MD15G1117100.v1.1

transcriptional co-repressor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0000670
Physical Location & Seq
Reverse (-)
8296345 .. 8304668
8324 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1117100.v1.1.491

Sequence Viewer

Length: 2700 bp
ATGTCTCATACCAACTGGGAAGCTGATAAAATGCTGGATGTGTATATCCATGATTATCTAGTGAAGCGGGATTTAAAAGCCTCTGCACAAGCTTTCCAAGCAGAAGGAAAAGTATCCTCAGATCCCGTTGCTATTGATGCGCCGGGAGGTTTCCTATTTGAATGGTGGTCGGTTTTCTGGGATATATTCATTGCTAGGACTAATGAGAAACATTCAGAGGTTGCGGCATCTTACATTGAGACTCAGTTGATTAAGGCAAGGGAGCAGCAACAGCAGCAGCAGCAACAACAACAGCAACAACATTCCCAGCAACCGCAAAACTCACAACAACAGCAGCAGCAAATGCAAATGCAGCAACTCATGATGCAAAGGCAACAACAGCAGCAGCAGCAGCAACAACAGCAGCAGCAGCAGCAGCAGCAGCAACAACAACAGCAGCAACAGCAGCAACCACAGCAGCAACAGCAACCGCCACAGCCGCAGCAGAGAAGAGATGGGGCACATCTTCTCAATGGAACTACAAATGGGCTTGTTGGAAATGATCCTCTCATGCGACAAAATCCTGGAACAGCGAATGCCATGGCTACAAAGATGTATGAGGAAAGATTAAAACTTCCTCTTCAGAGAGATGCTATGGATGATGCATCTATGAAGCAGAGATTTGGTGAAAATGTGGGCCAGATTTTGGATCAAAATCATGCTTCAATATTGAAGTCAGCTGCAGCAACCGGCCAGCCTTCAGGGCAAGTTTTGCATGGTACAGCTGGTGGGATGACTCAACAAGTCCAAGCTCGAAATCAGCAATTGGCAGGGTCTACACCGGACATAAAGACTGAAATCAATCCAGTATTGAATCCCAGAGCTGCAGGTCCTGAGGGATCATTAATAGGAATTCCTGGATCAAATCAGGGTGGAAACAATTTGACTTTAAAAGGATGGCCGCTCACAGGTTTGGAGCAGCTTCGCTCTGGACTTCTTCAGCAACAGAAACCTTTCATACAAGCTCCCCAGCCCTTTCATCAGCTGCAGATGTTGACACCACAACACCAGCAACTTATGCTTGCTCAGCAAAATATGACATCACCGTCTGCTGCCAACGATGAGAGTAGAAGACTAAGAATGCTATTGAATCGCGGTCCTGGGAAGGATGGCCTTGCAAATTCTGTTGGTGACGTGGTACCAAATATAGGATCACCTCTTCAAGCTGGTGGCCCTATTTTGCCTCGTGGAGATACAGATATGCTGATTAAGTTAAAAATGGCTCAACTTCAGCAGCAACAAAACAGCAATCCTCAACAGCAACAGCAACAGCAACAGCTTCAACAGCACGCTCTTTCTAATCAGCAGTCACAAAATTCAAATCTCAATCCCCATCAGCAAGATAAAATGGGGGGTGCTGGCAGCATCACTATGGATGGTAGCATGTCGAACTCTTTTCGAGGAAATGATCAGGTTTCAAAAAACCAGCCAGGGAGAAAGAGAAAGCAGCCAGTGTCATCTTCAGGGCCTGCCAATAGCACAGGAACAGCAAACACAGCTGGGCCTTCCCCGAGTTCAGCCCCCTCAACTCCCTCGACTCACACTCCTGGAGATGTAATCTCAATGCCTGCCTTGCCCCACAATGGTAGCTCCTCCAAGCCTCTGATGATGTTTGGTGCTGATGGTACTGGTACTCTTACATCACCATCACATCAGTTGGCTGATATGGATCGATTTGTAGAGGATGGGTCTCTTGATGATAATGTCGAGTCTTTTTTATCCCCTGATGATGTGGATCATAGAGATGCTGTTGGTCGATGTATGGATGTCAGCAAAGGGTTCACATTTACGGAAGTAAACTGTACTAGAGCAAGCGTAAGCAAGGTTACAAGCTGTCACTTCTCATCAGATGGAAAGTTTCTTGCTAGTGGGGGCCATGATAAAAAGGCTGTATTGTGGTACACAGATACATTAAAGCTAAAAAGTACGCTAGAAGAACATTCAGCTTTGATAACTGATGTTCGTTTTAGTCCGAGCATGCCACGTGTTGCAACGTCTTCATTCGACAAGACTGTCAGGGTTTGGGATGCCGACAATCCTGATTATTCTCTCCGTACCTTTATGGGACATTCGGCCTCTGTTATGTCATTAGATTTCCACCCAAATAAAGACGACCTCATCTGTTCTTGTGACGGGGATGGTCAGATACGCTACTGGAGTATTAACAAAGGCAGCTGCTCATGCGTGTCTAAGGGTGGTACGGCACAAATGAGATTTCAACCCCGTCTTGGAAGATTTCTTGCTGCAGCCGCAGAGAATGTTGTATCTATACTGGATGTTGAGACTCAGACTTGTCGGCATTCTTTACAGGGGCATACTAAGCCTATCCATTCTGTGTGTTGGGATCCTTCTGGCGAATTCCTTGCATCTGTCAGCGAGGACTCTGTAAGAGTTTGGACATTTGGAGCGGGAGGCGAAGGGGAATGCGTTCATGAGTTAAGCTGCAACGGAAATAAATTTCATTCATGCGTTTTCCATCCTACGTATACTTCACTGCTGGTCATTGGCTGTTATCAGTCACTGGAGTTATGGAACATGACAGAGAACAAGACAATGACTCTATCGGCACATGAAGGACTTATTGCTGCCTTGTCTGTGTCAACTGTTACGGGTTTGGTTGCTTCGGCTAGTCACGATAAGTTTGTCAAGCTCTGGAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0001101 GO:0001558 GO:0001666 GO:0003002 GO:0003006 GO:0005975 GO:0005976 GO:0006355 GO:0006950 GO:0006974 GO:0006979 GO:0007275 GO:0007389 GO:0008150 GO:0008152 GO:0009414 GO:0009415 GO:0009605 GO:0009607 GO:0009611 GO:0009617 GO:0009620 GO:0009624 GO:0009628 GO:0009636 GO:0009719 GO:0009725 GO:0009733 GO:0009791 GO:0009798 GO:0009845 GO:0009888 GO:0009889 GO:0009890 GO:0009892 GO:0009909 GO:0009943 GO:0009944 GO:0009955 GO:0009987 GO:0010033 GO:0010035 GO:0010038 GO:0010073 GO:0010154 GO:0010191 GO:0010214 GO:0010243 GO:0010272 GO:0010393 GO:0010468 GO:0010556 GO:0010558 GO:0010605 GO:0010629 GO:0014070 GO:0019219 GO:0019222 GO:0022414 GO:0022603 GO:0022604 GO:0030307 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0032501 GO:0032502 GO:0032504 GO:0033554 GO:0036293 GO:0040008 GO:0042221 GO:0042493 GO:0043170 GO:0043207 GO:0044237 GO:0044238 GO:0045892 GO:0045927 GO:0045934 GO:0045995 GO:0046677 GO:0046898 GO:0048316 GO:0048359 GO:0048507 GO:0048509 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048580 GO:0048608 GO:0048609 GO:0048638 GO:0048639 GO:0048731 GO:0048831 GO:0048856 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051094 GO:0051128 GO:0051130 GO:0051171 GO:0051172 GO:0051239 GO:0051252 GO:0051253 GO:0051510 GO:0051512 GO:0051704 GO:0051707 GO:0051716 GO:0060255 GO:0060992 GO:0061458 GO:0065001 GO:0065007 GO:0070482 GO:0071216 GO:0071217 GO:0071496 GO:0071704 GO:0080001 GO:0080090 GO:0090351 GO:0097305 GO:1901654 GO:1901698 GO:1901700 GO:1902074 GO:1902183 GO:1902679 GO:1903506 GO:1903507 GO:2000024 GO:2000026 GO:2000112 GO:2000113 GO:2000241 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LisH WD40

Protein Analysis

900

Amino Acids

98.71

Weight (kDa)

6.47

Isoelectric Point (pI)

52.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LisH PF08513 10 - 36 1.8e-06 LisH
WD40_Gbeta PF25391 607 - 744 8.4e-10 G protein beta WD-40 repeat protein
Beta-prop_CAF1B_HIR1 PF24105 609 - 693 1.4e-07 CAF1B/HIR1 beta-propeller domain
WD40_CDC20-Fz PF24807 609 - 675 9e-07 CDC20/Fizzy WD40 domain
Beta-prop_THOC3 PF25174 610 - 671 2.6e-07 THOC3 beta-propeller domain
WDR55 PF24796 612 - 744 2.5e-07 WDR55
Beta-prop_TEP1_2nd PF25047 613 - 732 3.4e-09 TEP-1 second beta-propeller
WD40_Prp19 PF24814 617 - 812 2.6e-31 Prp19 WD40 domain
Beta-prop_EML_2 PF23414 618 - 738 3.8e-14 Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_WDR3_1st PF25173 619 - 734 4.4e-20 WDR3 first beta-propeller domain
Beta-prop_THOC3 PF25174 620 - 692 8.8e-15 THOC3 beta-propeller domain
Beta-prop_WDR36-Utp21_2nd PF25168 620 - 719 3.9e-06 WDR36/Utp21 second beta-propeller domain
Beta-prop_WDR3_2nd PF25172 620 - 734 1.3e-13 WDR3 second beta-propeller domain
WD40_WDHD1_1st PF24817 620 - 692 8.4e-10 WDHD1 first WD40 domain
Beta-prop_WDR5 PF25175 620 - 740 1.2e-25 WDR5 beta-propeller domain
WD40_MABP1-WDR62_2nd PF24782 621 - 812 7.3e-12 MABP1/WDR62 second WD40 domain
Beta-prop_EML PF23409 643 - 740 2.1e-06 Echinoderm microtubule-associated protein first beta-propeller
WD40_CDC20-Fz PF24807 651 - 812 2e-17 CDC20/Fizzy WD40 domain
WD40 PF00400 652 - 689 4e-08 WD domain, G-beta repeat
Beta-prop_THOC3 PF25174 660 - 899 2.7e-36 THOC3 beta-propeller domain
WD40 PF00400 696 - 733 3e-06 WD domain, G-beta repeat
WD40_WDHD1_1st PF24817 698 - 769 1e-06 WDHD1 first WD40 domain
Beta-prop_WDR3_1st PF25173 699 - 858 2.7e-16 WDR3 first beta-propeller domain
Beta-prop_WDR3_2nd PF25172 699 - 813 1e-07 WDR3 second beta-propeller domain
WDR55 PF24796 704 - 869 1.2e-06 WDR55
Beta-prop_Aladin PF25460 705 - 809 3.1e-06 Aladin seven-bladed propeller
Beta-prop_WDR36-Utp21_2nd PF25168 721 - 815 8.9e-07 WDR36/Utp21 second beta-propeller domain
Beta-prop_WDR5 PF25175 746 - 898 9.6e-24 WDR5 beta-propeller domain
WD40_Gbeta PF25391 749 - 898 9.5e-09 G protein beta WD-40 repeat protein
WD40_Prp19 PF24814 750 - 898 1.6e-15 Prp19 WD40 domain
WD40_WDHD1_1st PF24817 758 - 899 2.9e-13 WDHD1 first WD40 domain
Beta-prop_WDR3_2nd PF25172 759 - 899 9.3e-09 WDR3 second beta-propeller domain
Beta-prop_WDR36-Utp21_1st PF25171 759 - 891 1.1e-07 WDR36/Utp21 first beta-propeller
WD40_CDC20-Fz PF24807 760 - 898 1.8e-07 CDC20/Fizzy WD40 domain
WD40 PF00400 777 - 812 6.5e-08 WD domain, G-beta repeat
Beta-prop_EIPR1 PF23609 780 - 898 5.6e-06 EIPR1 beta-propeller
Beta-prop_WDR3_1st PF25173 808 - 898 9.4e-06 WDR3 first beta-propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000670)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G32551 AT4G32551
fragaria_vesca FvH4_1g04170 FvH4_1g04170 FvH4_1g04170 FvH4_1g04170 FvH4_1g04170 FvH4_1g04170 FvH4_1g04170 FvH4_1g04170 FvH4_2g29940 FvH4_2g29940 FvH4_2g29940 FvH4_2g29940 FvH4_2g29940 FvH4_2g29940 FvH4_2g29940 FvH4_2g29940
malus_domestica MD02G1040200.v1.1 MD08G1139100.v1.1 MD15G1117100.v1.1 MD15G1145100.v1.1
prunus_persica Prupe.1G468400_v2.0.a1 Prupe.1G468400_v2.0.a1 Prupe.1G468400_v2.0.a1 Prupe.1G468400_v2.0.a1 Prupe.1G468400_v2.0.a1 Prupe.1G468400_v2.0.a1 Prupe.1G468400_v2.0.a1 Prupe.1G468400_v2.0.a1 Prupe.1G468400_v2.0.a1 Prupe.1G468400_v2.0.a1 Prupe.1G468400_v2.0.a1 Prupe.1G468400_v2.0.a1 Prupe.1G468400_v2.0.a1 Prupe.1G468400_v2.0.a1 Prupe.1G468400_v2.0.a1 Prupe.1G468400_v2.0.a1 Prupe.1G468400_v2.0.a1 Prupe.7G235500_v2.0.a1 Prupe.7G235500_v2.0.a1 Prupe.7G235500_v2.0.a1 Prupe.7G235500_v2.0.a1
pyrus_communis pycom02g03350 pycom08g11750 pycom15g10550 pycom15g16250
rosa_chinensis RchiOBHm_Chr2g0089671 RchiOBHm_Chr3g0465211 RchiOBHm_Chr6g0312941
rosa_laevigata RLG00000010283 RLG00000016050
rosa_multiflora Rmu_sc0000665.1_g000023 Rmu_sc0008049.1_g000043 Rmu_sc0008049.1_g000044
rosa_roxburghii Rroxscaffold_2G00151660 Rroxscaffold_7G00156310
rosa_rugosa Rorug02G0002500 Rorug02G0002500 Rorug06G0400700 Rorug06G0400700 Rorug06G0400700 Rorug06G0400700 Rorug06G0400800.1 Rorug06G0400900.1
rosa_samantha Rh2AG047900 Rh2BG046500 Rh2CG048500 Rh2DG047900 Rh6AG517700 Rh6BG529400 Rh6CG533900 Rh6DG520500
rosa_wichuraiana Rw2G004260 Rw5G017770 Rw6G045010

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 1084, 2051
Acc36I ACCTGC 1 cut(s) 857
Acc65I GGTACC 1 cut(s) 1177
AccB1I GGYRCC 1 cut(s) 1177
AccB7I CCANNNNNTGG 1 cut(s) 685
AccBSI CCGCTC 2 cut(s) 943, 2447
AccI GTMKAC 2 cut(s) 815, 2525
AccII CGCG 1 cut(s) 1134
AciI CCGC 9 cut(s) 67, 224, 314, 470, 479, 941, 1134, 2289, 2447
AcoI YGGCCR 2 cut(s) 730, 938
AcsI RAATTY 5 cut(s) 891, 1159, 1354, 2396, 2495
AcuI CTGAAG 5 cut(s) 605, 723, 962, 1253, 1485
AcvI CACGTG 1 cut(s) 2024
AfaI GTAC 9 cut(s) 760, 1179, 1666, 1672, 1843, 1940, 1966, 2095, 2239
AfiI CCNNNNNNNGG 5 cut(s) 685, 947, 1187, 1622, 2267
AflIII ACRYGT 1 cut(s) 2023
AjiI CACGTC 1 cut(s) 1174
AjnI CCWGG 5 cut(s) 562, 895, 1138, 1468, 1585
Alw26I GTCTC 4 cut(s) 9, 233, 1734, 2315
AlwNI CAGNNNCTG 3 cut(s) 872, 1508, 2560
Ama87I CYCGRG 1 cut(s) 1549
AoxI GGCC 9 cut(s) 676, 730, 938, 1150, 1210, 1505, 1541, 1912, 2112
ApoI RAATTY 5 cut(s) 891, 1159, 1354, 2396, 2495
AseI ATTAAT 1 cut(s) 884
Asp700I GAANNNNTTC 2 cut(s) 992, 2466
Asp718I GGTACC 1 cut(s) 1177
AspLEI GCGC 1 cut(s) 142
AspS9I GGNCC 7 cut(s) 676, 869, 1136, 1211, 1505, 1541, 1912
AsuC2I CCSGG 1 cut(s) 144
AsuHPI GGTGA 5 cut(s) 677, 1074, 1181, 1185, 1674
AvaI CYCGRG 1 cut(s) 1549
AvaII GGWCC 2 cut(s) 869, 1136
AxyI CCTNAGG 1 cut(s) 873
BaeGI GKGCMC 1 cut(s) 502
BamHI GGATCC 1 cut(s) 2383
BanI GGYRCC 1 cut(s) 1177
BauI CACGAG 1 cut(s) 1224
BbrPI CACGTG 1 cut(s) 2024
BbsI GAAGAC 2 cut(s) 1117, 2028
BceAI ACGGC 1 cut(s) 2256
BcgI CGANNNNNNTGC 2 cut(s) 2384, 2418
BciT130I CCWGG 5 cut(s) 564, 897, 1140, 1470, 1587
BciVI GTATCC 1 cut(s) 124
BclI TGATCA 1 cut(s) 1447
BcnI CCSGG 1 cut(s) 144
BcoDI GTCTC 4 cut(s) 9, 233, 1734, 2315
BfaI CTAG 6 cut(s) 59, 195, 1845, 1905, 1970, 2667
BfmI CTRYAG 4 cut(s) 720, 864, 1025, 2283
BfuAI ACCTGC 1 cut(s) 857
BfuI GTATCC 1 cut(s) 124
BglI GCCNNNNNGGC 1 cut(s) 742
BlpI GCTNAGC 1 cut(s) 1065
Bme1390I CCNGG 6 cut(s) 144, 564, 897, 1140, 1470, 1587
Bme18I GGWCC 2 cut(s) 869, 1136
BmeT110I CYCGRG 1 cut(s) 1549
BmgBI CACGTC 1 cut(s) 1174
BmgT120I GGNCC 7 cut(s) 676, 869, 1136, 1211, 1505, 1541, 1912
BmiI GGNNCC 3 cut(s) 1179, 1913, 2385
BmrFI CCNGG 6 cut(s) 144, 564, 897, 1140, 1470, 1587
BmrI ACTGGG 1 cut(s) 25
BmuI ACTGGG 1 cut(s) 25
BpiI GAAGAC 2 cut(s) 1117, 2028
BpmI CTGGAG 3 cut(s) 1608, 2215, 2582
Bpu1102I GCTNAGC 1 cut(s) 1065
BpuMI CCSGG 1 cut(s) 144
Bsa29I ATCGAT 1 cut(s) 1711
BsaAI YACGTR 2 cut(s) 2024, 2523
BsaBI GATNNNNATC 3 cut(s) 693, 1707, 1773
BsaI GGTCTC 1 cut(s) 1734
BsaJI CCNNGG 3 cut(s) 579, 1139, 1469
BsaWI WCCGGW 1 cut(s) 820
BsaXI ACNNNNNCTCC 2 cut(s) 1567, 1597
Bsc4I CCNNNNNNNGG 5 cut(s) 685, 947, 1187, 1622, 2267
Bse118I RCCGGY 1 cut(s) 728
Bse1I ACTGG 7 cut(s) 20, 845, 1490, 1672, 2198, 2316, 2565
Bse21I CCTNAGG 1 cut(s) 873
Bse3DI GCAATG 1 cut(s) 189
Bse8I GATNNNNATC 3 cut(s) 693, 1707, 1773
BseBI CCWGG 5 cut(s) 564, 897, 1140, 1470, 1587
BseCI ATCGAT 1 cut(s) 1711
BseDI CCNNGG 3 cut(s) 579, 1139, 1469
BseJI GATNNNNATC 3 cut(s) 693, 1707, 1773
BseLI CCNNNNNNNGG 5 cut(s) 685, 947, 1187, 1622, 2267
BseMI GCAATG 1 cut(s) 189
BseMII CTCAG 5 cut(s) 132, 257, 864, 1079, 2339
BseNI ACTGG 7 cut(s) 20, 845, 1490, 1672, 2198, 2316, 2565
BseRI GAGGAG 1 cut(s) 1621
BseSI GKGCMC 1 cut(s) 502
BseYI CCCAGC 3 cut(s) 306, 1008, 1538
BsgI GTGCAG 1 cut(s) 69
Bsh1236I CGCG 1 cut(s) 1134
BshFI GGCC 9 cut(s) 678, 732, 940, 1152, 1212, 1507, 1543, 1914, 2114
BshNI GGYRCC 1 cut(s) 1177
BshVI ATCGAT 1 cut(s) 1711
BsiHKCI CYCGRG 1 cut(s) 1549
BsiSI CCGG 3 cut(s) 143, 729, 821
BslFI GGGAC 1 cut(s) 2118
BslI CCNNNNNNNGG 5 cut(s) 685, 947, 1187, 1622, 2267
BsmAI GTCTC 4 cut(s) 9, 233, 1734, 2315
BsmFI GGGAC 1 cut(s) 2118
BsmI GAATGC 4 cut(s) 580, 1125, 2338, 2468
BsnI GGCC 9 cut(s) 678, 732, 940, 1152, 1212, 1507, 1543, 1914, 2114
Bso31I GGTCTC 1 cut(s) 1734
BsoBI CYCGRG 1 cut(s) 1549
Bsp1286I GDGCHC 1 cut(s) 502
Bsp1720I GCTNAGC 1 cut(s) 1065
Bsp19I CCATGG 1 cut(s) 579
BspACI CCGC 9 cut(s) 67, 224, 314, 470, 479, 941, 1134, 2289, 2447
BspANI GGCC 9 cut(s) 678, 732, 940, 1152, 1212, 1507, 1543, 1914, 2114
BspCNI CTCAG 5 cut(s) 131, 256, 865, 1078, 2338
BspDI ATCGAT 1 cut(s) 1711
BspFNI CGCG 1 cut(s) 1134
BspHI TCATGA 2 cut(s) 360, 2470
BspLI GGNNCC 3 cut(s) 1179, 1913, 2385
BspMAI CTGCAG 4 cut(s) 724, 868, 1029, 2287
BspMI ACCTGC 1 cut(s) 857
BspT107I GGYRCC 1 cut(s) 1177
BspTNI GGTCTC 1 cut(s) 1734
BsrBI CCGCTC 2 cut(s) 943, 2447
BsrDI GCAATG 1 cut(s) 189
BsrFI RCCGGY 1 cut(s) 728
BsrI ACTGG 7 cut(s) 20, 845, 1490, 1672, 2198, 2316, 2565
BssAI RCCGGY 1 cut(s) 728
BssECI CCNNGG 3 cut(s) 579, 1139, 1469
BssNAI GTATAC 1 cut(s) 2526
BssSI CACGAG 1 cut(s) 1224
BssT1I CCWWGG 1 cut(s) 579
Bst1107I GTATAC 1 cut(s) 2526
Bst2BI CACGAG 1 cut(s) 1224
Bst2UI CCWGG 5 cut(s) 564, 897, 1140, 1470, 1587
Bst4CI ACNGT 4 cut(s) 1086, 1841, 2053, 2644
Bst6I CTCTTC 3 cut(s) 484, 624, 1203
BstAPI GCANNNNNTGC 3 cut(s) 343, 751, 1057
BstBAI YACGTR 2 cut(s) 2024, 2523
BstC8I GCNNGC 8 cut(s) 734, 1062, 1329, 1399, 1509, 1608, 1852, 2018
BstDEI CTNAG 8 cut(s) 118, 243, 873, 1065, 1115, 2229, 2325, 2358
BstDSI CCRYGG 1 cut(s) 579
BstFNI CGCG 1 cut(s) 1134
BstHHI GCGC 1 cut(s) 142
BstMAI GTCTC 4 cut(s) 9, 233, 1734, 2315
BstNI CCWGG 5 cut(s) 564, 897, 1140, 1470, 1587
BstNSI RCATGY 2 cut(s) 1426, 2020
BstSCI CCNGG 6 cut(s) 142, 562, 895, 1138, 1468, 1585
BstSFI CTRYAG 4 cut(s) 720, 864, 1025, 2283
BstSLI GKGCMC 1 cut(s) 502
BstSNI TACGTA 1 cut(s) 2523
BstUI CGCG 1 cut(s) 1134
BstV2I GAAGAC 2 cut(s) 1117, 2028
BstX2I RGATCY 2 cut(s) 121, 2383
BstYI RGATCY 2 cut(s) 121, 2383
BstZ17I GTATAC 1 cut(s) 2526
Bsu15I ATCGAT 1 cut(s) 1711
Bsu36I CCTNAGG 1 cut(s) 873
BsuI GTATCC 1 cut(s) 124
BsuRI GGCC 9 cut(s) 678, 732, 940, 1152, 1212, 1507, 1543, 1914, 2114
BsuTUI ATCGAT 1 cut(s) 1711
BtgI CCRYGG 1 cut(s) 579
BtrI CACGTC 1 cut(s) 1174
BtsI GCAGTG 1 cut(s) 2531
BtsIMutI CAGTG 3 cut(s) 1497, 2531, 2558
BveI ACCTGC 1 cut(s) 857
Cac8I GCNNGC 8 cut(s) 734, 1062, 1329, 1399, 1509, 1608, 1852, 2018
CaiI CAGNNNCTG 3 cut(s) 872, 1508, 2560
CciI TCATGA 2 cut(s) 360, 2470
CfoI GCGC 1 cut(s) 142
Cfr10I RCCGGY 1 cut(s) 728
Cfr13I GGNCC 7 cut(s) 676, 869, 1136, 1211, 1505, 1541, 1912
ClaI ATCGAT 1 cut(s) 1711
Csp6I GTAC 9 cut(s) 759, 1178, 1665, 1671, 1842, 1939, 1965, 2094, 2238
CviQI GTAC 9 cut(s) 759, 1178, 1665, 1671, 1842, 1939, 1965, 2094, 2238
DdeI CTNAG 8 cut(s) 118, 243, 873, 1065, 1115, 2229, 2325, 2358
DraI TTTAAA 2 cut(s) 75, 930
DrdI GACNNNNNNGTC 2 cut(s) 1084, 2051
DseDI GACNNNNNNGTC 2 cut(s) 1084, 2051
EaeI YGGCCR 2 cut(s) 730, 938
Eam1104I CTCTTC 3 cut(s) 484, 624, 1203
EarI CTCTTC 3 cut(s) 484, 624, 1203
Eco105I TACGTA 1 cut(s) 2523
Eco130I CCWWGG 1 cut(s) 579
Eco31I GGTCTC 1 cut(s) 1734
Eco47I GGWCC 2 cut(s) 869, 1136
Eco57I CTGAAG 5 cut(s) 605, 723, 962, 1253, 1485
Eco72I CACGTG 1 cut(s) 2024
Eco81I CCTNAGG 1 cut(s) 873
Eco88I CYCGRG 1 cut(s) 1549
EcoO109I RGGNCCY 2 cut(s) 869, 1505
EcoRI GAATTC 2 cut(s) 891, 2396
EcoRII CCWGG 5 cut(s) 562, 895, 1138, 1468, 1585
EcoT14I CCWWGG 1 cut(s) 579
EcoT22I ATGCAT 1 cut(s) 646
ErhI CCWWGG 1 cut(s) 579
FaqI GGGAC 1 cut(s) 2118
FauI CCCGC 2 cut(s) 60, 2440
FbaI TGATCA 1 cut(s) 1447
FblI GTMKAC 2 cut(s) 815, 2525
FspBI CTAG 6 cut(s) 59, 195, 1845, 1905, 1970, 2667
GlaI GCGC 1 cut(s) 141
GsaI CCCAGC 3 cut(s) 310, 1012, 1542
GsuI CTGGAG 3 cut(s) 1608, 2215, 2582
HaeIII GGCC 9 cut(s) 678, 732, 940, 1152, 1212, 1507, 1543, 1914, 2114
HapII CCGG 3 cut(s) 143, 729, 821
HhaI GCGC 1 cut(s) 142
Hin6I GCGC 1 cut(s) 140
HinP1I GCGC 1 cut(s) 140
HincII GTYRAC 2 cut(s) 1035, 2640
HindII GTYRAC 2 cut(s) 1035, 2640
HindIII AAGCTT 1 cut(s) 90
HinfI GANTC 9 cut(s) 241, 775, 853, 1129, 1576, 1748, 2323, 2420, 2596
HpaII CCGG 3 cut(s) 143, 729, 821
HphI GGTGA 5 cut(s) 677, 1074, 1181, 1185, 1674
Hpy166II GTNNAC 7 cut(s) 816, 1035, 1821, 1837, 1941, 2526, 2640
Hpy188I TCNGA 8 cut(s) 121, 217, 624, 1644, 1888, 2013, 2184, 2328
Hpy188III TCNNGA 8 cut(s) 361, 872, 969, 1733, 2078, 2471, 2672, 2692
Hpy8I GTNNAC 7 cut(s) 816, 1035, 1821, 1837, 1941, 2526, 2640
HpyAV CCTTC 7 cut(s) 98, 747, 1138, 1554, 2397, 2450, 2606
HpyCH4III ACNGT 4 cut(s) 1086, 1841, 2053, 2644
HpyCH4IV ACGT 4 cut(s) 1173, 2023, 2033, 2522
HpyF3I CTNAG 8 cut(s) 118, 243, 873, 1065, 1115, 2229, 2325, 2358
HpySE526I ACGT 4 cut(s) 1173, 2023, 2033, 2522
HspAI GCGC 1 cut(s) 140
KpnI GGTACC 1 cut(s) 1181
Ksp22I TGATCA 1 cut(s) 1447
LmnI GCTCC 5 cut(s) 262, 955, 1009, 1634, 2444
MaeI CTAG 6 cut(s) 59, 195, 1845, 1905, 1970, 2667
MaeII ACGT 4 cut(s) 1173, 2023, 2033, 2522
MaeIII GTNAC 8 cut(s) 1169, 1347, 1864, 1874, 2168, 2556, 2644, 2669
MbiI CCGCTC 2 cut(s) 943, 2447
MfeI CAATTG 1 cut(s) 803
MflI RGATCY 2 cut(s) 121, 2383
MhlI GDGCHC 1 cut(s) 502
MluCI AATT 7 cut(s) 803, 891, 919, 1159, 1354, 2396, 2495
MlyI GAGTC 7 cut(s) 235, 769, 1570, 1757, 2317, 2414, 2590
MmeI TCCRAC 1 cut(s) 514
Mph1103I ATGCAT 1 cut(s) 646
MroXI GAANNNNTTC 2 cut(s) 992, 2466
MslI CAYNNNNRTG 2 cut(s) 1409, 1782
MspA1I CMGCKG 5 cut(s) 719, 764, 1024, 1538, 2214
MspI CCGG 3 cut(s) 143, 729, 821
MspR9I CCNGG 6 cut(s) 144, 564, 897, 1140, 1470, 1587
MunI CAATTG 1 cut(s) 803
Mva1269I GAATGC 4 cut(s) 580, 1125, 2338, 2468
MvaI CCWGG 5 cut(s) 564, 897, 1140, 1470, 1587
MvnI CGCG 1 cut(s) 1134
NciI CCSGG 1 cut(s) 144
NcoI CCATGG 1 cut(s) 579
NlaIV GGNNCC 3 cut(s) 1179, 1913, 2385
NmuCI GTSAC 6 cut(s) 1169, 1347, 1874, 2168, 2556, 2669
NsiI ATGCAT 1 cut(s) 646
NspI RCATGY 2 cut(s) 1426, 2020
PaeI GCATGC 1 cut(s) 2020
PagI TCATGA 2 cut(s) 360, 2470
PctI GAATGC 4 cut(s) 580, 1125, 2338, 2468
PdmI GAANNNNTTC 2 cut(s) 992, 2466
PfeI GAWTC 2 cut(s) 853, 1129
PflMI CCANNNNNTGG 1 cut(s) 685
PfoI TCCNGGA 3 cut(s) 562, 895, 1585
PleI GAGTC 7 cut(s) 235, 769, 1570, 1756, 2317, 2414, 2590
PmaCI CACGTG 1 cut(s) 2024
PmlI CACGTG 1 cut(s) 2024
PpsI GAGTC 7 cut(s) 235, 769, 1570, 1756, 2317, 2414, 2590
Ppu21I YACGTR 2 cut(s) 2024, 2523
PpuMI RGGWCCY 1 cut(s) 869
PshBI ATTAAT 1 cut(s) 884
Psp5II RGGWCCY 1 cut(s) 869
Psp6I CCWGG 5 cut(s) 562, 895, 1138, 1468, 1585
PspCI CACGTG 1 cut(s) 2024
PspFI CCCAGC 3 cut(s) 306, 1008, 1538
PspGI CCWGG 5 cut(s) 562, 895, 1138, 1468, 1585
PspN4I GGNNCC 3 cut(s) 1179, 1913, 2385
PspPI GGNCC 7 cut(s) 676, 869, 1136, 1211, 1505, 1541, 1912
PspPPI RGGWCCY 1 cut(s) 869
PstI CTGCAG 4 cut(s) 724, 868, 1029, 2287
PstNI CAGNNNCTG 3 cut(s) 872, 1508, 2560
PsuI RGATCY 2 cut(s) 121, 2383
PvuII CAGCTG 5 cut(s) 719, 764, 1024, 1538, 2214
RsaI GTAC 9 cut(s) 760, 1179, 1666, 1672, 1843, 1940, 1966, 2095, 2239
RsaNI GTAC 9 cut(s) 759, 1178, 1665, 1671, 1842, 1939, 1965, 2094, 2238
RseI CAYNNNNRTG 2 cut(s) 1409, 1782
Sau96I GGNCC 7 cut(s) 676, 869, 1136, 1211, 1505, 1541, 1912
SchI GAGTC 7 cut(s) 235, 769, 1570, 1757, 2317, 2414, 2590
ScrFI CCNGG 6 cut(s) 144, 564, 897, 1140, 1470, 1587
SduI GDGCHC 1 cut(s) 502
SfcI CTRYAG 4 cut(s) 720, 864, 1025, 2283
SinI GGWCC 2 cut(s) 869, 1136
SmiMI CAYNNNNRTG 2 cut(s) 1409, 1782
SnaBI TACGTA 1 cut(s) 2523
SphI GCATGC 1 cut(s) 2020
Sse9I AATT 7 cut(s) 803, 891, 919, 1159, 1354, 2396, 2495
SsiI CCGC 9 cut(s) 67, 224, 314, 470, 479, 941, 1134, 2289, 2447
SspI AATATT 1 cut(s) 708
SspMI CTAG 6 cut(s) 59, 195, 1845, 1905, 1970, 2667
StyD4I CCNGG 6 cut(s) 142, 562, 895, 1138, 1468, 1585
StyI CCWWGG 1 cut(s) 579
TaaI ACNGT 4 cut(s) 1086, 1841, 2053, 2644
TaiI ACGT 4 cut(s) 1176, 2026, 2036, 2525
TaqI TCGA 8 cut(s) 793, 1427, 1438, 1574, 1711, 1746, 1795, 2043
TasI AATT 7 cut(s) 803, 891, 919, 1159, 1354, 2396, 2495
TatI WGTACW 1 cut(s) 1841
TauI GCSGC 4 cut(s) 227, 481, 943, 2291
TfiI GAWTC 2 cut(s) 853, 1129
TscAI CASTG 3 cut(s) 1497, 2538, 2565
TseFI GTSAC 6 cut(s) 1169, 1347, 1874, 2168, 2556, 2669
Tsp45I GTSAC 6 cut(s) 1169, 1347, 1874, 2168, 2556, 2669
TspDTI ATGAA 9 cut(s) 178, 665, 985, 1007, 2028, 2459, 2489, 2493, 2625
TspGWI ACGGA 3 cut(s) 1844, 2081, 2502
TspRI CASTG 3 cut(s) 1497, 2538, 2565
Van91I CCANNNNNTGG 1 cut(s) 685
VpaK11BI GGWCC 2 cut(s) 869, 1136
VspI ATTAAT 1 cut(s) 884
XapI RAATTY 5 cut(s) 891, 1159, 1354, 2396, 2495
XceI RCATGY 2 cut(s) 1426, 2020
XmiI GTMKAC 2 cut(s) 815, 2525
XmnI GAANNNNTTC 2 cut(s) 992, 2466
XspI CTAG 6 cut(s) 59, 195, 1845, 1905, 1970, 2667
Zsp2I ATGCAT 1 cut(s) 646
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.