Rw3G017340

ABC transporter B family member

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr3
OG0001944
Physical Location & Seq
Reverse (-)
18743623 .. 18748934
5312 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw3G017340.1

Sequence Viewer

Length: 3765 bp
ATGGAGCGAGGAGGAGAGATGACAATGAATAAGAAAAAAGCAAGTGATCAAGACAAGCAGAGTGTTCCATTTTACAAGCTGTTTATGTTCGCTGACCAATACGACATCATGTTGATGATCATCGGAAGTTTAAGTGCGATTGGAAATGGGCTGTCACAGCCACTCATGACGCTGGTCTTCGGTGGTCTTATCAATTCTTTTGGAGCTACCGACCCAGCTCATATTGTCCCTACTGTTTCTCAAGTGTCTCTAAAGTTTTTGTACTTGGCTATTGGTACAGGCCTTGCTGCATTTTTGCAGGTCTCATGTTGGATGGTGACTGGGGAAAGACAAGCCACTCGAATTCGAGGCTTGTACTTGAAAACAATACTAAGACAGGATATTGGATTTTTTGACACTGAAACTAACACCGGGAGATCATTGGGAGAATGTCAGGGGACACCATTCTCATTCAAAATGCCATGGGCGAAAAGGAACGTGGGGAAGTTCATACAGCTCCTTTCAACTTTTCTCGGCGGTTTTGTAATTGCTTTTCTCAAAGGATGGCTTCTCACCTTGGTTTTGCTGTCCTGTATTCCTGCAATTGTTATTGCTGGAGGAGTCATGTCCATTGTCGTTTCTAAAATGTCAACACGCGGGCAGCGTGCTTATGCAGAAGCTGGCACTATAGTAGAACAAACTGTTGGAGCCATCAGAACAGTTGCATCTTTTACCGGAGAGAAGGAAGCGATAGATAAATACAACCAGAAGCTGAAAATTGCGTACAATAACACGGTTCAACAAGGACTAGCCACAGGGGTTGGACTTGGTACATTCATGCTCATTGTCTTTGGCACTTATGGACTTGCAATATGGTATGGATCAAAAATGATCATTGAGAAAGGCTACAATGGGGGCCAAGTCATCAATGTTCTATTTGCAATGATGAGTGGTGGAATGGCACTAGGACAGACATCACCGTCTCTTAATGCATTTACAACAGGAAAAGCTGCTGCATATAAAATGCTTGAGACCATCAAGCGAACACCAACAATTGATCCTTATGATACTACTGGAGTTGTATTGGAAGATGTTAAAGGAGAGGTCGAACTTAAAGATGTGTATTTCAGGTATCCTGCCAGGCCAGATGTGCAGATCTTTGCTGGATTCTCGTTGCATGTTCCGAGCGGCACAACGACTGCTTTGGTTGGGCAGAGTGGAAGTGGGAAGTCAACAGTAATAGGCTTGGTAGAGAGGTTTTATGATCCAGAAGCTGGTGAAGTGTTTATAGATGGAGTCGATTTGAAGAAGTTGCATCTCAGATCAATAAGGGAGAGGATTGGGCTTGTAAGTCAAGAGCCAAATCTTTTTACAACAACAATAAGGCAGAACATAGCATATGGGAAAGATAATGCAACCGAAGAGGAGATTAGAAGAGCCCTGGAGCTTGCAAATGCAGCAAAGTTCATTGATAAACTGCCCAAGGGGCTTGACACAATGGTAGGTGGAACATCATTATCGGGTGGACAAAAGCAAAGAATTGCAATTGCTAGAGCCATTTTGAAGAACCCAAGAATTCTGCTTCTTGACGAAGCAACAAGCGCTCTAGATACTGAGTCTGAACGAATAGTTCAAGATGCACTTGTCAGATTAATGTCAAATAGGACAACTATAGTTGTTGCTCATCGCTTGACAACGATAAGAAATGCTGATGCAATAGCAGTGGTTCATAAAGGGAAAATTGTAGAAAAAGGAACTCACGACGAATTGACCAAAGATCCAGAAGGAGCTTACAGCCAACTACTTCGTCTACAGGAAGGAGCTAAAGAAAAAGATGTAGAATCGACCGGTCTAGATAATGGTTTAGATATAGATAAGACCATGCTTAGCTCTGGGAGCCAGAGATTATCAGGAGGGGCATCTTTAAGCAGAGGTTCGTCAGGTAGCCGGCTGTCCACCACAATTAGCAGCTTTGGCACTCCTGCTCTGGTTAATTGCCAAGTGACTGAAGTAGTTGATCCCGAAGAGCGTAAAGCAGTTTCCATCAAAAGGTTGGCCATGATGAACAAAAATGAGCTTCCAATTTTGCTTATTGGAGCCATTGCTGCAGCTGGTCATGGAGTAATTTTTCCTGTATTCGGCTTACTACTCTCAAAAGCCATTAAAATGTTCTATGAACCGCATAGTGTGCTACGAGAAGACTCGAAAACATGGGCTGGAGTGTATGTAGGGATGGGTTGCCTTGGGCTGGTTGTCGTTCCGGTGCAGAATTTCTTCTTTGGAGTTGCAGGTGGAAAATTGATACAGCAAATTCGTTCCTTGACATTTCAAAAGGTGGTGCACCAAGAAATTAGTTGGTTTGATGATCCCGCAAATTCAAGTGGTGCCATTGGTGCAAGGTTGTCTTCTGATGCTTCAACCGTGAAGGCTCTTGTTGGTGACGGCTTAGCCTTGATTGCACAAAATATAGCAACTGTCATAGCAGGGCTGGTTATAGGGTTCACAGCTAACTGGAAACTGGCGCTTCTAATTTTAGGCGTGTCGCCATTATTGATTATGCAAGGAGTACTTCAAACCAAATTTCTTAAAGGGTTTAGTGGAGATGCCAAGGTTATGTACGAAGAAGCTAGCCAAGTGGCAAATGATGCAATTGGTAGCATTCGAACTGTTGCATCATTTTGTTCGGAAAAGAAGGTGATGGATGCTTATGAGAAGAAGTGTGAAGGTCCCATGAAGCAAGGAGTACGGTTAGGAGTAGTGAGTGGTGTAGGTTTTGGCTTCTCTTTCTTCGTAATGTTCTGCACCAATGCATTAATTTTCTACATTGGAGCTATTCTTGTAAAAAATGGGCAAGCTAAATTTGAACAAGTATTCATGGTGTTCTTTGCTTTGACAATCTCAGCAGTAGGAGTTTCAGAATCTAGTGGTATGGCTCCTGACTCCAACAAAGCAAAGGACTCAGCAGCTTCAATATTTAAGATTCTTGACAGTAAGCCTAAGATCGACTCGAGCAGCGATGCAGGCTTAACACTACCGAGCATAAGTGGAAAAATTGATCTCGAACATGTCAGCTTCAAATATCCCACAAGGCCGGATGTGCAGATATTTAGAGACGTTTGCTTGAATTTCCCCTCTGGAAAGACTGTTGCTTTGGTTGGAGAGAGTGGCAGTGGGAAGTCGACAGTGATTGGCCTCATAGAGAGATTCTATGACCCCGACTCAGGTCGTGTACTATTAGATGGAGTGGAAATCAATAGGTTCAAGATAAACTGGCTGAGGCAACAAATAGGTTTGGTTGGTCAAGAACCAATTCTCTTTAACGCATCCATTCGGACCAATATTGCTTATGGCAAGCGAGGAGATGTAACTGAAGAACAGATCATTGCAGCCACAAAGGCATCAAATGCTCACAACTTCATTTCTTCATTGCCTCAAGGATATGACACCTCAGTTGGGGAACGAGGGGTTCAGTTATCTGGAGGGCAGAAGCAAAGAATAGCCATTGCAAGGGCTATTCTAAAGGACCCGAAAATTCTTTTGCTTGACGAAGCTACAAGCGCACTGGATGCAGAGTCGGAACGCATAGTACAAGATGCTTTAGACAGAGTGATGGTGAATAGAACAACTGTTGTCGTTGCGCACCGCCTTACCACGATCAAAGGGGCAGACATAATTGCTGTGGTCAAGAACGGCGTGATTGCTGAGAAGGGTAGCCATGATTTTCTAATGAAGATCACTGATGGTGCTTATGCGTCTTTGGTGGCACTTCATTCAAGCTCAACATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
ko02010 ko04976 ko05206 ko05226 map02010 map04976 map05206 map05226
Pfam Domains
Protein Families
ABC_membrane ABC_tran

Protein Analysis

1254

Amino Acids

134.99

Weight (kDa)

9.0

Isoelectric Point (pI)

32.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ABC_membrane PF00664 39 - 311 1.6e-48 ABC transporter transmembrane region
ABC_tran PF00005 382 - 527 1.1e-31 ABC transporter
ABC_membrane PF00664 689 - 961 1.1e-59 ABC transporter transmembrane region
ABC_tran PF00005 1030 - 1179 1.5e-32 ABC transporter
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 2258
AasI GACNNNNNNGTC 1 cut(s) 958
Acc16I TGCGCA 1 cut(s) 3618
Acc36I ACCTGC 2 cut(s) 289, 2258
AccB1I GGYRCC 1 cut(s) 2363
AccB7I CCANNNNNTGG 1 cut(s) 1253
AccBSI CCGCTC 1 cut(s) 1167
AccI GTMKAC 2 cut(s) 1789, 3158
AccII CGCG 1 cut(s) 636
AciI CCGC 6 cut(s) 516, 636, 1167, 2159, 2349, 3622
AclWI GGATC 6 cut(s) 868, 1031, 1238, 1751, 1991, 2339
AcoI YGGCCR 1 cut(s) 2034
AcsI RAATTY 9 cut(s) 342, 1554, 2248, 2289, 2353, 2558, 2837, 3103, 3510
AcuI CTGAAG 2 cut(s) 2007, 3369
AfeI AGCGCT 1 cut(s) 1582
AfiI CCNNNNNNNGG 7 cut(s) 1253, 2028, 2117, 2227, 3200, 3432, 3486
AflIII ACRYGT 1 cut(s) 3043
AgeI ACCGGT 1 cut(s) 1826
AjnI CCWGG 2 cut(s) 1118, 1419
AjuI GAANNNNNNNTTGG 2 cut(s) 666, 698
Alw21I GWGCWC 1 cut(s) 2322
Alw26I GTCTC 5 cut(s) 252, 307, 966, 1004, 3084
Alw44I GTGCAC 1 cut(s) 2318
AlwI GGATC 6 cut(s) 868, 1031, 1238, 1751, 1991, 2339
AlwNI CAGNNNCTG 3 cut(s) 659, 751, 1253
Ama87I CYCGRG 1 cut(s) 2986
Aor51HI AGCGCT 1 cut(s) 1582
AoxI GGCC 6 cut(s) 280, 895, 1121, 2034, 3068, 3169
ApaLI GTGCAC 1 cut(s) 2318
ApoI RAATTY 9 cut(s) 342, 1554, 2248, 2289, 2353, 2558, 2837, 3103, 3510
ArsI GACNNNNNNTTYG 2 cut(s) 3112, 3144
AseI ATTAAT 2 cut(s) 1631, 2792
AsiGI ACCGGT 1 cut(s) 1826
Asp700I GAANNNNTTC 2 cut(s) 2252, 3288
AspLEI GCGC 4 cut(s) 1583, 2503, 3539, 3619
AspS9I GGNCC 4 cut(s) 895, 2705, 3312, 3502
AsuC2I CCSGG 1 cut(s) 412
AsuHPI GGTGA 7 cut(s) 328, 544, 948, 1268, 2429, 2686, 3604
AsuII TTCGAA 1 cut(s) 2641
AsuNHI GCTAGC 1 cut(s) 2606
AvaI CYCGRG 1 cut(s) 2986
AvaII GGWCC 3 cut(s) 2705, 3312, 3502
BaeGI GKGCMC 1 cut(s) 2322
BalI TGGCCA 1 cut(s) 2036
BanI GGYRCC 1 cut(s) 2363
BanII GRGCYC 1 cut(s) 1420
BbsI GAAGAC 3 cut(s) 169, 2184, 2376
Bbv12I GWGCWC 1 cut(s) 2322
BbvCI CCTCAGC 1 cut(s) 3254
BceAI ACGGC 2 cut(s) 2437, 3685
BciT130I CCWGG 2 cut(s) 1120, 1421
BciVI GTATCC 1 cut(s) 1122
BclI TGATCA 3 cut(s) 46, 117, 870
BcnI CCSGG 1 cut(s) 412
BcoDI GTCTC 5 cut(s) 252, 307, 966, 1004, 3084
BfaI CTAG 7 cut(s) 788, 944, 1530, 1586, 1832, 2607, 2901
BfmI CTRYAG 4 cut(s) 666, 1650, 1790, 2085
BfoI RGCGCY 2 cut(s) 1584, 2504
BfuAI ACCTGC 2 cut(s) 289, 2258
BfuI GTATCC 1 cut(s) 1122
BglI GCCNNNNNGGC 2 cut(s) 1465, 3374
BglII AGATCT 1 cut(s) 1134
BlpI GCTNAGC 2 cut(s) 1865, 2425
BmcAI AGTACT 1 cut(s) 2547
Bme1390I CCNGG 3 cut(s) 412, 1120, 1421
Bme18I GGWCC 3 cut(s) 2705, 3312, 3502
BmeT110I CYCGRG 1 cut(s) 2986
BmgT120I GGNCC 4 cut(s) 895, 2705, 3312, 3502
BmiI GGNNCC 8 cut(s) 688, 896, 1877, 2077, 2365, 2707, 2913, 3504
BmrFI CCNGG 3 cut(s) 412, 1120, 1421
BmrI ACTGGG 1 cut(s) 330
BmtI GCTAGC 1 cut(s) 2610
BmuI ACTGGG 1 cut(s) 330
BoxI GACNNNNGTC 2 cut(s) 173, 3201
BpiI GAAGAC 3 cut(s) 169, 2184, 2376
BpmI CTGGAG 5 cut(s) 615, 1074, 1442, 2217, 3477
Bpu10I CCTNAGC 1 cut(s) 3254
Bpu1102I GCTNAGC 2 cut(s) 1865, 2425
Bpu14I TTCGAA 1 cut(s) 2641
BpuEI CTTGAG 3 cut(s) 225, 1028, 3396
BpuMI CCSGG 1 cut(s) 412
BsaBI GATNNNNATC 1 cut(s) 119
BsaI GGTCTC 2 cut(s) 307, 1004
BsaJI CCNNGG 6 cut(s) 461, 555, 1419, 1461, 2221, 2586
BsaWI WCCGGW 3 cut(s) 713, 1826, 2239
BsaXI ACNNNNNCTCC 2 cut(s) 2190, 2220
Bsc4I CCNNNNNNNGG 7 cut(s) 1253, 2028, 2117, 2227, 3200, 3432, 3486
Bse118I RCCGGY 2 cut(s) 1826, 1926
Bse1I ACTGG 6 cut(s) 325, 1057, 2495, 2502, 3254, 3546
Bse3DI GCAATG 5 cut(s) 927, 2079, 3360, 3404, 3480
Bse8I GATNNNNATC 1 cut(s) 119
BseBI CCWGG 2 cut(s) 1120, 1421
BseDI CCNNGG 6 cut(s) 461, 555, 1419, 1461, 2221, 2586
BseGI GGATG 7 cut(s) 318, 548, 2217, 2686, 3079, 3302, 3550
BseJI GATNNNNATC 1 cut(s) 119
BseLI CCNNNNNNNGG 7 cut(s) 1253, 2028, 2117, 2227, 3200, 3432, 3486
BseMI GCAATG 5 cut(s) 927, 2079, 3360, 3404, 3480
BseMII CTCAG 8 cut(s) 1312, 1584, 2892, 2952, 3213, 3245, 3441, 3672
BseNI ACTGG 6 cut(s) 325, 1057, 2495, 2502, 3254, 3546
BseRI GAGGAG 5 cut(s) 24, 27, 612, 1418, 3351
BseSI GKGCMC 1 cut(s) 2322
BseYI CCCAGC 1 cut(s) 214
BsgI GTGCAG 4 cut(s) 1151, 2264, 2764, 3098
Bsh1236I CGCG 1 cut(s) 636
Bsh1285I CGRYCG 1 cut(s) 1827
BshFI GGCC 6 cut(s) 282, 897, 1123, 2036, 3070, 3171
BshNI GGYRCC 1 cut(s) 2363
BshTI ACCGGT 1 cut(s) 1826
BsiEI CGRYCG 1 cut(s) 1827
BsiHKAI GWGCWC 1 cut(s) 2322
BsiHKCI CYCGRG 1 cut(s) 2986
BsiSI CCGG 6 cut(s) 411, 714, 1827, 1927, 2240, 3071
BslFI GGGAC 3 cut(s) 212, 451, 2691
BslI CCNNNNNNNGG 7 cut(s) 1253, 2028, 2117, 2227, 3200, 3432, 3486
BsmAI GTCTC 5 cut(s) 252, 307, 966, 1004, 3084
BsmBI CGTCTC 2 cut(s) 966, 3084
BsmFI GGGAC 3 cut(s) 212, 451, 2691
BsmI GAATGC 1 cut(s) 2637
BsnI GGCC 6 cut(s) 282, 897, 1123, 2036, 3070, 3171
Bso31I GGTCTC 2 cut(s) 307, 1004
BsoBI CYCGRG 1 cut(s) 2986
Bsp119I TTCGAA 1 cut(s) 2641
Bsp1286I GDGCHC 2 cut(s) 1420, 2322
Bsp1720I GCTNAGC 2 cut(s) 1865, 2425
Bsp19I CCATGG 1 cut(s) 461
BspACI CCGC 6 cut(s) 516, 636, 1167, 2159, 2349, 3622
BspANI GGCC 6 cut(s) 282, 897, 1123, 2036, 3070, 3171
BspCNI CTCAG 8 cut(s) 1311, 1585, 2891, 2951, 3212, 3246, 3440, 3673
BspFNI CGCG 1 cut(s) 636
BspHI TCATGA 1 cut(s) 165
BspLI GGNNCC 8 cut(s) 688, 896, 1877, 2077, 2365, 2707, 2913, 3504
BspMAI CTGCAG 1 cut(s) 2089
BspMI ACCTGC 2 cut(s) 289, 2258
BspOI GCTAGC 1 cut(s) 2610
BspPI GGATC 6 cut(s) 868, 1031, 1238, 1751, 1991, 2339
BspQI GCTCTTC 2 cut(s) 1408, 1998
BspT104I TTCGAA 1 cut(s) 2641
BspT107I GGYRCC 1 cut(s) 2363
BspTNI GGTCTC 2 cut(s) 307, 1004
BsrBI CCGCTC 1 cut(s) 1167
BsrDI GCAATG 5 cut(s) 927, 2079, 3360, 3404, 3480
BsrFI RCCGGY 2 cut(s) 1826, 1926
BsrI ACTGG 6 cut(s) 325, 1057, 2495, 2502, 3254, 3546
BssAI RCCGGY 2 cut(s) 1826, 1926
BssECI CCNNGG 6 cut(s) 461, 555, 1419, 1461, 2221, 2586
BssT1I CCWWGG 5 cut(s) 461, 555, 1461, 2221, 2586
Bst2UI CCWGG 2 cut(s) 1120, 1421
Bst6I CTCTTC 3 cut(s) 1395, 1408, 1998
BstAPI GCANNNNNTGC 4 cut(s) 2167, 2624, 3383, 3545
BstBI TTCGAA 1 cut(s) 2641
BstC8I GCNNGC 9 cut(s) 638, 645, 661, 1428, 1928, 2608, 2832, 3001, 3332
BstDSI CCRYGG 1 cut(s) 461
BstF5I GGATG 7 cut(s) 318, 548, 2217, 2686, 3079, 3302, 3550
BstFNI CGCG 1 cut(s) 636
BstH2I RGCGCY 2 cut(s) 1584, 2504
BstHHI GCGC 4 cut(s) 1583, 2503, 3539, 3619
BstMAI GTCTC 5 cut(s) 252, 307, 966, 1004, 3084
BstMCI CGRYCG 1 cut(s) 1827
BstNI CCWGG 2 cut(s) 1120, 1421
BstNSI RCATGY 2 cut(s) 1160, 3047
BstPAI GACNNNNGTC 2 cut(s) 173, 3201
BstSCI CCNGG 3 cut(s) 410, 1118, 1419
BstSFI CTRYAG 4 cut(s) 666, 1650, 1790, 2085
BstSLI GKGCMC 1 cut(s) 2322
BstUI CGCG 1 cut(s) 636
BstV2I GAAGAC 3 cut(s) 169, 2184, 2376
BstX2I RGATCY 2 cut(s) 1134, 1756
BstYI RGATCY 2 cut(s) 1134, 1756
BsuI GTATCC 1 cut(s) 1122
BsuRI GGCC 6 cut(s) 282, 897, 1123, 2036, 3070, 3171
BtgI CCRYGG 1 cut(s) 461
BtgZI GCGATG 2 cut(s) 1649, 3009
BtsCI GGATG 7 cut(s) 318, 548, 2217, 2686, 3079, 3302, 3550
BtsI GCAGTG 2 cut(s) 1707, 3154
BtsIMutI CAGTG 6 cut(s) 396, 1707, 3154, 3168, 3539, 3714
BveI ACCTGC 2 cut(s) 289, 2258
Cac8I GCNNGC 9 cut(s) 638, 645, 661, 1428, 1928, 2608, 2832, 3001, 3332
CaiI CAGNNNCTG 3 cut(s) 659, 751, 1253
CciI TCATGA 1 cut(s) 165
CfoI GCGC 4 cut(s) 1583, 2503, 3539, 3619
Cfr10I RCCGGY 2 cut(s) 1826, 1926
Cfr13I GGNCC 4 cut(s) 895, 2705, 3312, 3502
CseI GACGC 2 cut(s) 178, 3720
CspAI ACCGGT 1 cut(s) 1826
CspCI CAANNNNNGTGG 4 cut(s) 781, 816, 1683, 1718
DrdI GACNNNNNNGTC 1 cut(s) 958
DseDI GACNNNNNNGTC 1 cut(s) 958
EaeI YGGCCR 1 cut(s) 2034
Eam1104I CTCTTC 3 cut(s) 1395, 1408, 1998
EarI CTCTTC 3 cut(s) 1395, 1408, 1998
Eco130I CCWWGG 5 cut(s) 461, 555, 1461, 2221, 2586
Eco147I AGGCCT 1 cut(s) 282
Eco24I GRGCYC 1 cut(s) 1420
Eco31I GGTCTC 2 cut(s) 307, 1004
Eco47I GGWCC 3 cut(s) 2705, 3312, 3502
Eco47III AGCGCT 1 cut(s) 1582
Eco57I CTGAAG 2 cut(s) 2007, 3369
Eco88I CYCGRG 1 cut(s) 2986
EcoO109I RGGNCCY 2 cut(s) 2705, 3502
EcoRI GAATTC 2 cut(s) 342, 1554
EcoRII CCWGG 2 cut(s) 1118, 1419
EcoT14I CCWWGG 5 cut(s) 461, 555, 1461, 2221, 2586
EcoT22I ATGCAT 2 cut(s) 973, 2791
EcoT38I GRGCYC 1 cut(s) 1420
ErhI CCWWGG 5 cut(s) 461, 555, 1461, 2221, 2586
Esp3I CGTCTC 2 cut(s) 966, 3084
FalI AAGNNNNNCTT 8 cut(s) 531, 563, 1605, 1637, 2368, 2400, 2532, 2564
FaqI GGGAC 3 cut(s) 212, 451, 2691
FauI CCCGC 2 cut(s) 629, 2356
FauNDI CATATG 1 cut(s) 1378
FbaI TGATCA 3 cut(s) 46, 117, 870
FblI GTMKAC 2 cut(s) 1789, 3158
FokI GGATG 7 cut(s) 325, 555, 2224, 2693, 3086, 3289, 3557
FriOI GRGCYC 1 cut(s) 1420
FspBI CTAG 7 cut(s) 788, 944, 1530, 1586, 1832, 2607, 2901
FspI TGCGCA 1 cut(s) 3618
GlaI GCGC 4 cut(s) 1582, 2502, 3538, 3618
GsaI CCCAGC 1 cut(s) 218
GsuI CTGGAG 5 cut(s) 615, 1074, 1442, 2217, 3477
HaeII RGCGCY 2 cut(s) 1584, 2504
HaeIII GGCC 6 cut(s) 282, 897, 1123, 2036, 3070, 3171
HapII CCGG 6 cut(s) 411, 714, 1827, 1927, 2240, 3071
HgaI GACGC 2 cut(s) 178, 3720
HhaI GCGC 4 cut(s) 1583, 2503, 3539, 3619
Hin6I GCGC 4 cut(s) 1581, 2501, 3537, 3617
HinP1I GCGC 4 cut(s) 1581, 2501, 3537, 3617
HincII GTYRAC 3 cut(s) 630, 1212, 3159
HindII GTYRAC 3 cut(s) 630, 1212, 3159
HpaII CCGG 6 cut(s) 411, 714, 1827, 1927, 2240, 3071
HphI GGTGA 7 cut(s) 328, 544, 948, 1268, 2429, 2686, 3604
Hpy166II GTNNAC 9 cut(s) 630, 1212, 1505, 1790, 1935, 2320, 2481, 3159, 3209
Hpy8I GTNNAC 9 cut(s) 630, 1212, 1505, 1790, 1935, 2320, 2481, 3159, 3209
Hpy99I CGWCG 1 cut(s) 1745
HpyAV CCTTC 7 cut(s) 715, 1757, 1790, 2398, 2665, 2696, 3679
HpyCH4IV ACGT 2 cut(s) 477, 3093
HpySE526I ACGT 2 cut(s) 477, 3093
HspAI GCGC 4 cut(s) 1581, 2501, 3537, 3617
KroI GCCGGC 1 cut(s) 1926
KroNI GCCGGC 1 cut(s) 1928
Ksp22I TGATCA 3 cut(s) 46, 117, 870
LguI GCTCTTC 2 cut(s) 1408, 1998
MaeI CTAG 7 cut(s) 788, 944, 1530, 1586, 1832, 2607, 2901
MaeII ACGT 2 cut(s) 477, 3093
MaeIII GTNAC 5 cut(s) 153, 316, 1981, 2417, 3343
MbiI CCGCTC 1 cut(s) 1167
MfeI CAATTG 4 cut(s) 582, 1032, 1524, 2628
MflI RGATCY 2 cut(s) 1134, 1756
MhlI GDGCHC 2 cut(s) 1420, 2322
MlsI TGGCCA 1 cut(s) 2036
MluNI TGGCCA 1 cut(s) 2036
MlyI GAGTC 9 cut(s) 609, 1284, 1604, 2174, 2912, 2930, 2978, 3191, 3560
MmeI TCCRAC 6 cut(s) 290, 664, 781, 2946, 3115, 3534
Mox20I TGGCCA 1 cut(s) 2036
Mph1103I ATGCAT 2 cut(s) 973, 2791
MroNI GCCGGC 1 cut(s) 1926
MroXI GAANNNNTTC 2 cut(s) 2252, 3288
MscI TGGCCA 1 cut(s) 2036
MslI CAYNNNNRTG 2 cut(s) 113, 2144
Msp20I TGGCCA 1 cut(s) 2036
MspA1I CMGCKG 1 cut(s) 2090
MspI CCGG 6 cut(s) 411, 714, 1827, 1927, 2240, 3071
MspR9I CCNGG 3 cut(s) 412, 1120, 1421
MunI CAATTG 4 cut(s) 582, 1032, 1524, 2628
Mva1269I GAATGC 1 cut(s) 2637
MvaI CCWGG 2 cut(s) 1120, 1421
MvnI CGCG 1 cut(s) 636
NaeI GCCGGC 1 cut(s) 1928
NciI CCSGG 1 cut(s) 412
NcoI CCATGG 1 cut(s) 461
NdeI CATATG 1 cut(s) 1378
NgoMIV GCCGGC 1 cut(s) 1926
NheI GCTAGC 1 cut(s) 2606
NlaIV GGNNCC 8 cut(s) 688, 896, 1877, 2077, 2365, 2707, 2913, 3504
NmeAIII GCCGAG 1 cut(s) 492
NmuCI GTSAC 4 cut(s) 153, 316, 1981, 2417
NsbI TGCGCA 1 cut(s) 3618
NsiI ATGCAT 2 cut(s) 973, 2791
NspI RCATGY 2 cut(s) 1160, 3047
NspV TTCGAA 1 cut(s) 2641
PaeR7I CTCGAG 1 cut(s) 2986
PagI TCATGA 1 cut(s) 165
PaqCI CACCTGC 1 cut(s) 2258
PceI AGGCCT 1 cut(s) 282
PciI ACATGT 1 cut(s) 3043
PciSI GCTCTTC 2 cut(s) 1408, 1998
PcsI WCGNNNNNNNCGW 1 cut(s) 640
PctI GAATGC 1 cut(s) 2637
PdiI GCCGGC 1 cut(s) 1928
PdmI GAANNNNTTC 2 cut(s) 2252, 3288
PfeI GAWTC 5 cut(s) 1146, 1820, 2897, 2959, 3183
PflMI CCANNNNNTGG 1 cut(s) 1253
PinAI ACCGGT 1 cut(s) 1826
PleI GAGTC 9 cut(s) 608, 1283, 1603, 2174, 2912, 2930, 2978, 3191, 3559
PpsI GAGTC 9 cut(s) 608, 1283, 1603, 2174, 2912, 2930, 2978, 3191, 3559
PpuMI RGGWCCY 2 cut(s) 2705, 3502
PscI ACATGT 1 cut(s) 3043
PshAI GACNNNNGTC 2 cut(s) 173, 3201
PshBI ATTAAT 2 cut(s) 1631, 2792
Psp5II RGGWCCY 2 cut(s) 2705, 3502
Psp6I CCWGG 2 cut(s) 1118, 1419
PspFI CCCAGC 1 cut(s) 214
PspGI CCWGG 2 cut(s) 1118, 1419
PspN4I GGNNCC 8 cut(s) 688, 896, 1877, 2077, 2365, 2707, 2913, 3504
PspPI GGNCC 4 cut(s) 895, 2705, 3312, 3502
PspPPI RGGWCCY 2 cut(s) 2705, 3502
PspXI VCTCGAGB 1 cut(s) 2986
PsrI GAACNNNNNNTAC 2 cut(s) 3549, 3581
PstI CTGCAG 1 cut(s) 2089
PstNI CAGNNNCTG 3 cut(s) 659, 751, 1253
PsuI RGATCY 2 cut(s) 1134, 1756
PvuII CAGCTG 1 cut(s) 2090
RseI CAYNNNNRTG 2 cut(s) 113, 2144
SalI GTCGAC 1 cut(s) 3157
SapI GCTCTTC 2 cut(s) 1408, 1998
Sau96I GGNCC 4 cut(s) 895, 2705, 3312, 3502
ScaI AGTACT 1 cut(s) 2547
SchI GAGTC 9 cut(s) 609, 1284, 1604, 2174, 2912, 2930, 2978, 3191, 3560
ScrFI CCNGG 3 cut(s) 412, 1120, 1421
SduI GDGCHC 2 cut(s) 1420, 2322
SfcI CTRYAG 4 cut(s) 666, 1650, 1790, 2085
Sfr274I CTCGAG 1 cut(s) 2986
SfuI TTCGAA 1 cut(s) 2641
SinI GGWCC 3 cut(s) 2705, 3312, 3502
SlaI CTCGAG 1 cut(s) 2986
SmiMI CAYNNNNRTG 2 cut(s) 113, 2144
SmlI CTYRAG 4 cut(s) 240, 1007, 2986, 3411
SmoI CTYRAG 4 cut(s) 240, 1007, 2986, 3411
SseBI AGGCCT 1 cut(s) 282
SsiI CCGC 6 cut(s) 516, 636, 1167, 2159, 2349, 3622
SspI AATATT 2 cut(s) 2952, 3319
SspMI CTAG 7 cut(s) 788, 944, 1530, 1586, 1832, 2607, 2901
StuI AGGCCT 1 cut(s) 282
StyD4I CCNGG 3 cut(s) 410, 1118, 1419
StyI CCWWGG 5 cut(s) 461, 555, 1461, 2221, 2586
TaiI ACGT 2 cut(s) 480, 3096
TatI WGTACW 5 cut(s) 261, 354, 2545, 3208, 3565
TauI GCSGC 1 cut(s) 1170
TfiI GAWTC 5 cut(s) 1146, 1820, 2897, 2959, 3183
TscAI CASTG 6 cut(s) 403, 1707, 3154, 3168, 3546, 3721
TseFI GTSAC 4 cut(s) 153, 316, 1981, 2417
Tsp45I GTSAC 4 cut(s) 153, 316, 1981, 2417
TspRI CASTG 6 cut(s) 403, 1707, 3154, 3168, 3546, 3721
Van91I CCANNNNNTGG 1 cut(s) 1253
VneI GTGCAC 1 cut(s) 2318
VpaK11BI GGWCC 3 cut(s) 2705, 3312, 3502
VspI ATTAAT 2 cut(s) 1631, 2792
XapI RAATTY 9 cut(s) 342, 1554, 2248, 2289, 2353, 2558, 2837, 3103, 3510
XbaI TCTAGA 2 cut(s) 1585, 1831
XceI RCATGY 2 cut(s) 1160, 3047
XcmI CCANNNNNNNNNTGG 1 cut(s) 2029
XhoI CTCGAG 1 cut(s) 2986
XmiI GTMKAC 2 cut(s) 1789, 3158
XmnI GAANNNNTTC 2 cut(s) 2252, 3288
XspI CTAG 7 cut(s) 788, 944, 1530, 1586, 1832, 2607, 2901
ZrmI AGTACT 1 cut(s) 2547
Zsp2I ATGCAT 2 cut(s) 973, 2791
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.