MD08G1139100.v1.1

transcriptional co-repressor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0000670
Physical Location & Seq
Reverse (-)
13415661 .. 13424078
8418 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1139100.v1.1.491

Sequence Viewer

Length: 2700 bp
ATGTCTCAGACCAACTGGGAAGCTGATAAAATGCTGGATGTGTATATCCATGATTATTTAGTGAAGAGGGATTTAAAAGCTTCTGCACAAGCTTTCCAAGCTGAAGGAAAAGTATCCTCAGATCCGGTTGCTATTGATGCACCAGGAGGTTTCCTGTTTGAATGGTGGTCGGTTTTCTGGGATATATTCATTGCTAGGACTAATGAGAAGCATTCAGAGGTTGCGGCATCGTACATTGAGACTCAGTTGATTAAAGCAAGGGAGCAGCAACAGCAGCAGCAGCAACAACAACAACAACAACAACAGCAACATTCCCAGCAACCGCAAAACTCACAACAACAGCAGCAGCAGCTGCAAATGCAGCAACTCATGTTGCAAAGGCATGTTCAGCAGCAGCAGCAGCAACAACAACAACAACAGCAGCAGCAGCAGCAGCAACAGCAGCAACCACAGCAGCAACAGCAACCGCCACAGTCGCATCAGAGAAGAGATGGGGCACATCTCCACAATGGACCTACAAATGGGCTTGTTGGAAATGATCCTCTCATGCGACAAAATCCTGGAACAGCAAATGCCATGGCTTCAAAGATGTATGAGGAAAGATTAAAACTTCCTCTTCAGAGAGATTCTATGGATGATGCATCTATGAAACAGAGATTTGGTGATAATGTGGGCCAGATTTTGGATCAAAATCATGCTTCAATATTGAAGTCAGCCGCAGCAACCGGCCAGCCTTCAGGGCAAGTTTTGCACGGTACAGCTGGTGGGATGACTCAACAAGTCCAAGCTCGAAATCAGCAATTGCCAGGGTCTACACCGGACATAAAGACTGAAATCAATCCAGTATTGAATCCCAGAGCTGCATGTCCTGAGGGATCATTGATAGGAATTCCTGGATCAAATCAGGGTGGAAACAACTTGACTTTAAAAGGATGGCCGCTCACTGGTTTGGAGCAGCTTCGCTCTGGACTTCTTCAGCAACAGAAACCTTTCATACAAGCTCCCCAGCCCTTTCATCAGCTTCAGATGTTGACACCACAACACCAGCAACTTATGCTTGCTCAGCAAAATATGACATCACCGTCTGCTGCCAGTGATGAAAGTAGACGACTAAGAATGCTAATGAGTCGCAGTCTTGGGAAGGATGGCCTAACAAATTCTGTTGGTGACGTGGTACCAAATATGGGATCACCTCTTCAAGCTGGTGGCCCTATTTTGCCTCGTGGAGACACAGATATGCTGATTAAGGTAAAAATGGCTCAACTACAGCAGCAACAAAACAGCAATCCCCAACAGCAGCAGCAACAGCTTCAACAACTTCAACAGCATGGTCTTTCTAATCAGCAGTCGCAAAATTCAAATCTCAATCCCCATCAGCAAGATAAAATGGGGGGTGCTGGCAGCATCACTATGGATGGTAGCATGTCAAACTCTTTTCGAGGAAATGATCAGGTTTCAAAAAACCAGGCAGGAAGAAAGAGAAAGCAGCCAGTGTCATCTTCAGGGCCTGCCAATAGCACAGGTACAGCAAACACAGCTGGGCCTTCCCCGAGTTCAGCCCCTTCAACTCCCTCGACTCACACTCCTGGAGATGTAATCTCAATGCCTGCCTTGCCCCATAGTGGTAGCTCCTCCAAGCCTCTGATGATGTTTGGCGCTGATGGTACTGGTACTCTTACTTCACCAGCACATCAGTTGGCTGATATGGACCGATTTGTAGAGGATGGGTCTCTTGATGATAATGTCGAGTCTTTTTTATCCCCTGATGATGTGGATCATAGAGATGCTGTTGGTCGATGTATGGATGTCAGCAAAGGGTTCGCATTTACGGAAGTAAACTCTATTAGAGCAAGCGCAAGCAAGGTTACAAGCTGTCACTTCTCATCAGATGGAAAGTTTCTTGCTAGTGGGGGCCATGATAAGAAGGCTGTTTTATGGTACACAGATACATTAAAGCCAAAAAGTACGCTAGAAGAACATTCAGCTTTGATAACTGATGTTCGTTTTAGTCCGAGCATGCCACGTCTTGCAACATCTTCATTCGACAAGACTGTCAGGGTGTGGGATGCTGACAATCCTGGTTATTCTCTCCGTACCTTTATGGGACATTCGGCCTCTGTTATGTCATTAGATTTCCACCCAAATAAAGATGACCTCATCTGTTCTTGTGACAGTGATGGTCAGATACGCTACTGGAGCATTAACAATGGCAGCTGCTCGAGTGTGTTCAAGGGTGGTACAGCACAAATGAGATTTCAACCCCGTCTTGGAAGATTGCTTGCTGCAGCCGCTGAGAATGTTGTGTCTATACTGGATGTCGAGACTCAGACTTGTCGACATTCATTACAGGGGCATACTAAGCCTATCCATTCTGTGTGTTGGGATCCTTCTGGTGAGTTCCTTGCATCTGTCAGCGAGGACTCTGTAAGAGTTTGGACACTTGGAGCTGGAGGCGAAGGGGAATGCGTTCATGAGTTGAGCTGCAACGGAAATAAATTTCATTCATGCGTTTTCCATCCTACATATACTTCACTGCTGGTCATTGGCTGTTATCAGTCATTGGAGTTATGGAACATGACGGAGAACAAGACGATGACTCTATCAGCACACGATGGACTTATTGCTGCATTGTCTGTGTCAACCGGTACTGGTTTGGTTGCTTCGGCTAGTCACGACAAGTTTGTCAAGCTCTGGAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0001101 GO:0001558 GO:0001666 GO:0003002 GO:0003006 GO:0005975 GO:0005976 GO:0006355 GO:0006950 GO:0006974 GO:0006979 GO:0007275 GO:0007389 GO:0008150 GO:0008152 GO:0009414 GO:0009415 GO:0009605 GO:0009607 GO:0009611 GO:0009617 GO:0009620 GO:0009624 GO:0009628 GO:0009636 GO:0009719 GO:0009725 GO:0009733 GO:0009791 GO:0009798 GO:0009845 GO:0009888 GO:0009889 GO:0009890 GO:0009892 GO:0009909 GO:0009943 GO:0009944 GO:0009955 GO:0009987 GO:0010033 GO:0010035 GO:0010038 GO:0010073 GO:0010154 GO:0010191 GO:0010214 GO:0010243 GO:0010272 GO:0010393 GO:0010468 GO:0010556 GO:0010558 GO:0010605 GO:0010629 GO:0014070 GO:0019219 GO:0019222 GO:0022414 GO:0022603 GO:0022604 GO:0030307 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0032501 GO:0032502 GO:0032504 GO:0033554 GO:0036293 GO:0040008 GO:0042221 GO:0042493 GO:0043170 GO:0043207 GO:0044237 GO:0044238 GO:0045892 GO:0045927 GO:0045934 GO:0045995 GO:0046677 GO:0046898 GO:0048316 GO:0048359 GO:0048507 GO:0048509 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048580 GO:0048608 GO:0048609 GO:0048638 GO:0048639 GO:0048731 GO:0048831 GO:0048856 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051094 GO:0051128 GO:0051130 GO:0051171 GO:0051172 GO:0051239 GO:0051252 GO:0051253 GO:0051510 GO:0051512 GO:0051704 GO:0051707 GO:0051716 GO:0060255 GO:0060992 GO:0061458 GO:0065001 GO:0065007 GO:0070482 GO:0071216 GO:0071217 GO:0071496 GO:0071704 GO:0080001 GO:0080090 GO:0090351 GO:0097305 GO:1901654 GO:1901698 GO:1901700 GO:1902074 GO:1902183 GO:1902679 GO:1903506 GO:1903507 GO:2000024 GO:2000026 GO:2000112 GO:2000113 GO:2000241 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LisH WD40

Protein Analysis

900

Amino Acids

98.49

Weight (kDa)

6.51

Isoelectric Point (pI)

53.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LisH PF08513 10 - 36 1.8e-06 LisH
WD40_Gbeta PF25391 607 - 748 5.7e-12 G protein beta WD-40 repeat protein
Beta-prop_THOC3 PF25174 608 - 671 6.7e-08 THOC3 beta-propeller domain
WD40_MABP1-WDR62_2nd PF24782 609 - 814 7.2e-13 MABP1/WDR62 second WD40 domain
WD40_CDC20-Fz PF24807 609 - 675 3.4e-08 CDC20/Fizzy WD40 domain
Beta-prop_CAF1B_HIR1 PF24105 610 - 692 4.2e-07 CAF1B/HIR1 beta-propeller domain
WD40 PF00400 612 - 646 4.2e-06 WD domain, G-beta repeat
Beta-prop_WDR3_1st PF25173 616 - 724 3.6e-18 WDR3 first beta-propeller domain
Beta-prop_WDR5 PF25175 617 - 744 1.1e-26 WDR5 beta-propeller domain
Beta-prop_EML_2 PF23414 618 - 738 3.8e-15 Echinoderm microtubule-associated protein second beta-propeller
WD40_Prp19 PF24814 618 - 815 6e-32 Prp19 WD40 domain
Beta-prop_WDR36-Utp21_2nd PF25168 619 - 719 1.5e-06 WDR36/Utp21 second beta-propeller domain
WD40_WDHD1_1st PF24817 619 - 692 2.2e-10 WDHD1 first WD40 domain
Beta-prop_THOC3 PF25174 620 - 691 4.7e-15 THOC3 beta-propeller domain
Beta-prop_WDR3_2nd PF25172 620 - 734 1.8e-13 WDR3 second beta-propeller domain
Beta-prop_TEP1_2nd PF25047 649 - 745 3.9e-07 TEP-1 second beta-propeller
WD40 PF00400 652 - 689 1.4e-08 WD domain, G-beta repeat
WD40_CDC20-Fz PF24807 653 - 812 5e-18 CDC20/Fizzy WD40 domain
Beta-prop_THOC3 PF25174 660 - 899 7.1e-38 THOC3 beta-propeller domain
Beta-prop_WDR90_POC16_2nd PF23393 673 - 776 3.6e-06 WDR90/POC16, second beta-propeller
WD40 PF00400 695 - 733 1.2e-06 WD domain, G-beta repeat
WD40_WDHD1_1st PF24817 697 - 745 1.3e-06 WDHD1 first WD40 domain
Beta-prop_WDR3_1st PF25173 697 - 858 1.4e-19 WDR3 first beta-propeller domain
Beta-prop_EML PF23409 698 - 898 4.3e-08 Echinoderm microtubule-associated protein first beta-propeller
WDR55 PF24796 704 - 869 4.6e-08 WDR55
Beta-prop_Aladin PF25460 705 - 814 3.8e-07 Aladin seven-bladed propeller
Beta-prop_WDR36-Utp21_2nd PF25168 720 - 817 2.9e-08 WDR36/Utp21 second beta-propeller domain
Beta-prop_WDR5 PF25175 747 - 898 6.8e-24 WDR5 beta-propeller domain
WD40_WDHD1_1st PF24817 750 - 899 1.6e-13 WDHD1 first WD40 domain
Beta-prop_WDR3_2nd PF25172 752 - 899 3.2e-09 WDR3 second beta-propeller domain
WD40_Gbeta PF25391 757 - 898 3.6e-09 G protein beta WD-40 repeat protein
WD40_CDC20-Fz PF24807 759 - 898 1.2e-07 CDC20/Fizzy WD40 domain
Beta-prop_WDR36-Utp21_1st PF25171 760 - 887 5.4e-07 WDR36/Utp21 first beta-propeller
Beta-prop_EIPR1 PF23609 763 - 898 2.4e-06 EIPR1 beta-propeller
WD40 PF00400 777 - 812 6.5e-08 WD domain, G-beta repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000670)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G32551 AT4G32551
fragaria_vesca FvH4_1g04170 FvH4_1g04170 FvH4_1g04170 FvH4_1g04170 FvH4_1g04170 FvH4_1g04170 FvH4_1g04170 FvH4_1g04170 FvH4_2g29940 FvH4_2g29940 FvH4_2g29940 FvH4_2g29940 FvH4_2g29940 FvH4_2g29940 FvH4_2g29940 FvH4_2g29940
malus_domestica MD02G1040200.v1.1 MD08G1139100.v1.1 MD15G1117100.v1.1 MD15G1145100.v1.1
prunus_persica Prupe.1G468400_v2.0.a1 Prupe.1G468400_v2.0.a1 Prupe.1G468400_v2.0.a1 Prupe.1G468400_v2.0.a1 Prupe.1G468400_v2.0.a1 Prupe.1G468400_v2.0.a1 Prupe.1G468400_v2.0.a1 Prupe.1G468400_v2.0.a1 Prupe.1G468400_v2.0.a1 Prupe.1G468400_v2.0.a1 Prupe.1G468400_v2.0.a1 Prupe.1G468400_v2.0.a1 Prupe.1G468400_v2.0.a1 Prupe.1G468400_v2.0.a1 Prupe.1G468400_v2.0.a1 Prupe.1G468400_v2.0.a1 Prupe.1G468400_v2.0.a1 Prupe.7G235500_v2.0.a1 Prupe.7G235500_v2.0.a1 Prupe.7G235500_v2.0.a1 Prupe.7G235500_v2.0.a1
pyrus_communis pycom02g03350 pycom08g11750 pycom15g10550 pycom15g16250
rosa_chinensis RchiOBHm_Chr2g0089671 RchiOBHm_Chr3g0465211 RchiOBHm_Chr6g0312941
rosa_laevigata RLG00000010283 RLG00000016050
rosa_multiflora Rmu_sc0000665.1_g000023 Rmu_sc0008049.1_g000043 Rmu_sc0008049.1_g000044
rosa_roxburghii Rroxscaffold_2G00151660 Rroxscaffold_7G00156310
rosa_rugosa Rorug02G0002500 Rorug02G0002500 Rorug06G0400700 Rorug06G0400700 Rorug06G0400700 Rorug06G0400700 Rorug06G0400800.1 Rorug06G0400900.1
rosa_samantha Rh2AG047900 Rh2BG046500 Rh2CG048500 Rh2DG047900 Rh6AG517700 Rh6BG529400 Rh6CG533900 Rh6DG520500
rosa_wichuraiana Rw2G004260 Rw5G017770 Rw6G045010

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 3 cut(s) 1081, 2051, 2681
Acc65I GGTACC 1 cut(s) 1174
AccB1I GGYRCC 1 cut(s) 1174
AccB7I CCANNNNNTGG 1 cut(s) 682
AccBSI CCGCTC 1 cut(s) 940
AccI GTMKAC 3 cut(s) 812, 1105, 2335
AciI CCGC 6 cut(s) 224, 323, 467, 717, 938, 2289
AclWI GGATC 9 cut(s) 116, 533, 693, 883, 904, 1195, 1782, 2378, 2391
AcoI YGGCCR 2 cut(s) 727, 935
AcsI RAATTY 4 cut(s) 888, 1156, 1354, 2495
AcuI CTGAAG 6 cut(s) 123, 602, 720, 959, 1007, 1485
AfiI CCNNNNNNNGG 6 cut(s) 521, 682, 944, 1184, 1622, 2267
AgeI ACCGGT 1 cut(s) 2642
AjiI CACGTC 2 cut(s) 1171, 2024
AjnI CCWGG 7 cut(s) 142, 559, 805, 892, 1464, 1585, 2077
Alw26I GTCTC 5 cut(s) 9, 233, 1221, 1734, 2315
AlwI GGATC 9 cut(s) 116, 533, 693, 883, 904, 1195, 1782, 2378, 2391
AlwNI CAGNNNCTG 3 cut(s) 352, 1508, 2291
Ama87I CYCGRG 2 cut(s) 1549, 2218
AoxI GGCC 9 cut(s) 673, 727, 935, 1147, 1207, 1505, 1541, 1912, 2112
ApoI RAATTY 4 cut(s) 888, 1156, 1354, 2495
AsiGI ACCGGT 1 cut(s) 2642
Asp700I GAANNNNTTC 2 cut(s) 989, 2466
Asp718I GGTACC 1 cut(s) 1174
AspLEI GCGC 2 cut(s) 1658, 1856
AspS9I GGNCC 7 cut(s) 512, 673, 1208, 1505, 1541, 1708, 1912
AsuHPI GGTGA 6 cut(s) 674, 1071, 1178, 1182, 1674, 2405
AvaI CYCGRG 2 cut(s) 1549, 2218
AvaII GGWCC 2 cut(s) 512, 1708
AxyI CCTNAGG 1 cut(s) 870
BaeGI GKGCMC 1 cut(s) 499
BamHI GGATCC 1 cut(s) 2383
BanI GGYRCC 1 cut(s) 1174
BarI GAAGNNNNNNTAC 2 cut(s) 1663, 1695
BauI CACGAG 1 cut(s) 1221
BcgI CGANNNNNNTGC 2 cut(s) 1801, 1835
BciT130I CCWGG 7 cut(s) 144, 561, 807, 894, 1466, 1587, 2079
BciVI GTATCC 1 cut(s) 124
BclI TGATCA 1 cut(s) 1447
BcoDI GTCTC 5 cut(s) 9, 233, 1221, 1734, 2315
BfaI CTAG 4 cut(s) 195, 1905, 1970, 2667
BfmI CTRYAG 2 cut(s) 1265, 2283
BfoI RGCGCY 1 cut(s) 1659
BfuI GTATCC 1 cut(s) 124
BglI GCCNNNNNGGC 1 cut(s) 739
BlpI GCTNAGC 1 cut(s) 1062
Bme1390I CCNGG 7 cut(s) 144, 561, 807, 894, 1466, 1587, 2079
Bme18I GGWCC 2 cut(s) 512, 1708
BmeT110I CYCGRG 2 cut(s) 1549, 2218
BmgBI CACGTC 2 cut(s) 1171, 2024
BmgT120I GGNCC 7 cut(s) 512, 673, 1208, 1505, 1541, 1708, 1912
BmiI GGNNCC 3 cut(s) 1176, 1913, 2385
BmrFI CCNGG 7 cut(s) 144, 561, 807, 894, 1466, 1587, 2079
BmrI ACTGGG 1 cut(s) 25
BmsI GCATC 9 cut(s) 127, 236, 487, 628, 650, 1413, 1774, 2056, 2414
BmuI ACTGGG 1 cut(s) 25
BpmI CTGGAG 3 cut(s) 1608, 2215, 2469
Bpu1102I GCTNAGC 1 cut(s) 1062
BsaBI GATNNNNATC 2 cut(s) 690, 1773
BsaI GGTCTC 1 cut(s) 1734
BsaJI CCNNGG 2 cut(s) 576, 806
BsaWI WCCGGW 3 cut(s) 124, 817, 2642
BsaXI ACNNNNNCTCC 2 cut(s) 1567, 1597
Bsc4I CCNNNNNNNGG 6 cut(s) 521, 682, 944, 1184, 1622, 2267
Bse118I RCCGGY 2 cut(s) 725, 2642
Bse1I ACTGG 9 cut(s) 20, 842, 949, 1092, 1490, 1672, 2198, 2316, 2653
Bse21I CCTNAGG 1 cut(s) 870
Bse3DI GCAATG 1 cut(s) 189
Bse8I GATNNNNATC 2 cut(s) 690, 1773
BseBI CCWGG 7 cut(s) 144, 561, 807, 894, 1466, 1587, 2079
BseDI CCNNGG 2 cut(s) 576, 806
BseJI GATNNNNATC 2 cut(s) 690, 1773
BseLI CCNNNNNNNGG 6 cut(s) 521, 682, 944, 1184, 1622, 2267
BseMI GCAATG 1 cut(s) 189
BseMII CTCAG 7 cut(s) 20, 132, 257, 861, 1076, 2283, 2339
BseNI ACTGG 9 cut(s) 20, 842, 949, 1092, 1490, 1672, 2198, 2316, 2653
BseRI GAGGAG 1 cut(s) 1621
BseSI GKGCMC 1 cut(s) 499
BseYI CCCAGC 3 cut(s) 315, 1005, 1538
BsgI GTGCAG 1 cut(s) 69
BshFI GGCC 9 cut(s) 675, 729, 937, 1149, 1209, 1507, 1543, 1914, 2114
BshNI GGYRCC 1 cut(s) 1174
BshTI ACCGGT 1 cut(s) 2642
BsiHKCI CYCGRG 2 cut(s) 1549, 2218
BsiSI CCGG 4 cut(s) 125, 726, 818, 2643
BslFI GGGAC 1 cut(s) 2118
BslI CCNNNNNNNGG 6 cut(s) 521, 682, 944, 1184, 1622, 2267
BsmAI GTCTC 5 cut(s) 9, 233, 1221, 1734, 2315
BsmFI GGGAC 1 cut(s) 2118
BsmI GAATGC 3 cut(s) 211, 1122, 2468
BsnI GGCC 9 cut(s) 675, 729, 937, 1149, 1209, 1507, 1543, 1914, 2114
Bso31I GGTCTC 1 cut(s) 1734
BsoBI CYCGRG 2 cut(s) 1549, 2218
Bsp1286I GDGCHC 1 cut(s) 499
Bsp143I GATC 9 cut(s) 121, 538, 685, 875, 896, 1187, 1447, 1774, 2383
Bsp1720I GCTNAGC 1 cut(s) 1062
Bsp19I CCATGG 1 cut(s) 576
BspACI CCGC 6 cut(s) 224, 323, 467, 717, 938, 2289
BspANI GGCC 9 cut(s) 675, 729, 937, 1149, 1209, 1507, 1543, 1914, 2114
BspCNI CTCAG 7 cut(s) 19, 131, 256, 862, 1075, 2284, 2338
BspHI TCATGA 1 cut(s) 2470
BspLI GGNNCC 3 cut(s) 1176, 1913, 2385
BspMAI CTGCAG 1 cut(s) 2287
BspPI GGATC 9 cut(s) 116, 533, 693, 883, 904, 1195, 1782, 2378, 2391
BspT107I GGYRCC 1 cut(s) 1174
BspTNI GGTCTC 1 cut(s) 1734
BsrBI CCGCTC 1 cut(s) 940
BsrDI GCAATG 1 cut(s) 189
BsrFI RCCGGY 2 cut(s) 725, 2642
BsrI ACTGG 9 cut(s) 20, 842, 949, 1092, 1490, 1672, 2198, 2316, 2653
BssAI RCCGGY 2 cut(s) 725, 2642
BssECI CCNNGG 2 cut(s) 576, 806
BssMI GATC 9 cut(s) 121, 538, 685, 875, 896, 1187, 1447, 1774, 2383
BssSI CACGAG 1 cut(s) 1221
BssT1I CCWWGG 1 cut(s) 576
Bst2BI CACGAG 1 cut(s) 1221
Bst2UI CCWGG 7 cut(s) 144, 561, 807, 894, 1466, 1587, 2079
Bst4CI ACNGT 5 cut(s) 474, 755, 1083, 2053, 2174
Bst6I CTCTTC 4 cut(s) 59, 481, 621, 1200
BstAPI GCANNNNNTGC 3 cut(s) 352, 748, 1054
BstC8I GCNNGC 9 cut(s) 731, 1059, 1399, 1509, 1608, 1852, 1858, 2018, 2280
BstDEI CTNAG 9 cut(s) 6, 118, 243, 870, 1062, 1112, 2292, 2325, 2358
BstDSI CCRYGG 1 cut(s) 576
BstH2I RGCGCY 1 cut(s) 1659
BstHHI GCGC 2 cut(s) 1658, 1856
BstKTI GATC 9 cut(s) 124, 541, 688, 878, 899, 1190, 1450, 1777, 2386
BstMAI GTCTC 5 cut(s) 9, 233, 1221, 1734, 2315
BstMBI GATC 9 cut(s) 121, 538, 685, 875, 896, 1187, 1447, 1774, 2383
BstNI CCWGG 7 cut(s) 144, 561, 807, 894, 1466, 1587, 2079
BstNSI RCATGY 4 cut(s) 386, 867, 1426, 2020
BstSCI CCNGG 7 cut(s) 142, 559, 805, 892, 1464, 1585, 2077
BstSFI CTRYAG 2 cut(s) 1265, 2283
BstSLI GKGCMC 1 cut(s) 499
BstX2I RGATCY 2 cut(s) 121, 2383
BstYI RGATCY 2 cut(s) 121, 2383
Bsu36I CCTNAGG 1 cut(s) 870
BsuI GTATCC 1 cut(s) 124
BsuRI GGCC 9 cut(s) 675, 729, 937, 1149, 1209, 1507, 1543, 1914, 2114
BtgI CCRYGG 1 cut(s) 576
BtrI CACGTC 2 cut(s) 1171, 2024
BtsI GCAGTG 1 cut(s) 2531
BtsIMutI CAGTG 5 cut(s) 942, 1099, 1497, 2179, 2531
Cac8I GCNNGC 9 cut(s) 731, 1059, 1399, 1509, 1608, 1852, 1858, 2018, 2280
CaiI CAGNNNCTG 3 cut(s) 352, 1508, 2291
CciI TCATGA 1 cut(s) 2470
CfoI GCGC 2 cut(s) 1658, 1856
Cfr10I RCCGGY 2 cut(s) 725, 2642
Cfr13I GGNCC 7 cut(s) 512, 673, 1208, 1505, 1541, 1708, 1912
CspAI ACCGGT 1 cut(s) 2642
DdeI CTNAG 9 cut(s) 6, 118, 243, 870, 1062, 1112, 2292, 2325, 2358
DpnI GATC 9 cut(s) 123, 540, 687, 877, 898, 1189, 1449, 1776, 2385
DpnII GATC 9 cut(s) 121, 538, 685, 875, 896, 1187, 1447, 1774, 2383
DraI TTTAAA 2 cut(s) 75, 927
DrdI GACNNNNNNGTC 3 cut(s) 1081, 2051, 2681
DseDI GACNNNNNNGTC 3 cut(s) 1081, 2051, 2681
EaeI YGGCCR 2 cut(s) 727, 935
Eam1104I CTCTTC 4 cut(s) 59, 481, 621, 1200
EarI CTCTTC 4 cut(s) 59, 481, 621, 1200
Eco130I CCWWGG 1 cut(s) 576
Eco31I GGTCTC 1 cut(s) 1734
Eco47I GGWCC 2 cut(s) 512, 1708
Eco57I CTGAAG 6 cut(s) 123, 602, 720, 959, 1007, 1485
Eco81I CCTNAGG 1 cut(s) 870
Eco88I CYCGRG 2 cut(s) 1549, 2218
EcoO109I RGGNCCY 1 cut(s) 1505
EcoRI GAATTC 1 cut(s) 888
EcoRII CCWGG 7 cut(s) 142, 559, 805, 892, 1464, 1585, 2077
EcoT14I CCWWGG 1 cut(s) 576
EcoT22I ATGCAT 1 cut(s) 643
ErhI CCWWGG 1 cut(s) 576
FaqI GGGAC 1 cut(s) 2118
FbaI TGATCA 1 cut(s) 1447
FblI GTMKAC 3 cut(s) 812, 1105, 2335
FspBI CTAG 4 cut(s) 195, 1905, 1970, 2667
GlaI GCGC 2 cut(s) 1657, 1855
GsaI CCCAGC 3 cut(s) 319, 1009, 1542
GsuI CTGGAG 3 cut(s) 1608, 2215, 2469
HaeII RGCGCY 1 cut(s) 1659
HaeIII GGCC 9 cut(s) 675, 729, 937, 1149, 1209, 1507, 1543, 1914, 2114
HapII CCGG 4 cut(s) 125, 726, 818, 2643
HhaI GCGC 2 cut(s) 1658, 1856
Hin6I GCGC 2 cut(s) 1656, 1854
HinP1I GCGC 2 cut(s) 1656, 1854
HincII GTYRAC 3 cut(s) 1032, 2336, 2640
HindII GTYRAC 3 cut(s) 1032, 2336, 2640
HindIII AAGCTT 2 cut(s) 78, 90
HpaII CCGG 4 cut(s) 125, 726, 818, 2643
HphI GGTGA 6 cut(s) 674, 1071, 1178, 1182, 1674, 2405
Hpy166II GTNNAC 7 cut(s) 813, 1032, 1106, 1837, 1941, 2336, 2640
Hpy188III TCNNGA 7 cut(s) 869, 966, 1733, 2320, 2471, 2672, 2692
Hpy8I GTNNAC 7 cut(s) 813, 1032, 1106, 1837, 1941, 2336, 2640
HpyAV CCTTC 8 cut(s) 98, 744, 1135, 1554, 1572, 1918, 2397, 2450
HpyCH4III ACNGT 5 cut(s) 474, 755, 1083, 2053, 2174
HpyCH4IV ACGT 2 cut(s) 1170, 2023
HpyF3I CTNAG 9 cut(s) 6, 118, 243, 870, 1062, 1112, 2292, 2325, 2358
HpySE526I ACGT 2 cut(s) 1170, 2023
HspAI GCGC 2 cut(s) 1656, 1854
KpnI GGTACC 1 cut(s) 1178
Ksp22I TGATCA 1 cut(s) 1447
Kzo9I GATC 9 cut(s) 121, 538, 685, 875, 896, 1187, 1447, 1774, 2383
LmnI GCTCC 6 cut(s) 262, 952, 1006, 1634, 2196, 2444
LweI GCATC 9 cut(s) 127, 236, 487, 628, 650, 1413, 1774, 2056, 2414
MaeI CTAG 4 cut(s) 195, 1905, 1970, 2667
MaeII ACGT 2 cut(s) 1170, 2023
MaeIII GTNAC 5 cut(s) 1166, 1864, 1874, 2168, 2669
MalI GATC 9 cut(s) 123, 540, 687, 877, 898, 1189, 1449, 1776, 2385
MbiI CCGCTC 1 cut(s) 940
MboI GATC 9 cut(s) 121, 538, 685, 875, 896, 1187, 1447, 1774, 2383
MfeI CAATTG 1 cut(s) 800
MflI RGATCY 2 cut(s) 121, 2383
MhlI GDGCHC 1 cut(s) 499
MluCI AATT 5 cut(s) 800, 888, 1156, 1354, 2495
MlyI GAGTC 8 cut(s) 235, 766, 1135, 1570, 1757, 2317, 2414, 2590
MmeI TCCRAC 1 cut(s) 511
Mph1103I ATGCAT 1 cut(s) 643
MroXI GAANNNNTTC 2 cut(s) 989, 2466
MseI TTAA 7 cut(s) 74, 252, 605, 926, 1245, 1952, 2202
MslI CAYNNNNRTG 3 cut(s) 1235, 1409, 1782
MspA1I CMGCKG 5 cut(s) 352, 761, 1538, 2214, 2291
MspI CCGG 4 cut(s) 125, 726, 818, 2643
MspR9I CCNGG 7 cut(s) 144, 561, 807, 894, 1466, 1587, 2079
MunI CAATTG 1 cut(s) 800
Mva1269I GAATGC 3 cut(s) 211, 1122, 2468
MvaI CCWGG 7 cut(s) 144, 561, 807, 894, 1466, 1587, 2079
NcoI CCATGG 1 cut(s) 576
NdeII GATC 9 cut(s) 121, 538, 685, 875, 896, 1187, 1447, 1774, 2383
NlaIV GGNNCC 3 cut(s) 1176, 1913, 2385
NmuCI GTSAC 4 cut(s) 1166, 1874, 2168, 2669
NsiI ATGCAT 1 cut(s) 643
NspI RCATGY 4 cut(s) 386, 867, 1426, 2020
PaeI GCATGC 1 cut(s) 2020
PaeR7I CTCGAG 1 cut(s) 2218
PagI TCATGA 1 cut(s) 2470
PctI GAATGC 3 cut(s) 211, 1122, 2468
PdmI GAANNNNTTC 2 cut(s) 989, 2466
PfeI GAWTC 2 cut(s) 626, 850
PflMI CCANNNNNTGG 1 cut(s) 682
PfoI TCCNGGA 3 cut(s) 559, 892, 1585
PinAI ACCGGT 1 cut(s) 2642
PleI GAGTC 8 cut(s) 235, 766, 1134, 1570, 1756, 2317, 2414, 2590
PpsI GAGTC 8 cut(s) 235, 766, 1134, 1570, 1756, 2317, 2414, 2590
Psp6I CCWGG 7 cut(s) 142, 559, 805, 892, 1464, 1585, 2077
PspFI CCCAGC 3 cut(s) 315, 1005, 1538
PspGI CCWGG 7 cut(s) 142, 559, 805, 892, 1464, 1585, 2077
PspN4I GGNNCC 3 cut(s) 1176, 1913, 2385
PspPI GGNCC 7 cut(s) 512, 673, 1208, 1505, 1541, 1708, 1912
PspXI VCTCGAGB 1 cut(s) 2218
PstI CTGCAG 1 cut(s) 2287
PstNI CAGNNNCTG 3 cut(s) 352, 1508, 2291
PsuI RGATCY 2 cut(s) 121, 2383
PvuII CAGCTG 4 cut(s) 352, 761, 1538, 2214
RseI CAYNNNNRTG 3 cut(s) 1235, 1409, 1782
SalI GTCGAC 1 cut(s) 2334
SaqAI TTAA 7 cut(s) 74, 252, 605, 926, 1245, 1952, 2202
Sau3AI GATC 9 cut(s) 121, 538, 685, 875, 896, 1187, 1447, 1774, 2383
Sau96I GGNCC 7 cut(s) 512, 673, 1208, 1505, 1541, 1708, 1912
SchI GAGTC 8 cut(s) 235, 766, 1135, 1570, 1757, 2317, 2414, 2590
ScrFI CCNGG 7 cut(s) 144, 561, 807, 894, 1466, 1587, 2079
SduI GDGCHC 1 cut(s) 499
SfaNI GCATC 9 cut(s) 127, 236, 487, 628, 650, 1413, 1774, 2056, 2414
SfcI CTRYAG 2 cut(s) 1265, 2283
Sfr274I CTCGAG 1 cut(s) 2218
SinI GGWCC 2 cut(s) 512, 1708
SlaI CTCGAG 1 cut(s) 2218
SmiMI CAYNNNNRTG 3 cut(s) 1235, 1409, 1782
SmlI CTYRAG 1 cut(s) 2218
SmoI CTYRAG 1 cut(s) 2218
SphI GCATGC 1 cut(s) 2020
Sse9I AATT 5 cut(s) 800, 888, 1156, 1354, 2495
SsiI CCGC 6 cut(s) 224, 323, 467, 717, 938, 2289
SspI AATATT 1 cut(s) 705
SspMI CTAG 4 cut(s) 195, 1905, 1970, 2667
StyD4I CCNGG 7 cut(s) 142, 559, 805, 892, 1464, 1585, 2077
StyI CCWWGG 1 cut(s) 576
TaaI ACNGT 5 cut(s) 474, 755, 1083, 2053, 2174
TaiI ACGT 2 cut(s) 1173, 2026
TaqI TCGA 9 cut(s) 790, 1438, 1574, 1746, 1795, 2043, 2219, 2319, 2335
TaqII GACCGA 1 cut(s) 1725
TasI AATT 5 cut(s) 800, 888, 1156, 1354, 2495
TauI GCSGC 4 cut(s) 227, 719, 940, 2291
TfiI GAWTC 2 cut(s) 626, 850
Tru1I TTAA 7 cut(s) 74, 252, 605, 926, 1245, 1952, 2202
Tru9I TTAA 7 cut(s) 74, 252, 605, 926, 1245, 1952, 2202
TscAI CASTG 5 cut(s) 949, 1099, 1497, 2179, 2538
TseFI GTSAC 4 cut(s) 1166, 1874, 2168, 2669
Tsp45I GTSAC 4 cut(s) 1166, 1874, 2168, 2669
TspGWI ACGGA 4 cut(s) 1844, 2081, 2502, 2594
TspRI CASTG 5 cut(s) 949, 1099, 1497, 2179, 2538
Van91I CCANNNNNTGG 1 cut(s) 682
VpaK11BI GGWCC 2 cut(s) 512, 1708
XapI RAATTY 4 cut(s) 888, 1156, 1354, 2495
XceI RCATGY 4 cut(s) 386, 867, 1426, 2020
XhoI CTCGAG 1 cut(s) 2218
XmiI GTMKAC 3 cut(s) 812, 1105, 2335
XmnI GAANNNNTTC 2 cut(s) 989, 2466
XspI CTAG 4 cut(s) 195, 1905, 1970, 2667
Zsp2I ATGCAT 1 cut(s) 643
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.