MD15G1145100.v1.1

Transcriptional corepressor LEUNIG-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0000670
Physical Location & Seq
Forward (+)
10745957 .. 10752429
6473 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1145100.v1.1.491

Sequence Viewer

Length: 2577 bp
ATGTCTCAGAGCCAGAACAGTTGGGAAGCTGATAAAATGTTGGATGTGTATATATATGATTACCTTATGAAGAGAAAATTACATGCTTCTGCAAAGGCATTTCAAGCTGAAGGAAAAGTTGCTACGGATCCTGTAGCTATTGATGCACCTGGCGGCTTTCTTTTCGAATGGTGGTCTGTCTTTTGGGACATATTCATTGCTAGGACGAATGAGAAGCACTCAGAAGCAGCTGCATCTTACATTGAGACTCAAGCGATAAAGGCTAGGGAGCAGCATCAGAAGCCTCAGCAGCAAGAGCAAATACAAATGCAGCAGTTTAGATTACAGAGACATGCTCAGCAGCAGCAGCAGCAGCGACAGCAGCAGCAGCAGCAGCAGCAACAGCAGCAACAGCAACAGCAACAGCAACAGCAGCTTCGAGATGGGACCCAGCTTCATAATGGCACTTCTAATGATTCTCTTTTGAGGCAGAACCCTGCAACTGCAAATTCTATGGCAACAAAAATGTACGAGGAGAGATTAAAGCTTCCAACTCAGAGGGATGCTATGGACGATGCAGCTATCAAGCAAAGGTTAGGAGACAATATGAGTCAGCTTTTGGATCCCAATCATGCCTCGATGATGAAAGCAACTACAGCAGGTGGCCAGCCTCCTGGTCAAATGCTGCATGGTACACCTGGTGGTGTGTTGGGGAATCTTCAACAACCTCACAGTCGGAGTCAGCAACTTCCTGGTTCTTCACAGGACATAAAGAGTGAGATGATGAATCCCAGAGCTGTTGCTCCAGAAGGATCATTGATTGGTGCTCACGGATCAAATCAAGGCAGTAACAATTTGACTCTGAAAGGATGGCCTTTAACGGGATTTGATCGGCTTCGATCTGGGATTCTTCAGCAGCAAAATCCTTTGATGCAGTCCCCGCAACCGTATAATCAACTTCTGCAACAGCAGCAACTTATGCTGGCACAACAAAATTTACCTTCTCCATCTTCCAATGACTTGGACAGTAGAAGGATGAGAATGCTTCTCAACAATCGAAATATGGTTCTTGGGAAGGATGGTCAATTAAGTTCTGTTGATGTACCTAATGCTGGATCACCAGTGCAAGTTGGTTGCCCGGTCTTACCTCGTGGAGATGCTGACATGCTTATGAAGTTACAGCAACAGCAGATGCAAAGCAACAATCAACAGCAGCAGCAGTATTTGCAGCATCTGCTTTCAAGTCAGCACTCTCATAATTCAAGCCAACACCTCCAGCAGCATGAAAAAATTATGGGTTCTGGTGGCATGGCACCAGATGGTAGCATGCCTAACACCTTTCAAGGGAATGATCAGGCTTCAAAGAATCAACTTGGGCGAAAGAGAAAGCAGCCAGTGTCATCTTCGGGTCCTGCCAATAGTTCAGGGACAGTTAACACCACGGGACCATCTCCCAGTTCACCTTCAACACCTTCTACTCACACAGCGGATGGCGTGTCTATGCCGACTTTACCCCCTAATGGTGGTTCGTCGAAGTCTGTACTTATGTTTGGCTCTGATGGCTTGGGCTCAATTGCATCAGCACCAAATAAATTGACTGATGTAGACCGTTTTGTGGATGATGGATCTTTGGAGGATAATGTTGAATCGTTCTTATCGCATGATGATGCTGACCCTAGAGGTAGAGTTGCTCGGTGTTCAGATGTTGGCAAAGGCTTCAGTTTTACGGAAGTCCAGCTTATTCCTGCAAGTAAAAATAAAGTTGAATGTTGCCACTTCTCTTCAGATGGAAAATCACTTGCCACTGGTGGGCATGACCGAAAGGCTGTATTGTGGTGCACTGAAACCTATAGTGTCAAGTCCACACTTGAAGAGCATTCTCAATGGATAACTGACGTTCGCTTTAGTCCTAGCATGTCAAGGCTTGCTACATCTTCTGCAGATAAAACCGTCCGGGTTTGGGATGCTGATAATCCTGGATATTCACTTCGTAATTTTGTGGGACATTCTGCCACTGTTGTGTCAGTGGACTTCCATCCCAGTAAAGAGGACCTTCTCTGCTCTTGTGATAACAACAGTGAGATGCGGTACTGGAGTATCAAGAATGGTAGTTGTGCTGGAGTTTTCAAGGGTGGTGCAACTCAGATGAGGTTTCAACCTCGTTTTGGAAGAAACCTTGCTGCTGCAGCAGATAATTTTGTATCAATCTTGGATGTTGAGACACAAGTTTGCAGGCTTAAATTACAGGGACATAAAAGCCATGTGCATTCTGTGTGCTGGGATCCTTCTGGCGAGTATCTAGCATCAGTGAGTGACGATTTGGTTCGAGTGTGGACAGTTGGTTCCAGCTGCAAAGGGGAATTGATTCACGAGTTGAGCTGTTCCGGCAACAAATTCAAAACGTGTGTGTTCCATCCGACTTATCCTGCATTGTTGGTGATTGGCTGTTATGAGACTTTGGAGCTTTGGAACATGGCCGAGAACAAGACAATGACTCTGCACGCTCATGACAAGCTAGTGTCTTCTCTGGCGGTATCAAATGCTACGGGGCTAGTTGCTTCGGCCAGCCACGACAAGTGCGTCAAGCTCTGGAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0001101 GO:0001558 GO:0001666 GO:0003002 GO:0003006 GO:0005975 GO:0005976 GO:0006355 GO:0006950 GO:0006974 GO:0006979 GO:0007275 GO:0007389 GO:0008150 GO:0008152 GO:0009414 GO:0009415 GO:0009605 GO:0009607 GO:0009611 GO:0009617 GO:0009620 GO:0009624 GO:0009628 GO:0009636 GO:0009719 GO:0009725 GO:0009733 GO:0009791 GO:0009798 GO:0009845 GO:0009888 GO:0009889 GO:0009890 GO:0009892 GO:0009909 GO:0009943 GO:0009944 GO:0009955 GO:0009987 GO:0010033 GO:0010035 GO:0010038 GO:0010073 GO:0010154 GO:0010191 GO:0010214 GO:0010243 GO:0010272 GO:0010393 GO:0010468 GO:0010556 GO:0010558 GO:0010605 GO:0010629 GO:0014070 GO:0019219 GO:0019222 GO:0022414 GO:0022603 GO:0022604 GO:0030307 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0032501 GO:0032502 GO:0032504 GO:0033554 GO:0036293 GO:0040008 GO:0042221 GO:0042493 GO:0043170 GO:0043207 GO:0044237 GO:0044238 GO:0045892 GO:0045927 GO:0045934 GO:0045995 GO:0046677 GO:0046898 GO:0048316 GO:0048359 GO:0048507 GO:0048509 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048580 GO:0048608 GO:0048609 GO:0048638 GO:0048639 GO:0048731 GO:0048831 GO:0048856 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051094 GO:0051128 GO:0051130 GO:0051171 GO:0051172 GO:0051239 GO:0051252 GO:0051253 GO:0051510 GO:0051512 GO:0051704 GO:0051707 GO:0051716 GO:0060255 GO:0060992 GO:0061458 GO:0065001 GO:0065007 GO:0070482 GO:0071216 GO:0071217 GO:0071496 GO:0071704 GO:0080001 GO:0080090 GO:0090351 GO:0097305 GO:1901654 GO:1901698 GO:1901700 GO:1902074 GO:1902183 GO:1902679 GO:1903506 GO:1903507 GO:2000024 GO:2000026 GO:2000112 GO:2000113 GO:2000241 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LisH WD40

Protein Analysis

859

Amino Acids

94.48

Weight (kDa)

7.37

Isoelectric Point (pI)

50.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LisH PF08513 12 - 38 3.4e-07 LisH
WD40_MABP1-WDR62_2nd PF24782 568 - 771 3.6e-14 MABP1/WDR62 second WD40 domain
WD40_Gbeta PF25391 568 - 706 2e-09 G protein beta WD-40 repeat protein
Beta-prop_THOC3 PF25174 569 - 630 1.4e-07 THOC3 beta-propeller domain
WDR55 PF24796 570 - 694 6.1e-06 WDR55
EIF3I PF24805 570 - 651 2e-06 EIF3I
WD40_CDC20-Fz PF24807 573 - 699 7.8e-14 CDC20/Fizzy WD40 domain
Beta-prop_WDR3_1st PF25173 576 - 693 4.9e-21 WDR3 first beta-propeller domain
Beta-prop_TEP1_2nd PF25047 576 - 693 5.3e-13 TEP-1 second beta-propeller
Beta-prop_WDR5 PF25175 577 - 703 1.3e-24 WDR5 beta-propeller domain
WD40_Prp19 PF24814 577 - 772 2.4e-30 Prp19 WD40 domain
Beta-prop_EML_2 PF23414 577 - 697 4.5e-17 Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_THOC3 PF25174 578 - 651 2e-15 THOC3 beta-propeller domain
Beta-prop_WDR36-Utp21_2nd PF25168 578 - 680 5.8e-07 WDR36/Utp21 second beta-propeller domain
WD40_WDHD1_1st PF24817 578 - 651 1.3e-09 WDHD1 first WD40 domain
Beta-prop_CAF1B_HIR1 PF24105 581 - 651 9e-07 CAF1B/HIR1 beta-propeller domain
Beta-prop_WDR3_2nd PF25172 581 - 691 6.2e-10 WDR3 second beta-propeller domain
WD40 PF00400 613 - 648 3.8e-09 WD domain, G-beta repeat
WDR55 PF24796 617 - 858 4.9e-08 WDR55
Beta-prop_THOC3 PF25174 619 - 858 4.2e-34 THOC3 beta-propeller domain
WD40_WDHD1_1st PF24817 623 - 703 4e-09 WDHD1 first WD40 domain
Beta-prop_WDR90_POC16_2nd PF23393 630 - 696 2.5e-06 WDR90/POC16, second beta-propeller
EIF3I PF24805 660 - 857 3.9e-07 EIF3I
Beta-prop_WDR36-Utp21_2nd PF25168 678 - 777 9.2e-07 WDR36/Utp21 second beta-propeller domain
Beta-prop_WDR5 PF25175 705 - 857 1e-23 WDR5 beta-propeller domain
WD40_WDHD1_1st PF24817 706 - 858 9.5e-13 WDHD1 first WD40 domain
Beta-prop_WDR3_1st PF25173 707 - 858 2.1e-16 WDR3 first beta-propeller domain
WD40_Prp19 PF24814 709 - 857 1.9e-15 Prp19 WD40 domain
WD40_Gbeta PF25391 712 - 857 1.1e-08 G protein beta WD-40 repeat protein
Beta-prop_WDR36-Utp21_1st PF25171 719 - 853 8e-07 WDR36/Utp21 first beta-propeller
WD40_CDC20-Fz PF24807 724 - 857 4.4e-09 CDC20/Fizzy WD40 domain
WD40 PF00400 737 - 771 8.5e-08 WD domain, G-beta repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000670)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G32551 AT4G32551
fragaria_vesca FvH4_1g04170 FvH4_1g04170 FvH4_1g04170 FvH4_1g04170 FvH4_1g04170 FvH4_1g04170 FvH4_1g04170 FvH4_1g04170 FvH4_2g29940 FvH4_2g29940 FvH4_2g29940 FvH4_2g29940 FvH4_2g29940 FvH4_2g29940 FvH4_2g29940 FvH4_2g29940
malus_domestica MD02G1040200.v1.1 MD08G1139100.v1.1 MD15G1117100.v1.1 MD15G1145100.v1.1
prunus_persica Prupe.1G468400_v2.0.a1 Prupe.1G468400_v2.0.a1 Prupe.1G468400_v2.0.a1 Prupe.1G468400_v2.0.a1 Prupe.1G468400_v2.0.a1 Prupe.1G468400_v2.0.a1 Prupe.1G468400_v2.0.a1 Prupe.1G468400_v2.0.a1 Prupe.1G468400_v2.0.a1 Prupe.1G468400_v2.0.a1 Prupe.1G468400_v2.0.a1 Prupe.1G468400_v2.0.a1 Prupe.1G468400_v2.0.a1 Prupe.1G468400_v2.0.a1 Prupe.1G468400_v2.0.a1 Prupe.1G468400_v2.0.a1 Prupe.1G468400_v2.0.a1 Prupe.7G235500_v2.0.a1 Prupe.7G235500_v2.0.a1 Prupe.7G235500_v2.0.a1 Prupe.7G235500_v2.0.a1
pyrus_communis pycom02g03350 pycom08g11750 pycom15g10550 pycom15g16250
rosa_chinensis RchiOBHm_Chr2g0089671 RchiOBHm_Chr3g0465211 RchiOBHm_Chr6g0312941
rosa_laevigata RLG00000010283 RLG00000016050
rosa_multiflora Rmu_sc0000665.1_g000023 Rmu_sc0008049.1_g000043 Rmu_sc0008049.1_g000044
rosa_roxburghii Rroxscaffold_2G00151660 Rroxscaffold_7G00156310
rosa_rugosa Rorug02G0002500 Rorug02G0002500 Rorug06G0400700 Rorug06G0400700 Rorug06G0400700 Rorug06G0400700 Rorug06G0400800.1 Rorug06G0400900.1
rosa_samantha Rh2AG047900 Rh2BG046500 Rh2CG048500 Rh2DG047900 Rh6AG517700 Rh6BG529400 Rh6CG533900 Rh6DG520500
rosa_wichuraiana Rw2G004260 Rw5G017770 Rw6G045010

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 629
AasI GACNNNNNNGTC 1 cut(s) 2558
Acc36I ACCTGC 1 cut(s) 629
AccB1I GGYRCC 1 cut(s) 1291
AccI GTMKAC 1 cut(s) 1584
AciI CCGC 5 cut(s) 153, 920, 1466, 2065, 2510
AcoI YGGCCR 3 cut(s) 643, 2454, 2541
AcsI RAATTY 3 cut(s) 487, 973, 2372
AcuI CTGAAG 4 cut(s) 129, 875, 1681, 1746
AdeI CACNNNGTG 1 cut(s) 680
AfaI GTAC 5 cut(s) 509, 673, 1083, 1521, 2069
AfiI CCNNNNNNNGG 9 cut(s) 860, 1091, 1323, 1499, 1502, 1594, 1788, 1939, 2144
AflIII ACRYGT 1 cut(s) 2381
AjnI CCWGG 5 cut(s) 148, 652, 676, 730, 1954
AleI CACNNNNGTG 1 cut(s) 1997
AloI GAACNNNNNNTCC 2 cut(s) 2305, 2337
Alw21I GWGCWC 2 cut(s) 808, 1820
Alw26I GTCTC 6 cut(s) 9, 239, 322, 573, 2192, 2426
Alw44I GTGCAC 1 cut(s) 1816
AlwNI CAGNNNCTG 1 cut(s) 1213
AoxI GGCC 4 cut(s) 643, 851, 2454, 2541
ApaLI GTGCAC 1 cut(s) 1816
ApoI RAATTY 3 cut(s) 487, 973, 2372
Asp700I GAANNNNTTC 1 cut(s) 2343
AspS9I GGNCC 4 cut(s) 426, 1388, 1424, 2029
AsuC2I CCSGG 2 cut(s) 1118, 1934
AsuHPI GGTGA 3 cut(s) 1089, 1431, 2428
AsuII TTCGAA 1 cut(s) 165
AvaII GGWCC 4 cut(s) 426, 1388, 1424, 2029
BaeGI GKGCMC 1 cut(s) 1820
BaeI ACNNNNGTAYC 4 cut(s) 2059, 2059, 2092, 2092
BalI TGGCCA 1 cut(s) 645
BamHI GGATCC 3 cut(s) 127, 601, 2260
BanI GGYRCC 1 cut(s) 1291
BanII GRGCYC 1 cut(s) 1550
BauI CACGAG 2 cut(s) 1128, 2348
BbsI GAAGAC 1 cut(s) 2493
Bbv12I GWGCWC 2 cut(s) 808, 1820
BbvCI CCTCAGC 1 cut(s) 285
BciT130I CCWGG 5 cut(s) 150, 654, 678, 732, 1956
BclI TGATCA 1 cut(s) 1330
BcnI CCSGG 2 cut(s) 1118, 1934
BcoDI GTCTC 6 cut(s) 9, 239, 322, 573, 2192, 2426
BfaI CTAG 7 cut(s) 201, 264, 1656, 1890, 2279, 2495, 2531
BfmI CTRYAG 5 cut(s) 132, 633, 1828, 1917, 2163
BfuAI ACCTGC 1 cut(s) 629
BlpI GCTNAGC 1 cut(s) 336
Bme1390I CCNGG 7 cut(s) 150, 654, 678, 732, 1118, 1934, 1956
Bme18I GGWCC 4 cut(s) 426, 1388, 1424, 2029
BmgT120I GGNCC 4 cut(s) 426, 1388, 1424, 2029
BmiI GGNNCC 9 cut(s) 129, 427, 428, 603, 1293, 1389, 1425, 2262, 2323
BmrFI CCNGG 7 cut(s) 150, 654, 678, 732, 1118, 1934, 1956
BmrI ACTGGG 2 cut(s) 1428, 2013
BmuI ACTGGG 2 cut(s) 1428, 2013
BpiI GAAGAC 1 cut(s) 2493
BplI GAGNNNNNCTC 4 cut(s) 203, 235, 319, 351
BpmI CTGGAG 4 cut(s) 768, 1238, 2092, 2118
Bpu10I CCTNAGC 1 cut(s) 285
Bpu1102I GCTNAGC 1 cut(s) 336
Bpu14I TTCGAA 1 cut(s) 165
BpuEI CTTGAG 1 cut(s) 234
BpuMI CCSGG 2 cut(s) 1118, 1934
BsaBI GATNNNNATC 1 cut(s) 606
BsaJI CCNNGG 1 cut(s) 1419
Bsc4I CCNNNNNNNGG 9 cut(s) 860, 1091, 1323, 1499, 1502, 1594, 1788, 1939, 2144
Bse1I ACTGG 6 cut(s) 1100, 1373, 1434, 1789, 2019, 2075
Bse3DI GCAATG 1 cut(s) 195
Bse8I GATNNNNATC 1 cut(s) 606
BseBI CCWGG 5 cut(s) 150, 654, 678, 732, 1956
BseDI CCNNGG 1 cut(s) 1419
BseJI GATNNNNATC 1 cut(s) 606
BseLI CCNNNNNNNGG 9 cut(s) 860, 1091, 1323, 1499, 1502, 1594, 1788, 1939, 2144
BseMI GCAATG 1 cut(s) 195
BseMII CTCAG 6 cut(s) 20, 234, 299, 350, 548, 2135
BseNI ACTGG 6 cut(s) 1100, 1373, 1434, 1789, 2019, 2075
BseRI GAGGAG 1 cut(s) 527
BseSI GKGCMC 1 cut(s) 1820
BseYI CCCAGC 2 cut(s) 429, 2256
BsgI GTGCAG 1 cut(s) 2462
BshFI GGCC 4 cut(s) 645, 853, 2456, 2543
BshNI GGYRCC 1 cut(s) 1291
BsiHKAI GWGCWC 2 cut(s) 808, 1820
BsiSI CCGG 3 cut(s) 1118, 1933, 2364
BslFI GGGAC 7 cut(s) 200, 439, 901, 1420, 1437, 1995, 2241
BslI CCNNNNNNNGG 9 cut(s) 860, 1091, 1323, 1499, 1502, 1594, 1788, 1939, 2144
BsmAI GTCTC 6 cut(s) 9, 239, 322, 573, 2192, 2426
BsmFI GGGAC 7 cut(s) 200, 439, 901, 1420, 1437, 1995, 2241
BsmI GAATGC 3 cut(s) 1026, 1855, 2245
BsnI GGCC 4 cut(s) 645, 853, 2456, 2543
Bsp119I TTCGAA 1 cut(s) 165
Bsp1286I GDGCHC 3 cut(s) 808, 1550, 1820
Bsp1720I GCTNAGC 1 cut(s) 336
BspACI CCGC 5 cut(s) 153, 920, 1466, 2065, 2510
BspANI GGCC 4 cut(s) 645, 853, 2456, 2543
BspCNI CTCAG 6 cut(s) 19, 233, 298, 349, 547, 2134
BspHI TCATGA 1 cut(s) 2485
BspLI GGNNCC 9 cut(s) 129, 427, 428, 603, 1293, 1389, 1425, 2262, 2323
BspMAI CTGCAG 2 cut(s) 1921, 2167
BspMI ACCTGC 1 cut(s) 629
BspQI GCTCTTC 1 cut(s) 1845
BspT104I TTCGAA 1 cut(s) 165
BspT107I GGYRCC 1 cut(s) 1291
BsrDI GCAATG 1 cut(s) 195
BsrI ACTGG 6 cut(s) 1100, 1373, 1434, 1789, 2019, 2075
BssECI CCNNGG 1 cut(s) 1419
BssSI CACGAG 2 cut(s) 1128, 2348
Bst2BI CACGAG 2 cut(s) 1128, 2348
Bst2UI CCWGG 5 cut(s) 150, 654, 678, 732, 1956
Bst6I CTCTTC 3 cut(s) 65, 1765, 1845
BstAPI GCANNNNNTGC 3 cut(s) 958, 1204, 1213
BstBI TTCGAA 1 cut(s) 165
BstC8I GCNNGC 7 cut(s) 647, 963, 1307, 1905, 2213, 2481, 2545
BstDEI CTNAG 6 cut(s) 6, 220, 285, 336, 534, 2121
BstDSI CCRYGG 1 cut(s) 1419
BstMAI GTCTC 6 cut(s) 9, 239, 322, 573, 2192, 2426
BstNI CCWGG 5 cut(s) 150, 654, 678, 732, 1956
BstNSI RCATGY 5 cut(s) 86, 335, 1147, 1309, 1897
BstSCI CCNGG 7 cut(s) 148, 652, 676, 730, 1116, 1932, 1954
BstSFI CTRYAG 5 cut(s) 132, 633, 1828, 1917, 2163
BstSLI GKGCMC 1 cut(s) 1820
BstV2I GAAGAC 1 cut(s) 2493
BstX2I RGATCY 4 cut(s) 127, 601, 1604, 2260
BstXI CCANNNNNNTGG 2 cut(s) 653, 1000
BstYI RGATCY 4 cut(s) 127, 601, 1604, 2260
BsuRI GGCC 4 cut(s) 645, 853, 2456, 2543
BtgI CCRYGG 1 cut(s) 1419
BtsIMutI CAGTG 8 cut(s) 1107, 1380, 1782, 1818, 1992, 2010, 2062, 2292
BveI ACCTGC 1 cut(s) 629
Cac8I GCNNGC 7 cut(s) 647, 963, 1307, 1905, 2213, 2481, 2545
CaiI CAGNNNCTG 1 cut(s) 1213
CciI TCATGA 1 cut(s) 2485
Cfr13I GGNCC 4 cut(s) 426, 1388, 1424, 2029
CseI GACGC 1 cut(s) 2548
CsiI ACCWGGT 1 cut(s) 676
Csp6I GTAC 5 cut(s) 508, 672, 1082, 1520, 2068
CviQI GTAC 5 cut(s) 508, 672, 1082, 1520, 2068
DdeI CTNAG 6 cut(s) 6, 220, 285, 336, 534, 2121
DraIII CACNNNGTG 1 cut(s) 680
DrdI GACNNNNNNGTC 1 cut(s) 2558
DseDI GACNNNNNNGTC 1 cut(s) 2558
EaeI YGGCCR 3 cut(s) 643, 2454, 2541
Eam1104I CTCTTC 3 cut(s) 65, 1765, 1845
EarI CTCTTC 3 cut(s) 65, 1765, 1845
Eco24I GRGCYC 1 cut(s) 1550
Eco47I GGWCC 4 cut(s) 426, 1388, 1424, 2029
Eco57I CTGAAG 4 cut(s) 129, 875, 1681, 1746
EcoO109I RGGNCCY 3 cut(s) 426, 1388, 2029
EcoRII CCWGG 5 cut(s) 148, 652, 676, 730, 1954
EcoT38I GRGCYC 1 cut(s) 1550
FalI AAGNNNNNCTT 4 cut(s) 1701, 1733, 2016, 2048
FaqI GGGAC 7 cut(s) 200, 439, 901, 1420, 1437, 1995, 2241
FauI CCCGC 1 cut(s) 927
FbaI TGATCA 1 cut(s) 1330
FblI GTMKAC 1 cut(s) 1584
FriOI GRGCYC 1 cut(s) 1550
FspBI CTAG 7 cut(s) 201, 264, 1656, 1890, 2279, 2495, 2531
GsaI CCCAGC 2 cut(s) 433, 2260
GsuI CTGGAG 4 cut(s) 768, 1238, 2092, 2118
HaeIII GGCC 4 cut(s) 645, 853, 2456, 2543
HapII CCGG 3 cut(s) 1118, 1933, 2364
HgaI GACGC 1 cut(s) 2548
HincII GTYRAC 1 cut(s) 1414
HindII GTYRAC 1 cut(s) 1414
HindIII AAGCTT 1 cut(s) 524
HpaI GTTAAC 1 cut(s) 1414
HpaII CCGG 3 cut(s) 1118, 1933, 2364
HphI GGTGA 3 cut(s) 1089, 1431, 2428
Hpy166II GTNNAC 8 cut(s) 674, 1414, 1439, 1585, 1818, 1842, 2008, 2313
Hpy188III TCNNGA 6 cut(s) 419, 785, 2080, 2348, 2486, 2569
Hpy8I GTNNAC 8 cut(s) 674, 1414, 1439, 1585, 1818, 1842, 2008, 2313
Hpy99I CGWCG 1 cut(s) 1513
HpyAV CCTTC 9 cut(s) 104, 782, 990, 1005, 1048, 1452, 1461, 2042, 2274
HpyCH4IV ACGT 2 cut(s) 1875, 2381
HpyF3I CTNAG 6 cut(s) 6, 220, 285, 336, 534, 2121
HpySE526I ACGT 2 cut(s) 1875, 2381
KflI GGGWCCC 1 cut(s) 426
Ksp22I TGATCA 1 cut(s) 1330
KspAI GTTAAC 1 cut(s) 1414
LguI GCTCTTC 1 cut(s) 1845
LmnI GCTCC 3 cut(s) 268, 787, 2440
MabI ACCWGGT 1 cut(s) 676
MaeI CTAG 7 cut(s) 201, 264, 1656, 1890, 2279, 2495, 2531
MaeII ACGT 2 cut(s) 1875, 2381
MaeIII GTNAC 3 cut(s) 827, 1155, 2291
MfeI CAATTG 1 cut(s) 1551
MflI RGATCY 4 cut(s) 127, 601, 1604, 2260
MhlI GDGCHC 3 cut(s) 808, 1550, 1820
MlsI TGGCCA 1 cut(s) 645
MluNI TGGCCA 1 cut(s) 645
MlyI GAGTC 5 cut(s) 241, 598, 727, 832, 2467
MmeI TCCRAC 4 cut(s) 21, 554, 695, 2420
Mox20I TGGCCA 1 cut(s) 645
MroXI GAANNNNTTC 1 cut(s) 2343
MscI TGGCCA 1 cut(s) 645
MseI TTAA 5 cut(s) 521, 857, 1067, 1413, 2217
MslI CAYNNNNRTG 4 cut(s) 1148, 1644, 1997, 2484
Msp20I TGGCCA 1 cut(s) 645
MspA1I CMGCKG 3 cut(s) 230, 1466, 2328
MspI CCGG 3 cut(s) 1118, 1933, 2364
MspR9I CCNGG 7 cut(s) 150, 654, 678, 732, 1118, 1934, 1956
MunI CAATTG 1 cut(s) 1551
Mva1269I GAATGC 3 cut(s) 1026, 1855, 2245
MvaI CCWGG 5 cut(s) 150, 654, 678, 732, 1956
NciI CCSGG 2 cut(s) 1118, 1934
NlaIV GGNNCC 9 cut(s) 129, 427, 428, 603, 1293, 1389, 1425, 2262, 2323
NmeAIII GCCGAG 1 cut(s) 2482
NmuCI GTSAC 1 cut(s) 2291
NspI RCATGY 5 cut(s) 86, 335, 1147, 1309, 1897
NspV TTCGAA 1 cut(s) 165
OliI CACNNNNGTG 1 cut(s) 1997
PaeI GCATGC 1 cut(s) 1309
PagI TCATGA 1 cut(s) 2485
PaqCI CACCTGC 1 cut(s) 629
PciSI GCTCTTC 1 cut(s) 1845
PcsI WCGNNNNNNNCGW 1 cut(s) 2556
PctI GAATGC 3 cut(s) 1026, 1855, 2245
PdmI GAANNNNTTC 1 cut(s) 2343
PfeI GAWTC 7 cut(s) 455, 694, 766, 886, 1345, 1625, 2344
PfoI TCCNGGA 1 cut(s) 1954
PleI GAGTC 5 cut(s) 241, 597, 726, 832, 2467
PpsI GAGTC 5 cut(s) 241, 597, 726, 832, 2467
PpuMI RGGWCCY 3 cut(s) 426, 1388, 2029
Psp5II RGGWCCY 3 cut(s) 426, 1388, 2029
Psp6I CCWGG 5 cut(s) 148, 652, 676, 730, 1954
PspFI CCCAGC 2 cut(s) 429, 2256
PspGI CCWGG 5 cut(s) 148, 652, 676, 730, 1954
PspN4I GGNNCC 9 cut(s) 129, 427, 428, 603, 1293, 1389, 1425, 2262, 2323
PspPI GGNCC 4 cut(s) 426, 1388, 1424, 2029
PspPPI RGGWCCY 3 cut(s) 426, 1388, 2029
PstI CTGCAG 2 cut(s) 1921, 2167
PstNI CAGNNNCTG 1 cut(s) 1213
PsuI RGATCY 4 cut(s) 127, 601, 1604, 2260
PvuII CAGCTG 2 cut(s) 230, 2328
RsaI GTAC 5 cut(s) 509, 673, 1083, 1521, 2069
RsaNI GTAC 5 cut(s) 508, 672, 1082, 1520, 2068
RseI CAYNNNNRTG 4 cut(s) 1148, 1644, 1997, 2484
SapI GCTCTTC 1 cut(s) 1845
SaqAI TTAA 5 cut(s) 521, 857, 1067, 1413, 2217
Sau96I GGNCC 4 cut(s) 426, 1388, 1424, 2029
SchI GAGTC 5 cut(s) 241, 598, 727, 832, 2467
ScrFI CCNGG 7 cut(s) 150, 654, 678, 732, 1118, 1934, 1956
SduI GDGCHC 3 cut(s) 808, 1550, 1820
SexAI ACCWGGT 1 cut(s) 676
SfcI CTRYAG 5 cut(s) 132, 633, 1828, 1917, 2163
SfuI TTCGAA 1 cut(s) 165
SinI GGWCC 4 cut(s) 426, 1388, 1424, 2029
SmiMI CAYNNNNRTG 4 cut(s) 1148, 1644, 1997, 2484
SmlI CTYRAG 1 cut(s) 249
SmoI CTYRAG 1 cut(s) 249
SphI GCATGC 1 cut(s) 1309
SsiI CCGC 5 cut(s) 153, 920, 1466, 2065, 2510
SspMI CTAG 7 cut(s) 201, 264, 1656, 1890, 2279, 2495, 2531
StyD4I CCNGG 7 cut(s) 148, 652, 676, 730, 1116, 1932, 1954
TaiI ACGT 2 cut(s) 1878, 2384
TaqI TCGA 7 cut(s) 165, 418, 617, 877, 1036, 1511, 2305
TaqII GACCGA 1 cut(s) 1812
TatI WGTACW 1 cut(s) 1519
TauI GCSGC 1 cut(s) 156
TfiI GAWTC 7 cut(s) 455, 694, 766, 886, 1345, 1625, 2344
Tru1I TTAA 5 cut(s) 521, 857, 1067, 1413, 2217
Tru9I TTAA 5 cut(s) 521, 857, 1067, 1413, 2217
TscAI CASTG 8 cut(s) 1107, 1380, 1789, 1825, 1999, 2010, 2062, 2292
TseFI GTSAC 1 cut(s) 2291
Tsp45I GTSAC 1 cut(s) 2291
TspDTI ATGAA 7 cut(s) 83, 184, 425, 638, 779, 1166, 1278
TspGWI ACGGA 3 cut(s) 140, 825, 1721
TspRI CASTG 8 cut(s) 1107, 1380, 1789, 1825, 1999, 2010, 2062, 2292
VneI GTGCAC 1 cut(s) 1816
VpaK11BI GGWCC 4 cut(s) 426, 1388, 1424, 2029
XapI RAATTY 3 cut(s) 487, 973, 2372
XceI RCATGY 5 cut(s) 86, 335, 1147, 1309, 1897
XcmI CCANNNNNNNNNTGG 1 cut(s) 437
XmiI GTMKAC 1 cut(s) 1584
XmnI GAANNNNTTC 1 cut(s) 2343
XspI CTAG 7 cut(s) 201, 264, 1656, 1890, 2279, 2495, 2531
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.