Rroxscaffold_2G00151660

Transcriptional corepressor LEUNIG-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0000670
Physical Location & Seq
Reverse (-)
88866192 .. 88874637
8446 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00151660.1

Sequence Viewer

Length: 2190 bp
ATGGCTCAGGCCAATTGGGAAGCTGATAAAATGTTGGATGTGTATATATATGATTACCTTCTGAAGAGAAATTTACATGCTTCTGCAAAGGCATTTCAAGCTGAAGGAAAAGTTTCTACAGATCCTGTAGCTATTGATGCACCCGGTGGCTTTCTTTTCGAATGGTGGTCTGTCTTCTGGGACATATTCATTGCTAGGACGAATGAAAAGCACTCTGAAGCAGCTGCATCTTATATTGAGAACTCTGCAACTGCAAATGTCATGGCAACAAAAATGTACGAGGACAGATTAAAGCCTCCCATACAGAGGGATGCTTTGGATGATGCGACTATGAAGCAAAGGTTAGGTGACAATATGAATCAGCTTATGGATTCAAATCGTGCATCATTGGTGAAAGTAGCCACAGCAGGTGGCCAGTCTCCCGGTCAAATGCTGCATGGTACACCTGGAGGTATGTTGGGGAATCTTCAACAAGCTCACAATCGGAGTCAGCAACTTCCTGGATTGATGCAGGTTCGACCTGGAATTCTTCAGCAGCAAAATTCCATGATGCAGTCCTCTCAGCCCTTTAATCAGCTCCAGCAGCAACTTATACTTCAGGCACAACAAAATTTAGCTTCCCCATCTACCAATGACTTGGAAACTAGAAGGCTATCGATGCTCCTCAATAGAAATATACCTAACGTTGATGTACCTAACGTTGGATCGCCTGTTCAAGTGGGTTGCCCTGTAATGCCTGGTGCAGATGCAGATATGCTTATGAAGTTACAGCAACAGCAGCTGCAAAGCAACAATCAACACCAACAACAGCAACAGTTTTCACAGCATCCATTTTCAAGTCAACACCCTCAGAGTTCGAATCAACACATCCAGCAGCAAGAAAAGATCACTGGTGCTGGCAGCTTTACAGCTGGTGGTAGCATGTCCAACTCCTTTCAAGGCAATGATCAGGCTCCAAAGAATCAAATGGGGCGAAAGAGGAAGCAGCCAGTGTCATCTTCAGGTCCTGCCAATAGTTCAGGGACTGCTAATACCACTGGACCATCCCCCAGTTCACCTTCAACGCCTTCTACTCACACGGCAGGAGATGCGAACTCTGTGCCAACTTTGGCCCATAATAGCGGTTCCTCGAAGTCTCTGCTTATGTTTGCTTCTGATGGTCTAGGCTCAGTGCCAAATAAATTGAATGATATGGACCGATTTGTGGATGATGGATCTTTAGAGGATAATGTTGAATCATTCTTATCACATGATGATGCTGACCCTAGGGATAGAGTTGCTCGGTGTTCAGATGTCAGCAAAGGCTTCACTTTTTCGGAAGTTGGGGTTATTACTGCAAGTTCAAATAAAGTCGAGTGCTGTCACTTCTCAGCAGATGGGAAAACACTTGCCACTGGTGGGCATGATCGAAAGGCTGTATTGTGGTCTACAGAGTCCTTCACTGTAAAGTCTACGCTTGAAGAGCATTCTCAGTGGATAACGGATGTTCGATTCAGTCCTAGTATGTCAAGGCTAGCTACATCTTCTGCTGACAAAACTGTCAGGGTCTGGGATGTTGATAATCCTGGCTATTCACTTCGTACTTTTATGGGACATTCTACAACTGTTATGTCACTTGACTTCCACCCTACTAAAGAGGATCTTCTGTGCTCTTGTGATAACAACAGTGAGATAAGATACTGGAGTATCAAGAATGGTAGTTGTATTGGAGTTTCCAAGGGTGGTGCAACTCAGATGAGGTTTCAACCTCGTCTTGGAAGGATGCTTGCTGCTGCAGGTGATACTCTTGTGTCCATAATTGATGTAGAGACCGACTGTTGCACGCTTAAATTACAGGGTCATAAGAAACTTGTCAATTCTGTGTGCTGGGATTCTTCTGGTGAGTATCTAGCCTCTGTGAGTGATGACTCGGTTAGAGTATGGGCAGTTGGCTCCAGTAGCAGCAAAGGCGAATGCGTTTACGACTTAAGCTGTTCCGGCAACAAATATCAGACATGCGTCTTCCATCCTACTTATCCATCATTGTTGGTAATCGGCTGTTATGAGACATTGGAACTTTGGAACATGAGCGAGAACAAGACAATGACCTTGCATGCACATGACAACCTAGTATCTTGTTTGGCAGCGTCAAGTTCTACTGGCATGGTAGCTTCAGCTAGCCATGATAAGTTCGTGAAGCTATGGAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0001101 GO:0001558 GO:0001666 GO:0003002 GO:0003006 GO:0005975 GO:0005976 GO:0006355 GO:0006950 GO:0006974 GO:0006979 GO:0007275 GO:0007389 GO:0008150 GO:0008152 GO:0009414 GO:0009415 GO:0009605 GO:0009607 GO:0009611 GO:0009617 GO:0009620 GO:0009624 GO:0009628 GO:0009636 GO:0009719 GO:0009725 GO:0009733 GO:0009791 GO:0009798 GO:0009845 GO:0009888 GO:0009889 GO:0009890 GO:0009892 GO:0009909 GO:0009943 GO:0009944 GO:0009955 GO:0009987 GO:0010033 GO:0010035 GO:0010038 GO:0010073 GO:0010154 GO:0010191 GO:0010214 GO:0010243 GO:0010272 GO:0010393 GO:0010468 GO:0010556 GO:0010558 GO:0010605 GO:0010629 GO:0014070 GO:0019219 GO:0019222 GO:0022414 GO:0022603 GO:0022604 GO:0030307 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0032501 GO:0032502 GO:0032504 GO:0033554 GO:0036293 GO:0040008 GO:0042221 GO:0042493 GO:0043170 GO:0043207 GO:0044237 GO:0044238 GO:0045892 GO:0045927 GO:0045934 GO:0045995 GO:0046677 GO:0046898 GO:0048316 GO:0048359 GO:0048507 GO:0048509 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048580 GO:0048608 GO:0048609 GO:0048638 GO:0048639 GO:0048731 GO:0048831 GO:0048856 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051094 GO:0051128 GO:0051130 GO:0051171 GO:0051172 GO:0051239 GO:0051252 GO:0051253 GO:0051510 GO:0051512 GO:0051704 GO:0051707 GO:0051716 GO:0060255 GO:0060992 GO:0061458 GO:0065001 GO:0065007 GO:0070482 GO:0071216 GO:0071217 GO:0071496 GO:0071704 GO:0080001 GO:0080090 GO:0090351 GO:0097305 GO:1901654 GO:1901698 GO:1901700 GO:1902074 GO:1902183 GO:1902679 GO:1903506 GO:1903507 GO:2000024 GO:2000026 GO:2000112 GO:2000113 GO:2000241 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LisH WD40

Protein Analysis

729

Amino Acids

79.46

Weight (kDa)

6.09

Isoelectric Point (pI)

49.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LisH PF08513 10 - 36 2e-07 LisH
WD40_Gbeta PF25391 435 - 571 1.4e-10 G protein beta WD-40 repeat protein
Beta-prop_THOC3 PF25174 437 - 500 1.1e-08 THOC3 beta-propeller domain
WD40_MABP1-WDR62_2nd PF24782 438 - 641 4.6e-15 MABP1/WDR62 second WD40 domain
Beta-prop_TEP1_2nd PF25047 438 - 562 2.7e-13 TEP-1 second beta-propeller
EIF3I PF24805 439 - 521 1.3e-06 EIF3I
WD40_WDHD1_1st PF24817 439 - 521 3.3e-12 WDHD1 first WD40 domain
Beta-prop_WDR3_2nd PF25172 441 - 642 5.6e-15 WDR3 second beta-propeller domain
WD40_CDC20-Fz PF24807 443 - 570 5.7e-16 CDC20/Fizzy WD40 domain
WD40_Prp19 PF24814 446 - 644 5.4e-32 Prp19 WD40 domain
Beta-prop_EML_2 PF23414 446 - 567 4.7e-19 Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_WDR5 PF25175 446 - 570 2.4e-26 WDR5 beta-propeller domain
Beta-prop_WDR3_1st PF25173 446 - 563 1.2e-22 WDR3 first beta-propeller domain
Beta-prop_WDR36-Utp21_2nd PF25168 448 - 556 6e-08 WDR36/Utp21 second beta-propeller domain
Beta-prop_EML PF23409 449 - 568 3.6e-07 Echinoderm microtubule-associated protein first beta-propeller
Beta-prop_CAF1B_HIR1 PF24105 451 - 521 1.7e-07 CAF1B/HIR1 beta-propeller domain
WD40 PF00400 483 - 518 2.7e-09 WD domain, G-beta repeat
WDR55 PF24796 483 - 729 6.3e-12 WDR55
Beta-prop_THOC3 PF25174 489 - 729 6.2e-38 THOC3 beta-propeller domain
Beta-prop_WDR90_POC16_2nd PF23393 500 - 604 5.3e-07 WDR90/POC16, second beta-propeller
Beta-prop_WDR36-Utp21_2nd PF25168 549 - 646 6.7e-07 WDR36/Utp21 second beta-propeller domain
Beta-prop_WDR5 PF25175 574 - 728 4.4e-24 WDR5 beta-propeller domain
Beta-prop_WDR3_1st PF25173 575 - 673 4.5e-10 WDR3 first beta-propeller domain
WD40_WDHD1_1st PF24817 577 - 729 1.3e-13 WDHD1 first WD40 domain
WD40_Gbeta PF25391 579 - 728 1.6e-08 G protein beta WD-40 repeat protein
WD40 PF00400 607 - 641 1.3e-07 WD domain, G-beta repeat
WD40 PF00400 694 - 728 7.7e-06 WD domain, G-beta repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000670)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G32551 AT4G32551
fragaria_vesca FvH4_1g04170 FvH4_1g04170 FvH4_1g04170 FvH4_1g04170 FvH4_1g04170 FvH4_1g04170 FvH4_1g04170 FvH4_1g04170 FvH4_2g29940 FvH4_2g29940 FvH4_2g29940 FvH4_2g29940 FvH4_2g29940 FvH4_2g29940 FvH4_2g29940 FvH4_2g29940
malus_domestica MD02G1040200.v1.1 MD08G1139100.v1.1 MD15G1117100.v1.1 MD15G1145100.v1.1
prunus_persica Prupe.1G468400_v2.0.a1 Prupe.1G468400_v2.0.a1 Prupe.1G468400_v2.0.a1 Prupe.1G468400_v2.0.a1 Prupe.1G468400_v2.0.a1 Prupe.1G468400_v2.0.a1 Prupe.1G468400_v2.0.a1 Prupe.1G468400_v2.0.a1 Prupe.1G468400_v2.0.a1 Prupe.1G468400_v2.0.a1 Prupe.1G468400_v2.0.a1 Prupe.1G468400_v2.0.a1 Prupe.1G468400_v2.0.a1 Prupe.1G468400_v2.0.a1 Prupe.1G468400_v2.0.a1 Prupe.1G468400_v2.0.a1 Prupe.1G468400_v2.0.a1 Prupe.7G235500_v2.0.a1 Prupe.7G235500_v2.0.a1 Prupe.7G235500_v2.0.a1 Prupe.7G235500_v2.0.a1
pyrus_communis pycom02g03350 pycom08g11750 pycom15g10550 pycom15g16250
rosa_chinensis RchiOBHm_Chr2g0089671 RchiOBHm_Chr3g0465211 RchiOBHm_Chr6g0312941
rosa_laevigata RLG00000010283 RLG00000016050
rosa_multiflora Rmu_sc0000665.1_g000023 Rmu_sc0008049.1_g000043 Rmu_sc0008049.1_g000044
rosa_roxburghii Rroxscaffold_2G00151660 Rroxscaffold_7G00156310
rosa_rugosa Rorug02G0002500 Rorug02G0002500 Rorug06G0400700 Rorug06G0400700 Rorug06G0400700 Rorug06G0400700 Rorug06G0400800.1 Rorug06G0400900.1
rosa_samantha Rh2AG047900 Rh2BG046500 Rh2CG048500 Rh2DG047900 Rh6AG517700 Rh6BG529400 Rh6CG533900 Rh6DG520500
rosa_wichuraiana Rw2G004260 Rw5G017770 Rw6G045010

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 398, 1766
AasI GACNNNNNNGTC 1 cut(s) 1538
Acc36I ACCTGC 3 cut(s) 398, 502, 1766
AccI GTMKAC 2 cut(s) 1427, 1451
AciI CCGC 1 cut(s) 1122
AclI AACGTT 2 cut(s) 684, 699
AclWI GGATC 4 cut(s) 116, 712, 1222, 1647
AcoI YGGCCR 1 cut(s) 412
AcsI RAATTY 4 cut(s) 70, 525, 541, 610
AcuI CTGAAG 7 cut(s) 83, 123, 237, 515, 581, 984, 2136
AdeI CACNNNGTG 1 cut(s) 146
AfaI GTAC 4 cut(s) 278, 442, 693, 1582
AfiI CCNNNNNNNGG 5 cut(s) 306, 701, 1204, 1398, 1754
AflII CTTAAG 1 cut(s) 1966
AjnI CCWGG 5 cut(s) 445, 499, 520, 736, 1564
AloI GAACNNNNNNTCC 2 cut(s) 696, 728
Alw21I GWGCWC 1 cut(s) 1652
Alw26I GTCTC 4 cut(s) 423, 1140, 1802, 2039
AlwI GGATC 4 cut(s) 116, 712, 1222, 1647
AlwNI CAGNNNCTG 5 cut(s) 125, 781, 1007, 1025, 1548
AoxI GGCC 3 cut(s) 9, 412, 1110
ApoI RAATTY 4 cut(s) 70, 525, 541, 610
Asp700I GAANNNNTTC 1 cut(s) 112
AspA2I CCTAGG 1 cut(s) 1265
AspS9I GGNCC 4 cut(s) 1004, 1040, 1111, 1195
AsuC2I CCSGG 2 cut(s) 144, 423
AsuHPI GGTGA 5 cut(s) 359, 403, 1047, 1790, 1892
AsuII TTCGAA 2 cut(s) 159, 857
AsuNHI GCTAGC 2 cut(s) 1513, 2156
AvaII GGWCC 3 cut(s) 1004, 1040, 1195
AvrII CCTAGG 1 cut(s) 1265
BaeI ACNNNNGTAYC 6 cut(s) 1669, 1669, 1702, 1702, 1773, 1806
BalI TGGCCA 1 cut(s) 414
BbsI GAAGAC 2 cut(s) 166, 1993
Bbv12I GWGCWC 1 cut(s) 1652
BccI CCATC 7 cut(s) 631, 1051, 1151, 1205, 1370, 2013, 2026
BceAI ACGGC 1 cut(s) 1095
BcgI CGANNNNNNTGC 4 cut(s) 1081, 1115, 1120, 1154
BciT130I CCWGG 5 cut(s) 447, 501, 522, 738, 1566
BclI TGATCA 1 cut(s) 946
BcnI CCSGG 2 cut(s) 144, 423
BcoDI GTCTC 4 cut(s) 423, 1140, 1802, 2039
BfaI CTAG 9 cut(s) 195, 645, 1163, 1266, 1500, 1514, 1889, 2108, 2157
BfmI CTRYAG 4 cut(s) 117, 126, 1428, 1773
BfrI CTTAAG 1 cut(s) 1966
BfuAI ACCTGC 3 cut(s) 398, 502, 1766
BlnI CCTAGG 1 cut(s) 1265
Bme1390I CCNGG 7 cut(s) 144, 423, 447, 501, 522, 738, 1566
Bme18I GGWCC 3 cut(s) 1004, 1040, 1195
BmgT120I GGNCC 4 cut(s) 1004, 1040, 1111, 1195
BmiI GGNNCC 3 cut(s) 954, 1126, 1933
BmrFI CCNGG 7 cut(s) 144, 423, 447, 501, 522, 738, 1566
BmrI ACTGGG 1 cut(s) 1044
BmtI GCTAGC 2 cut(s) 1517, 2160
BmuI ACTGGG 1 cut(s) 1044
BoxI GACNNNNGTC 1 cut(s) 1997
BpiI GAAGAC 2 cut(s) 166, 1993
BpmI CTGGAG 4 cut(s) 468, 563, 1702, 1918
Bpu10I CCTNAGC 1 cut(s) 6
Bpu14I TTCGAA 2 cut(s) 159, 857
BpuMI CCSGG 2 cut(s) 144, 423
Bsa29I ATCGAT 1 cut(s) 656
BsaBI GATNNNNATC 1 cut(s) 375
BsaI GGTCTC 1 cut(s) 1802
BsaJI CCNNGG 2 cut(s) 1265, 1716
Bsc4I CCNNNNNNNGG 5 cut(s) 306, 701, 1204, 1398, 1754
Bse1I ACTGG 9 cut(s) 415, 895, 989, 1042, 1050, 1399, 1685, 1935, 2143
Bse3DI GCAATG 2 cut(s) 189, 949
Bse8I GATNNNNATC 1 cut(s) 375
BseBI CCWGG 5 cut(s) 447, 501, 522, 738, 1566
BseCI ATCGAT 1 cut(s) 656
BseDI CCNNGG 2 cut(s) 1265, 1716
BseJI GATNNNNATC 1 cut(s) 375
BseLI CCNNNNNNNGG 5 cut(s) 306, 701, 1204, 1398, 1754
BseMI GCAATG 2 cut(s) 189, 949
BseMII CTCAG 7 cut(s) 20, 575, 863, 1182, 1383, 1484, 1745
BseNI ACTGG 9 cut(s) 415, 895, 989, 1042, 1050, 1399, 1685, 1935, 2143
BseRI GAGGAG 1 cut(s) 653
BseYI CCCAGC 1 cut(s) 1866
BsgI GTGCAG 1 cut(s) 762
BshFI GGCC 3 cut(s) 11, 414, 1112
BshVI ATCGAT 1 cut(s) 656
BsiHKAI GWGCWC 1 cut(s) 1652
BsiSI CCGG 3 cut(s) 144, 423, 1977
BslFI GGGAC 3 cut(s) 194, 1036, 1605
BslI CCNNNNNNNGG 5 cut(s) 306, 701, 1204, 1398, 1754
BsmAI GTCTC 4 cut(s) 423, 1140, 1802, 2039
BsmFI GGGAC 3 cut(s) 194, 1036, 1605
BsmI GAATGC 2 cut(s) 1465, 1958
BsnI GGCC 3 cut(s) 11, 414, 1112
Bso31I GGTCTC 1 cut(s) 1802
Bsp119I TTCGAA 2 cut(s) 159, 857
Bsp1286I GDGCHC 1 cut(s) 1652
Bsp143I GATC 7 cut(s) 121, 704, 885, 946, 1214, 1405, 1639
BspACI CCGC 1 cut(s) 1122
BspANI GGCC 3 cut(s) 11, 414, 1112
BspCNI CTCAG 7 cut(s) 19, 574, 862, 1181, 1382, 1483, 1744
BspDI ATCGAT 1 cut(s) 656
BspLI GGNNCC 3 cut(s) 954, 1126, 1933
BspMAI CTGCAG 1 cut(s) 1777
BspMI ACCTGC 3 cut(s) 398, 502, 1766
BspOI GCTAGC 2 cut(s) 1517, 2160
BspPI GGATC 4 cut(s) 116, 712, 1222, 1647
BspQI GCTCTTC 1 cut(s) 1455
BspT104I TTCGAA 2 cut(s) 159, 857
BspTI CTTAAG 1 cut(s) 1966
BspTNI GGTCTC 1 cut(s) 1802
BsrDI GCAATG 2 cut(s) 189, 949
BsrI ACTGG 9 cut(s) 415, 895, 989, 1042, 1050, 1399, 1685, 1935, 2143
BssECI CCNNGG 2 cut(s) 1265, 1716
BssMI GATC 7 cut(s) 121, 704, 885, 946, 1214, 1405, 1639
BssT1I CCWWGG 2 cut(s) 1265, 1716
Bst2UI CCWGG 5 cut(s) 447, 501, 522, 738, 1566
Bst4CI ACNGT 6 cut(s) 816, 1444, 1540, 1606, 1667, 1817
Bst6I CTCTTC 2 cut(s) 59, 1455
BstAFI CTTAAG 1 cut(s) 1966
BstAPI GCANNNNNTGC 1 cut(s) 1088
BstBI TTCGAA 2 cut(s) 159, 857
BstC8I GCNNGC 6 cut(s) 898, 1515, 1767, 1823, 2094, 2158
BstDEI CTNAG 7 cut(s) 6, 561, 849, 1168, 1369, 1470, 1731
BstKTI GATC 7 cut(s) 124, 707, 888, 949, 1217, 1408, 1642
BstMAI GTCTC 4 cut(s) 423, 1140, 1802, 2039
BstMBI GATC 7 cut(s) 121, 704, 885, 946, 1214, 1405, 1639
BstNI CCWGG 5 cut(s) 447, 501, 522, 738, 1566
BstNSI RCATGY 4 cut(s) 80, 925, 1998, 2096
BstPAI GACNNNNGTC 1 cut(s) 1997
BstSCI CCNGG 7 cut(s) 142, 421, 445, 499, 520, 736, 1564
BstSFI CTRYAG 4 cut(s) 117, 126, 1428, 1773
BstV2I GAAGAC 2 cut(s) 166, 1993
BstX2I RGATCY 3 cut(s) 121, 1214, 1639
BstXI CCANNNNNNTGG 1 cut(s) 637
BstYI RGATCY 3 cut(s) 121, 1214, 1639
Bsu15I ATCGAT 1 cut(s) 656
BsuRI GGCC 3 cut(s) 11, 414, 1112
BsuTUI ATCGAT 1 cut(s) 656
BtsIMutI CAGTG 8 cut(s) 888, 996, 1035, 1176, 1392, 1440, 1478, 1672
BveI ACCTGC 3 cut(s) 398, 502, 1766
Cac8I GCNNGC 6 cut(s) 898, 1515, 1767, 1823, 2094, 2158
CaiI CAGNNNCTG 5 cut(s) 125, 781, 1007, 1025, 1548
Cfr13I GGNCC 4 cut(s) 1004, 1040, 1111, 1195
ClaI ATCGAT 1 cut(s) 656
CseI GACGC 2 cut(s) 1987, 2115
Csp6I GTAC 4 cut(s) 277, 441, 692, 1581
CviQI GTAC 4 cut(s) 277, 441, 692, 1581
DdeI CTNAG 7 cut(s) 6, 561, 849, 1168, 1369, 1470, 1731
DpnI GATC 7 cut(s) 123, 706, 887, 948, 1216, 1407, 1641
DpnII GATC 7 cut(s) 121, 704, 885, 946, 1214, 1405, 1639
DraIII CACNNNGTG 1 cut(s) 146
DrdI GACNNNNNNGTC 1 cut(s) 1538
DseDI GACNNNNNNGTC 1 cut(s) 1538
EaeI YGGCCR 1 cut(s) 412
Eam1104I CTCTTC 2 cut(s) 59, 1455
EarI CTCTTC 2 cut(s) 59, 1455
Eco130I CCWWGG 2 cut(s) 1265, 1716
Eco31I GGTCTC 1 cut(s) 1802
Eco47I GGWCC 3 cut(s) 1004, 1040, 1195
Eco57I CTGAAG 7 cut(s) 83, 123, 237, 515, 581, 984, 2136
EcoO109I RGGNCCY 1 cut(s) 1004
EcoRI GAATTC 1 cut(s) 525
EcoRII CCWGG 5 cut(s) 445, 499, 520, 736, 1564
EcoT14I CCWWGG 2 cut(s) 1265, 1716
ErhI CCWWGG 2 cut(s) 1265, 1716
FalI AAGNNNNNCTT 2 cut(s) 1626, 1658
FaqI GGGAC 3 cut(s) 194, 1036, 1605
FbaI TGATCA 1 cut(s) 946
FblI GTMKAC 2 cut(s) 1427, 1451
FspBI CTAG 9 cut(s) 195, 645, 1163, 1266, 1500, 1514, 1889, 2108, 2157
GsaI CCCAGC 1 cut(s) 1870
GsuI CTGGAG 4 cut(s) 468, 563, 1702, 1918
HaeIII GGCC 3 cut(s) 11, 414, 1112
HapII CCGG 3 cut(s) 144, 423, 1977
HgaI GACGC 2 cut(s) 1987, 2115
HincII GTYRAC 1 cut(s) 842
HindII GTYRAC 1 cut(s) 842
HpaII CCGG 3 cut(s) 144, 423, 1977
HphI GGTGA 5 cut(s) 359, 403, 1047, 1790, 1892
Hpy166II GTNNAC 6 cut(s) 443, 842, 1055, 1428, 1452, 1960
Hpy188I TCNGA 9 cut(s) 63, 217, 486, 852, 1156, 1291, 1318, 1734, 1992
Hpy188III TCNNGA 2 cut(s) 1690, 2173
Hpy8I GTNNAC 6 cut(s) 443, 842, 1055, 1428, 1452, 1960
HpyAV CCTTC 7 cut(s) 68, 98, 642, 1068, 1077, 1447, 1752
HpyCH4III ACNGT 6 cut(s) 816, 1444, 1540, 1606, 1667, 1817
HpyCH4IV ACGT 2 cut(s) 684, 699
HpyF3I CTNAG 7 cut(s) 6, 561, 849, 1168, 1369, 1470, 1731
HpySE526I ACGT 2 cut(s) 684, 699
Ksp22I TGATCA 1 cut(s) 946
Kzo9I GATC 7 cut(s) 121, 704, 885, 946, 1214, 1405, 1639
LguI GCTCTTC 1 cut(s) 1455
LmnI GCTCC 4 cut(s) 582, 666, 958, 1937
MaeI CTAG 9 cut(s) 195, 645, 1163, 1266, 1500, 1514, 1889, 2108, 2157
MaeII ACGT 2 cut(s) 684, 699
MaeIII GTNAC 4 cut(s) 347, 765, 1361, 1611
MalI GATC 7 cut(s) 123, 706, 887, 948, 1216, 1407, 1641
MboI GATC 7 cut(s) 121, 704, 885, 946, 1214, 1405, 1639
MfeI CAATTG 1 cut(s) 13
MflI RGATCY 3 cut(s) 121, 1214, 1639
MhlI GDGCHC 1 cut(s) 1652
MlsI TGGCCA 1 cut(s) 414
MluCI AATT 9 cut(s) 13, 70, 525, 541, 610, 1181, 1797, 1829, 1855
MluNI TGGCCA 1 cut(s) 414
MlyI GAGTC 3 cut(s) 496, 1442, 1901
MmeI TCCRAC 3 cut(s) 15, 682, 951
Mox20I TGGCCA 1 cut(s) 414
MroXI GAANNNNTTC 1 cut(s) 112
MscI TGGCCA 1 cut(s) 414
MseI TTAA 4 cut(s) 290, 570, 1827, 1967
MslI CAYNNNNRTG 2 cut(s) 1254, 2097
Msp20I TGGCCA 1 cut(s) 414
MspA1I CMGCKG 3 cut(s) 224, 781, 911
MspCI CTTAAG 1 cut(s) 1966
MspI CCGG 3 cut(s) 144, 423, 1977
MspR9I CCNGG 7 cut(s) 144, 423, 447, 501, 522, 738, 1566
MunI CAATTG 1 cut(s) 13
Mva1269I GAATGC 2 cut(s) 1465, 1958
MvaI CCWGG 5 cut(s) 447, 501, 522, 738, 1566
NciI CCSGG 2 cut(s) 144, 423
NdeII GATC 7 cut(s) 121, 704, 885, 946, 1214, 1405, 1639
NheI GCTAGC 2 cut(s) 1513, 2156
NlaIV GGNNCC 3 cut(s) 954, 1126, 1933
NmuCI GTSAC 3 cut(s) 347, 1361, 1611
NspI RCATGY 4 cut(s) 80, 925, 1998, 2096
NspV TTCGAA 2 cut(s) 159, 857
PaeI GCATGC 1 cut(s) 2096
PaqCI CACCTGC 2 cut(s) 398, 1766
PciSI GCTCTTC 1 cut(s) 1455
PctI GAATGC 2 cut(s) 1465, 1958
PdmI GAANNNNTTC 1 cut(s) 112
PfeI GAWTC 8 cut(s) 358, 371, 463, 859, 961, 1235, 1491, 1871
PfoI TCCNGGA 1 cut(s) 499
PleI GAGTC 3 cut(s) 495, 1441, 1901
PpsI GAGTC 3 cut(s) 495, 1441, 1901
PpuMI RGGWCCY 1 cut(s) 1004
PshAI GACNNNNGTC 1 cut(s) 1997
Psp1406I AACGTT 2 cut(s) 684, 699
Psp5II RGGWCCY 1 cut(s) 1004
Psp6I CCWGG 5 cut(s) 445, 499, 520, 736, 1564
PspFI CCCAGC 1 cut(s) 1866
PspGI CCWGG 5 cut(s) 445, 499, 520, 736, 1564
PspN4I GGNNCC 3 cut(s) 954, 1126, 1933
PspPI GGNCC 4 cut(s) 1004, 1040, 1111, 1195
PspPPI RGGWCCY 1 cut(s) 1004
PstI CTGCAG 1 cut(s) 1777
PstNI CAGNNNCTG 5 cut(s) 125, 781, 1007, 1025, 1548
PsuI RGATCY 3 cut(s) 121, 1214, 1639
PvuII CAGCTG 3 cut(s) 224, 781, 911
RsaI GTAC 4 cut(s) 278, 442, 693, 1582
RsaNI GTAC 4 cut(s) 277, 441, 692, 1581
RseI CAYNNNNRTG 2 cut(s) 1254, 2097
SapI GCTCTTC 1 cut(s) 1455
SaqAI TTAA 4 cut(s) 290, 570, 1827, 1967
Sau3AI GATC 7 cut(s) 121, 704, 885, 946, 1214, 1405, 1639
Sau96I GGNCC 4 cut(s) 1004, 1040, 1111, 1195
SchI GAGTC 3 cut(s) 496, 1442, 1901
ScrFI CCNGG 7 cut(s) 144, 423, 447, 501, 522, 738, 1566
SduI GDGCHC 1 cut(s) 1652
SfcI CTRYAG 4 cut(s) 117, 126, 1428, 1773
SfuI TTCGAA 2 cut(s) 159, 857
SinI GGWCC 3 cut(s) 1004, 1040, 1195
SmiMI CAYNNNNRTG 2 cut(s) 1254, 2097
SmlI CTYRAG 1 cut(s) 1966
SmoI CTYRAG 1 cut(s) 1966
SphI GCATGC 1 cut(s) 2096
Sse9I AATT 9 cut(s) 13, 70, 525, 541, 610, 1181, 1797, 1829, 1855
SsiI CCGC 1 cut(s) 1122
SspMI CTAG 9 cut(s) 195, 645, 1163, 1266, 1500, 1514, 1889, 2108, 2157
StyD4I CCNGG 7 cut(s) 142, 421, 445, 499, 520, 736, 1564
StyI CCWWGG 2 cut(s) 1265, 1716
TaaI ACNGT 6 cut(s) 816, 1444, 1540, 1606, 1667, 1817
TaiI ACGT 2 cut(s) 687, 702
TaqI TCGA 8 cut(s) 159, 517, 656, 857, 1130, 1353, 1408, 1489
TaqII GACCGA 2 cut(s) 1212, 1826
TasI AATT 9 cut(s) 13, 70, 525, 541, 610, 1181, 1797, 1829, 1855
TfiI GAWTC 8 cut(s) 358, 371, 463, 859, 961, 1235, 1491, 1871
Tru1I TTAA 4 cut(s) 290, 570, 1827, 1967
Tru9I TTAA 4 cut(s) 290, 570, 1827, 1967
TscAI CASTG 8 cut(s) 895, 996, 1042, 1176, 1399, 1447, 1478, 1672
TseFI GTSAC 3 cut(s) 347, 1361, 1611
Tsp45I GTSAC 3 cut(s) 347, 1361, 1611
TspDTI ATGAA 5 cut(s) 178, 219, 347, 371, 776
TspGWI ACGGA 1 cut(s) 1496
TspRI CASTG 8 cut(s) 895, 996, 1042, 1176, 1399, 1447, 1478, 1672
Vha464I CTTAAG 1 cut(s) 1966
VpaK11BI GGWCC 3 cut(s) 1004, 1040, 1195
XapI RAATTY 4 cut(s) 70, 525, 541, 610
XceI RCATGY 4 cut(s) 80, 925, 1998, 2096
XmaJI CCTAGG 1 cut(s) 1265
XmiI GTMKAC 2 cut(s) 1427, 1451
XmnI GAANNNNTTC 1 cut(s) 112
XspI CTAG 9 cut(s) 195, 645, 1163, 1266, 1500, 1514, 1889, 2108, 2157
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.