AT5G46540

ABC transporter B family member

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0001944
Physical Location & Seq
Reverse (-)
18877090 .. 18882462
5373 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G46540.1

Sequence Viewer

Length: 3747 bp
ATGGCGGAGAAAGCGAAGAAGAAGAAAAATGGAGGAGGAGGAAATCAGAGAATAGCGTTTTACAAGCTTTTCACGTTTGCAGATCGGTACGACATCGTTTTAATGGTCATCGGGACCCTCTCCGCCATGGCCAACGGCTTAACTCAGCCGTTTATGTCAATACTCATGGGGCAGCTCATTAACGTTTTTGGTTTCTCTGATCATGATCACGTGTTTAAAGAAGTCTCCAAGGTTGCTGTGAAGTTTCTTTATCTTGCAGCCTACGCCGGTGTTGTGTCCTTCCTGCAGGTCTCATGCTGGATGGTAACTGGGGAGCGTCAATCCACTAGAATCCGCCGTCTATACCTAAAAACAATTTTAAGACAAGACATTGGATTTTTCGATACTGAGACAAATACCGGTGAAGTCATCGGAAGAATGTCAGGAGACACCATTCTTATTCAAGATTCCATGGGAGAAAAGGTTGGGAAATTCACACAGCTCGTTTCGTCATTCGTTGGTGGATTCACGGTTGCTTTTATAGTTGGAATGAAACTCACATTGGCTCTTCTTCCGTGCGTTCCTCTGATCGTGGGCACAGGTGGAGCAATGACCTATATCATGTCAAAAAAGGCTCAACGTGTTCAACTGGCTTACACAGAAGCTGGAAACGTAGTACAACAAGCCGTTGGGTCAATTAGAACAGTTGTGGCTTTCACTGGGGAGAAACAATCAATGGGAAAATATGAGAAGAAGCTTGAGATAGCCTACAAATCTATGGTTAAACAAGGGCTATACTCCGGTCTAGGCATTGGGATAATGATGGTTGTGGTATATTGTACTTATGGTTTTGCGATTTGGTACGGTGCTAGGCAGATAATAGAGAAAGGATATACTGGAGGTCAAGTCATGAACGTGATTACGTCAATCTTGACTGGAGGAATGGCGTTGGGGCAGACATTGCCTTCTCTTAACTCTTTTGCGGCCGGAACAGCTGCAGCTTACAAAATGTTTGAGACTATCAAAAGAAAACCAAAGATCGATGCTTATGACATGAGTGGTGAGGTTTTAGAAGAAATTAAAGGTGACATTGAGCTAAGAGACGTGTACTTTCGATATCCGGCTAGACCTGACGTGCAGATATTTGTTGGATTTTCGTTGACTGTACCGAATGGCATGACCGTGGCTTTGGTGGGACAGAGCGGTAGCGGAAAATCAACTGTGATCAGTTTAATTGAGAGGTTCTACGATCCGGAATCAGGAGAAGTTTTAATAGATGGAATTGACTTAAAGAAATTTCAAGTGAAATGGATCAGAAGTAAGATTGGTCTCGTAAGCCAAGAACCTATACTATTTGCTACCACAATTAGGGAGAACATTGTTTACGGCAAAAAAGATGCGTCCGACCAAGAAATTAGAACCGCACTTAAGCTCGCCAACGCTAGTAACTTCATCGACAAACTTCCGCAGGGTTTGGAAACGATGGTAGGAGAGCATGGGACGCAATTATCCGGTGGGCAAAAGCAGAGGATTGCGATTGCGAGGGCGATTTTGAAAAACCCAAAAATATTGCTTCTTGATGAAGCTACAAGCGCTTTGGACGCAGAATCTGAACGCATTGTTCAAGATGCTCTTGTAAAGCTAATGTTGAGCAGGACCACTGTAGTGGTGGCTCACCGACTTACCACCATTAGAACCGCTGATATGATCGCTGTTGTGCAGCAGGGAAAAGTCATTGAGAAAGGAACTCATGATGAGATGATTAAAGATCCCGAAGGAACATATTCACAGCTAGTTCGTCTACAAGAAGGATCTAAGAAAGAAGAAGCAATAGACAAGGAACCAGAAAAGTGTGAAATGAGTTTGGAAATCGAAAGTTCGGATAGCCAAAATGGAATCCATTCCGGCACTCTCACATCACCATCTGGTTTACCGGGAGTGATTAGCTTGGACCAGACGGAAGAGTTCCACGAGAATATATCTTCCACCAAAACTCAAACGGTTAAGAAAGGCAAGGAAGTATCATTAAGAAGGCTTGCTCACCTTAACAAACCGGAGATTTCGGTTTTGTTGCTCGGTTCATTGGCTGCAGTGATTCACGGTATTGTGTTCCCTGTTCAAGGTTTGCTTTTGTCTCGCACAATCAGGATATTCTTTGAGCCTTCAAATAAACTAAAGAATGATTCTCTTTTCTGGGCTCTCATTTTTGTCGCCCTTGGCTTAACCGACCTGATTGTGATTCCGTTACAAAACTACTTATTCGCTATCGCTGGAGCAAAACTAATCAAACGTATCAGATCGTTGTCGTTTGATAGAGTCTTGCACCAAGATATAAGTTGGTTCGACGATACTAAAAATTCTAGCGGTGTGATTGGAGCGAGGTTGTCCACGGATGCTTCTACAGTGAAGAGTATAGTAGGAGATGTGTTAGGGTTGATCATGCAAAATATGGCGACTATAATTGGAGCATTTATTATAGCGTTTACGGCTAATTGGTTATTAGCTTTAATGGCCCTTTTGGTGGCACCAGTAATGTTTTTTCAAGGATACTACCAGATCAAGTTTATAACGGGTTTTGGAGCAAAAGCAAGAGGAAAATATGAAGAGGCTAGCCAGGTTGCGAGTGACGCGGTGAGCAGCATAAGAACAGTTGCATCGTTCTGCGCAGAGGATAAAGTGATGGATTTATACCAAGAGAAATGTGACGAACCAAAGCAACAAGGCTTCAAATTAGGACTGGTGAGTGGTTTGTGCTATGGTGGCTCATACTTGGCTCTCTATGTCATCGAGTCCGTATGCTTTCTCGGCGGTTCCTGGTTGATTCAGAACAGGAGGGCAACGTTCGGAGAATTCTTTCAGGTTTTCTTCGCGTTAACTTTGACAGCCGTAGGAGTGACTCAAACTAGTACTATGGCACCTGACATAAACAAAGCAAAAGACTCTGCTGCTTCGATATTCGATATCCTCGATAGTAAACCGAAAATCGATTCGAGTTCCGAGAAAGGGACAATATTACCAATCGTCCACGGCGATATCGAGTTGCAACATGTTAGTTTCCGGTATCCTATGCGACCGGATATACAGATTTTTAGTGACTTGTGCTTAACTATCTCATCTGGACAGACCGTTGCATTGGTTGGAGAAAGCGGGAGCGGTAAATCGACGGTGATAAGCTTACTAGAGAGATTCTACGATCCGGATTCAGGCAAGATTCTTTTAGATCAAGTTGAAATTCAATCGTTAAAATTGAGTTGGTTAAGGGAACAAATGGGTTTGGTTAGTCAAGAACCGGTTCTGTTCAATGAGACAATAGGATCAAACATAGCATATGGTAAAATCGGAGGAGCCACAGAGGAAGAGATTATAACCGCGGCAAAAGCGGCTAATGTGCATAACTTCATCAGCTCATTGCCTCAAGGCTACGAAACGTCCGTTGGTGAACGTGGAGTACAATTATCCGGTGGTCAAAAACAGAGGATCGCGATCGCTCGGGCTATTCTCAAGGATCCTAAAATTTTGTTGCTTGATGAAGCGACAAGCGCGTTGGATGCAGAGTCTGAGCGGGTGGTGCAAGATGCGCTTGATCAGGTGATGGTGAACAGGACCACGGTTGTGGTGGCTCACCTGCTCACTACCATTAAGGACGCTGATATGATCGCGGTTGTGAAGAACGGTGTGATCGCGGAGAGTGGGAGACATGAAACGTTAATGGAGATTTCAGGTGGTGCTTATGCATCGCTTGTAGCTTTTAATATGAGTGCAAACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
ko02010 ko04976 ko05206 ko05226 map02010 map04976 map05206 map05226
Pfam Domains
Protein Families
ABC_membrane ABC_tran

Protein Analysis

1248

Amino Acids

136.11

Weight (kDa)

7.22

Isoelectric Point (pI)

34.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ABC_membrane PF00664 35 - 306 3.7e-62 ABC transporter transmembrane region
ABC_tran PF00005 378 - 524 3.2e-34 ABC transporter
ABC_membrane PF00664 684 - 955 1.5e-56 ABC transporter transmembrane region
ABC_tran PF00005 1025 - 1173 2.5e-33 ABC transporter
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 2546, 3344
AarI CACCTGC 1 cut(s) 3612
Acc16I TGCGCA 1 cut(s) 2644
Acc36I ACCTGC 2 cut(s) 277, 3612
AccB1I GGYRCC 2 cut(s) 2503, 2893
AccBSI CCGCTC 3 cut(s) 1182, 3132, 3541
AccI GTMKAC 1 cut(s) 1780
AccII CGCG 7 cut(s) 2609, 2849, 3350, 3461, 3521, 3638, 3662
AccIII TCCGGA 2 cut(s) 1231, 3175
AclI AACGTT 3 cut(s) 183, 2819, 3683
AclWI GGATC 9 cut(s) 1223, 1298, 1742, 1798, 3167, 3301, 3464, 3479, 3492
AcoI YGGCCR 2 cut(s) 129, 963
AcsI RAATTY 6 cut(s) 470, 1274, 2335, 2828, 3210, 3492
AcvI CACGTG 1 cut(s) 211
AfaI GTAC 8 cut(s) 89, 657, 820, 842, 1088, 1146, 2887, 3429
AfeI AGCGCT 1 cut(s) 1573
AfiI CCNNNNNNNGG 6 cut(s) 1238, 1347, 2099, 2500, 2982, 3182
AflII CTTAAG 1 cut(s) 1406
AflIII ACRYGT 4 cut(s) 210, 619, 1083, 3025
AgeI ACCGGT 2 cut(s) 398, 3268
AhlI ACTAGT 1 cut(s) 2882
AjiI CACGTC 2 cut(s) 1084, 1114
AjnI CCWGG 2 cut(s) 2592, 2792
AjuI GAANNNNNNNTTGG 4 cut(s) 524, 556, 3396, 3428
AleI CACNNNNGTG 2 cut(s) 1643, 3590
AlwI GGATC 9 cut(s) 1223, 1298, 1742, 1798, 3167, 3301, 3464, 3479, 3492
AlwNI CAGNNNCTG 3 cut(s) 644, 1589, 3536
Ama87I CYCGRG 1 cut(s) 3468
Aor13HI TCCGGA 2 cut(s) 1231, 3175
Aor51HI AGCGCT 1 cut(s) 1573
AoxI GGCC 3 cut(s) 129, 963, 2490
ApeKI GCWGC 8 cut(s) 172, 257, 974, 977, 1699, 2066, 2616, 2924
ApoI RAATTY 6 cut(s) 470, 1274, 2335, 2828, 3210, 3492
ArsI GACNNNNNNTTYG 2 cut(s) 1150, 1182
AsiGI ACCGGT 2 cut(s) 398, 3268
AsiSI GCGATCGC 1 cut(s) 3465
Asp700I GAANNNNTTC 4 cut(s) 1762, 1879, 2832, 3270
AspLEI GCGC 4 cut(s) 1574, 2645, 3521, 3559
AspS9I GGNCC 5 cut(s) 114, 1635, 1930, 2491, 3582
AsuC2I CCSGG 1 cut(s) 1914
AsuNHI GCTAGC 1 cut(s) 2588
AvaI CYCGRG 1 cut(s) 3468
AvaII GGWCC 4 cut(s) 114, 1635, 1930, 3582
BaeGI GKGCMC 1 cut(s) 578
BalI TGGCCA 1 cut(s) 131
BamHI GGATCC 1 cut(s) 3484
BanI GGYRCC 2 cut(s) 2503, 2893
BanII GRGCYC 1 cut(s) 2179
BauI CACGAG 1 cut(s) 1949
BbrPI CACGTG 1 cut(s) 211
BbvI GCAGC 8 cut(s) 184, 269, 961, 989, 1711, 2053, 2628, 2911
BccI CCATC 7 cut(s) 295, 796, 1250, 1456, 1909, 2653, 3565
BceAI ACGGC 8 cut(s) 133, 151, 321, 650, 1381, 2481, 2849, 3022
BciT130I CCWGG 2 cut(s) 2594, 2794
BciVI GTATCC 2 cut(s) 2519, 3051
BclI TGATCA 5 cut(s) 199, 205, 1203, 2415, 3562
BcnI CCSGG 1 cut(s) 1914
BcuI ACTAGT 1 cut(s) 2882
BfmI CTRYAG 5 cut(s) 284, 975, 1641, 2067, 2379
BfoI RGCGCY 1 cut(s) 1575
BfrI CTTAAG 1 cut(s) 1406
BfuAI ACCTGC 2 cut(s) 277, 3612
BfuI GTATCC 2 cut(s) 2519, 3051
BmcAI AGTACT 1 cut(s) 2887
Bme1390I CCNGG 3 cut(s) 1914, 2594, 2794
Bme18I GGWCC 4 cut(s) 114, 1635, 1930, 3582
BmeT110I CYCGRG 1 cut(s) 3468
BmgBI CACGTC 2 cut(s) 1084, 1114
BmgT120I GGNCC 5 cut(s) 114, 1635, 1930, 2491, 3582
BmiI GGNNCC 8 cut(s) 115, 116, 1821, 2505, 2791, 2895, 3325, 3486
BmrFI CCNGG 3 cut(s) 1914, 2594, 2794
BmrI ACTGGG 2 cut(s) 318, 708
BmsI GCATC 8 cut(s) 1012, 1366, 1597, 2362, 2642, 3517, 3544, 3722
BmtI GCTAGC 1 cut(s) 2592
BmuI ACTGGG 2 cut(s) 318, 708
BpmI CTGGAG 3 cut(s) 897, 936, 2271
BpuEI CTTGAG 3 cut(s) 758, 3378, 3464
BpuMI CCSGG 1 cut(s) 1914
Bsa29I ATCGAT 2 cut(s) 1020, 2964
BsaAI YACGTR 1 cut(s) 211
BsaBI GATNNNNATC 2 cut(s) 204, 3461
BsaI GGTCTC 2 cut(s) 295, 1313
BsaJI CCNNGG 9 cut(s) 126, 228, 450, 1161, 2194, 2367, 3004, 3348, 3585
BsaXI ACNNNNNCTCC 4 cut(s) 447, 477, 870, 900
Bsc4I CCNNNNNNNGG 6 cut(s) 1238, 1347, 2099, 2500, 2982, 3182
Bse118I RCCGGY 3 cut(s) 266, 398, 3268
Bse1I ACTGG 7 cut(s) 313, 633, 703, 880, 919, 2507, 2721
Bse3DI GCAATG 3 cut(s) 594, 938, 3386
Bse8I GATNNNNATC 2 cut(s) 204, 3461
BseAI TCCGGA 2 cut(s) 1231, 3175
BseBI CCWGG 2 cut(s) 2594, 2794
BseCI ATCGAT 2 cut(s) 1020, 2964
BseDI CCNNGG 9 cut(s) 126, 228, 450, 1161, 2194, 2367, 3004, 3348, 3585
BseGI GGATG 3 cut(s) 306, 2377, 3532
BseJI GATNNNNATC 2 cut(s) 204, 3461
BseLI CCNNNNNNNGG 6 cut(s) 1238, 1347, 2099, 2500, 2982, 3182
BseMI GCAATG 3 cut(s) 594, 938, 3386
BseMII CTCAG 3 cut(s) 158, 378, 3528
BseNI ACTGG 7 cut(s) 313, 633, 703, 880, 919, 2507, 2721
BseRI GAGGAG 3 cut(s) 48, 51, 3336
BseSI GKGCMC 1 cut(s) 578
BseX3I CGGCCG 1 cut(s) 963
BseXI GCAGC 8 cut(s) 184, 269, 961, 989, 1711, 2053, 2628, 2911
BsgI GTGCAG 2 cut(s) 1136, 1718
Bsh1236I CGCG 7 cut(s) 2609, 2849, 3350, 3461, 3521, 3638, 3662
Bsh1285I CGRYCG 3 cut(s) 966, 3053, 3465
BshFI GGCC 3 cut(s) 131, 965, 2492
BshNI GGYRCC 2 cut(s) 2503, 2893
BshTI ACCGGT 2 cut(s) 398, 3268
BshVI ATCGAT 2 cut(s) 1020, 2964
BsiEI CGRYCG 3 cut(s) 966, 3053, 3465
BsiHKCI CYCGRG 1 cut(s) 3468
BslFI GGGAC 4 cut(s) 127, 1188, 1492, 2998
BslI CCNNNNNNNGG 6 cut(s) 1238, 1347, 2099, 2500, 2982, 3182
BsmBI CGTCTC 1 cut(s) 1074
BsmFI GGGAC 4 cut(s) 127, 1188, 1492, 2998
BsnI GGCC 3 cut(s) 131, 965, 2492
Bso31I GGTCTC 2 cut(s) 295, 1313
BsoBI CYCGRG 1 cut(s) 3468
Bsp1286I GDGCHC 2 cut(s) 578, 2179
Bsp13I TCCGGA 2 cut(s) 1231, 3175
Bsp19I CCATGG 2 cut(s) 126, 450
Bsp68I TCGCGA 1 cut(s) 3461
BspANI GGCC 3 cut(s) 131, 965, 2492
BspCNI CTCAG 3 cut(s) 157, 379, 3529
BspDI ATCGAT 2 cut(s) 1020, 2964
BspEI TCCGGA 2 cut(s) 1231, 3175
BspFNI CGCG 7 cut(s) 2609, 2849, 3350, 3461, 3521, 3638, 3662
BspHI TCATGA 3 cut(s) 202, 888, 1729
BspLI GGNNCC 8 cut(s) 115, 116, 1821, 2505, 2791, 2895, 3325, 3486
BspMAI CTGCAG 3 cut(s) 288, 979, 2071
BspMI ACCTGC 2 cut(s) 277, 3612
BspOI GCTAGC 1 cut(s) 2592
BspPI GGATC 9 cut(s) 1223, 1298, 1742, 1798, 3167, 3301, 3464, 3479, 3492
BspQI GCTCTTC 1 cut(s) 552
BspT107I GGYRCC 2 cut(s) 2503, 2893
BspTI CTTAAG 1 cut(s) 1406
BspTNI GGTCTC 2 cut(s) 295, 1313
BsrBI CCGCTC 3 cut(s) 1182, 3132, 3541
BsrDI GCAATG 3 cut(s) 594, 938, 3386
BsrFI RCCGGY 3 cut(s) 266, 398, 3268
BsrI ACTGG 7 cut(s) 313, 633, 703, 880, 919, 2507, 2721
BssAI RCCGGY 3 cut(s) 266, 398, 3268
BssECI CCNNGG 9 cut(s) 126, 228, 450, 1161, 2194, 2367, 3004, 3348, 3585
BssSI CACGAG 1 cut(s) 1949
BssT1I CCWWGG 4 cut(s) 126, 228, 450, 2194
Bst2BI CACGAG 1 cut(s) 1949
Bst2UI CCWGG 2 cut(s) 2594, 2794
Bst6I CTCTTC 5 cut(s) 552, 1935, 2381, 2577, 3330
BstAFI CTTAAG 1 cut(s) 1406
BstAPI GCANNNNNTGC 1 cut(s) 940
BstBAI YACGTR 1 cut(s) 211
BstC8I GCNNGC 3 cut(s) 1413, 2016, 2590
BstDEI CTNAG 5 cut(s) 144, 387, 1076, 1794, 3537
BstDSI CCRYGG 7 cut(s) 126, 450, 1161, 2367, 3004, 3348, 3585
BstENI CCTNNNNNAGG 1 cut(s) 2097
BstF5I GGATG 3 cut(s) 306, 2377, 3532
BstFNI CGCG 7 cut(s) 2609, 2849, 3350, 3461, 3521, 3638, 3662
BstH2I RGCGCY 1 cut(s) 1575
BstHHI GCGC 4 cut(s) 1574, 2645, 3521, 3559
BstMCI CGRYCG 3 cut(s) 966, 3053, 3465
BstNI CCWGG 2 cut(s) 2594, 2794
BstNSI RCATGY 1 cut(s) 3029
BstSCI CCNGG 3 cut(s) 1912, 2592, 2792
BstSFI CTRYAG 5 cut(s) 284, 975, 1641, 2067, 2379
BstSLI GKGCMC 1 cut(s) 578
BstUI CGCG 7 cut(s) 2609, 2849, 3350, 3461, 3521, 3638, 3662
BstV1I GCAGC 8 cut(s) 184, 269, 961, 989, 1711, 2053, 2628, 2911
BstX2I RGATCY 3 cut(s) 1747, 1790, 3484
BstXI CCANNNNNNTGG 2 cut(s) 1645, 3592
BstYI RGATCY 3 cut(s) 1747, 1790, 3484
BstZI CGGCCG 1 cut(s) 963
Bsu15I ATCGAT 2 cut(s) 1020, 2964
BsuI GTATCC 2 cut(s) 2519, 3051
BsuRI GGCC 3 cut(s) 131, 965, 2492
BsuTUI ATCGAT 2 cut(s) 1020, 2964
BtgI CCRYGG 7 cut(s) 126, 450, 1161, 2367, 3004, 3348, 3585
BtgZI GCGATG 1 cut(s) 3699
BtrI CACGTC 2 cut(s) 1084, 1114
BtsCI GGATG 3 cut(s) 306, 2377, 3532
BtsI GCAGTG 1 cut(s) 2076
BtsIMutI CAGTG 4 cut(s) 696, 1638, 2076, 2388
BtuMI TCGCGA 1 cut(s) 3461
BveI ACCTGC 2 cut(s) 277, 3612
Cac8I GCNNGC 3 cut(s) 1413, 2016, 2590
CaiI CAGNNNCTG 3 cut(s) 644, 1589, 3536
CciI TCATGA 3 cut(s) 202, 888, 1729
CfoI GCGC 4 cut(s) 1574, 2645, 3521, 3559
Cfr10I RCCGGY 3 cut(s) 266, 398, 3268
Cfr13I GGNCC 5 cut(s) 114, 1635, 1930, 2491, 3582
Cfr42I CCGCGG 1 cut(s) 3351
ClaI ATCGAT 2 cut(s) 1020, 2964
CseI GACGC 6 cut(s) 305, 1368, 1489, 1589, 2615, 3632
Csp6I GTAC 8 cut(s) 88, 656, 819, 841, 1087, 1145, 2886, 3428
CspAI ACCGGT 2 cut(s) 398, 3268
CviQI GTAC 8 cut(s) 88, 656, 819, 841, 1087, 1145, 2886, 3428
DdeI CTNAG 5 cut(s) 144, 387, 1076, 1794, 3537
DraI TTTAAA 1 cut(s) 217
EaeI YGGCCR 2 cut(s) 129, 963
EagI CGGCCG 1 cut(s) 963
Eam1104I CTCTTC 5 cut(s) 552, 1935, 2381, 2577, 3330
EarI CTCTTC 5 cut(s) 552, 1935, 2381, 2577, 3330
EciI GGCGGA 3 cut(s) 20, 112, 323
EclXI CGGCCG 1 cut(s) 963
Eco130I CCWWGG 4 cut(s) 126, 228, 450, 2194
Eco24I GRGCYC 1 cut(s) 2179
Eco31I GGTCTC 2 cut(s) 295, 1313
Eco32I GATATC 3 cut(s) 1097, 2941, 3013
Eco47I GGWCC 4 cut(s) 114, 1635, 1930, 3582
Eco47III AGCGCT 1 cut(s) 1573
Eco52I CGGCCG 1 cut(s) 963
Eco72I CACGTG 1 cut(s) 211
Eco88I CYCGRG 1 cut(s) 3468
EcoNI CCTNNNNNAGG 1 cut(s) 2097
EcoO109I RGGNCCY 1 cut(s) 114
EcoRI GAATTC 1 cut(s) 2828
EcoRII CCWGG 2 cut(s) 2592, 2792
EcoRV GATATC 3 cut(s) 1097, 2941, 3013
EcoT14I CCWWGG 4 cut(s) 126, 228, 450, 2194
EcoT22I ATGCAT 1 cut(s) 3715
EcoT38I GRGCYC 1 cut(s) 2179
ErhI CCWWGG 4 cut(s) 126, 228, 450, 2194
Esp3I CGTCTC 1 cut(s) 1074
FalI AAGNNNNNCTT 6 cut(s) 1596, 1628, 2091, 2123, 3543, 3575
FaqI GGGAC 4 cut(s) 127, 1188, 1492, 2998
FauI CCCGC 2 cut(s) 3119, 3534
FauNDI CATATG 1 cut(s) 3307
FbaI TGATCA 5 cut(s) 199, 205, 1203, 2415, 3562
FblI GTMKAC 1 cut(s) 1780
FokI GGATG 3 cut(s) 313, 2384, 3539
FriOI GRGCYC 1 cut(s) 2179
FspI TGCGCA 1 cut(s) 2644
GlaI GCGC 4 cut(s) 1573, 2644, 3520, 3558
GsuI CTGGAG 3 cut(s) 897, 936, 2271
HaeII RGCGCY 1 cut(s) 1575
HaeIII GGCC 3 cut(s) 131, 965, 2492
HgaI GACGC 6 cut(s) 305, 1368, 1489, 1589, 2615, 3632
HhaI GCGC 4 cut(s) 1574, 2645, 3521, 3559
Hin6I GCGC 4 cut(s) 1572, 2643, 3519, 3557
HinP1I GCGC 4 cut(s) 1572, 2643, 3519, 3557
HincII GTYRAC 2 cut(s) 1140, 2853
HindII GTYRAC 2 cut(s) 1140, 2853
HindIII AAGCTT 3 cut(s) 65, 734, 3151
HpaI GTTAAC 1 cut(s) 2853
Hpy99I CGWCG 2 cut(s) 2327, 3145
HpyAV CCTTC 6 cut(s) 289, 954, 1748, 1781, 2004, 2151
HpyF3I CTNAG 5 cut(s) 144, 387, 1076, 1794, 3537
HspAI GCGC 4 cut(s) 1572, 2643, 3519, 3557
KflI GGGWCCC 1 cut(s) 114
Kpn2I TCCGGA 2 cut(s) 1231, 3175
Ksp22I TGATCA 5 cut(s) 199, 205, 1203, 2415, 3562
KspAI GTTAAC 1 cut(s) 2853
KspI CCGCGG 1 cut(s) 3351
LguI GCTCTTC 1 cut(s) 552
LmnI GCTCC 8 cut(s) 313, 584, 2252, 2354, 2444, 2558, 3129, 3323
Lsp1109I GCAGC 8 cut(s) 184, 269, 961, 989, 1711, 2053, 2628, 2911
LweI GCATC 8 cut(s) 1012, 1366, 1597, 2362, 2642, 3517, 3544, 3722
MaeIII GTNAC 8 cut(s) 304, 1064, 1424, 2223, 2603, 2681, 2872, 3071
MbiI CCGCTC 3 cut(s) 1182, 3132, 3541
MflI RGATCY 3 cut(s) 1747, 1790, 3484
MhlI GDGCHC 2 cut(s) 578, 2179
MlsI TGGCCA 1 cut(s) 131
MluNI TGGCCA 1 cut(s) 131
MlyI GAGTC 5 cut(s) 2304, 2777, 2869, 2912, 3542
MmeI TCCRAC 5 cut(s) 505, 1108, 1407, 3097, 3504
Mox20I TGGCCA 1 cut(s) 131
Mph1103I ATGCAT 1 cut(s) 3715
MroI TCCGGA 2 cut(s) 1231, 3175
MroXI GAANNNNTTC 4 cut(s) 1762, 1879, 2832, 3270
MscI TGGCCA 1 cut(s) 131
MslI CAYNNNNRTG 4 cut(s) 893, 1160, 1643, 3590
Msp20I TGGCCA 1 cut(s) 131
MspA1I CMGCKG 3 cut(s) 974, 1679, 3350
MspCI CTTAAG 1 cut(s) 1406
MspR9I CCNGG 3 cut(s) 1914, 2594, 2794
MvaI CCWGG 2 cut(s) 2594, 2794
MvnI CGCG 7 cut(s) 2609, 2849, 3350, 3461, 3521, 3638, 3662
NciI CCSGG 1 cut(s) 1914
NcoI CCATGG 2 cut(s) 126, 450
NdeI CATATG 1 cut(s) 3307
NheI GCTAGC 1 cut(s) 2588
NlaIV GGNNCC 8 cut(s) 115, 116, 1821, 2505, 2791, 2895, 3325, 3486
NmeAIII GCCGAG 1 cut(s) 2763
NmuCI GTSAC 5 cut(s) 1064, 2603, 2681, 2872, 3071
NruI TCGCGA 1 cut(s) 3461
NsbI TGCGCA 1 cut(s) 2644
NsiI ATGCAT 1 cut(s) 3715
NspI RCATGY 1 cut(s) 3029
OliI CACNNNNGTG 2 cut(s) 1643, 3590
PagI TCATGA 3 cut(s) 202, 888, 1729
PaqCI CACCTGC 1 cut(s) 3612
PciI ACATGT 1 cut(s) 3025
PciSI GCTCTTC 1 cut(s) 552
PcsI WCGNNNNNNNCGW 4 cut(s) 2943, 3006, 3012, 3408
PdmI GAANNNNTTC 4 cut(s) 1762, 1879, 2832, 3270
PinAI ACCGGT 2 cut(s) 398, 3268
Ple19I CGATCG 1 cut(s) 3465
PleI GAGTC 5 cut(s) 2303, 2776, 2869, 2912, 3541
PmaCI CACGTG 1 cut(s) 211
PmlI CACGTG 1 cut(s) 211
PpsI GAGTC 5 cut(s) 2303, 2776, 2869, 2912, 3541
Ppu21I YACGTR 1 cut(s) 211
PpuMI RGGWCCY 1 cut(s) 114
PscI ACATGT 1 cut(s) 3025
PsiI TTATAA 2 cut(s) 2546, 3344
Psp1406I AACGTT 3 cut(s) 183, 2819, 3683
Psp5II RGGWCCY 1 cut(s) 114
Psp6I CCWGG 2 cut(s) 2592, 2792
PspCI CACGTG 1 cut(s) 211
PspGI CCWGG 2 cut(s) 2592, 2792
PspN4I GGNNCC 8 cut(s) 115, 116, 1821, 2505, 2791, 2895, 3325, 3486
PspPI GGNCC 5 cut(s) 114, 1635, 1930, 2491, 3582
PspPPI RGGWCCY 1 cut(s) 114
PsrI GAACNNNNNNTAC 4 cut(s) 1346, 1378, 3411, 3443
PstI CTGCAG 3 cut(s) 288, 979, 2071
PstNI CAGNNNCTG 3 cut(s) 644, 1589, 3536
PsuI RGATCY 3 cut(s) 1747, 1790, 3484
PvuI CGATCG 1 cut(s) 3465
PvuII CAGCTG 1 cut(s) 974
RgaI GCGATCGC 1 cut(s) 3465
RruI TCGCGA 1 cut(s) 3461
RsaI GTAC 8 cut(s) 89, 657, 820, 842, 1088, 1146, 2887, 3429
RsaNI GTAC 8 cut(s) 88, 656, 819, 841, 1087, 1145, 2886, 3428
RseI CAYNNNNRTG 4 cut(s) 893, 1160, 1643, 3590
SacII CCGCGG 1 cut(s) 3351
SapI GCTCTTC 1 cut(s) 552
Sau96I GGNCC 5 cut(s) 114, 1635, 1930, 2491, 3582
SbfI CCTGCAGG 1 cut(s) 288
ScaI AGTACT 1 cut(s) 2887
SchI GAGTC 5 cut(s) 2304, 2777, 2869, 2912, 3542
ScrFI CCNGG 3 cut(s) 1914, 2594, 2794
SdaI CCTGCAGG 1 cut(s) 288
SduI GDGCHC 2 cut(s) 578, 2179
SfaAI GCGATCGC 1 cut(s) 3465
SfaNI GCATC 8 cut(s) 1012, 1366, 1597, 2362, 2642, 3517, 3544, 3722
SfcI CTRYAG 5 cut(s) 284, 975, 1641, 2067, 2379
Sfr303I CCGCGG 1 cut(s) 3351
SgfI GCGATCGC 1 cut(s) 3465
SgrAI CRCCGGYG 1 cut(s) 266
SgrBI CCGCGG 1 cut(s) 3351
SinI GGWCC 4 cut(s) 114, 1635, 1930, 3582
SmiMI CAYNNNNRTG 4 cut(s) 893, 1160, 1643, 3590
SmlI CTYRAG 4 cut(s) 737, 1406, 3393, 3479
SmoI CTYRAG 4 cut(s) 737, 1406, 3393, 3479
SpeI ACTAGT 1 cut(s) 2882
Sse8387I CCTGCAGG 1 cut(s) 288
SspI AATATT 2 cut(s) 1548, 2991
StyD4I CCNGG 3 cut(s) 1912, 2592, 2792
StyI CCWWGG 4 cut(s) 126, 228, 450, 2194
TatI WGTACW 5 cut(s) 655, 818, 1086, 2885, 3427
TauI GCSGC 3 cut(s) 965, 3353, 3362
TscAI CASTG 4 cut(s) 703, 1645, 2076, 2388
TseFI GTSAC 5 cut(s) 1064, 2603, 2681, 2872, 3071
TseI GCWGC 8 cut(s) 172, 257, 974, 977, 1699, 2066, 2616, 2924
Tsp45I GTSAC 5 cut(s) 1064, 2603, 2681, 2872, 3071
TspDTI ATGAA 9 cut(s) 545, 905, 1420, 1575, 2049, 2595, 3367, 3522, 3693
TspGWI ACGGA 6 cut(s) 543, 1952, 2211, 2384, 2761, 3400
TspRI CASTG 4 cut(s) 703, 1645, 2076, 2388
Vha464I CTTAAG 1 cut(s) 1406
VpaK11BI GGWCC 4 cut(s) 114, 1635, 1930, 3582
XagI CCTNNNNNAGG 1 cut(s) 2097
XapI RAATTY 6 cut(s) 470, 1274, 2335, 2828, 3210, 3492
XceI RCATGY 1 cut(s) 3029
XcmI CCANNNNNNNNNTGG 2 cut(s) 1645, 3592
XmiI GTMKAC 1 cut(s) 1780
XmnI GAANNNNTTC 4 cut(s) 1762, 1879, 2832, 3270
ZrmI AGTACT 1 cut(s) 2887
Zsp2I ATGCAT 1 cut(s) 3715
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.