGCTTCAAAGAATCAACTTGGGCGAAAGAGAAAGCAGCCAGTGTCATCTTCGGGTCCTGCCAATAGCTCAGGGACAGTTAATACCACAGGACCATCTCCCAGTTCACCTTCAACGCCTTCTACTCACACAGCAGGAGATGTCATGTCTATGCCAACTTTACCCCATAATGGTGGTTCGTCGAAGTCTCTACTTATGTTTGGCTCTGATGGCTTGGGCTCACGTGCATCAGCGCCAAATAAATTGACTGATGTAGACCGTTTTGTTGACGATGGATCTTTGGAGGATAACGTTGAGTCGTTCTTATCACATGATGATGCTGACCCTAGAGGTAGAGTCGTTCGGTGTTCAGATGTCAGCAAAGGCTTCAGTTTTAAGGAAGTCCAGCTTATTCCTGCAAGTACAAATAAAGTTGAATGTTGCCACTTATCTTCAGATGGAAAATCACTTGCCACTGGTGGGCATGACCGAAAGGCTGTATTGTGGTGCACTGAAACTTATAGTGTCAAGTCTACACTTGACGAGCATTCTCAATGGATAACTGACGTTCGCTTTAGTCCTAGCATGTCAAGGCTTGCTACATCTTCTGCTGACAAAACCGTCAGGGTTTGGGATGCTGATAACCCTGGATATTCACTACGTACTTTTACGGGACATTCTTCCACTGTCATGTCGGTGGACTTCCACCCCAGCAAAGAGGACTTTCTCTGCTCCTGTGATAACAACAGTGAGATACGGTACTGGAGTATCAAGAACGGTAGTTGTGCTGGAGTTTTCAAGGGTGGTGCAACTCAGGTGAGGTTTCAACCGTGTTTTGGAAGAAACCTTGCTGCTGCAGCAGATAATTTCGTATCCATCTTGGATGTCGAGACACAAGTTTGCAGGCTTAAGTTACAGGGTCATAAAAGCGCTGTCCATTCTGTGTGCTGGGATCCTTCTGGCGATTATCTAGCATCAGTGAGTGACGATTTGGTTCGAGTGTGGACAATTGGTTCCAGCTGCAAAGGGGAATTCATTCACGAGTTGAGCTGTTCTGGCAACAAATTCAATACGTGTGTGTTCCATCCGACTTATCCTGCATTGTTGGTCATTGGCTGTTATGAGACTTTGGAGCTTTGGAACATGACCGAGAACAAGACAATGACTCTGCACGCTCACGACAAGCTAGTGTCTTCTCTGGCGGTATCAAGTGCTACTGGGTTAGTTGCTTCAGCCAGCCATGACAAGTGCGTCAAGCTCTGGAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0001101 GO:0001558 GO:0001666 GO:0003002 GO:0003006 GO:0005975 GO:0005976 GO:0006355 GO:0006950 GO:0006974 GO:0006979 GO:0007275 GO:0007389 GO:0008150 GO:0008152 GO:0009414 GO:0009415 GO:0009605 GO:0009607 GO:0009611 GO:0009617 GO:0009620 GO:0009624 GO:0009628 GO:0009636 GO:0009719 GO:0009725 GO:0009733 GO:0009791 GO:0009798 GO:0009845 GO:0009888 GO:0009889 GO:0009890 GO:0009892 GO:0009909 GO:0009943 GO:0009944 GO:0009955 GO:0009987 GO:0010033 GO:0010035 GO:0010038 GO:0010073 GO:0010154 GO:0010191 GO:0010214 GO:0010243 GO:0010272 GO:0010393 GO:0010468 GO:0010556 GO:0010558 GO:0010605 GO:0010629 GO:0014070 GO:0019219 GO:0019222 GO:0022414 GO:0022603 GO:0022604 GO:0030307 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0032501 GO:0032502 GO:0032504 GO:0033554 GO:0036293 GO:0040008 GO:0042221 GO:0042493 GO:0043170 GO:0043207 GO:0044237 GO:0044238 GO:0045892 GO:0045927 GO:0045934 GO:0045995 GO:0046677 GO:0046898 GO:0048316 GO:0048359 GO:0048507 GO:0048509 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048580 GO:0048608 GO:0048609 GO:0048638 GO:0048639 GO:0048731 GO:0048831 GO:0048856 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051094 GO:0051128 GO:0051130 GO:0051171 GO:0051172 GO:0051239 GO:0051252 GO:0051253 GO:0051510 GO:0051512 GO:0051704 GO:0051707 GO:0051716 GO:0060255 GO:0060992 GO:0061458 GO:0065001 GO:0065007 GO:0070482 GO:0071216 GO:0071217 GO:0071496 GO:0071704 GO:0080001 GO:0080090 GO:0090351 GO:0097305 GO:1901654 GO:1901698 GO:1901700 GO:1902074 GO:1902183 GO:1902679 GO:1903506 GO:1903507 GO:2000024 GO:2000026 GO:2000112 GO:2000113 GO:2000241 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LisH WD40

Protein Analysis

861

Amino Acids

94.56

Weight (kDa)

6.97

Isoelectric Point (pI)

52.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LisH PF08513 12 - 38 3.2e-07 LisH
WD40_Gbeta PF25391 569 - 711 1.1e-10 G protein beta WD-40 repeat protein
Beta-prop_THOC3 PF25174 569 - 632 8.9e-08 THOC3 beta-propeller domain
WD40_CDC20-Fz PF24807 571 - 702 4.3e-15 CDC20/Fizzy WD40 domain
WD40_MABP1-WDR62_2nd PF24782 571 - 773 4.7e-14 MABP1/WDR62 second WD40 domain
Beta-prop_WDR3_1st PF25173 572 - 711 4.3e-22 WDR3 first beta-propeller domain
Beta-prop_TEP1_2nd PF25047 574 - 695 2e-12 TEP-1 second beta-propeller
WD40_WDHD1_1st PF24817 576 - 653 1.2e-09 WDHD1 first WD40 domain
Beta-prop_EML_2 PF23414 578 - 699 1.2e-17 Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_WDR5 PF25175 579 - 705 2.3e-24 WDR5 beta-propeller domain
Beta-prop_EML PF23409 580 - 699 3e-06 Echinoderm microtubule-associated protein first beta-propeller
WD40_Prp19 PF24814 580 - 775 4.1e-31 Prp19 WD40 domain
Beta-prop_WDR36-Utp21_2nd PF25168 580 - 684 2.8e-07 WDR36/Utp21 second beta-propeller domain
Beta-prop_WDR3_2nd PF25172 583 - 694 7.8e-12 WDR3 second beta-propeller domain
Beta-prop_CAF1B_HIR1 PF24105 587 - 653 3.8e-06 CAF1B/HIR1 beta-propeller domain
WD40 PF00400 615 - 650 6.1e-09 WD domain, G-beta repeat
Beta-prop_THOC3 PF25174 621 - 860 1.4e-35 THOC3 beta-propeller domain
Beta-prop_WDR90_POC16_2nd PF23393 632 - 699 5.2e-07 WDR90/POC16, second beta-propeller
EIF3I PF24805 638 - 706 8e-06 EIF3I
EIF3I PF24805 662 - 859 6.3e-07 EIF3I
Beta-prop_WDR36-Utp21_2nd PF25168 683 - 779 7.8e-06 WDR36/Utp21 second beta-propeller domain
Beta-prop_WDR5 PF25175 707 - 859 4.7e-24 WDR5 beta-propeller domain
WD40_WDHD1_1st PF24817 709 - 860 2.5e-12 WDHD1 first WD40 domain
WD40_Prp19 PF24814 709 - 859 2.8e-15 Prp19 WD40 domain
WD40_Gbeta PF25391 720 - 859 8.7e-08 G protein beta WD-40 repeat protein
Beta-prop_WDR36-Utp21_1st PF25171 720 - 854 3.6e-07 WDR36/Utp21 first beta-propeller
Beta-prop_WDR3_1st PF25173 721 - 860 2.1e-16 WDR3 first beta-propeller domain
WD40_CDC20-Fz PF24807 724 - 859 7.1e-09 CDC20/Fizzy WD40 domain
WD40 PF00400 739 - 773 3.2e-08 WD domain, G-beta repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000670)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G32551 AT4G32551
fragaria_vesca FvH4_1g04170 FvH4_1g04170 FvH4_1g04170 FvH4_1g04170 FvH4_1g04170 FvH4_1g04170 FvH4_1g04170 FvH4_1g04170 FvH4_2g29940 FvH4_2g29940 FvH4_2g29940 FvH4_2g29940 FvH4_2g29940 FvH4_2g29940 FvH4_2g29940 FvH4_2g29940
malus_domestica MD02G1040200.v1.1 MD08G1139100.v1.1 MD15G1117100.v1.1 MD15G1145100.v1.1
prunus_persica Prupe.1G468400_v2.0.a1 Prupe.1G468400_v2.0.a1 Prupe.1G468400_v2.0.a1 Prupe.1G468400_v2.0.a1 Prupe.1G468400_v2.0.a1 Prupe.1G468400_v2.0.a1 Prupe.1G468400_v2.0.a1 Prupe.1G468400_v2.0.a1 Prupe.1G468400_v2.0.a1 Prupe.1G468400_v2.0.a1 Prupe.1G468400_v2.0.a1 Prupe.1G468400_v2.0.a1 Prupe.1G468400_v2.0.a1 Prupe.1G468400_v2.0.a1 Prupe.1G468400_v2.0.a1 Prupe.1G468400_v2.0.a1 Prupe.1G468400_v2.0.a1 Prupe.7G235500_v2.0.a1 Prupe.7G235500_v2.0.a1 Prupe.7G235500_v2.0.a1 Prupe.7G235500_v2.0.a1
pyrus_communis pycom02g03350 pycom08g11750 pycom15g10550 pycom15g16250
rosa_chinensis RchiOBHm_Chr2g0089671 RchiOBHm_Chr3g0465211 RchiOBHm_Chr6g0312941
rosa_laevigata RLG00000010283 RLG00000016050
rosa_multiflora Rmu_sc0000665.1_g000023 Rmu_sc0008049.1_g000043 Rmu_sc0008049.1_g000044
rosa_roxburghii Rroxscaffold_2G00151660 Rroxscaffold_7G00156310
rosa_rugosa Rorug02G0002500 Rorug02G0002500 Rorug06G0400700 Rorug06G0400700 Rorug06G0400700 Rorug06G0400700 Rorug06G0400800.1 Rorug06G0400900.1
rosa_samantha Rh2AG047900 Rh2BG046500 Rh2CG048500 Rh2DG047900 Rh6AG517700 Rh6BG529400 Rh6CG533900 Rh6DG520500
rosa_wichuraiana Rw2G004260 Rw5G017770 Rw6G045010

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 632
AasI GACNNNNNNGTC 2 cut(s) 1934, 2564
Acc36I ACCTGC 1 cut(s) 632
AccB1I GGYRCC 1 cut(s) 1294
AccI GTMKAC 2 cut(s) 1590, 1847
AciI CCGC 3 cut(s) 1015, 1217, 2516
AclI AACGTT 1 cut(s) 1626
AcoI YGGCCR 1 cut(s) 646
AcsI RAATTY 6 cut(s) 490, 904, 976, 1240, 2345, 2378
AcuI CTGAAG 5 cut(s) 878, 1241, 1687, 1752, 2529
AcvI CACGTG 1 cut(s) 1559
AcyI GRCGYC 1 cut(s) 1295
AdeI CACNNNGTG 1 cut(s) 152
AfaI GTAC 6 cut(s) 512, 676, 1086, 1738, 1978, 2075
AfeI AGCGCT 1 cut(s) 2245
AfiI CCNNNNNNNGG 7 cut(s) 863, 1094, 1223, 1326, 1505, 1794, 2150
AflII CTTAAG 1 cut(s) 2222
AflIII ACRYGT 1 cut(s) 2387
AgsI TTSAA 9 cut(s) 104, 704, 1245, 1344, 1449, 1751, 2113, 2141, 2383
AjnI CCWGG 5 cut(s) 148, 655, 679, 733, 1960
AloI GAACNNNNNNTCC 2 cut(s) 2311, 2343
Alw21I GWGCWC 1 cut(s) 1826
Alw26I GTCTC 5 cut(s) 9, 239, 1527, 2198, 2432
Alw44I GTGCAC 1 cut(s) 1822
Aor51HI AGCGCT 1 cut(s) 2245
AoxI GGCC 2 cut(s) 646, 854
ApaLI GTGCAC 1 cut(s) 1822
ApoI RAATTY 6 cut(s) 490, 904, 976, 1240, 2345, 2378
Asp700I GAANNNNTTC 2 cut(s) 118, 2349
AspLEI GCGC 5 cut(s) 809, 1108, 1297, 1570, 2246
AspS9I GGNCC 3 cut(s) 429, 1391, 1427
AsuC2I CCSGG 1 cut(s) 1121
AsuHPI GGTGA 4 cut(s) 775, 1092, 1434, 2143
AsuII TTCGAA 1 cut(s) 165
AsuNHI GCTAGC 1 cut(s) 964
AvaII GGWCC 3 cut(s) 429, 1391, 1427
BaeGI GKGCMC 1 cut(s) 1826
BaeI ACNNNNGTAYC 4 cut(s) 2065, 2065, 2098, 2098
BalI TGGCCA 1 cut(s) 648
BamHI GGATCC 3 cut(s) 127, 604, 2266
BanI GGYRCC 1 cut(s) 1294
BanII GRGCYC 1 cut(s) 1556
BauI CACGAG 2 cut(s) 1131, 2354
BbrPI CACGTG 1 cut(s) 1559
BbsI GAAGAC 1 cut(s) 2499
Bbv12I GWGCWC 1 cut(s) 1826
BbvCI CCTCAGC 1 cut(s) 291
BcgI CGANNNNNNTGC 2 cut(s) 401, 435
BciT130I CCWGG 5 cut(s) 150, 657, 681, 735, 1962
BciVI GTATCC 1 cut(s) 2197
BclI TGATCA 1 cut(s) 1333
BcnI CCSGG 1 cut(s) 1121
BcoDI GTCTC 5 cut(s) 9, 239, 1527, 2198, 2432
BfaI CTAG 6 cut(s) 201, 965, 1662, 1896, 2285, 2501
BfmI CTRYAG 3 cut(s) 132, 549, 2169
BfoI RGCGCY 4 cut(s) 810, 1298, 1571, 2247
BfrI CTTAAG 1 cut(s) 2222
BfuAI ACCTGC 1 cut(s) 632
BfuI GTATCC 1 cut(s) 2197
BlpI GCTNAGC 1 cut(s) 342
Bme1390I CCNGG 6 cut(s) 150, 657, 681, 735, 1121, 1962
Bme18I GGWCC 3 cut(s) 429, 1391, 1427
BmgT120I GGNCC 3 cut(s) 429, 1391, 1427
BmiI GGNNCC 8 cut(s) 129, 430, 431, 606, 1296, 1392, 2268, 2329
BmrFI CCNGG 6 cut(s) 150, 657, 681, 735, 1121, 1962
BmrI ACTGGG 2 cut(s) 1431, 2541
BmtI GCTAGC 1 cut(s) 968
BmuI ACTGGG 2 cut(s) 1431, 2541
BpiI GAAGAC 1 cut(s) 2499
BplI GAGNNNNNCTC 2 cut(s) 203, 235
BpmI CTGGAG 4 cut(s) 702, 771, 2098, 2124
Bpu10I CCTNAGC 2 cut(s) 291, 1405
Bpu1102I GCTNAGC 1 cut(s) 342
Bpu14I TTCGAA 1 cut(s) 165
BpuEI CTTGAG 1 cut(s) 234
BpuMI CCSGG 1 cut(s) 1121
BsaAI YACGTR 3 cut(s) 1559, 1976, 2388
BsaBI GATNNNNATC 1 cut(s) 609
BsaHI GRCGYC 1 cut(s) 1295
BsaJI CCNNGG 2 cut(s) 123, 1960
Bsc4I CCNNNNNNNGG 7 cut(s) 863, 1094, 1223, 1326, 1505, 1794, 2150
Bse1I ACTGG 5 cut(s) 1376, 1437, 1795, 2081, 2536
Bse3DI GCAATG 1 cut(s) 195
Bse8I GATNNNNATC 1 cut(s) 609
BseBI CCWGG 5 cut(s) 150, 657, 681, 735, 1962
BseDI CCNNGG 2 cut(s) 123, 1960
BseGI GGATG 8 cut(s) 49, 857, 1023, 1066, 1213, 1954, 2203, 2398
BseJI GATNNNNATC 1 cut(s) 609
BseLI CCNNNNNNNGG 7 cut(s) 863, 1094, 1223, 1326, 1505, 1794, 2150
BseMI GCAATG 1 cut(s) 195
BseMII CTCAG 7 cut(s) 20, 234, 305, 356, 1250, 1419, 2141
BseNI ACTGG 5 cut(s) 1376, 1437, 1795, 2081, 2536
BseRI GAGGAG 1 cut(s) 530
BseSI GKGCMC 1 cut(s) 1826
BseYI CCCAGC 3 cut(s) 432, 2024, 2262
BsgI GTGCAG 1 cut(s) 2468
BshFI GGCC 2 cut(s) 648, 856
BshNI GGYRCC 1 cut(s) 1294
BsiHKAI GWGCWC 1 cut(s) 1826
BsiSI CCGG 1 cut(s) 1121
BslFI GGGAC 5 cut(s) 200, 442, 904, 1423, 2001
BslI CCNNNNNNNGG 7 cut(s) 863, 1094, 1223, 1326, 1505, 1794, 2150
BsmAI GTCTC 5 cut(s) 9, 239, 1527, 2198, 2432
BsmFI GGGAC 5 cut(s) 200, 442, 904, 1423, 2001
BsmI GAATGC 2 cut(s) 1231, 1861
BsnI GGCC 2 cut(s) 648, 856
Bsp119I TTCGAA 1 cut(s) 165
Bsp1286I GDGCHC 2 cut(s) 1556, 1826
Bsp1720I GCTNAGC 1 cut(s) 342
BspACI CCGC 3 cut(s) 1015, 1217, 2516
BspANI GGCC 2 cut(s) 648, 856
BspCNI CTCAG 7 cut(s) 19, 233, 304, 355, 1249, 1418, 2140
BspLI GGNNCC 8 cut(s) 129, 430, 431, 606, 1296, 1392, 2268, 2329
BspMAI CTGCAG 1 cut(s) 2173
BspMI ACCTGC 1 cut(s) 632
BspOI GCTAGC 1 cut(s) 968
BspT104I TTCGAA 1 cut(s) 165
BspT107I GGYRCC 1 cut(s) 1294
BspTI CTTAAG 1 cut(s) 2222
BsrDI GCAATG 1 cut(s) 195
BsrI ACTGG 5 cut(s) 1376, 1437, 1795, 2081, 2536
BssECI CCNNGG 2 cut(s) 123, 1960
BssNI GRCGYC 1 cut(s) 1295
BssSI CACGAG 2 cut(s) 1131, 2354
Bst2BI CACGAG 2 cut(s) 1131, 2354
Bst2UI CCWGG 5 cut(s) 150, 657, 681, 735, 1962
Bst4CI ACNGT 9 cut(s) 716, 1414, 1595, 1936, 2002, 2063, 2073, 2093, 2145
Bst6I CTCTTC 1 cut(s) 65
BstACI GRCGYC 1 cut(s) 1295
BstAFI CTTAAG 1 cut(s) 2222
BstAPI GCANNNNNTGC 1 cut(s) 961
BstBAI YACGTR 3 cut(s) 1559, 1976, 2388
BstBI TTCGAA 1 cut(s) 165
BstC8I GCNNGC 8 cut(s) 339, 650, 966, 1310, 1911, 2219, 2487, 2551
BstDEI CTNAG 7 cut(s) 6, 220, 291, 342, 1236, 1405, 2127
BstDSI CCRYGG 1 cut(s) 123
BstF5I GGATG 8 cut(s) 49, 857, 1023, 1066, 1213, 1954, 2203, 2398
BstH2I RGCGCY 4 cut(s) 810, 1298, 1571, 2247
BstHHI GCGC 5 cut(s) 809, 1108, 1297, 1570, 2246
BstMAI GTCTC 5 cut(s) 9, 239, 1527, 2198, 2432
BstNI CCWGG 5 cut(s) 150, 657, 681, 735, 1962
BstNSI RCATGY 5 cut(s) 86, 341, 1150, 1312, 1903
BstSCI CCNGG 6 cut(s) 148, 655, 679, 733, 1119, 1960
BstSFI CTRYAG 3 cut(s) 132, 549, 2169
BstSLI GKGCMC 1 cut(s) 1826
BstSNI TACGTA 1 cut(s) 1976
BstV2I GAAGAC 1 cut(s) 2499
BstX2I RGATCY 4 cut(s) 127, 604, 1610, 2266
BstXI CCANNNNNNTGG 3 cut(s) 656, 1003, 1508
BstYI RGATCY 4 cut(s) 127, 604, 1610, 2266
BsuI GTATCC 1 cut(s) 2197
BsuRI GGCC 2 cut(s) 648, 856
BtgI CCRYGG 1 cut(s) 123
BtsCI GGATG 8 cut(s) 49, 857, 1023, 1066, 1213, 1954, 2203, 2398
BtsI GCAGTG 1 cut(s) 447
BtsIMutI CAGTG 7 cut(s) 447, 1383, 1788, 1824, 1998, 2068, 2298
BveI ACCTGC 1 cut(s) 632
Cac8I GCNNGC 8 cut(s) 339, 650, 966, 1310, 1911, 2219, 2487, 2551
CfoI GCGC 5 cut(s) 809, 1108, 1297, 1570, 2246
Cfr13I GGNCC 3 cut(s) 429, 1391, 1427
CseI GACGC 3 cut(s) 407, 606, 2554
CsiI ACCWGGT 1 cut(s) 148
Csp6I GTAC 6 cut(s) 511, 675, 1085, 1737, 1977, 2074
CviQI GTAC 6 cut(s) 511, 675, 1085, 1737, 1977, 2074
DdeI CTNAG 7 cut(s) 6, 220, 291, 342, 1236, 1405, 2127
DinI GGCGCC 1 cut(s) 1296
DraIII CACNNNGTG 1 cut(s) 152
DrdI GACNNNNNNGTC 2 cut(s) 1934, 2564
DseDI GACNNNNNNGTC 2 cut(s) 1934, 2564
EaeI YGGCCR 1 cut(s) 646
Eam1104I CTCTTC 1 cut(s) 65
EarI CTCTTC 1 cut(s) 65
EciI GGCGGA 1 cut(s) 1030
Eco105I TACGTA 1 cut(s) 1976
Eco24I GRGCYC 1 cut(s) 1556
Eco47I GGWCC 3 cut(s) 429, 1391, 1427
Eco47III AGCGCT 1 cut(s) 2245
Eco57I CTGAAG 5 cut(s) 878, 1241, 1687, 1752, 2529
Eco72I CACGTG 1 cut(s) 1559
EcoO109I RGGNCCY 2 cut(s) 429, 1391
EcoRI GAATTC 2 cut(s) 1240, 2345
EcoRII CCWGG 5 cut(s) 148, 655, 679, 733, 1960
EcoT38I GRGCYC 1 cut(s) 1556
EgeI GGCGCC 1 cut(s) 1296
EheI GGCGCC 1 cut(s) 1296
FalI AAGNNNNNCTT 2 cut(s) 1707, 1739
FaqI GGGAC 5 cut(s) 200, 442, 904, 1423, 2001
FbaI TGATCA 1 cut(s) 1333
FblI GTMKAC 2 cut(s) 1590, 1847
FokI GGATG 8 cut(s) 56, 864, 1030, 1073, 1200, 1961, 2210, 2385
FriOI GRGCYC 1 cut(s) 1556
FspBI CTAG 6 cut(s) 201, 965, 1662, 1896, 2285, 2501
GlaI GCGC 5 cut(s) 808, 1107, 1296, 1569, 2245
GsaI CCCAGC 3 cut(s) 436, 2028, 2266
GsuI CTGGAG 4 cut(s) 702, 771, 2098, 2124
HaeII RGCGCY 4 cut(s) 810, 1298, 1571, 2247
HaeIII GGCC 2 cut(s) 648, 856
HapII CCGG 1 cut(s) 1121
HgaI GACGC 3 cut(s) 407, 606, 2554
HhaI GCGC 5 cut(s) 809, 1108, 1297, 1570, 2246
Hin1I GRCGYC 1 cut(s) 1295
Hin6I GCGC 5 cut(s) 807, 1106, 1295, 1568, 2244
HinP1I GCGC 5 cut(s) 807, 1106, 1295, 1568, 2244
HincII GTYRAC 1 cut(s) 1603
HindII GTYRAC 1 cut(s) 1603
HindIII AAGCTT 1 cut(s) 527
HpaII CCGG 1 cut(s) 1121
HphI GGTGA 4 cut(s) 775, 1092, 1434, 2143
Hpy166II GTNNAC 8 cut(s) 677, 1442, 1591, 1603, 1824, 1848, 2014, 2319
Hpy188I TCNGA 9 cut(s) 9, 223, 720, 846, 1239, 1543, 1687, 1771, 2403
Hpy188III TCNNGA 8 cut(s) 104, 422, 788, 2086, 2203, 2354, 2492, 2575
Hpy8I GTNNAC 8 cut(s) 677, 1442, 1591, 1603, 1824, 1848, 2014, 2319
Hpy99I CGWCG 3 cut(s) 423, 622, 1519
HpyAV CCTTC 7 cut(s) 104, 785, 1051, 1265, 1455, 1464, 2280
HpyCH4III ACNGT 9 cut(s) 716, 1414, 1595, 1936, 2002, 2063, 2073, 2093, 2145
HpyCH4IV ACGT 5 cut(s) 1558, 1626, 1881, 1975, 2387
HpyF3I CTNAG 7 cut(s) 6, 220, 291, 342, 1236, 1405, 2127
HpySE526I ACGT 5 cut(s) 1558, 1626, 1881, 1975, 2387
Hsp92I GRCGYC 1 cut(s) 1295
HspAI GCGC 5 cut(s) 807, 1106, 1295, 1568, 2244
KasI GGCGCC 1 cut(s) 1294
KflI GGGWCCC 1 cut(s) 429
Ksp22I TGATCA 1 cut(s) 1333
LmnI GCTCC 4 cut(s) 268, 790, 2051, 2446
MabI ACCWGGT 1 cut(s) 148
MaeI CTAG 6 cut(s) 201, 965, 1662, 1896, 2285, 2501
MaeII ACGT 5 cut(s) 1558, 1626, 1881, 1975, 2387
MaeIII GTNAC 3 cut(s) 1158, 2226, 2297
MfeI CAATTG 2 cut(s) 19, 2322
MflI RGATCY 4 cut(s) 127, 604, 1610, 2266
MhlI GDGCHC 2 cut(s) 1556, 1826
MlsI TGGCCA 1 cut(s) 648
MluNI TGGCCA 1 cut(s) 648
Mly113I GGCGCC 1 cut(s) 1295
MlyI GAGTC 7 cut(s) 241, 601, 730, 835, 1640, 1680, 2473
MmeI TCCRAC 4 cut(s) 21, 698, 1075, 2426
Mox20I TGGCCA 1 cut(s) 648
MroXI GAANNNNTTC 2 cut(s) 118, 2349
MscI TGGCCA 1 cut(s) 648
MseI TTAA 6 cut(s) 524, 860, 1070, 1416, 1710, 2223
MslI CAYNNNNRTG 6 cut(s) 1151, 1484, 1506, 1650, 2003, 2009
Msp20I TGGCCA 1 cut(s) 648
MspA1I CMGCKG 3 cut(s) 230, 295, 2334
MspCI CTTAAG 1 cut(s) 2222
MspI CCGG 1 cut(s) 1121
MspR9I CCNGG 6 cut(s) 150, 657, 681, 735, 1121, 1962
MunI CAATTG 2 cut(s) 19, 2322
Mva1269I GAATGC 2 cut(s) 1231, 1861
MvaI CCWGG 5 cut(s) 150, 657, 681, 735, 1962
NarI GGCGCC 1 cut(s) 1295
NciI CCSGG 1 cut(s) 1121
NheI GCTAGC 1 cut(s) 964
NlaIV GGNNCC 8 cut(s) 129, 430, 431, 606, 1296, 1392, 2268, 2329
NmuCI GTSAC 1 cut(s) 2297
NspI RCATGY 5 cut(s) 86, 341, 1150, 1312, 1903
NspV TTCGAA 1 cut(s) 165
PaeI GCATGC 2 cut(s) 341, 1312
PaqCI CACCTGC 1 cut(s) 632
PctI GAATGC 2 cut(s) 1231, 1861
PdmI GAANNNNTTC 2 cut(s) 118, 2349
PfeI GAWTC 4 cut(s) 458, 697, 889, 1348
PleI GAGTC 7 cut(s) 241, 600, 729, 835, 1639, 1679, 2473
PluTI GGCGCC 1 cut(s) 1298
PmaCI CACGTG 1 cut(s) 1559
PmlI CACGTG 1 cut(s) 1559
PpsI GAGTC 7 cut(s) 241, 600, 729, 835, 1639, 1679, 2473
Ppu21I YACGTR 3 cut(s) 1559, 1976, 2388
PpuMI RGGWCCY 2 cut(s) 429, 1391
Psp1406I AACGTT 1 cut(s) 1626
Psp5II RGGWCCY 2 cut(s) 429, 1391
Psp6I CCWGG 5 cut(s) 148, 655, 679, 733, 1960
PspCI CACGTG 1 cut(s) 1559
PspFI CCCAGC 3 cut(s) 432, 2024, 2262
PspGI CCWGG 5 cut(s) 148, 655, 679, 733, 1960
PspN4I GGNNCC 8 cut(s) 129, 430, 431, 606, 1296, 1392, 2268, 2329
PspPI GGNCC 3 cut(s) 429, 1391, 1427
PspPPI RGGWCCY 2 cut(s) 429, 1391
PstI CTGCAG 1 cut(s) 2173
PsuI RGATCY 4 cut(s) 127, 604, 1610, 2266
PvuII CAGCTG 3 cut(s) 230, 295, 2334
RsaI GTAC 6 cut(s) 512, 676, 1086, 1738, 1978, 2075
RsaNI GTAC 6 cut(s) 511, 675, 1085, 1737, 1977, 2074
RseI CAYNNNNRTG 6 cut(s) 1151, 1484, 1506, 1650, 2003, 2009
SaqAI TTAA 6 cut(s) 524, 860, 1070, 1416, 1710, 2223
Sau96I GGNCC 3 cut(s) 429, 1391, 1427
SchI GAGTC 7 cut(s) 241, 601, 730, 835, 1640, 1680, 2473
ScrFI CCNGG 6 cut(s) 150, 657, 681, 735, 1121, 1962
SduI GDGCHC 2 cut(s) 1556, 1826
SexAI ACCWGGT 1 cut(s) 148
SfcI CTRYAG 3 cut(s) 132, 549, 2169
SfoI GGCGCC 1 cut(s) 1296
SfuI TTCGAA 1 cut(s) 165
SinI GGWCC 3 cut(s) 429, 1391, 1427
SmiMI CAYNNNNRTG 6 cut(s) 1151, 1484, 1506, 1650, 2003, 2009
SmlI CTYRAG 2 cut(s) 249, 2222
SmoI CTYRAG 2 cut(s) 249, 2222
SnaBI TACGTA 1 cut(s) 1976
SphI GCATGC 2 cut(s) 341, 1312
SsiI CCGC 3 cut(s) 1015, 1217, 2516
SspDI GGCGCC 1 cut(s) 1294
SspMI CTAG 6 cut(s) 201, 965, 1662, 1896, 2285, 2501
StyD4I CCNGG 6 cut(s) 148, 655, 679, 733, 1119, 1960
TaaI ACNGT 9 cut(s) 716, 1414, 1595, 1936, 2002, 2063, 2073, 2093, 2145
TaiI ACGT 5 cut(s) 1561, 1629, 1884, 1978, 2390
TaqI TCGA 9 cut(s) 165, 421, 620, 880, 1039, 1080, 1517, 2202, 2311
TaqII GACCGA 2 cut(s) 1818, 2477
TatI WGTACW 1 cut(s) 1736
TfiI GAWTC 4 cut(s) 458, 697, 889, 1348
Tru1I TTAA 6 cut(s) 524, 860, 1070, 1416, 1710, 2223
Tru9I TTAA 6 cut(s) 524, 860, 1070, 1416, 1710, 2223
TscAI CASTG 7 cut(s) 454, 1383, 1795, 1831, 2005, 2068, 2298
TseFI GTSAC 1 cut(s) 2297
Tsp45I GTSAC 1 cut(s) 2297
TspGWI ACGGA 1 cut(s) 140
TspRI CASTG 7 cut(s) 454, 1383, 1795, 1831, 2005, 2068, 2298
Vha464I CTTAAG 1 cut(s) 2222
VneI GTGCAC 1 cut(s) 1822
VpaK11BI GGWCC 3 cut(s) 429, 1391, 1427
XapI RAATTY 6 cut(s) 490, 904, 976, 1240, 2345, 2378
XceI RCATGY 5 cut(s) 86, 341, 1150, 1312, 1903
XcmI CCANNNNNNNNNTGG 1 cut(s) 440
XmiI GTMKAC 2 cut(s) 1590, 1847
XmnI GAANNNNTTC 2 cut(s) 118, 2349
XspI CTAG 6 cut(s) 201, 965, 1662, 1896, 2285, 2501
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.