Rh2CG048500

Transcriptional corepressor LEUNIG-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2C
OG0000670
Physical Location & Seq
Forward (+)
3857441 .. 3866596
9156 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2CG048500.1

Sequence Viewer

Length: 2568 bp
ATGGCTCAGGCCAATTGGGAAGCTGATAAAATGTTGGATGTGTATATATATGATTACCTTCTGAAGAGAAATTTACATGCTTCTGCAAAGGCATTTCAAGCTGAAGGAAAAGTTTCTACAGATCCTGTAGCTATTGATGCACCCGGTGGCTTTCTTTTCGAATGGTGGTCTGTCTTCTGGGACATATTCATCGCTAGGACGAATGAAAAGCACTCTGAAGCAGCTGCATCTTATATTGAGACTCAAGTGAACAAGGCTCGGGAGCTGCAACAGAAGCCTCAGCAGCATCCTCAAATGCAAATGCAGCATCTTTTGCAAAGGCATGCTGCTCAGCAGCAGCAGCAGCATCAACAACAGCAACAACAACAACAACAACACCAGCAACAACAACACCCGCAACACCAGCAGCAGCAGCAGCAGCAAAGACGAGATGGGACCCAACTTCTTAATGGAACTTCCAATGGGCTTGTTGGCACTGATCCTCTTTCGAGGCAGAACTCTGCAACTGCAAATGTCATGGCAACAAAAATGTACGAGGACAGATTAAAGCCTCCCATACAGAGGGATGCTTTGGATGATGCGGCTATGAAGCAAAGGTTAGGTGACAATATGAATCAGCTTATGGATTCAAATCGTGCATCATTGGTGAAAGTAGCCACAGCAGGTGGCCAGTCTCCCGGTCAAATGCTGCATGGTACACCTGGAGGTATGTTGGGGAATCTTCAACAAGCTCACAATCGGAGTCAGCAACTTCCTGGATTGATGCAGGACATAAAGAGTGAGATGATGAACCCCAGAGCTGCTGGTCCAGAAGGATCATTGATTGGTCTTCATGGATCAAATGAAGGCAGTAGCAATTTGACTCTGAAGGGGTGGCCTTTAACGGTTCGACCTGGAATTCTTCAGCAGCAAAATTCCATGATGCAGTCCTCTCAGCCCTTTAATCAGCTTCAGCTCCAGCAGCAACTTATACTTCAGGCACAACAAAATTTAGCTTCCCCATCTACCAATGACTTGGAAACTAGAAGGCTATCGATGCTCCTCAATAGAAATATACCTAACGTTGATGTACCTAACGTTGGATCGCCTGTTCAAGTGGGTTGCCCTGTAATGCCTGGTGCAGATGCAGATATGCTTATGAAGCAACAGCAGCTGCAAAGCAACAATCAACACCAACAACAGCAACAGTATTCACAGCATCCATTTTCAAGTCAACACCCTCAGAGTTCGAATCAACACATCCAGCAGCAAGAAAAGATCACTGGTGCTGGCAGCTTTACAGCTGGTGGTAGCATGTCTAACTCCTTTCAAGGCAATGATCAGGCTCCAAAGAATCAAATGGGGCGAAAGAGGAAGCAGCCAGTGTCATCTTCAGGTCCTGCCAATAGTTCAGGGACTGCTAATACCACTGGACCATCCCCCAGTTCACCTTCAACGCCTTCTACTCACACGGCAGGAGATGCGAACTCTGTGCCAACTTTGGCCCATAATAGCGGTTCATCGAAGTCTCTGCTTATGTTTGCTTCTGATGGTCTAGGCTCAGTTGCCTCGGTGCCAAATAAATTGAATGATATGGACCGATTTGTGGATGATGGATCTTTAGAGGATAATGTTGAATCATTCTTATCACATGATGATGCTGACCCTAGGGATAGAGTTGCTCGGTGTTCAGATGTCAGCAAAGGCTTCACTTTTTCGGAAGTTGGGGCTATTACTGCAAGTTCAAGTAAAGTCGAGTGCTGTCACTTCTCAGCAGATGGGAAAACACTCGCCACTGGTGGGCATGATCGAAAGGCTGTATTGTGGTCTACAGAGTCCTTCACTGTAAAGTCTACGCTTGAAGAGCATTCTCAGTGGATAACGGATGTTCGATTCAGTCCTAGTATGTCAAGGCTAGCTACATCTTCTGCTGACAAAACTGTCAGGGTCTGGGATGTTGATAATCCTGGCTATTCACTTCGTACTTTTATGGGACATTTGACAACTGTTATGTCACTTGACTTCCACCCTACTAAAGAGGATCTTTTGTGCTCTTGTGATAACAACAGTGAGATAAGATACTGGAGTATCAAGAATGGTAGTTGTATTGGAGTTTCCAAGGGTGGTGCAACTCAGGTGAGGTTTCAACCTCGTCTTGGAAGGATGCTTGCTGCTGCAGGTGATACTCTTGTATCCATAATTGATGTAGAGACCGACAGTTGCACGGTTAAATTACAGGGTCATAAGAACCTTGTCAATTCTGTGTGCTGGGATTCTTCTGGTGAGTATCTAGCCTCTGTGAGTGATGACTCGGTTAGAGTATGGGCAGTCGGCTCCAGTAGCAAAGGCGAATGCCTTTACGAGTTACCGTGTTCTGGCAACAAATTTCAGACATGCGTCTTCCATCCTACTTATCCATCATTGTTGGTAATCGGCTGTTATGAGACATTGGAACTTTGGAACATGAGCGAGAACAAGACAATGACCTTGCATGCACATGACAAGCTAGTATCTTCTTTGGCAGCGTCAAGTTCCACCGGCATGGTAGCTTCAGCTAGCCATGATAAGTTCGTGAAGCTATGGAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0001101 GO:0001558 GO:0001666 GO:0003002 GO:0003006 GO:0005975 GO:0005976 GO:0006355 GO:0006950 GO:0006974 GO:0006979 GO:0007275 GO:0007389 GO:0008150 GO:0008152 GO:0009414 GO:0009415 GO:0009605 GO:0009607 GO:0009611 GO:0009617 GO:0009620 GO:0009624 GO:0009628 GO:0009636 GO:0009719 GO:0009725 GO:0009733 GO:0009791 GO:0009798 GO:0009845 GO:0009888 GO:0009889 GO:0009890 GO:0009892 GO:0009909 GO:0009943 GO:0009944 GO:0009955 GO:0009987 GO:0010033 GO:0010035 GO:0010038 GO:0010073 GO:0010154 GO:0010191 GO:0010214 GO:0010243 GO:0010272 GO:0010393 GO:0010468 GO:0010556 GO:0010558 GO:0010605 GO:0010629 GO:0014070 GO:0019219 GO:0019222 GO:0022414 GO:0022603 GO:0022604 GO:0030307 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0032501 GO:0032502 GO:0032504 GO:0033554 GO:0036293 GO:0040008 GO:0042221 GO:0042493 GO:0043170 GO:0043207 GO:0044237 GO:0044238 GO:0045892 GO:0045927 GO:0045934 GO:0045995 GO:0046677 GO:0046898 GO:0048316 GO:0048359 GO:0048507 GO:0048509 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048580 GO:0048608 GO:0048609 GO:0048638 GO:0048639 GO:0048731 GO:0048831 GO:0048856 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051094 GO:0051128 GO:0051130 GO:0051171 GO:0051172 GO:0051239 GO:0051252 GO:0051253 GO:0051510 GO:0051512 GO:0051704 GO:0051707 GO:0051716 GO:0060255 GO:0060992 GO:0061458 GO:0065001 GO:0065007 GO:0070482 GO:0071216 GO:0071217 GO:0071496 GO:0071704 GO:0080001 GO:0080090 GO:0090351 GO:0097305 GO:1901654 GO:1901698 GO:1901700 GO:1902074 GO:1902183 GO:1902679 GO:1903506 GO:1903507 GO:2000024 GO:2000026 GO:2000112 GO:2000113 GO:2000241 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LisH WD40

Protein Analysis

855

Amino Acids

93.68

Weight (kDa)

6.49

Isoelectric Point (pI)

51.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LisH PF08513 10 - 36 2.4e-07 LisH
WD40_Gbeta PF25391 562 - 698 3.4e-11 G protein beta WD-40 repeat protein
WD40_MABP1-WDR62_2nd PF24782 564 - 768 1.6e-16 MABP1/WDR62 second WD40 domain
Beta-prop_THOC3 PF25174 564 - 627 6.9e-09 THOC3 beta-propeller domain
EIF3I PF24805 566 - 648 1.1e-06 EIF3I
Beta-prop_TEP1_2nd PF25047 566 - 689 5.5e-13 TEP-1 second beta-propeller
WD40_WDHD1_1st PF24817 567 - 648 6.8e-12 WDHD1 first WD40 domain
Beta-prop_WDR3_2nd PF25172 568 - 769 2e-15 WDR3 second beta-propeller domain
WD40_CDC20-Fz PF24807 568 - 697 1.1e-15 CDC20/Fizzy WD40 domain
Beta-prop_EML_2 PF23414 571 - 694 3.6e-18 Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_WDR5 PF25175 573 - 697 9.8e-26 WDR5 beta-propeller domain
Beta-prop_WDR3_1st PF25173 573 - 690 3.3e-22 WDR3 first beta-propeller domain
WD40_Prp19 PF24814 574 - 770 8.6e-32 Prp19 WD40 domain
Beta-prop_WDR36-Utp21_2nd PF25168 575 - 688 1.4e-07 WDR36/Utp21 second beta-propeller domain
Beta-prop_Aladin PF25460 576 - 770 2.6e-06 Aladin seven-bladed propeller
Beta-prop_EML PF23409 576 - 695 2.2e-06 Echinoderm microtubule-associated protein first beta-propeller
Beta-prop_CAF1B_HIR1 PF24105 582 - 649 2.9e-07 CAF1B/HIR1 beta-propeller domain
WD40 PF00400 610 - 645 3.3e-09 WD domain, G-beta repeat
WDR55 PF24796 610 - 855 1.9e-11 WDR55
Beta-prop_THOC3 PF25174 616 - 855 8.3e-38 THOC3 beta-propeller domain
Beta-prop_WDR90_POC16_2nd PF23393 627 - 736 1.1e-06 WDR90/POC16, second beta-propeller
EIF3I PF24805 658 - 854 2.3e-07 EIF3I
Beta-prop_WDR36-Utp21_2nd PF25168 676 - 774 7.6e-08 WDR36/Utp21 second beta-propeller domain
Beta-prop_WDR3_1st PF25173 682 - 773 6.9e-12 WDR3 first beta-propeller domain
Beta-prop_WDR5 PF25175 701 - 854 4e-25 WDR5 beta-propeller domain
WD40_WDHD1_1st PF24817 703 - 855 1.8e-14 WDHD1 first WD40 domain
WD40_Prp19 PF24814 704 - 854 4.2e-15 Prp19 WD40 domain
WD40_Gbeta PF25391 707 - 854 1.4e-06 G protein beta WD-40 repeat protein
WD40_CDC20-Fz PF24807 719 - 854 1.9e-08 CDC20/Fizzy WD40 domain
WD40 PF00400 733 - 768 1.4e-07 WD domain, G-beta repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0000670)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G32551 AT4G32551
fragaria_vesca FvH4_1g04170 FvH4_1g04170 FvH4_1g04170 FvH4_1g04170 FvH4_1g04170 FvH4_1g04170 FvH4_1g04170 FvH4_1g04170 FvH4_2g29940 FvH4_2g29940 FvH4_2g29940 FvH4_2g29940 FvH4_2g29940 FvH4_2g29940 FvH4_2g29940 FvH4_2g29940
malus_domestica MD02G1040200.v1.1 MD08G1139100.v1.1 MD15G1117100.v1.1 MD15G1145100.v1.1
prunus_persica Prupe.1G468400_v2.0.a1 Prupe.1G468400_v2.0.a1 Prupe.1G468400_v2.0.a1 Prupe.1G468400_v2.0.a1 Prupe.1G468400_v2.0.a1 Prupe.1G468400_v2.0.a1 Prupe.1G468400_v2.0.a1 Prupe.1G468400_v2.0.a1 Prupe.1G468400_v2.0.a1 Prupe.1G468400_v2.0.a1 Prupe.1G468400_v2.0.a1 Prupe.1G468400_v2.0.a1 Prupe.1G468400_v2.0.a1 Prupe.1G468400_v2.0.a1 Prupe.1G468400_v2.0.a1 Prupe.1G468400_v2.0.a1 Prupe.1G468400_v2.0.a1 Prupe.7G235500_v2.0.a1 Prupe.7G235500_v2.0.a1 Prupe.7G235500_v2.0.a1 Prupe.7G235500_v2.0.a1
pyrus_communis pycom02g03350 pycom08g11750 pycom15g10550 pycom15g16250
rosa_chinensis RchiOBHm_Chr2g0089671 RchiOBHm_Chr3g0465211 RchiOBHm_Chr6g0312941
rosa_laevigata RLG00000010283 RLG00000016050
rosa_multiflora Rmu_sc0000665.1_g000023 Rmu_sc0008049.1_g000043 Rmu_sc0008049.1_g000044
rosa_roxburghii Rroxscaffold_2G00151660 Rroxscaffold_7G00156310
rosa_rugosa Rorug02G0002500 Rorug02G0002500 Rorug06G0400700 Rorug06G0400700 Rorug06G0400700 Rorug06G0400700 Rorug06G0400800.1 Rorug06G0400900.1
rosa_samantha Rh2AG047900 Rh2BG046500 Rh2CG048500 Rh2DG047900 Rh6AG517700 Rh6BG529400 Rh6CG533900 Rh6DG520500
rosa_wichuraiana Rw2G004260 Rw5G017770 Rw6G045010

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 653, 2147
AasI GACNNNNNNGTC 1 cut(s) 1919
Acc36I ACCTGC 2 cut(s) 653, 2147
AccB1I GGYRCC 1 cut(s) 1552
AccI GTMKAC 2 cut(s) 1808, 1832
AciI CCGC 3 cut(s) 395, 581, 1494
AclI AACGTT 2 cut(s) 1062, 1077
AclWI GGATC 7 cut(s) 116, 473, 823, 844, 1090, 1603, 2028
AcoI YGGCCR 1 cut(s) 667
AcsI RAATTY 5 cut(s) 70, 897, 913, 988, 2363
AcuI CTGAAG 9 cut(s) 83, 123, 237, 887, 887, 935, 959, 1356, 2514
AdeI CACNNNGTG 1 cut(s) 146
AfaI GTAC 4 cut(s) 533, 697, 1071, 1963
AfiI CCNNNNNNNGG 6 cut(s) 561, 1079, 1585, 1779, 2135, 2354
AjnI CCWGG 5 cut(s) 700, 754, 892, 1114, 1945
AloI GAACNNNNNNTCC 2 cut(s) 1074, 1106
Alw21I GWGCWC 1 cut(s) 2033
Alw26I GTCTC 5 cut(s) 233, 678, 1512, 2183, 2417
AlwI GGATC 7 cut(s) 116, 473, 823, 844, 1090, 1603, 2028
AlwNI CAGNNNCTG 5 cut(s) 125, 1153, 1379, 1397, 1929
Ama87I CYCGRG 1 cut(s) 258
AoxI GGCC 4 cut(s) 9, 667, 875, 1482
ApoI RAATTY 5 cut(s) 70, 897, 913, 988, 2363
Asp700I GAANNNNTTC 1 cut(s) 112
AspA2I CCTAGG 1 cut(s) 1646
AspS9I GGNCC 6 cut(s) 435, 806, 1376, 1412, 1483, 1576
AsuC2I CCSGG 2 cut(s) 144, 678
AsuHPI GGTGA 6 cut(s) 614, 658, 1419, 2128, 2171, 2273
AsuII TTCGAA 2 cut(s) 159, 1229
AsuNHI GCTAGC 2 cut(s) 1894, 2534
AvaI CYCGRG 1 cut(s) 258
AvaII GGWCC 5 cut(s) 435, 806, 1376, 1412, 1576
AvrII CCTAGG 1 cut(s) 1646
BaeI ACNNNNGTAYC 8 cut(s) 2050, 2050, 2083, 2083, 2154, 2154, 2187, 2187
BalI TGGCCA 1 cut(s) 669
BanI GGYRCC 1 cut(s) 1552
BbsI GAAGAC 3 cut(s) 166, 821, 2371
Bbv12I GWGCWC 1 cut(s) 2033
BbvCI CCTCAGC 1 cut(s) 279
BccI CCATC 8 cut(s) 425, 1009, 1423, 1523, 1586, 1751, 2391, 2404
BceAI ACGGC 1 cut(s) 1467
BcgI CGANNNNNNTGC 4 cut(s) 1453, 1487, 1492, 1526
BciT130I CCWGG 5 cut(s) 702, 756, 894, 1116, 1947
BciVI GTATCC 1 cut(s) 2182
BclI TGATCA 1 cut(s) 1318
BcnI CCSGG 2 cut(s) 144, 678
BcoDI GTCTC 5 cut(s) 233, 678, 1512, 2183, 2417
BfaI CTAG 9 cut(s) 195, 1023, 1535, 1647, 1881, 1895, 2270, 2486, 2535
BfmI CTRYAG 4 cut(s) 117, 126, 1809, 2154
BfuAI ACCTGC 2 cut(s) 653, 2147
BfuI GTATCC 1 cut(s) 2182
BlnI CCTAGG 1 cut(s) 1646
BlpI GCTNAGC 1 cut(s) 330
Bme1390I CCNGG 7 cut(s) 144, 678, 702, 756, 894, 1116, 1947
Bme18I GGWCC 5 cut(s) 435, 806, 1376, 1412, 1576
BmeT110I CYCGRG 1 cut(s) 258
BmgT120I GGNCC 6 cut(s) 435, 806, 1376, 1412, 1483, 1576
BmiI GGNNCC 5 cut(s) 436, 437, 1326, 1554, 2314
BmrFI CCNGG 7 cut(s) 144, 678, 702, 756, 894, 1116, 1947
BmrI ACTGGG 1 cut(s) 1416
BmtI GCTAGC 2 cut(s) 1898, 2538
BmuI ACTGGG 1 cut(s) 1416
BoxI GACNNNNGTC 1 cut(s) 2375
BpiI GAAGAC 3 cut(s) 166, 821, 2371
BpmI CTGGAG 4 cut(s) 723, 941, 2083, 2299
Bpu10I CCTNAGC 2 cut(s) 6, 279
Bpu1102I GCTNAGC 1 cut(s) 330
Bpu14I TTCGAA 2 cut(s) 159, 1229
BpuEI CTTGAG 1 cut(s) 228
BpuMI CCSGG 2 cut(s) 144, 678
Bsa29I ATCGAT 1 cut(s) 1034
BsaBI GATNNNNATC 1 cut(s) 630
BsaI GGTCTC 1 cut(s) 2183
BsaJI CCNNGG 3 cut(s) 1548, 1646, 2097
Bsc4I CCNNNNNNNGG 6 cut(s) 561, 1079, 1585, 1779, 2135, 2354
Bse118I RCCGGY 1 cut(s) 2516
Bse1I ACTGG 8 cut(s) 670, 1267, 1361, 1414, 1422, 1780, 2066, 2316
Bse3DI GCAATG 1 cut(s) 1321
Bse8I GATNNNNATC 1 cut(s) 630
BseBI CCWGG 5 cut(s) 702, 756, 894, 1116, 1947
BseCI ATCGAT 1 cut(s) 1034
BseDI CCNNGG 3 cut(s) 1548, 1646, 2097
BseJI GATNNNNATC 1 cut(s) 630
BseLI CCNNNNNNNGG 6 cut(s) 561, 1079, 1585, 1779, 2135, 2354
BseMI GCAATG 1 cut(s) 1321
BseMII CTCAG 9 cut(s) 20, 293, 344, 947, 1235, 1554, 1764, 1865, 2126
BseNI ACTGG 8 cut(s) 670, 1267, 1361, 1414, 1422, 1780, 2066, 2316
BseRI GAGGAG 1 cut(s) 1031
BseYI CCCAGC 1 cut(s) 2247
BsgI GTGCAG 1 cut(s) 1140
BshFI GGCC 4 cut(s) 11, 669, 877, 1484
BshNI GGYRCC 1 cut(s) 1552
BshVI ATCGAT 1 cut(s) 1034
BsiHKAI GWGCWC 1 cut(s) 2033
BsiHKCI CYCGRG 1 cut(s) 258
BsiSI CCGG 3 cut(s) 144, 678, 2517
BslFI GGGAC 4 cut(s) 194, 448, 1408, 1986
BslI CCNNNNNNNGG 6 cut(s) 561, 1079, 1585, 1779, 2135, 2354
BsmAI GTCTC 5 cut(s) 233, 678, 1512, 2183, 2417
BsmFI GGGAC 4 cut(s) 194, 448, 1408, 1986
BsmI GAATGC 2 cut(s) 1846, 2336
BsnI GGCC 4 cut(s) 11, 669, 877, 1484
Bso31I GGTCTC 1 cut(s) 2183
BsoBI CYCGRG 1 cut(s) 258
Bsp119I TTCGAA 2 cut(s) 159, 1229
Bsp1286I GDGCHC 1 cut(s) 2033
Bsp1720I GCTNAGC 1 cut(s) 330
BspACI CCGC 3 cut(s) 395, 581, 1494
BspANI GGCC 4 cut(s) 11, 669, 877, 1484
BspCNI CTCAG 9 cut(s) 19, 292, 343, 946, 1234, 1553, 1763, 1864, 2125
BspDI ATCGAT 1 cut(s) 1034
BspLI GGNNCC 5 cut(s) 436, 437, 1326, 1554, 2314
BspMAI CTGCAG 1 cut(s) 2158
BspMI ACCTGC 2 cut(s) 653, 2147
BspOI GCTAGC 2 cut(s) 1898, 2538
BspPI GGATC 7 cut(s) 116, 473, 823, 844, 1090, 1603, 2028
BspQI GCTCTTC 1 cut(s) 1836
BspT104I TTCGAA 2 cut(s) 159, 1229
BspT107I GGYRCC 1 cut(s) 1552
BspTNI GGTCTC 1 cut(s) 2183
BsrDI GCAATG 1 cut(s) 1321
BsrFI RCCGGY 1 cut(s) 2516
BsrI ACTGG 8 cut(s) 670, 1267, 1361, 1414, 1422, 1780, 2066, 2316
BssAI RCCGGY 1 cut(s) 2516
BssECI CCNNGG 3 cut(s) 1548, 1646, 2097
BssT1I CCWWGG 2 cut(s) 1646, 2097
Bst2UI CCWGG 5 cut(s) 702, 756, 894, 1116, 1947
Bst4CI ACNGT 9 cut(s) 886, 1188, 1825, 1921, 1987, 2048, 2198, 2206, 2349
Bst6I CTCTTC 2 cut(s) 59, 1836
BstAPI GCANNNNNTGC 2 cut(s) 313, 1460
BstBI TTCGAA 2 cut(s) 159, 1229
BstC8I GCNNGC 6 cut(s) 324, 1270, 1896, 2148, 2472, 2536
BstDEI CTNAG 9 cut(s) 6, 279, 330, 933, 1221, 1540, 1750, 1851, 2112
BstMAI GTCTC 5 cut(s) 233, 678, 1512, 2183, 2417
BstNI CCWGG 5 cut(s) 702, 756, 894, 1116, 1947
BstNSI RCATGY 5 cut(s) 80, 326, 1297, 2376, 2474
BstPAI GACNNNNGTC 1 cut(s) 2375
BstSCI CCNGG 7 cut(s) 142, 676, 700, 754, 892, 1114, 1945
BstSFI CTRYAG 4 cut(s) 117, 126, 1809, 2154
BstV2I GAAGAC 3 cut(s) 166, 821, 2371
BstX2I RGATCY 3 cut(s) 121, 1595, 2020
BstXI CCANNNNNNTGG 2 cut(s) 1015, 2521
BstYI RGATCY 3 cut(s) 121, 1595, 2020
Bsu15I ATCGAT 1 cut(s) 1034
BsuI GTATCC 1 cut(s) 2182
BsuRI GGCC 4 cut(s) 11, 669, 877, 1484
BsuTUI ATCGAT 1 cut(s) 1034
BtgZI GCGATG 1 cut(s) 175
BtsIMutI CAGTG 8 cut(s) 474, 1260, 1368, 1407, 1773, 1821, 1859, 2053
BveI ACCTGC 2 cut(s) 653, 2147
Cac8I GCNNGC 6 cut(s) 324, 1270, 1896, 2148, 2472, 2536
CaiI CAGNNNCTG 5 cut(s) 125, 1153, 1379, 1397, 1929
Cfr10I RCCGGY 1 cut(s) 2516
Cfr13I GGNCC 6 cut(s) 435, 806, 1376, 1412, 1483, 1576
ClaI ATCGAT 1 cut(s) 1034
CseI GACGC 2 cut(s) 2365, 2493
Csp6I GTAC 4 cut(s) 532, 696, 1070, 1962
CviQI GTAC 4 cut(s) 532, 696, 1070, 1962
DdeI CTNAG 9 cut(s) 6, 279, 330, 933, 1221, 1540, 1750, 1851, 2112
DraIII CACNNNGTG 1 cut(s) 146
DrdI GACNNNNNNGTC 1 cut(s) 1919
DseDI GACNNNNNNGTC 1 cut(s) 1919
EaeI YGGCCR 1 cut(s) 667
Eam1104I CTCTTC 2 cut(s) 59, 1836
EarI CTCTTC 2 cut(s) 59, 1836
Eco130I CCWWGG 2 cut(s) 1646, 2097
Eco31I GGTCTC 1 cut(s) 2183
Eco47I GGWCC 5 cut(s) 435, 806, 1376, 1412, 1576
Eco57I CTGAAG 9 cut(s) 83, 123, 237, 887, 887, 935, 959, 1356, 2514
Eco88I CYCGRG 1 cut(s) 258
EcoO109I RGGNCCY 2 cut(s) 435, 1376
EcoRI GAATTC 1 cut(s) 897
EcoRII CCWGG 5 cut(s) 700, 754, 892, 1114, 1945
EcoT14I CCWWGG 2 cut(s) 1646, 2097
ErhI CCWWGG 2 cut(s) 1646, 2097
FalI AAGNNNNNCTT 2 cut(s) 2007, 2039
FaqI GGGAC 4 cut(s) 194, 448, 1408, 1986
FauI CCCGC 1 cut(s) 402
FbaI TGATCA 1 cut(s) 1318
FblI GTMKAC 2 cut(s) 1808, 1832
FspBI CTAG 9 cut(s) 195, 1023, 1535, 1647, 1881, 1895, 2270, 2486, 2535
GsaI CCCAGC 1 cut(s) 2251
GsuI CTGGAG 4 cut(s) 723, 941, 2083, 2299
HaeIII GGCC 4 cut(s) 11, 669, 877, 1484
HapII CCGG 3 cut(s) 144, 678, 2517
HgaI GACGC 2 cut(s) 2365, 2493
HincII GTYRAC 1 cut(s) 1214
HindII GTYRAC 1 cut(s) 1214
HpaII CCGG 3 cut(s) 144, 678, 2517
HphI GGTGA 6 cut(s) 614, 658, 1419, 2128, 2171, 2273
Hpy166II GTNNAC 6 cut(s) 250, 698, 1214, 1427, 1809, 1833
Hpy188I TCNGA 9 cut(s) 63, 217, 741, 867, 1224, 1528, 1672, 1699, 2370
Hpy188III TCNNGA 4 cut(s) 260, 809, 2071, 2551
Hpy8I GTNNAC 6 cut(s) 250, 698, 1214, 1427, 1809, 1833
HpyCH4III ACNGT 9 cut(s) 886, 1188, 1825, 1921, 1987, 2048, 2198, 2206, 2349
HpyCH4IV ACGT 2 cut(s) 1062, 1077
HpyF3I CTNAG 9 cut(s) 6, 279, 330, 933, 1221, 1540, 1750, 1851, 2112
HpySE526I ACGT 2 cut(s) 1062, 1077
KflI GGGWCCC 1 cut(s) 435
Ksp22I TGATCA 1 cut(s) 1318
LguI GCTCTTC 1 cut(s) 1836
LmnI GCTCC 5 cut(s) 262, 960, 1044, 1330, 2318
MaeI CTAG 9 cut(s) 195, 1023, 1535, 1647, 1881, 1895, 2270, 2486, 2535
MaeII ACGT 2 cut(s) 1062, 1077
MaeIII GTNAC 4 cut(s) 602, 1742, 1992, 2343
MfeI CAATTG 1 cut(s) 13
MflI RGATCY 3 cut(s) 121, 1595, 2020
MhlI GDGCHC 1 cut(s) 2033
MlsI TGGCCA 1 cut(s) 669
MluNI TGGCCA 1 cut(s) 669
MlyI GAGTC 5 cut(s) 235, 751, 856, 1823, 2282
MmeI TCCRAC 2 cut(s) 15, 1060
Mox20I TGGCCA 1 cut(s) 669
MroXI GAANNNNTTC 1 cut(s) 112
MscI TGGCCA 1 cut(s) 669
MseI TTAA 5 cut(s) 447, 545, 881, 942, 2208
MslI CAYNNNNRTG 3 cut(s) 1635, 2475, 2519
Msp20I TGGCCA 1 cut(s) 669
MspA1I CMGCKG 3 cut(s) 224, 1153, 1283
MspI CCGG 3 cut(s) 144, 678, 2517
MspR9I CCNGG 7 cut(s) 144, 678, 702, 756, 894, 1116, 1947
MunI CAATTG 1 cut(s) 13
Mva1269I GAATGC 2 cut(s) 1846, 2336
MvaI CCWGG 5 cut(s) 702, 756, 894, 1116, 1947
NciI CCSGG 2 cut(s) 144, 678
NheI GCTAGC 2 cut(s) 1894, 2534
NlaIV GGNNCC 5 cut(s) 436, 437, 1326, 1554, 2314
NmuCI GTSAC 3 cut(s) 602, 1742, 1992
NspI RCATGY 5 cut(s) 80, 326, 1297, 2376, 2474
NspV TTCGAA 2 cut(s) 159, 1229
PaeI GCATGC 2 cut(s) 326, 2474
PaqCI CACCTGC 2 cut(s) 653, 2147
PciSI GCTCTTC 1 cut(s) 1836
PctI GAATGC 2 cut(s) 1846, 2336
PdmI GAANNNNTTC 1 cut(s) 112
PfeI GAWTC 8 cut(s) 613, 626, 718, 1231, 1333, 1616, 1872, 2252
PfoI TCCNGGA 1 cut(s) 754
PleI GAGTC 5 cut(s) 235, 750, 856, 1822, 2282
PpsI GAGTC 5 cut(s) 235, 750, 856, 1822, 2282
PpuMI RGGWCCY 2 cut(s) 435, 1376
PshAI GACNNNNGTC 1 cut(s) 2375
Psp1406I AACGTT 2 cut(s) 1062, 1077
Psp5II RGGWCCY 2 cut(s) 435, 1376
Psp6I CCWGG 5 cut(s) 700, 754, 892, 1114, 1945
PspFI CCCAGC 1 cut(s) 2247
PspGI CCWGG 5 cut(s) 700, 754, 892, 1114, 1945
PspN4I GGNNCC 5 cut(s) 436, 437, 1326, 1554, 2314
PspPI GGNCC 6 cut(s) 435, 806, 1376, 1412, 1483, 1576
PspPPI RGGWCCY 2 cut(s) 435, 1376
PstI CTGCAG 1 cut(s) 2158
PstNI CAGNNNCTG 5 cut(s) 125, 1153, 1379, 1397, 1929
PsuI RGATCY 3 cut(s) 121, 1595, 2020
PvuII CAGCTG 3 cut(s) 224, 1153, 1283
RsaI GTAC 4 cut(s) 533, 697, 1071, 1963
RsaNI GTAC 4 cut(s) 532, 696, 1070, 1962
RseI CAYNNNNRTG 3 cut(s) 1635, 2475, 2519
SapI GCTCTTC 1 cut(s) 1836
SaqAI TTAA 5 cut(s) 447, 545, 881, 942, 2208
Sau96I GGNCC 6 cut(s) 435, 806, 1376, 1412, 1483, 1576
SchI GAGTC 5 cut(s) 235, 751, 856, 1823, 2282
ScrFI CCNGG 7 cut(s) 144, 678, 702, 756, 894, 1116, 1947
SduI GDGCHC 1 cut(s) 2033
SfcI CTRYAG 4 cut(s) 117, 126, 1809, 2154
SfuI TTCGAA 2 cut(s) 159, 1229
SinI GGWCC 5 cut(s) 435, 806, 1376, 1412, 1576
SmiMI CAYNNNNRTG 3 cut(s) 1635, 2475, 2519
SmlI CTYRAG 1 cut(s) 243
SmoI CTYRAG 1 cut(s) 243
SphI GCATGC 2 cut(s) 326, 2474
SsiI CCGC 3 cut(s) 395, 581, 1494
SspMI CTAG 9 cut(s) 195, 1023, 1535, 1647, 1881, 1895, 2270, 2486, 2535
StyD4I CCNGG 7 cut(s) 142, 676, 700, 754, 892, 1114, 1945
StyI CCWWGG 2 cut(s) 1646, 2097
TaaI ACNGT 9 cut(s) 886, 1188, 1825, 1921, 1987, 2048, 2198, 2206, 2349
TaiI ACGT 2 cut(s) 1065, 1080
TaqI TCGA 9 cut(s) 159, 488, 889, 1034, 1229, 1502, 1734, 1789, 1870
TaqII GACCGA 2 cut(s) 1593, 2207
TauI GCSGC 1 cut(s) 584
TfiI GAWTC 8 cut(s) 613, 626, 718, 1231, 1333, 1616, 1872, 2252
Tru1I TTAA 5 cut(s) 447, 545, 881, 942, 2208
Tru9I TTAA 5 cut(s) 447, 545, 881, 942, 2208
TscAI CASTG 8 cut(s) 481, 1267, 1368, 1414, 1780, 1828, 1859, 2053
TseFI GTSAC 3 cut(s) 602, 1742, 1992
Tsp45I GTSAC 3 cut(s) 602, 1742, 1992
TspDTI ATGAA 9 cut(s) 178, 219, 602, 626, 803, 821, 858, 1154, 1488
TspGWI ACGGA 1 cut(s) 1877
TspRI CASTG 8 cut(s) 481, 1267, 1368, 1414, 1780, 1828, 1859, 2053
VpaK11BI GGWCC 5 cut(s) 435, 806, 1376, 1412, 1576
XapI RAATTY 5 cut(s) 70, 897, 913, 988, 2363
XceI RCATGY 5 cut(s) 80, 326, 1297, 2376, 2474
XcmI CCANNNNNNNNNTGG 1 cut(s) 446
XmaJI CCTAGG 1 cut(s) 1646
XmiI GTMKAC 2 cut(s) 1808, 1832
XmnI GAANNNNTTC 1 cut(s) 112
XspI CTAG 9 cut(s) 195, 1023, 1535, 1647, 1881, 1895, 2270, 2486, 2535
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.