MD05G1292400.v1.1

ABC transporter B family member

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0001944
Physical Location & Seq
Reverse (-)
42368248 .. 42373839
5592 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1292400.v1.1.491

Sequence Viewer

Length: 3273 bp
ATGATCGTTGCAACGATATGTGCCGTGGGGAATGGACTGTCGCCGCCGGTCATGACTCTTGTGTTTGGCAATCTTATCAACACCTTTGGTTCCACCGATCCTGGTCATATCGTTCCCATGGTCTCTAAGGTGTCTCTACAATTTGTGTACTTGGCCATTGGCACTGGAGCTGCGGCACTTCTACAGGTGGCATGTTGGCTGGTGACAGGAGAAAGGCAGGCTGCTAGAATTAGAGGCAAGTACATGAAGGCAATCCTAAGGCAGGACATTGCCTTCTTTGACACTGAAACTAGCACTGGAGAGATCATTGGCAGGATGTCTGGGGACACCATTCTTATTCAATCAGCCATGGGGGAAAAGGTGGGGAAGTTTATCCAACTTTGTTCAACTTTTCTTGGTGGTTTTGTGATTGCTTTTGTCAAAGGGTGGCGCCTCACCGTTGTATTGCTAGCAACCATTCCTGCTATTGTCATTGCTGGTGCAATCATGGCTATTATCATGTCGAAAATGTCGTCTTGCGGACAGTCTGCTTATGCAGAAGCTGGCAATATAGTAGAACAAACAGTTGCATCTTTCACTGGGGAGAAGCAAGCAATTGAAAAGTACAACCAGAAGCTAAAAGTTGCCTACAATACTATGGTTCATCAAGGGCTAGTCACAGGGCTTGGACTTGGAATATTAATGCTTGTCATCTTCAGCTCATACGTGCTTGCGGTATGGTATGGAGCCCAATTGATCATCACGCGAGGATACAATGGAGGCCAAGTCATAAATGTTATATTTGCACTCATGATTGGTGGACACCGAAATCCAACTATCGATCCCTACGACACAAGTGGAATTCTATTGGAATATGTCAAAGGTGATGTCGAACTCAAAGACGTGTATTTCAGGTACCCTGCCAGACCAGCCGTACCGATCTTTGGTGGGTTCTCATTATATGTTCCAAGTGGCACAACCACCGCTTTGGTCGGGGAGAGTGGAAGCGGGAAGTCGACAGTGATTGGGCTAGTAGAAAGGTTTTATGATCCAGAAGCCGGTGAAGTACTTATAGATGGCGTGGACTTAAAGAAGTTTCAGCTTAAAGCGATAAGGGAGAAGATCGGGCTGGTTAGTCAGGAACCAAACCTCTTTACCACCACTATAAGGGAGAACATATCATATGGGAAGGATGATGCAACTGATGAGGAGATTAGAAGAGCAACTGAGCTTGCAAATGCTGCAAAATTCATTGATAAGCTACCCCAGGGAATCGACACAATGGTAGGTGAGCACGGGACATCTCTATCCGGTGGACAAAAGCAGAGAATTGCAATTGCAAGAGCCATTCTAAAGAACCCGAGAATTCTTCTTCTTGATGAAGCAACAAGCGCGCTGGATGCTGAGTCCGAAAAGATTGTTCAAGATGCATTGGTGAACTTGATGTCAAATTGCACAACCATAGTTGTTGCGCATCGGTTGACAACTATTAGGAATGCTGACTGCATAGCAGTGGTGCACAGAGGCAAAATTGTGGAAAAAGGGACTCATGATGAGTTGACCAAAGATCCAGAAGGGGCTTACAGCCAACTAATCCGCTTGCAAGAAGGATCGAAAGTGGACAACAAAACACAAACCTCTGATCCAAATAGAGTGGATACCAGCTTAGATATAGATAGGACTATGCTCAGCTGTGGGAACCAGATATTATCAATGAGGAGGTCTATAAGCAGAGGCTCACCAGGTAGCCGGCACTCATTCACTATTGGCTTTGGCATTCCCGGTCCAATTGATATACAAGAGAGTGAAGTAGAACACGAGGGCGATCATGACAGGCCTAAGGTTGATCCCAAAGAGCGCAAAACAGTTTCAATCCGAAGGCTGGCAGCCCTTTACAAACCTGAGCTTCCAGTTTTGCTGCTAGGATCCATTGCTGCAGCAGGGCATGGAGTAATTTTCCTCATCTTTGGCTTACTACTCACGAAAGCCATAAGAATTTTCTACGAACCACCCAATGAGCTTAGGCAGGATTGCAGAAAATGGGCATTAGTTTATGTAGGTTTAGCTTGCACAGGTCTGTTGAAGGTTGTGCACCAGCAAGTCAGTTGGTTTGATGACCCTGAAAATTCAAGCGGCGCCATTGGTGCAAGGTTATCTACAGATGCTTCGGTTATTAAGAGTCTTGTTGGTGACGCCTTAGCGTTGATTGTGCAGAATATAGCAACAATCATGGCAGGGTTCATTATAGGCTTCACAGCTAACTGGAAACTGGCGCTGATAGTTTTAGCCATTTCGCCGGTGATGATTCTGCAAGGAACACTTCAGGCAAAATTTCTTAAAGGGTTTAGTGCAGATGCCAAGGTGATGGAGGCATACGAGAAGAAGTGTGAGGGTACGGTTAGGAGTACTATTCTTGTGAAACATGGTCAAGCTACATTTGAACAAGTATTCATGCTGGCTCCTGACTTCAACAAAGCCAAGGACTCAGCAGCTTCGATATTTCAAATTCTCGACAGCAAGCCTAAGATTGACTCGAGCAGCGATGAAGGCATGACGCCATCACATTTAATTGGAGAAATCGAGCTTGAGCGTGTCAGCTTTAAGTATCCTACGCGCCCAGATGTGCAGATATTCAGAGACCTTTGTTTGAAGATGCCCTCTGGAAAGAGTGGCAGCGGGAAGTCGACGGTAATAGCCCTCATAGAGAGATTCTACGATCCTGATTCAGGGCGAGTGCTACTAGACGGAGTAGAAATCCAAAAGTTCAATCTAAATTGGCTGAGGCAGCAGATAGGGTTGGTTGGTCAAGAACTAGTTCTCTTCAACGAGTCCATTCGTTCCAACATTGCATACGGCAAGCCAGGAGATGTTACCGAGGAAGAGATCATAGCAGCAACCACGGCAGCTAACGTGCACAATGTCATATCTTCATTGCCTCAAGGCTACGACACCTCAGTAGGCGAACGAGGGGTTCAGCTATCAGGAGGTCAGAAGCAAAGAATCACCATTGCGAGGGCTATACTCAAGGACCCGAAAATCCTTCTACTTGATGAAGCTACGAGCGCACTGGATGCAGAGTCCGAACGCAAAGTGCAAGACGCATTGGACAGGGTTATGGTGAACAGAACAACCGTTGTAGTCGCGCACCGGCTTAGCACAATCAAAGGAGCAGACACGATCGCGGTGGTGAAGAATGGCGTGATTGCGGAGAAGGGAAGCCATGATTTCCTAATGAATATCGCTGACGGGGCTTACGCTTCTTTGGTGGCACTTCATTCCAGCTCGAACACATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
ko02010 ko04976 ko05206 ko05226 map02010 map04976 map05206 map05226
Pfam Domains
Protein Families
ABC_membrane ABC_tran

Protein Analysis

1091

Amino Acids

117.99

Weight (kDa)

6.83

Isoelectric Point (pI)

34.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ABC_membrane PF00664 1 - 266 6.8e-55 ABC transporter transmembrane region
ABC_tran PF00005 310 - 457 1.6e-33 ABC transporter
SMC_N PF02463 346 - 499 3.3e-06 RecF/RecN/SMC N terminal domain
ABC_membrane PF00664 688 - 779 2.2e-19 ABC transporter transmembrane region
ABC_tran PF00005 881 - 1014 1.7e-27 ABC transporter
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 1452
Acc65I GGTACC 1 cut(s) 894
AccB1I GGYRCC 3 cut(s) 429, 894, 2114
AccI GTMKAC 2 cut(s) 995, 2663
AccII CGCG 5 cut(s) 745, 1373, 2593, 3122, 3161
AcoI YGGCCR 1 cut(s) 153
AcsI RAATTY 7 cut(s) 840, 1226, 1344, 1974, 2104, 2309, 2484
AcuI CTGAAG 2 cut(s) 679, 2285
AcyI GRCGYC 4 cut(s) 430, 2115, 2172, 2534
AfaI GTAC 8 cut(s) 149, 242, 605, 896, 915, 1047, 2374, 2386
AfiI CCNNNNNNNGG 7 cut(s) 262, 923, 1037, 1146, 1861, 2705, 2991
AflIII ACRYGT 1 cut(s) 882
AhlI ACTAGT 1 cut(s) 2791
AjiI CACGTC 1 cut(s) 883
AjnI CCWGG 4 cut(s) 100, 1245, 1720, 2839
Alw21I GWGCWC 4 cut(s) 1275, 1500, 2073, 2895
Alw26I GTCTC 3 cut(s) 127, 138, 2610
Alw44I GTGCAC 3 cut(s) 1496, 2069, 2891
AlwNI CAGNNNCTG 1 cut(s) 542
Ama87I CYCGRG 2 cut(s) 1339, 2512
AoxI GGCC 3 cut(s) 153, 760, 1814
ApaLI GTGCAC 3 cut(s) 1496, 2069, 2891
ApoI RAATTY 7 cut(s) 840, 1226, 1344, 1974, 2104, 2309, 2484
ArsI GACNNNNNNTTYG 4 cut(s) 497, 529, 852, 884
AseI ATTAAT 1 cut(s) 680
Asp700I GAANNNNTTC 1 cut(s) 2793
Asp718I GGTACC 1 cut(s) 894
AspS9I GGNCC 2 cut(s) 1763, 3007
AsuC2I CCSGG 1 cut(s) 1761
AsuNHI GCTAGC 1 cut(s) 448
AvaI CYCGRG 2 cut(s) 1339, 2512
AvaII GGWCC 2 cut(s) 1763, 3007
AxyI CCTNAGG 2 cut(s) 257, 1818
BaeGI GKGCMC 3 cut(s) 1500, 2073, 2895
BalI TGGCCA 1 cut(s) 155
BamHI GGATCC 1 cut(s) 1904
BanI GGYRCC 3 cut(s) 429, 894, 2114
BanII GRGCYC 1 cut(s) 730
BauI CACGAG 1 cut(s) 1796
Bbv12I GWGCWC 4 cut(s) 1275, 1500, 2073, 2895
BbvCI CCTCAGC 1 cut(s) 2759
BccI CCATC 3 cut(s) 1049, 2338, 2545
BceAI ACGGC 4 cut(s) 8, 896, 2848, 2895
BciT130I CCWGG 4 cut(s) 102, 1247, 1722, 2841
BciVI GTATCC 3 cut(s) 743, 1630, 2595
BclI TGATCA 1 cut(s) 735
BcnI CCSGG 1 cut(s) 1761
BcoDI GTCTC 3 cut(s) 127, 138, 2610
BcuI ACTAGT 1 cut(s) 2791
BfaI CTAG 8 cut(s) 225, 291, 449, 653, 1010, 1901, 2720, 2792
BfmI CTRYAG 3 cut(s) 182, 1914, 2136
BfoI RGCGCY 3 cut(s) 433, 2118, 2255
BfuI GTATCC 3 cut(s) 743, 1630, 2595
BlpI GCTNAGC 2 cut(s) 1667, 3131
BmcAI AGTACT 2 cut(s) 1047, 2386
Bme1390I CCNGG 5 cut(s) 102, 1247, 1722, 1761, 2841
Bme18I GGWCC 2 cut(s) 1763, 3007
BmeT110I CYCGRG 2 cut(s) 1339, 2512
BmgBI CACGTC 1 cut(s) 883
BmgT120I GGNCC 2 cut(s) 1763, 3007
BmrFI CCNGG 5 cut(s) 102, 1247, 1722, 1761, 2841
BmrI ACTGGG 1 cut(s) 588
BmsI GCATC 9 cut(s) 578, 1165, 1369, 1396, 1462, 2131, 2323, 2622, 3040
BmtI GCTAGC 1 cut(s) 452
BmuI ACTGGG 1 cut(s) 588
BpmI CTGGAG 2 cut(s) 186, 318
Bpu10I CCTNAGC 4 cut(s) 1881, 2000, 2176, 2759
Bpu1102I GCTNAGC 2 cut(s) 1667, 3131
BpuEI CTTGAG 3 cut(s) 2585, 2901, 2987
BpuMI CCSGG 1 cut(s) 1761
Bsa29I ATCGAT 1 cut(s) 819
BsaAI YACGTR 1 cut(s) 706
BsaHI GRCGYC 4 cut(s) 430, 2115, 2172, 2534
BsaI GGTCTC 2 cut(s) 127, 2610
BsaJI CCNNGG 9 cut(s) 24, 117, 348, 1245, 1246, 2337, 2457, 2853, 2877
BsaWI WCCGGW 1 cut(s) 1289
BsaXI ACNNNNNCTCC 2 cut(s) 750, 780
Bsc4I CCNNNNNNNGG 7 cut(s) 262, 923, 1037, 1146, 1861, 2705, 2991
Bse118I RCCGGY 5 cut(s) 46, 1037, 1728, 2275, 3126
Bse1I ACTGG 7 cut(s) 169, 301, 583, 1889, 2246, 2253, 3051
Bse21I CCTNAGG 2 cut(s) 257, 1818
Bse3DI GCAATG 6 cut(s) 267, 471, 1908, 2823, 2909, 2985
BseBI CCWGG 4 cut(s) 102, 1247, 1722, 2841
BseCI ATCGAT 1 cut(s) 819
BseDI CCNNGG 9 cut(s) 24, 117, 348, 1245, 1246, 2337, 2457, 2853, 2877
BseGI GGATG 4 cut(s) 321, 1177, 1384, 3055
BseLI CCNNNNNNNGG 7 cut(s) 262, 923, 1037, 1146, 1861, 2705, 2991
BseMI GCAATG 6 cut(s) 267, 471, 1908, 2823, 2909, 2985
BseMII CTCAG 7 cut(s) 1197, 1374, 1681, 1872, 2478, 2750, 2946
BseNI ACTGG 7 cut(s) 169, 301, 583, 1889, 2246, 2253, 3051
BsePI GCGCGC 1 cut(s) 1371
BseRI GAGGAG 2 cut(s) 1202, 1711
BseSI GKGCMC 3 cut(s) 1500, 2073, 2895
BsgI GTGCAG 3 cut(s) 2210, 2349, 2624
Bsh1236I CGCG 5 cut(s) 745, 1373, 2593, 3122, 3161
Bsh1285I CGRYCG 1 cut(s) 3159
BshFI GGCC 3 cut(s) 155, 762, 1816
BshNI GGYRCC 3 cut(s) 429, 894, 2114
BshVI ATCGAT 1 cut(s) 819
BsiEI CGRYCG 1 cut(s) 3159
BsiHKAI GWGCWC 4 cut(s) 1275, 1500, 2073, 2895
BsiHKCI CYCGRG 2 cut(s) 1339, 2512
BsiSI CCGG 7 cut(s) 47, 1038, 1290, 1729, 1761, 2276, 3127
BslFI GGGAC 3 cut(s) 338, 1291, 1537
BslI CCNNNNNNNGG 7 cut(s) 262, 923, 1037, 1146, 1861, 2705, 2991
BsmAI GTCTC 3 cut(s) 127, 138, 2610
BsmFI GGGAC 3 cut(s) 338, 1291, 1537
BsmI GAATGC 2 cut(s) 1480, 1755
BsnI GGCC 3 cut(s) 155, 762, 1816
Bso31I GGTCTC 2 cut(s) 127, 2610
BsoBI CYCGRG 2 cut(s) 1339, 2512
Bsp1286I GDGCHC 5 cut(s) 730, 1275, 1500, 2073, 2895
Bsp1720I GCTNAGC 2 cut(s) 1667, 3131
Bsp19I CCATGG 2 cut(s) 117, 348
BspANI GGCC 3 cut(s) 155, 762, 1816
BspCNI CTCAG 7 cut(s) 1198, 1375, 1680, 1873, 2477, 2751, 2945
BspDI ATCGAT 1 cut(s) 819
BspFNI CGCG 5 cut(s) 745, 1373, 2593, 3122, 3161
BspHI TCATGA 4 cut(s) 51, 789, 1528, 1807
BspMAI CTGCAG 1 cut(s) 1918
BspOI GCTAGC 1 cut(s) 452
BspQI GCTCTTC 1 cut(s) 1192
BspT107I GGYRCC 3 cut(s) 429, 894, 2114
BspTNI GGTCTC 2 cut(s) 127, 2610
BsrDI GCAATG 6 cut(s) 267, 471, 1908, 2823, 2909, 2985
BsrFI RCCGGY 5 cut(s) 46, 1037, 1728, 2275, 3126
BsrI ACTGG 7 cut(s) 169, 301, 583, 1889, 2246, 2253, 3051
BssAI RCCGGY 5 cut(s) 46, 1037, 1728, 2275, 3126
BssECI CCNNGG 9 cut(s) 24, 117, 348, 1245, 1246, 2337, 2457, 2853, 2877
BssHII GCGCGC 1 cut(s) 1371
BssNI GRCGYC 4 cut(s) 430, 2115, 2172, 2534
BssSI CACGAG 1 cut(s) 1796
BssT1I CCWWGG 4 cut(s) 117, 348, 2337, 2457
Bst2BI CACGAG 1 cut(s) 1796
Bst2UI CCWGG 4 cut(s) 102, 1247, 1722, 2841
Bst4CI ACNGT 9 cut(s) 39, 439, 525, 565, 1000, 1846, 2377, 2668, 3112
Bst6I CTCTTC 3 cut(s) 1192, 2804, 2853
BstACI GRCGYC 4 cut(s) 430, 2115, 2172, 2534
BstAPI GCANNNNNTGC 2 cut(s) 1220, 3050
BstBAI YACGTR 1 cut(s) 706
BstDSI CCRYGG 4 cut(s) 24, 117, 348, 2877
BstENI CCTNNNNNAGG 2 cut(s) 260, 2703
BstF5I GGATG 4 cut(s) 321, 1177, 1384, 3055
BstFNI CGCG 5 cut(s) 745, 1373, 2593, 3122, 3161
BstH2I RGCGCY 3 cut(s) 433, 2118, 2255
BstMAI GTCTC 3 cut(s) 127, 138, 2610
BstMCI CGRYCG 1 cut(s) 3159
BstNI CCWGG 4 cut(s) 102, 1247, 1722, 2841
BstNSI RCATGY 1 cut(s) 195
BstSCI CCNGG 5 cut(s) 100, 1245, 1720, 1759, 2839
BstSFI CTRYAG 3 cut(s) 182, 1914, 2136
BstSLI GKGCMC 3 cut(s) 1500, 2073, 2895
BstUI CGCG 5 cut(s) 745, 1373, 2593, 3122, 3161
BstX2I RGATCY 2 cut(s) 1546, 1904
BstXI CCANNNNNNTGG 2 cut(s) 967, 2344
BstYI RGATCY 2 cut(s) 1546, 1904
Bsu15I ATCGAT 1 cut(s) 819
Bsu36I CCTNAGG 2 cut(s) 257, 1818
BsuI GTATCC 3 cut(s) 743, 1630, 2595
BsuRI GGCC 3 cut(s) 155, 762, 1816
BsuTUI ATCGAT 1 cut(s) 819
BtgI CCRYGG 4 cut(s) 24, 117, 348, 2877
BtgZI GCGATG 1 cut(s) 2535
BtrI CACGTC 1 cut(s) 883
BtsCI GGATG 4 cut(s) 321, 1177, 1384, 3055
BtsI GCAGTG 1 cut(s) 1497
BtsIMutI CAGTG 7 cut(s) 162, 282, 294, 576, 1005, 1497, 3044
CaiI CAGNNNCTG 1 cut(s) 542
CciI TCATGA 4 cut(s) 51, 789, 1528, 1807
Cfr10I RCCGGY 5 cut(s) 46, 1037, 1728, 2275, 3126
Cfr13I GGNCC 2 cut(s) 1763, 3007
ClaI ATCGAT 1 cut(s) 819
CseI GACGC 3 cut(s) 2180, 2542, 3086
CsiI ACCWGGT 1 cut(s) 1720
Csp6I GTAC 8 cut(s) 148, 241, 604, 895, 914, 1046, 2373, 2385
CspCI CAANNNNNGTGG 2 cut(s) 1614, 1649
CviQI GTAC 8 cut(s) 148, 241, 604, 895, 914, 1046, 2373, 2385
DinI GGCGCC 2 cut(s) 431, 2116
EaeI YGGCCR 1 cut(s) 153
Eam1104I CTCTTC 3 cut(s) 1192, 2804, 2853
EarI CTCTTC 3 cut(s) 1192, 2804, 2853
Eco130I CCWWGG 4 cut(s) 117, 348, 2337, 2457
Eco147I AGGCCT 1 cut(s) 1816
Eco24I GRGCYC 1 cut(s) 730
Eco31I GGTCTC 2 cut(s) 127, 2610
Eco47I GGWCC 2 cut(s) 1763, 3007
Eco57I CTGAAG 2 cut(s) 679, 2285
Eco81I CCTNAGG 2 cut(s) 257, 1818
Eco88I CYCGRG 2 cut(s) 1339, 2512
EcoNI CCTNNNNNAGG 2 cut(s) 260, 2703
EcoO109I RGGNCCY 1 cut(s) 3007
EcoRI GAATTC 2 cut(s) 840, 1344
EcoRII CCWGG 4 cut(s) 100, 1245, 1720, 2839
EcoT14I CCWWGG 4 cut(s) 117, 348, 2337, 2457
EcoT22I ATGCAT 1 cut(s) 1411
EcoT38I GRGCYC 1 cut(s) 730
EgeI GGCGCC 2 cut(s) 431, 2116
EheI GGCGCC 2 cut(s) 431, 2116
ErhI CCWWGG 4 cut(s) 117, 348, 2337, 2457
FalI AAGNNNNNCTT 2 cut(s) 2283, 2315
FaqI GGGAC 3 cut(s) 338, 1291, 1537
FauI CCCGC 2 cut(s) 980, 2648
FauNDI CATATG 1 cut(s) 1162
FbaI TGATCA 1 cut(s) 735
FblI GTMKAC 2 cut(s) 995, 2663
FokI GGATG 4 cut(s) 328, 1184, 1391, 3062
FriOI GRGCYC 1 cut(s) 730
FspBI CTAG 8 cut(s) 225, 291, 449, 653, 1010, 1901, 2720, 2792
FspI TGCGCA 1 cut(s) 1452
GsuI CTGGAG 2 cut(s) 186, 318
HaeII RGCGCY 3 cut(s) 433, 2118, 2255
HaeIII GGCC 3 cut(s) 155, 762, 1816
HapII CCGG 7 cut(s) 47, 1038, 1290, 1729, 1761, 2276, 3127
HgaI GACGC 3 cut(s) 2180, 2542, 3086
Hin1I GRCGYC 4 cut(s) 430, 2115, 2172, 2534
HincII GTYRAC 4 cut(s) 996, 1461, 1539, 2664
HindII GTYRAC 4 cut(s) 996, 1461, 1539, 2664
HpaII CCGG 7 cut(s) 47, 1038, 1290, 1729, 1761, 2276, 3127
Hpy188I TCNGA 6 cut(s) 1390, 1621, 1856, 2615, 2970, 3061
Hpy99I CGWCG 1 cut(s) 2668
HpyCH4III ACNGT 9 cut(s) 39, 439, 525, 565, 1000, 1846, 2377, 2668, 3112
HpyCH4IV ACGT 3 cut(s) 705, 882, 2889
HpySE526I ACGT 3 cut(s) 705, 882, 2889
Hsp92I GRCGYC 4 cut(s) 430, 2115, 2172, 2534
KasI GGCGCC 2 cut(s) 429, 2114
KpnI GGTACC 1 cut(s) 898
KroI GCCGGC 1 cut(s) 1728
KroNI GCCGGC 1 cut(s) 1730
Ksp22I TGATCA 1 cut(s) 735
LguI GCTCTTC 1 cut(s) 1192
LmnI GCTCC 4 cut(s) 167, 725, 2443, 3146
LweI GCATC 9 cut(s) 578, 1165, 1369, 1396, 1462, 2131, 2323, 2622, 3040
MabI ACCWGGT 1 cut(s) 1720
MaeI CTAG 8 cut(s) 225, 291, 449, 653, 1010, 1901, 2720, 2792
MaeII ACGT 3 cut(s) 705, 882, 2889
MaeIII GTNAC 4 cut(s) 202, 655, 2168, 2848
MfeI CAATTG 4 cut(s) 594, 731, 1314, 1767
MflI RGATCY 2 cut(s) 1546, 1904
MhlI GDGCHC 5 cut(s) 730, 1275, 1500, 2073, 2895
MlsI TGGCCA 1 cut(s) 155
MluNI TGGCCA 1 cut(s) 155
Mly113I GGCGCC 2 cut(s) 430, 2115
MlyI GAGTC 8 cut(s) 49, 1394, 1519, 2167, 2456, 2504, 2816, 3065
MmeI TCCRAC 3 cut(s) 400, 836, 2844
Mox20I TGGCCA 1 cut(s) 155
Mph1103I ATGCAT 1 cut(s) 1411
MroNI GCCGGC 1 cut(s) 1728
MroXI GAANNNNTTC 1 cut(s) 2793
MscI TGGCCA 1 cut(s) 155
MseI TTAA 7 cut(s) 680, 1067, 1083, 2154, 2316, 2546, 2580
MslI CAYNNNNRTG 1 cut(s) 1490
Msp20I TGGCCA 1 cut(s) 155
MspA1I CMGCKG 2 cut(s) 1671, 2655
MspI CCGG 7 cut(s) 47, 1038, 1290, 1729, 1761, 2276, 3127
MspR9I CCNGG 5 cut(s) 102, 1247, 1722, 1761, 2841
MunI CAATTG 4 cut(s) 594, 731, 1314, 1767
Mva1269I GAATGC 2 cut(s) 1480, 1755
MvaI CCWGG 4 cut(s) 102, 1247, 1722, 2841
MvnI CGCG 5 cut(s) 745, 1373, 2593, 3122, 3161
NaeI GCCGGC 1 cut(s) 1730
NarI GGCGCC 2 cut(s) 430, 2115
NciI CCSGG 1 cut(s) 1761
NcoI CCATGG 2 cut(s) 117, 348
NdeI CATATG 1 cut(s) 1162
NgoMIV GCCGGC 1 cut(s) 1728
NheI GCTAGC 1 cut(s) 448
NmuCI GTSAC 3 cut(s) 202, 655, 2168
NsbI TGCGCA 1 cut(s) 1452
NsiI ATGCAT 1 cut(s) 1411
NspI RCATGY 1 cut(s) 195
PaeR7I CTCGAG 1 cut(s) 2512
PagI TCATGA 4 cut(s) 51, 789, 1528, 1807
PasI CCCWGGG 1 cut(s) 1246
PauI GCGCGC 1 cut(s) 1371
PceI AGGCCT 1 cut(s) 1816
PciSI GCTCTTC 1 cut(s) 1192
PcsI WCGNNNNNNNCGW 2 cut(s) 509, 825
PctI GAATGC 2 cut(s) 1480, 1755
PdiI GCCGGC 1 cut(s) 1730
PdmI GAANNNNTTC 1 cut(s) 2793
PfeI GAWTC 5 cut(s) 1251, 2284, 2688, 2702, 2979
Ple19I CGATCG 1 cut(s) 3159
PleI GAGTC 8 cut(s) 49, 1393, 1519, 2166, 2456, 2504, 2815, 3064
PluTI GGCGCC 2 cut(s) 433, 2118
PpsI GAGTC 8 cut(s) 49, 1393, 1519, 2166, 2456, 2504, 2815, 3064
Ppu21I YACGTR 1 cut(s) 706
PpuMI RGGWCCY 1 cut(s) 3007
PshBI ATTAAT 1 cut(s) 680
Psp5II RGGWCCY 1 cut(s) 3007
Psp6I CCWGG 4 cut(s) 100, 1245, 1720, 2839
PspGI CCWGG 4 cut(s) 100, 1245, 1720, 2839
PspPI GGNCC 2 cut(s) 1763, 3007
PspPPI RGGWCCY 1 cut(s) 3007
PspXI VCTCGAGB 1 cut(s) 2512
PstI CTGCAG 1 cut(s) 1918
PstNI CAGNNNCTG 1 cut(s) 542
PsuI RGATCY 2 cut(s) 1546, 1904
PteI GCGCGC 1 cut(s) 1371
PvuI CGATCG 1 cut(s) 3159
PvuII CAGCTG 1 cut(s) 1671
RsaI GTAC 8 cut(s) 149, 242, 605, 896, 915, 1047, 2374, 2386
RsaNI GTAC 8 cut(s) 148, 241, 604, 895, 914, 1046, 2373, 2385
RseI CAYNNNNRTG 1 cut(s) 1490
SalI GTCGAC 2 cut(s) 994, 2662
SapI GCTCTTC 1 cut(s) 1192
SaqAI TTAA 7 cut(s) 680, 1067, 1083, 2154, 2316, 2546, 2580
Sau96I GGNCC 2 cut(s) 1763, 3007
ScaI AGTACT 2 cut(s) 1047, 2386
SchI GAGTC 8 cut(s) 49, 1394, 1519, 2167, 2456, 2504, 2816, 3065
ScrFI CCNGG 5 cut(s) 102, 1247, 1722, 1761, 2841
SduI GDGCHC 5 cut(s) 730, 1275, 1500, 2073, 2895
SexAI ACCWGGT 1 cut(s) 1720
SfaNI GCATC 9 cut(s) 578, 1165, 1369, 1396, 1462, 2131, 2323, 2622, 3040
SfcI CTRYAG 3 cut(s) 182, 1914, 2136
SfoI GGCGCC 2 cut(s) 431, 2116
Sfr274I CTCGAG 1 cut(s) 2512
SgrAI CRCCGGYG 1 cut(s) 2275
SinI GGWCC 2 cut(s) 1763, 3007
SlaI CTCGAG 1 cut(s) 2512
SmiMI CAYNNNNRTG 1 cut(s) 1490
SmlI CTYRAG 4 cut(s) 2512, 2564, 2916, 3002
SmoI CTYRAG 4 cut(s) 2512, 2564, 2916, 3002
SpeI ACTAGT 1 cut(s) 2791
SseBI AGGCCT 1 cut(s) 1816
SspDI GGCGCC 2 cut(s) 429, 2114
SspI AATATT 1 cut(s) 678
SspMI CTAG 8 cut(s) 225, 291, 449, 653, 1010, 1901, 2720, 2792
StuI AGGCCT 1 cut(s) 1816
StyD4I CCNGG 5 cut(s) 100, 1245, 1720, 1759, 2839
StyI CCWWGG 4 cut(s) 117, 348, 2337, 2457
TaaI ACNGT 9 cut(s) 39, 439, 525, 565, 1000, 1846, 2377, 2668, 3112
TaiI ACGT 3 cut(s) 708, 885, 2892
TatI WGTACW 5 cut(s) 147, 240, 603, 1045, 2384
TauI GCSGC 3 cut(s) 46, 176, 2115
TfiI GAWTC 5 cut(s) 1251, 2284, 2688, 2702, 2979
Tru1I TTAA 7 cut(s) 680, 1067, 1083, 2154, 2316, 2546, 2580
Tru9I TTAA 7 cut(s) 680, 1067, 1083, 2154, 2316, 2546, 2580
TscAI CASTG 7 cut(s) 169, 289, 301, 583, 1005, 1497, 3051
TseFI GTSAC 3 cut(s) 202, 655, 2168
Tsp45I GTSAC 3 cut(s) 202, 655, 2168
TspGWI ACGGA 1 cut(s) 2739
TspRI CASTG 7 cut(s) 169, 289, 301, 583, 1005, 1497, 3051
VneI GTGCAC 3 cut(s) 1496, 2069, 2891
VpaK11BI GGWCC 2 cut(s) 1763, 3007
VspI ATTAAT 1 cut(s) 680
XagI CCTNNNNNAGG 2 cut(s) 260, 2703
XapI RAATTY 7 cut(s) 840, 1226, 1344, 1974, 2104, 2309, 2484
XceI RCATGY 1 cut(s) 195
XhoI CTCGAG 1 cut(s) 2512
XmiI GTMKAC 2 cut(s) 995, 2663
XmnI GAANNNNTTC 1 cut(s) 2793
XspI CTAG 8 cut(s) 225, 291, 449, 653, 1010, 1901, 2720, 2792
ZrmI AGTACT 2 cut(s) 1047, 2386
Zsp2I ATGCAT 1 cut(s) 1411
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.